pycom03g15850

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0004857
Physical Location & Seq
Forward (+)
17103615 .. 17108358
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g15850.7

Sequence Viewer

Length: 1392 bp
ATGGAAATTTCTGAAGGAGTATGGCCGGCTGTGGTTTTGGGAGGTTTGCCACTGTTGGGGTTGCTCTTCTGGTGGTGGAATGAGCTCCGGTATGTGCTTCCTCTCAAACTACAACGTTCAGCTACTGGTGCCAAACTTCCGCCCGGTCATATGGGATTTCCATTTATTGGTGAACTGCTCACCTTCCTTTGGTACTTCAAATTTCTTTGTCGTCCAGATGAATTTATCAACGCTAAACGGGCTAAGTATGGTGATGGAGTAGGAATGTATAGAACCCACCTGTTTGGATCACCATCCATCATAACATGCTTTCCGGCGGTCAACAAATTTGTATTCCAATCATCGGATATCTTCACCCTAGTTTGGCCAAGTAATGATCTTCTGGGTCACAAATCGTTGTTTGCCGTTCATGGAAAGTCCCATGAACGACTGAAAAGCTACATCACGAAAGTAATCAATCAGCCCGACGCTCTCCGAGGCATTGCTCTCCTTGTCCAACCCCACATGATTGCTTCACTTGAATCATGGACTCAAAAGGGTAGAATTAATGCATATGATGAGGTTAAGAAGATGACTTTGGAAAATATTGGAAAGTTGTTCTTAAGTTTGGAGGCGGGACCTCTCCTAGATACCATTGATAGGTTGTTTGGTGGATTGACTGATGGACTTAGGGCTCAACCATTTAACATTCCTGGATCTGCTTTTCACCATGCTCTTCAGTGTAGGAAGAAGCTCGAGGCCATTTTCGGCAGGGAGCTAGAAAAGAAAAAAACCCAGAAAAAAGAGTTGACAAATGACCTGATGGATGGACTAATGCAAATTAAAGATGACAAAGGTGATAAATTAAGTGACCAAGAGGTGATCGATAACATTGTGAGCCTTGTTGTTGGCGGATATGCATCTACTTCCCTTGCATCAATGTGGGCTATATATTGTCTTGCCAAATACCCTAATGTCCTTGAAAAGCTTCGGGATGAGAATTTAGCTATAAGTCGGAACAAGACGGGAGACTTGATTACGAGTGAAGATGTTTCTAAAATGAAATACACAAACATGGTATTATCTGTGAATTTAAGTTATAAAATACCTAAGGGTTGGAATGTGATTCTTTGGGTTCGGTATATCCACACAGATCCAAATAACTTTGATGACCCTATGATCTTTAACCCAGATAGATGGAATGAACCAGCAAAGCCTGGAACATTCCAAGTTTTTGGTGGCGGAACAAGGATATGTGCAGGCAACATGCTTGCTAGGATGGAGCTTGCAATCTTGTTACATCATTTGTCAACAAAATACAAGTGGGAATTGCGCAATCCAAATGCCGAAGTGGTGTATCTTTCTTTTCCAAAGCCAGCTGACGGGGTTGATATCATGTTCAGCAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

464

Amino Acids

52.4

Weight (kDa)

