pycom13g01660

Aspartate-semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003893
Physical Location & Seq
Reverse (-)
1097068 .. 1099396
2329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g01660.2

Sequence Viewer

Length: 1251 bp
ATGAATGTGCAAGTTAAAATTGAAGTCGTGCCCTTCGAGCAAAACTCAAAACCCCACTCCGCCGCCGCCGCCACTCGTTTCTCCTCCGGCTCCAACCAAATCTCCAAAATGTCCACTTTCACCCACCAACACAATGGCCTCCTCCTCAAGTCGCCCCTCTCGGGCCGGTCTCGGGTCCCCGTGTCCAGGTTCCGACCCGGTCCATTCACAGTCCGAATGTCTCTCCAAGAGACCGGTCCATCACTCGCCGTGGTCGGCGTTACCGGCGCAGTCGGTCAGGAGTTCCTCTCCGTCCTCCAAGACCGCGACTTTCCTTTCCGTTCAATAAAGATGCTCGCGTCCAAGCGCTCCGCTGGTCAGAACTTGAACTTCCTCGGCCAAGACTACGTCGTTGAGGAGCTCACGCACGACAGCTTTGACGGCGTCGATATCGCCCTCTTCAGCGCCGGCGGGTCAATCAGCAAGGAGTTCGGTCCCGTAGCGGTAGAGCGCGGCACCATTGTCGTTGATAACAGCTCTGCTTTCCGGATGCAAAATGGCGTGCCGCTTGTGGTCCCTGAAGTCAATCCGGAGGCTATGGAGGGAATCAAGGTTGGGAAGGGAAAGGGGGCGCTTATTGCTAACCCAAATTGCTCCACCATTATTTGTTTAATGGCTGTCACCCCTCTCCATCGCCATTCTAAGGTAAAACGAATGGTGGTTAGCACCTATCAGGCTGCTAGTGGAGCTGGTGCTGCTGCCATGAGAGAGCTTGAGCTGCAAACTAAGGAGGTCCTGGAAGGGAAGCCACCAACCACTAAGATATTTTCGCGACAGTATGCGTTTAATTTGTTTTCGCACAACTCGGCAGTTCTTTCGAATGGATATAATGAAGAGGAAATGAAACTAGTCAAGGAGACACGAAAAATATGGAGTGACAATGATGTCAAGGTAACTGCCACATGTATACGAGTTCCTGTCATGCGGGCGCATGCTGAGAGTATAAATCTTCATTTTGAGAACCAACTTGACGAGGACACAGCAAGGGAAATTCTGAAGAAAGCGCCTGGGCTGGTGGTTATTGATGACCGGTCAGCGAATAACTTCCCTACACCATTGGAGGTGTCAAACAAAGACGATGTTGCAGTAGGCAGAATCCGTCGTGATGTGTCCCAAGAGGGGAACTATGGGTTGGATATCTTTGTCTGCGGTGATCAAATACGCAAGGGAGCTGCACTTAATGCTGTTCAGATTGCCGAGATGTTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230
Pfam Domains
Protein Families
Semialdhyde_dh Semialdhyde_dhC

Protein Analysis

417

Amino Acids

45.31

Weight (kDa)

