RLG00000002691

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005271
Physical Location & Seq
Forward (+)
38212425 .. 38213437
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002691

Sequence Viewer

Length: 720 bp
ATGAAATCCCCTTCCCCCATCTTCTGTTTGATACTCTGTGCTCTCATAAGCCTGTCAACAACTATTCTCACCGCTCAAGAAAATCCTCTTTCCTTCTACATTGCTTATCAGTGGCCAGGCTCATACTGTACAGCAGCAAAGCAGGGTTGTTGCTACCCAAAGAGTATTAGAAAACATCCCAGTTTCACAATTGGTGGCATATGGCCTTATACTTTTTCTGGGGATAGACCAACCTACTGCAAATCTAAGACCCCTTATAGTTTATCTAAGATATCAAACCTGACCAAGAGTTTGGAAAGGAATTGGCCATCACTATCCTGCCCAAGCCGTACTAGTAGCATTAGCAGTAGGAAACTATGGATGCAAGAATGGCAGAAATATGGGACTTGTGCAGAATCACTCTTTGGAGGTCAATACCAATACTTTCATGCAGGTCTCAATCTGAGGAAGAAAATAGACATGCTCCAGATACTCAGCAATGCAGGAATCCATCCAAATGGGTCATTTTATGAGATGACTGCATTTTTCAATGCCATACAAAAGGCCACCATCTATCTTCCGGGGTTTATCTGTAACAAAGACAAATCAGGCAACAGACAGCTCTACCAGATTATACTCTGTGGAAACACCGCCGGAACCAGAATCACAGACTGCCTCGGCAACCCAAGATCGAATACTGGACAATGCCCTGAAAAATTTAAGTTCCCTCCCTTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

240

Amino Acids

26.85

Weight (kDa)

