FvH4_4g33450

Aspartate-semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003893
Physical Location & Seq
Reverse (-)
31688470 .. 31692054
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33450.t1

Sequence Viewer

Length: 1137 bp
ATGGCCGCCGCTTTCGCCAAAACCTCCATGTTCGGCGCCGCCGTCCGCATTCCCTCTCCCACCACCACCGCCACCAACTCCCGTTCCCCATTCCGCGTCCGGATGGCCCTCCAAGACTCCGGCCCCTCCATCGCCGTCGTTGGCGTCACCGGCGCCGTCGGCCAGGAATTCCTCTCCGTCCTCCACGACCGTGACTTCCCCTACCGCTCCATCAAAATGCTCGCCTCCAAGCGCTCCGCCGGCCAAAGCCTCTCCTTCGAAGGCCGCAGCTACACCGTCGAGGAGCTCACCGCCGACAGCTTCGACGGCGTCGACATTGCCCTGTTCAGCGCTGGTGGGTCCATCAGCAAAGAGTTCGGGCCTTTGGCGGTGGGGAAGGGGACGGTGGTGGTGGATAACAGCTCGGCGTTTCGGATGGAGGAGGGGGTCCCACTGGTGGTGCCGGAAGTGAACCCGGAGGCGATGGAGGGGATTAAGGCCGGAAAAGGGAAGGGGGCGCTTATTGCTAATCCGAATTGCTCCACTATTATTTGCTTGATGGCTGCTACTCCTCTGCATCGTCACGCTAAGGTAAAACGGATGGTGGTTAGTACATATCAGGCTGCTAGTGGAGCTGGTGCTGCTGCTATGAGAGAGCTTGAGCAGCAAACTCATGAGGTTTTGGCAGGGAAGCCAGTAACTTGTGAGATATTTAATCGGCAGTATGCTTTTAATTTGTTTTCCCACAATTCAGCGGTTCTTCCAAATGGATATAATGAAGAGGAAATGAAACTAGTCAAGGAGACACGAAAAATATGGAATGACAAGGATGTCAAGGTAACTGCCACATGCATACGAGTTCCTGTAATGCGGGCACATGCTGAGAGTGTAAATCTTGAATTTGAGAATCCCCTTGATGAGGACACTGCAAGGGATATTCTGAAGAAGGCTCCTGGTGTGGTGGTTATAGATGACCGTGCAGCTAATAACTTCCCTACACCTCTGGAGGTGTCAAACAAAGATGATGTTGCCGTTGGCAGAATCCGCCGTGATGTGTCCCAAGAGGGCAACTATGGGTTGGACATCTTTGTCTGTGGTGATCAAATACGCAAGGGAGCTGCACTCAATGCTGTTCAGATTGCTGAGCTGTTGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230
Pfam Domains
Protein Families
Semialdhyde_dh Semialdhyde_dhC

Protein Analysis

379

Amino Acids

40.63

Weight (kDa)

6.35

Isoelectric Point (pI)

