Rh4DG325300

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002719
Physical Location & Seq
Reverse (-)
54985548 .. 54986875
1328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG325300.1

Sequence Viewer

Length: 966 bp
ATGGATCAGCCCAAGCAATCCGCAGTTATCATGCTTTCCTACAGGAGAAAATCCATCGACAACCCCGAAAGGACTTCATGTGCTTCTGATCAAAAGTACTTATTCCGTCCTAAAGCAGGACTACGCATTCCTTGTTCACCGGTAGAGAAGCCAACTCCAGGATCCTGGTCTGAAATCTCGCCTTCAGTCTTTAAGCTCCGTGGGGAGAACTATTTCAAAGACAAACAGAAGTGCCCTGCTCCAAACTACAGCTCATATGTACCAATTGGTATTGATTTGTTTATCTGCCCAAGGAAGAGAGATCACATTGCTCAGCATCTTGAGCTTCCTTCTGTAGAACCACATGACAAAGTTCCTTCCCTCCTTATTGTTAATATACAGTTTCCTACATATCCTATTACAATGTTCCCTAGTGAATGTGATGGTGAAGGCATGAGTCTTGTATTATACTTTAAAGTCAGTGAAAATTTTGACAAAGACATCTCTCCTCAGTTTCAAGAATTCATCAAGAGATTTGTTGAGAATGATACCGAAATGGTTAAGGGGTTCACAAAGGAGTCATCGGTTCCTTTCAGGGAAAGGCTAAAAATTATGGCTGGATTGGTGAATCCAGACGAGGTCCAACTGAGTTCTGCTGAGAAGAAGCTTTTAAGTGCTTATAATGATAAACCAGTGCTTTCGCGTCCTCAACACAATTTCTTCAGGGGACCTAATTATTTTGAGATTGACCTAGACATACACACGTTCAGCTACATCTCCAGGAAAGGGCTTGAATCATCCCGAGACAGATTGAAGAGTGGGGTATTTAATTTGGGATTAACAATCCAGGCACAAAAACAAGAAGAATTGCCGGAGCAAGTCTTGTGTTGCATGCGCTTGAATAAGATTGATTTTGTAAACCATGGTCAAATACCTACTCTTGTTACCATGGATGACTCACTTGGACAAGCACCTGTGGCGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

321

Amino Acids

36.6

Weight (kDa)

