FvH4_5g24800

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005271
Physical Location & Seq
Reverse (-)
16134164 .. 16139603
5440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24800.t1

Sequence Viewer

Length: 720 bp
ATGAAATCCCCTTCTCCCATCTTCAGTTTGATACTCTGTGCTCTCATAAGTCTGTCAACAACTATTCTAACCGCTCAAGGAAATCCTGTATCCTTCTACATCGCTTATCAGTGGCCAGGGTCATACTGTGCAGCAGCAAAGCAGGGTTGTTGCTACCCAAGGAGTATCAGAAAGCATCCCAGTTTCACAATCGGTGGCATATGGGCTTATACGTTTTCTGGGGATATGCCAACCTACTGCAAATCTAAGACCCCTTTTAGTTTGTCCAAGATCTCAAACCTGACAAGGAATTTGGAAAGGAATTGGCCATCACTATCCTGCCCAAGCCATACTAGCAGCACTAGCAGTGTGAAACTCTGGCTGCAAGAATGGGAGAAATACGAGACTTGTGCAGAATCACTGTTTGGAGGTCAATACCAATACTTTCATGCAGCTCTCAATCTCAGAAAGAGAGTGGGCATGCTCCAGATGCTCAGCAATGCAGATATCCATCCAAACGGGTCATTCTACAACGTCACAGCATTTTCTAGTGCCATACAGAAGGCCACCATATATCTTCCAGGGTTCACCTGTAACAAAGACAAATCAGGCAACAAACAGCTCTACCAGATTATACTTTGCGGAGACACAGCCGGAACCAAAATCACAGACTGCCTTGGCTTCCCAAGATCGAATACTGGACAATGCCCTCAAAGATTTAAGTTCCCTCCCTTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

240

Amino Acids

26.6

Weight (kDa)

