Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

21797770 .. 21810908

Gene Structure 13139 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1178000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6813 bp

ATGGTTTCACAAAGCATGTCAGAAGCCCAGAGGAGATTGGTGACAGTGCCCAGTTTCCATCGTGGAAATGGGTACGTAAATGGGGTACTCAGGCATCCTGCTACAGCATCTAAAGTTGATGAATTCTGCTATGCCCTTGGTGGGAAGAAGCCAATTCATAGCATTTTAATTGCAAACAATGGAATGGCAGCTGTCAAGTTTATTCGTAGTGTGAGGACATGGTCTTATGAAACATTTGGTACAGAGAAGGCTGTTTTATTGGTGGCAATGGCCACACCAGAGGACATGAGAATCAATGCAGAACACATCAGAATTGCCGATCAGTTTGTGGAGGTTCCTGGTGGAACTAACAATAATAATTATGCCAATGTACAACTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCCTCAGAGAATCCTGAGCTTCCAGATGCATTGGATGCAAAGGGAATTGTATTTCTTGGGCCACCATCTATATCTATGGCAGCATTAGGAGATAAAATCGGTTCATCATTAATTGCTCAGGCTGCCAACGTACCTACTCTTCCATGGAGTGGATCACATGTGAAAATTCCTTCAGAGAGCTGCTTGGTTACAATCCCAGATGAAGTATATAGGGAAGCATGTGTTTATACAACAGAGGAAGCAGTTGCAAGTTGTCAAGTTGTAGGTTACCCAGCAATGATTAAGGCTTCATGGGGTGGAGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAGGTAAGGGCATTGTTCAAGCAAGTTCAGGGTGAAGTTCCTGGATCTCCAATTTTTATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAGGTACAGTTACTGTGTGATCAGCATGGAAATGTTGCAGCTTTTCATAGCCGTGACTGCAGTGTTCAAAGGCGCCACCAGAAGATTATTGAGGAGGGTCCAATTACTGTAGCTCCTATAGAGACGGTAAAAAAGTTGGAGCAATCAGCTCGAAGGTTGGCTAAATCTGTTAATTACGTTGGAGCAGCTACTGTTGAGTATCTCTACAGTATGGAAACTGGCGAGTACTATTTCTTAGAGCTGAACCCTCGATTACAGGTGGAGCACCCAGTCACTGAGTGGATAGCTGAAATAAATCTCCCAGCAGCCCAAGTTGTAGTTGGGATGGGCATACCTCTCTGGCAAATCCCTGAAATAAGGCGATTTTATGGAATGGAACATGGTGGCGGATATGATGCTTGGAGAAAAACTTCAGCTAGTGCTTCCCCATTCGATTTTGACAAGGCAGAATCTACAAGGCCAAAGGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAGCCTACCAGTGGGAAAGTACAGGAGTTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCATACTTTTCTGTTAAGTCTGGAGGGGGCATTCATGAATTCTCGGACTCTCAGTTTGGACATGTTTTTGCATTTGGGGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTTGATTATTCAATAGATCTTCTACATGCCTCAGACTACAGAGAGAATAAAATCCACACAGGTTGGTTGGATAGTAGAATCGCCATGCGAGTTAGAGCAGAAAGGCCTCCTTGGTATCTTTCTGTAGTTGGAGGGACTCTGTTTAAAGCATCTACCAGTAGTGCAGCAATGGTTTCAGATTATGTCGGTTATCTTGAGAAAGGGCAAATTCCACCCAAGCATATATCACTTGTCCATGCACAAGTATCATTGAACATTGAAGGGAGCAAATACACGATTGACATGGTGAGAGGGGGTCCTGGAAGCTACAGATTGAGGATGAATAAATCAGAGATTGAAGCAGAGATACATACTTTACGTGATGGGGGTTTGTTGATGCAGTTGGACGGAAACAGTCACATTATATACGCAGAGGAAGAAGCAGCTGGAACTCGCCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGACCATGACCCATCAAAGTTAATAGCAGAGACACCATGCAAGCTGCTGAGGTACTTGGTTGCTGATGATAGTCATGTTGATGCTGACACACCATATGCTGAGGTTGAGGTTATGAAGATGTGTATGCCTCTTCTTTCACCTGCTTCTGGAGCTATCCACTTTAGAATGTCTGAAGGTCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCGGCTGTAAGAAAGGCAGAACCTTTCCATGGGAGCTTCCCGGTTCTGGGGCCCCCAACTGCAATTTCTGGTAAAGTTCACCAGAGATGTGCTGCTAGCATAACCGCAGCCCAAATGATTCTTGCTGGTTATGAGCATAACATTGACGAAGTAGTGCAAAACTTGCTCACTTGCCTAGACAATCCTGAACTCCCTTTCCTTCAATGGCAAGAGTGCTTTGCTGTTCTAGCAACCCGCCTACCCAAAGATCTTAAAAATGAATTGGAATCAAAATTTAAGGAGTTTGAGTTGATTTCTAGTTCTCAGAATGTTGAGTTCCCTGCTAAGTTGTTGCGCGTTGTTCTTGAGGCCCATCTATTCTCCTGTCCTGATAAAGAGAAAGGAGCCCAAGAAAGGCTTGTTGAGCCTTTGATAAGCCTTGTGAAGTCTTATGAGGGTGGAAGAGAAAGTCATGCCCGAGTTATCGTCCATTCCCTTTTTGAAGAGTACCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTAATTGAACGCCTTCGACTTCAGTATAAAAAAGATCTATTGAAGATTGTGGACATTGTGCTTTCTCACCAGGGTGTCAAGAATAAAAATAAGTTGATACTACGACTCATGGAACAACTGGTCTACCCCAACCCTGCTGCGTACAGGGAAAAGCTAATTCGGTTTTCTCAACTTAACCATACAAGCTATTCTGAGTTGGCACTTAAGGCAAGTCAACTGATGGAGCAAACCAAGTTGAGTGAACTTCGTTCCAGCATTGCCAGAAGCCTTTCTGAGTTAGAGATGTTTACCGAGGATGGTGAAACCATGGATACTCCTAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGCGCTCCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTATTTGATCACAGTGATCACACACTTCAGAGGCGGGTTGTGGAGAGCTATGTGCGTCGGTTATACCAGCCATATCTTTTAAAGGGAAGTGTGAGGATGCAGTGGCAAAGATCTGGTCTTATGGCTTCCTGGGAGTTCTTGGATGAACATACCGAGAGAAAAACTGCGAATGAAGATCAATCTTCTGATAAATCAATTGAGAAATACAACGAGAGAAAATGGGGAGTCATGGTTATTATAAAATCCCTTCAATTTCTGCCAGCAGTTATTAATGCTGCACTGAAGGAAATGTCTCATCAACTTAATGAATCAATTCCAAATGGATCTAGTGAACCAAGTGGCTTTGGTAATATGATGCATATTGCTTTAGTTGGCATCAACAATCAGATGAGTTTACTTCAGGATAGTGGTGATGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATTCTTAAAGAACAGGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCAGGGTAATTAGTTGCATCATACAAAGAGATGAAGGGCGAGCTCCTATGAGGCATTCCTTCCACTGGTCATCAGAAAAACTCTATTACGAGGAAGAACCTTTATTGCGTCATCTGGAGCCTCCTCTATCCATTTACCTTGAATTGGACAAGCTTAAAGGTTATGAGAATGTACAGTATACCCCATCCCGGGACCGTCAATGGCACTTGTACAGCGTTGTAGACAAGCCACAACCAACCAAAAGGATGTTCCTCAGAACACTTGTAAGGCAGCCCACTTCAAATGATCTGTTCGCTGGATTTCAAAGGCTAGACATGGAAGCAGCTAGTAAGCAATGGGCTTTGTCATTTACTTCAAGGAGCATTTTGGGGTCCTTATTGACAGCAATGGAAGAGTTGGAACTTAATGCACACAATGCCACTGTCAAATCTGACCATACTCATATGTACCTTTATATCTTACGTGAGCAACAAATTGATGATCTACTGCCTTACTCCAAGGGAGTTGGTTTAGATGCTGGACAAGAAGAAATTGTGGTGGAGGCGATCTTAGAAGAACTTGCACGTGAAATCCATGCATCTGTTGGTGTAAAGATGCATAGGTTGGGTGTTTGTGAATGGGAAGTGAAGTTGTGGATTGCATCCTCTGGGCAGGCAAATGTTTCTTGGAGGGTTGTCGTCACAAACGTGACTGGTCATACCTGCACTGTACATGTATATCGGGAACTAGAGGATACCAGCAAACAGAGGGTGGTATACCATTCAGCCTCTACACAGGCCCCCCTGCATGGTTTACCAGTAAATGCACAGTATCAGCCTTTGGGAGCTATTGACCGAAAACGTCTGGTGGCCAGGAGAACCAGCACCACTTACTGCTATGATTTTCCACTGGCATTTCAGACGGCCTTGGAACAGTCATGGGCATCCCAGTTGCCGGGTAGTAAAAAACCGAAAGATAAAATTCTAAGAGTCACTGAGCTCAAATTTTCTGACCAACAAGGCACCTGGGGCACTCCTCTTGTTGAAGTAGTTCGTCCACCTGGGCTCAATGACGTTGGCATGGTAGCCTGGTTTATGGAGATGTCTACCCCCGAGTTCCCTTCAGGAAGGAAGATATTGATTGTTTCAAATGACGTGACCTTCAAAGCTGGATCTTTTGGTCCAAGAGAGGATGCATTCTTCTTTGCAGTAACTGAACTTGCTTGTGCTAAGAAACTGCCCTTAATATACTTGGCAGCAAACTCAGGTGCCCGTATTGGGGTAGCTGAAGAAGTCAAATCCTGCTTTAAAGTTGGTTGGTCTGATGAATCGAGTCCTGAGCGTGGTTTTCAGTATGTGTATTTAACCTCTGAGGATTATGCTCGGATTGGATCATCTGTGATAGCACATGAATTAAAGCTGGCAAATGGAGAGACCCGATGGGTTATAGATACCATTGTTGGAAAGGATGATGGCTTAGGGGTTGAAAGCTTGACTGGGAGTGGGGCAATTGCCGGTGCATATTCGAGGGCATACAGGGAAACCTTTACCTTAACATATGTGACTGGAAGAACTGTAGGGATTGGTGCTTATCTTGCCCGGCTTGGGATGCGGTGCATACAGAGGCTTGATCAGCCCATTATTTTAACCGGTTTCTCTGCATTGAACAAACTTCTTGGCCGGGAGGTATACAGCTCTCACATGCAACTTGGAGGACCTAAAATTATGGGCACGAATGGGGTTGTCCATTTAACGGTTGCTGATGATCTTGAAGGAATATCTGCTATTTTGAAGTGGTTAAGTTATGTTCCTGCCCATGCTGGTGGGCCGCTCCCCATTTTGAGTCCCTTAGATCCTCCAGAAAGGCCTGTTGAGTACTGCCCTGAGAATTCATGCGATCCTCGTGCTGCTATTAGTGGTGCTTTAAATAGTAACGGGAAGTGGATGGGTGGTATTTTTGACAAAGACAGCTTTGTTGAGACGCTAGAAGGCTGGGCGAGGACGGTTGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTAGGAATAGTTGCTGTTGAGACACAGACGGTGATGCAAACCATACCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGGCAAGTGTGGTTTCCTGATTCTGCTAGTAAGACAGCCCAAGCGTTATTAGATTTCAACAGAGAAGAGCTCCCACTTTTCATTCTTGCCAACTGGAGAGGATTTTCTGGTGGGCAGAGGGACCTTTTCGAAGGGATCCTTCAAGCTGGATCAACCATTGTGGAGAACCTCAGGACTTACAAACAGCCCATATTTGTGTTCATCCCCATGATGGGTGAGCTACGCGGTGGAGCGTGGGTGGTCGTGGATAGCCGTATAAATCCAGACCATATTGAAATGTATGCTGATCAAACTGCTAGAGGTAACGTGCTTGAGCCAGAGGGAATGATCGAGATCAAGTTCAGGAACAAGGAGCTACTGGAGAGCATGGGCAGGCTTGACCAACAGCTGATCCAACTGAAGGCAAAACTTCAGGAAACCAAGAGCAGTGGGGCACATGAGATGGTTGAACCTCTTCAGCATCAGATACGATCACGTGAAAAACAGCTTTTGCCTGTCTACACTCAGATAGCCACTAGATTTGCGGAACTGCACGATACTTCTCTCAGGATGGCTGCAAAGGGAGTGATCAGAGAAGTTTTGGACTGGAACAGCTGTCGAGCTTTCTTCTATAAAAGATTGCGTCGGAGAATTTCCGAAGAGTCACTCATCAAGACAGTGAGAGATGCTGCTGGTGAACAGCTGTCGCATAAATCTGCTACAGATTTGATCCAGAACTGGTTTTTGAGTTCGGATATTCCGGGATGCAAAAAAGATGCTTGGGTGGACGACGAAATTTTCTTTAGATGGAAGGAAAGTCCGAAAAATTACGAGGATAAACTAAATGAGTTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAAATATTGGCGACTCAATTTCTGATTTGCAAGCCCTACCTCAAGGTCTTGCTGCCCTTCTAAGCAAGGTCGAGCCATCAAGCAGAGCGCTGTTGATTGATGAACTCCGAAAGGTGCTCGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2271

