Rh7BG278700

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005271
Physical Location & Seq
Reverse (-)
25915356 .. 25916771
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG278700.1

Sequence Viewer

Length: 693 bp
ATGTCCAAGTTCCAACTGGCTCTTCAACCCAAATATCTCTTACAGATATCAAAAATCCTGTTTCCTTCTACATTGCTTATCAGTGGCCAGGCTCATACTGTGCAGCAGCAAAGCAGGGTTGTTGCTACCCAAAGAGTATTAGAAAACATCCCAGTTTCACAATTGGTGGCATATGGCCTTATACTTTTTTCTGGGGATAGACCAACCTACTGCAAATCTAAGACCCCTTTTAGTTTATCCAAGATATCAAACCTGACCAAGAGTTTGGAAAGGAATTGGCCATCACTATCCTGCCCAAGCCGTACTAGTAGCATTAGCAGTAAGAAACTATGGATGCAAGAATGGCAGAAATATGGGACTTGTGCAGAATCACTCTTTGGAGGTCAATACCAATACTTTCATGCAGGTCTCAATCTGAGGAAGAAAATAGACATGCTCCAGATACTCAGCAATGCAGGAATCCATCCAAATGGGTCATTTTATGAGATGACTGCAGTTTTCAATGCCATACAAAAGGGCACCATCTATCTTCCGGGGTTTATCTGTAACAAAGACAAATCAGGCAACAAACAGCTCTACCAGATTATACTCTGTGGAAACACCGCCGGAACCAGAATCACAGACTGCCTCGGCAACCCAAGATCGAATACTGGACAATGCCCCGAAAACTTTAAGTTCCCTCCCTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

230

Amino Acids

25.74

Weight (kDa)

