FvH4_5g15590

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004857
Physical Location & Seq
Reverse (-)
8798029 .. 8800487
2459 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15590.t1

Sequence Viewer

Length: 1368 bp
ATGGAAATCCCTATGGGAATGGGGGTAGCCTTCTTAGTCGGTGGTTTGCCAATATTAGGGCTGCTCTTGTGGTGGTGGAATGAGCTTTGGTACGTGCTTCCTCTCAAATTGAGACTTCCGGCCACCAAAGGCAACTTGCCCCCAGGTCATATGGGATTTCCTTTTTTCGGCGAAATGTTCAATTTTCTTTGGTATTTCAAAATTCTTCGACATCCAGATGAATTTATCAATATTAAACGAGCAAAATACGGTGATGGAGTAGGAATGTATAGAACTCATCTATTCGGATCACCAACCATTATAACATGCTTTCCAGATGTGAATAAGTATGTGTTCCAATCGGAGGAGACATTCTTTCTCGATTGGCCCAGAGTTGAACTCATGGGTCAAAACTCTATCGTTGCGGTTCATGGACCGGCCCATGTAAGACTTAGAAGATATGTTATTGGTGTGATTAATCGGCCCGACTCTCTCCGACACATTGCTCAAGTAGTCCAACCCCGGATGATTGCAGCACTCAAGTCATGGGCCCAAAAGGGTAGAATCAAAGCATACGATGAAGCTAAGAAGGTGACATTTGAAAATATTGGGAAACTATTTGCAAGTTTGGAGCCCGGACCTCTCCTAGATACAATTGATGACCTGTTTACAGGATTGATCAAAGGAATTAGGGCTCAACCGTTCAATATTCCGGGATCTGCTTGTCACCATGCTCGTCAGTGTAGGAAGAAGCTTGACGCCATTTTCTGGATGGAGCTAGAGAAGAAAAAGAAGAACCAAAAAGAAGTTAGCACAAATGATCTTATGGATGGGCTTATGCAGATTAAAGATGATGAGGGAAATAAACTAGGTGACCAAGAAGTGATAGATAACATTGTCAGTCTTGTTGTTGCTGGATATGCATCTACTTCCCTTGCATCAATGTGGGCGATATATTATCTTGCCAAATTCCCAAATGTCCTCCAAAAGCTTCGGGAGGAGAATATGGCTATACGTAAGACCAAGGAAGGAGATTTCATTACAAGTGAAGATGTCTCCAAAATGAAATACACAAACAAGGTGGTGGAAGAAACAATAAGAATGGCTAACATTGCAGCATTCATTTTCCGGACTGCTACCAAGGATGCTGAGTACAAAGGTTATATTATACCGAAGGGTTGGCAAGTGATTGTTTGGGTTCGGTATCTCCATACAAATTCGGAATATTTTGATGATCCTATGTGTTTCAACCCAAATCGATGGAATGATCCAGCTAGACCAGGAACTTATCAAGTTTTTGGCGGTGGATCAAGAATATGTGCAGGCAATATGCTTGTTAGGATTCAACTCGCAGTGTTTTTGCACCATCTATCTACAGCATACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

456

Amino Acids

52.12

Weight (kDa)

