Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Forward (+)

28053794 .. 28067810

Gene Structure 14017 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g38000.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6807 bp

ATGTCAGAAGCTCAAAGGAGATTGATTTCAACTGTTAGTATTCCTCGTACTAATGGGTACGTAAATGGGGGACTATCAATCAGGTCCCCTGCTGCGGCACCTGCAGTTGAGGAGTTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATAGCAAACAATGGAATGGCAGCAGTCAAGTTTATACGTAGTGTGAGGACATGGGCTTATGAAACATTTGGCACAGAAAAGGCTGTTTTGTTGGTGGCCATGGCCACACCAGAAGACATGAGAATCAATGCAGAACACATTAGAATTGCTGATCAGTTTGTGGAAGTTCCTGGTGGAACTAACAATAATAACTATGCCAATGTGCAGCTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTGTGGCCTGGTTGGGGCCATGCATCTGAGATCCCTGAGCTTCCAGATGCATTGACTGCTAAGGGCATAATATTTCTTGGGCCACCAGCTGTCTCTATGGCAGCATTAGGAGATAAAATCGGTTCATCATTAATTGCTCAATCTGCTGAAGTACCTACCCTTCCATGGAGCGGATCACATGTGAAAATTCCTTCAGAGAGCTGCCTGGTTACAATCCCAGATGAAATATATAGGGAAGCATGCGTTTACACAACAGAGGAAGCAATTGCAAGTTGTCAGGTTGTTGGTTACCCGGCCATGATTAAGGCATCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAAGTTAGGGCATTGTTCAAGCAAGTCCAGGGTGAAGTTCCTGGATCTCCCATATTCATAATGAAAGTCGCGTCCCAGAGCCGACATTTAGAAGTCCAGTTACTATGTGATCAGCATGGAAATGTTGCAGCTTTGCACAGTCGTGATTGCAGTGTTCAAAGGCGGCACCAGAAGATTATTGAGGAGGGTCCAATAACTGTAGCTCCACCAGAGACAATTAAAAAGTTGGAGCAGTCAGCTCGAAGGTTGGCTAAATGTGTTAATTATGTTGGAGCAGCAACTGTTGAGTATTTGTACAGTATGGACACTGGCGAGTACTATTTCTTAGAGTTGAACCCTCGGTTGCAGGTGGAGCACCCAGTCACTGAGTGGATAGCAGAAATAAATCTGCCAGCAGCCCAAGTTGCAGTTGGGATGGGCATTCCTCTCTGGCAAATTCCTGAGATAAGGCGGTTCTATGGAATGGAACATGGTAGTGGATATGATGCGTGGAGAAAGACGTCGGTTGTTGCCACCCCGTTTGACTTTGACAAGGCAGAATCTACAAGGCCAAAGGGCCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAACCTACTAGTGGGAAAGTTCAGGAGCTGAGTTTCAAAAGCAAGCCAAATGTTTGGGCATACTTTTCTGTTAAGTCTGGAGGGGGGATTCATGAGTTCTCAGATTCTCAGTTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGAACAAATGTGGATTATTCAATTGATCTTTTACATGCTTCAGATTACAGAGAAAATAAAATCCACACAGGTTGGTTGGATAGTAGAATTGCTATGCGAGTTAGAACTGAAAGGCCTCCTTGGTATCTGTCTGTAATTGGAGGGACTCTTTCTAAAGCATCTGCTAGTAGTGCGGCGATGGTTTCAGATTATATTGGCTATCTTGAGAAGGGGCAAATCCCACCTAAGCATATATCATTTGTCCATTCACAAGTGTCTTTGAACATCGAGGGGAGCAAATACACGATTGATATGGTGAGAGGAGGCCCTGGGAACTATAGATTGCGGATGAATGACTCAGAGGTTGAAGCAGAGATACATACTTTACGCGATGGAGGATTACTGATGCAGTTGGATGGAAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGCCTTCTTATTGATGGACGGACATGCTTGCTTCAGAACGATCATGATCCATCCAAGTTAATAGCAGAGACACCATGCAAGCTGTTGAGGTTCTTGGTTGCTGATGATAGTCATGTCGATGCTGATACACCATATGCGGAGGTTGAGGTTATGAAGATGTGTATGCCTCTACTTTCACCTGCTTCAGGAGTTATCCACTTTCAATTGTCTGAAGGCCAGGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGACCCTTCGGCTGTAAGAAAGGCAGAACCTTTCCATGGAAGCTTCCCTGTCCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTTCATCAGAGATGTGCTGCAAGCTTAAACGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCAATTGCCTAGATAGTCCCGAACTCCCTTTCCTTCAATGGCAAGAATGCTTGGCTGTTTTAGCAACCCGCCTACCCAAGAATCTGAAAAATGAACTGGAATCAAAATGTAAGGATTTTGAGCTGATTTCTAGCTCTCAGAATGTTGATTTCCCTGCCAAATTGTTGCGTAGCGTTCTCGAGGCTCATCTGTTCTCCTCTCCTGATAAAGAAAAAGGAGCCCAAGAAAGGCTTGTTGAACCTTTAATGAGCCTTGTGAAGTCTTATGAAGGTGGAAGAGAAAGTCATGCCCGAGTTATTGTTCAATCCCTTTTTGAAGAGTATCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTAATTGAGCGCCTTCGACTTCAATATAAGAAGGATCTATTGAAGGTTGTGGAAATTGTGCTCTCTCATCAGGGTGTAAAGAATAAAAATAAGCTGATTCTACGACTCATGGAACAGCTGGTATACCCCAACCCTGCAGCATACAGGGAGAAGCTAATTCGGTTTTCTTCACTTAACCATACAAATTATTCTCAGTTGGCACTAAAAGCGAGTCAGCTGATGGAGCAAACCAAGTTGAGTGAACTTCGTTCTAGCATTGCCAGAAGTCTTTCTGAGTTGGAGATGTTTACTGAGGATGGTGAAACCATGGATACTCCGAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCACCTTTGGCAGTCGAAGATGCCCTTGTTGGTCTGTTTGATCATGGTGATCACACCCTTCAGAGGCGGGTTGTCGAAAGTTATGTACGCCGGTTATACCAGCCCTACCTTGTAAAGGGGAGTGTCAGGATGCAGTGGCACAGATCAGGTCTAATAGCTTCATGGGAGTTCTCGGAAGAACCTGTTGAGAGGAAACATGAGATTGAAGATCAATCAGTTAATAAAACATTCCTTCAGAAACACGAGGAGAGGAAATGGGGAGTCATGGTTATAATAAAATCTCTTCACTTTCTTCCAGCAATTATTAGTGGTGCACTGAAGGAAATGTCGCATAACCTTCATGAAGCAACTCCAAATGGATCTACTGAACCAAGTGGCTTTGGTAATATGATGCATATTGCTTTGGTTGGCATCAACAATCAGATGAGTCTGCTACAGGATAGTGGCGACGAGGATCAGGCTCAAGAGAGAATTAAGAAGTTAGCCAAAATACTTAAAGAGCAAGGACTAGCCTCCAGTCTACATGGTGCAGGTGTCACAGTAATTAGTTGTATCATACAAAGGGATGAAGGGCGGCCCCCTATGAGGCACTCCTTCCACTGGTCATCAGAGAAACTCTATTTTGAAGAAGAACCCTTATTGCGTCACCTAGAACCTCCTCTATCTATTTATCTTGAATTGGACAAGCTTAAGGGCTATGAGAATATCCAGTATACCCCATCCCGTGACCGTCAATGGCACCTGTACACCGTTGTAGACAAGCCGCTACCGATCCAAAGGATGTTCCTCAGAACACTTGTAAGGCAGCCCACAACAAATGAAGGGTTCTCTGGATTTCAACGGCTAGACATCGCAACAGCTAGTAACCAATTGGCTTTATCATTTACTTCGAGGAGCATCTTGAGGTCCTTATCAACAGCAATGGAAGAGCTGGAATTGAATGCGCACAATGCCACTGTCAAATCTGACCACACCCATATGTATCTTTATATCTTACGTGAGCAACAAATTAATGATATGCTGCCTTACCACAAGAGAGTTGATTTAGATGCTCAACAAGAAGAAACTGTGGTGGAGGCCATCCTGGAAGAACTTGCACGTGAAATCCACGCATCAGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGTTGTGGATGGCATCCTCTGGACAGGCAAACGTTGCTTGGAGGGTTGTCGTGACAAATGTGACCGGTCATACCTGCACTGTACATATATATCGGGAACAAGAAGATACTAGCAAACAGAGAGTGGTTTACCATTCAGTCTCTGTGAAGGGTCCCCTGCATGGTGTACCAGTGAATGAGCAATATCAGCCTTTGGGGATTATTGACCGAAAGCGTCTGTTGGCCAGGAGAACTAACACCACTTACTGCTATGATTTTCCACTGGCGTTTGAGACGGCCTTGGAACAGTCATGGGCATCCCAGTCACCAAGTGTTAACAAACTAAAGGGTAAAATTCTCAAGGTCACAGAGCTCAAATTCGCTGACCAAAAAGGCAGCTGGGGTACTCCTCTCATTACCGTAGAGCGTCCACCTGGACTCAATGATGTTGGTATGATAGCATGGTTTATGGAAATGTCTACCCCTGAGTTCCCTTCTGGAAGGAAGATATTGGTTGTTGCAAATGATGTGACCTACAAAGCTGGATCTTTTGGTCCAAGAGAGGATGCATTCTTCTTTGCAGTAACTGAGCTTGCTTGTGCTGAGAAGTTGCCTCTAATATACTTGGCAGCAAACTCAGGTGCTCGTATTGGGGTTGCTGAAGAAGTCAAATCCTGTTTTAAAGTGGGCTGGTCTGATGAATCAAGCCCTGAACGTGGTTTCCAATATGTGTATTTAACCTCTGAGGACTATGCTCGGATTGGATCTTCTGTGATAGCGCATGAAATAAAGCTATCAAGTGGTGAAACCAGATGGGTGATAGATACCATTGTCGGAAAGGAGGATGGCTTGGGGGTTGAGAGCTTGACTGGAAGTGGGGCAATTGCTGGTGCATATTCGAGGGCATATAAGGAAACCTTTACCTTAACATATGTGACTGGCAGAACTGTAGGTATAGGTGCTTATCTTGCAAGGCTTGGGATGCGGTGCATACAGAGGCTTGATCAGCCCATCATTTTAACTGGTTTCTCTGCATTAAACAAACTACTTGGTCGGGAGGTGTACAGCTCCCATATGCAACTTGGAGGACCTAAAATTATGGGAACTAATGGGGTTGTCCATCTAACGGTAGCTGATGATCTTGAAGGAATATCTGCTATTCTAAAGTGGCTGAGCTATGTTCCTCCTCATGTTGGTGGCCCACTCCCCATTTCGATGCCCTTGGATCCTCCAGAAAGGCCCGTGGAGTATTGCCCGGAGAATTCATGTGATCCTCGAGCTGCGATTTCTGGTGCTCTGAATGGTAATGGGAACTGGATGGGGGGTATATTTGATAAAGACAGCTTTGTTGAGACGCTAGAAGGCTGGGCAAGGACGGTGGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTGGGAATTGTTGCTGTTGAGACACAGACTGTGATGCAAATTATCCCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGACAAGTATGGTTTCCTGATTCTGCTACTAAGACTGCCCAAGCATTATTAGATTTCAACAGAGAAGGACTCCCACTTTTCATTCTTGCCAACTGGAGAGGCTTTTCTGGTGGGCAGAGAGACCTTTTTGAAGGGATTCTTCAGGCTGGATCAACCATTGTGGAGAACCTTAGGACTTACAAACAGCCTGTTTTTGTATTCATCCCGATGATGGGGGAGCTACGTGGTGGAGCATGGGTGGTTGTTGATAGCCGGATAAATCCAGACCATATTGAAATGTATGCTGATCGAACGGCTAGAGGAAATGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTTAGGAACAAGGAATTGCTGGAGTGCATGGGCAGGCTTGATCAACAGCTGATCCAACTAAAGACTCAGCTTCAGGAAGCCAGGAGCTGTGAGGCAAATGAAAAGGTTGAGTCCCTACAGTTTCAAATACGATCCCGCGAAAAACAGCTTTTGCCTGTCTACACTCAGATAGCTACCAAGTTTGCAGAACTTCATGATACTTCTCTGAGGATGGCTGCAAAAGGGGTAATCAGAGGAGTTCTGGAATGGGCCAGCTCTCGATCTTTCTTCTACAAGAGATTGCGTCGGAGAATTGCCGATGAGTCACTCATCAAGATAGTGAGAGATGCTGCTGGTGAACAGCTGTCACATAAATCTGCTATGGATTTGATCAAGAACTGGTTTTTGAGTTCTGATGTTTCAAGAGGCAAAGAAGATGCTTGGGAGGATGATGAAACTTTCTTTAGATGGAAGGATGATCAAACAAATTATGAGGGCAAGCTAAAAGAACTTCGGGTCCAGAAGGTATTGCTTCAATTGGCAACCATTGGCAACTCAGCTTCTGATTTGCAAGCCCTACCCCAAGGTCTTGCCGCCCTTCTAAGCAAGGTTGAGCCATCAAGCCGATCATTGTTGGTTGAGGAACTCCGGAAGGTGCTTGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2269

Amino Acids

252.89

Weight (kDa)

6.03

Isoelectric Point (pI)

43.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	49 - 168	5.1e-28	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	216 - 398	6.2e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	217 - 368	1.4e-06	ATP-grasp domain
ATP-grasp_3	PF02655	223 - 370	1.7e-07	ATP-grasp domain
Biotin_carb_C	PF02785	445 - 551	2.3e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	567 - 682	4.3e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	693 - 755	5.5e-09	Biotin-requiring enzyme
ACC_central	PF08326	756 - 1499	3.1e-178	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1601 - 2152	8.7e-150	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3446
AarI	CACCTGC	4 cut(s)	109, 1099, 2200, 3695
AatII	GACGTC	1 cut(s)	1265
Acc16I	TGCGCA	1 cut(s)	4119
Acc36I	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