8.56

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 923
AccB1I GGYRCC 1 cut(s) 494
AccI GTMKAC 1 cut(s) 946
AccII CGCG 4 cut(s) 306, 338, 492, 811
AccIII TCCGGA 2 cut(s) 525, 568
AcoI YGGCCR 1 cut(s) 376
AcsI RAATTY 1 cut(s) 1029
AcuI CTGAAG 3 cut(s) 424, 579, 1055
AcyI GRCGYC 1 cut(s) 423
AfeI AGCGCT 1 cut(s) 347
AfiI CCNNNNNNNGG 5 cut(s) 161, 172, 186, 682, 1158
AflIII ACRYGT 1 cut(s) 941
AgeI ACCGGT 2 cut(s) 233, 1068
AgsI TTSAA 3 cut(s) 23, 324, 367
AhlI ACTAGT 1 cut(s) 886
AjnI CCWGG 3 cut(s) 185, 774, 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AluBI AGCT 7 cut(s) 400, 414, 516, 728, 751, 757, 1211
AluI AGCT 7 cut(s) 400, 414, 516, 728, 751, 757, 1211
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 4 cut(s) 174, 224, 225, 890
Ama87I CYCGRG 2 cut(s) 160, 171
Aor13HI TCCGGA 2 cut(s) 525, 568
Aor51HI AGCGCT 1 cut(s) 347
AoxI GGCC 3 cut(s) 136, 163, 376
ApeKI GCWGC 5 cut(s) 716, 734, 737, 757, 1211
ApoI RAATTY 1 cut(s) 1029
AsiGI ACCGGT 2 cut(s) 233, 1068
Asp700I GAANNNNTTC 1 cut(s) 1082
AspLEI GCGC 7 cut(s) 269, 348, 446, 492, 613, 970, 1045
AspS9I GGNCC 7 cut(s) 163, 175, 200, 236, 473, 553, 772
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 3 cut(s) 112, 652, 1202
AsuII TTCGAA 1 cut(s) 857
AvaI CYCGRG 2 cut(s) 160, 171
AvaII GGWCC 6 cut(s) 175, 200, 236, 473, 553, 772
BaeGI GKGCMC 1 cut(s) 33
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 5 cut(s) 703, 721, 724, 744, 1198
BccI CCATC 2 cut(s) 247, 678
BceAI ACGGC 2 cut(s) 233, 436
BcgI CGANNNNNNTGC 6 cut(s) 451, 484, 485, 518, 837, 871
BciT130I CCWGG 3 cut(s) 187, 776, 1047
BclI TGATCA 1 cut(s) 1192
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 4 cut(s) 174, 224, 225, 890
BcuI ACTAGT 1 cut(s) 886
BfaI CTAG 2 cut(s) 720, 887
BfoI RGCGCY 4 cut(s) 349, 447, 614, 1046
Bme1390I CCNGG 4 cut(s) 187, 198, 776, 1047
Bme18I GGWCC 6 cut(s) 175, 200, 236, 473, 553, 772
BmeT110I CYCGRG 2 cut(s) 160, 171
BmgT120I GGNCC 7 cut(s) 163, 175, 200, 236, 473, 553, 772
BmiI GGNNCC 7 cut(s) 91, 176, 177, 191, 475, 496, 555
BmrFI CCNGG 4 cut(s) 187, 198, 776, 1047
BmsI GCATC 2 cut(s) 321, 519
BplI GAGNNNNNCTC 4 cut(s) 29, 61, 272, 304
Bpu14I TTCGAA 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 131, 773
BpuMI CCSGG 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 423
BsaI GGTCTC 2 cut(s) 174, 224
BsaJI CCNNGG 3 cut(s) 249, 373, 1046
BsaWI WCCGGW 4 cut(s) 233, 525, 568, 1068
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 172, 186, 682, 1158
Bse118I RCCGGY 5 cut(s) 165, 233, 263, 446, 1068
BseAI TCCGGA 2 cut(s) 525, 568
BseBI CCWGG 3 cut(s) 187, 776, 1047
BseDI CCNNGG 3 cut(s) 249, 373, 1046
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 5 cut(s) 161, 172, 186, 682, 1158
BseMII CTCAG 1 cut(s) 966
BseRI GAGGAG 4 cut(s) 73, 131, 134, 410
