FvH4_5g05960

Oleosin 16.4 kDa-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004052
Physical Location & Seq
Reverse (-)
3503538 .. 3504698
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g05960.t1

Sequence Viewer

Length: 609 bp
ATGGCGGATATGAGAAAACAACAACAATACGTCAGTGGTGGTCATGATCAGCAGAGTCAGTACTCCTCGGACGCTAAGAGCCTCCTTCCCCAAAACGGACCCTCCGCTTCTCAAATCATTGCCGTCGTAACACTTGTTCCGCTTGGTGGGACCCTCCTACTTCTGGCCGGCCTCACGCTGGCCGGGACTTTCATAGGGATGGCCCTCTCGACGCCGCTTTTTGTGATTTTCAGCCCGATTCTGGTGCCGGCTATTGTCACCGTCGGCTTGGCGGTGGCTGGAATATTGACCTCAGGGGCTTTCGGGATCACGGGGCTCTCTTCTTTCTCGTGGTTGGCTAACTATCTGAGACGCTCGCGTCTGCCGCAGCAGGCAATCCGAGGCGCGCAGGAGACTACGGGCCAGTTGACGCAAAAGGCGAGGGATATGGGCCAGAGTGTCACTGAGAAGGCGCAGGAAGCTGGCCAAGCTGTCTCGGGCAAGGCACAGGAGGTAGGCCAAGCTGTCCAAGGCAAGGCCGAGGAAGTGGGCCAGAATGTCAAGAGCAAGGCCCAGGACACGGGAAGGACTCACGAAGGCGGTAGAGCCCGGGATGTGAAGGTGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

203

Amino Acids

21.06

Weight (kDa)

