FvH4_5g15600

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004857
Physical Location & Seq
Reverse (-)
8802097 .. 8804169
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15600.t1

Sequence Viewer

Length: 1266 bp
ATGGAGATCTCTTTGGGGGTATGGGTGGCCTTGGTTGTGGGAGGTCTGCCATTTTTGGGGTTGTTGTTGTGGTGGTGGAATGAGTTCTGGTATGTTCTTCCTCTTAAGTTACATGGGTCTCCGACCGGAGCCAAATTGCCTCCAGGTCACTTGGGTTTCCCGTTTCTCGGTGAAATGCTCACTTTCTTGTGGTATTTCAAAACCGTTGGTCGTCCTGATGAGTTTATCAACTCCAAGCGAGCTAAGTATGGTGATGGAGTAGGAATGTACAGAACACACCTGTTCGGAAAGCCATCGATCATAACGTGCTTACCTGCGATCAACAAATTCGTATTCCAGTCGGAGGAGATCTTCCCTCTAAATTGGCCTAATGTTGATGTAGTAGGCTCTAGGCCTCTAGTTCTTTGTTCAGCCACGAATGGTGGCTGCAATCTCAAAGGTAGAATAAAAGCATATGATGAATCTAAGAAGGTAACTTTTGAAAATATTGGAAAATTGTTCGCAAGTTTGGAGCCCGGGCCTCTCCTAGATAACATTGATGAATTGTTTGTGGGAGTGGTCAACGGCATTAGAGCTCAACCTTTCAATGTTCCTGGAACTGCTTATCACCATGCTCTTCAGTGTAGGAGGAAGCTCGAGGCGATTTTCAGGGTGGAGCTGGAGAAGAAAAAGAACTGTAAAGAAGCAGTGACGAGTGATCTGATGGACGGGCTTATGCAAATCAAAGATGATGATGGTGATAAATTAAGTGACAAAGAGGTGATAGATAACATTGTGAGCCTTATTATTGCCGGATATGCTTCCACTGCCCTTTCAGCAATGTGGGCTATCTTCTATCTTGCCAAATACCCCAATGTTTTTCAAAAGCTTCGGGAGGAGAATCTGATTGTAAGTCGGAACAGGAACGGGGAATTCATTACAAGCGACGATGTTTCGAAAATGAAATACACCAACAAAGTGGTGGAAGAAATTATACGGCTGGCCAACATTGCAGCGTTTGTTTTCCGATCAGTTACTAAGGAAATTGAGTATAAAGGCTACATAATACCAAAGAACTGGAATATGATTGTTTGGGTTCGATATCTCCATACAAATCCGGAGCACTTTGATAATCCTATGTGCTTTAATCCAGATAGATGGAATAATCCGGCTAAACCTGGAACATACCAAGTGTTTGGAAGTGGATCAAGAATATGCGCAGGAAATATGCTTGTTAGACTACAGCTCGCAATTTTCTTACACCATTTGTCTGTTGGATACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

422

Amino Acids

47.72

Weight (kDa)