32.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dh PF01118 44 - 156 1.6e-33 Semialdehyde dehydrogenase, NAD binding domain
Semialdhyde_dhC PF02774 184 - 364 1.6e-46 Semialdehyde dehydrogenase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 809, 1067
AccB1I GGYRCC 3 cut(s) 35, 152, 439
AccBSI CCGCTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 96
AccIII TCCGGA 1 cut(s) 99
AcoI YGGCCR 3 cut(s) 3, 160, 241
AcsI RAATTY 2 cut(s) 167, 878
AcuI CTGAAG 1 cut(s) 941
AcyI GRCGYC 4 cut(s) 36, 144, 153, 309
AfaI GTAC 1 cut(s) 592
AfeI AGCGCT 2 cut(s) 233, 331
AfiI CCNNNNNNNGG 3 cut(s) 436, 486, 898
AgsI TTSAA 1 cut(s) 878
AhlI ACTAGT 1 cut(s) 772
AjnI CCWGG 2 cut(s) 162, 931
AleI CACNNNNGTG 1 cut(s) 189
AluBI AGCT 9 cut(s) 270, 286, 300, 402, 614, 637, 962, 1097, 1126
AluI AGCT 9 cut(s) 270, 286, 300, 402, 614, 637, 962, 1097, 1126
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 776
Aor13HI TCCGGA 1 cut(s) 99
Aor51HI AGCGCT 2 cut(s) 233, 331
AoxI GGCC 8 cut(s) 3, 105, 121, 160, 241, 262, 359, 477
ApeKI GCWGC 8 cut(s) 267, 542, 602, 620, 623, 643, 959, 1097
ApoI RAATTY 2 cut(s) 167, 878
AspLEI GCGC 5 cut(s) 38, 155, 234, 332, 499
AspS9I GGNCC 5 cut(s) 106, 122, 339, 359, 427
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 3 cut(s) 139, 280, 1088
AsuII TTCGAA 1 cut(s) 258
AvaII GGWCC 2 cut(s) 339, 427
BaeGI GKGCMC 1 cut(s) 856
BanI GGYRCC 3 cut(s) 35, 152, 439
BanII GRGCYC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 8 cut(s) 279, 529, 589, 607, 610, 655, 971, 1084
BccI CCATC 8 cut(s) 97, 137, 218, 350, 409, 457, 532, 574
BceAI ACGGC 6 cut(s) 26, 119, 140, 322, 995, 1011
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 2 cut(s) 164, 933
BclI TGATCA 1 cut(s) 1078
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 1 cut(s) 776
BcuI ACTAGT 1 cut(s) 772
BfaI CTAG 2 cut(s) 606, 773
BfoI RGCGCY 5 cut(s) 39, 156, 235, 333, 500
BlpI GCTNAGC 1 cut(s) 1122
Bme1390I CCNGG 3 cut(s) 164, 455, 933
Bme18I GGWCC 2 cut(s) 339, 427
BmgT120I GGNCC 5 cut(s) 106, 122, 339, 359, 427
BmiI GGNNCC 8 cut(s) 37, 124, 154, 340, 428, 429, 441, 930
BmrFI CCNGG 3 cut(s) 164, 455, 933
BmsI GCATC 1 cut(s) 565
BplI GAGNNNNNCTC 2 cut(s) 1086, 1118
BpmI CTGGAG 1 cut(s) 1004
Bpu10I CCTNAGC 1 cut(s) 567
Bpu1102I GCTNAGC 1 cut(s) 1122
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 659
BpuMI CCSGG 1 cut(s) 455
BsaHI GRCGYC 4 cut(s) 36, 144, 153, 309
BsaWI WCCGGW 1 cut(s) 99
Bsc4I CCNNNNNNNGG 3 cut(s) 436, 486, 898
Bse118I RCCGGY 2 cut(s) 149, 239
Bse1I ACTGG 2 cut(s) 438, 674
Bse3DI GCAATG 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 99
BseBI CCWGG 2 cut(s) 164, 933
BseGI GGATG 4 cut(s) 108, 420, 585, 814
BseLI CCNNNNNNNGG 3 cut(s) 436, 486, 898
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 852, 1113
