FvH4_6g51310

Oleosin 18.2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004052
Physical Location & Seq
Forward (+)
38047522 .. 38048031
510 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g51310.t1

Sequence Viewer

Length: 510 bp
ATGGCTGACCGTCCTCAGCCACACCAGATCCAAGTCCGCCCGGCTCAGCCACGATTTGACGTTGGAGATTACAAGGGCATGCAGACTCAGCAGCATCATGGCCCTTCCACCGGCAAGATCCTCGCGGTGATAACTCTCCTTCCAGTCGGTGGAACCCTCCTCGGTTTGGCCGGACTCACATTCATGGGCACCATCATCGGCCTTCTTTGTGCTGCGCCGCTCTTCCTTGTCTTCAGTCCGGTTCTTGTTCCGGCTGCCATTGTCTTAGGCCTTGCCGTCACGGCGTTCATCACGTCGGGGGCTTTTGGTCTCACTGGGCTCTCATCGCTGTCTTGGGTCACAAACTACCTCCGTAGGGCTACTGGGACAGTGCCCGAGCAGCTTGACTCGGCGAAAAGGCGCATGGCGGATATGGGTGAGTATTTGGGACAGAAGACGAAGGAGGTGGGTCAGGACATCCAGCAGAAGGCGCAGGAGACGAAGAGGACAACCGGACATGAGGTCCGTTGA

Protein Analysis

170

Amino Acids

18.11

Weight (kDa)