8.86

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 198 - 416 2.4e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1198
Acc36I ACCTGC 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 1096
AclWI GGATC 1 cut(s) 1192
AcoI YGGCCR 1 cut(s) 981
AcsI RAATTY 2 cut(s) 326, 911
AcuI CTGAAG 1 cut(s) 602
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 56, 167, 343
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 199, 482, 586, 863
AjnI CCWGG 3 cut(s) 142, 592, 1156
AluBI AGCT 6 cut(s) 242, 575, 634, 658, 868, 1225
AluI AGCT 6 cut(s) 242, 575, 634, 658, 868, 1225
Alw21I GWGCWC 2 cut(s) 577, 1104
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 1192
Ama87I CYCGRG 2 cut(s) 515, 635
Aor13HI TCCGGA 1 cut(s) 1096
AoxI GGCC 5 cut(s) 27, 365, 392, 518, 981
ApeKI GCWGC 2 cut(s) 426, 992
ApoI RAATTY 2 cut(s) 326, 911
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 1199
AspS9I GGNCC 1 cut(s) 518
AsuC2I CCSGG 2 cut(s) 516, 517
AsuHPI GGTGA 5 cut(s) 182, 263, 599, 749, 772
AsuII TTCGAA 1 cut(s) 935
AvaI CYCGRG 2 cut(s) 515, 635
BalI TGGCCA 1 cut(s) 983
BanII GRGCYC 2 cut(s) 516, 577
BarI GAAGNNNNNNTAC 2 cut(s) 957, 989
Bbv12I GWGCWC 2 cut(s) 577, 1104
BbvI GCAGC 2 cut(s) 413, 1004
BccI CCATC 5 cut(s) 248, 301, 697, 728, 1131
BceAI ACGGC 2 cut(s) 580, 992
BciT130I CCWGG 3 cut(s) 144, 594, 1158
BciVI GTATCC 1 cut(s) 1250
BcnI CCSGG 2 cut(s) 516, 517
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 3 cut(s) 390, 398, 527
BfmI CTRYAG 1 cut(s) 1220
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 322
BfuI GTATCC 1 cut(s) 1250
BglII AGATCT 2 cut(s) 6, 348
BisI GCNGC 2 cut(s) 427, 993
BlsI GCNGC 2 cut(s) 428, 994
Bme1390I CCNGG 5 cut(s) 144, 516, 517, 594, 1158
BmeT110I CYCGRG 2 cut(s) 515, 635
BmgT120I GGNCC 1 cut(s) 518
BmiI GGNNCC 2 cut(s) 130, 513
BmrFI CCNGG 5 cut(s) 144, 516, 517, 594, 1158
BpmI CTGGAG 2 cut(s) 126, 680
Bpu14I TTCGAA 1 cut(s) 935
BpuMI CCSGG 2 cut(s) 516, 517
Bsa29I ATCGAT 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 30, 515
BsaWI WCCGGW 2 cut(s) 125, 1096
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 167, 343
Bse1I ACTGG 2 cut(s) 337, 1061
Bse3DI GCAATG 2 cut(s) 825, 987
BseAI TCCGGA 1 cut(s) 1096
BseBI CCWGG 3 cut(s) 144, 594, 1158
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 30, 515
BseLI CCNNNNNNNGG 3 cut(s) 56, 167, 343
BseMI GCAATG 2 cut(s) 825, 987
BseNI ACTGG 2 cut(s) 337, 1061
BseRI GAGGAG 2 cut(s) 359, 890
BseXI GCAGC 2 cut(s) 413, 1004
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 5 cut(s) 29, 367, 394, 520, 983
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 577, 1104
BsiHKCI CYCGRG 2 cut(s) 515, 635
BsiSI CCGG 5 cut(s) 126, 516, 792, 1097, 1148
BslI CCNNNNNNNGG 3 cut(s) 56, 167, 343
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 5 cut(s) 29, 367, 394, 520, 983
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 2 cut(s) 515, 635
Bsp119I TTCGAA 1 cut(s) 935
Bsp1286I GDGCHC 3 cut(s) 516, 577, 1104
Bsp13I TCCGGA 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
BspANI GGCC 5 cut(s) 29, 367, 394, 520, 983
BspDI ATCGAT 1 cut(s) 296
BspEI TCCGGA 1 cut(s) 1096
BspLI GGNNCC 2 cut(s) 130, 513
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 1 cut(s) 1192