9.0

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1080
Acc16I TGCGCA 1 cut(s) 1313
AccB1I GGYRCC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 167
AciI CCGC 5 cut(s) 140, 317, 614, 891, 1221
AclI AACGTT 1 cut(s) 115
AclWI GGATC 3 cut(s) 295, 703, 1127
AcoI YGGCCR 2 cut(s) 23, 365
AcsI RAATTY 7 cut(s) 6, 200, 221, 326, 979, 1069, 1386
AcuI CTGAAG 2 cut(s) 33, 701
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 7 cut(s) 56, 167, 189, 343, 363, 639, 1361
AflII CTTAAG 1 cut(s) 601
AgsI TTSAA 3 cut(s) 199, 521, 962
AjnI CCWGG 2 cut(s) 691, 1195
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 9 cut(s) 85, 122, 438, 733, 757, 967, 986, 1264, 1358
AluI AGCT 9 cut(s) 85, 122, 438, 733, 757, 967, 986, 1264, 1358
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 1002
AlwI GGATC 3 cut(s) 295, 703, 1127
AlwNI CAGNNNCTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 3 cut(s) 23, 365, 738
ApoI RAATTY 7 cut(s) 6, 200, 221, 326, 979, 1069, 1386
AseI ATTAAT 1 cut(s) 546
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 1 cut(s) 1314
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 8 cut(s) 172, 182, 263, 282, 346, 698, 848, 871
AvaI CYCGRG 1 cut(s) 734
AvaII GGWCC 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 1089
BalI TGGCCA 1 cut(s) 367
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 2 cut(s) 87, 676
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 8 cut(s) 248, 301, 305, 656, 796, 800, 1170, 1252
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 2 cut(s) 693, 1197
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 1002
BfaI CTAG 4 cut(s) 359, 626, 758, 1254
BfrI CTTAAG 1 cut(s) 601
Bme1390I CCNGG 3 cut(s) 144, 693, 1197
Bme18I GGWCC 1 cut(s) 617
BmeT110I CYCGRG 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 130, 618
BmrFI CCNGG 3 cut(s) 144, 693, 1197
BmsI GCATC 2 cut(s) 908, 923
BpuMI CCSGG 1 cut(s) 144
Bsa29I ATCGAT 1 cut(s) 864
BsaBI GATNNNNATC 2 cut(s) 292, 898
BsaJI CCNNGG 1 cut(s) 475
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 7 cut(s) 56, 167, 189, 343, 363, 639, 1361
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 130
Bse21I CCTNAGG 1 cut(s) 1089
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 2 cut(s) 292, 898
BseBI CCWGG 2 cut(s) 693, 1197
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 4 cut(s) 293, 811, 979, 1263
BseJI GATNNNNATC 2 cut(s) 292, 898
BseLI CCNNNNNNNGG 7 cut(s) 56, 167, 189, 343, 363, 639, 1361
BseMI GCAATG 1 cut(s) 480
BseNI ACTGG 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 1257
BshFI GGCC 3 cut(s) 25, 367, 740
BshNI GGYRCC 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 864
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 4 cut(s) 26, 88, 144, 314
BslFI GGGAC 2 cut(s) 403, 630
BslI CCNNNNNNNGG 7 cut(s) 56, 167, 189, 343, 363, 639, 1361
BsmAI GTCTC 1 cut(s) 1002
BsmFI GGGAC 2 cut(s) 403, 630
BsnI GGCC 3 cut(s) 25, 367, 740
BsoBI CYCGRG 1 cut(s) 734
Bsp1286I GDGCHC 2 cut(s) 87, 676
Bsp143I GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
BspACI CCGC 5 cut(s) 140, 317, 614, 891, 1221
BspANI GGCC 3 cut(s) 25, 367, 740
BspDI ATCGAT 1 cut(s) 864
BspLI GGNNCC 2 cut(s) 130, 618
BspPI GGATC 3 cut(s) 295, 703, 1127
BspQI GCTCTTC 2 cut(s) 71, 720
BspT107I GGYRCC 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 601
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
Bst2UI CCWGG 2 cut(s) 693, 1197
Bst4CI ACNGT 1 cut(s) 54
Bst6I CTCTTC 2 cut(s) 71, 720
BstAFI CTTAAG 1 cut(s) 601
BstC8I GCNNGC 5 cut(s) 27, 1240, 1251, 1266, 1356