7.57

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 56 - 297 5.6e-61 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 660
AccII CGCG 1 cut(s) 682
AciI CCGC 1 cut(s) 21
AclWI GGATC 3 cut(s) 12, 156, 169
AcsI RAATTY 2 cut(s) 466, 500
AcuI CTGAAG 2 cut(s) 168, 685
AcyI GRCGYC 1 cut(s) 959
AfaI GTAC 2 cut(s) 98, 261
AfiI CCNNNNNNNGG 3 cut(s) 116, 158, 765
AflIII ACRYGT 1 cut(s) 741
AgeI ACCGGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 217, 497, 773, 793, 880
AjnI CCWGG 4 cut(s) 157, 164, 758, 825
AluBI AGCT 5 cut(s) 196, 252, 325, 646, 750
AluI AGCT 5 cut(s) 196, 252, 325, 646, 750
Alw26I GTCTC 1 cut(s) 777
AlwI GGATC 3 cut(s) 12, 156, 169
Ama87I CYCGRG 1 cut(s) 780
ApoI RAATTY 2 cut(s) 466, 500
AsiGI ACCGGT 1 cut(s) 139
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 876
AspS9I GGNCC 2 cut(s) 619, 707
AsuHPI GGTGA 3 cut(s) 129, 437, 616
AvaI CYCGRG 1 cut(s) 780
AvaII GGWCC 2 cut(s) 619, 707
BaeGI GKGCMC 1 cut(s) 236
BamHI GGATCC 1 cut(s) 161
BccI CCATC 2 cut(s) 62, 416
BciT130I CCWGG 4 cut(s) 159, 166, 760, 827
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 777
BfaI CTAG 2 cut(s) 411, 731
BfmI CTRYAG 3 cut(s) 40, 247, 333
BlpI GCTNAGC 1 cut(s) 312
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 4 cut(s) 159, 166, 760, 827
Bme18I GGWCC 2 cut(s) 619, 707
BmeT110I CYCGRG 1 cut(s) 780
BmgT120I GGNCC 2 cut(s) 619, 707
BmiI GGNNCC 3 cut(s) 163, 567, 708
BmrFI CCNGG 4 cut(s) 159, 166, 760, 827
BmsI GCATC 1 cut(s) 325
BpmI CTGGAG 2 cut(s) 141, 742
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 959
BsaJI CCNNGG 4 cut(s) 199, 290, 901, 927
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 3 cut(s) 116, 158, 765
Bse118I RCCGGY 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 671
Bse3DI GCAATG 1 cut(s) 306
BseBI CCWGG 4 cut(s) 159, 166, 760, 827
BseDI CCNNGG 4 cut(s) 199, 290, 901, 927
BseGI GGATG 2 cut(s) 776, 937
BseLI CCNNNNNNNGG 3 cut(s) 116, 158, 765
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 4 cut(s) 326, 503, 617, 627
BseNI ACTGG 1 cut(s) 671
BseRI GAGGAG 1 cut(s) 477
BseSI GKGCMC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 682
BshTI ACCGGT 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 780
BsiSI CCGG 2 cut(s) 140, 851
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 3 cut(s) 116, 158, 765
BsmAI GTCTC 1 cut(s) 777
BsmFI GGGAC 1 cut(s) 720
BsmI GAATGC 1 cut(s) 126
BsoBI CYCGRG 1 cut(s) 780
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 4 cut(s) 4, 88, 161, 301
Bsp1720I GCTNAGC 1 cut(s) 312
Bsp19I CCATGG 2 cut(s) 901, 927
BspACI CCGC 1 cut(s) 21
BspCNI CTCAG 4 cut(s) 325, 502, 618, 628
BspFNI CGCG 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 163, 567, 708
BspPI GGATC 3 cut(s) 12, 156, 169
BsrDI GCAATG 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 139
BsrI ACTGG 1 cut(s) 671
BssAI RCCGGY 1 cut(s) 139
BssECI CCNNGG 4 cut(s) 199, 290, 901, 927
BssMI GATC 4 cut(s) 4, 88, 161, 301
BssNI GRCGYC 1 cut(s) 959
