MD14G1193600.v1.1

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004857
Physical Location & Seq
Forward (+)
28482340 .. 28486023
3684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1193600.v1.1.491

Sequence Viewer

Length: 222 bp
ATGAAATACACAAACAAGGTGGTGGAAGAAACAATAAGATTGGCCAACGTTGCAGCATTTGTTTTCCGATTGGCTACTAAGGAAGTTGAGTATAAAGGTTATAAAATACCCAAGGATTGGAGAGTGATTGTTTGGGTTCGATATATCCACACAAATTCAGAGAATTTTGATGATCCCATGTGCTTCAACCCAGATAGATGGAATGAATCAGCTAAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

74

Amino Acids

8.79

Weight (kDa)

8.79

Isoelectric Point (pI)

14.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 70 1.3e-18 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AccB7I CCANNNNNTGG 1 cut(s) 117
AclI AACGTT 1 cut(s) 48
AclWI GGATC 1 cut(s) 167
AcoI YGGCCR 1 cut(s) 42
AcsI RAATTY 2 cut(s) 154, 163
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 1 cut(s) 187
AluBI AGCT 2 cut(s) 212, 217
AluI AGCT 2 cut(s) 212, 217
AlwI GGATC 1 cut(s) 167
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 53
ApoI RAATTY 2 cut(s) 154, 163
BalI TGGCCA 1 cut(s) 44
BbvI GCAGC 1 cut(s) 65
BccI CCATC 1 cut(s) 192
BisI GCNGC 1 cut(s) 54
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 117
BseXI GCAGC 1 cut(s) 65
BshFI GGCC 1 cut(s) 44
BslI CCNNNNNNNGG 1 cut(s) 117
BsnI GGCC 1 cut(s) 44
Bsp143I GATC 1 cut(s) 172
Bsp1720I GCTNAGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 44
BspPI GGATC 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 1 cut(s) 172
BssT1I CCWWGG 1 cut(s) 111
BstDEI CTNAG 2 cut(s) 78, 213
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 198
BsuRI GGCC 1 cut(s) 44
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 1 cut(s) 178
CviJI RGCY 4 cut(s) 44, 74, 212, 217
CviKI_1 RGCY 4 cut(s) 44, 74, 212, 217
DdeI CTNAG 2 cut(s) 78, 213
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 1 cut(s) 181
FaiI YATR 4 cut(s) 93, 102, 144, 179
FatI CATG 1 cut(s) 177
Fnu4HI GCNGC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 44
Hin1II CATG 1 cut(s) 181
HinfI GANTC 1 cut(s) 206
Hpy188I TCNGA 3 cut(s) 68, 160, 221
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 2 cut(s) 78, 213
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 1 cut(s) 181
Kzo9I GATC 1 cut(s) 172
LpnPI CCDG 1 cut(s) 204
Lsp1109I GCAGC 1 cut(s) 65
MaeII ACGT 1 cut(s) 48
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 1 cut(s) 38
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 154, 163
MluNI TGGCCA 1 cut(s) 44
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 44
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 1 cut(s) 172
NlaIII CATG 1 cut(s) 181
PfeI GAWTC 1 cut(s) 206
PflMI CCANNNNNTGG 1 cut(s) 117
PkrI GCNGC 1 cut(s) 55
PsiI TTATAA 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 48
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 1 cut(s) 172
SetI ASST 5 cut(s) 21, 51, 100, 214, 219
SgeI CNNG 4 cut(s) 28, 124, 190, 203
Sse9I AATT 2 cut(s) 154, 163
StyI CCWWGG 1 cut(s) 111
TaiI ACGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 139
TasI AATT 2 cut(s) 154, 163
TfiI GAWTC 1 cut(s) 206
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 2 cut(s) 17, 219
Van91I CCANNNNNTGG 1 cut(s) 117
XapI RAATTY 2 cut(s) 154, 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.