BstDEI CTNAG 3 cut(s) 243, 668, 1089
BstF5I GGATG 4 cut(s) 293, 811, 979, 1263
BstHHI GCGC 1 cut(s) 1314
BstKTI GATC 6 cut(s) 290, 379, 698, 864, 1135, 1161
BstMAI GTCTC 1 cut(s) 1002
BstMBI GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
BstMWI GCNNNNNNNGC 2 cut(s) 128, 239
BstNI CCWGG 2 cut(s) 693, 1197
BstNSI RCATGY 2 cut(s) 309, 1249
BstSCI CCNGG 3 cut(s) 142, 691, 1195
BstX2I RGATCY 2 cut(s) 695, 1132
BstXI CCANNNNNNTGG 3 cut(s) 284, 1176, 1214
BstYI RGATCY 2 cut(s) 695, 1132
Bsu15I ATCGAT 1 cut(s) 864
Bsu36I CCTNAGG 1 cut(s) 1089
BsuRI GGCC 3 cut(s) 25, 367, 740
BsuTUI ATCGAT 1 cut(s) 864
BtsCI GGATG 4 cut(s) 293, 811, 979, 1263
BtsIMutI CAGTG 2 cut(s) 50, 725
Cac8I GCNNGC 5 cut(s) 27, 1240, 1251, 1266, 1356
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 1314
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 617
ClaI ATCGAT 1 cut(s) 864
CseI GACGC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 193
CviAII CATG 9 cut(s) 306, 410, 422, 505, 525, 710, 1054, 1246, 1375
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 3 cut(s) 243, 668, 1089
DpnI GATC 6 cut(s) 289, 378, 697, 863, 1134, 1160
DpnII GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
EaeI YGGCCR 2 cut(s) 23, 365
Eam1104I CTCTTC 2 cut(s) 71, 720
EarI CTCTTC 2 cut(s) 71, 720
EciI GGCGGA 3 cut(s) 129, 906, 1236
Ecl136II GAGCTC 1 cut(s) 85
Eco24I GRGCYC 2 cut(s) 87, 676
Eco32I GATATC 2 cut(s) 349, 1372
Eco47I GGWCC 1 cut(s) 617
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 2 cut(s) 33, 701
Eco81I CCTNAGG 1 cut(s) 1089
Eco88I CYCGRG 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 617
EcoRII CCWGG 2 cut(s) 691, 1195
EcoRV GATATC 2 cut(s) 349, 1372
EcoT22I ATGCAT 2 cut(s) 553, 901
EcoT38I GRGCYC 2 cut(s) 87, 676
FaeI CATG 9 cut(s) 309, 413, 425, 508, 528, 713, 1057, 1249, 1378
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 2 cut(s) 403, 630
FatI CATG 9 cut(s) 305, 409, 421, 504, 524, 709, 1053, 1245, 1374
FauI CCCGC 1 cut(s) 607
FauNDI CATATG 2 cut(s) 150, 553
FokI GGATG 4 cut(s) 280, 818, 986, 1270
FriOI GRGCYC 2 cut(s) 87, 676
FspBI CTAG 4 cut(s) 359, 626, 758, 1254
FspI TGCGCA 1 cut(s) 1313
GlaI GCGC 1 cut(s) 1313
HaeIII GGCC 3 cut(s) 25, 367, 740
HapII CCGG 4 cut(s) 26, 88, 144, 314
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 1314
Hin1II CATG 9 cut(s) 309, 413, 425, 508, 528, 713, 1057, 1249, 1378
Hin6I GCGC 1 cut(s) 1312
HinP1I GCGC 1 cut(s) 1312
HincII GTYRAC 3 cut(s) 322, 789, 1290
HindII GTYRAC 3 cut(s) 322, 789, 1290
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 3 cut(s) 521, 529, 1105
HpaII CCGG 4 cut(s) 26, 88, 144, 314
HphI GGTGA 8 cut(s) 172, 182, 263, 282, 346, 698, 848, 871
Hpy166II GTNNAC 4 cut(s) 173, 322, 789, 1290
Hpy188I TCNGA 4 cut(s) 13, 346, 476, 996
Hpy188III TCNNGA 3 cut(s) 215, 445, 971
Hpy8I GTNNAC 4 cut(s) 173, 322, 789, 1290
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 2 cut(s) 8, 193
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 6 cut(s) 551, 817, 899, 914, 1238, 1268
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 239
HpyF3I CTNAG 3 cut(s) 243, 668, 1089
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 9 cut(s) 309, 413, 425, 508, 528, 713, 1057, 1249, 1378
HspAI GCGC 1 cut(s) 1312
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Kzo9I GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
LguI GCTCTTC 2 cut(s) 71, 720
LmnI GCTCC 3 cut(s) 90, 754, 1261
LweI GCATC 2 cut(s) 908, 923
MaeI CTAG 4 cut(s) 359, 626, 758, 1254
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 3 cut(s) 386, 848, 1275