BspQI GCTCTTC 1 cut(s) 621
BspT104I TTCGAA 1 cut(s) 935
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 2 cut(s) 825, 987
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 337, 1061
BssECI CCNNGG 2 cut(s) 30, 515
BssMI GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 3 cut(s) 144, 594, 1158
Bst4CI ACNGT 2 cut(s) 205, 677
Bst6I CTCTTC 1 cut(s) 621
BstAFI CTTAAG 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 935
BstC8I GCNNGC 3 cut(s) 240, 981, 1227
BstDEI CTNAG 3 cut(s) 243, 465, 1017
BstHHI GCGC 1 cut(s) 1199
BstKTI GATC 7 cut(s) 9, 300, 321, 351, 700, 1010, 1187
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 797, 806, 815, 824, 989
BstNI CCWGG 3 cut(s) 144, 594, 1158
BstSCI CCNGG 5 cut(s) 142, 514, 515, 592, 1156
BstSFI CTRYAG 1 cut(s) 1220
BstV1I GCAGC 2 cut(s) 413, 1004
BstX2I RGATCY 2 cut(s) 6, 348
BstXI CCANNNNNNTGG 4 cut(s) 958, 1056, 1137, 1175
BstYI RGATCY 2 cut(s) 6, 348
Bsu15I ATCGAT 1 cut(s) 296
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 5 cut(s) 29, 367, 394, 520, 983
BsuTUI ATCGAT 1 cut(s) 296
BtsI GCAGTG 2 cut(s) 693, 804
BtsIMutI CAGTG 3 cut(s) 626, 693, 804
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 3 cut(s) 240, 981, 1227
CfoI GCGC 1 cut(s) 1199
Cfr13I GGNCC 1 cut(s) 518
Cfr9I CCCGGG 1 cut(s) 515
ClaI ATCGAT 1 cut(s) 296
Csp6I GTAC 1 cut(s) 268
CviAII CATG 2 cut(s) 113, 611
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 243, 465, 1017
DpnI GATC 7 cut(s) 8, 299, 320, 350, 699, 1009, 1186
DpnII GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
Ecl136II GAGCTC 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 394
Eco24I GRGCYC 2 cut(s) 516, 577
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 1082
Eco53kI GAGCTC 1 cut(s) 575
Eco57I CTGAAG 1 cut(s) 602
Eco88I CYCGRG 2 cut(s) 515, 635
EcoICRI GAGCTC 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 911
EcoRII CCWGG 3 cut(s) 142, 592, 1156
EcoRV GATATC 1 cut(s) 1082
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 516, 577
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 116, 614
FatI CATG 2 cut(s) 112, 610
FauNDI CATATG 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 427, 993
FriOI GRGCYC 2 cut(s) 516, 577
Fsp4HI GCNGC 2 cut(s) 427, 993
FspBI CTAG 3 cut(s) 390, 398, 527
FspI TGCGCA 1 cut(s) 1198
GlaI GCGC 1 cut(s) 1198
GluI GCNGC 2 cut(s) 427, 993
GsuI CTGGAG 2 cut(s) 126, 680
HaeIII GGCC 5 cut(s) 29, 367, 394, 520, 983
HapII CCGG 5 cut(s) 126, 516, 792, 1097, 1148
HhaI GCGC 1 cut(s) 1199
Hin1II CATG 2 cut(s) 116, 614
Hin6I GCGC 1 cut(s) 1197
HinP1I GCGC 1 cut(s) 1197
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 866
HinfI GANTC 2 cut(s) 461, 880
HpaII CCGG 5 cut(s) 126, 516, 792, 1097, 1148
HphI GGTGA 5 cut(s) 182, 263, 599, 749, 772
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 7 cut(s) 123, 287, 343, 702, 885, 897, 1007
Hpy188III TCNNGA 5 cut(s) 215, 872, 1097, 1130, 1188
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 1 cut(s) 929
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 205, 677
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 3 cut(s) 429, 718, 992
HpyF10VI GCNNNNNNNGC 5 cut(s) 797, 806, 815, 824, 989
HpyF3I CTNAG 3 cut(s) 243, 465, 1017
HpySE526I ACGT 1 cut(s) 305
