MD16G1072400.v1.1

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005271
Physical Location & Seq
Reverse (-)
5070796 .. 5071750
955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1072400.v1.1.491

Sequence Viewer

Length: 741 bp
ATGTTCATCAGGCTCTTCATAATACAGTGTCTAGCAGTTGTTTCTGTTTCTCAGAGTTTTGATTTTTTCTATTTTGTACAGCAGGTGAATATTTCCGCTTGTTTTATTTTTTCATTAATTTACAGTTGTGAAGACTGGAATTTGATATATGTTGCAATGCAGTGGCCGGGATCTTACTGTGACACCGACGCAAGTTGTTGCTATCCAACGACAGGAAAACCTGCACAAGAATTCGGCATCCATGGGCTTTGGCCTAATTATAATGACGGTTCTTATCCATCCAACTGTGATTCTGACAACCCTTATGATGAATCGAAGATCTCGGACCTGATAAGCAGACTGCAAGCAGATTGGCCTACACTGGCATGCCCCAGCGGGAATGGCTCAAAGTTTTGGGCACATGAGTGGAATAAACATGGGACATGCTCCGAGCCCGTGCTTGACCAACATGGCTACTTTGAGGCATCTCTCAACCTCAAGGAAAACTTAGACATCTTCCAAATCCTTCAAAATGCTGGAATCCGAGCAGATGGGAATATTTACAGCTTGAGCAGCATAAAAGAAGCTATAAAATCAGCAATTGGGTACACTCCTGGTATAACATGTAACGAAGATGCATCTGGGAATAGCCAGCTTTATGAGGTTTATATTTGTGTGGACACATCTGGCTCAAACATTATTGAATGTCCAGTACTGCCCGGGACGAAATGCTCCTCCAAAATTGAGTTCCCTTCCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

247

Amino Acids

27.38

Weight (kDa)

