MD14G1193400.v1.1

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004857
Physical Location & Seq
Forward (+)
28438791 .. 28441524
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1193400.v1.1.491

Sequence Viewer

Length: 1452 bp
ATGAGTATTTCTATGGGAGAGTGGGTGACCTTGGTTTTGGGAAGTTTTCCATTGTTGGGGTTGCTATTGTGGTGGTGGAATGAGCTTTGGTATGTGCTTCCTCTCAAACTACGACATTCGCCTACCGGCGCTAAATTGCCGCCCGGCCACATGGGACTTCCATTCTTGGGTGAAATCTTCACCTTCCATTGGTACTTCAAAATTGTTCGTCGTCCTGACGACTTTATCAATGCTAAGCGGGCCAAGTACGGCGATGGAGTCGGAATGTATAGAACTCACCTCTTTGGATCACCGACCATCATAGCATGCTTCCCGGGAATCAACAAATTTGTGTTCCAATCAGATGAAACCTTCATCCTAGACTGGCCCAATGTTGATATTGTAGGTGCTAGTTCTATAGTGGCAGTTCATGGAAAGTCTCATACAAAAGTTAGAAGCTATGTTGTAAGCGTAATTAACCGTCCTGACGCTCTCCGTCGCATAAGCCAGCTTGTCCAACCCCGTATGGTGGCCGCGTTGGAGTCATGGGCTCTGAAGGGTAGAGTCAAAGGATATGATGAAGCCAGGAAGGTCACATTTGAAAATATTGGGAAACTATTCGTAAGTTTGGAGCCGGGGCCTCTCCTTGATACCATTGATAACTTGTTTACTGGATTAGTCACTGGAATTAGGGCTCATCCACTGAACATTCCCGGATCGGCTTACCGCCATGCCCTTCAGTGTAGGAAAAAGCTTGAGGACATTTTCAGGGTGGAGCTAGAGAAGAAAAAAACTCTAAAGGAAGGGGTGACAAATGATCTAATGGATGGGCTTATGCAGATTAAAGACGATGAAGGCAATAAATTAAGTGACCAAGAGGTTCTAGATAACATTGTCAGCCTTGTTGTCGCTGGATATGCATCTACTTCGCTATCAACAATGTGGACTTTATATTATCTCGCAAAATACCCTGACGTCCTCCAAAAGCTTTGCGAAGAGAATATGGCTATACATGAGAACAAGACTGGGGAGTTTATCACGTATGAAGATGTTTTAAAAATGAAATATACAAACAGGGTGGTGGAAGAAACAATAAGATTGGCCAACATTGCAGCATTTGTTTTCCGATTGGCTACTAAGGAAGTTGAGTATAAAGGTTATAAAATACCCAAGGATTGGAGAGTGATTGTTTGGGTTCGATATCTCCACACAAATTCAGAGAATTTTGATGATCCCATGTGCTTCAACCCAGATAGATGGAATGAACCAGCTAAGCCAGGAACATACCAAGTTTTTGGTGGTGGATCGAGAATATGTGCAGGAAACTTGCTTGTCAAGATACAACTTGCAGTATTTTTACATCATTTGTCTGTAGGATACAAGTGGAAATTGGTCAATCCAGATGCTGAAATGATTTATCTTCCACATCCATTACCAGTTGATAAGGTTGATATCACGTTCAACAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

484

Amino Acids

55.13

Weight (kDa)

8.79

Isoelectric Point (pI)