Hsp92II CATG 2 cut(s) 116, 614
HspAI GCGC 1 cut(s) 1197
Kpn2I TCCGGA 1 cut(s) 1096
Kzo9I GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 4 cut(s) 128, 511, 655, 1099
Lsp1109I GCAGC 2 cut(s) 413, 1004
MaeI CTAG 3 cut(s) 390, 398, 527
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 6 cut(s) 108, 146, 472, 688, 749, 1012
MalI GATC 7 cut(s) 8, 299, 320, 350, 699, 1009, 1186
MboI GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
MboII GAAGA 6 cut(s) 89, 343, 608, 676, 823, 977
MflI RGATCY 2 cut(s) 6, 348
MhlI GDGCHC 3 cut(s) 516, 577, 1104
MlsI TGGCCA 1 cut(s) 983
MluNI TGGCCA 1 cut(s) 983
MmeI TCCRAC 4 cut(s) 146, 321, 875, 1234
Mox20I TGGCCA 1 cut(s) 983
MroI TCCGGA 1 cut(s) 1096
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 983
MseI TTAA 3 cut(s) 105, 746, 1125
Msp20I TGGCCA 1 cut(s) 983
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 5 cut(s) 126, 516, 792, 1097, 1148
MspR9I CCNGG 5 cut(s) 144, 516, 517, 594, 1158
MvaI CCWGG 3 cut(s) 144, 594, 1158
MwoI GCNNNNNNNGC 5 cut(s) 797, 806, 815, 824, 989
NciI CCSGG 2 cut(s) 516, 517
NdeI CATATG 1 cut(s) 454
NdeII GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
NlaIII CATG 2 cut(s) 116, 614
NlaIV GGNNCC 2 cut(s) 130, 513
NmuCI GTSAC 3 cut(s) 146, 688, 749
NsbI TGCGCA 1 cut(s) 1198
NspV TTCGAA 1 cut(s) 935
PaeR7I CTCGAG 1 cut(s) 635
PceI AGGCCT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 2 cut(s) 461, 880
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 2 cut(s) 428, 994
Psp124BI GAGCTC 1 cut(s) 577
Psp6I CCWGG 3 cut(s) 142, 592, 1156
PspGI CCWGG 3 cut(s) 142, 592, 1156
PspN4I GGNNCC 2 cut(s) 130, 513
PspPI GGNCC 1 cut(s) 518
PspXI VCTCGAGB 1 cut(s) 635
PsuI RGATCY 2 cut(s) 6, 348
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 577
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 3 cut(s) 105, 746, 1125
SatI GCNGC 2 cut(s) 427, 993
Sau3AI GATC 7 cut(s) 6, 297, 318, 348, 697, 1007, 1184
Sau96I GGNCC 1 cut(s) 518
ScrFI CCNGG 5 cut(s) 144, 516, 517, 594, 1158
SduI GDGCHC 3 cut(s) 516, 577, 1104
SfcI CTRYAG 1 cut(s) 1220
Sfr274I CTCGAG 1 cut(s) 635
SfuI TTCGAA 1 cut(s) 935
SlaI CTCGAG 1 cut(s) 635
SmaI CCCGGG 1 cut(s) 517
SmlI CTYRAG 2 cut(s) 104, 635
SmoI CTYRAG 2 cut(s) 104, 635
SrfI GCCCGGGC 1 cut(s) 517
SseBI AGGCCT 1 cut(s) 394
SspI AATATT 1 cut(s) 487
SspMI CTAG 3 cut(s) 390, 398, 527
SstI GAGCTC 1 cut(s) 577
StuI AGGCCT 1 cut(s) 394
StyD4I CCNGG 5 cut(s) 142, 514, 515, 592, 1156
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 205, 677
TaiI ACGT 1 cut(s) 308
TaqI TCGA 4 cut(s) 296, 636, 935, 1078
TatI WGTACW 1 cut(s) 267
TfiI GAWTC 2 cut(s) 461, 880
Tru1I TTAA 3 cut(s) 105, 746, 1125
Tru9I TTAA 3 cut(s) 105, 746, 1125
TscAI CASTG 3 cut(s) 626, 693, 811
TseFI GTSAC 3 cut(s) 146, 688, 749
TseI GCWGC 2 cut(s) 426, 992
Tsp45I GTSAC 3 cut(s) 146, 688, 749
TspDTI ATGAA 4 cut(s) 474, 555, 904, 956
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 3 cut(s) 626, 693, 811
Vha464I CTTAAG 1 cut(s) 104
XapI RAATTY 2 cut(s) 326, 911
XcmI CCANNNNNNNNNTGG 2 cut(s) 958, 1250
XhoI CTCGAG 1 cut(s) 635
XmaI CCCGGG 1 cut(s) 515
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 3 cut(s) 390, 398, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.