AccB1I	GGYRCC	3 cut(s)	97, 927, 3912
AccB7I	CCANNNNNTGG	1 cut(s)	1361
AccBSI	CCGCTC	1 cut(s)	576
AccI	GTMKAC	6 cut(s)	2934, 3694, 3887, 3930, 4793, 6321
AccII	CGCG	3 cut(s)	833, 1941, 6300
AccIII	TCCGGA	1 cut(s)	6789
AclI	AACGTT	1 cut(s)	4368
AcoI	YGGCCR	4 cut(s)	255, 261, 699, 4557
AcsI	RAATTY	8 cut(s)	591, 1197, 1551, 1557, 1569, 4668, 4691, 5575
AcvI	CACGTG	1 cut(s)	4274
AcyI	GRCGYC	1 cut(s)	1262
AdeI	CACNNNGTG	2 cut(s)	1131, 4646
AflII	CTTAAG	1 cut(s)	3863
AflIII	ACRYGT	3 cut(s)	394, 583, 1486
AgeI	ACCGGT	1 cut(s)	4400
AhlI	ACTAGT	1 cut(s)	1376
AjuI	GAANNNNNNNTTGG	2 cut(s)	2329, 2361
AleI	CACNNNNGTG	1 cut(s)	903
AloI	GAACNNNNNNTCC	6 cut(s)	2460, 2492, 5019, 5051, 6642, 6674
Alw21I	GWGCWC	8 cut(s)	1119, 1516, 2874, 3178, 3490, 4689, 4960, 5611
Alw26I	GTCTC	9 cut(s)	502, 968, 1512, 2075, 4480, 4601, 5660, 5741, 5937
Alw44I	GTGCAC	2 cut(s)	3174, 3486
AlwNI	CAGNNNCTG	7 cut(s)	1043, 1127, 1396, 4478, 4901, 6249, 6704
Ama87I	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
Aor13HI	TCCGGA	1 cut(s)	6789
ApaLI	GTGCAC	2 cut(s)	3174, 3486
ApoI	RAATTY	8 cut(s)	591, 1197, 1551, 1557, 1569, 4668, 4691, 5575
AseI	ATTAAT	2 cut(s)	536, 4185
AsiGI	ACCGGT	1 cut(s)	4400
Asp700I	GAANNNNTTC	2 cut(s)	3079, 5958
AspLEI	GCGC	3 cut(s)	2823, 4120, 5095
AsuC2I	CCSGG	2 cut(s)	698, 5570
AsuII	TTCGAA	1 cut(s)	1573
AvaI	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
AvaII	GGWCC	8 cut(s)	84, 748, 950, 4080, 4487, 4868, 5402, 6658
AxyI	CCTNAGG	2 cut(s)	5817, 5993
BaeGI	GKGCMC	2 cut(s)	3178, 3490
BaeI	ACNNNNGTAYC	2 cut(s)	5130, 5163
BalI	TGGCCA	3 cut(s)	257, 263, 4559
BamHI	GGATCC	2 cut(s)	1351, 5539
BanI	GGYRCC	3 cut(s)	97, 927, 3912
BanII	GRGCYC	3 cut(s)	1516, 2668, 4689
BarI	GAAGNNNNNNTAC	6 cut(s)	549, 581, 846, 878, 1911, 1943
BauI	CACGAG	1 cut(s)	3416
BbrPI	CACGTG	1 cut(s)	4274
BbsI	GAAGAC	1 cut(s)	279
Bbv12I	GWGCWC	8 cut(s)	1119, 1516, 2874, 3178, 3490, 4689, 4960, 5611
BceAI	ACGGC	3 cut(s)	4031, 4626, 6132
BcgI	CGANNNNNNTGC	2 cut(s)	983, 1017
BciVI	GTATCC	1 cut(s)	3115
BclI	TGATCA	8 cut(s)	310, 871, 3214, 3223, 5317, 6202, 6531, 6619
BcnI	CCSGG	2 cut(s)	698, 5570
BcoDI	GTCTC	9 cut(s)	502, 968, 1512, 2075, 4480, 4601, 5660, 5741, 5937
BcuI	ACTAGT	1 cut(s)	1376
BfmI	CTRYAG	7 cut(s)	102, 960, 1888, 2946, 3608, 5262, 6278
BfoI	RGCGCY	1 cut(s)	2824
BfrI	CTTAAG	1 cut(s)	3863
BfuAI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
BfuI	GTATCC	1 cut(s)	3115
BlpI	GCTNAGC	1 cut(s)	5486
BmcAI	AGTACT	1 cut(s)	1079
Bme18I	GGWCC	8 cut(s)	84, 748, 950, 4080, 4487, 4868, 5402, 6658
BmeT110I	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
BmrI	ACTGGG	2 cut(s)	1115, 4630
BmuI	ACTGGG	2 cut(s)	1115, 4630
BpiI	GAAGAC	1 cut(s)	279
BplI	GAGNNNNNCTC	2 cut(s)	5877, 5909
