FvH4_5g15650

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004857
Physical Location & Seq
Reverse (-)
8837697 .. 8840207
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15650.t1

Sequence Viewer

Length: 1452 bp
ATGGAGATCTCGTTGGGGGTATGGGTGGCATTGGTTGTGGGAGGTCTGCCATTTTTGGGGTTGTTGTTGTGGTGGTGGAATGAGTTCTGGTATGTTCTTCCTCTTAAGTTACGTGGGTCTCCGACCGGAGCCAAATTGCCTCCAGGTCACTTGGGTTTCCCGTTTCTCGGTGAAATGCTCACTTTCTTGTGGTATTTCAAAACCGTTGGTCGTCCTGATGAGTTTATCAACTCCAAGCGAGCTAAGTATGGTGATGGAGTAGGAATGTACAGAACACACCTGTTCGGAAAGCCATCGATCATAACGTGCTTACCTGCGATCAACAAATTCGTATTCCAGTCGGAGGAGATCTTCCCTCTAAATTGGCCTAATGTTGATGTTGTAGGCTCTAGATCTTTGGTAGCAGTTCAAGGAAGCTCCCATGCAAGGCTTAGAACTTATGTCATGAATGTCATTAACAAACCTGATGCTTTGCGCCGCATTGCCGGACTAGTTCAGCCACGAATGGTGGCTGCAATGGAGTTATGGGCACTCCAAGGTAGAATAAAAGCATATGATGAGTCTAAGAAGGTAACTTTTGAAAATATTGGAAAATTGTTCGCAAGTTTGGAGCCCGGGCCTCTCCTAGATACCATTGAAGAATTGTTTGTGGGAGTGGTCAACGGAATTAGAGCTCAACCTTTCAATGTTCCTGGATCTGCTTATCACCATGCTCTTCAGTGTAGGAGGAAACTCGAGGCGATTTTCAGGGTGGAGCTGGAGAAGAAAAAGAACCGTAAAGAAGCAGTGACGACTGATTTGATGGACGGGCTTATGCAAATCACAGATGATGAGGGTGATAAATTAAGTGACAAAGAGGTGATAGATAACATTGTGAGCCTTATTATTGCCGGATATGCTTCCACTGCCCTTTCAGCAATGTGGGCTATCTTCTATCTTGCCAAATACCCCAATGTTTTTCAAAAGCTTCGGGAGGAGAATATGATTGTAAGTAAGAACAGGAACGGGGAATTCATTACAAGTGACGATGTTTCGAAAATGAAATACACCAACAAGGTGGTGGAAGAAATTATACGGCTGGCCAACATTGCAGCGTTTGTTTTCCGATCTGTTACCAAGGAAATTGAGTATAAAGGCTACATAATACCAAAGAACTGGAATATGATTGTTTGGGTTCGATATCTCCATACAAATCCGGAGCACTTTGATGATCCTATGTCCTTTAACCCAGATAGATGGAATAATCCGGCTAAACCTGGAACATACCAAGTGTTTGGAGGTGGATCAAGAATATGTGCTGGAAATATGCTTGTTAGACTCCAACTCGCCATTTTCTTACACTATTTGTCTGTTGGATATAAGTGGAAGTTACTCAACCCGGATGCACCTATGATGTATTTGTCACATCCAAGACCGGTTGATAATGTTGAGGTCACATTCAGCAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

484

Amino Acids

54.98

Weight (kDa)