9.69

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 37 - 149 4.3e-36 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 244
AccII CGCG 2 cut(s) 358, 386
AciI CCGC 7 cut(s) 5, 105, 140, 215, 272, 365, 579
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 3 cut(s) 165, 180, 463
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 7 cut(s) 95, 146, 163, 178, 241, 514, 559
AjnI CCWGG 1 cut(s) 552
AluBI AGCT 3 cut(s) 461, 470, 503
AluI AGCT 3 cut(s) 461, 470, 503
Alw26I GTCTC 3 cut(s) 343, 386, 478
AlwI GGATC 1 cut(s) 314
Ama87I CYCGRG 2 cut(s) 475, 588
ApeKI GCWGC 1 cut(s) 367
AspLEI GCGC 3 cut(s) 386, 388, 454
AspS9I GGNCC 7 cut(s) 98, 150, 202, 400, 430, 529, 550
AsuC2I CCSGG 3 cut(s) 184, 589, 590
AsuHPI GGTGA 1 cut(s) 250
AvaI CYCGRG 2 cut(s) 475, 588
AvaII GGWCC 2 cut(s) 98, 150
AxyI CCTNAGG 1 cut(s) 292
BalI TGGCCA 1 cut(s) 465
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 2 cut(s) 318, 589
BauI CACGAG 1 cut(s) 328
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 193
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 554
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 3 cut(s) 184, 589, 590
BcoDI GTCTC 3 cut(s) 343, 386, 478
BisI GCNGC 3 cut(s) 215, 365, 368
BlsI GCNGC 3 cut(s) 216, 366, 369
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 4 cut(s) 184, 554, 589, 590
Bme18I GGWCC 2 cut(s) 98, 150
BmeT110I CYCGRG 2 cut(s) 475, 588
BmgT120I GGNCC 7 cut(s) 98, 150, 202, 400, 430, 529, 550
BmiI GGNNCC 4 cut(s) 100, 151, 152, 246
BmrFI CCNGG 4 cut(s) 184, 554, 589, 590
BpuMI CCSGG 3 cut(s) 184, 589, 590
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 6 cut(s) 66, 379, 508, 519, 552, 588
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 7 cut(s) 95, 146, 163, 178, 241, 514, 559
Bse118I RCCGGY 2 cut(s) 167, 247
Bse1I ACTGG 1 cut(s) 403
Bse21I CCTNAGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 554
BseDI CCNNGG 6 cut(s) 66, 379, 508, 519, 552, 588
BseGI GGATG 2 cut(s) 204, 598
BseLI CCNNNNNNNGG 7 cut(s) 95, 146, 163, 178, 241, 514, 559
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 3 cut(s) 306, 338, 435
BseNI ACTGG 1 cut(s) 403
BsePI GCGCGC 1 cut(s) 384
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 379
Bsh1236I CGCG 2 cut(s) 358, 386
BshNI GGYRCC 1 cut(s) 244
BsiHKCI CYCGRG 2 cut(s) 475, 588
BsiSI CCGG 4 cut(s) 168, 183, 248, 589
BslFI GGGAC 2 cut(s) 163, 199
BslI CCNNNNNNNGG 7 cut(s) 95, 146, 163, 178, 241, 514, 559
BsmAI GTCTC 3 cut(s) 343, 386, 478
BsmBI CGTCTC 1 cut(s) 343
BsmFI GGGAC 2 cut(s) 163, 199
BsoBI CYCGRG 2 cut(s) 475, 588
Bsp1286I GDGCHC 2 cut(s) 318, 589
Bsp143I GATC 2 cut(s) 46, 306
BspACI CCGC 7 cut(s) 5, 105, 140, 215, 272, 365, 579
BspCNI CTCAG 3 cut(s) 305, 339, 436
BspFNI CGCG 2 cut(s) 358, 386
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 4 cut(s) 100, 151, 152, 246
BspPI GGATC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 117
BsrFI RCCGGY 2 cut(s) 167, 247
BsrI ACTGG 1 cut(s) 403
BssAI RCCGGY 2 cut(s) 167, 247
BssECI CCNNGG 6 cut(s) 66, 379, 508, 519, 552, 588
BssHII GCGCGC 1 cut(s) 384
BssMI GATC 2 cut(s) 46, 306
BssNI GRCGYC 1 cut(s) 212
BssSI CACGAG 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 508
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 554
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 325
BstACI GRCGYC 1 cut(s) 212
BstC8I GCNNGC 7 cut(s) 169, 180, 249, 356, 372, 386, 463
BstDEI CTNAG 4 cut(s) 75, 292, 347, 444
BstF5I GGATG 2 cut(s) 204, 598
BstFNI CGCG 2 cut(s) 358, 386
BstHHI GCGC 3 cut(s) 386, 388, 454
BstKTI GATC 2 cut(s) 49, 309
BstMAI GTCTC 3 cut(s) 343, 386, 478
BstMBI GATC 2 cut(s) 46, 306
BstMWI GCNNNNNNNGC 3 cut(s) 364, 458, 467
BstNI CCWGG 1 cut(s) 554
BstSCI CCNGG 4 cut(s) 182, 552, 587, 588
BstUI CGCG 2 cut(s) 358, 386
BstV1I GCAGC 1 cut(s) 379
Bsu36I CCTNAGG 1 cut(s) 292
BtsCI GGATG 2 cut(s) 204, 598
BtsIMutI CAGTG 2 cut(s) 40, 441
Cac8I GCNNGC 7 cut(s) 169, 180, 249, 356, 372, 386, 463
CciI TCATGA 1 cut(s) 43
CfoI GCGC 3 cut(s) 386, 388, 454
Cfr10I RCCGGY 2 cut(s) 167, 247
Cfr13I GGNCC 7 cut(s) 98, 150, 202, 400, 430, 529, 550
Cfr9I CCCGGG 1 cut(s) 588
CseI GACGC 5 cut(s) 80, 220, 347, 360, 418
Csp6I GTAC 1 cut(s) 61
CviAII CATG 1 cut(s) 44
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 4 cut(s) 75, 292, 347, 444
DpnI GATC 2 cut(s) 48, 308
DpnII GATC 2 cut(s) 46, 306
DrdI GACNNNNNNGTC 1 cut(s) 357