9.15

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 450 2.7e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 302
AccB7I CCANNNNNTGG 1 cut(s) 747
AccIII TCCGGA 1 cut(s) 1107
AciI CCGC 2 cut(s) 404, 1281
AclWI GGATC 5 cut(s) 295, 703, 1208, 1241, 1294
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 4 cut(s) 201, 221, 947, 1195
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 2 cut(s) 92, 1133
AfiI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 747
AgsI TTSAA 7 cut(s) 181, 199, 377, 581, 685, 1228, 1325
AjnI CCWGG 2 cut(s) 142, 1258
AluBI AGCT 6 cut(s) 85, 563, 733, 757, 970, 1253
AluI AGCT 6 cut(s) 85, 563, 733, 757, 970, 1253
Alw26I GTCTC 3 cut(s) 106, 341, 1039
AlwI GGATC 5 cut(s) 295, 703, 1208, 1241, 1294
Aor13HI TCCGGA 1 cut(s) 1107
AoxI GGCC 5 cut(s) 120, 365, 417, 461, 528
ApaI GGGCCC 1 cut(s) 532
ApeKI GCWGC 3 cut(s) 61, 512, 1094
ApoI RAATTY 4 cut(s) 201, 221, 947, 1195
AseI ATTAAT 1 cut(s) 456
AspS9I GGNCC 7 cut(s) 366, 413, 418, 462, 528, 529, 617
AsuC2I CCSGG 3 cut(s) 502, 615, 693
AsuHPI GGTGA 5 cut(s) 263, 282, 583, 698, 863
AvaII GGWCC 2 cut(s) 413, 617
BaeGI GKGCMC 1 cut(s) 532
BanII GRGCYC 3 cut(s) 532, 615, 676
BbvI GCAGC 3 cut(s) 48, 524, 1106
BccI CCATC 5 cut(s) 248, 745, 803, 1233, 1353
BciT130I CCWGG 2 cut(s) 144, 1260
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 3 cut(s) 502, 615, 693
BcoDI GTCTC 3 cut(s) 106, 341, 1039
BfaI CTAG 4 cut(s) 626, 758, 848, 1254
BfmI CTRYAG 1 cut(s) 1353
BisI GCNGC 3 cut(s) 62, 513, 1095
BlsI GCNGC 3 cut(s) 63, 514, 1096
Bme1390I CCNGG 5 cut(s) 144, 502, 615, 693, 1260
Bme18I GGWCC 2 cut(s) 413, 617
BmgT120I GGNCC 7 cut(s) 366, 413, 418, 462, 528, 529, 617
BmiI GGNNCC 2 cut(s) 530, 612
BmrFI CCNGG 5 cut(s) 144, 502, 615, 693, 1260
BmsI GCATC 3 cut(s) 911, 926, 1114
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BpuEI CTTGAG 2 cut(s) 471, 503
BpuMI CCSGG 3 cut(s) 502, 615, 693
Bsa29I ATCGAT 1 cut(s) 1237
BsaAI YACGTR 2 cut(s) 94, 995
BsaBI GATNNNNATC 1 cut(s) 901
BsaHI GRCGYC 1 cut(s) 738
BsaJI CCNNGG 4 cut(s) 142, 500, 1002, 1119
BsaWI WCCGGW 1 cut(s) 1107
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 167, 343, 747
Bse118I RCCGGY 1 cut(s) 415
Bse3DI GCAATG 2 cut(s) 480, 1089
Bse8I GATNNNNATC 1 cut(s) 901
BseAI TCCGGA 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 144, 1260
BseCI ATCGAT 1 cut(s) 1237
BseDI CCNNGG 4 cut(s) 142, 500, 1002, 1119
BseGI GGATG 5 cut(s) 211, 510, 756, 814, 1129
BseJI GATNNNNATC 1 cut(s) 901
BseLI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 747
BseMI GCAATG 2 cut(s) 480, 1089
BseMII CTCAG 1 cut(s) 1119
