MD14G1174400.v1.1

Oleosin 16.4 kDa-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004052
Physical Location & Seq
Forward (+)
26826863 .. 26827489
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1174400.v1.1.491

Sequence Viewer

Length: 627 bp
ATGGCTGATCAGAACTACCGCCAGCAGCAACCACAGCACCAAGGTTACCAAAGTCACATCCAGTTCGACAACCCACAACACCAAGGTTACCAAACTCACTACCAGTATGACCAACAACAACGCTACCCCTTCGAAGTCGGGAAAAGCTTCCGTCCTCAAAATGGCTCCTCAGCTTCAAATTTTTTGGCAATTGCCACCCTGGTTCCAGTAGGGGGAACTCTGCTCTTGCTTGCAGGGCTCACACTGGCCGGGACCATCATTGGGCTGGCAGTGACCACCCCGCTTTTTGTTATCTTTAGCCCGGTTTTGGTCCCGGCTGTTGTGGTCATATTTCTGTCTGTGACGGGGATATTGACTTCGGGGGCTTTCGGGGTCACGGCCCTATCGTCTTTCTCTTGGTTAGCCCATTATTTGAGCCGGTCTCGTCTGCCCCAGACGATGGGCCAAAAGGTGCAGGAGACCACAGGATATTTGGGCCAGAAGGTGCAGGAGACGGCGGGCTACTTGGGCCAGAAGGTGGAAGAGACGGGAGACTTTGTGGGCCATAAGATGCAGGAGACTGGAGGTCAGGTGGGCCAGATGACTAAGGATGTGGGCGAGACTATCCAGCAAGGCCGAGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

209

Amino Acids

22.63

Weight (kDa)

7.02

Isoelectric Point (pI)