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 5 cut(s) 703, 721, 724, 744, 1198
BsgI GTGCAG 1 cut(s) 1197
Bsh1236I CGCG 4 cut(s) 306, 338, 492, 811
BshFI GGCC 3 cut(s) 138, 165, 378
BshNI GGYRCC 1 cut(s) 494
BshTI ACCGGT 2 cut(s) 233, 1068
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 2 cut(s) 160, 171
BsiSI CCGG 9 cut(s) 87, 166, 198, 234, 264, 447, 526, 569, 1069
BslFI GGGAC 4 cut(s) 161, 459, 539, 1135
BslI CCNNNNNNNGG 5 cut(s) 161, 172, 186, 682, 1158
BsmAI GTCTC 4 cut(s) 174, 224, 225, 890
BsmFI GGGAC 4 cut(s) 161, 459, 539, 1135
BsnI GGCC 3 cut(s) 138, 165, 378
Bso31I GGTCTC 2 cut(s) 174, 224
BsoBI CYCGRG 2 cut(s) 160, 171
Bsp119I TTCGAA 1 cut(s) 857
Bsp1286I GDGCHC 2 cut(s) 33, 402
Bsp13I TCCGGA 2 cut(s) 525, 568
Bsp143I GATC 1 cut(s) 1192
Bsp68I TCGCGA 1 cut(s) 811
BspANI GGCC 3 cut(s) 138, 165, 378
BspCNI CTCAG 1 cut(s) 967
BspEI TCCGGA 2 cut(s) 525, 568
BspFNI CGCG 4 cut(s) 306, 338, 492, 811
BspLI GGNNCC 7 cut(s) 91, 176, 177, 191, 475, 496, 555
BspT104I TTCGAA 1 cut(s) 857
BspT107I GGYRCC 1 cut(s) 494
BspTNI GGTCTC 2 cut(s) 174, 224
BsrFI RCCGGY 5 cut(s) 165, 233, 263, 446, 1068
BssAI RCCGGY 5 cut(s) 165, 233, 263, 446, 1068
BssECI CCNNGG 3 cut(s) 249, 373, 1046
BssMI GATC 1 cut(s) 1192
BssNAI GTATAC 1 cut(s) 947
BssNI GRCGYC 1 cut(s) 423
Bst1107I GTATAC 1 cut(s) 947
Bst2UI CCWGG 3 cut(s) 187, 776, 1047
Bst4CI ACNGT 2 cut(s) 211, 816
Bst6I CTCTTC 2 cut(s) 443, 867
BstACI GRCGYC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstBI TTCGAA 1 cut(s) 857
BstC8I GCNNGC 5 cut(s) 336, 448, 542, 966, 972
BstDEI CTNAG 4 cut(s) 681, 765, 798, 975
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 534
BstFNI CGCG 4 cut(s) 306, 338, 492, 811
BstH2I RGCGCY 4 cut(s) 349, 447, 614, 1046
BstHHI GCGC 7 cut(s) 269, 348, 446, 492, 613, 970, 1045
BstKTI GATC 1 cut(s) 1195
BstMAI GTCTC 4 cut(s) 174, 224, 225, 890
BstMBI GATC 1 cut(s) 1192
BstMWI GCNNNNNNNGC 9 cut(s) 37, 68, 264, 420, 617, 725, 734, 757, 1220
BstNI CCWGG 3 cut(s) 187, 776, 1047
BstNSI RCATGY 2 cut(s) 945, 974
BstSCI CCNGG 4 cut(s) 185, 196, 774, 1045
BstSLI GKGCMC 1 cut(s) 33
BstUI CGCG 4 cut(s) 306, 338, 492, 811
BstV1I GCAGC 5 cut(s) 703, 721, 724, 744, 1198
BstXI CCANNNNNNTGG 1 cut(s) 134
BstZ17I GTATAC 1 cut(s) 947
BsuRI GGCC 3 cut(s) 138, 165, 378
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 1 cut(s) 656
BtsCI GGATG 1 cut(s) 534
BtuMI TCGCGA 1 cut(s) 811
Cac8I GCNNGC 5 cut(s) 336, 448, 542, 966, 972
CfoI GCGC 7 cut(s) 269, 348, 446, 492, 613, 970, 1045
Cfr10I RCCGGY 5 cut(s) 165, 233, 263, 446, 1068
Cfr13I GGNCC 7 cut(s) 163, 175, 200, 236, 473, 553, 772
CseI GACGC 2 cut(s) 327, 412
CspAI ACCGGT 2 cut(s) 233, 1068
CviAII CATG 4 cut(s) 742, 942, 961, 971
DdeI CTNAG 4 cut(s) 681, 765, 798, 975
DpnI GATC 1 cut(s) 1194
DpnII GATC 1 cut(s) 1192
DrdI GACNNNNNNGTC 1 cut(s) 923