BpmI	CTGGAG	5 cut(s)	1467, 3673, 5529, 5938, 6203
Bpu10I	CCTNAGC	3 cut(s)	441, 465, 1797
Bpu1102I	GCTNAGC	1 cut(s)	5486
Bpu14I	TTCGAA	1 cut(s)	1573
BpuEI	CTTGAG	5 cut(s)	1796, 3621, 4096, 4658, 6155
BpuMI	CCSGG	2 cut(s)	698, 5570
BsaAI	YACGTR	5 cut(s)	61, 197, 4172, 4274, 6047
BsaBI	GATNNNNATC	2 cut(s)	2058, 6155
BsaHI	GRCGYC	1 cut(s)	1262
BsaI	GGTCTC	1 cut(s)	5937
BsaWI	WCCGGW	2 cut(s)	4400, 6789
BsaXI	ACNNNNNCTCC	6 cut(s)	949, 979, 3426, 3456, 6045, 6075
Bse118I	RCCGGY	2 cut(s)	3264, 4400
Bse21I	CCTNAGG	2 cut(s)	5817, 5993
Bse3DI	GCAATG	3 cut(s)	2241, 3066, 4101
Bse8I	GATNNNNATC	2 cut(s)	2058, 6155
BseAI	TCCGGA	1 cut(s)	6789
BseJI	GATNNNNATC	2 cut(s)	2058, 6155
BseMI	GCAATG	3 cut(s)	2241, 3066, 4101
BseSI	GKGCMC	2 cut(s)	3178, 3490
BseYI	CCCAGC	2 cut(s)	4713, 5679
BsgI	GTGCAG	3 cut(s)	383, 3723, 4396
Bsh1236I	CGCG	3 cut(s)	833, 1941, 6300
BshNI	GGYRCC	3 cut(s)	97, 927, 3912
BshTI	ACCGGT	1 cut(s)	4400
BsiHKAI	GWGCWC	8 cut(s)	1119, 1516, 2874, 3178, 3490, 4689, 4960, 5611
BsiHKCI	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
BsiSI	CCGG	6 cut(s)	698, 3265, 4401, 5570, 6076, 6790
BslFI	GGGAC	7 cut(s)	70, 84, 820, 1729, 2448, 4473, 6259
BsmAI	GTCTC	9 cut(s)	502, 968, 1512, 2075, 4480, 4601, 5660, 5741, 5937
BsmBI	CGTCTC	2 cut(s)	4601, 5660
BsmFI	GGGAC	7 cut(s)	70, 84, 820, 1729, 2448, 4473, 6259
BsmI	GAATGC	4 cut(s)	1182, 2498, 4120, 4883
Bso31I	GGTCTC	1 cut(s)	5937
BsoBI	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
Bsp119I	TTCGAA	1 cut(s)	1573
Bsp1286I	GDGCHC	9 cut(s)	1119, 1516, 2668, 2874, 3178, 3490, 4689, 4960, 5611
Bsp13I	TCCGGA	1 cut(s)	6789
Bsp1407I	TGTACA	4 cut(s)	1056, 3918, 4417, 5376
Bsp1720I	GCTNAGC	1 cut(s)	5486
Bsp19I	CCATGG	4 cut(s)	258, 569, 2308, 3117
BspEI	TCCGGA	1 cut(s)	6789
BspFNI	CGCG	3 cut(s)	833, 1941, 6300
BspHI	TCATGA	4 cut(s)	1459, 2056, 3514, 6355
BspMAI	CTGCAG	2 cut(s)	106, 2950
BspMI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
BspQI	GCTCTTC	1 cut(s)	4095
BspT104I	TTCGAA	1 cut(s)	1573
BspT107I	GGYRCC	3 cut(s)	97, 927, 3912
BspTI	CTTAAG	1 cut(s)	3863
BspTNI	GGTCTC	1 cut(s)	5937
BsrBI	CCGCTC	1 cut(s)	576
BsrDI	GCAATG	3 cut(s)	2241, 3066, 4101
BsrFI	RCCGGY	2 cut(s)	3264, 4400
BsrGI	TGTACA	4 cut(s)	1056, 3918, 4417, 5376
BssAI	RCCGGY	2 cut(s)	3264, 4400
BssNAI	GTATAC	2 cut(s)	2935, 3888
BssNI	GRCGYC	1 cut(s)	1262
BssSI	CACGAG	1 cut(s)	3416
BssT1I	CCWWGG	9 cut(s)	127, 258, 569, 1691, 2308, 3117, 4614, 5535, 6724
Bst1107I	GTATAC	2 cut(s)	2935, 3888
Bst2BI	CACGAG	1 cut(s)	3416
Bst6I	CTCTTC	5 cut(s)	2716, 2757, 3125, 3462, 4095
BstACI	GRCGYC	1 cut(s)	1262
BstAFI	CTTAAG	1 cut(s)	3863
BstAPI	GCANNNNNTGC	3 cut(s)	461, 2443, 4370
BstAUI	TGTACA	4 cut(s)	1056, 3918, 4417, 5376
