RLG00000005262

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004857
Physical Location & Seq
Reverse (-)
66296645 .. 66308566
11922 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005262

Sequence Viewer

Length: 804 bp
ATGGATATTTCTTTCGGAGTGTGCGTGGATCTGGTTCTCGGAGGTCTGCCATTGCTGGGGTTGCTCTTGTGGTGGTGGAATGAGCTTTGGCACGTGCTTCCTCTCAAACTGCGGCATTCATCTACCAACCTACCTCCGGGTCACATGGGGTTTCCGTTCTTCGGTGAAATGCTCACCTTCATTTGGTACTTCAAAGTTCTTCGCCGCCTCGATGACTTTATCAATGCCAAACGGGCCAAGTACGGAGATGGAGTAGGAATGTATAGAACGCACCTCTTCGGATCACCGTCCATCATTACATGCTTCCCGGCGGTCAACAAATTTGTATTGCAATCGGATGACATCTTCACCTTAGAATGGCCTAATGTTGATCTCATGGGTCCAACTTCTCTAGTTGCGGTTAGTGGAAAGTCTCATGCTAGGGTTAGAAGCTACGTCACCAATGTAATAAACCGGCCAGACGCTCTCCGACGCATTGCCCTTCTCGTTCAACCTCGCATTGTCGCTGCACTTGAGTCGTGGGCTCAAAAGGGTAGAATCAAACCTTACGATGAAGCTAACAAGTTAGCATTTGAAAATATTGGACAACTATTCAGCTCAACCCGTCAACATTCCCGGATTTGCTTATCATCATGCTCTTCAGAGATGCCTTTAATTACCTGCAGATCTAAAATTCATGAAGAACAAGCTGAGGGACCAGAGAAGGAGGGGTTCGCGAATCTGTACAACGGCAATGGTGCTGGAAGTTCCAGAGTTTCCTTTATGGTGCAAAGAAATCGATATGCTTTTGTCGCCGGCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

268

Amino Acids

30.03

Weight (kDa)

