FvH4_5g15580

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004857
Physical Location & Seq
Reverse (-)
8784747 .. 8786880
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15580.t1

Sequence Viewer

Length: 1344 bp
ATGCGACAGTCACCAACTGGCGCCAAGCTACCACCGGGCGTCATGGGATTTCCTTTCATCGGTGAAATGCTCACTTTCCTTTGGTATTTCAAAGTACTTCGTCGCCCGGATGATTTCATCAACGCTAAGAGGACTAAGTATGGCGATGGAGTAGGCATGTATAGAACGCACCTCTTCGGATCACCATCCATCATAACATGCTTCCCTGCGGTCAACAAATATGTATTCCAATCTGATGACATATTCACCTCAGTATGGCCTAATGTTGATCTTATGGGTCCAACTTCACTGGTATCAGTTAGTGGAAAGTCTCATACTAGGGTTAGAAGCTACGTCACCAATGTAATAAACCGGCCGGACGCTCTCCGGCGCATTGCCCTCCTTGTTCAACCTCGCATCATCGCTGCACTTGAGTCGTGGGCGCAAAAGGGTAGAATCAAAGCTTTTGATGAAGCTAACAAGTTATCATTTGAGAATATCGGACAACTATTCATAAGTATGGATCCAGGGCCTCTCTTAGATACCATCGATAACTTGTTTACTGGCTTGCTGAAAGGAGTTAGAGCTCAACCCATCAACATTCCCGGATTCGCTTATCATCATGCTCGTCAGTGCAGACAAAAGCTTGATGCTATTTTCAGGATGGAGCTGGAAAAGAAGAAGAACCAAAGTGAAGTTTTGACAAATGATGTAATGGATGGGTTGATGCAAATCAAAGATGATGCAGGTAATCAGTTAAGTGAGGAAGAGGTGACAGATAACATTGTGAGCCTTGTTATTGCTGGATATGCCTCAACTTCCCTTGCATCAATGTGGGCTATCTATTTTCTTGCCAAAAACCCCGATGTTCTCCAAAAGCTACGGGAGGAGAATATGAGCTTAAGTGAGAAGAAGAAAGGGGATCTCATTACTAGCGATGATATTTCAAAAATGAAATACACAAACAAGGTTGTGGAAGAGACGATAAGAATGGCAAACCTTGCTGCGTTCATTTTCCGTTTGGCTACTAAGGAAGCCGAGTACAAAGGTTATAGGATACCAAAGGGTTGGAAAGTGATTCTTTGGATTCGATATATCCACACAAACTCGGAAAATTTCGATAATCCTATGTGTTTTAATCCAGATAGATGGGATCAACCAGCTAAACCAGGAACATACCAAGTTTTTGGTGGCGGACCAAGAGTTTGTGCAGGAAATATGCTTGTTAGGATACAACTTGCAATCTTATTGCATCATTTGTCTGTTGGATACAAGTGGAAGTTGCTGAATCCAGATGTAGGGATGGTTTATCTTTCACATCCGAAGCCAATTGACAATGTTGATATCACTATTAGCAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

448

Amino Acids

50.6

Weight (kDa)