BseNI ACTGG 2 cut(s) 438, 674
BseRI GAGGAG 3 cut(s) 296, 434, 540
BseSI GKGCMC 1 cut(s) 856
BseXI GCAGC 8 cut(s) 279, 529, 589, 607, 610, 655, 971, 1084
BsgI GTGCAG 2 cut(s) 978, 1083
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 8 cut(s) 5, 107, 123, 162, 243, 264, 361, 479
BshNI GGYRCC 3 cut(s) 35, 152, 439
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 288
BsiSI CCGG 7 cut(s) 100, 120, 150, 240, 443, 455, 480
BslFI GGGAC 3 cut(s) 394, 413, 1021
BslI CCNNNNNNNGG 3 cut(s) 436, 486, 898
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 3 cut(s) 394, 413, 1021
BsmI GAATGC 1 cut(s) 48
BsnI GGCC 8 cut(s) 5, 107, 123, 162, 243, 264, 361, 479
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 2 cut(s) 288, 856
Bsp13I TCCGGA 1 cut(s) 99
Bsp143I GATC 1 cut(s) 1078
Bsp1720I GCTNAGC 1 cut(s) 1122
BspANI GGCC 8 cut(s) 5, 107, 123, 162, 243, 264, 361, 479
BspCNI CTCAG 2 cut(s) 853, 1114
BspEI TCCGGA 1 cut(s) 99
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 8 cut(s) 37, 124, 154, 340, 428, 429, 441, 930
BspT104I TTCGAA 1 cut(s) 258
BspT107I GGYRCC 3 cut(s) 35, 152, 439
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 315
BsrFI RCCGGY 2 cut(s) 149, 239
BsrI ACTGG 2 cut(s) 438, 674
BssAI RCCGGY 2 cut(s) 149, 239
BssMI GATC 1 cut(s) 1078
BssNI GRCGYC 4 cut(s) 36, 144, 153, 309
Bst2UI CCWGG 2 cut(s) 164, 933
Bst4CI ACNGT 4 cut(s) 191, 277, 385, 956
Bst6I CTCTTC 1 cut(s) 753
BstACI GRCGYC 4 cut(s) 36, 144, 153, 309
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 222, 241, 852
BstDEI CTNAG 3 cut(s) 567, 861, 1122
BstENI CCTNNNNNAGG 1 cut(s) 896
BstF5I GGATG 4 cut(s) 108, 420, 585, 814
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 5 cut(s) 39, 156, 235, 333, 500
BstHHI GCGC 5 cut(s) 38, 155, 234, 332, 499
BstKTI GATC 1 cut(s) 1081
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 1 cut(s) 1078
BstMCI CGRYCG 1 cut(s) 190
BstNI CCWGG 2 cut(s) 164, 933
BstNSI RCATGY 2 cut(s) 831, 860
BstSCI CCNGG 3 cut(s) 162, 453, 931
BstSLI GKGCMC 1 cut(s) 856
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 8 cut(s) 279, 529, 589, 607, 610, 655, 971, 1084
BsuRI GGCC 8 cut(s) 5, 107, 123, 162, 243, 264, 361, 479
BtgZI GCGATG 2 cut(s) 115, 476
BtsCI GGATG 4 cut(s) 108, 420, 585, 814
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 431, 903
Cac8I GCNNGC 3 cut(s) 222, 241, 852
CciI TCATGA 1 cut(s) 652
CfoI GCGC 5 cut(s) 38, 155, 234, 332, 499
Cfr10I RCCGGY 2 cut(s) 149, 239
Cfr13I GGNCC 5 cut(s) 106, 122, 339, 359, 427
CseI GACGC 3 cut(s) 85, 133, 298
Csp6I GTAC 1 cut(s) 591
CviAII CATG 4 cut(s) 28, 653, 828, 857
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 3 cut(s) 567, 861, 1122
DinI GGCGCC 2 cut(s) 37, 154
DpnI GATC 1 cut(s) 1080
DpnII GATC 1 cut(s) 1078