DseDI GACNNNNNNGTC 1 cut(s) 923
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 2 cut(s) 443, 867
EarI CTCTTC 2 cut(s) 443, 867
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 2 cut(s) 174, 224
Eco32I GATATC 2 cut(s) 430, 1177
Eco47I GGWCC 6 cut(s) 175, 200, 236, 473, 553, 772
Eco47III AGCGCT 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 3 cut(s) 424, 579, 1055
Eco88I CYCGRG 2 cut(s) 160, 171
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 2 cut(s) 175, 772
EcoRII CCWGG 3 cut(s) 185, 774, 1045
EcoRV GATATC 2 cut(s) 430, 1177
EcoT38I GRGCYC 1 cut(s) 402
FaeI CATG 4 cut(s) 745, 945, 964, 974
FaqI GGGAC 4 cut(s) 161, 459, 539, 1135
FatI CATG 4 cut(s) 741, 941, 960, 970
FauI CCCGC 2 cut(s) 443, 957
FbaI TGATCA 1 cut(s) 1192
FblI GTMKAC 1 cut(s) 946
FokI GGATG 1 cut(s) 541
FriOI GRGCYC 1 cut(s) 402
FspBI CTAG 2 cut(s) 720, 887
GlaI GCGC 7 cut(s) 268, 347, 445, 491, 612, 969, 1044
HaeII RGCGCY 4 cut(s) 349, 447, 614, 1046
HaeIII GGCC 3 cut(s) 138, 165, 378
HapII CCGG 9 cut(s) 87, 166, 198, 234, 264, 447, 526, 569, 1069
HgaI GACGC 2 cut(s) 327, 412
HhaI GCGC 7 cut(s) 269, 348, 446, 492, 613, 970, 1045
Hin1I GRCGYC 1 cut(s) 423
Hin1II CATG 4 cut(s) 745, 945, 964, 974
Hin6I GCGC 7 cut(s) 267, 346, 444, 490, 611, 968, 1043
HinP1I GCGC 7 cut(s) 267, 346, 444, 490, 611, 968, 1043
HinfI GANTC 2 cut(s) 585, 1134
HpaII CCGG 9 cut(s) 87, 166, 198, 234, 264, 447, 526, 569, 1069
HphI GGTGA 3 cut(s) 112, 652, 1202
Hpy166II GTNNAC 2 cut(s) 114, 947
Hpy188I TCNGA 5 cut(s) 194, 215, 360, 1035, 1230
Hpy188III TCNNGA 5 cut(s) 278, 526, 569, 810, 1142
Hpy8I GTNNAC 2 cut(s) 114, 947
Hpy99I CGWCG 3 cut(s) 392, 428, 1143
HpyAV CCTTC 3 cut(s) 43, 592, 773
HpyCH4III ACNGT 2 cut(s) 211, 816
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 5 cut(s) 10, 532, 760, 1124, 1214
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 68, 264, 420, 617, 725, 734, 757, 1220
HpyF3I CTNAG 4 cut(s) 681, 765, 798, 975
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 423
Hsp92II CATG 4 cut(s) 745, 945, 964, 974
HspAI GCGC 7 cut(s) 267, 346, 444, 490, 611, 968, 1043
KflI GGGWCCC 1 cut(s) 175
Kpn2I TCCGGA 2 cut(s) 525, 568
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 1192
Kzo9I GATC 1 cut(s) 1192
LmnI GCTCC 6 cut(s) 95, 353, 397, 638, 725, 1208
Lsp1109I GCAGC 5 cut(s) 703, 721, 724, 744, 1198
LweI GCATC 2 cut(s) 321, 519
MaeI CTAG 2 cut(s) 720, 887
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 4 cut(s) 259, 658, 914, 931
MalI GATC 1 cut(s) 1194
MboI GATC 1 cut(s) 1192
MboII GAAGA 4 cut(s) 430, 884, 980, 1048
MhlI GDGCHC 2 cut(s) 33, 402
MluCI AATT 4 cut(s) 18, 628, 826, 1029
MmeI TCCRAC 3 cut(s) 117, 217, 1152
MreI CGCCGGCG 1 cut(s) 446
MroI TCCGGA 2 cut(s) 525, 568
MroNI GCCGGC 1 cut(s) 446
MroXI GAANNNNTTC 1 cut(s) 1082
MseI TTAA 4 cut(s) 15, 650, 825, 1218