4.4

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 46 - 229 3.1e-61 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 73, 229
AciI CCGC 2 cut(s) 96, 375
AclWI GGATC 1 cut(s) 178
AcoI YGGCCR 1 cut(s) 164
AcsI RAATTY 2 cut(s) 139, 230
AfaI GTAC 3 cut(s) 78, 587, 693
AfiI CCNNNNNNNGG 1 cut(s) 212
AflIII ACRYGT 1 cut(s) 602
AgsI TTSAA 2 cut(s) 509, 683
AjnI CCWGG 1 cut(s) 592
AjuI GAANNNNNNNTTGG 1 cut(s) 710
AleI CACNNNNGTG 1 cut(s) 403
AluBI AGCT 3 cut(s) 546, 566, 634
AluI AGCT 3 cut(s) 546, 566, 634
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 698
AoxI GGCC 3 cut(s) 164, 251, 353
ApeKI GCWGC 1 cut(s) 552
ApoI RAATTY 2 cut(s) 139, 230
AseI ATTAAT 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 325
AsuC2I CCSGG 3 cut(s) 168, 699, 700
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 698
AvaII GGWCC 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 400
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 564
BccI CCATC 2 cut(s) 286, 524
BciT130I CCWGG 1 cut(s) 594
BcnI CCSGG 3 cut(s) 168, 699, 700
BfaI CTAG 2 cut(s) 32, 739
BfuAI ACCTGC 2 cut(s) 73, 229
BglII AGATCT 1 cut(s) 318
BisI GCNGC 1 cut(s) 553
BlsI GCNGC 1 cut(s) 554
BmcAI AGTACT 1 cut(s) 693
Bme1390I CCNGG 4 cut(s) 168, 594, 699, 700
Bme18I GGWCC 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 698
BmgT120I GGNCC 1 cut(s) 325
BmrFI CCNGG 4 cut(s) 168, 594, 699, 700
BmsI GCATC 4 cut(s) 246, 473, 604, 626
BpiI GAAGAC 1 cut(s) 138
BpuEI CTTGAG 2 cut(s) 461, 568
BpuMI CCSGG 3 cut(s) 168, 699, 700
BsaJI CCNNGG 2 cut(s) 241, 698
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse1I ACTGG 3 cut(s) 140, 366, 689
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 594
BseDI CCNNGG 2 cut(s) 241, 698
BseGI GGATG 2 cut(s) 237, 278
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 3 cut(s) 140, 366, 689
BseRI GAGGAG 1 cut(s) 703
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 1 cut(s) 564
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 3 cut(s) 166, 253, 355
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 2 cut(s) 167, 699
BslFI GGGAC 2 cut(s) 433, 715
BslI CCNNNNNNNGG 1 cut(s) 212
BsmFI GGGAC 2 cut(s) 433, 715
BsnI GGCC 3 cut(s) 166, 253, 355
BsoBI CYCGRG 1 cut(s) 698
Bsp1286I GDGCHC 2 cut(s) 400, 435
Bsp1407I TGTACA 1 cut(s) 76
Bsp143I GATC 2 cut(s) 170, 318
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 2 cut(s) 96, 375
BspANI GGCC 3 cut(s) 166, 253, 355
BspCNI CTCAG 1 cut(s) 64
BspMI ACCTGC 2 cut(s) 73, 229
BspPI GGATC 1 cut(s) 178
BspQI GCTCTTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 162
BsrGI TGTACA 1 cut(s) 76
BsrI ACTGG 3 cut(s) 140, 366, 689
BssECI CCNNGG 2 cut(s) 241, 698
BssMI GATC 2 cut(s) 170, 318
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 5 cut(s) 27, 125, 179, 269, 287
Bst6I CTCTTC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 76
BstC8I GCNNGC 3 cut(s) 345, 367, 632
BstDEI CTNAG 2 cut(s) 51, 487
BstDSI CCRYGG 1 cut(s) 241
BstF5I GGATG 2 cut(s) 237, 278
BstKTI GATC 2 cut(s) 173, 321
BstMBI GATC 2 cut(s) 170, 318
BstMWI GCNNNNNNNGC 2 cut(s) 381, 552
BstNI CCWGG 1 cut(s) 594
BstNSI RCATGY 3 cut(s) 369, 426, 606
BstSCI CCNGG 4 cut(s) 166, 592, 697, 698
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 564
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 2 cut(s) 170, 318
BstYI RGATCY 2 cut(s) 170, 318
BsuRI GGCC 3 cut(s) 166, 253, 355
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 237, 278
BtsI GCAGTG 1 cut(s) 167
BtsIMutI CAGTG 3 cut(s) 32, 167, 359
BveI ACCTGC 2 cut(s) 73, 229
Cac8I GCNNGC 3 cut(s) 345, 367, 632
Cfr13I GGNCC 1 cut(s) 325
Cfr9I CCCGGG 1 cut(s) 698
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 77, 586, 692
CviAII CATG 7 cut(s) 242, 366, 401, 416, 423, 449, 603
CviQI GTAC 3 cut(s) 77, 586, 692
DdeI CTNAG 2 cut(s) 51, 487
DpnI GATC 2 cut(s) 172, 320
DpnII GATC 2 cut(s) 170, 318
EaeI YGGCCR 1 cut(s) 164
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 592
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 7 cut(s) 245, 369, 404, 419, 426, 452, 606