9.66

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 39 - 150 3.6e-41 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 220
AccII CGCG 1 cut(s) 125
AciI CCGC 4 cut(s) 37, 125, 218, 407
AclWI GGATC 2 cut(s) 22, 112
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 217
AfiI CCNNNNNNNGG 5 cut(s) 110, 149, 166, 355, 466
AhdI GACNNNNNGTC 1 cut(s) 500
AjiI CACGTC 1 cut(s) 294
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Alw26I GTCTC 2 cut(s) 314, 470
AlwI GGATC 2 cut(s) 22, 112
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 4 cut(s) 100, 168, 199, 268
ApeKI GCWGC 4 cut(s) 91, 212, 254, 379
AspLEI GCGC 3 cut(s) 217, 402, 472
AspS9I GGNCC 2 cut(s) 101, 502
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 139, 428
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 2 cut(s) 191, 375
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 321
BbsI GAAGAC 2 cut(s) 223, 440
BbvCI CCTCAGC 1 cut(s) 15
BbvI GCAGC 4 cut(s) 103, 199, 241, 391
BccI CCATC 1 cut(s) 200
BceAI ACGGC 2 cut(s) 260, 297
BcgI CGANNNNNNTGC 4 cut(s) 103, 137, 178, 212
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 314, 470
BglI GCCNNNNNGGC 1 cut(s) 281
BisI GCNGC 5 cut(s) 92, 213, 218, 255, 380
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 5 cut(s) 93, 214, 219, 256, 381
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 502
BmeRI GACNNNNNGTC 1 cut(s) 500
BmeT110I CYCGRG 1 cut(s) 374
BmgBI CACGTC 1 cut(s) 294
BmgT120I GGNCC 2 cut(s) 101, 502
BmiI GGNNCC 2 cut(s) 154, 190
BmrFI CCNGG 1 cut(s) 41
BmrI ACTGGG 2 cut(s) 324, 372
BmsI GCATC 1 cut(s) 103
BmuI ACTGGG 2 cut(s) 324, 372
BpiI GAAGAC 2 cut(s) 223, 440
Bpu10I CCTNAGC 1 cut(s) 15
Bpu1102I GCTNAGC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 2 cut(s) 238, 491
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 149, 166, 355, 466
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 143, 319, 367
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 5 cut(s) 110, 149, 166, 355, 466
BseMII CTCAG 3 cut(s) 29, 59, 101
BseNI ACTGG 3 cut(s) 143, 319, 367
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 2 cut(s) 191, 375
BseXI GCAGC 4 cut(s) 103, 199, 241, 391
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 4 cut(s) 102, 170, 201, 270
BshNI GGYRCC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 6 cut(s) 41, 111, 171, 239, 251, 492
BslFI GGGAC 2 cut(s) 379, 441
BslI CCNNNNNNNGG 5 cut(s) 110, 149, 166, 355, 466
BsmAI GTCTC 2 cut(s) 314, 470
BsmBI CGTCTC 1 cut(s) 470
BsmFI GGGAC 2 cut(s) 379, 441
BsnI GGCC 4 cut(s) 102, 170, 201, 270
Bso31I GGTCTC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 191, 321, 375
Bsp143I GATC 2 cut(s) 27, 117
Bsp1720I GCTNAGC 1 cut(s) 45
BspACI CCGC 4 cut(s) 37, 125, 218, 407
BspANI GGCC 4 cut(s) 102, 170, 201, 270
BspCNI CTCAG 3 cut(s) 28, 58, 100
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 2 cut(s) 154, 190
BspPI GGATC 2 cut(s) 22, 112
BspQI GCTCTTC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 3 cut(s) 143, 319, 367
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 27, 117
Bst4CI ACNGT 2 cut(s) 11, 370
Bst6I CTCTTC 2 cut(s) 227, 476
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 4 cut(s) 15, 45, 87, 265
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 3 cut(s) 217, 402, 472
BstKTI GATC 2 cut(s) 30, 120
BstMAI GTCTC 2 cut(s) 314, 470
BstMBI GATC 2 cut(s) 27, 117
BstMWI GCNNNNNNNGC 5 cut(s) 88, 281, 325, 379, 469
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 39
BstSLI GKGCMC 2 cut(s) 191, 375
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 4 cut(s) 103, 199, 241, 391
BstV2I GAAGAC 2 cut(s) 223, 440
BstX2I RGATCY 2 cut(s) 27, 117
BstYI RGATCY 2 cut(s) 27, 117
BsuRI GGCC 4 cut(s) 102, 170, 201, 270
BtgZI GCGATG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 294
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 2 cut(s) 312, 375
Cac8I GCNNGC 1 cut(s) 80
CfoI GCGC 3 cut(s) 217, 402, 472
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 101, 502
CviAII CATG 5 cut(s) 79, 98, 184, 403, 497
DdeI CTNAG 4 cut(s) 15, 45, 87, 265
DpnI GATC 2 cut(s) 29, 119
DpnII GATC 2 cut(s) 27, 117
DriI GACNNNNNGTC 1 cut(s) 500
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 227, 476
Eam1105I GACNNNNNGTC 1 cut(s) 500
EarI CTCTTC 2 cut(s) 227, 476
EciI GGCGGA 2 cut(s) 26, 422
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 502
Eco57I CTGAAG 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 5 cut(s) 82, 101, 187, 406, 500
FaiI YATR 6 cut(s) 80, 99, 185, 404, 413, 498
FaqI GGGAC 2 cut(s) 379, 441
FatI CATG 5 cut(s) 78, 97, 183, 402, 496
Fnu4HI GCNGC 5 cut(s) 92, 213, 218, 255, 380
FokI GGATG 1 cut(s) 443
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 5 cut(s) 92, 213, 218, 255, 380
GlaI GCGC 3 cut(s) 216, 401, 471
GluI GCNGC 5 cut(s) 92, 213, 218, 255, 380
HaeIII GGCC 4 cut(s) 102, 170, 201, 270
HapII CCGG 6 cut(s) 41, 111, 171, 239, 251, 492
HhaI GCGC 3 cut(s) 217, 402, 472
Hin1II CATG 5 cut(s) 82, 101, 187, 406, 500
Hin6I GCGC 3 cut(s) 215, 400, 470
HinP1I GCGC 3 cut(s) 215, 400, 470
HinfI GANTC 3 cut(s) 85, 174, 386
HpaII CCGG 6 cut(s) 41, 111, 171, 239, 251, 492
HphI GGTGA 2 cut(s) 139, 428
Hpy188III TCNNGA 1 cut(s) 452
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 5 cut(s) 114, 149, 212, 433, 460
HpyCH4III ACNGT 2 cut(s) 11, 370
HpyCH4IV ACGT 2 cut(s) 60, 293
HpyCH4V TGCA 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 281, 325, 379, 469
HpyF3I CTNAG 4 cut(s) 15, 45, 87, 265
HpySE526I ACGT 2 cut(s) 60, 293
Hsp92II CATG 5 cut(s) 82, 101, 187, 406, 500
HspAI GCGC 3 cut(s) 215, 400, 470
Kzo9I GATC 2 cut(s) 27, 117
LguI GCTCTTC 1 cut(s) 227
Lsp1109I GCAGC 4 cut(s) 103, 199, 241, 391
LweI GCATC 1 cut(s) 103
MaeII ACGT 2 cut(s) 60, 293
MaeIII GTNAC 2 cut(s) 277, 337
MalI GATC 2 cut(s) 29, 119
MbiI CCGCTC 1 cut(s) 220
MboI GATC 2 cut(s) 27, 117
MboII GAAGA 4 cut(s) 214, 223, 445, 493
MflI RGATCY 2 cut(s) 27, 117
MhlI GDGCHC 3 cut(s) 191, 321, 375
MlyI GAGTC 3 cut(s) 79, 168, 380
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 8 cut(s) 24, 131, 167, 170, 359, 436, 477, 493
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 6 cut(s) 41, 111, 171, 239, 251, 492
MspR9I CCNGG 1 cut(s) 41
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 88, 281, 325, 379, 469
NciI CCSGG 1 cut(s) 41
NdeII GATC 2 cut(s) 27, 117
NlaIII CATG 5 cut(s) 82, 101, 187, 406, 500
NlaIV GGNNCC 2 cut(s) 154, 190
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 2 cut(s) 277, 337
NspI RCATGY 1 cut(s) 82
PaeI GCATGC 1 cut(s) 82
PceI AGGCCT 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 227
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 5 cut(s) 93, 214, 219, 256, 381
PleI GAGTC 3 cut(s) 79, 168, 380
PpsI GAGTC 3 cut(s) 79, 168, 380
PspN4I GGNNCC 2 cut(s) 154, 190
PspPI GGNCC 2 cut(s) 101, 502
PsuI RGATCY 2 cut(s) 27, 117
RseI CAYNNNNRTG 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 227
SatI GCNGC 5 cut(s) 92, 213, 218, 255, 380
Sau3AI GATC 2 cut(s) 27, 117
Sau96I GGNCC 2 cut(s) 101, 502
SchI GAGTC 3 cut(s) 79, 168, 380
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 3 cut(s) 191, 321, 375
SetI ASST 6 cut(s) 63, 296, 351, 384, 447, 504
SfaNI GCATC 1 cut(s) 103
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 182
SphI GCATGC 1 cut(s) 82
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 4 cut(s) 37, 125, 218, 407
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 11, 370
TaiI ACGT 2 cut(s) 63, 296
TauI GCSGC 1 cut(s) 220
TscAI CASTG 2 cut(s) 319, 375
TseFI GTSAC 2 cut(s) 277, 337
TseI GCWGC 4 cut(s) 91, 212, 254, 379
Tsp45I GTSAC 2 cut(s) 277, 337
TspDTI ATGAA 2 cut(s) 172, 277
TspGWI ACGGA 2 cut(s) 341, 494
TspRI CASTG 2 cut(s) 319, 375
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 502
XceI RCATGY 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.