28.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 458 2.9e-51 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
AatII GACGTC 1 cut(s) 957
AccB7I CCANNNNNTGG 1 cut(s) 1155
AccII CGCG 1 cut(s) 515
AciI CCGC 4 cut(s) 140, 238, 513, 706
AclWI GGATC 4 cut(s) 295, 703, 1205, 1291
AcoI YGGCCR 3 cut(s) 145, 510, 1080
AcsI RAATTY 4 cut(s) 326, 1192, 1201, 1446
AcuI CTGAAG 2 cut(s) 554, 701
AcyI GRCGYC 1 cut(s) 954
AfaI GTAC 2 cut(s) 194, 248
AfiI CCNNNNNNNGG 5 cut(s) 56, 167, 189, 508, 1155
AgsI TTSAA 4 cut(s) 199, 581, 1225, 1441
AjnI CCWGG 2 cut(s) 563, 1255
AluBI AGCT 7 cut(s) 85, 438, 490, 733, 757, 967, 1250
AluI AGCT 7 cut(s) 85, 438, 490, 733, 757, 967, 1250
Alw26I GTCTC 1 cut(s) 423
AlwI GGATC 4 cut(s) 295, 703, 1205, 1291
AlwNI CAGNNNCTG 1 cut(s) 1385
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 6 cut(s) 145, 240, 365, 510, 617, 1080
ApeKI GCWGC 1 cut(s) 1091
ApoI RAATTY 4 cut(s) 326, 1192, 1201, 1446
ArsI GACNNNNNNTTYG 2 cut(s) 19, 51
Asp700I GAANNNNTTC 1 cut(s) 596
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 240, 366, 617
AsuC2I CCSGG 5 cut(s) 144, 314, 315, 615, 693
AsuHPI GGTGA 6 cut(s) 37, 172, 182, 269, 282, 799
AvaI CYCGRG 1 cut(s) 313
BalI TGGCCA 1 cut(s) 1082
BanII GRGCYC 2 cut(s) 532, 676
BbvI GCAGC 1 cut(s) 1103
BccI CCATC 4 cut(s) 248, 305, 800, 1230
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 888, 922
BciT130I CCWGG 2 cut(s) 565, 1257
BciVI GTATCC 1 cut(s) 1349
BcnI CCSGG 5 cut(s) 144, 314, 315, 615, 693
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 4 cut(s) 359, 390, 758, 863
BfmI CTRYAG 2 cut(s) 396, 1350
BfoI RGCGCY 1 cut(s) 132
BfuI GTATCC 1 cut(s) 1349
BisI GCNGC 3 cut(s) 140, 513, 1092
BlpI GCTNAGC 2 cut(s) 234, 1251
BlsI GCNGC 3 cut(s) 141, 514, 1093
Bme1390I CCNGG 7 cut(s) 144, 314, 315, 565, 615, 693, 1257
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 3 cut(s) 240, 366, 617
BmiI GGNNCC 2 cut(s) 612, 618
BmrFI CCNGG 7 cut(s) 144, 314, 315, 565, 615, 693, 1257
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 2 cut(s) 908, 1372
BmuI ACTGGG 1 cut(s) 1014
Bpu1102I GCTNAGC 2 cut(s) 234, 1251
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 5 cut(s) 144, 314, 315, 615, 693
BsaAI YACGTR 1 cut(s) 1020
BsaBI GATNNNNATC 1 cut(s) 898
BsaHI GRCGYC 1 cut(s) 954
BsaJI CCNNGG 4 cut(s) 30, 313, 614, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 167, 189, 508, 1155
Bse118I RCCGGY 1 cut(s) 125
Bse1I ACTGG 5 cut(s) 368, 655, 667, 1009, 1415
Bse3DI GCAATG 1 cut(s) 1086
Bse8I GATNNNNATC 1 cut(s) 898
BseBI CCWGG 2 cut(s) 565, 1257
BseDI CCNNGG 4 cut(s) 30, 313, 614, 1149
BseGI GGATG 4 cut(s) 354, 676, 811, 1405
BseJI GATNNNNATC 1 cut(s) 898
BseLI CCNNNNNNNGG 5 cut(s) 56, 167, 189, 508, 1155