Amino Acids

253.14

Weight (kDa)

6.07

Isoelectric Point (pI)

41.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	52 - 170	4.8e-29	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	219 - 401	7.8e-45	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	220 - 371	1.3e-07	ATP-grasp domain
ATP-grasp_3	PF02655	227 - 373	1.3e-07	ATP-grasp domain
Biotin_carb_C	PF02785	448 - 554	2.3e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	570 - 685	4.1e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	695 - 758	1.6e-08	Biotin-requiring enzyme
ACC_central	PF08326	759 - 1501	6e-179	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1603 - 2154	2e-151	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	830, 3455
AarI	CACCTGC	2 cut(s)	2209, 3701
Acc36I	ACCTGC	4 cut(s)	2209, 3701, 4424, 5788
AccB1I	GGYRCC	3 cut(s)	933, 4715, 4961
AccB7I	CCANNNNNTGG	2 cut(s)	584, 1370
AccBSI	CCGCTC	1 cut(s)	5524
AccI	GTMKAC	8 cut(s)	2943, 3700, 3893, 3936, 4470, 4799, 5382, 6327
AccII	CGCG	2 cut(s)	2625, 6054
AciI	CCGC	9 cut(s)	1248, 2394, 2524, 3250, 5305, 5522, 6054, 6353, 6661
AcoI	YGGCCR	3 cut(s)	270, 4563, 5371
AcvI	CACGTG	2 cut(s)	4280, 6305
AcyI	GRCGYC	1 cut(s)	934
AdeI	CACNNNGTG	1 cut(s)	1140
AfeI	AGCGCT	2 cut(s)	3184, 6777
AflII	CTTAAG	1 cut(s)	3023
AflIII	ACRYGT	4 cut(s)	403, 592, 1495, 4426
AgeI	ACCGGT	1 cut(s)	5342
AjiI	CACGTC	1 cut(s)	4849
AjuI	GAANNNNNNNTTGG	4 cut(s)	2338, 2370, 3947, 3979
AleI	CACNNNNGTG	2 cut(s)	2892, 4400
AloI	GAACNNNNNNTCC	2 cut(s)	2469, 2501
Alw21I	GWGCWC	6 cut(s)	1128, 1525, 3760, 4695, 5901, 6807
Alw26I	GTCTC	7 cut(s)	977, 1521, 2084, 3513, 5120, 5666, 5747
AlwNI	CAGNNNCTG	4 cut(s)	874, 1052, 1136, 4907
Ama87I	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
Aor51HI	AGCGCT	2 cut(s)	3184, 6777
ApaI	GGGCCC	1 cut(s)	2343
ArsI	GACNNNNNNTTYG	4 cut(s)	1472, 1504, 3316, 3348
AseI	ATTAAT	2 cut(s)	545, 3486
AsiGI	ACCGGT	1 cut(s)	5342
Asp700I	GAANNNNTTC	6 cut(s)	1270, 2832, 3088, 3528, 4743, 6282
AspLEI	GCGC	4 cut(s)	936, 2625, 3185, 6778
AsuC2I	CCSGG	7 cut(s)	2330, 3904, 3905, 4650, 5293, 5375, 6570
AsuII	TTCGAA	1 cut(s)	5958
AsuNHI	GCTAGC	1 cut(s)	2384