BseRI GAGGAG 2 cut(s) 359, 992
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 3 cut(s) 48, 524, 1106
BsgI GTGCAG 1 cut(s) 1320
BshFI GGCC 5 cut(s) 122, 367, 419, 463, 530
BshVI ATCGAT 1 cut(s) 1237
BsiSI CCGG 6 cut(s) 119, 416, 502, 615, 692, 1108
BslI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 747
BsmAI GTCTC 3 cut(s) 106, 341, 1039
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 5 cut(s) 122, 367, 419, 463, 530
Bsp120I GGGCCC 1 cut(s) 528
Bsp1286I GDGCHC 3 cut(s) 532, 615, 676
Bsp13I TCCGGA 1 cut(s) 1107
Bsp143I GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
BspACI CCGC 2 cut(s) 404, 1281
BspANI GGCC 5 cut(s) 122, 367, 419, 463, 530
BspCNI CTCAG 1 cut(s) 1120
BspDI ATCGAT 1 cut(s) 1237
BspEI TCCGGA 1 cut(s) 1107
BspLI GGNNCC 2 cut(s) 530, 612
BspPI GGATC 5 cut(s) 295, 703, 1208, 1241, 1294
BsrDI GCAATG 2 cut(s) 480, 1089
BsrFI RCCGGY 1 cut(s) 415
BssAI RCCGGY 1 cut(s) 415
BssECI CCNNGG 4 cut(s) 142, 500, 1002, 1119
BssMI GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
BssNI GRCGYC 1 cut(s) 738
BssT1I CCWWGG 2 cut(s) 1002, 1119
Bst2UI CCWGG 2 cut(s) 144, 1260
Bst4CI ACNGT 2 cut(s) 251, 681
BstACI GRCGYC 1 cut(s) 738
BstBAI YACGTR 2 cut(s) 94, 995
BstC8I GCNNGC 1 cut(s) 1303
BstDEI CTNAG 4 cut(s) 34, 431, 564, 1128
BstEII GGTNACC 1 cut(s) 851
BstF5I GGATG 5 cut(s) 211, 510, 756, 814, 1129
BstKTI GATC 7 cut(s) 290, 660, 698, 802, 1216, 1249, 1289
BstMAI GTCTC 3 cut(s) 106, 341, 1039
BstMBI GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
BstMWI GCNNNNNNNGC 2 cut(s) 899, 1091
BstNI CCWGG 2 cut(s) 144, 1260
BstNSI RCATGY 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 5 cut(s) 142, 500, 613, 691, 1258
BstSFI CTRYAG 1 cut(s) 1353
BstSLI GKGCMC 1 cut(s) 532
BstSNI TACGTA 1 cut(s) 995
BstV1I GCAGC 3 cut(s) 48, 524, 1106
BstX2I RGATCY 1 cut(s) 695
BstXI CCANNNNNNTGG 1 cut(s) 1239
BstYI RGATCY 1 cut(s) 695
Bsu15I ATCGAT 1 cut(s) 1237
BsuRI GGCC 5 cut(s) 122, 367, 419, 463, 530
BsuTUI ATCGAT 1 cut(s) 1237
BtsCI GGATG 5 cut(s) 211, 510, 756, 814, 1129
BtsI GCAGTG 1 cut(s) 1338
BtsIMutI CAGTG 2 cut(s) 725, 1338
Cac8I GCNNGC 1 cut(s) 1303
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 7 cut(s) 366, 413, 418, 462, 528, 529, 617
ClaI ATCGAT 1 cut(s) 1237
CseI GACGC 1 cut(s) 746
Csp6I GTAC 2 cut(s) 91, 1132
CspCI CAANNNNNGTGG 2 cut(s) 1041, 1076
CviAII CATG 6 cut(s) 306, 382, 410, 422, 525, 710
CviQI GTAC 2 cut(s) 91, 1132
DdeI CTNAG 4 cut(s) 34, 431, 564, 1128
DpnI GATC 7 cut(s) 289, 659, 697, 801, 1215, 1248, 1288
DpnII GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
EaeI YGGCCR 1 cut(s) 120
