Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005271

Physical Location & Seq

Genomic Coordinates Reverse (-)

5066570 .. 5068327

Gene Structure 1758 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1072300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 681 bp

ATGAAGTCCAACCCCTCAACCTTGGTCAAGCTTTTACTGATAGGATGTCTTTCAGTTGTTTGCATTGCAGAAGATTTTGATTTCTTCTACTTTGTCCAGCAGTGGCCCGGATCATATTGTGACACAAAGACAAGTTGCTGCTATCCAACAACAGGGAAGCCTGCAGCTGATTTCGGCATTCATGGGCTTTGGCCGAATTACAACGACGGCTCATATCCCTCCAACTGCGATCCTAGCAACCCCTTTGATCAATCTGAGATTTCAGATCTAAGAAGCAGCATGCAAAAAGAATGGCCAACACTAGCTTGCCCAAGCAGCAGCGGCATAGCGTTCTGGACACATGAATGGGAGAAACATGGCACCTGCTCAGAGTCTGTCATCGACCAACACGGTTACTTTGAAGCTGCTCTTAACCTCAAAAAGAAAATAAACCTCCTCCAAGCCCTCGAAAGTGCAGGTATACAGCCAAATGGGGACTCATACACCCTACAGAACATCACGGGGGCAGTGAAAGATGCAACTGGATTCACTCCATTTATTGAATGCAATGTGGATGAATCTGGTAACAGCCAACTTTATCAGGTTTACTTGTGTGTGGACACTTCTGGTTCTGACCTCATTGAGTGTCCTGTTTTTCCCCATGGACAGTGTGGTGCACAGACTGAATTCCCAACTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004540 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0005886 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009267 GO:0009605 GO:0009611 GO:0009718 GO:0009812 GO:0009813 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016070 GO:0016787 GO:0016788 GO:0030312 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0034641 GO:0042440 GO:0042594 GO:0043170 GO:0044237 GO:0044238 GO:0044464 GO:0046148 GO:0046283 GO:0046483 GO:0050896 GO:0051716 GO:0071496 GO:0071704 GO:0071944 GO:0090304 GO:0090305 GO:0090501 GO:0140098 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ribonuclease_T2

Protein Analysis

227

Amino Acids

24.87

Weight (kDa)

4.45

Isoelectric Point (pI)