DrdI GACNNNNNNGTC 2 cut(s) 809, 1067
DseDI GACNNNNNNGTC 2 cut(s) 809, 1067
EaeI YGGCCR 3 cut(s) 3, 160, 241
Eam1104I CTCTTC 1 cut(s) 753
EarI CTCTTC 1 cut(s) 753
EciI GGCGGA 2 cut(s) 226, 1013
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 2 cut(s) 339, 427
Eco47III AGCGCT 2 cut(s) 233, 331
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 896
EcoO109I RGGNCCY 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 162, 931
EcoT22I ATGCAT 1 cut(s) 833
EcoT38I GRGCYC 1 cut(s) 288
EgeI GGCGCC 2 cut(s) 37, 154
EheI GGCGCC 2 cut(s) 37, 154
FaeI CATG 4 cut(s) 31, 656, 831, 860
FaqI GGGAC 3 cut(s) 394, 413, 1021
FatI CATG 4 cut(s) 27, 652, 827, 856
FauI CCCGC 1 cut(s) 843
FbaI TGATCA 1 cut(s) 1078
FblI GTMKAC 1 cut(s) 312
FokI GGATG 4 cut(s) 115, 427, 592, 821
FriOI GRGCYC 1 cut(s) 288
FspBI CTAG 2 cut(s) 606, 773
GlaI GCGC 5 cut(s) 37, 154, 233, 331, 498
GsuI CTGGAG 1 cut(s) 1004
HaeII RGCGCY 5 cut(s) 39, 156, 235, 333, 500
HaeIII GGCC 8 cut(s) 5, 107, 123, 162, 243, 264, 361, 479
HapII CCGG 7 cut(s) 100, 120, 150, 240, 443, 455, 480
HgaI GACGC 3 cut(s) 85, 133, 298
HhaI GCGC 5 cut(s) 38, 155, 234, 332, 499
Hin1I GRCGYC 4 cut(s) 36, 144, 153, 309
Hin1II CATG 4 cut(s) 31, 656, 831, 860
Hin6I GCGC 5 cut(s) 36, 153, 232, 330, 497
HinP1I GCGC 5 cut(s) 36, 153, 232, 330, 497
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HinfI GANTC 3 cut(s) 116, 886, 1020
HpaII CCGG 7 cut(s) 100, 120, 150, 240, 443, 455, 480
HphI GGTGA 3 cut(s) 139, 280, 1088
Hpy166II GTNNAC 2 cut(s) 313, 451
Hpy188I TCNGA 4 cut(s) 414, 513, 921, 1116
Hpy188III TCNNGA 4 cut(s) 100, 653, 875, 983
Hpy8I GTNNAC 2 cut(s) 313, 451
Hpy99I CGWCG 5 cut(s) 140, 161, 281, 308, 314
HpyAV CCTTC 5 cut(s) 254, 265, 370, 484, 919
HpyCH4III ACNGT 4 cut(s) 191, 277, 385, 956
HpyCH4V TGCA 5 cut(s) 556, 831, 908, 959, 1100
HpyF3I CTNAG 3 cut(s) 567, 861, 1122
Hsp92I GRCGYC 4 cut(s) 36, 144, 153, 309
Hsp92II CATG 4 cut(s) 31, 656, 831, 860
HspAI GCGC 5 cut(s) 36, 153, 232, 330, 497
KasI GGCGCC 2 cut(s) 35, 152
KflI GGGWCCC 1 cut(s) 427
Kpn2I TCCGGA 1 cut(s) 99
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 1078
Kzo9I GATC 1 cut(s) 1078
LmnI GCTCC 7 cut(s) 212, 239, 283, 524, 611, 934, 1094
Lsp1109I GCAGC 8 cut(s) 279, 529, 589, 607, 610, 655, 971, 1084
LweI GCATC 1 cut(s) 565
MaeI CTAG 2 cut(s) 606, 773
MaeIII GTNAC 5 cut(s) 145, 191, 560, 676, 817
MalI GATC 1 cut(s) 1080
MbiI CCGCTC 1 cut(s) 207
MboI GATC 1 cut(s) 1078
MboII GAAGA 3 cut(s) 731, 770, 934
MhlI GDGCHC 2 cut(s) 288, 856
MluCI AATT 5 cut(s) 167, 514, 712, 727, 878
Mly113I GGCGCC 2 cut(s) 36, 153
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 1038
Mph1103I ATGCAT 1 cut(s) 833
MroI TCCGGA 1 cut(s) 99
MroNI GCCGGC 1 cut(s) 239
MseI TTAA 3 cut(s) 474, 693, 711
MslI CAYNNNNRTG 2 cut(s) 189, 215