BstBAI	YACGTR	5 cut(s)	61, 197, 4172, 4274, 6047
BstBI	TTCGAA	1 cut(s)	1573
BstDSI	CCRYGG	5 cut(s)	258, 569, 2308, 3117, 5556
BstEII	GGTNACC	1 cut(s)	692
BstENI	CCTNNNNNAGG	2 cut(s)	2196, 3287
BstFNI	CGCG	3 cut(s)	833, 1941, 6300
BstH2I	RGCGCY	1 cut(s)	2824
BstHHI	GCGC	3 cut(s)	2823, 4120, 5095
BstMAI	GTCTC	9 cut(s)	502, 968, 1512, 2075, 4480, 4601, 5660, 5741, 5937
BstNSI	RCATGY	6 cut(s)	398, 587, 648, 1490, 1610, 2040
BstPI	GGTNACC	1 cut(s)	692
BstSFI	CTRYAG	7 cut(s)	102, 960, 1888, 2946, 3608, 5262, 6278
BstSLI	GKGCMC	2 cut(s)	3178, 3490
BstSNI	TACGTA	2 cut(s)	61, 197
BstUI	CGCG	3 cut(s)	833, 1941, 6300
BstV2I	GAAGAC	1 cut(s)	279
BstX2I	RGATCY	9 cut(s)	435, 806, 1351, 2845, 3163, 3533, 4859, 5078, 5539
BstXI	CCANNNNNNTGG	1 cut(s)	1422
BstYI	RGATCY	9 cut(s)	435, 806, 1351, 2845, 3163, 3533, 4859, 5078, 5539
BstZ17I	GTATAC	2 cut(s)	2935, 3888
Bsu36I	CCTNAGG	2 cut(s)	5817, 5993
BsuI	GTATCC	1 cut(s)	3115
BtgI	CCRYGG	5 cut(s)	258, 569, 2308, 3117, 5556
BtgZI	GCGATG	3 cut(s)	1763, 1956, 4009
BtsI	GCAGTG	3 cut(s)	411, 919, 3314
BveI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
CaiI	CAGNNNCTG	7 cut(s)	1043, 1127, 1396, 4478, 4901, 6249, 6704
CciI	TCATGA	4 cut(s)	1459, 2056, 3514, 6355
CfoI	GCGC	3 cut(s)	2823, 4120, 5095
Cfr10I	RCCGGY	2 cut(s)	3264, 4400
CseI	GACGC	6 cut(s)	822, 3806, 4538, 4730, 5677, 6434
CspAI	ACCGGT	1 cut(s)	4400
CspCI	CAANNNNNGTGG	8 cut(s)	1264, 1299, 1625, 1660, 5673, 5708, 5964, 5999
DraI	TTTAAA	2 cut(s)	1369, 4996
DraIII	CACNNNGTG	2 cut(s)	1131, 4646
EaeI	YGGCCR	4 cut(s)	255, 261, 699, 4557
Eam1104I	CTCTTC	5 cut(s)	2716, 2757, 3125, 3462, 4095
EarI	CTCTTC	5 cut(s)	2716, 2757, 3125, 3462, 4095
Ecl136II	GAGCTC	2 cut(s)	1514, 4687
Eco105I	TACGTA	2 cut(s)	61, 197
Eco130I	CCWWGG	9 cut(s)	127, 258, 569, 1691, 2308, 3117, 4614, 5535, 6724
Eco147I	AGGCCT	1 cut(s)	1687
Eco24I	GRGCYC	3 cut(s)	1516, 2668, 4689
Eco31I	GGTCTC	1 cut(s)	5937
Eco47I	GGWCC	8 cut(s)	84, 748, 950, 4080, 4487, 4868, 5402, 6658
Eco53kI	GAGCTC	2 cut(s)	1514, 4687
Eco72I	CACGTG	1 cut(s)	4274
Eco81I	CCTNAGG	2 cut(s)	5817, 5993
Eco88I	CYCGRG	4 cut(s)	1509, 2624, 2736, 5589
Eco91I	GGTNACC	1 cut(s)	692
EcoICRI	GAGCTC	2 cut(s)	1514, 4687
EcoNI	CCTNNNNNAGG	2 cut(s)	2196, 3287
EcoO109I	RGGNCCY	6 cut(s)	84, 1877, 2330, 4080, 4487, 5402
EcoO65I	GGTNACC	1 cut(s)	692
EcoRI	GAATTC	1 cut(s)	5575
EcoT14I	CCWWGG	9 cut(s)	127, 258, 569, 1691, 2308, 3117, 4614, 5535, 6724
EcoT22I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
EcoT38I	GRGCYC	3 cut(s)	1516, 2668, 4689
ErhI	CCWWGG	9 cut(s)	127, 258, 569, 1691, 2308, 3117, 4614, 5535, 6724
Esp3I	CGTCTC	2 cut(s)	4601, 5660
FaqI	GGGAC	7 cut(s)	70, 84, 820, 1729, 2448, 4473, 6259
FauI	CCCGC	3 cut(s)	2522, 3234, 6305