9.22

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 460 7.3e-52 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 1195
AciI CCGC 1 cut(s) 478
AclWI GGATC 3 cut(s) 703, 1205, 1291
AcoI YGGCCR 1 cut(s) 1080
AcsI RAATTY 3 cut(s) 326, 1010, 1446
AcuI CTGAAG 1 cut(s) 701
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 426
AflII CTTAAG 1 cut(s) 104
AgeI ACCGGT 1 cut(s) 1414
AgsI TTSAA 6 cut(s) 199, 410, 581, 638, 685, 962
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 3 cut(s) 142, 691, 1255
AluBI AGCT 5 cut(s) 242, 417, 674, 757, 967
AluI AGCT 5 cut(s) 242, 417, 674, 757, 967
Alw21I GWGCWC 2 cut(s) 676, 1203
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 3 cut(s) 703, 1205, 1291
Ama87I CYCGRG 2 cut(s) 614, 734
Aor13HI TCCGGA 1 cut(s) 1195
AoxI GGCC 3 cut(s) 365, 617, 1080
ApeKI GCWGC 2 cut(s) 512, 1091
ApoI RAATTY 3 cut(s) 326, 1010, 1446
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AsiGI ACCGGT 1 cut(s) 1414
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 477
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 3 cut(s) 615, 616, 1379
AsuHPI GGTGA 5 cut(s) 182, 263, 698, 848, 871
AsuII TTCGAA 1 cut(s) 1034
AvaI CYCGRG 2 cut(s) 614, 734
BaeGI GKGCMC 1 cut(s) 532
BalI TGGCCA 1 cut(s) 1082
BanII GRGCYC 2 cut(s) 615, 676
BarI GAAGNNNNNNTAC 2 cut(s) 1056, 1088
Bbv12I GWGCWC 2 cut(s) 676, 1203
BbvI GCAGC 2 cut(s) 499, 1103
BccI CCATC 4 cut(s) 248, 301, 796, 1230
BceAI ACGGC 1 cut(s) 1091
BciT130I CCWGG 3 cut(s) 144, 693, 1257
BcnI CCSGG 3 cut(s) 615, 616, 1379
BcoDI GTCTC 1 cut(s) 123
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 3 cut(s) 390, 491, 626
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 322
BglII AGATCT 3 cut(s) 6, 348, 392
BisI GCNGC 3 cut(s) 478, 513, 1092
BlsI GCNGC 3 cut(s) 479, 514, 1093
Bme1390I CCNGG 6 cut(s) 144, 615, 616, 693, 1257, 1379
BmeT110I CYCGRG 2 cut(s) 614, 734
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 2 cut(s) 130, 612
BmrFI CCNGG 6 cut(s) 144, 615, 616, 693, 1257, 1379
BmsI GCATC 2 cut(s) 457, 1372
BpmI CTGGAG 2 cut(s) 126, 779
Bpu14I TTCGAA 1 cut(s) 1034
BpuMI CCSGG 3 cut(s) 615, 616, 1379
Bsa29I ATCGAT 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 113
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 535, 614, 1116
BsaWI WCCGGW 3 cut(s) 125, 1195, 1414
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 167, 343, 426
Bse118I RCCGGY 1 cut(s) 1414
Bse1I ACTGG 2 cut(s) 337, 1160
Bse3DI GCAATG 4 cut(s) 480, 522, 924, 1086
BseAI TCCGGA 1 cut(s) 1195
BseBI CCWGG 3 cut(s) 144, 693, 1257
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 535, 614, 1116
BseGI GGATG 2 cut(s) 1387, 1405
BseLI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 426
BseMI GCAATG 4 cut(s) 480, 522, 924, 1086
BseNI ACTGG 2 cut(s) 337, 1160
BseRI GAGGAG 2 cut(s) 359, 989
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 499, 1103
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 367, 619, 1082
BshTI ACCGGT 1 cut(s) 1414
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 676, 1203
BsiHKCI CYCGRG 2 cut(s) 614, 734
BsiSI CCGG 8 cut(s) 126, 486, 615, 891, 1196, 1247, 1379, 1415
BslI CCNNNNNNNGG 4 cut(s) 56, 167, 343, 426
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 3 cut(s) 367, 619, 1082
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 2 cut(s) 614, 734
Bsp119I TTCGAA 1 cut(s) 1034
Bsp1286I GDGCHC 4 cut(s) 532, 615, 676, 1203
Bsp13I TCCGGA 1 cut(s) 1195
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
BspACI CCGC 1 cut(s) 478
BspANI GGCC 3 cut(s) 367, 619, 1082
BspDI ATCGAT 1 cut(s) 296
BspEI TCCGGA 1 cut(s) 1195
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 130, 612