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 7 cut(s) 100, 120, 150, 240, 443, 455, 480
MspR9I CCNGG 3 cut(s) 164, 455, 933
Mva1269I GAATGC 1 cut(s) 48
MvaI CCWGG 2 cut(s) 164, 933
MvnI CGCG 1 cut(s) 96
NaeI GCCGGC 1 cut(s) 241
NarI GGCGCC 2 cut(s) 36, 153
NciI CCSGG 1 cut(s) 455
NdeII GATC 1 cut(s) 1078
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 4 cut(s) 31, 656, 831, 860
NlaIV GGNNCC 8 cut(s) 37, 124, 154, 340, 428, 429, 441, 930
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 3 cut(s) 145, 191, 560
NsiI ATGCAT 1 cut(s) 833
NspI RCATGY 2 cut(s) 831, 860
NspV TTCGAA 1 cut(s) 258
OliI CACNNNNGTG 1 cut(s) 189
PagI TCATGA 1 cut(s) 652
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 309
PctI GAATGC 1 cut(s) 48
PdiI GCCGGC 1 cut(s) 241
PfeI GAWTC 2 cut(s) 886, 1020
PflFI GACNNNGTC 1 cut(s) 308
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 2 cut(s) 39, 156
PpsI GAGTC 1 cut(s) 110
PpuMI RGGWCCY 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 2 cut(s) 162, 931
PspGI CCWGG 2 cut(s) 162, 931
PspN4I GGNNCC 8 cut(s) 37, 124, 154, 340, 428, 429, 441, 930
PspPI GGNCC 5 cut(s) 106, 122, 339, 359, 427
PspPPI RGGWCCY 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 2 cut(s) 189, 215
SacI GAGCTC 1 cut(s) 288
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 3 cut(s) 474, 693, 711
Sau3AI GATC 1 cut(s) 1078
Sau96I GGNCC 5 cut(s) 106, 122, 339, 359, 427
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 3 cut(s) 164, 455, 933
SduI GDGCHC 2 cut(s) 288, 856
SfaNI GCATC 1 cut(s) 565
SfoI GGCGCC 2 cut(s) 37, 154
SfuI TTCGAA 1 cut(s) 258
SgrAI CRCCGGYG 1 cut(s) 149
SinI GGWCC 2 cut(s) 339, 427
SmiMI CAYNNNNRTG 2 cut(s) 189, 215
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
SpeI ACTAGT 1 cut(s) 772
Sse9I AATT 5 cut(s) 167, 514, 712, 727, 878
SspDI GGCGCC 2 cut(s) 35, 152
SspMI CTAG 2 cut(s) 606, 773
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 162, 453, 931
TaaI ACNGT 4 cut(s) 191, 277, 385, 956
TaqI TCGA 4 cut(s) 258, 279, 303, 312
TasI AATT 5 cut(s) 167, 514, 712, 727, 878
TatI WGTACW 1 cut(s) 590
TauI GCSGC 4 cut(s) 8, 11, 41, 267
TfiI GAWTC 2 cut(s) 886, 1020
Tru1I TTAA 3 cut(s) 474, 693, 711
Tru9I TTAA 3 cut(s) 474, 693, 711
TscAI CASTG 2 cut(s) 438, 910
TseFI GTSAC 3 cut(s) 145, 191, 560
TseI GCWGC 8 cut(s) 267, 542, 602, 620, 623, 643, 959, 1097
Tsp45I GTSAC 3 cut(s) 145, 191, 560
TspDTI ATGAA 2 cut(s) 771, 782
TspGWI ACGGA 2 cut(s) 166, 592
TspRI CASTG 2 cut(s) 438, 910
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 339, 427
XagI CCTNNNNNAGG 1 cut(s) 896
XapI RAATTY 2 cut(s) 167, 878
XceI RCATGY 2 cut(s) 831, 860
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 2 cut(s) 606, 773
Zsp2I ATGCAT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.