RchiOBHm_Chr7g0215101

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005271
Physical Location & Seq
Reverse (-)
32554023 .. 32554559
537 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19247

Sequence Viewer

Length: 339 bp
ATGAAATCCCATTCCCCCATCTTCTGTTTGATACTCTCTGCTCTCATAAGCCTGTCAACAACTATTCTAACCGCTCAAGAAAATCCTGTTTCCTTCTACATTGCTTATCAGTGGCCAGGCTCATACTGTGCAGCAGCAAAGCAGGGTTGTTGCTACCCAAAGAGTATTAGAAAACATCCCAGTTTCACTATTGGTGGCATATGGCCTTACACTTTTTCTGGGGATAGACCAACCTACTGCAAATCTAAGACCCCTTTTAGTTTATCTAAGATATCAAACCTGACCAAGAGTTTGGAAAGGAATTGGCCGGCCATCACTATCCTGCCCAAGCCGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

112

Amino Acids

12.53

Weight (kDa)

9.54

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 32 - 105 1.2e-15 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 1 cut(s) 72
AcoI YGGCCR 3 cut(s) 113, 305, 309
AfaI GTAC 1 cut(s) 335
AjnI CCWGG 1 cut(s) 115
AoxI GGCC 4 cut(s) 113, 203, 305, 309
ApeKI GCWGC 2 cut(s) 131, 134
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
BalI TGGCCA 1 cut(s) 115
BbvI GCAGC 2 cut(s) 143, 146
BccI CCATC 2 cut(s) 26, 320
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 117
BfaI CTAG 1 cut(s) 337
BisI GCNGC 2 cut(s) 132, 135
BlsI GCNGC 2 cut(s) 133, 136
Bme1390I CCNGG 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 117
BmrI ACTGGG 1 cut(s) 174
BmuI ACTGGG 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 60
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 180
BseXI GCAGC 2 cut(s) 143, 146
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 4 cut(s) 115, 205, 307, 311
BsiSI CCGG 1 cut(s) 308
BsnI GGCC 4 cut(s) 115, 205, 307, 311
BspACI CCGC 1 cut(s) 72
BspANI GGCC 4 cut(s) 115, 205, 307, 311
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 180
BssAI RCCGGY 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 246, 267
BstF5I GGATG 1 cut(s) 175
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 143, 146
BstXI CCANNNNNNTGG 1 cut(s) 292
BsuRI GGCC 4 cut(s) 115, 205, 307, 311
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 309
Cfr10I RCCGGY 1 cut(s) 307
Csp6I GTAC 1 cut(s) 334
CviJI RGCY 7 cut(s) 51, 115, 120, 205, 307, 311, 331
CviKI_1 RGCY 7 cut(s) 51, 115, 120, 205, 307, 311, 331
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 2 cut(s) 246, 267
EaeI YGGCCR 3 cut(s) 113, 305, 309
Eco32I GATATC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 115
EcoRV GATATC 1 cut(s) 273
FaiI YATR 4 cut(s) 47, 124, 200, 202
FauNDI CATATG 1 cut(s) 200
Fnu4HI GCNGC 2 cut(s) 132, 135
FokI GGATG 1 cut(s) 162
FseI GGCCGGCC 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 132, 135
FspBI CTAG 1 cut(s) 337
GluI GCNGC 2 cut(s) 132, 135
HaeIII GGCC 4 cut(s) 115, 205, 307, 311
HapII CCGG 1 cut(s) 308
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HpaII CCGG 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 131, 240
HpyF3I CTNAG 2 cut(s) 246, 267
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Lsp1109I GCAGC 2 cut(s) 143, 146
MaeI CTAG 1 cut(s) 337
MbiI CCGCTC 1 cut(s) 74
MboII GAAGA 1 cut(s) 13
MlsI TGGCCA 1 cut(s) 115
MluCI AATT 1 cut(s) 301
MluNI TGGCCA 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 115
MroNI GCCGGC 1 cut(s) 307
MscI TGGCCA 1 cut(s) 115
Msp20I TGGCCA 1 cut(s) 115
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
NaeI GCCGGC 1 cut(s) 309
NdeI CATATG 1 cut(s) 200
NgoMIV GCCGGC 1 cut(s) 307
PdiI GCCGGC 1 cut(s) 309
PkrI GCNGC 2 cut(s) 133, 136
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
RigI GGCCGGCC 1 cut(s) 311
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SatI GCNGC 2 cut(s) 132, 135
ScrFI CCNGG 1 cut(s) 117
SetI ASST 2 cut(s) 236, 282
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 1 cut(s) 301
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 128
TasI AATT 1 cut(s) 301
TscAI CASTG 1 cut(s) 116
TseI GCWGC 2 cut(s) 131, 134
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 116
XspI CTAG 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.