9.26

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 668
AccII CGCG 1 cut(s) 716
AciI CCGC 5 cut(s) 112, 205, 311, 398, 798
AclWI GGATC 2 cut(s) 36, 289
AcoI YGGCCR 1 cut(s) 455
AcsI RAATTY 2 cut(s) 320, 672
AcuI CTGAAG 1 cut(s) 624
AcvI CACGTG 1 cut(s) 94
AfaI GTAC 3 cut(s) 188, 242, 725
AfiI CCNNNNNNNGG 5 cut(s) 56, 136, 161, 183, 357
AgsI TTSAA 3 cut(s) 193, 491, 575
AluBI AGCT 5 cut(s) 85, 432, 557, 597, 689
AluI AGCT 5 cut(s) 85, 432, 557, 597, 689
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 2 cut(s) 36, 289
AoxI GGCC 3 cut(s) 234, 359, 455
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 2 cut(s) 320, 672
AspS9I GGNCC 3 cut(s) 234, 380, 695
AsuC2I CCSGG 3 cut(s) 138, 308, 616
AsuHPI GGTGA 5 cut(s) 166, 176, 276, 340, 430
AvaII GGWCC 2 cut(s) 380, 695
BanII GRGCYC 1 cut(s) 526
BbrPI CACGTG 1 cut(s) 94
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 1 cut(s) 493
BccI CCATC 2 cut(s) 242, 299
BceAI ACGGC 1 cut(s) 745
BcgI CGANNNNNNTGC 4 cut(s) 498, 532, 758, 792
BcnI CCSGG 3 cut(s) 138, 308, 616
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 2 cut(s) 392, 420
BfmI CTRYAG 1 cut(s) 661
BfuAI ACCTGC 1 cut(s) 668
BglI GCCNNNNNGGC 1 cut(s) 233
BglII AGATCT 1 cut(s) 665
BisI GCNGC 3 cut(s) 113, 205, 507
BlsI GCNGC 3 cut(s) 114, 206, 508
Bme1390I CCNGG 3 cut(s) 138, 308, 616
Bme18I GGWCC 2 cut(s) 380, 695
BmgT120I GGNCC 3 cut(s) 234, 380, 695
BmiI GGNNCC 2 cut(s) 381, 696
BmrFI CCNGG 3 cut(s) 138, 308, 616
BmsI GCATC 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 690
BpuEI CTTGAG 1 cut(s) 533
BpuMI CCSGG 3 cut(s) 138, 308, 616
Bsa29I ATCGAT 1 cut(s) 778
BsaAI YACGTR 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 237, 267
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 136, 161, 183, 357
Bse118I RCCGGY 2 cut(s) 453, 794
Bse3DI GCAATG 3 cut(s) 50, 474, 739
BseCI ATCGAT 1 cut(s) 778
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 5 cut(s) 56, 136, 161, 183, 357
BseMI GCAATG 3 cut(s) 50, 474, 739
BseMII CTCAG 1 cut(s) 681
BseXI GCAGC 1 cut(s) 493
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 3 cut(s) 236, 361, 457
BshVI ATCGAT 1 cut(s) 778
BsiSI CCGG 5 cut(s) 137, 308, 454, 616, 795
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 5 cut(s) 56, 136, 161, 183, 357
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 1 cut(s) 115
BsnI GGCC 3 cut(s) 236, 361, 457
Bsp1286I GDGCHC 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 28, 281, 370, 665
Bsp68I TCGCGA 1 cut(s) 716
BspACI CCGC 5 cut(s) 112, 205, 311, 398, 798
BspANI GGCC 3 cut(s) 236, 361, 457
BspCNI CTCAG 1 cut(s) 682
BspDI ATCGAT 1 cut(s) 778
BspFNI CGCG 1 cut(s) 716
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 381, 696
BspMAI CTGCAG 1 cut(s) 665
BspMI ACCTGC 1 cut(s) 668
BspPI GGATC 2 cut(s) 36, 289
BspQI GCTCTTC 1 cut(s) 643
BsrDI GCAATG 3 cut(s) 50, 474, 739
BsrFI RCCGGY 2 cut(s) 453, 794
BsrGI TGTACA 1 cut(s) 723