9.27

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 16 - 421 1.3e-51 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 716
AccB1I GGYRCC 1 cut(s) 20
AciI CCGC 2 cut(s) 209, 1173
AclWI GGATC 5 cut(s) 187, 497, 510, 909, 1140
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 2 cut(s) 1093, 1338
AcyI GRCGYC 2 cut(s) 21, 39
AfaI GTAC 2 cut(s) 96, 1022
AfiI CCNNNNNNNGG 3 cut(s) 59, 255, 1277
AflII CTTAAG 1 cut(s) 880
AgsI TTSAA 3 cut(s) 91, 389, 927
AjnI CCWGG 2 cut(s) 505, 1147
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 2 cut(s) 315, 953
AlwI GGATC 5 cut(s) 187, 497, 510, 909, 1140
AoxI GGCC 3 cut(s) 257, 353, 509
ApeKI GCWGC 2 cut(s) 404, 983
ApoI RAATTY 2 cut(s) 1093, 1338
ArsI GACNNNNNNTTYG 2 cut(s) 1167, 1199
AspLEI GCGC 3 cut(s) 23, 372, 424
AspS9I GGNCC 3 cut(s) 278, 509, 1175
AsuC2I CCSGG 3 cut(s) 36, 107, 585
AsuHPI GGTGA 6 cut(s) 3, 74, 174, 238, 328, 763
AvaII GGWCC 2 cut(s) 278, 1175
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 568
BbvI GCAGC 2 cut(s) 391, 970
BccI CCATC 9 cut(s) 140, 193, 197, 533, 581, 637, 692, 1122, 1276
BcgI CGANNNNNNTGC 2 cut(s) 396, 430
BciT130I CCWGG 2 cut(s) 507, 1149
BciVI GTATCC 3 cut(s) 1029, 1203, 1241
BcnI CCSGG 3 cut(s) 36, 107, 585
BcoDI GTCTC 2 cut(s) 315, 953
BfaI CTAG 2 cut(s) 318, 912
BfoI RGCGCY 1 cut(s) 24
BfrI CTTAAG 1 cut(s) 880
BfuAI ACCTGC 1 cut(s) 716
BfuI GTATCC 3 cut(s) 1029, 1203, 1241
BisI GCNGC 2 cut(s) 405, 984
BlsI GCNGC 2 cut(s) 406, 985
BmcAI AGTACT 1 cut(s) 96
Bme1390I CCNGG 5 cut(s) 36, 107, 507, 585, 1149
Bme18I GGWCC 2 cut(s) 278, 1175
BmgT120I GGNCC 3 cut(s) 278, 509, 1175
BmiI GGNNCC 3 cut(s) 22, 279, 504
BmrFI CCNGG 5 cut(s) 36, 107, 507, 585, 1149
BmsI GCATC 6 cut(s) 405, 619, 696, 712, 815, 1240
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 3 cut(s) 36, 107, 585
Bsa29I ATCGAT 1 cut(s) 528
BsaBI GATNNNNATC 2 cut(s) 184, 710
BsaHI GRCGYC 2 cut(s) 21, 39
BsaJI CCNNGG 1 cut(s) 506
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 255, 1277
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 3 cut(s) 22, 294, 547
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 2 cut(s) 184, 710
BseBI CCWGG 2 cut(s) 507, 1149
BseCI ATCGAT 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 6 cut(s) 115, 185, 648, 703, 1287, 1297
BseJI GATNNNNATC 2 cut(s) 184, 710
BseLI CCNNNNNNNGG 3 cut(s) 59, 255, 1277
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 3 cut(s) 22, 294, 547
BseRI GAGGAG 1 cut(s) 881
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 2 cut(s) 391, 970
BsgI GTGCAG 3 cut(s) 390, 634, 1209
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 3 cut(s) 259, 355, 511
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 568
BsiSI CCGG 6 cut(s) 35, 107, 352, 356, 367, 585
BslI CCNNNNNNNGG 3 cut(s) 59, 255, 1277
BsmAI GTCTC 2 cut(s) 315, 953
BsmBI CGTCTC 1 cut(s) 953
BsnI GGCC 3 cut(s) 259, 355, 511
Bsp1286I GDGCHC 1 cut(s) 568
Bsp143I GATC 5 cut(s) 179, 268, 502, 901, 1132
BspACI CCGC 2 cut(s) 209, 1173
BspANI GGCC 3 cut(s) 259, 355, 511
BspCNI CTCAG 1 cut(s) 263
BspDI ATCGAT 1 cut(s) 528
BspLI GGNNCC 3 cut(s) 22, 279, 504
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 5 cut(s) 187, 497, 510, 909, 1140
BspT107I GGYRCC 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 880
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 3 cut(s) 22, 294, 547
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 5 cut(s) 179, 268, 502, 901, 1132