BspMI ACCTGC 1 cut(s) 322
BspPI GGATC 3 cut(s) 703, 1205, 1291
BspQI GCTCTTC 1 cut(s) 720
BspT104I TTCGAA 1 cut(s) 1034
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 123
BsrDI GCAATG 4 cut(s) 480, 522, 924, 1086
BsrFI RCCGGY 1 cut(s) 1414
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 337, 1160
BssAI RCCGGY 1 cut(s) 1414
BssECI CCNNGG 3 cut(s) 535, 614, 1116
BssMI GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
BssT1I CCWWGG 2 cut(s) 535, 1116
Bst2UI CCWGG 3 cut(s) 144, 693, 1257
Bst4CI ACNGT 2 cut(s) 205, 776
Bst6I CTCTTC 1 cut(s) 720
BstAFI CTTAAG 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 1034
BstC8I GCNNGC 2 cut(s) 240, 1080
BstDEI CTNAG 3 cut(s) 243, 431, 564
BstF5I GGATG 2 cut(s) 1387, 1405
BstHHI GCGC 1 cut(s) 477
BstKTI GATC 9 cut(s) 9, 300, 321, 351, 395, 698, 1109, 1213, 1286
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 896, 905, 914, 923, 1088
BstNI CCWGG 3 cut(s) 144, 693, 1257
BstSCI CCNGG 6 cut(s) 142, 613, 614, 691, 1255, 1377
BstSLI GKGCMC 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 499, 1103
BstX2I RGATCY 4 cut(s) 6, 348, 392, 695
BstXI CCANNNNNNTGG 4 cut(s) 1057, 1155, 1236, 1274
BstYI RGATCY 4 cut(s) 6, 348, 392, 695
Bsu15I ATCGAT 1 cut(s) 296
BsuRI GGCC 3 cut(s) 367, 619, 1082
BsuTUI ATCGAT 1 cut(s) 296
BtsCI GGATG 2 cut(s) 1387, 1405
BtsI GCAGTG 2 cut(s) 792, 903
BtsIMutI CAGTG 3 cut(s) 725, 792, 903
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 2 cut(s) 240, 1080
CciI TCATGA 1 cut(s) 444
CfoI GCGC 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 1414
Cfr13I GGNCC 1 cut(s) 617
Cfr9I CCCGGG 1 cut(s) 614
ClaI ATCGAT 1 cut(s) 296
Csp6I GTAC 1 cut(s) 268
CspAI ACCGGT 1 cut(s) 1414
CviAII CATG 3 cut(s) 422, 445, 710
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 3 cut(s) 243, 431, 564
DpnI GATC 9 cut(s) 8, 299, 320, 350, 394, 697, 1108, 1212, 1285
DpnII GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
EaeI YGGCCR 1 cut(s) 1080
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Ecl136II GAGCTC 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 535, 1116
Eco24I GRGCYC 2 cut(s) 615, 676
Eco31I GGTCTC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 1181
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 701
Eco88I CYCGRG 2 cut(s) 614, 734
EcoICRI GAGCTC 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 1010
EcoRII CCWGG 3 cut(s) 142, 691, 1255
EcoRV GATATC 1 cut(s) 1181
EcoT14I CCWWGG 2 cut(s) 535, 1116
EcoT38I GRGCYC 2 cut(s) 615, 676
ErhI CCWWGG 2 cut(s) 535, 1116
FaeI CATG 3 cut(s) 425, 448, 713
FatI CATG 3 cut(s) 421, 444, 709
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 478, 513, 1092
FokI GGATG 2 cut(s) 1392, 1394
FriOI GRGCYC 2 cut(s) 615, 676
Fsp4HI GCNGC 3 cut(s) 478, 513, 1092
FspBI CTAG 3 cut(s) 390, 491, 626
GlaI GCGC 1 cut(s) 476
GluI GCNGC 3 cut(s) 478, 513, 1092
GsuI CTGGAG 2 cut(s) 126, 779
HaeIII GGCC 3 cut(s) 367, 619, 1082
HapII CCGG 8 cut(s) 126, 486, 615, 891, 1196, 1247, 1379, 1415
HhaI GCGC 1 cut(s) 477
Hin1II CATG 3 cut(s) 425, 448, 713
Hin6I GCGC 1 cut(s) 475
HinP1I GCGC 1 cut(s) 475
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 2 cut(s) 560, 1317
HpaII CCGG 8 cut(s) 126, 486, 615, 891, 1196, 1247, 1379, 1415
HphI GGTGA 5 cut(s) 182, 263, 698, 848, 871
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 4 cut(s) 123, 287, 343, 1106
Hpy188III TCNNGA 6 cut(s) 215, 390, 445, 971, 1196, 1287
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 2 cut(s) 205, 776
HpyCH4IV ACGT 2 cut(s) 112, 305
HpyCH4V TGCA 5 cut(s) 425, 515, 817, 1091, 1385
HpyF10VI GCNNNNNNNGC 5 cut(s) 896, 905, 914, 923, 1088
HpyF3I CTNAG 3 cut(s) 243, 431, 564
HpySE526I ACGT 2 cut(s) 112, 305