BseMI GCAATG 1 cut(s) 1086
BseNI ACTGG 5 cut(s) 368, 655, 667, 1009, 1415
BseXI GCAGC 1 cut(s) 1103
BsgI GTGCAG 1 cut(s) 1317
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 6 cut(s) 147, 242, 367, 512, 619, 1082
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 5 cut(s) 126, 144, 314, 614, 693
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 5 cut(s) 56, 167, 189, 508, 1155
BsmAI GTCTC 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 168
BsnI GGCC 6 cut(s) 147, 242, 367, 512, 619, 1082
BsoBI CYCGRG 1 cut(s) 313
Bsp1286I GDGCHC 2 cut(s) 532, 676
Bsp143I GATC 5 cut(s) 287, 695, 796, 1210, 1283
Bsp1720I GCTNAGC 2 cut(s) 234, 1251
BspACI CCGC 4 cut(s) 140, 238, 513, 706
BspANI GGCC 6 cut(s) 147, 242, 367, 512, 619, 1082
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 2 cut(s) 612, 618
BspPI GGATC 4 cut(s) 295, 703, 1205, 1291
BsrDI GCAATG 1 cut(s) 1086
BsrFI RCCGGY 1 cut(s) 125
BsrI ACTGG 5 cut(s) 368, 655, 667, 1009, 1415
BssAI RCCGGY 1 cut(s) 125
BssECI CCNNGG 4 cut(s) 30, 313, 614, 1149
BssMI GATC 5 cut(s) 287, 695, 796, 1210, 1283
BssNI GRCGYC 1 cut(s) 954
BssT1I CCWWGG 2 cut(s) 30, 1149
Bst2UI CCWGG 2 cut(s) 565, 1257
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 969
BstACI GRCGYC 1 cut(s) 954
BstBAI YACGTR 1 cut(s) 1020
BstC8I GCNNGC 3 cut(s) 240, 307, 488
BstDEI CTNAG 3 cut(s) 234, 1116, 1251
BstEII GGTNACC 1 cut(s) 25
BstF5I GGATG 4 cut(s) 354, 676, 811, 1405
BstFNI CGCG 1 cut(s) 515
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 5 cut(s) 290, 698, 799, 1213, 1286
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 5 cut(s) 287, 695, 796, 1210, 1283
BstMWI GCNNNNNNNGC 3 cut(s) 239, 896, 1088
BstNI CCWGG 2 cut(s) 565, 1257
BstNSI RCATGY 1 cut(s) 309
BstPI GGTNACC 1 cut(s) 25
BstSCI CCNGG 7 cut(s) 142, 312, 313, 563, 613, 691, 1255
BstSFI CTRYAG 2 cut(s) 396, 1350
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 1 cut(s) 1103
BstXI CCANNNNNNTGG 2 cut(s) 1236, 1274
BsuI GTATCC 1 cut(s) 1349
BsuRI GGCC 6 cut(s) 147, 242, 367, 512, 619, 1082
BtgZI GCGATG 1 cut(s) 267
BtsCI GGATG 4 cut(s) 354, 676, 811, 1405
BtsIMutI CAGTG 3 cut(s) 660, 680, 725
Cac8I GCNNGC 3 cut(s) 240, 307, 488
CaiI CAGNNNCTG 1 cut(s) 1385
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 125
Cfr13I GGNCC 3 cut(s) 240, 366, 617
Cfr9I CCCGGG 1 cut(s) 313
CseI GACGC 1 cut(s) 476
Csp6I GTAC 2 cut(s) 193, 247
CspCI CAANNNNNGTGG 2 cut(s) 1038, 1073
CviAII CATG 7 cut(s) 151, 306, 410, 525, 710, 992, 1216
CviQI GTAC 2 cut(s) 193, 247
DdeI CTNAG 3 cut(s) 234, 1116, 1251
DpnI GATC 5 cut(s) 289, 697, 798, 1212, 1285
DpnII GATC 5 cut(s) 287, 695, 796, 1210, 1283
DraI TTTAAA 1 cut(s) 1035
EaeI YGGCCR 3 cut(s) 145, 510, 1080
