RLG00000005261

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004857
Physical Location & Seq
Reverse (-)
66275513 .. 66288872
13360 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005261

Sequence Viewer

Length: 702 bp
ATGGAGCTGGAGAAGAAGAAGTGCCGAAAAGAAGTGCTGACAGGCGATGTAATGGATGGGTTGATGCAAATTAAAGATGATAGAGGTAATCTGTTAAGTGCAGAAGAGGTGACAAATAACATTGTGAGCCTTGTTATTGCTGGATATGCGTCAACTTCCCTTGCATCAATGTGGGCTATCTATTTTCTTGCCAAATACCCCAATGTCCTCCAAAAGCTTCGGGAGGAGAACATGTGCGTAAGTGAGAACAAGAAAGGGGATCTTATTACAAGTGATGATATTTCAAAAATGAAATACACAAACAAGGTGGTGGAAGAGACAATAAGAATGGCAAACATTTCAGCGTTCATTTTCCGTTTGGCTACTAAGGAAGCCGAGTATAAAGGTTATAAGATACCAAAGGGTTGGAAAGTGATTCTTTGGATTCGATATATCCACACAAATTTAGAAAATTTTGGGAGTGTTTACTCATATGTCAATTGGCTGCCTCGGAAACCAGCTAAACCTGGAACGTACCAAGTTTTTGGTGCTGGACCAAGAGTTTGTGCAGGGAATATGCTGTTTAGGATACAACTTGCAATCTTATTGCATCATTTGTCTACTGGATACAAGTGGAATCTGCTGAATCCAGATGCAGGGATGGTTTATCTTCCACATCCGAAACCTATTGATAATGTTGATATTACTTTTAGAAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

234

Amino Acids

26.63

Weight (kDa)

9.33

Isoelectric Point (pI)

17.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 15 - 207 8.1e-41 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AccI GTMKAC 1 cut(s) 599
AclWI GGATC 1 cut(s) 267
AcsI RAATTY 3 cut(s) 442, 451, 696
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 1 cut(s) 635
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 1 cut(s) 285
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 3 cut(s) 7, 217, 500
AluI AGCT 3 cut(s) 7, 217, 500
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 484
ApoI RAATTY 3 cut(s) 442, 451, 696
ArsI GACNNNNNNTTYG 2 cut(s) 525, 557
AspS9I GGNCC 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 1 cut(s) 533
BbvI GCAGC 1 cut(s) 471
BccI CCATC 2 cut(s) 50, 634
BciT130I CCWGG 1 cut(s) 507
BciVI GTATCC 2 cut(s) 561, 599
BcoDI GTCTC 1 cut(s) 311
BfuI GTATCC 2 cut(s) 561, 599
BisI GCNGC 1 cut(s) 485
BlsI GCNGC 1 cut(s) 486
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 533
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 4 cut(s) 54, 173, 598, 622
BpmI CTGGAG 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 488
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 607
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 488
BseGI GGATG 3 cut(s) 61, 645, 655
BseLI CCNNNNNNNGG 1 cut(s) 635
BseNI ACTGG 1 cut(s) 607
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 471
BsgI GTGCAG 2 cut(s) 120, 567
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 311
Bsp143I GATC 1 cut(s) 259
BspPI GGATC 1 cut(s) 267
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 1 cut(s) 488
BssMI GATC 1 cut(s) 259
Bst2UI CCWGG 1 cut(s) 507
Bst6I CTCTTC 2 cut(s) 99, 309
BstDEI CTNAG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 61, 645, 655
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 2 cut(s) 405, 524
BstYI RGATCY 1 cut(s) 259
BsuI GTATCC 2 cut(s) 561, 599
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 3 cut(s) 61, 645, 655
Cfr13I GGNCC 1 cut(s) 533
CseI GACGC 1 cut(s) 138
Csp6I GTAC 1 cut(s) 514
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviAII CATG 1 cut(s) 232
CviJI RGCY 8 cut(s) 7, 129, 176, 217, 362, 374, 484, 500
CviKI_1 RGCY 8 cut(s) 7, 129, 176, 217, 362, 374, 484, 500
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
Eam1104I CTCTTC 2 cut(s) 99, 309
EarI CTCTTC 2 cut(s) 99, 309
Eco47I GGWCC 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 505
FaeI CATG 1 cut(s) 235
FaiI YATR 8 cut(s) 147, 233, 381, 390, 432, 472, 474, 557
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 402, 434
FatI CATG 1 cut(s) 231
FauNDI CATATG 1 cut(s) 472
FblI GTMKAC 1 cut(s) 599
Fnu4HI GCNGC 1 cut(s) 485
FokI GGATG 3 cut(s) 68, 642, 652
Fsp4HI GCNGC 1 cut(s) 485
GluI GCNGC 1 cut(s) 485
GsuI CTGGAG 1 cut(s) 29
HgaI GACGC 1 cut(s) 138
Hin1II CATG 1 cut(s) 235
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 215
HinfI GANTC 4 cut(s) 415, 424, 616, 625
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 3 cut(s) 153, 466, 600
Hpy188I TCNGA 2 cut(s) 492, 660
Hpy188III TCNNGA 2 cut(s) 221, 629
Hpy8I GTNNAC 3 cut(s) 153, 466, 600
HpyCH4IV ACGT 1 cut(s) 512
HpyCH4V TGCA 7 cut(s) 67, 101, 164, 548, 578, 589, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 512
Hsp92II CATG 1 cut(s) 235
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 1 cut(s) 471
LweI GCATC 4 cut(s) 54, 173, 598, 622
MaeII ACGT 1 cut(s) 512
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 5 cut(s) 25, 28, 116, 326, 641
MfeI CAATTG 1 cut(s) 478
MflI RGATCY 1 cut(s) 259
MluCI AATT 5 cut(s) 69, 442, 451, 478, 696
MmeI TCCRAC 1 cut(s) 386
MnlI CCTC 5 cut(s) 77, 100, 217, 218, 498
MseI TTAA 2 cut(s) 72, 95
MslI CAYNNNNRTG 1 cut(s) 169
MspR9I CCNGG 1 cut(s) 507
MunI CAATTG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeI CATATG 1 cut(s) 472
NdeII GATC 1 cut(s) 259
NlaIII CATG 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 235
PciI ACATGT 1 cut(s) 231
PfeI GAWTC 4 cut(s) 415, 424, 616, 625
PkrI GCNGC 1 cut(s) 486
PscI ACATGT 1 cut(s) 231
PsiI TTATAA 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PspPI GGNCC 1 cut(s) 533
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 72, 95
SatI GCNGC 1 cut(s) 485
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 1 cut(s) 533
ScrFI CCNGG 1 cut(s) 507
SfaNI GCATC 4 cut(s) 54, 173, 598, 622
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 1 cut(s) 169
Sse9I AATT 5 cut(s) 69, 442, 451, 478, 696
StyD4I CCNGG 1 cut(s) 505
TaiI ACGT 1 cut(s) 515
TaqI TCGA 1 cut(s) 427
TasI AATT 5 cut(s) 69, 442, 451, 478, 696
TfiI GAWTC 4 cut(s) 415, 424, 616, 625
Tru1I TTAA 2 cut(s) 72, 95
Tru9I TTAA 2 cut(s) 72, 95
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 1 cut(s) 484
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 2 cut(s) 305, 337
TspGWI ACGGA 1 cut(s) 344
VpaK11BI GGWCC 1 cut(s) 533
XapI RAATTY 3 cut(s) 442, 451, 696
XceI RCATGY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.