BssAI RCCGGY 2 cut(s) 453, 794
BssMI GATC 4 cut(s) 28, 281, 370, 665
Bst4CI ACNGT 1 cut(s) 288
Bst6I CTCTTC 2 cut(s) 281, 643
BstAUI TGTACA 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 94
BstC8I GCNNGC 1 cut(s) 796
BstDEI CTNAG 2 cut(s) 352, 690
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 716
BstKTI GATC 4 cut(s) 31, 284, 373, 668
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 4 cut(s) 28, 281, 370, 665
BstMWI GCNNNNNNNGC 3 cut(s) 61, 233, 791
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 3 cut(s) 136, 306, 614
BstSFI CTRYAG 1 cut(s) 661
BstUI CGCG 1 cut(s) 716
BstV1I GCAGC 1 cut(s) 493
BstX2I RGATCY 2 cut(s) 28, 665
BstYI RGATCY 2 cut(s) 28, 665
Bsu15I ATCGAT 1 cut(s) 778
BsuRI GGCC 3 cut(s) 236, 361, 457
BsuTUI ATCGAT 1 cut(s) 778
BtsCI GGATG 1 cut(s) 343
BtuMI TCGCGA 1 cut(s) 716
BveI ACCTGC 1 cut(s) 668
Cac8I GCNNGC 1 cut(s) 796
CciI TCATGA 1 cut(s) 676
Cfr10I RCCGGY 2 cut(s) 453, 794
Cfr13I GGNCC 3 cut(s) 234, 380, 695
ClaI ATCGAT 1 cut(s) 778
CseI GACGC 2 cut(s) 470, 480
Csp6I GTAC 3 cut(s) 187, 241, 724
CviAII CATG 6 cut(s) 145, 300, 376, 416, 633, 677
CviJI RGCY 9 cut(s) 85, 236, 361, 432, 457, 524, 557, 597, 689
CviKI_1 RGCY 9 cut(s) 85, 236, 361, 432, 457, 524, 557, 597, 689
CviQI GTAC 3 cut(s) 187, 241, 724
DdeI CTNAG 2 cut(s) 352, 690
DpnI GATC 4 cut(s) 30, 283, 372, 667
DpnII GATC 4 cut(s) 28, 281, 370, 665
EaeI YGGCCR 1 cut(s) 455
Eam1104I CTCTTC 2 cut(s) 281, 643
EarI CTCTTC 2 cut(s) 281, 643
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 2 cut(s) 380, 695
Eco57I CTGAAG 1 cut(s) 624
Eco72I CACGTG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 526
FaeI CATG 6 cut(s) 148, 303, 379, 419, 636, 680
FaiI YATR 9 cut(s) 146, 264, 301, 377, 417, 634, 678, 764, 783
FaqI GGGAC 1 cut(s) 708
FatI CATG 6 cut(s) 144, 299, 375, 415, 632, 676
Fnu4HI GCNGC 3 cut(s) 113, 205, 507
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 3 cut(s) 113, 205, 507
FspBI CTAG 2 cut(s) 392, 420
GluI GCNGC 3 cut(s) 113, 205, 507
GsaI CCCAGC 1 cut(s) 59
HaeIII GGCC 3 cut(s) 236, 361, 457
HapII CCGG 5 cut(s) 137, 308, 454, 616, 795
HgaI GACGC 2 cut(s) 470, 480
Hin1II CATG 6 cut(s) 148, 303, 379, 419, 636, 680
HincII GTYRAC 2 cut(s) 316, 608
HindII GTYRAC 2 cut(s) 316, 608
HinfI GANTC 3 cut(s) 515, 537, 718
HpaII CCGG 5 cut(s) 137, 308, 454, 616, 795
HphI GGTGA 5 cut(s) 166, 176, 276, 340, 430
Hpy166II GTNNAC 2 cut(s) 316, 608
Hpy188I TCNGA 6 cut(s) 17, 41, 281, 337, 470, 643
Hpy188III TCNNGA 3 cut(s) 677, 715, 750
Hpy8I GTNNAC 2 cut(s) 316, 608
Hpy99I CGWCG 1 cut(s) 474
HpyAV CCTTC 3 cut(s) 187, 491, 697
HpyCH4III ACNGT 1 cut(s) 288
HpyCH4IV ACGT 2 cut(s) 93, 435
HpyCH4V TGCA 4 cut(s) 331, 509, 663, 769
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 233, 791
HpyF3I CTNAG 2 cut(s) 352, 690
HpySE526I ACGT 2 cut(s) 93, 435
Hsp92II CATG 6 cut(s) 148, 303, 379, 419, 636, 680
KroI GCCGGC 1 cut(s) 794
KroNI GCCGGC 1 cut(s) 796