Eam1104I CTCTTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 969
Eco130I CCWWGG 2 cut(s) 30, 1149
Eco24I GRGCYC 2 cut(s) 532, 676
Eco32I GATATC 2 cut(s) 1181, 1432
Eco57I CTGAAG 2 cut(s) 554, 701
Eco88I CYCGRG 1 cut(s) 313
Eco91I GGTNACC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 617
EcoO65I GGTNACC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 563, 1255
EcoRV GATATC 2 cut(s) 1181, 1432
EcoT14I CCWWGG 2 cut(s) 30, 1149
EcoT22I ATGCAT 1 cut(s) 901
EcoT38I GRGCYC 2 cut(s) 532, 676
ErhI CCWWGG 2 cut(s) 30, 1149
FaeI CATG 7 cut(s) 154, 309, 413, 528, 713, 995, 1219
FaqI GGGAC 1 cut(s) 168
FatI CATG 7 cut(s) 150, 305, 409, 524, 709, 991, 1215
FauI CCCGC 1 cut(s) 231
Fnu4HI GCNGC 3 cut(s) 140, 513, 1092
FokI GGATG 4 cut(s) 341, 663, 818, 1392
FriOI GRGCYC 2 cut(s) 532, 676
Fsp4HI GCNGC 3 cut(s) 140, 513, 1092
FspBI CTAG 4 cut(s) 359, 390, 758, 863
GlaI GCGC 1 cut(s) 130
GluI GCNGC 3 cut(s) 140, 513, 1092
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 6 cut(s) 147, 242, 367, 512, 619, 1082
HapII CCGG 5 cut(s) 126, 144, 314, 614, 693
HgaI GACGC 1 cut(s) 476
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 954
Hin1II CATG 7 cut(s) 154, 309, 413, 528, 713, 995, 1219
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 731, 965
HinfI GANTC 4 cut(s) 258, 318, 521, 543
HpaII CCGG 5 cut(s) 126, 144, 314, 614, 693
HphI GGTGA 6 cut(s) 37, 172, 182, 269, 282, 799
Hpy166II GTNNAC 2 cut(s) 648, 924
Hpy188I TCNGA 5 cut(s) 263, 343, 534, 1106, 1198
Hpy188III TCNNGA 6 cut(s) 215, 464, 863, 1287, 1315, 1379
Hpy8I GTNNAC 2 cut(s) 648, 924
Hpy99I CGWCG 2 cut(s) 213, 480
HpyAV CCTTC 7 cut(s) 193, 361, 529, 562, 725, 776, 827
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4IV ACGT 3 cut(s) 954, 1019, 1436
HpyCH4V TGCA 5 cut(s) 817, 899, 1091, 1298, 1328
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 896, 1088
HpyF3I CTNAG 3 cut(s) 234, 1116, 1251
HpySE526I ACGT 3 cut(s) 954, 1019, 1436
Hsp92I GRCGYC 1 cut(s) 954
Hsp92II CATG 7 cut(s) 154, 309, 413, 528, 713, 995, 1219
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 287, 695, 796, 1210, 1283
LmnI GCTCC 2 cut(s) 610, 754
Lsp1109I GCAGC 1 cut(s) 1103
LweI GCATC 2 cut(s) 908, 1372
MaeI CTAG 4 cut(s) 359, 390, 758, 863
MaeII ACGT 3 cut(s) 954, 1019, 1436
MaeIII GTNAC 5 cut(s) 25, 571, 658, 787, 848
MalI GATC 5 cut(s) 289, 697, 798, 1212, 1285
MboI GATC 5 cut(s) 287, 695, 796, 1210, 1283
MboII GAAGA 6 cut(s) 169, 775, 986, 1037, 1076, 1391
MhlI GDGCHC 2 cut(s) 532, 676
MlsI TGGCCA 1 cut(s) 1082
MluNI TGGCCA 1 cut(s) 1082
MlyI GAGTC 3 cut(s) 267, 530, 552
MmeI TCCRAC 3 cut(s) 241, 498, 520
MnlI CCTC 6 cut(s) 111, 290, 630, 730, 850, 968