Eco105I TACGTA 1 cut(s) 995
Eco130I CCWWGG 2 cut(s) 1002, 1119
Eco24I GRGCYC 3 cut(s) 532, 615, 676
Eco47I GGWCC 2 cut(s) 413, 617
Eco91I GGTNACC 1 cut(s) 851
EcoO65I GGTNACC 1 cut(s) 851
EcoRII CCWGG 2 cut(s) 142, 1258
EcoT14I CCWWGG 2 cut(s) 1002, 1119
EcoT22I ATGCAT 1 cut(s) 904
EcoT38I GRGCYC 3 cut(s) 532, 615, 676
ErhI CCWWGG 2 cut(s) 1002, 1119
FaeI CATG 6 cut(s) 309, 385, 413, 425, 528, 713
FatI CATG 6 cut(s) 305, 381, 409, 421, 524, 709
FauNDI CATATG 1 cut(s) 150
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 62, 513, 1095
FokI GGATG 5 cut(s) 198, 517, 763, 821, 1136
FriOI GRGCYC 3 cut(s) 532, 615, 676
Fsp4HI GCNGC 3 cut(s) 62, 513, 1095
FspBI CTAG 4 cut(s) 626, 758, 848, 1254
GluI GCNGC 3 cut(s) 62, 513, 1095
HaeIII GGCC 5 cut(s) 122, 367, 419, 463, 530
HapII CCGG 6 cut(s) 119, 416, 502, 615, 692, 1108
HgaI GACGC 1 cut(s) 746
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 6 cut(s) 309, 385, 413, 425, 528, 713
HindIII AAGCTT 2 cut(s) 731, 968
HinfI GANTC 3 cut(s) 467, 543, 1321
HpaII CCGG 6 cut(s) 119, 416, 502, 615, 692, 1108
HphI GGTGA 5 cut(s) 263, 282, 583, 698, 863
Hpy166II GTNNAC 1 cut(s) 648
Hpy188I TCNGA 4 cut(s) 287, 343, 476, 1201
Hpy188III TCNNGA 7 cut(s) 215, 314, 359, 748, 974, 1108, 1290
Hpy8I GTNNAC 1 cut(s) 648
HpyAV CCTTC 4 cut(s) 40, 562, 1001, 1147
HpyCH4III ACNGT 2 cut(s) 251, 681
HpyCH4IV ACGT 2 cut(s) 93, 994
HpyCH4V TGCA 8 cut(s) 512, 602, 820, 902, 917, 1094, 1301, 1342
HpyF10VI GCNNNNNNNGC 2 cut(s) 899, 1091
HpyF3I CTNAG 4 cut(s) 34, 431, 564, 1128
HpySE526I ACGT 2 cut(s) 93, 994
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 6 cut(s) 309, 385, 413, 425, 528, 713
Kpn2I TCCGGA 1 cut(s) 1107
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
LmnI GCTCC 2 cut(s) 610, 754
Lsp1109I GCAGC 3 cut(s) 48, 524, 1106
LweI GCATC 3 cut(s) 911, 926, 1114
MaeI CTAG 4 cut(s) 626, 758, 848, 1254
MaeII ACGT 2 cut(s) 93, 994
MaeIII GTNAC 3 cut(s) 571, 704, 851
MalI GATC 7 cut(s) 289, 659, 697, 801, 1215, 1248, 1288
MboI GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
MboII GAAGA 7 cut(s) 197, 447, 739, 775, 784, 1040, 1079
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 3 cut(s) 532, 615, 676
MluCI AATT 8 cut(s) 107, 181, 201, 221, 633, 666, 947, 1195
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 499, 520
MnlI CCTC 6 cut(s) 111, 337, 630, 829, 970, 971
Mph1103I ATGCAT 1 cut(s) 904
MroI TCCGGA 1 cut(s) 1107
MseI TTAA 3 cut(s) 234, 456, 825
MslI CAYNNNNRTG 3 cut(s) 216, 922, 1363
MspI CCGG 6 cut(s) 119, 416, 502, 615, 692, 1108
MspR9I CCNGG 5 cut(s) 144, 502, 615, 693, 1260