DseDI GACNNNNNNGTC 1 cut(s) 357
EaeI YGGCCR 3 cut(s) 165, 180, 463
Eam1104I CTCTTC 1 cut(s) 325
EarI CTCTTC 1 cut(s) 325
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 2 cut(s) 318, 589
Eco47I GGWCC 2 cut(s) 98, 150
Eco81I CCTNAGG 1 cut(s) 292
Eco88I CYCGRG 2 cut(s) 475, 588
EcoO109I RGGNCCY 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 508
EcoT38I GRGCYC 2 cut(s) 318, 589
ErhI CCWWGG 1 cut(s) 508
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 1 cut(s) 47
FaiI YATR 5 cut(s) 11, 45, 194, 428, 607
FaqI GGGAC 2 cut(s) 163, 199
FatI CATG 1 cut(s) 43
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 3 cut(s) 215, 365, 368
FokI GGATG 2 cut(s) 211, 605
FriOI GRGCYC 2 cut(s) 318, 589
FseI GGCCGGCC 1 cut(s) 171
Fsp4HI GCNGC 3 cut(s) 215, 365, 368
GlaI GCGC 3 cut(s) 385, 387, 453
GluI GCNGC 3 cut(s) 215, 365, 368
HapII CCGG 4 cut(s) 168, 183, 248, 589
HgaI GACGC 5 cut(s) 80, 220, 347, 360, 418
HhaI GCGC 3 cut(s) 386, 388, 454
Hin1I GRCGYC 1 cut(s) 212
Hin1II CATG 1 cut(s) 47
Hin6I GCGC 3 cut(s) 384, 386, 452
HinP1I GCGC 3 cut(s) 384, 386, 452
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 3 cut(s) 55, 238, 568
HpaII CCGG 4 cut(s) 168, 183, 248, 589
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 3 cut(s) 70, 348, 380
Hpy188III TCNNGA 5 cut(s) 44, 208, 304, 541, 572
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 3 cut(s) 128, 214, 266
HpyAV CCTTC 5 cut(s) 95, 442, 558, 569, 592
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 3 cut(s) 364, 458, 467
HpyF3I CTNAG 4 cut(s) 75, 292, 347, 444
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 212
Hsp92II CATG 1 cut(s) 47
HspAI GCGC 3 cut(s) 384, 386, 452
KflI GGGWCCC 1 cut(s) 150
KroI GCCGGC 2 cut(s) 167, 247
KroNI GCCGGC 2 cut(s) 169, 249
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 46, 306
Lsp1109I GCAGC 1 cut(s) 379
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 4 cut(s) 127, 256, 439, 601
MalI GATC 2 cut(s) 48, 308
MboI GATC 2 cut(s) 46, 306
MboII GAAGA 1 cut(s) 312
MhlI GDGCHC 2 cut(s) 318, 589
MlsI TGGCCA 1 cut(s) 465
MluNI TGGCCA 1 cut(s) 465
MlyI GAGTC 2 cut(s) 64, 562
Mox20I TGGCCA 1 cut(s) 465
MroNI GCCGGC 2 cut(s) 167, 247
MscI TGGCCA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 197
Msp20I TGGCCA 1 cut(s) 465
MspI CCGG 4 cut(s) 168, 183, 248, 589
MspR9I CCNGG 4 cut(s) 184, 554, 589, 590
MvaI CCWGG 1 cut(s) 554
MvnI CGCG 2 cut(s) 358, 386
MwoI GCNNNNNNNGC 3 cut(s) 364, 458, 467
NaeI GCCGGC 2 cut(s) 169, 249
NciI CCSGG 3 cut(s) 184, 589, 590
NdeII GATC 2 cut(s) 46, 306
NgoMIV GCCGGC 2 cut(s) 167, 247
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 4 cut(s) 100, 151, 152, 246
NmeAIII GCCGAG 1 cut(s) 544
NmuCI GTSAC 3 cut(s) 256, 439, 601
PagI TCATGA 1 cut(s) 43
PauI GCGCGC 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PdiI GCCGGC 2 cut(s) 169, 249
PfeI GAWTC 1 cut(s) 238
PkrI GCNGC 3 cut(s) 216, 366, 369
PleI GAGTC 2 cut(s) 63, 562
PpsI GAGTC 2 cut(s) 63, 562
PpuMI RGGWCCY 1 cut(s) 150
Psp5II RGGWCCY 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 552
PspGI CCWGG 1 cut(s) 552
PspN4I GGNNCC 4 cut(s) 100, 151, 152, 246
PspPI GGNCC 7 cut(s) 98, 150, 202, 400, 430, 529, 550
PspPPI RGGWCCY 1 cut(s) 150
PsrI GAACNNNNNNTAC 2 cut(s) 120, 152
PteI GCGCGC 1 cut(s) 384
RigI GGCCGGCC 1 cut(s) 171
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 197
SatI GCNGC 3 cut(s) 215, 365, 368
Sau3AI GATC 2 cut(s) 46, 306
Sau96I GGNCC 7 cut(s) 98, 150, 202, 400, 430, 529, 550
ScaI AGTACT 1 cut(s) 62
SchI GAGTC 2 cut(s) 64, 562
ScrFI CCNGG 4 cut(s) 184, 554, 589, 590
SduI GDGCHC 2 cut(s) 318, 589
SetI ASST 7 cut(s) 33, 293, 463, 472, 495, 505, 603
SinI GGWCC 2 cut(s) 98, 150
SmaI CCCGGG 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 197
SsiI CCGC 7 cut(s) 5, 105, 140, 215, 272, 365, 579
SspI AATATT 1 cut(s) 285
StyD4I CCNGG 4 cut(s) 182, 552, 587, 588
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 209
TatI WGTACW 1 cut(s) 60
TauI GCSGC 2 cut(s) 217, 367
TfiI GAWTC 1 cut(s) 238
TscAI CASTG 2 cut(s) 40, 448
TseFI GTSAC 3 cut(s) 256, 439, 601
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 3 cut(s) 256, 439, 601
TspDTI ATGAA 1 cut(s) 181
TspGWI ACGGA 1 cut(s) 111
TspMI CCCGGG 1 cut(s) 588
TspRI CASTG 2 cut(s) 40, 448
VpaK11BI GGWCC 2 cut(s) 98, 150
XmaI CCCGGG 1 cut(s) 588
ZrmI AGTACT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.