MD13G1041100.v1.1

Aspartate-semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0003893
Physical Location & Seq
Forward (+)
2817795 .. 2821444
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1041100.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCTGTCACCCCTCTCCATCGCCATTCTAAGGTTGGCAATTTTTTTTTTTCCTTTTTTTCTTTTGTGATTTTGGATGTTTTGAAACTTTTTGAAGAACGGATCCTCAAATCGTGTCCGTTTATCGCTGCTAGTGGAGCTGGTGCTGCCGCCATGAGAGAGCTTGAGCTGCAAACTAAGGAGGTCCTGGAAGGGAAGCCGCCGACGACTAAGGTATTCTCGCGGCAGTATGCGTTTAATTTGTTTTCGCACAACTCGGCAGTTCTTTCAAATGGATACAATGAAGAGGAAATGAAACTAGTCAAGGAGACACGAAAAATATGGAGTGACAATGATGTCAAGGTAACTGCCACATGTATACGAGTTCCTGTCATGCGGGCACATACTGAGAGTATAAATCTTCAGTTTGAGAACCAACTTGATGAGAAGAGAAGGCACACTGAAAGCTTGCTGCTATGGCTTCGGGTTCTGCAACAGGTGCAGGACACAGCAAGGGAAATTCTGAAGAAATCGCCTGGACTGGAGGTTATTGATGACCGGACAGCGAATAACTTCCTACACCATTGGAGGTGTCAAACAAAGGCGATGTTGCAGTTGGATATCTGTGTCTGCGGTGATCACAAGGGAGCTGCACTTAATGTTGTTCAGATTGCCGAGATGTCGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00261 ko00270 ko00300 ko01100 ko01110 ko01120 ko01130 ko01210 ko01230 map00260 map00261 map00270 map00300 map01100 map01110 map01120 map01130 map01210 map01230
Pfam Domains
Protein Families
Semialdhyde_dh Semialdhyde_dhC

Protein Analysis

223

Amino Acids

25.36

Weight (kDa)