40.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 60 - 176 2.1e-35 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 439, 517
AciI CCGC 3 cut(s) 19, 281, 497
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 178
AfiI CCNNNNNNNGG 6 cut(s) 161, 212, 261, 307, 439, 517
AgsI TTSAA 1 cut(s) 177
AhdI GACNNNNNGTC 1 cut(s) 564
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 2 cut(s) 147, 173
AluI AGCT 2 cut(s) 147, 173
Alw26I GTCTC 7 cut(s) 426, 452, 485, 518, 525, 551, 593
AoxI GGCC 8 cut(s) 246, 378, 442, 475, 508, 541, 574, 613
ApeKI GCWGC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 178
Asp700I GAANNNNTTC 1 cut(s) 146
AspS9I GGNCC 8 cut(s) 252, 310, 379, 442, 475, 508, 541, 574
AsuC2I CCSGG 3 cut(s) 250, 302, 314
AsuII TTCGAA 1 cut(s) 132
AvaII GGWCC 2 cut(s) 252, 310
BanII GRGCYC 1 cut(s) 240
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 1 cut(s) 37
BccI CCATC 2 cut(s) 263, 433
BceAI ACGGC 2 cut(s) 393, 510
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 7
BcnI CCSGG 3 cut(s) 250, 302, 314
BcoDI GTCTC 7 cut(s) 426, 452, 485, 518, 525, 551, 593
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 4 cut(s) 200, 250, 302, 314
Bme18I GGWCC 2 cut(s) 252, 310
BmeRI GACNNNNNGTC 1 cut(s) 564
BmgT120I GGNCC 8 cut(s) 252, 310, 379, 442, 475, 508, 541, 574
BmiI GGNNCC 4 cut(s) 166, 204, 253, 312
BmrFI CCNGG 4 cut(s) 200, 250, 302, 314
BmsI GCATC 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 406, 438
BpmI CTGGAG 1 cut(s) 582
Bpu10I CCTNAGC 1 cut(s) 169
Bpu14I TTCGAA 1 cut(s) 132
BpuMI CCSGG 3 cut(s) 250, 302, 314
BsaI GGTCTC 2 cut(s) 426, 452
BsaJI CCNNGG 4 cut(s) 40, 82, 198, 616
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 555, 585
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 212, 261, 307, 439, 517
Bse118I RCCGGY 1 cut(s) 417
Bse1I ACTGG 5 cut(s) 61, 103, 206, 249, 565
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 4 cut(s) 40, 82, 198, 616
BseGI GGATG 2 cut(s) 57, 595
BseLI CCNNNNNNNGG 6 cut(s) 161, 212, 261, 307, 439, 517
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 5 cut(s) 61, 103, 206, 249, 565
BseRI GAGGAG 1 cut(s) 157
BseXI GCAGC 1 cut(s) 37
BsgI GTGCAG 2 cut(s) 473, 506
BshFI GGCC 8 cut(s) 248, 380, 444, 477, 510, 543, 576, 615
BsiSI CCGG 4 cut(s) 249, 302, 314, 418
BslFI GGGAC 2 cut(s) 265, 296
BslI CCNNNNNNNGG 6 cut(s) 161, 212, 261, 307, 439, 517
BsmAI GTCTC 7 cut(s) 426, 452, 485, 518, 525, 551, 593
BsmBI CGTCTC 2 cut(s) 485, 518
BsmFI GGGAC 2 cut(s) 265, 296
BsnI GGCC 8 cut(s) 248, 380, 444, 477, 510, 543, 576, 615
Bso31I GGTCTC 2 cut(s) 426, 452
Bsp119I TTCGAA 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 1 cut(s) 7
BspACI CCGC 3 cut(s) 19, 281, 497
BspANI GGCC 8 cut(s) 248, 380, 444, 477, 510, 543, 576, 615
BspCNI CTCAG 1 cut(s) 182
BspLI GGNNCC 4 cut(s) 166, 204, 253, 312
BspT104I TTCGAA 1 cut(s) 132
BspTNI GGTCTC 2 cut(s) 426, 452
BsrFI RCCGGY 1 cut(s) 417
BsrI ACTGG 5 cut(s) 61, 103, 206, 249, 565
BssAI RCCGGY 1 cut(s) 417
BssECI CCNNGG 4 cut(s) 40, 82, 198, 616
BssMI GATC 1 cut(s) 7
BssT1I CCWWGG 2 cut(s) 40, 82
Bst2UI CCWGG 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 516
BstBI TTCGAA 1 cut(s) 132
BstC8I GCNNGC 4 cut(s) 23, 231, 267, 499
BstDEI CTNAG 2 cut(s) 169, 585
BstEII GGTNACC 2 cut(s) 44, 86
BstF5I GGATG 2 cut(s) 57, 595
BstKTI GATC 1 cut(s) 10
BstMAI GTCTC 7 cut(s) 426, 452, 485, 518, 525, 551, 593
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 3 cut(s) 34, 235, 507
BstNI CCWGG 1 cut(s) 200
BstPI GGTNACC 2 cut(s) 44, 86
BstSCI CCNGG 4 cut(s) 198, 248, 300, 312
BstV1I GCAGC 1 cut(s) 37
BsuRI GGCC 8 cut(s) 248, 380, 444, 477, 510, 543, 576, 615
BtsCI GGATG 2 cut(s) 57, 595
BtsI GCAGTG 1 cut(s) 276
BtsIMutI CAGTG 2 cut(s) 242, 276
Cac8I GCNNGC 4 cut(s) 23, 231, 267, 499
Cfr10I RCCGGY 1 cut(s) 417
Cfr13I GGNCC 8 cut(s) 252, 310, 379, 442, 475, 508, 541, 574
DdeI CTNAG 2 cut(s) 169, 585
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
DriI GACNNNNNGTC 1 cut(s) 564
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 2 cut(s) 40, 82
Eco24I GRGCYC 1 cut(s) 240
Eco31I GGTCTC 2 cut(s) 426, 452
Eco47I GGWCC 2 cut(s) 252, 310
Eco91I GGTNACC 2 cut(s) 44, 86
EcoO65I GGTNACC 2 cut(s) 44, 86
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 40, 82
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 2 cut(s) 40, 82
Esp3I CGTCTC 2 cut(s) 485, 518
FaiI YATR 3 cut(s) 108, 329, 546
FaqI GGGAC 2 cut(s) 265, 296
FauI CCCGC 2 cut(s) 288, 490
FbaI TGATCA 1 cut(s) 7
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 2 cut(s) 44, 602
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 26
GluI GCNGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 8 cut(s) 248, 380, 444, 477, 510, 543, 576, 615
HapII CCGG 4 cut(s) 249, 302, 314, 418
HindIII AAGCTT 1 cut(s) 145
HpaII CCGG 4 cut(s) 249, 302, 314, 418
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 139, 475, 508
HpyCH4V TGCA 4 cut(s) 233, 454, 487, 553
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 235, 507
HpyF3I CTNAG 2 cut(s) 169, 585
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 170
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 1 cut(s) 540
MaeIII GTNAC 6 cut(s) 44, 53, 86, 271, 340, 373
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MboII GAAGA 1 cut(s) 533
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 2 cut(s) 178, 189
MnlI CCTC 4 cut(s) 165, 178, 557, 611
MroXI GAANNNNTTC 1 cut(s) 146
MspI CCGG 4 cut(s) 249, 302, 314, 418
MspR9I CCNGG 4 cut(s) 200, 250, 302, 314
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 3 cut(s) 34, 235, 507
NciI CCSGG 3 cut(s) 250, 302, 314
NdeII GATC 1 cut(s) 7
NlaIV GGNNCC 4 cut(s) 166, 204, 253, 312
NmuCI GTSAC 4 cut(s) 53, 271, 340, 373
NspV TTCGAA 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 146
PflMI CCANNNNNTGG 2 cut(s) 439, 517
PkrI GCNGC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 198
PspEI GGTNACC 2 cut(s) 44, 86
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 4 cut(s) 166, 204, 253, 312
PspPI GGNCC 8 cut(s) 252, 310, 379, 442, 475, 508, 541, 574
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 8 cut(s) 252, 310, 379, 442, 475, 508, 541, 574
ScrFI CCNGG 4 cut(s) 200, 250, 302, 314
SduI GDGCHC 1 cut(s) 240
SetI ASST 9 cut(s) 46, 88, 149, 175, 453, 486, 519, 568, 573
SfaNI GCATC 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 132
SinI GGWCC 2 cut(s) 252, 310
Sse9I AATT 2 cut(s) 178, 189
SsiI CCGC 3 cut(s) 19, 281, 497
StyD4I CCNGG 4 cut(s) 198, 248, 300, 312
StyI CCWWGG 2 cut(s) 40, 82
TaqI TCGA 2 cut(s) 66, 132
TasI AATT 2 cut(s) 178, 189
TscAI CASTG 2 cut(s) 249, 276
TseFI GTSAC 4 cut(s) 53, 271, 340, 373
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 4 cut(s) 53, 271, 340, 373
TspGWI ACGGA 1 cut(s) 140
TspRI CASTG 2 cut(s) 249, 276
Van91I CCANNNNNTGG 2 cut(s) 439, 517
VpaK11BI GGWCC 2 cut(s) 252, 310
XapI RAATTY 1 cut(s) 178
XcmI CCANNNNNNNNNTGG 2 cut(s) 262, 469
XmnI GAANNNNTTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.