Hsp92II CATG 3 cut(s) 425, 448, 713
HspAI GCGC 1 cut(s) 475
Kpn2I TCCGGA 1 cut(s) 1195
Kzo9I GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 5 cut(s) 128, 422, 610, 754, 1198
Lsp1109I GCAGC 2 cut(s) 499, 1103
LweI GCATC 2 cut(s) 457, 1372
MaeI CTAG 3 cut(s) 390, 491, 626
MaeII ACGT 2 cut(s) 112, 305
MalI GATC 9 cut(s) 8, 299, 320, 350, 394, 697, 1108, 1212, 1285
MboI GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
MboII GAAGA 7 cut(s) 89, 343, 650, 707, 775, 922, 1076
MflI RGATCY 4 cut(s) 6, 348, 392, 695
MhlI GDGCHC 4 cut(s) 532, 615, 676, 1203
MlsI TGGCCA 1 cut(s) 1082
MluNI TGGCCA 1 cut(s) 1082
MlyI GAGTC 2 cut(s) 569, 1311
MmeI TCCRAC 4 cut(s) 146, 321, 1333, 1345
Mox20I TGGCCA 1 cut(s) 1082
MroI TCCGGA 1 cut(s) 1195
MroXI GAANNNNTTC 1 cut(s) 83
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 4 cut(s) 105, 456, 845, 1224
MslI CAYNNNNRTG 1 cut(s) 1206
Msp20I TGGCCA 1 cut(s) 1082
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 8 cut(s) 126, 486, 615, 891, 1196, 1247, 1379, 1415
MspR9I CCNGG 6 cut(s) 144, 615, 616, 693, 1257, 1379
MvaI CCWGG 3 cut(s) 144, 693, 1257
MwoI GCNNNNNNNGC 5 cut(s) 896, 905, 914, 923, 1088
NciI CCSGG 3 cut(s) 615, 616, 1379
NdeI CATATG 1 cut(s) 553
NdeII GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
NlaIII CATG 3 cut(s) 425, 448, 713
NlaIV GGNNCC 2 cut(s) 130, 612
NmuCI GTSAC 6 cut(s) 146, 787, 848, 1022, 1401, 1432
NspV TTCGAA 1 cut(s) 1034
PaeR7I CTCGAG 1 cut(s) 734
PagI TCATGA 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 691
PinAI ACCGGT 1 cut(s) 1414
PkrI GCNGC 3 cut(s) 479, 514, 1093
PleI GAGTC 2 cut(s) 568, 1311
PpsI GAGTC 2 cut(s) 568, 1311
Ppu21I YACGTR 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 676
Psp6I CCWGG 3 cut(s) 142, 691, 1255
PspGI CCWGG 3 cut(s) 142, 691, 1255
PspN4I GGNNCC 2 cut(s) 130, 612
PspPI GGNCC 1 cut(s) 617
PspXI VCTCGAGB 1 cut(s) 734
PsuI RGATCY 4 cut(s) 6, 348, 392, 695
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 1206
SacI GAGCTC 1 cut(s) 676
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 4 cut(s) 105, 456, 845, 1224
SatI GCNGC 3 cut(s) 478, 513, 1092
Sau3AI GATC 9 cut(s) 6, 297, 318, 348, 392, 695, 1106, 1210, 1283
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 2 cut(s) 569, 1311
ScrFI CCNGG 6 cut(s) 144, 615, 616, 693, 1257, 1379
SduI GDGCHC 4 cut(s) 532, 615, 676, 1203
SfaNI GCATC 2 cut(s) 457, 1372
Sfr274I CTCGAG 1 cut(s) 734
SfuI TTCGAA 1 cut(s) 1034
SlaI CTCGAG 1 cut(s) 734
SmaI CCCGGG 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 1206
SmlI CTYRAG 2 cut(s) 104, 734
SmoI CTYRAG 2 cut(s) 104, 734
SpeI ACTAGT 1 cut(s) 490
SrfI GCCCGGGC 1 cut(s) 616
SsiI CCGC 1 cut(s) 478
SspI AATATT 1 cut(s) 586
SspMI CTAG 3 cut(s) 390, 491, 626
SstI GAGCTC 1 cut(s) 676
StyD4I CCNGG 6 cut(s) 142, 613, 614, 691, 1255, 1377
StyI CCWWGG 2 cut(s) 535, 1116
TaaI ACNGT 2 cut(s) 205, 776
TaiI ACGT 2 cut(s) 115, 308
TaqI TCGA 4 cut(s) 296, 735, 1034, 1177
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 480
Tru1I TTAA 4 cut(s) 105, 456, 845, 1224
Tru9I TTAA 4 cut(s) 105, 456, 845, 1224
TscAI CASTG 3 cut(s) 725, 792, 910
TseFI GTSAC 6 cut(s) 146, 787, 848, 1022, 1401, 1432
TseI GCWGC 2 cut(s) 512, 1091
Tsp45I GTSAC 6 cut(s) 146, 787, 848, 1022, 1401, 1432
TspDTI ATGAA 3 cut(s) 461, 1003, 1055
TspGWI ACGGA 1 cut(s) 678
TspMI CCCGGG 1 cut(s) 614
TspRI CASTG 3 cut(s) 725, 792, 910
Vha464I CTTAAG 1 cut(s) 104
XapI RAATTY 3 cut(s) 326, 1010, 1446
XbaI TCTAGA 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 1057
XhoI CTCGAG 1 cut(s) 734
XmaI CCCGGG 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 3 cut(s) 390, 491, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.