8.27

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Semialdhyde_dhC PF02774 42 - 139 1.7e-25 Semialdehyde dehydrogenase, dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 358
AccII CGCG 1 cut(s) 223
AciI CCGC 5 cut(s) 150, 200, 223, 376, 612
AclWI GGATC 2 cut(s) 97, 110
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 2 cut(s) 386, 524
AfiI CCNNNNNNNGG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 3 cut(s) 85, 95, 270
AhlI ACTAGT 1 cut(s) 298
AjnI CCWGG 2 cut(s) 186, 514
AluBI AGCT 5 cut(s) 140, 163, 169, 447, 629
AluI AGCT 5 cut(s) 140, 163, 169, 447, 629
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 2 cut(s) 97, 110
ApeKI GCWGC 5 cut(s) 128, 146, 169, 451, 629
ApoI RAATTY 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 551
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 626
AvaII GGWCC 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 382
BamHI GGATCC 1 cut(s) 102
BbvI GCAGC 5 cut(s) 115, 133, 156, 438, 616
BccI CCATC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 188, 516
BciVI GTATCC 1 cut(s) 269
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 1 cut(s) 302
BcuI ACTAGT 1 cut(s) 298
BfaI CTAG 2 cut(s) 132, 299
BfuI GTATCC 1 cut(s) 269
BisI GCNGC 8 cut(s) 129, 147, 150, 170, 200, 224, 452, 630
BlsI GCNGC 8 cut(s) 130, 148, 151, 171, 201, 225, 453, 631
Bme1390I CCNGG 2 cut(s) 188, 516
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 2 cut(s) 188, 516
BpmI CTGGAG 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 185
BsaWI WCCGGW 1 cut(s) 537
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 188, 516
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 525
BseSI GKGCMC 1 cut(s) 382
BseXI GCAGC 5 cut(s) 115, 133, 156, 438, 616
BsgI GTGCAG 2 cut(s) 500, 615
Bsh1236I CGCG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 538
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 2 cut(s) 102, 616
BspACI CCGC 5 cut(s) 150, 200, 223, 376, 612
BspCNI CTCAG 1 cut(s) 379
BspFNI CGCG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 104
BspPI GGATC 2 cut(s) 97, 110
BsrI ACTGG 1 cut(s) 525
BssMI GATC 2 cut(s) 102, 616
BssNAI GTATAC 1 cut(s) 359
Bst1107I GTATAC 1 cut(s) 359
Bst2UI CCWGG 2 cut(s) 188, 516
Bst6I CTCTTC 2 cut(s) 279, 422
BstAPI GCANNNNNTGC 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 378, 449
BstDEI CTNAG 4 cut(s) 30, 177, 210, 387
BstF5I GGATG 1 cut(s) 82
BstFNI CGCG 1 cut(s) 223
BstKTI GATC 2 cut(s) 105, 619
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 2 cut(s) 102, 616
BstMWI GCNNNNNNNGC 5 cut(s) 137, 146, 169, 457, 478
BstNI CCWGG 2 cut(s) 188, 516
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 186, 514
BstSLI GKGCMC 1 cut(s) 382
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 5 cut(s) 115, 133, 156, 438, 616
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BstZ17I GTATAC 1 cut(s) 359
BsuI GTATCC 1 cut(s) 269
BtgZI GCGATG 2 cut(s) 5, 599
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 438
Cac8I GCNNGC 2 cut(s) 378, 449
Cfr13I GGNCC 1 cut(s) 184
CviAII CATG 3 cut(s) 154, 354, 373
CviJI RGCY 8 cut(s) 5, 140, 163, 169, 199, 447, 460, 629
CviKI_1 RGCY 8 cut(s) 5, 140, 163, 169, 199, 447, 460, 629
DdeI CTNAG 4 cut(s) 30, 177, 210, 387
DpnI GATC 2 cut(s) 104, 618
DpnII GATC 2 cut(s) 102, 616
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
Eam1104I CTCTTC 2 cut(s) 279, 422
EarI CTCTTC 2 cut(s) 279, 422
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 386, 524
EcoO109I RGGNCCY 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 186, 514
EcoRV GATATC 1 cut(s) 601
FaeI CATG 3 cut(s) 157, 357, 376
FatI CATG 3 cut(s) 153, 353, 372
FauI CCCGC 1 cut(s) 369
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 358
Fnu4HI GCNGC 8 cut(s) 129, 147, 150, 170, 200, 224, 452, 630
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 8 cut(s) 129, 147, 150, 170, 200, 224, 452, 630
FspBI CTAG 2 cut(s) 132, 299
GluI GCNGC 8 cut(s) 129, 147, 150, 170, 200, 224, 452, 630
GsuI CTGGAG 1 cut(s) 542
HapII CCGG 1 cut(s) 538
Hin1II CATG 3 cut(s) 157, 357, 376
HindIII AAGCTT 1 cut(s) 445
HpaII CCGG 1 cut(s) 538
HphI GGTGA 1 cut(s) 626
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 2 cut(s) 504, 648
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 185, 426
HpyCH4V TGCA 5 cut(s) 172, 472, 481, 592, 632
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 146, 169, 457, 478
HpyF3I CTNAG 4 cut(s) 30, 177, 210, 387
Hsp92II CATG 3 cut(s) 157, 357, 376
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 2 cut(s) 102, 616
LmnI GCTCC 2 cut(s) 137, 626
Lsp1109I GCAGC 5 cut(s) 115, 133, 156, 438, 616
MaeI CTAG 2 cut(s) 132, 299
MaeIII GTNAC 3 cut(s) 7, 326, 343
MalI GATC 2 cut(s) 104, 618
MboI GATC 2 cut(s) 102, 616
MboII GAAGA 5 cut(s) 107, 296, 392, 439, 517
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 3 cut(s) 40, 238, 498
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 6 cut(s) 24, 116, 175, 280, 517, 561
MroXI GAANNNNTTC 1 cut(s) 551
MseI TTAA 2 cut(s) 237, 636
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 2 cut(s) 188, 516
MvaI CCWGG 2 cut(s) 188, 516
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 5 cut(s) 137, 146, 169, 457, 478
NdeII GATC 2 cut(s) 102, 616
NlaIII CATG 3 cut(s) 157, 357, 376
NlaIV GGNNCC 1 cut(s) 104
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 2 cut(s) 7, 326
NspI RCATGY 1 cut(s) 357
PciI ACATGT 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 8 cut(s) 130, 148, 151, 171, 201, 225, 453, 631
PpuMI RGGWCCY 1 cut(s) 184
PscI ACATGT 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 186, 514
PspGI CCWGG 2 cut(s) 186, 514
PspN4I GGNNCC 1 cut(s) 104
PspPI GGNCC 1 cut(s) 184
PspPPI RGGWCCY 1 cut(s) 184
PsuI RGATCY 1 cut(s) 102
SaqAI TTAA 2 cut(s) 237, 636
SatI GCNGC 8 cut(s) 129, 147, 150, 170, 200, 224, 452, 630
Sau3AI GATC 2 cut(s) 102, 616
Sau96I GGNCC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 188, 516
SduI GDGCHC 1 cut(s) 382
SinI GGWCC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SpeI ACTAGT 1 cut(s) 298
Sse9I AATT 3 cut(s) 40, 238, 498
SsiI CCGC 5 cut(s) 150, 200, 223, 376, 612
SspMI CTAG 2 cut(s) 132, 299
StyD4I CCNGG 2 cut(s) 186, 514
TaqI TCGA 1 cut(s) 662
TasI AATT 3 cut(s) 40, 238, 498
TauI GCSGC 3 cut(s) 152, 202, 226
Tru1I TTAA 2 cut(s) 237, 636
Tru9I TTAA 2 cut(s) 237, 636
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 2 cut(s) 7, 326
TseI GCWGC 5 cut(s) 128, 146, 169, 451, 629
Tsp45I GTSAC 2 cut(s) 7, 326
TspDTI ATGAA 2 cut(s) 297, 308
TspGWI ACGGA 2 cut(s) 108, 115
TspRI CASTG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 498
XceI RCATGY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 358
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 2 cut(s) 132, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.