FauNDI	CATATG	4 cut(s)	2146, 4152, 5245, 5388
FbaI	TGATCA	8 cut(s)	310, 871, 3214, 3223, 5317, 6202, 6531, 6619
FblI	GTMKAC	6 cut(s)	2934, 3694, 3887, 3930, 4793, 6321
FriOI	GRGCYC	3 cut(s)	1516, 2668, 4689
FspAI	RTGCGCAY	1 cut(s)	4119
FspI	TGCGCA	1 cut(s)	4119
GlaI	GCGC	3 cut(s)	2822, 4119, 5094
GsaI	CCCAGC	2 cut(s)	4717, 5683
GsuI	CTGGAG	5 cut(s)	1467, 3673, 5529, 5938, 6203
HaeII	RGCGCY	1 cut(s)	2824
HapII	CCGG	6 cut(s)	698, 3265, 4401, 5570, 6076, 6790
HgaI	GACGC	6 cut(s)	822, 3806, 4538, 4730, 5677, 6434
HhaI	GCGC	3 cut(s)	2823, 4120, 5095
Hin1I	GRCGYC	1 cut(s)	1262
Hin6I	GCGC	3 cut(s)	2821, 4118, 5093
HinP1I	GCGC	3 cut(s)	2821, 4118, 5093
HincII	GTYRAC	1 cut(s)	4651
HindII	GTYRAC	1 cut(s)	4651
HindIII	AAGCTT	4 cut(s)	2314, 2377, 3860, 5711
HpaI	GTTAAC	1 cut(s)	4651
HpaII	CCGG	6 cut(s)	698, 3265, 4401, 5570, 6076, 6790
Hpy99I	CGWCG	3 cut(s)	1267, 3626, 6450
HpyCH4IV	ACGT	8 cut(s)	60, 196, 1262, 4171, 4273, 4368, 5029, 6046
HpySE526I	ACGT	8 cut(s)	60, 196, 1262, 4171, 4273, 4368, 5029, 6046
Hsp92I	GRCGYC	1 cut(s)	1262
HspAI	GCGC	3 cut(s)	2821, 4118, 5093
KflI	GGGWCCC	1 cut(s)	4487
Kpn2I	TCCGGA	1 cut(s)	6789
Ksp22I	TGATCA	8 cut(s)	310, 871, 3214, 3223, 5317, 6202, 6531, 6619
KspAI	GTTAAC	1 cut(s)	4651
LguI	GCTCTTC	1 cut(s)	4095
MaeII	ACGT	8 cut(s)	60, 196, 1262, 4171, 4273, 4368, 5029, 6046
MbiI	CCGCTC	1 cut(s)	576
MfeI	CAATTG	7 cut(s)	669, 1593, 2216, 2449, 4043, 5196, 6677
MflI	RGATCY	9 cut(s)	435, 806, 1351, 2845, 3163, 3533, 4859, 5078, 5539
MhlI	GDGCHC	9 cut(s)	1119, 1516, 2668, 2874, 3178, 3490, 4689, 4960, 5611
MlsI	TGGCCA	3 cut(s)	257, 263, 4559
MluNI	TGGCCA	3 cut(s)	257, 263, 4559
MmeI	TCCRAC	9 cut(s)	969, 1012, 1629, 1944, 2360, 3069, 5128, 6241, 6428
Mox20I	TGGCCA	3 cut(s)	257, 263, 4559
Mph1103I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
MroI	TCCGGA	1 cut(s)	6789
MroXI	GAANNNNTTC	2 cut(s)	3079, 5958
MscI	TGGCCA	3 cut(s)	257, 263, 4559
Msp20I	TGGCCA	3 cut(s)	257, 263, 4559
MspA1I	CMGCKG	7 cut(s)	494, 2006, 2929, 3028, 4713, 6211, 6505
MspCI	CTTAAG	1 cut(s)	3863
MspI	CCGG	6 cut(s)	698, 3265, 4401, 5570, 6076, 6790
MunI	CAATTG	7 cut(s)	669, 1593, 2216, 2449, 4043, 5196, 6677
Mva1269I	GAATGC	4 cut(s)	1182, 2498, 4120, 4883
MvnI	CGCG	3 cut(s)	833, 1941, 6300
NciI	CCSGG	2 cut(s)	698, 5570
NcoI	CCATGG	4 cut(s)	258, 569, 2308, 3117
NdeI	CATATG	4 cut(s)	2146, 4152, 5245, 5388
NsbI	TGCGCA	1 cut(s)	4119
NsiI	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
NspI	RCATGY	6 cut(s)	398, 587, 648, 1490, 1610, 2040
NspV	TTCGAA	1 cut(s)	1573
OliI	CACNNNNGTG	1 cut(s)	903
PaeI	GCATGC	1 cut(s)	648
PaeR7I	CTCGAG	3 cut(s)	1509, 2624, 5589
PagI	TCATGA	4 cut(s)	1459, 2056, 3514, 6355
PaqCI	CACCTGC	4 cut(s)	109, 1099, 2200, 3695
PasI	CCCWGGG	1 cut(s)	1880