FalI AAGNNNNNCTT 2 cut(s) 470, 502
FaqI GGGAC 2 cut(s) 433, 715
FatI CATG 7 cut(s) 241, 365, 400, 415, 422, 448, 602
FauI CCCGC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 553
FokI GGATG 2 cut(s) 224, 265
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 1 cut(s) 553
FspBI CTAG 2 cut(s) 32, 739
GluI GCNGC 1 cut(s) 553
GsaI CCCAGC 1 cut(s) 375
HaeIII GGCC 3 cut(s) 166, 253, 355
HapII CCGG 2 cut(s) 167, 699
HgaI GACGC 1 cut(s) 197
Hin1II CATG 7 cut(s) 245, 369, 404, 419, 426, 452, 606
HinfI GANTC 3 cut(s) 290, 311, 519
HpaII CCGG 2 cut(s) 167, 699
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 588, 658
Hpy188I TCNGA 5 cut(s) 54, 295, 325, 430, 524
Hpy8I GTNNAC 2 cut(s) 588, 658
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 515, 741
HpyCH4III ACNGT 5 cut(s) 27, 125, 179, 269, 287
HpyCH4V TGCA 5 cut(s) 155, 160, 224, 343, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 552
HpyF3I CTNAG 2 cut(s) 51, 487
Hsp92II CATG 7 cut(s) 245, 369, 404, 419, 426, 452, 606
Kzo9I GATC 2 cut(s) 170, 318
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 2 cut(s) 431, 716
Lsp1109I GCAGC 1 cut(s) 564
LweI GCATC 4 cut(s) 246, 473, 604, 626
MaeI CTAG 2 cut(s) 32, 739
MaeIII GTNAC 2 cut(s) 179, 605
MalI GATC 2 cut(s) 172, 320
MboI GATC 2 cut(s) 170, 318
MboII GAAGA 5 cut(s) 7, 143, 328, 487, 623
MfeI CAATTG 1 cut(s) 579
MflI RGATCY 2 cut(s) 170, 318
MhlI GDGCHC 2 cut(s) 400, 435
MluCI AATT 6 cut(s) 117, 139, 230, 256, 579, 720
MmeI TCCRAC 2 cut(s) 230, 306
MnlI CCTC 4 cut(s) 454, 485, 634, 724
Mph1103I ATGCAT 1 cut(s) 619
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 364, 403
MspA1I CMGCKG 1 cut(s) 375
MspI CCGG 2 cut(s) 167, 699
MspR9I CCNGG 4 cut(s) 168, 594, 699, 700
MunI CAATTG 1 cut(s) 579
MvaI CCWGG 1 cut(s) 594
MwoI GCNNNNNNNGC 2 cut(s) 381, 552
NciI CCSGG 3 cut(s) 168, 699, 700
NcoI CCATGG 1 cut(s) 241
NdeII GATC 2 cut(s) 170, 318
NlaIII CATG 7 cut(s) 245, 369, 404, 419, 426, 452, 606
NmuCI GTSAC 1 cut(s) 179
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 3 cut(s) 369, 426, 606
OliI CACNNNNGTG 1 cut(s) 403
PaeI GCATGC 1 cut(s) 369
PaqCI CACCTGC 1 cut(s) 73
PciI ACATGT 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 20
PfeI GAWTC 3 cut(s) 290, 311, 519
PkrI GCNGC 1 cut(s) 554
PscI ACATGT 1 cut(s) 602
PshBI ATTAAT 1 cut(s) 116
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 592
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 1 cut(s) 592
PspPI GGNCC 1 cut(s) 325
PsuI RGATCY 2 cut(s) 170, 318
RsaI GTAC 3 cut(s) 78, 587, 693
RsaNI GTAC 3 cut(s) 77, 586, 692
RseI CAYNNNNRTG 2 cut(s) 364, 403
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 1 cut(s) 553
Sau3AI GATC 2 cut(s) 170, 318
Sau96I GGNCC 1 cut(s) 325
ScaI AGTACT 1 cut(s) 693
ScrFI CCNGG 4 cut(s) 168, 594, 699, 700
SduI GDGCHC 2 cut(s) 400, 435
SetI ASST 8 cut(s) 87, 223, 330, 477, 548, 568, 636, 645
SfaNI GCATC 4 cut(s) 246, 473, 604, 626
SinI GGWCC 1 cut(s) 325
SmaI CCCGGG 1 cut(s) 700
SmiMI CAYNNNNRTG 2 cut(s) 364, 403
SmlI CTYRAG 2 cut(s) 476, 547
SmoI CTYRAG 2 cut(s) 476, 547
SphI GCATGC 1 cut(s) 369
Sse9I AATT 6 cut(s) 117, 139, 230, 256, 579, 720
SsiI CCGC 2 cut(s) 96, 375
SspI AATATT 2 cut(s) 91, 538
SspMI CTAG 2 cut(s) 32, 739
StyD4I CCNGG 4 cut(s) 166, 592, 697, 698
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 5 cut(s) 27, 125, 179, 269, 287
TaqI TCGA 1 cut(s) 314
TasI AATT 6 cut(s) 117, 139, 230, 256, 579, 720
TatI WGTACW 2 cut(s) 76, 691
TfiI GAWTC 3 cut(s) 290, 311, 519
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 3 cut(s) 32, 167, 366
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 1 cut(s) 552
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 3 cut(s) 7, 102, 324
TspMI CCCGGG 1 cut(s) 698
TspRI CASTG 3 cut(s) 32, 167, 366
VpaK11BI GGWCC 1 cut(s) 325
VspI ATTAAT 1 cut(s) 116
XapI RAATTY 2 cut(s) 139, 230
XceI RCATGY 3 cut(s) 369, 426, 606
XmaI CCCGGG 1 cut(s) 698
XspI CTAG 2 cut(s) 32, 739
ZrmI AGTACT 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.