AvaI	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
AvaII	GGWCC	9 cut(s)	757, 959, 1886, 3907, 4086, 4874, 5408, 5950, 6664
AxyI	CCTNAGG	2 cut(s)	5823, 5999
BaeGI	GKGCMC	6 cut(s)	51, 2343, 4727, 4966, 5426, 6265
BaeI	ACNNNNGTAYC	2 cut(s)	5136, 5169
BalI	TGGCCA	2 cut(s)	272, 4565
BamHI	GGATCC	2 cut(s)	1360, 5964
BanI	GGYRCC	3 cut(s)	933, 4715, 4961
BanII	GRGCYC	7 cut(s)	1525, 2343, 2677, 3760, 4695, 4761, 5901
BarI	GAAGNNNNNNTAC	4 cut(s)	558, 590, 1920, 1952
BauI	CACGAG	1 cut(s)	5595
BbrPI	CACGTG	2 cut(s)	4280, 6305
Bbv12I	GWGCWC	6 cut(s)	1128, 1525, 3760, 4695, 5901, 6807
BbvCI	CCTCAGC	2 cut(s)	2108, 2160
BceAI	ACGGC	3 cut(s)	897, 4632, 6066
BcgI	CGANNNNNNTGC	4 cut(s)	992, 1026, 5579, 5613
BciVI	GTATCC	2 cut(s)	3124, 4441
BclI	TGATCA	6 cut(s)	880, 3223, 3232, 5323, 6115, 6396
BcnI	CCSGG	7 cut(s)	2330, 3904, 3905, 4650, 5293, 5375, 6570
BcoDI	GTCTC	7 cut(s)	977, 1521, 2084, 3513, 5120, 5666, 5747
BfoI	RGCGCY	3 cut(s)	937, 3186, 6779
BfrI	CTTAAG	1 cut(s)	3023
BfuAI	ACCTGC	4 cut(s)	2209, 3701, 4424, 5788
BfuI	GTATCC	2 cut(s)	3124, 4441
BglII	AGATCT	4 cut(s)	1606, 2536, 2854, 3326
BmcAI	AGTACT	2 cut(s)	1088, 5570
Bme18I	GGWCC	9 cut(s)	757, 959, 1886, 3907, 4086, 4874, 5408, 5950, 6664
BmeT110I	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
BmgBI	CACGTC	1 cut(s)	4849
BmrI	ACTGGG	4 cut(s)	45, 1124, 4636, 5199
BmtI	GCTAGC	1 cut(s)	2388
BmuI	ACTGGG	4 cut(s)	45, 1124, 4636, 5199
BplI	GAGNNNNNCTC	2 cut(s)	5883, 5915
BpmI	CTGGAG	7 cut(s)	1476, 2229, 3679, 3851, 5535, 5944, 6209
Bpu10I	CCTNAGC	6 cut(s)	450, 552, 2108, 2160, 5031, 5170
Bpu14I	TTCGAA	1 cut(s)	5958
BpuEI	CTTGAG	5 cut(s)	1805, 2654, 3627, 6161, 6714
BpuMI	CCSGG	7 cut(s)	2330, 3904, 3905, 4650, 5293, 5375, 6570
BsaAI	YACGTR	5 cut(s)	76, 1949, 4178, 4280, 6305
BsaBI	GATNNNNATC	2 cut(s)	3633, 6161
BsaHI	GRCGYC	1 cut(s)	934
BsaI	GGTCTC	1 cut(s)	5120
BsaWI	WCCGGW	1 cut(s)	5342
BsaXI	ACNNNNNCTCC	8 cut(s)	958, 988, 2561, 2591, 3432, 3462, 6051, 6081
Bse118I	RCCGGY	2 cut(s)	5207, 5342
Bse21I	CCTNAGG	2 cut(s)	5823, 5999
Bse3DI	GCAATG	7 cut(s)	273, 717, 1764, 2250, 3075, 4055, 4107
Bse8I	GATNNNNATC	2 cut(s)	3633, 6161
BseJI	GATNNNNATC	2 cut(s)	3633, 6161
BseMI	GCAATG	7 cut(s)	273, 717, 1764, 2250, 3075, 4055, 4107
BseRI	GAGGAG	5 cut(s)	46, 968, 1587, 3828, 4719
BseSI	GKGCMC	6 cut(s)	51, 2343, 4727, 4966, 5426, 6265