9.47

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 32 - 218 4.3e-45 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 2 cut(s) 72, 630
AcoI YGGCCR 2 cut(s) 113, 305
AcsI RAATTY 1 cut(s) 695
AfaI GTAC 2 cut(s) 130, 331
AgsI TTSAA 1 cut(s) 529
AhlI ACTAGT 1 cut(s) 332
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 387, 419
AluBI AGCT 1 cut(s) 601
AluI AGCT 1 cut(s) 601
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 440
AoxI GGCC 4 cut(s) 113, 203, 305, 543
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 695
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
AsuC2I CCSGG 1 cut(s) 561
AsuHPI GGTGA 1 cut(s) 61
BalI TGGCCA 2 cut(s) 115, 307
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 1 cut(s) 146
BccI CCATC 4 cut(s) 26, 316, 498, 557
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 1 cut(s) 117
BcnI CCSGG 1 cut(s) 561
BcoDI GTCTC 1 cut(s) 440
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 117, 561
BmiI GGNNCC 1 cut(s) 637
BmrFI CCNGG 2 cut(s) 117, 561
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 351
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 561
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 560, 655
Bse1I ACTGG 2 cut(s) 180, 682
Bse3DI GCAATG 2 cut(s) 99, 484
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 560, 655
BseGI GGATG 3 cut(s) 175, 366, 490
BseMI GCAATG 2 cut(s) 99, 484
BseMII CTCAG 2 cut(s) 434, 487
BseNI ACTGG 2 cut(s) 180, 682
BseXI GCAGC 1 cut(s) 146
BsgI GTGCAG 1 cut(s) 411
BshFI GGCC 4 cut(s) 115, 205, 307, 545
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 2 cut(s) 560, 633
BslFI GGGAC 1 cut(s) 397
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 4 cut(s) 115, 205, 307, 545
Bso31I GGTCTC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 43
Bsp1407I TGTACA 1 cut(s) 128
Bsp143I GATC 1 cut(s) 668
BspACI CCGC 2 cut(s) 72, 630
BspANI GGCC 4 cut(s) 115, 205, 307, 545
BspCNI CTCAG 2 cut(s) 435, 486
BspLI GGNNCC 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 2 cut(s) 99, 484
BsrGI TGTACA 1 cut(s) 128
BsrI ACTGG 2 cut(s) 180, 682
BssECI CCNNGG 2 cut(s) 560, 655
BssMI GATC 1 cut(s) 668
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 128
BstAUI TGTACA 1 cut(s) 128
BstDEI CTNAG 4 cut(s) 246, 267, 443, 473
BstF5I GGATG 3 cut(s) 175, 366, 490
BstKTI GATC 1 cut(s) 671
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 1 cut(s) 668
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 463
BstSCI CCNGG 2 cut(s) 115, 559
BstV1I GCAGC 1 cut(s) 146
BstXI CCANNNNNNTGG 2 cut(s) 292, 497
BsuRI GGCC 4 cut(s) 115, 205, 307, 545
BtsCI GGATG 3 cut(s) 175, 366, 490
BtsIMutI CAGTG 1 cut(s) 116
BveI ACCTGC 1 cut(s) 422
Csp6I GTAC 2 cut(s) 129, 330
CviAII CATG 2 cut(s) 428, 460
CviJI RGCY 8 cut(s) 51, 115, 120, 205, 307, 327, 545, 601
CviKI_1 RGCY 8 cut(s) 51, 115, 120, 205, 307, 327, 545, 601
CviQI GTAC 2 cut(s) 129, 330
DdeI CTNAG 4 cut(s) 246, 267, 443, 473
DpnI GATC 1 cut(s) 670
DpnII GATC 1 cut(s) 668
EaeI YGGCCR 2 cut(s) 113, 305
Eco31I GGTCTC 1 cut(s) 440
Eco32I GATATC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 115
EcoRV GATATC 1 cut(s) 273
FaeI CATG 2 cut(s) 431, 463
FaqI GGGAC 1 cut(s) 397
FatI CATG 2 cut(s) 427, 459
FauNDI CATATG 1 cut(s) 200
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 3 cut(s) 162, 373, 477
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 1 cut(s) 333
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 4 cut(s) 115, 205, 307, 545
HapII CCGG 2 cut(s) 560, 633
Hin1II CATG 2 cut(s) 431, 463
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 3 cut(s) 395, 486, 642
HpaII CCGG 2 cut(s) 560, 633
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 57
Hpy188I TCNGA 1 cut(s) 444
Hpy188III TCNNGA 2 cut(s) 77, 466
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 2 cut(s) 21, 103
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 6 cut(s) 240, 364, 392, 431, 482, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 4 cut(s) 246, 267, 443, 473
Hsp92II CATG 2 cut(s) 431, 463
Kzo9I GATC 1 cut(s) 668
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 1 cut(s) 146
LweI GCATC 1 cut(s) 351
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 572
MalI GATC 1 cut(s) 670
MbiI CCGCTC 1 cut(s) 74
MboI GATC 1 cut(s) 668
MboII GAAGA 3 cut(s) 13, 460, 548
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 1 cut(s) 43
MlsI TGGCCA 2 cut(s) 115, 307
MluCI AATT 3 cut(s) 189, 301, 695
MluNI TGGCCA 2 cut(s) 115, 307
MnlI CCTC 5 cut(s) 96, 401, 438, 665, 717
Mox20I TGGCCA 2 cut(s) 115, 307
MscI TGGCCA 2 cut(s) 115, 307
MseI TTAA 2 cut(s) 699, 713
MslI CAYNNNNRTG 1 cut(s) 495
Msp20I TGGCCA 2 cut(s) 115, 307
MspI CCGG 2 cut(s) 560, 633
MspR9I CCNGG 2 cut(s) 117, 561
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 370
NciI CCSGG 1 cut(s) 561
NdeI CATATG 1 cut(s) 200
NdeII GATC 1 cut(s) 668
NlaIII CATG 2 cut(s) 431, 463
NlaIV GGNNCC 1 cut(s) 637
NmeAIII GCCGAG 1 cut(s) 636
NspI RCATGY 1 cut(s) 463
PfeI GAWTC 3 cut(s) 395, 486, 642
PkrI GCNGC 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 637
RsaI GTAC 2 cut(s) 130, 331
RsaNI GTAC 2 cut(s) 129, 330
RseI CAYNNNNRTG 1 cut(s) 495
SaqAI TTAA 2 cut(s) 699, 713
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 1 cut(s) 668
ScrFI CCNGG 2 cut(s) 117, 561
SduI GDGCHC 1 cut(s) 43
SetI ASST 5 cut(s) 236, 282, 412, 436, 603
SfaNI GCATC 1 cut(s) 351
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 3 cut(s) 189, 301, 695
SsiI CCGC 2 cut(s) 72, 630
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 115, 559
TaaI ACNGT 1 cut(s) 128
TaqI TCGA 1 cut(s) 671
TasI AATT 3 cut(s) 189, 301, 695
TatI WGTACW 1 cut(s) 128
TfiI GAWTC 3 cut(s) 395, 486, 642
Tru1I TTAA 2 cut(s) 699, 713
Tru9I TTAA 2 cut(s) 699, 713
TscAI CASTG 1 cut(s) 116
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 2 cut(s) 17, 416
TspRI CASTG 1 cut(s) 116
XapI RAATTY 1 cut(s) 695
XceI RCATGY 1 cut(s) 463
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.