BssNI GRCGYC 2 cut(s) 21, 39
Bst2UI CCWGG 2 cut(s) 507, 1149
Bst4CI ACNGT 1 cut(s) 9
Bst6I CTCTTC 3 cut(s) 179, 741, 951
BstACI GRCGYC 2 cut(s) 21, 39
BstAFI CTTAAG 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 980
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 5 cut(s) 126, 135, 250, 517, 1008
BstF5I GGATG 6 cut(s) 115, 185, 648, 703, 1287, 1297
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 3 cut(s) 23, 372, 424
BstKTI GATC 5 cut(s) 182, 271, 505, 904, 1135
BstMAI GTCTC 2 cut(s) 315, 953
BstMBI GATC 5 cut(s) 179, 268, 502, 901, 1132
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 2 cut(s) 788, 980
BstNI CCWGG 2 cut(s) 507, 1149
BstNSI RCATGY 2 cut(s) 160, 201
BstSCI CCNGG 5 cut(s) 34, 105, 505, 583, 1147
BstV1I GCAGC 2 cut(s) 391, 970
BstX2I RGATCY 2 cut(s) 502, 901
BstXI CCANNNNNNTGG 3 cut(s) 1047, 1128, 1166
BstYI RGATCY 2 cut(s) 502, 901
BstZI CGGCCG 1 cut(s) 353
Bsu15I ATCGAT 1 cut(s) 528
BsuI GTATCC 3 cut(s) 1029, 1203, 1241
BsuRI GGCC 3 cut(s) 259, 355, 511
BsuTUI ATCGAT 1 cut(s) 528
BtgZI GCGATG 3 cut(s) 159, 385, 930
BtsCI GGATG 6 cut(s) 115, 185, 648, 703, 1287, 1297
BtsIMutI CAGTG 2 cut(s) 287, 617
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 1 cut(s) 548
CfoI GCGC 3 cut(s) 23, 372, 424
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 3 cut(s) 278, 509, 1175
ClaI ATCGAT 1 cut(s) 528
CseI GACGC 2 cut(s) 28, 368
Csp6I GTAC 2 cut(s) 95, 1021
CviAII CATG 4 cut(s) 43, 157, 198, 602
CviQI GTAC 2 cut(s) 95, 1021
DdeI CTNAG 5 cut(s) 126, 135, 250, 517, 1008
DinI GGCGCC 1 cut(s) 22
DpnI GATC 5 cut(s) 181, 270, 504, 903, 1134
DpnII GATC 5 cut(s) 179, 268, 502, 901, 1132
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
Eam1104I CTCTTC 3 cut(s) 179, 741, 951
EarI CTCTTC 3 cut(s) 179, 741, 951
EciI GGCGGA 1 cut(s) 1188
Ecl136II GAGCTC 1 cut(s) 566
EclXI CGGCCG 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 1324
Eco47I GGWCC 2 cut(s) 278, 1175
Eco52I CGGCCG 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 505, 1147
EcoRV GATATC 1 cut(s) 1324
EcoT38I GRGCYC 1 cut(s) 568
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
Esp3I CGTCTC 1 cut(s) 953
FaeI CATG 4 cut(s) 46, 160, 201, 605
FalI AAGNNNNNCTT 2 cut(s) 1044, 1076
FatI CATG 4 cut(s) 42, 156, 197, 601
Fnu4HI GCNGC 2 cut(s) 405, 984
FokI GGATG 6 cut(s) 122, 172, 655, 710, 1284, 1294
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 2 cut(s) 405, 984
FspBI CTAG 2 cut(s) 318, 912
GlaI GCGC 3 cut(s) 22, 371, 423
GluI GCNGC 2 cut(s) 405, 984
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 3 cut(s) 259, 355, 511
HapII CCGG 6 cut(s) 35, 107, 352, 356, 367, 585
HgaI GACGC 2 cut(s) 28, 368
HhaI GCGC 3 cut(s) 23, 372, 424
Hin1I GRCGYC 2 cut(s) 21, 39
Hin1II CATG 4 cut(s) 46, 160, 201, 605
Hin6I GCGC 3 cut(s) 21, 370, 422
HinP1I GCGC 3 cut(s) 21, 370, 422
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 2 cut(s) 441, 623
HinfI GANTC 6 cut(s) 413, 435, 588, 1057, 1066, 1267
HpaII CCGG 6 cut(s) 35, 107, 352, 356, 367, 585
HphI GGTGA 6 cut(s) 3, 74, 174, 238, 328, 763
Hpy166II GTNNAC 2 cut(s) 214, 540
Hpy188I TCNGA 5 cut(s) 179, 235, 482, 1090, 1302
Hpy188III TCNNGA 3 cut(s) 640, 1121, 1271
Hpy8I GTNNAC 2 cut(s) 214, 540
Hpy99I CGWCG 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 8 cut(s) 407, 615, 709, 725, 806, 1190, 1220, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 788, 980
HpyF3I CTNAG 5 cut(s) 126, 135, 250, 517, 1008