9.37

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 32 - 218 1.7e-42 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 2 cut(s) 72, 621
AcoI YGGCCR 2 cut(s) 113, 305
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 7
AgsI TTSAA 1 cut(s) 715
AjnI CCWGG 2 cut(s) 115, 559
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 2 cut(s) 434, 601
AluI AGCT 2 cut(s) 434, 601
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 2 cut(s) 377, 618
AoxI GGCC 3 cut(s) 113, 305, 543
ApeKI GCWGC 5 cut(s) 131, 134, 336, 361, 431
ApoI RAATTY 1 cut(s) 289
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
AsuHPI GGTGA 1 cut(s) 559
BalI TGGCCA 2 cut(s) 115, 307
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 5 cut(s) 143, 146, 348, 348, 443
BccI CCATC 3 cut(s) 26, 316, 498
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 2 cut(s) 117, 561
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 377, 618
BfaI CTAG 3 cut(s) 333, 342, 528
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 270
BisI GCNGC 5 cut(s) 132, 135, 337, 362, 432
BlpI GCTNAGC 1 cut(s) 473
BlsI GCNGC 5 cut(s) 133, 136, 338, 363, 433
Bme1390I CCNGG 2 cut(s) 117, 561
BmiI GGNNCC 1 cut(s) 637
BmrFI CCNGG 2 cut(s) 117, 561
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 2 cut(s) 184, 459
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 449
Bpu1102I GCTNAGC 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 489
BsaJI CCNNGG 4 cut(s) 116, 158, 560, 655
Bse1I ACTGG 2 cut(s) 180, 682
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 2 cut(s) 117, 561
BseDI CCNNGG 4 cut(s) 116, 158, 560, 655
BseGI GGATG 2 cut(s) 175, 490
BseJI GATNNNNATC 1 cut(s) 489
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 457, 487
BseNI ACTGG 2 cut(s) 180, 682
BseXI GCAGC 5 cut(s) 143, 146, 348, 348, 443
BsgI GTGCAG 2 cut(s) 150, 411
BshFI GGCC 3 cut(s) 115, 307, 545
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 1 cut(s) 633
BsmAI GTCTC 2 cut(s) 377, 618
BsnI GGCC 3 cut(s) 115, 307, 545
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 2 cut(s) 270, 668
Bsp1720I GCTNAGC 1 cut(s) 473
BspACI CCGC 2 cut(s) 72, 621
BspANI GGCC 3 cut(s) 115, 307, 545
BspCNI CTCAG 2 cut(s) 456, 486
BspLI GGNNCC 1 cut(s) 637
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 484
BsrI ACTGG 2 cut(s) 180, 682
BssECI CCNNGG 4 cut(s) 116, 158, 560, 655
BssMI GATC 2 cut(s) 270, 668
BssT1I CCWWGG 2 cut(s) 158, 655
Bst2UI CCWGG 2 cut(s) 117, 561
Bst4CI ACNGT 2 cut(s) 128, 402
BstC8I GCNNGC 1 cut(s) 461
BstDEI CTNAG 3 cut(s) 246, 443, 473
BstF5I GGATG 2 cut(s) 175, 490
BstKTI GATC 2 cut(s) 273, 671
BstMAI GTCTC 2 cut(s) 377, 618
BstMBI GATC 2 cut(s) 270, 668
BstMWI GCNNNNNNNGC 3 cut(s) 333, 342, 469
BstNI CCWGG 2 cut(s) 117, 561
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 2 cut(s) 115, 559
BstV1I GCAGC 5 cut(s) 143, 146, 348, 348, 443
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 3 cut(s) 115, 307, 545
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 175, 490
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 116, 352, 398
Cac8I GCNNGC 1 cut(s) 461
CviAII CATG 2 cut(s) 428, 460
DdeI CTNAG 3 cut(s) 246, 443, 473
DpnI GATC 2 cut(s) 272, 670
DpnII GATC 2 cut(s) 270, 668
EaeI YGGCCR 2 cut(s) 113, 305
Eco130I CCWWGG 2 cut(s) 158, 655
Eco32I GATATC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 115, 559
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 2 cut(s) 158, 655
ErhI CCWWGG 2 cut(s) 158, 655
FaeI CATG 2 cut(s) 431, 463
FatI CATG 2 cut(s) 427, 459
FauNDI CATATG 1 cut(s) 200
Fnu4HI GCNGC 5 cut(s) 132, 135, 337, 362, 432
FokI GGATG 2 cut(s) 162, 477
Fsp4HI GCNGC 5 cut(s) 132, 135, 337, 362, 432
FspBI CTAG 3 cut(s) 333, 342, 528
GluI GCNGC 5 cut(s) 132, 135, 337, 362, 432
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 3 cut(s) 115, 307, 545
HapII CCGG 1 cut(s) 633
Hin1II CATG 2 cut(s) 431, 463
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 1 cut(s) 395
HpaII CCGG 1 cut(s) 633
HphI GGTGA 1 cut(s) 559
Hpy166II GTNNAC 2 cut(s) 57, 567
Hpy188I TCNGA 2 cut(s) 170, 446
Hpy188III TCNNGA 1 cut(s) 466
Hpy8I GTNNAC 2 cut(s) 57, 567
HpyAV CCTTC 3 cut(s) 21, 103, 535
HpyCH4III ACNGT 2 cut(s) 128, 402
HpyCH4IV ACGT 2 cut(s) 212, 513
HpyCH4V TGCA 6 cut(s) 131, 240, 364, 392, 431, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 333, 342, 469
HpyF3I CTNAG 3 cut(s) 246, 443, 473
HpySE526I ACGT 2 cut(s) 212, 513
Hsp92II CATG 2 cut(s) 431, 463
Kzo9I GATC 2 cut(s) 270, 668
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 5 cut(s) 143, 146, 348, 348, 443
LweI GCATC 2 cut(s) 184, 459
MaeI CTAG 3 cut(s) 333, 342, 528
MaeII ACGT 2 cut(s) 212, 513
MaeIII GTNAC 2 cut(s) 514, 572
MalI GATC 2 cut(s) 272, 670
MbiI CCGCTC 1 cut(s) 74
MboI GATC 2 cut(s) 270, 668
MboII GAAGA 2 cut(s) 13, 548
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 2 cut(s) 115, 307
MluCI AATT 2 cut(s) 289, 301
MluNI TGGCCA 2 cut(s) 115, 307
MnlI CCTC 3 cut(s) 401, 699, 717
Mox20I TGGCCA 2 cut(s) 115, 307
MscI TGGCCA 2 cut(s) 115, 307
MseI TTAA 1 cut(s) 699
Msp20I TGGCCA 2 cut(s) 115, 307
MspI CCGG 1 cut(s) 633
MspR9I CCNGG 2 cut(s) 117, 561
MvaI CCWGG 2 cut(s) 117, 561
MwoI GCNNNNNNNGC 3 cut(s) 333, 342, 469
NdeI CATATG 1 cut(s) 200
NdeII GATC 2 cut(s) 270, 668
NlaIII CATG 2 cut(s) 431, 463
NlaIV GGNNCC 1 cut(s) 637
NmuCI GTSAC 1 cut(s) 514
NspI RCATGY 1 cut(s) 463
PaeI GCATGC 1 cut(s) 463
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 5 cut(s) 133, 136, 338, 363, 433
Psp6I CCWGG 2 cut(s) 115, 559
PspGI CCWGG 2 cut(s) 115, 559
PspN4I GGNNCC 1 cut(s) 637
PsuI RGATCY 1 cut(s) 270
SaqAI TTAA 1 cut(s) 699
SatI GCNGC 5 cut(s) 132, 135, 337, 362, 432
Sau3AI GATC 2 cut(s) 270, 668
ScrFI CCNGG 2 cut(s) 117, 561
SduI GDGCHC 1 cut(s) 43
SetI ASST 8 cut(s) 215, 236, 282, 412, 436, 516, 572, 603
SfaNI GCATC 2 cut(s) 184, 459
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
SphI GCATGC 1 cut(s) 463
Sse9I AATT 2 cut(s) 289, 301
SsiI CCGC 2 cut(s) 72, 621
SspMI CTAG 3 cut(s) 333, 342, 528
StyD4I CCNGG 2 cut(s) 115, 559
StyI CCWWGG 2 cut(s) 158, 655
TaaI ACNGT 2 cut(s) 128, 402
TaiI ACGT 2 cut(s) 215, 516
TaqI TCGA 1 cut(s) 671
TasI AATT 2 cut(s) 289, 301
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 1 cut(s) 699
Tru9I TTAA 1 cut(s) 699
TscAI CASTG 3 cut(s) 116, 352, 405
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 5 cut(s) 131, 134, 336, 361, 431
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 2 cut(s) 17, 416
TspRI CASTG 3 cut(s) 116, 352, 405
XapI RAATTY 1 cut(s) 289
XceI RCATGY 1 cut(s) 463
XspI CTAG 3 cut(s) 333, 342, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.