MalI GATC 6 cut(s) 289, 378, 697, 863, 1134, 1160
MboI GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
MboII GAAGA 7 cut(s) 58, 343, 371, 580, 707, 739, 1037
MflI RGATCY 2 cut(s) 695, 1132
MhlI GDGCHC 2 cut(s) 87, 676
MlsI TGGCCA 1 cut(s) 367
MluNI TGGCCA 1 cut(s) 367
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 3 cut(s) 520, 974, 1076
MnlI CCTC 8 cut(s) 35, 111, 470, 553, 604, 630, 730, 850
Mox20I TGGCCA 1 cut(s) 367
Mph1103I ATGCAT 2 cut(s) 553, 901
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 1 cut(s) 966
MscI TGGCCA 1 cut(s) 367
MseI TTAA 9 cut(s) 546, 564, 602, 684, 822, 845, 1073, 1164, 1390
MslI CAYNNNNRTG 2 cut(s) 919, 1052
Msp20I TGGCCA 1 cut(s) 367
MspA1I CMGCKG 1 cut(s) 1358
MspCI CTTAAG 1 cut(s) 601
MspI CCGG 4 cut(s) 26, 88, 144, 314
MspR9I CCNGG 3 cut(s) 144, 693, 1197
MvaI CCWGG 2 cut(s) 693, 1197
MwoI GCNNNNNNNGC 2 cut(s) 128, 239
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 144
NdeI CATATG 2 cut(s) 150, 553
NdeII GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 9 cut(s) 309, 413, 425, 508, 528, 713, 1057, 1249, 1378
NlaIV GGNNCC 2 cut(s) 130, 618
NmuCI GTSAC 2 cut(s) 386, 848
NsbI TGCGCA 1 cut(s) 1313
NsiI ATGCAT 2 cut(s) 553, 901
NspI RCATGY 2 cut(s) 309, 1249
PaeR7I CTCGAG 1 cut(s) 734
PciSI GCTCTTC 2 cut(s) 71, 720
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 2 cut(s) 521, 1105
PflMI CCANNNNNTGG 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 691
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
PpuMI RGGWCCY 1 cut(s) 617
PshBI ATTAAT 1 cut(s) 546
PsiI TTATAA 1 cut(s) 1080
Psp124BI GAGCTC 1 cut(s) 87
Psp1406I AACGTT 1 cut(s) 115
Psp5II RGGWCCY 1 cut(s) 617
Psp6I CCWGG 2 cut(s) 691, 1195
PspGI CCWGG 2 cut(s) 691, 1195
PspN4I GGNNCC 2 cut(s) 130, 618
PspPI GGNCC 1 cut(s) 617
PspPPI RGGWCCY 1 cut(s) 617
PspXI VCTCGAGB 1 cut(s) 734
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 2 cut(s) 695, 1132
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 2 cut(s) 919, 1052
SacI GAGCTC 1 cut(s) 87
SapI GCTCTTC 2 cut(s) 71, 720
SaqAI TTAA 9 cut(s) 546, 564, 602, 684, 822, 845, 1073, 1164, 1390
Sau3AI GATC 6 cut(s) 287, 376, 695, 861, 1132, 1158
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 3 cut(s) 144, 693, 1197
SduI GDGCHC 2 cut(s) 87, 676
SfaNI GCATC 2 cut(s) 908, 923
Sfr274I CTCGAG 1 cut(s) 734
SinI GGWCC 1 cut(s) 617
SlaI CTCGAG 1 cut(s) 734
SmiMI CAYNNNNRTG 2 cut(s) 919, 1052
SmlI CTYRAG 2 cut(s) 601, 734
SmoI CTYRAG 2 cut(s) 601, 734
SsiI CCGC 5 cut(s) 140, 317, 614, 891, 1221
SspI AATATT 1 cut(s) 586
SspMI CTAG 4 cut(s) 359, 626, 758, 1254
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 3 cut(s) 142, 691, 1195
TaaI ACNGT 1 cut(s) 54
TaiI ACGT 1 cut(s) 118
TaqI TCGA 2 cut(s) 735, 864
TfiI GAWTC 2 cut(s) 521, 1105
Tru1I TTAA 9 cut(s) 546, 564, 602, 684, 822, 845, 1073, 1164, 1390
Tru9I TTAA 9 cut(s) 546, 564, 602, 684, 822, 845, 1073, 1164, 1390
TscAI CASTG 2 cut(s) 57, 725
TseFI GTSAC 2 cut(s) 386, 848
Tsp45I GTSAC 2 cut(s) 386, 848
TspDTI ATGAA 5 cut(s) 234, 398, 438, 1055, 1197
TspRI CASTG 2 cut(s) 57, 725
Van91I CCANNNNNTGG 1 cut(s) 167
Vha464I CTTAAG 1 cut(s) 601
VpaK11BI GGWCC 1 cut(s) 617
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 7 cut(s) 6, 200, 221, 326, 979, 1069, 1386
XceI RCATGY 2 cut(s) 309, 1249
XcmI CCANNNNNNNNNTGG 1 cut(s) 1214
XhoI CTCGAG 1 cut(s) 734
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 4 cut(s) 359, 626, 758, 1254
Zsp2I ATGCAT 2 cut(s) 553, 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.