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 9 cut(s) 87, 166, 198, 234, 264, 447, 526, 569, 1069
MspR9I CCNGG 4 cut(s) 187, 198, 776, 1047
MvaI CCWGG 3 cut(s) 187, 776, 1047
MvnI CGCG 4 cut(s) 306, 338, 492, 811
MwoI GCNNNNNNNGC 9 cut(s) 37, 68, 264, 420, 617, 725, 734, 757, 1220
NaeI GCCGGC 1 cut(s) 448
NciI CCSGG 1 cut(s) 198
NdeII GATC 1 cut(s) 1192
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 4 cut(s) 745, 945, 964, 974
NlaIV GGNNCC 7 cut(s) 91, 176, 177, 191, 475, 496, 555
NmeAIII GCCGAG 2 cut(s) 354, 824
NmuCI GTSAC 2 cut(s) 658, 914
NruI TCGCGA 1 cut(s) 811
NspI RCATGY 2 cut(s) 945, 974
NspV TTCGAA 1 cut(s) 857
PaeI GCATGC 1 cut(s) 974
PciI ACATGT 1 cut(s) 941
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdiI GCCGGC 1 cut(s) 448
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 2 cut(s) 585, 1134
PflFI GACNNNGTC 3 cut(s) 198, 386, 422
PfoI TCCNGGA 1 cut(s) 774
PinAI ACCGGT 2 cut(s) 233, 1068
PpuMI RGGWCCY 2 cut(s) 175, 772
PscI ACATGT 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 402
Psp5II RGGWCCY 2 cut(s) 175, 772
Psp6I CCWGG 3 cut(s) 185, 774, 1045
PspGI CCWGG 3 cut(s) 185, 774, 1045
PspN4I GGNNCC 7 cut(s) 91, 176, 177, 191, 475, 496, 555
PspPI GGNCC 7 cut(s) 163, 175, 200, 236, 473, 553, 772
PspPPI RGGWCCY 2 cut(s) 175, 772
PsyI GACNNNGTC 3 cut(s) 198, 386, 422
RruI TCGCGA 1 cut(s) 811
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 4 cut(s) 15, 650, 825, 1218
Sau3AI GATC 1 cut(s) 1192
Sau96I GGNCC 7 cut(s) 163, 175, 200, 236, 473, 553, 772
ScrFI CCNGG 4 cut(s) 187, 198, 776, 1047
SduI GDGCHC 2 cut(s) 33, 402
SfaNI GCATC 2 cut(s) 321, 519
SfuI TTCGAA 1 cut(s) 857
SgrAI CRCCGGYG 1 cut(s) 446
SinI GGWCC 6 cut(s) 175, 200, 236, 473, 553, 772
SmlI CTYRAG 2 cut(s) 146, 752
SmoI CTYRAG 2 cut(s) 146, 752
SpeI ACTAGT 1 cut(s) 886
SphI GCATGC 1 cut(s) 974
Sse9I AATT 4 cut(s) 18, 628, 826, 1029
SspMI CTAG 2 cut(s) 720, 887
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 4 cut(s) 185, 196, 774, 1045
TaaI ACNGT 2 cut(s) 211, 816
TaiI ACGT 1 cut(s) 390
TaqI TCGA 3 cut(s) 36, 426, 857
TaqII GACCGA 2 cut(s) 263, 461
TasI AATT 4 cut(s) 18, 628, 826, 1029
TauI GCSGC 5 cut(s) 65, 68, 71, 495, 547
TfiI GAWTC 2 cut(s) 585, 1134
Tru1I TTAA 4 cut(s) 15, 650, 825, 1218
Tru9I TTAA 4 cut(s) 15, 650, 825, 1218
TseFI GTSAC 2 cut(s) 658, 914
TseI GCWGC 5 cut(s) 716, 734, 737, 757, 1211
Tsp45I GTSAC 2 cut(s) 658, 914
TspDTI ATGAA 4 cut(s) 17, 885, 896, 980
TspGWI ACGGA 3 cut(s) 280, 308, 1127
Tth111I GACNNNGTC 3 cut(s) 198, 386, 422
VpaK11BI GGWCC 6 cut(s) 175, 200, 236, 473, 553, 772
XapI RAATTY 1 cut(s) 1029
XceI RCATGY 2 cut(s) 945, 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 946
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 2 cut(s) 720, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.