Rw7G024480

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005271
Physical Location & Seq
Reverse (-)
30161373 .. 30162273
901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G024480.1

Sequence Viewer

Length: 468 bp
ATGAAATCCCATTCCCCCATCTTCTGTTTGGTACTCTCTGCTCTCATAAGCCTGTCAACAACTATTCTAACCGCTCAAGAAAATCCTGTTTCCTTCTACATTGCTGATCAGTGGCCAGGCTCATACTGTGCAGCAACAAATCAGGGTTGTTGCTACCCAAAGAGTATTAGAAAACATCCCAGTTTCACAATTGGTGGCATATGCAGTAAGAAACTATGGATGCAAGAATGGCAGAAATATGGGACTTGTGCAGAATCACTCTTTGGAGGTCAATACCAATACTTTCATGCAGGTCTCAATCTCAGGAAGAAAATAGACATGCTCCAGATACTCAGTAATGCAGGAATCCATCCAAATGGGTCATTTTATGAGATGACTGCAGTTTTCAATGCCATACAAAAGGCCACCATCTATCTTCCGGGGTTTATCTGTAACAAAGACAAATCAGGCAACAAACAGCTCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

155

Amino Acids

17.34

Weight (kDa)

9.07

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 70 - 155 3.5e-20 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 74
AciI CCGC 1 cut(s) 72
AcoI YGGCCR 1 cut(s) 113
AfaI GTAC 1 cut(s) 33
AgsI TTSAA 1 cut(s) 388
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 1 cut(s) 460
AluI AGCT 1 cut(s) 460
Alw26I GTCTC 1 cut(s) 299
AoxI GGCC 2 cut(s) 113, 402
ApeKI GCWGC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 420
BalI TGGCCA 1 cut(s) 115
BbvI GCAGC 1 cut(s) 143
BccI CCATC 3 cut(s) 26, 357, 416
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 1 cut(s) 420
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 1 cut(s) 466
BfmI CTRYAG 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 2 cut(s) 117, 420
BmrFI CCNGG 2 cut(s) 117, 420
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 3 cut(s) 175, 225, 349
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 2 cut(s) 316, 346
BseNI ACTGG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 143
BsgI GTGCAG 2 cut(s) 150, 270
BshFI GGCC 2 cut(s) 115, 404
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 2 cut(s) 115, 404
Bso31I GGTCTC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 106
BspACI CCGC 1 cut(s) 72
BspANI GGCC 2 cut(s) 115, 404
BspCNI CTCAG 2 cut(s) 315, 345
BspMAI CTGCAG 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 1 cut(s) 106
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 128
BstDEI CTNAG 2 cut(s) 302, 332
BstF5I GGATG 3 cut(s) 175, 225, 349
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 115, 418
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 143
BstXI CCANNNNNNTGG 1 cut(s) 356
BsuRI GGCC 2 cut(s) 115, 404
BtsCI GGATG 3 cut(s) 175, 225, 349
BtsIMutI CAGTG 1 cut(s) 116
BveI ACCTGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 32
CviAII CATG 2 cut(s) 287, 319
CviJI RGCY 5 cut(s) 51, 115, 120, 404, 460
CviKI_1 RGCY 5 cut(s) 51, 115, 120, 404, 460
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 2 cut(s) 302, 332
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 115
FaeI CATG 2 cut(s) 290, 322
FaqI GGGAC 1 cut(s) 256
FatI CATG 2 cut(s) 286, 318
FauNDI CATATG 1 cut(s) 200
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 3 cut(s) 162, 232, 336
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 466
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 2 cut(s) 115, 404
HapII CCGG 1 cut(s) 419
Hin1II CATG 2 cut(s) 290, 322
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 2 cut(s) 254, 345
HpaII CCGG 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 57
Hpy188III TCNNGA 3 cut(s) 77, 304, 325
Hpy8I GTNNAC 1 cut(s) 57
HpyAV CCTTC 1 cut(s) 103
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 7 cut(s) 131, 204, 223, 251, 290, 341, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 2 cut(s) 302, 332
Hsp92II CATG 2 cut(s) 290, 322
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 1 cut(s) 143
LweI GCATC 1 cut(s) 210
MaeI CTAG 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 431
MalI GATC 1 cut(s) 108
MbiI CCGCTC 1 cut(s) 74
MboI GATC 1 cut(s) 106
MboII GAAGA 3 cut(s) 13, 319, 407
MfeI CAATTG 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 115
MluCI AATT 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 115
MnlI CCTC 1 cut(s) 260
Mox20I TGGCCA 1 cut(s) 115
MscI TGGCCA 1 cut(s) 115
MslI CAYNNNNRTG 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 115
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 117, 420
MunI CAATTG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 1 cut(s) 229
NciI CCSGG 1 cut(s) 420
NdeI CATATG 1 cut(s) 200
NdeII GATC 1 cut(s) 106
NlaIII CATG 2 cut(s) 290, 322
NspI RCATGY 1 cut(s) 322
PfeI GAWTC 2 cut(s) 254, 345
PkrI GCNGC 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PstI CTGCAG 1 cut(s) 382
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 354
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 117, 420
SetI ASST 3 cut(s) 271, 295, 462
SfaNI GCATC 1 cut(s) 210
SfcI CTRYAG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 1 cut(s) 189
SsiI CCGC 1 cut(s) 72
SspMI CTAG 1 cut(s) 466
StyD4I CCNGG 2 cut(s) 115, 418
TaaI ACNGT 1 cut(s) 128
TasI AATT 1 cut(s) 189
TfiI GAWTC 2 cut(s) 254, 345
TscAI CASTG 1 cut(s) 116
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 2 cut(s) 17, 275
TspRI CASTG 1 cut(s) 116
XceI RCATGY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.