BssT1I CCWWGG 3 cut(s) 290, 901, 927
Bst2UI CCWGG 4 cut(s) 159, 166, 760, 827
Bst4CI ACNGT 1 cut(s) 381
Bst6I CTCTTC 2 cut(s) 290, 788
BstACI GRCGYC 1 cut(s) 959
BstC8I GCNNGC 1 cut(s) 872
BstDEI CTNAG 4 cut(s) 312, 489, 626, 636
BstDSI CCRYGG 3 cut(s) 199, 901, 927
BstENI CCTNNNNNAGG 1 cut(s) 114
BstF5I GGATG 2 cut(s) 776, 937
BstFNI CGCG 1 cut(s) 682
BstHHI GCGC 1 cut(s) 876
BstKTI GATC 4 cut(s) 7, 91, 164, 304
BstMAI GTCTC 1 cut(s) 777
BstMBI GATC 4 cut(s) 4, 88, 161, 301
BstMWI GCNNNNNNNGC 2 cut(s) 322, 956
BstNI CCWGG 4 cut(s) 159, 166, 760, 827
BstNSI RCATGY 1 cut(s) 874
BstSCI CCNGG 4 cut(s) 157, 164, 758, 825
BstSFI CTRYAG 3 cut(s) 40, 247, 333
BstSLI GKGCMC 1 cut(s) 236
BstUI CGCG 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 165
BstYI RGATCY 1 cut(s) 161
BtgI CCRYGG 3 cut(s) 199, 901, 927
BtsCI GGATG 2 cut(s) 776, 937
BtsIMutI CAGTG 2 cut(s) 466, 678
Cac8I GCNNGC 1 cut(s) 872
CfoI GCGC 1 cut(s) 876
Cfr10I RCCGGY 1 cut(s) 139
Cfr13I GGNCC 2 cut(s) 619, 707
CseI GACGC 2 cut(s) 671, 948
Csp6I GTAC 2 cut(s) 97, 260
CspAI ACCGGT 1 cut(s) 139
CviAII CATG 7 cut(s) 31, 78, 344, 433, 871, 902, 928
CviQI GTAC 2 cut(s) 97, 260
DdeI CTNAG 4 cut(s) 312, 489, 626, 636
DpnI GATC 4 cut(s) 6, 90, 163, 303
DpnII GATC 4 cut(s) 4, 88, 161, 301
DraI TTTAAA 1 cut(s) 454
Eam1104I CTCTTC 2 cut(s) 290, 788
EarI CTCTTC 2 cut(s) 290, 788
Eco130I CCWWGG 3 cut(s) 290, 901, 927
Eco47I GGWCC 2 cut(s) 619, 707
Eco57I CTGAAG 2 cut(s) 168, 685
Eco88I CYCGRG 1 cut(s) 780
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoO109I RGGNCCY 1 cut(s) 707
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 4 cut(s) 157, 164, 758, 825
EcoT14I CCWWGG 3 cut(s) 290, 901, 927
ErhI CCWWGG 3 cut(s) 290, 901, 927
FaeI CATG 7 cut(s) 34, 81, 347, 436, 874, 905, 931
FaqI GGGAC 1 cut(s) 720
FatI CATG 7 cut(s) 30, 77, 343, 432, 870, 901, 927
FauNDI CATATG 1 cut(s) 256
FbaI TGATCA 1 cut(s) 88
FokI GGATG 2 cut(s) 763, 944
FspBI CTAG 2 cut(s) 411, 731
GlaI GCGC 1 cut(s) 875
GsuI CTGGAG 2 cut(s) 141, 742
HapII CCGG 2 cut(s) 140, 851
HgaI GACGC 2 cut(s) 671, 948
HhaI GCGC 1 cut(s) 876
Hin1I GRCGYC 1 cut(s) 959
Hin1II CATG 7 cut(s) 34, 81, 347, 436, 874, 905, 931
Hin6I GCGC 1 cut(s) 874
HinP1I GCGC 1 cut(s) 874
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 436, 557, 607, 773, 935
HpaII CCGG 2 cut(s) 140, 851
HphI GGTGA 3 cut(s) 129, 437, 616
Hpy166II GTNNAC 3 cut(s) 137, 549, 898
Hpy188I TCNGA 2 cut(s) 88, 172
Hpy188III TCNNGA 6 cut(s) 320, 497, 508, 611, 780, 963
Hpy8I GTNNAC 3 cut(s) 137, 549, 898
HpyAV CCTTC 4 cut(s) 192, 339, 366, 422
HpyCH4III ACNGT 1 cut(s) 381
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 1 cut(s) 870
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 956
HpyF3I CTNAG 4 cut(s) 312, 489, 626, 636
HpySE526I ACGT 1 cut(s) 743
Hsp92I GRCGYC 1 cut(s) 959
Hsp92II CATG 7 cut(s) 34, 81, 347, 436, 874, 905, 931
HspAI GCGC 1 cut(s) 874
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 4 cut(s) 4, 88, 161, 301
LmnI GCTCC 3 cut(s) 201, 244, 853
