Rroxscaffold_3G00272840

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004857
Physical Location & Seq
Reverse (-)
64798780 .. 64804031
5252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00272840.1

Sequence Viewer

Length: 303 bp
ATGTACAGAACACACATGTTCGGAAAGCCATCCATCATAACCTGCTTACCGGAGATCAACAAATTAATATTCCAATCAGAGGACACCTTCAAACTAAACTGGCCTAACGTTGATATTGTAGGTTCTAGCTCCTTGGTAGCAGTTCAAGGAAGCTCCCATGCAAGGCTTAGAAGCTATGTCGTAAATGTCATCAACAAACCCGACGCTCTCCGCCGCATTGCGCAACTAGTTCAGCCGCGAATGTTGGCTGCATTTAGTTATGGGCTCTCAAAGGTAGAATCAAAGCATATGACGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

100

Amino Acids

11.29

Weight (kDa)

10.01

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 222
Acc36I ACCTGC 1 cut(s) 50
AccII CGCG 1 cut(s) 238
AciI CCGC 3 cut(s) 211, 214, 236
AclI AACGTT 1 cut(s) 108
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 79, 162
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 2 cut(s) 91, 146
AhlI ACTAGT 1 cut(s) 226
AluBI AGCT 4 cut(s) 129, 153, 174, 298
AluI AGCT 4 cut(s) 129, 153, 174, 298
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 1 cut(s) 248
AseI ATTAAT 1 cut(s) 65
AspLEI GCGC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 235
BccI CCATC 2 cut(s) 37, 41
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 2 cut(s) 126, 227
BfuAI ACCTGC 1 cut(s) 50
BisI GCNGC 3 cut(s) 214, 236, 249
BlsI GCNGC 3 cut(s) 215, 237, 250
BsaJI CCNNGG 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 162
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 79, 162
BseMI GCAATG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 238
BshFI GGCC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 50
BslI CCNNNNNNNGG 2 cut(s) 79, 162
BsnI GGCC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 54
BspACI CCGC 3 cut(s) 211, 214, 236
BspANI GGCC 1 cut(s) 103
BspFNI CGCG 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 216
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 1 cut(s) 54
BssT1I CCWWGG 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 3
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 29
BstFNI CGCG 1 cut(s) 238
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 1 cut(s) 57
BstMBI GATC 1 cut(s) 54
BstNSI RCATGY 1 cut(s) 19
BstUI CGCG 1 cut(s) 238
BstV1I GCAGC 1 cut(s) 235
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 29
BveI ACCTGC 1 cut(s) 50
CfoI GCGC 1 cut(s) 223
CseI GACGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 4
CviAII CATG 2 cut(s) 16, 158
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 167
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 2 cut(s) 19, 161
FaiI YATR 8 cut(s) 17, 38, 159, 177, 261, 288, 290, 301
FatI CATG 2 cut(s) 15, 157
FauNDI CATATG 1 cut(s) 288
Fnu4HI GCNGC 3 cut(s) 214, 236, 249
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 3 cut(s) 214, 236, 249
FspBI CTAG 2 cut(s) 126, 227
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 1 cut(s) 222
GluI GCNGC 3 cut(s) 214, 236, 249
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 223
Hin1II CATG 2 cut(s) 19, 161
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 50
Hpy188I TCNGA 2 cut(s) 23, 79
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 97
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 2 cut(s) 161, 251
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 2 cut(s) 19, 161
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 2 cut(s) 134, 158
LpnPI CCDG 3 cut(s) 55, 63, 85
Lsp1109I GCAGC 1 cut(s) 235
MaeI CTAG 2 cut(s) 126, 227
MaeII ACGT 1 cut(s) 108
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 62
MnlI CCTC 1 cut(s) 73
MseI TTAA 1 cut(s) 65
MspI CCGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 238
NdeI CATATG 1 cut(s) 288
NdeII GATC 1 cut(s) 54
NlaIII CATG 2 cut(s) 19, 161
NsbI TGCGCA 1 cut(s) 222
NspI RCATGY 1 cut(s) 19
PciI ACATGT 1 cut(s) 15
PfeI GAWTC 1 cut(s) 278
PkrI GCNGC 3 cut(s) 215, 237, 250
PscI ACATGT 1 cut(s) 15
PshBI ATTAAT 1 cut(s) 65
Psp1406I AACGTT 1 cut(s) 108
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 3 cut(s) 214, 236, 249
Sau3AI GATC 1 cut(s) 54
SduI GDGCHC 1 cut(s) 267
SetI ASST 9 cut(s) 44, 89, 111, 124, 131, 155, 176, 276, 300
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 1 cut(s) 62
SsiI CCGC 3 cut(s) 211, 214, 236
SspI AATATT 1 cut(s) 69
SspMI CTAG 2 cut(s) 126, 227
StyI CCWWGG 1 cut(s) 132
TaiI ACGT 1 cut(s) 111
TasI AATT 1 cut(s) 62
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 216, 238
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TseI GCWGC 1 cut(s) 248
VspI ATTAAT 1 cut(s) 65
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 126, 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.