44.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ribonuclease_T2	PF00445	26 - 209	5.8e-65	Ribonuclease T2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005271)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G02990
fragaria_vesca	FvH4_4g31291 FvH4_5g24800
malus_domestica	MD13G1071600.v1.1 MD16G1072300.v1.1 MD16G1072400.v1.1
prunus_persica	Prupe.1G275300_v2.0.a1
pyrus_communis	pycom13g06420 pycom16g06270
rosa_chinensis	RchiOBHm_Chr4g0439741 RchiOBHm_Chr7g0215091 RchiOBHm_Chr7g0215101
rosa_laevigata	RLG00000002691 RLG00000006240
rosa_multiflora	Rmu_sc0001239.1_g000001 Rmu_sc0010226.1_g000008 Rmu_ssc0000029.1_g000010
rosa_roxburghii	Rroxscaffold_5G00380680
rosa_rugosa	Rorug04G0321200 Rorug07G0150100
rosa_samantha	Rh4AG372200 Rh4BG384300 Rh4CG399000 Rh4DG378700 Rh7AG287100 Rh7BG278700 Rh7CG306800
rosa_wichuraiana	Rw4G031780 Rw7G024480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	371
Acc36I	ACCTGC	2 cut(s)	371, 446
AccB1I	GGYRCC	1 cut(s)	359
AccI	GTMKAC	1 cut(s)	460
AciI	CCGC	1 cut(s)	321
AclWI	GGATC	2 cut(s)	118, 224
AcoI	YGGCCR	2 cut(s)	191, 293
AcsI	RAATTY	1 cut(s)	665
AfiI	CCNNNNNNNGG	1 cut(s)	152
AgsI	TTSAA	2 cut(s)	401, 542
AluBI	AGCT	4 cut(s)	31, 167, 305, 404
AluI	AGCT	4 cut(s)	31, 167, 305, 404
Alw21I	GWGCWC	1 cut(s)	658
Alw44I	GTGCAC	1 cut(s)	654
AlwI	GGATC	2 cut(s)	118, 224
AlwNI	CAGNNNCTG	1 cut(s)	374
AoxI	GGCC	3 cut(s)	104, 191, 293
ApaLI	GTGCAC	1 cut(s)	654
ApeKI	GCWGC	6 cut(s)	138, 164, 276, 315, 318, 404
ApoI	RAATTY	1 cut(s)	665
AspS9I	GGNCC	1 cut(s)	105
AsuC2I	CCSGG	1 cut(s)	108
BaeGI	GKGCMC	1 cut(s)	658
BalI	TGGCCA	1 cut(s)	295
BanI	GGYRCC	1 cut(s)	359
Bbv12I	GWGCWC	1 cut(s)	658
BbvI	GCAGC	6 cut(s)	125, 176, 288, 327, 330, 391
BceAI	ACGGC	1 cut(s)	223
BclI	TGATCA	1 cut(s)	247
BcnI	CCSGG	1 cut(s)	108
BfaI	CTAG	2 cut(s)	234, 302
BfmI	CTRYAG	2 cut(s)	162, 488
BfuAI	ACCTGC	2 cut(s)	371, 446
BglII	AGATCT	1 cut(s)	265
BisI	GCNGC	7 cut(s)	139, 165, 277, 316, 319, 322, 405
BlsI	GCNGC	7 cut(s)	140, 166, 278, 317, 320, 323, 406
Bme1390I	CCNGG	1 cut(s)	108
BmgT120I	GGNCC	1 cut(s)	105
BmiI	GGNNCC	1 cut(s)	361
BmrFI	CCNGG	1 cut(s)	108
BmsI	GCATC	1 cut(s)	505
BpuMI	CCSGG	1 cut(s)	108
BsaJI	CCNNGG	2 cut(s)	21, 640
Bsc4I	CCNNNNNNNGG	1 cut(s)	152
Bse1I	ACTGG	1 cut(s)	526
Bse3DI	GCAATG	2 cut(s)	63, 553
BseDI	CCNNGG	2 cut(s)	21, 640
BseGI	GGATG	2 cut(s)	50, 559
BseLI	CCNNNNNNNGG	1 cut(s)	152
BseMI	GCAATG	2 cut(s)	63, 553
BseMII	CTCAG	2 cut(s)	246, 381
BseNI	ACTGG	1 cut(s)	526
BseRI	GAGGAG	1 cut(s)	425
BseSI	GKGCMC	1 cut(s)	658
BseXI	GCAGC	6 cut(s)	125, 176, 288, 327, 330, 391
BsgI	GTGCAG	1 cut(s)	474
BshFI	GGCC	3 cut(s)	106, 193, 295
BshNI	GGYRCC	1 cut(s)	359
BsiHKAI	GWGCWC	1 cut(s)	658
BsiSI	CCGG	1 cut(s)	108