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 2 cut(s) 144, 1260
MwoI GCNNNNNNNGC 2 cut(s) 899, 1091
NciI CCSGG 3 cut(s) 502, 615, 693
NdeI CATATG 1 cut(s) 150
NdeII GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
NlaIII CATG 6 cut(s) 309, 385, 413, 425, 528, 713
NlaIV GGNNCC 2 cut(s) 530, 612
NmuCI GTSAC 3 cut(s) 571, 704, 851
NsiI ATGCAT 1 cut(s) 904
NspI RCATGY 1 cut(s) 309
PctI GAATGC 1 cut(s) 1097
PfeI GAWTC 2 cut(s) 543, 1321
PflMI CCANNNNNTGG 1 cut(s) 747
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 3 cut(s) 63, 514, 1096
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Ppu21I YACGTR 2 cut(s) 94, 995
PshBI ATTAAT 1 cut(s) 456
PsiI TTATAA 1 cut(s) 302
Psp6I CCWGG 2 cut(s) 142, 1258
PspEI GGTNACC 1 cut(s) 851
PspGI CCWGG 2 cut(s) 142, 1258
PspN4I GGNNCC 2 cut(s) 530, 612
PspOMI GGGCCC 1 cut(s) 528
PspPI GGNCC 7 cut(s) 366, 413, 418, 462, 528, 529, 617
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 2 cut(s) 92, 1133
RsaNI GTAC 2 cut(s) 91, 1132
RseI CAYNNNNRTG 3 cut(s) 216, 922, 1363
SaqAI TTAA 3 cut(s) 234, 456, 825
SatI GCNGC 3 cut(s) 62, 513, 1095
Sau3AI GATC 7 cut(s) 287, 657, 695, 799, 1213, 1246, 1286
Sau96I GGNCC 7 cut(s) 366, 413, 418, 462, 528, 529, 617
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 5 cut(s) 144, 502, 615, 693, 1260
SduI GDGCHC 3 cut(s) 532, 615, 676
SfaNI GCATC 3 cut(s) 911, 926, 1114
SfcI CTRYAG 1 cut(s) 1353
SinI GGWCC 2 cut(s) 413, 617
SmiMI CAYNNNNRTG 3 cut(s) 216, 922, 1363
SmlI CTYRAG 2 cut(s) 486, 518
SmoI CTYRAG 2 cut(s) 486, 518
SnaBI TACGTA 1 cut(s) 995
Sse9I AATT 8 cut(s) 107, 181, 201, 221, 633, 666, 947, 1195
SsiI CCGC 2 cut(s) 404, 1281
SspI AATATT 5 cut(s) 54, 232, 586, 688, 1205
SspMI CTAG 4 cut(s) 626, 758, 848, 1254
StyD4I CCNGG 5 cut(s) 142, 500, 613, 691, 1258
StyI CCWWGG 2 cut(s) 1002, 1119
TaaI ACNGT 2 cut(s) 251, 681
TaiI ACGT 2 cut(s) 96, 997
TaqI TCGA 3 cut(s) 208, 360, 1237
TasI AATT 8 cut(s) 107, 181, 201, 221, 633, 666, 947, 1195
TatI WGTACW 1 cut(s) 1131
TfiI GAWTC 2 cut(s) 543, 1321
Tru1I TTAA 3 cut(s) 234, 456, 825
Tru9I TTAA 3 cut(s) 234, 456, 825
TscAI CASTG 2 cut(s) 725, 1338
TseFI GTSAC 3 cut(s) 571, 704, 851
TseI GCWGC 3 cut(s) 61, 512, 1094
Tsp45I GTSAC 3 cut(s) 571, 704, 851
TspDTI ATGAA 6 cut(s) 234, 398, 573, 1006, 1058, 1090
TspRI CASTG 2 cut(s) 725, 1338
Van91I CCANNNNNTGG 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 413, 617
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 4 cut(s) 201, 221, 947, 1195
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
XspI CTAG 4 cut(s) 626, 758, 848, 1254
Zsp2I ATGCAT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.