Mox20I TGGCCA 1 cut(s) 1082
Mph1103I ATGCAT 1 cut(s) 901
MroXI GAANNNNTTC 1 cut(s) 596
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 4 cut(s) 456, 822, 845, 1034
Msp20I TGGCCA 1 cut(s) 1082
MspI CCGG 5 cut(s) 126, 144, 314, 614, 693
MspR9I CCNGG 7 cut(s) 144, 314, 315, 565, 615, 693, 1257
MvaI CCWGG 2 cut(s) 565, 1257
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 3 cut(s) 239, 896, 1088
NciI CCSGG 5 cut(s) 144, 314, 315, 615, 693
NdeII GATC 5 cut(s) 287, 695, 796, 1210, 1283
NlaIII CATG 7 cut(s) 154, 309, 413, 528, 713, 995, 1219
NlaIV GGNNCC 2 cut(s) 612, 618
NmuCI GTSAC 5 cut(s) 25, 571, 658, 787, 848
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 1 cut(s) 309
PaeI GCATGC 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 1155
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 3 cut(s) 141, 514, 1093
PleI GAGTC 3 cut(s) 266, 529, 551
PpsI GAGTC 3 cut(s) 266, 529, 551
Ppu21I YACGTR 1 cut(s) 1020
PsiI TTATAA 1 cut(s) 1140
Psp6I CCWGG 2 cut(s) 563, 1255
PspEI GGTNACC 1 cut(s) 25
PspGI CCWGG 2 cut(s) 563, 1255
PspN4I GGNNCC 2 cut(s) 612, 618
PspPI GGNCC 3 cut(s) 240, 366, 617
PstNI CAGNNNCTG 1 cut(s) 1385
RsaI GTAC 2 cut(s) 194, 248
RsaNI GTAC 2 cut(s) 193, 247
SaqAI TTAA 4 cut(s) 456, 822, 845, 1034
SatI GCNGC 3 cut(s) 140, 513, 1092
Sau3AI GATC 5 cut(s) 287, 695, 796, 1210, 1283
Sau96I GGNCC 3 cut(s) 240, 366, 617
SchI GAGTC 3 cut(s) 267, 530, 552
ScrFI CCNGG 7 cut(s) 144, 314, 315, 565, 615, 693, 1257
SduI GDGCHC 2 cut(s) 532, 676
SfaNI GCATC 2 cut(s) 908, 1372
SfcI CTRYAG 2 cut(s) 396, 1350
SmaI CCCGGG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
SphI GCATGC 1 cut(s) 309
SsiI CCGC 4 cut(s) 140, 238, 513, 706
SspI AATATT 1 cut(s) 586
SspMI CTAG 4 cut(s) 359, 390, 758, 863
StyD4I CCNGG 7 cut(s) 142, 312, 313, 563, 613, 691, 1255
StyI CCWWGG 2 cut(s) 30, 1149
TaaI ACNGT 1 cut(s) 461
TaiI ACGT 3 cut(s) 957, 1022, 1439
TaqI TCGA 2 cut(s) 1177, 1286
TauI GCSGC 2 cut(s) 142, 515
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 4 cut(s) 456, 822, 845, 1034
Tru9I TTAA 4 cut(s) 456, 822, 845, 1034
TscAI CASTG 3 cut(s) 667, 687, 725
TseFI GTSAC 5 cut(s) 25, 571, 658, 787, 848
TseI GCWGC 1 cut(s) 1091
Tsp45I GTSAC 5 cut(s) 25, 571, 658, 787, 848
TspDTI ATGAA 8 cut(s) 343, 360, 398, 573, 846, 1038, 1055, 1257
TspGWI ACGGA 1 cut(s) 464
TspMI CCCGGG 1 cut(s) 313
TspRI CASTG 3 cut(s) 667, 687, 725
Van91I CCANNNNNTGG 1 cut(s) 1155
XapI RAATTY 4 cut(s) 326, 1192, 1201, 1446
XbaI TCTAGA 1 cut(s) 862
XceI RCATGY 1 cut(s) 309
XcmI CCANNNNNNNNNTGG 1 cut(s) 1274
XmaI CCCGGG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 4 cut(s) 359, 390, 758, 863
ZraI GACGTC 1 cut(s) 955
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.