9.66

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 66 - 209 1.1e-33 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 518
AciI CCGC 1 cut(s) 603
AcoI YGGCCR 2 cut(s) 85, 278
AfaI GTAC 1 cut(s) 304
AgsI TTSAA 2 cut(s) 26, 502
AhlI ACTAGT 1 cut(s) 305
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 3 cut(s) 38, 360, 392
AluBI AGCT 1 cut(s) 574
AluI AGCT 1 cut(s) 574
Alw26I GTCTC 1 cut(s) 413
AoxI GGCC 3 cut(s) 85, 175, 278
ApeKI GCWGC 2 cut(s) 103, 106
ArsI GACNNNNNNTTYG 2 cut(s) 247, 279
AsuC2I CCSGG 1 cut(s) 534
BaeGI GKGCMC 1 cut(s) 521
BalI TGGCCA 2 cut(s) 87, 280
BanI GGYRCC 1 cut(s) 518
BbvI GCAGC 2 cut(s) 115, 118
BccI CCATC 3 cut(s) 289, 471, 530
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 413
BcuI ACTAGT 1 cut(s) 305
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 395
BisI GCNGC 2 cut(s) 104, 107
BlsI GCNGC 2 cut(s) 105, 108
Bme1390I CCNGG 2 cut(s) 89, 534
BmiI GGNNCC 2 cut(s) 520, 610
BmrFI CCNGG 2 cut(s) 89, 534
BmrI ACTGGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 324
BmuI ACTGGG 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 533, 628
Bse1I ACTGG 3 cut(s) 21, 152, 655
Bse3DI GCAATG 2 cut(s) 71, 457
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 533, 628
BseGI GGATG 3 cut(s) 147, 339, 463
BseMI GCAATG 2 cut(s) 71, 457
BseMII CTCAG 2 cut(s) 407, 460
BseNI ACTGG 3 cut(s) 21, 152, 655
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 2 cut(s) 115, 118
BsgI GTGCAG 2 cut(s) 122, 384
BshFI GGCC 3 cut(s) 87, 177, 280
BshNI GGYRCC 1 cut(s) 518
BsiSI CCGG 2 cut(s) 533, 606
BslFI GGGAC 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 3 cut(s) 87, 177, 280
Bso31I GGTCTC 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 521
Bsp143I GATC 1 cut(s) 641
BspACI CCGC 1 cut(s) 603
BspANI GGCC 3 cut(s) 87, 177, 280
BspCNI CTCAG 2 cut(s) 408, 459
BspLI GGNNCC 2 cut(s) 520, 610
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 395
BspQI GCTCTTC 1 cut(s) 27
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 413
BsrDI GCAATG 2 cut(s) 71, 457
BsrI ACTGG 3 cut(s) 21, 152, 655
BssECI CCNNGG 2 cut(s) 533, 628
BssMI GATC 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 27
BstDEI CTNAG 3 cut(s) 219, 416, 446
BstF5I GGATG 3 cut(s) 147, 339, 463
BstKTI GATC 1 cut(s) 644
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 1 cut(s) 641
BstMWI GCNNNNNNNGC 1 cut(s) 343
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 87, 532
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 115, 118
BstXI CCANNNNNNTGG 2 cut(s) 265, 470
BsuRI GGCC 3 cut(s) 87, 177, 280
BtsCI GGATG 3 cut(s) 147, 339, 463
BtsIMutI CAGTG 1 cut(s) 88
BveI ACCTGC 1 cut(s) 395
Csp6I GTAC 1 cut(s) 303
CviAII CATG 2 cut(s) 401, 433
CviJI RGCY 7 cut(s) 20, 87, 92, 177, 280, 300, 574
CviKI_1 RGCY 7 cut(s) 20, 87, 92, 177, 280, 300, 574
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 3 cut(s) 219, 416, 446
DpnI GATC 1 cut(s) 643
DpnII GATC 1 cut(s) 641
EaeI YGGCCR 2 cut(s) 85, 278
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 413
Eco32I GATATC 2 cut(s) 48, 246
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 2 cut(s) 48, 246
FaeI CATG 2 cut(s) 404, 436
FaqI GGGAC 1 cut(s) 370
FatI CATG 2 cut(s) 400, 432
FauNDI CATATG 1 cut(s) 172
Fnu4HI GCNGC 2 cut(s) 104, 107
FokI GGATG 3 cut(s) 134, 346, 450
Fsp4HI GCNGC 2 cut(s) 104, 107
FspBI CTAG 1 cut(s) 306
GluI GCNGC 2 cut(s) 104, 107
GsuI CTGGAG 1 cut(s) 422
HaeIII GGCC 3 cut(s) 87, 177, 280
HapII CCGG 2 cut(s) 533, 606
Hin1II CATG 2 cut(s) 404, 436
HinfI GANTC 3 cut(s) 368, 459, 615
HpaII CCGG 2 cut(s) 533, 606
Hpy188I TCNGA 1 cut(s) 417
Hpy188III TCNNGA 1 cut(s) 439
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4V TGCA 8 cut(s) 103, 213, 337, 365, 404, 455, 494, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 343
HpyF3I CTNAG 3 cut(s) 219, 416, 446
Hsp92II CATG 2 cut(s) 404, 436
Kzo9I GATC 1 cut(s) 641
LguI GCTCTTC 1 cut(s) 27
LmnI GCTCC 1 cut(s) 441
Lsp1109I GCAGC 2 cut(s) 115, 118
LweI GCATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 545
MalI GATC 1 cut(s) 643
MboI GATC 1 cut(s) 641
MboII GAAGA 3 cut(s) 14, 433, 521
MfeI CAATTG 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 521
MlsI TGGCCA 2 cut(s) 87, 280
MluCI AATT 2 cut(s) 161, 274
MluNI TGGCCA 2 cut(s) 87, 280
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 4 cut(s) 374, 411, 638, 690
Mox20I TGGCCA 2 cut(s) 87, 280
MscI TGGCCA 2 cut(s) 87, 280
MseI TTAA 1 cut(s) 672
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 2 cut(s) 87, 280
MspI CCGG 2 cut(s) 533, 606
MspR9I CCNGG 2 cut(s) 89, 534
MunI CAATTG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 172
NdeII GATC 1 cut(s) 641
NlaIII CATG 2 cut(s) 404, 436
NlaIV GGNNCC 2 cut(s) 520, 610
NmeAIII GCCGAG 1 cut(s) 609
NspI RCATGY 1 cut(s) 436
PciSI GCTCTTC 1 cut(s) 27
PfeI GAWTC 3 cut(s) 368, 459, 615
PkrI GCNGC 2 cut(s) 105, 108
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 520, 610
PstI CTGCAG 1 cut(s) 496
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 468
SapI GCTCTTC 1 cut(s) 27
SaqAI TTAA 1 cut(s) 672
SatI GCNGC 2 cut(s) 104, 107
Sau3AI GATC 1 cut(s) 641
ScrFI CCNGG 2 cut(s) 89, 534
SduI GDGCHC 1 cut(s) 521
SetI ASST 5 cut(s) 209, 255, 385, 409, 576
SfaNI GCATC 1 cut(s) 324
SfcI CTRYAG 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 468
SpeI ACTAGT 1 cut(s) 305
Sse9I AATT 2 cut(s) 161, 274
SsiI CCGC 1 cut(s) 603
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 2 cut(s) 87, 532
TaaI ACNGT 1 cut(s) 100
TaqI TCGA 1 cut(s) 644
TasI AATT 2 cut(s) 161, 274
TfiI GAWTC 3 cut(s) 368, 459, 615
Tru1I TTAA 1 cut(s) 672
Tru9I TTAA 1 cut(s) 672
TscAI CASTG 1 cut(s) 88
TseI GCWGC 2 cut(s) 103, 106
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 1 cut(s) 88
XceI RCATGY 1 cut(s) 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 13
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.