BslFI	GGGAC	1 cut(s)	488
BslI	CCNNNNNNNGG	1 cut(s)	152
BsmFI	GGGAC	1 cut(s)	488
BsmI	GAATGC	2 cut(s)	177, 548
BsnI	GGCC	3 cut(s)	106, 193, 295
Bsp1286I	GDGCHC	1 cut(s)	658
Bsp143I	GATC	4 cut(s)	110, 229, 247, 265
Bsp19I	CCATGG	1 cut(s)	640
BspACI	CCGC	1 cut(s)	321
BspANI	GGCC	3 cut(s)	106, 193, 295
BspCNI	CTCAG	2 cut(s)	247, 380
BspLI	GGNNCC	1 cut(s)	361
BspMAI	CTGCAG	1 cut(s)	166
BspMI	ACCTGC	2 cut(s)	371, 446
BspPI	GGATC	2 cut(s)	118, 224
BspT107I	GGYRCC	1 cut(s)	359
BsrDI	GCAATG	2 cut(s)	63, 553
BsrI	ACTGG	1 cut(s)	526
BssECI	CCNNGG	2 cut(s)	21, 640
BssMI	GATC	4 cut(s)	110, 229, 247, 265
BssNAI	GTATAC	1 cut(s)	461
BssT1I	CCWWGG	2 cut(s)	21, 640
Bst1107I	GTATAC	1 cut(s)	461
Bst4CI	ACNGT	2 cut(s)	392, 648
BstC8I	GCNNGC	3 cut(s)	162, 281, 307
BstDEI	CTNAG	3 cut(s)	255, 269, 367
BstDSI	CCRYGG	1 cut(s)	640
BstF5I	GGATG	2 cut(s)	50, 559
BstKTI	GATC	4 cut(s)	113, 232, 250, 268
BstMBI	GATC	4 cut(s)	110, 229, 247, 265
BstMWI	GCNNNNNNNGC	3 cut(s)	234, 315, 321
BstNSI	RCATGY	1 cut(s)	283
BstSCI	CCNGG	1 cut(s)	106
BstSFI	CTRYAG	2 cut(s)	162, 488
BstSLI	GKGCMC	1 cut(s)	658
BstV1I	GCAGC	6 cut(s)	125, 176, 288, 327, 330, 391
BstX2I	RGATCY	1 cut(s)	265
BstYI	RGATCY	1 cut(s)	265
BstZ17I	GTATAC	1 cut(s)	461
BsuRI	GGCC	3 cut(s)	106, 193, 295
BtgI	CCRYGG	1 cut(s)	640
BtsCI	GGATG	2 cut(s)	50, 559
BtsI	GCAGTG	2 cut(s)	107, 513
BtsIMutI	CAGTG	3 cut(s)	107, 513, 653
BveI	ACCTGC	2 cut(s)	371, 446
Cac8I	GCNNGC	3 cut(s)	162, 281, 307
CaiI	CAGNNNCTG	1 cut(s)	374
Cfr13I	GGNCC	1 cut(s)	105
CviAII	CATG	5 cut(s)	182, 280, 341, 356, 641
DdeI	CTNAG	3 cut(s)	255, 269, 367
DpnI	GATC	4 cut(s)	112, 231, 249, 267
DpnII	GATC	4 cut(s)	110, 229, 247, 265
EaeI	YGGCCR	2 cut(s)	191, 293
Eco130I	CCWWGG	2 cut(s)	21, 640
EcoRI	GAATTC	1 cut(s)	665
EcoT14I	CCWWGG	2 cut(s)	21, 640
ErhI	CCWWGG	2 cut(s)	21, 640
FaeI	CATG	5 cut(s)	185, 283, 344, 359, 644
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	1 cut(s)	488
FatI	CATG	5 cut(s)	181, 279, 340, 355, 640
FbaI	TGATCA	1 cut(s)	247
FblI	GTMKAC	1 cut(s)	460
Fnu4HI	GCNGC	7 cut(s)	139, 165, 277, 316, 319, 322, 405
FokI	GGATG	2 cut(s)	57, 566
Fsp4HI	GCNGC	7 cut(s)	139, 165, 277, 316, 319, 322, 405
FspBI	CTAG	2 cut(s)	234, 302
GluI	GCNGC	7 cut(s)	139, 165, 277, 316, 319, 322, 405
HaeIII	GGCC	3 cut(s)	106, 193, 295
HapII	CCGG	1 cut(s)	108
Hin1II	CATG	5 cut(s)	185, 283, 344, 359, 644
HindIII	AAGCTT	1 cut(s)	29
HinfI	GANTC	4 cut(s)	371, 476, 525, 557
HpaII	CCGG	1 cut(s)	108
Hpy166II	GTNNAC	4 cut(s)	461, 586, 598, 656
Hpy188I	TCNGA	4 cut(s)	256, 265, 370, 613
Hpy188III	TCNNGA	1 cut(s)	334
Hpy8I	GTNNAC	4 cut(s)	461, 586, 598, 656
Hpy99I	CGWCG	1 cut(s)	209
HpyCH4III	ACNGT	2 cut(s)	392, 648
HpyCH4V	TGCA	8 cut(s)	63, 68, 164, 283, 455, 518, 546, 656