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 411, 731
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 1 cut(s) 922
MalI GATC 4 cut(s) 6, 90, 163, 303
MboI GATC 4 cut(s) 4, 88, 161, 301
MboII GAAGA 5 cut(s) 307, 652, 691, 805, 854
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 8 cut(s) 264, 466, 500, 588, 694, 712, 808, 845
MlyI GAGTC 3 cut(s) 445, 566, 929
MmeI TCCRAC 1 cut(s) 646
MnlI CCTC 4 cut(s) 371, 498, 610, 696
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 7 cut(s) 192, 372, 453, 540, 650, 807, 818
MspI CCGG 2 cut(s) 140, 851
MspR9I CCNGG 4 cut(s) 159, 166, 760, 827
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 126
MvaI CCWGG 4 cut(s) 159, 166, 760, 827
MvnI CGCG 1 cut(s) 682
MwoI GCNNNNNNNGC 2 cut(s) 322, 956
NcoI CCATGG 2 cut(s) 901, 927
NdeI CATATG 1 cut(s) 256
NdeII GATC 4 cut(s) 4, 88, 161, 301
NlaIII CATG 7 cut(s) 34, 81, 347, 436, 874, 905, 931
NlaIV GGNNCC 3 cut(s) 163, 567, 708
NspI RCATGY 1 cut(s) 874
PaeI GCATGC 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PctI GAATGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 607, 773
PflFI GACNNNGTC 1 cut(s) 617
PfoI TCCNGGA 2 cut(s) 157, 758
PinAI ACCGGT 1 cut(s) 139
PleI GAGTC 3 cut(s) 444, 565, 929
PpsI GAGTC 3 cut(s) 444, 565, 929
PpuMI RGGWCCY 1 cut(s) 707
PsiI TTATAA 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 707
Psp6I CCWGG 4 cut(s) 157, 164, 758, 825
PspGI CCWGG 4 cut(s) 157, 164, 758, 825
PspN4I GGNNCC 3 cut(s) 163, 567, 708
PspPI GGNCC 2 cut(s) 619, 707
PspPPI RGGWCCY 1 cut(s) 707
PsuI RGATCY 1 cut(s) 161
PsyI GACNNNGTC 1 cut(s) 617
RsaI GTAC 2 cut(s) 98, 261
RsaNI GTAC 2 cut(s) 97, 260
SaqAI TTAA 7 cut(s) 192, 372, 453, 540, 650, 807, 818
Sau3AI GATC 4 cut(s) 4, 88, 161, 301
Sau96I GGNCC 2 cut(s) 619, 707
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 3 cut(s) 445, 566, 929
ScrFI CCNGG 4 cut(s) 159, 166, 760, 827
SduI GDGCHC 1 cut(s) 236
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 3 cut(s) 40, 247, 333
SinI GGWCC 2 cut(s) 619, 707
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 1 cut(s) 874
Sse9I AATT 8 cut(s) 264, 466, 500, 588, 694, 712, 808, 845
SsiI CCGC 1 cut(s) 21
SspMI CTAG 2 cut(s) 411, 731
StyD4I CCNGG 4 cut(s) 157, 164, 758, 825
StyI CCWWGG 3 cut(s) 290, 901, 927
TaaI ACNGT 1 cut(s) 381
TaiI ACGT 1 cut(s) 746
TaqI TCGA 1 cut(s) 57
TasI AATT 8 cut(s) 264, 466, 500, 588, 694, 712, 808, 845
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 2 cut(s) 607, 773
Tru1I TTAA 7 cut(s) 192, 372, 453, 540, 650, 807, 818
Tru9I TTAA 7 cut(s) 192, 372, 453, 540, 650, 807, 818
TscAI CASTG 2 cut(s) 466, 678
TspDTI ATGAA 2 cut(s) 66, 493
TspGWI ACGGA 2 cut(s) 95, 188
TspRI CASTG 2 cut(s) 466, 678
Tth111I GACNNNGTC 1 cut(s) 617
VpaK11BI GGWCC 2 cut(s) 619, 707
XagI CCTNNNNNAGG 1 cut(s) 114
XapI RAATTY 2 cut(s) 466, 500
XceI RCATGY 1 cut(s) 874
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 2 cut(s) 411, 731
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.