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 2 cut(s) 21, 39
Hsp92II CATG 4 cut(s) 46, 160, 201, 605
HspAI GCGC 3 cut(s) 21, 370, 422
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 179, 268, 502, 901, 1132
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 2 cut(s) 391, 970
LweI GCATC 6 cut(s) 405, 619, 696, 712, 815, 1240
MaeI CTAG 2 cut(s) 318, 912
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 3 cut(s) 9, 334, 751
MalI GATC 5 cut(s) 181, 270, 504, 903, 1134
MboI GATC 5 cut(s) 179, 268, 502, 901, 1132
MboII GAAGA 7 cut(s) 166, 670, 673, 758, 901, 904, 968
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 2 cut(s) 502, 901
MhlI GDGCHC 1 cut(s) 568
MluCI AATT 3 cut(s) 1093, 1308, 1338
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 422
MmeI TCCRAC 3 cut(s) 305, 1028, 1225
MseI TTAA 4 cut(s) 737, 881, 1116, 1342
MslI CAYNNNNRTG 2 cut(s) 497, 811
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 6 cut(s) 35, 107, 352, 356, 367, 585
MspR9I CCNGG 5 cut(s) 36, 107, 507, 585, 1149
MunI CAATTG 1 cut(s) 1308
MvaI CCWGG 2 cut(s) 507, 1149
MwoI GCNNNNNNNGC 2 cut(s) 788, 980
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 3 cut(s) 36, 107, 585
NdeII GATC 5 cut(s) 179, 268, 502, 901, 1132
NlaIII CATG 4 cut(s) 46, 160, 201, 605
NlaIV GGNNCC 3 cut(s) 22, 279, 504
NmeAIII GCCGAG 1 cut(s) 1042
NmuCI GTSAC 3 cut(s) 9, 334, 751
NspI RCATGY 2 cut(s) 160, 201
PfeI GAWTC 5 cut(s) 435, 588, 1057, 1066, 1267
PfoI TCCNGGA 1 cut(s) 583
PkrI GCNGC 2 cut(s) 406, 985
PleI GAGTC 1 cut(s) 421
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 421
Psp124BI GAGCTC 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 505, 1147
PspGI CCWGG 2 cut(s) 505, 1147
PspN4I GGNNCC 3 cut(s) 22, 279, 504
PspPI GGNCC 3 cut(s) 278, 509, 1175
PsuI RGATCY 2 cut(s) 502, 901
RsaI GTAC 2 cut(s) 96, 1022
RsaNI GTAC 2 cut(s) 95, 1021
RseI CAYNNNNRTG 2 cut(s) 497, 811
SacI GAGCTC 1 cut(s) 568
SaqAI TTAA 4 cut(s) 737, 881, 1116, 1342
SatI GCNGC 2 cut(s) 405, 984
Sau3AI GATC 5 cut(s) 179, 268, 502, 901, 1132
Sau96I GGNCC 3 cut(s) 278, 509, 1175
ScaI AGTACT 1 cut(s) 96
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 5 cut(s) 36, 107, 507, 585, 1149
SduI GDGCHC 1 cut(s) 568
SfaNI GCATC 6 cut(s) 405, 619, 696, 712, 815, 1240
SfoI GGCGCC 1 cut(s) 22
SinI GGWCC 2 cut(s) 278, 1175
SmiMI CAYNNNNRTG 2 cut(s) 497, 811
SmlI CTYRAG 2 cut(s) 410, 880
SmoI CTYRAG 2 cut(s) 410, 880
Sse9I AATT 3 cut(s) 1093, 1308, 1338
SsiI CCGC 2 cut(s) 209, 1173
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 2 cut(s) 318, 912
SstI GAGCTC 1 cut(s) 568
StyD4I CCNGG 5 cut(s) 34, 105, 505, 583, 1147
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 1 cut(s) 336
TaqI TCGA 3 cut(s) 528, 1069, 1098
TasI AATT 3 cut(s) 1093, 1308, 1338
TatI WGTACW 2 cut(s) 94, 1020
TfiI GAWTC 5 cut(s) 435, 588, 1057, 1066, 1267
Tru1I TTAA 4 cut(s) 737, 881, 1116, 1342
Tru9I TTAA 4 cut(s) 737, 881, 1116, 1342
TscAI CASTG 2 cut(s) 294, 617
TseFI GTSAC 3 cut(s) 9, 334, 751
TseI GCWGC 2 cut(s) 404, 983
Tsp45I GTSAC 3 cut(s) 9, 334, 751
TspDTI ATGAA 6 cut(s) 46, 106, 465, 481, 947, 979
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 2 cut(s) 294, 617
Vha464I CTTAAG 1 cut(s) 880
VpaK11BI GGWCC 2 cut(s) 278, 1175
XapI RAATTY 2 cut(s) 1093, 1338
XceI RCATGY 2 cut(s) 160, 201
XcmI CCANNNNNNNNNTGG 1 cut(s) 1166
XspI CTAG 2 cut(s) 318, 912
ZrmI AGTACT 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.