HpyF10VI	GCNNNNNNNGC	3 cut(s)	234, 315, 321
HpyF3I	CTNAG	3 cut(s)	255, 269, 367
Hsp92II	CATG	5 cut(s)	185, 283, 344, 359, 644
Ksp22I	TGATCA	1 cut(s)	247
Kzo9I	GATC	4 cut(s)	110, 229, 247, 265
Lsp1109I	GCAGC	6 cut(s)	125, 176, 288, 327, 330, 391
LweI	GCATC	1 cut(s)	505
MaeI	CTAG	2 cut(s)	234, 302
MaeIII	GTNAC	3 cut(s)	119, 392, 563
MalI	GATC	4 cut(s)	112, 231, 249, 267
MboI	GATC	4 cut(s)	110, 229, 247, 265
MboII	GAAGA	2 cut(s)	76, 83
MflI	RGATCY	1 cut(s)	265
MhlI	GDGCHC	1 cut(s)	658
MlsI	TGGCCA	1 cut(s)	295
MluCI	AATT	2 cut(s)	196, 665
MluNI	TGGCCA	1 cut(s)	295
MlyI	GAGTC	2 cut(s)	380, 470
MmeI	TCCRAC	3 cut(s)	33, 170, 246
MnlI	CCTC	7 cut(s)	25, 229, 425, 443, 446, 455, 626
Mox20I	TGGCCA	1 cut(s)	295
MscI	TGGCCA	1 cut(s)	295
MseI	TTAA	1 cut(s)	411
MslI	CAYNNNNRTG	1 cut(s)	343
Msp20I	TGGCCA	1 cut(s)	295
MspA1I	CMGCKG	2 cut(s)	167, 321
MspI	CCGG	1 cut(s)	108
MspR9I	CCNGG	1 cut(s)	108
Mva1269I	GAATGC	2 cut(s)	177, 548
MwoI	GCNNNNNNNGC	3 cut(s)	234, 315, 321
NciI	CCSGG	1 cut(s)	108
NcoI	CCATGG	1 cut(s)	640
NdeII	GATC	4 cut(s)	110, 229, 247, 265
NlaIII	CATG	5 cut(s)	185, 283, 344, 359, 644
NlaIV	GGNNCC	1 cut(s)	361
NmuCI	GTSAC	1 cut(s)	119
NspI	RCATGY	1 cut(s)	283
PaeI	GCATGC	1 cut(s)	283
PaqCI	CACCTGC	1 cut(s)	371
PctI	GAATGC	2 cut(s)	177, 548
PfeI	GAWTC	2 cut(s)	525, 557
PkrI	GCNGC	7 cut(s)	140, 166, 278, 317, 320, 323, 406
PleI	GAGTC	2 cut(s)	379, 470
PpsI	GAGTC	2 cut(s)	379, 470
PspN4I	GGNNCC	1 cut(s)	361
PspPI	GGNCC	1 cut(s)	105
PstI	CTGCAG	1 cut(s)	166
PstNI	CAGNNNCTG	1 cut(s)	374
PsuI	RGATCY	1 cut(s)	265
PvuII	CAGCTG	1 cut(s)	167
RseI	CAYNNNNRTG	1 cut(s)	343
SaqAI	TTAA	1 cut(s)	411
SatI	GCNGC	7 cut(s)	139, 165, 277, 316, 319, 322, 405
Sau3AI	GATC	4 cut(s)	110, 229, 247, 265
Sau96I	GGNCC	1 cut(s)	105
SchI	GAGTC	2 cut(s)	380, 470
ScrFI	CCNGG	1 cut(s)	108
SduI	GDGCHC	1 cut(s)	658
SfaNI	GCATC	1 cut(s)	505
SfcI	CTRYAG	2 cut(s)	162, 488
SmiMI	CAYNNNNRTG	1 cut(s)	343
SphI	GCATGC	1 cut(s)	283
Sse9I	AATT	2 cut(s)	196, 665
SsiI	CCGC	1 cut(s)	321
SspMI	CTAG	2 cut(s)	234, 302
StyD4I	CCNGG	1 cut(s)	106
StyI	CCWWGG	2 cut(s)	21, 640
TaaI	ACNGT	2 cut(s)	392, 648
TaqI	TCGA	2 cut(s)	381, 447
TasI	AATT	2 cut(s)	196, 665
TauI	GCSGC	1 cut(s)	324
TfiI	GAWTC	2 cut(s)	525, 557
Tru1I	TTAA	1 cut(s)	411
Tru9I	TTAA	1 cut(s)	411
TscAI	CASTG	3 cut(s)	107, 513, 653
TseFI	GTSAC	1 cut(s)	119
TseI	GCWGC	6 cut(s)	138, 164, 276, 315, 318, 404
Tsp45I	GTSAC	1 cut(s)	119
TspDTI	ATGAA	4 cut(s)	17, 170, 357, 570
TspRI	CASTG	3 cut(s)	107, 513, 653
VneI	GTGCAC	1 cut(s)	654
XapI	RAATTY	1 cut(s)	665
XceI	RCATGY	1 cut(s)	283
XcmI	CCANNNNNNNNNTGG	1 cut(s)	647
XmiI	GTMKAC	1 cut(s)	460
XspI	CTAG	2 cut(s)	234, 302

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.