Kzo9I GATC 4 cut(s) 28, 281, 370, 665
LguI GCTCTTC 1 cut(s) 643
Lsp1109I GCAGC 1 cut(s) 493
LweI GCATC 1 cut(s) 636
MaeI CTAG 2 cut(s) 392, 420
MaeII ACGT 2 cut(s) 93, 435
MaeIII GTNAC 2 cut(s) 140, 436
MalI GATC 4 cut(s) 30, 283, 372, 667
MboI GATC 4 cut(s) 28, 281, 370, 665
MboII GAAGA 6 cut(s) 151, 191, 268, 337, 630, 692
MflI RGATCY 2 cut(s) 28, 665
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 3 cut(s) 320, 654, 672
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 407, 493
MnlI CCTC 8 cut(s) 35, 111, 144, 218, 284, 504, 685, 700
MreI CGCCGGCG 1 cut(s) 794
MroNI GCCGGC 1 cut(s) 794
MseI TTAA 1 cut(s) 653
MspI CCGG 5 cut(s) 137, 308, 454, 616, 795
MspR9I CCNGG 3 cut(s) 138, 308, 616
Mva1269I GAATGC 1 cut(s) 115
MvnI CGCG 1 cut(s) 716
MwoI GCNNNNNNNGC 3 cut(s) 61, 233, 791
NaeI GCCGGC 1 cut(s) 796
NciI CCSGG 3 cut(s) 138, 308, 616
NdeII GATC 4 cut(s) 28, 281, 370, 665
NgoMIV GCCGGC 1 cut(s) 794
NlaIII CATG 6 cut(s) 148, 303, 379, 419, 636, 680
NlaIV GGNNCC 2 cut(s) 381, 696
NmuCI GTSAC 2 cut(s) 140, 436
NruI TCGCGA 1 cut(s) 716
NspI RCATGY 1 cut(s) 303
PagI TCATGA 1 cut(s) 676
PciSI GCTCTTC 1 cut(s) 643
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PctI GAATGC 1 cut(s) 115
PdiI GCCGGC 1 cut(s) 796
PfeI GAWTC 2 cut(s) 537, 718
PfoI TCCNGGA 1 cut(s) 614
PkrI GCNGC 3 cut(s) 114, 206, 508
PleI GAGTC 1 cut(s) 523
PmaCI CACGTG 1 cut(s) 94
PmlI CACGTG 1 cut(s) 94
PpsI GAGTC 1 cut(s) 523
Ppu21I YACGTR 1 cut(s) 94
PspCI CACGTG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 381, 696
PspPI GGNCC 3 cut(s) 234, 380, 695
PstI CTGCAG 1 cut(s) 665
PsuI RGATCY 2 cut(s) 28, 665
RruI TCGCGA 1 cut(s) 716
RsaI GTAC 3 cut(s) 188, 242, 725
RsaNI GTAC 3 cut(s) 187, 241, 724
SapI GCTCTTC 1 cut(s) 643
SaqAI TTAA 1 cut(s) 653
SatI GCNGC 3 cut(s) 113, 205, 507
Sau3AI GATC 4 cut(s) 28, 281, 370, 665
Sau96I GGNCC 3 cut(s) 234, 380, 695
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 3 cut(s) 138, 308, 616
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 1 cut(s) 636
SfcI CTRYAG 1 cut(s) 661
SgrAI CRCCGGYG 1 cut(s) 794
SinI GGWCC 2 cut(s) 380, 695
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 3 cut(s) 320, 654, 672
SsiI CCGC 5 cut(s) 112, 205, 311, 398, 798
SspI AATATT 1 cut(s) 580
SspMI CTAG 2 cut(s) 392, 420
StyD4I CCNGG 3 cut(s) 136, 306, 614
TaaI ACNGT 1 cut(s) 288
TaiI ACGT 2 cut(s) 96, 438
TaqI TCGA 2 cut(s) 210, 778
TasI AATT 3 cut(s) 320, 654, 672
TatI WGTACW 1 cut(s) 723
TauI GCSGC 2 cut(s) 115, 207
TfiI GAWTC 2 cut(s) 537, 718
Tru1I TTAA 1 cut(s) 653
Tru9I TTAA 1 cut(s) 653
TseFI GTSAC 2 cut(s) 140, 436
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 2 cut(s) 140, 436
TspDTI ATGAA 5 cut(s) 108, 169, 567, 665, 693
TspGWI ACGGA 2 cut(s) 144, 258
VpaK11BI GGWCC 2 cut(s) 380, 695
XapI RAATTY 2 cut(s) 320, 672
XceI RCATGY 1 cut(s) 303
XspI CTAG 2 cut(s) 392, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.