Rmu_sc0005690.1_g000006

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005690.1
OG0004857
Physical Location & Seq
Forward (+)
26734 .. 27030
297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005690.1_g000006.1.cds

Sequence Viewer

Length: 297 bp
atgtatagaacgcacctcttcggctcgccgtcgatcataacatgtttcccggcagtgaacaaatttatattccaatcggaggacaccttcaaactagactggcctgacgttgatattgtaggttctagttccttggtaacagttcaaggaagctcccatgcagggcttagaagctatgtcgtaaatgtcatcaacaaacccgacgctctccgccgcattgcgcaactagttcagccgcgaatgttggccgcatttagtcatgggctctcaaaggtagaattgggctctcaaaggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

98

Amino Acids

10.85

Weight (kDa)

9.43

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 222
AccII CGCG 1 cut(s) 238
AciI CCGC 4 cut(s) 211, 214, 236, 249
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 62
AfiI CCNNNNNNNGG 2 cut(s) 79, 162
AflIII ACRYGT 1 cut(s) 41
AgsI TTSAA 2 cut(s) 91, 146
AhlI ACTAGT 1 cut(s) 226
AluBI AGCT 2 cut(s) 153, 174
AluI AGCT 2 cut(s) 153, 174
AoxI GGCC 2 cut(s) 101, 246
ApoI RAATTY 1 cut(s) 62
AspLEI GCGC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 50
BanII GRGCYC 2 cut(s) 267, 287
BceAI ACGGC 1 cut(s) 13
BcnI CCSGG 1 cut(s) 50
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 3 cut(s) 95, 126, 227
BisI GCNGC 3 cut(s) 214, 236, 249
BlsI GCNGC 3 cut(s) 215, 237, 250
Bme1390I CCNGG 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 50
BsaJI CCNNGG 1 cut(s) 132
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 162
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 79, 162
BseMI GCAATG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 2 cut(s) 103, 248
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 79, 162
BsnI GGCC 2 cut(s) 103, 248
Bsp1286I GDGCHC 2 cut(s) 267, 287
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 4 cut(s) 211, 214, 236, 249
BspANI GGCC 2 cut(s) 103, 248
BspFNI CGCG 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 36
BstMBI GATC 1 cut(s) 33
BstNSI RCATGY 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 48
BstUI CGCG 1 cut(s) 238
BsuRI GGCC 2 cut(s) 103, 248
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 223
CseI GACGC 1 cut(s) 212
CviAII CATG 3 cut(s) 42, 158, 260
CviJI RGCY 9 cut(s) 24, 103, 153, 166, 174, 235, 248, 265, 285
CviKI_1 RGCY 9 cut(s) 24, 103, 153, 166, 174, 235, 248, 265, 285
DdeI CTNAG 1 cut(s) 167
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 246
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 2 cut(s) 267, 287
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 2 cut(s) 267, 287
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 3 cut(s) 45, 161, 263
FaiI YATR 7 cut(s) 6, 38, 43, 68, 159, 177, 261
FatI CATG 3 cut(s) 41, 157, 259
Fnu4HI GCNGC 3 cut(s) 214, 236, 249
FriOI GRGCYC 2 cut(s) 267, 287
Fsp4HI GCNGC 3 cut(s) 214, 236, 249
FspBI CTAG 3 cut(s) 95, 126, 227
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 1 cut(s) 222
GluI GCNGC 3 cut(s) 214, 236, 249
HaeIII GGCC 2 cut(s) 103, 248
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 223
Hin1II CATG 3 cut(s) 45, 161, 263
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HpaII CCGG 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 79
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 2 cut(s) 34, 206
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 45, 161, 263
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 4 cut(s) 63, 85, 117, 147
MaeI CTAG 3 cut(s) 95, 126, 227
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 10
MhlI GDGCHC 2 cut(s) 267, 287
MluCI AATT 2 cut(s) 62, 278
MnlI CCTC 2 cut(s) 26, 73
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 238
NciI CCSGG 1 cut(s) 50
NdeII GATC 1 cut(s) 33
NlaIII CATG 3 cut(s) 45, 161, 263
NsbI TGCGCA 1 cut(s) 222
NspI RCATGY 1 cut(s) 45
PciI ACATGT 1 cut(s) 41
PkrI GCNGC 3 cut(s) 215, 237, 250
PscI ACATGT 1 cut(s) 41
SatI GCNGC 3 cut(s) 214, 236, 249
Sau3AI GATC 1 cut(s) 33
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 2 cut(s) 267, 287
SetI ASST 8 cut(s) 18, 89, 111, 124, 155, 176, 276, 296
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 2 cut(s) 62, 278
SsiI CCGC 4 cut(s) 211, 214, 236, 249
SspMI CTAG 3 cut(s) 95, 126, 227
StyD4I CCNGG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 62, 278
TauI GCSGC 3 cut(s) 216, 238, 251
TscAI CASTG 1 cut(s) 60
TspRI CASTG 1 cut(s) 60
XapI RAATTY 1 cut(s) 62
XceI RCATGY 1 cut(s) 45
XspI CTAG 3 cut(s) 95, 126, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.