PceI	AGGCCT	1 cut(s)	1687
PciI	ACATGT	3 cut(s)	394, 583, 1486
PciSI	GCTCTTC	1 cut(s)	4095
PctI	GAATGC	4 cut(s)	1182, 2498, 4120, 4883
PdmI	GAANNNNTTC	2 cut(s)	3079, 5958
PflMI	CCANNNNNTGG	1 cut(s)	1361
PfoI	TCCNGGA	2 cut(s)	802, 4257
PinAI	ACCGGT	1 cut(s)	4400
PmaCI	CACGTG	1 cut(s)	4274
PmlI	CACGTG	1 cut(s)	4274
Ppu21I	YACGTR	5 cut(s)	61, 197, 4172, 4274, 6047
PpuMI	RGGWCCY	4 cut(s)	84, 4080, 4487, 5402
PscI	ACATGT	3 cut(s)	394, 583, 1486
PshBI	ATTAAT	2 cut(s)	536, 4185
PsiI	TTATAA	1 cut(s)	3446
Psp124BI	GAGCTC	2 cut(s)	1516, 4689
Psp1406I	AACGTT	1 cut(s)	4368
Psp5II	RGGWCCY	4 cut(s)	84, 4080, 4487, 5402
PspCI	CACGTG	1 cut(s)	4274
PspEI	GGTNACC	1 cut(s)	692
PspFI	CCCAGC	2 cut(s)	4713, 5679
PspPPI	RGGWCCY	4 cut(s)	84, 4080, 4487, 5402
PspXI	VCTCGAGB	1 cut(s)	5589
PstI	CTGCAG	2 cut(s)	106, 2950
PstNI	CAGNNNCTG	7 cut(s)	1043, 1127, 1396, 4478, 4901, 6249, 6704
PsuI	RGATCY	9 cut(s)	435, 806, 1351, 2845, 3163, 3533, 4859, 5078, 5539
PvuII	CAGCTG	7 cut(s)	494, 2006, 2929, 3028, 4713, 6211, 6505
SacI	GAGCTC	2 cut(s)	1516, 4689
SapI	GCTCTTC	1 cut(s)	4095
ScaI	AGTACT	1 cut(s)	1079
SduI	GDGCHC	9 cut(s)	1119, 1516, 2668, 2874, 3178, 3490, 4689, 4960, 5611
SfcI	CTRYAG	7 cut(s)	102, 960, 1888, 2946, 3608, 5262, 6278
Sfr274I	CTCGAG	3 cut(s)	1509, 2624, 5589
SfuI	TTCGAA	1 cut(s)	1573
SinI	GGWCC	8 cut(s)	84, 748, 950, 4080, 4487, 4868, 5402, 6658
SlaI	CTCGAG	3 cut(s)	1509, 2624, 5589
SmlI	CTYRAG	9 cut(s)	1509, 1775, 2624, 3636, 3863, 4075, 4673, 5589, 6134
SmoI	CTYRAG	9 cut(s)	1509, 1775, 2624, 3636, 3863, 4075, 4673, 5589, 6134
SnaBI	TACGTA	2 cut(s)	61, 197
SpeI	ACTAGT	1 cut(s)	1376
SphI	GCATGC	1 cut(s)	648
SseBI	AGGCCT	1 cut(s)	1687
SspI	AATATT	1 cut(s)	477
SstI	GAGCTC	2 cut(s)	1516, 4689
StuI	AGGCCT	1 cut(s)	1687
StyI	CCWWGG	9 cut(s)	127, 258, 569, 1691, 2308, 3117, 4614, 5535, 6724
TaiI	ACGT	8 cut(s)	63, 199, 1265, 4174, 4276, 4371, 5032, 6049
TaqII	GACCGA	1 cut(s)	4557
TatI	WGTACW	5 cut(s)	1056, 1077, 3918, 4417, 5376
TauI	GCSGC	6 cut(s)	98, 928, 1748, 3751, 3940, 6737
TspGWI	ACGGA	1 cut(s)	2047
Van91I	CCANNNNNTGG	1 cut(s)	1361
Vha464I	CTTAAG	1 cut(s)	3863
VneI	GTGCAC	2 cut(s)	3174, 3486
VpaK11BI	GGWCC	8 cut(s)	84, 748, 950, 4080, 4487, 4868, 5402, 6658
VspI	ATTAAT	2 cut(s)	536, 4185
XagI	CCTNNNNNAGG	2 cut(s)	2196, 3287
XapI	RAATTY	8 cut(s)	591, 1197, 1551, 1557, 1569, 4668, 4691, 5575
XceI	RCATGY	6 cut(s)	398, 587, 648, 1490, 1610, 2040
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1128, 1169, 4289, 5393
XhoI	CTCGAG	3 cut(s)	1509, 2624, 5589
XmiI	GTMKAC	6 cut(s)	2934, 3694, 3887, 3930, 4793, 6321
XmnI	GAANNNNTTC	2 cut(s)	3079, 5958
ZraI	GACGTC	1 cut(s)	1263
ZrmI	AGTACT	1 cut(s)	1079
Zsp2I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.