BseYI	CCCAGC	4 cut(s)	706, 1162, 5685, 5828
BsgI	GTGCAG	5 cut(s)	1773, 3477, 3729, 4402, 6344
Bsh1236I	CGCG	2 cut(s)	2625, 6054
BshNI	GGYRCC	3 cut(s)	933, 4715, 4961
BshTI	ACCGGT	1 cut(s)	5342
BsiHKAI	GWGCWC	6 cut(s)	1128, 1525, 3760, 4695, 5901, 6807
BsiHKCI	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
BsiSI	CCGG	8 cut(s)	2330, 3904, 4649, 5208, 5293, 5343, 5374, 6569
BslFI	GGGAC	4 cut(s)	1738, 3920, 5523, 5963
BsmAI	GTCTC	7 cut(s)	977, 1521, 2084, 3513, 5120, 5666, 5747
BsmBI	CGTCTC	2 cut(s)	977, 5666
BsmFI	GGGAC	4 cut(s)	1738, 3920, 5523, 5963
BsmI	GAATGC	3 cut(s)	1464, 3769, 4889
Bso31I	GGTCTC	1 cut(s)	5120
BsoBI	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
Bsp119I	TTCGAA	1 cut(s)	5958
Bsp120I	GGGCCC	1 cut(s)	2339
Bsp1407I	TGTACA	4 cut(s)	370, 3886, 3924, 4423
Bsp19I	CCATGG	3 cut(s)	578, 2317, 3126
BspACI	CCGC	9 cut(s)	1248, 2394, 2524, 3250, 5305, 5522, 6054, 6353, 6661
BspFNI	CGCG	2 cut(s)	2625, 6054
BspHI	TCATGA	1 cut(s)	1468
BspMAI	CTGCAG	1 cut(s)	923
BspMI	ACCTGC	4 cut(s)	2209, 3701, 4424, 5788
BspOI	GCTAGC	1 cut(s)	2388
BspQI	GCTCTTC	1 cut(s)	5889
BspT104I	TTCGAA	1 cut(s)	5958
BspT107I	GGYRCC	3 cut(s)	933, 4715, 4961
BspTI	CTTAAG	1 cut(s)	3023
BspTNI	GGTCTC	1 cut(s)	5120
BsrBI	CCGCTC	1 cut(s)	5524
BsrDI	GCAATG	7 cut(s)	273, 717, 1764, 2250, 3075, 4055, 4107
BsrFI	RCCGGY	2 cut(s)	5207, 5342
BsrGI	TGTACA	4 cut(s)	370, 3886, 3924, 4423
BssAI	RCCGGY	2 cut(s)	5207, 5342
BssNAI	GTATAC	3 cut(s)	3894, 4471, 5383
BssNI	GRCGYC	1 cut(s)	934
BssSI	CACGAG	1 cut(s)	5595
BssT1I	CCWWGG	7 cut(s)	136, 578, 1700, 2317, 3126, 4212, 4620
Bst1107I	GTATAC	3 cut(s)	3894, 4471, 5383
Bst2BI	CACGAG	1 cut(s)	5595
Bst6I	CTCTTC	8 cut(s)	579, 2196, 2725, 2766, 4101, 5889, 6288, 6462
BstACI	GRCGYC	1 cut(s)	934
BstAFI	CTTAAG	1 cut(s)	3023
BstAPI	GCANNNNNTGC	3 cut(s)	470, 2452, 4130
BstAUI	TGTACA	4 cut(s)	370, 3886, 3924, 4423
BstBAI	YACGTR	5 cut(s)	76, 1949, 4178, 4280, 6305
BstBI	TTCGAA	1 cut(s)	5958
BstDSI	CCRYGG	3 cut(s)	578, 2317, 3126
BstEII	GGTNACC	1 cut(s)	701
BstFNI	CGCG	2 cut(s)	2625, 6054
BstH2I	RGCGCY	3 cut(s)	937, 3186, 6779
BstHHI	GCGC	4 cut(s)	936, 2625, 3185, 6778
BstMAI	GTCTC	7 cut(s)	977, 1521, 2084, 3513, 5120, 5666, 5747
BstNSI	RCATGY	8 cut(s)	19, 407, 596, 657, 1499, 1619, 4430, 5398
BstPI	GGTNACC	1 cut(s)	701
BstSLI	GKGCMC	6 cut(s)	51, 2343, 4727, 4966, 5426, 6265
BstSNI	TACGTA	1 cut(s)	76
BstUI	CGCG	2 cut(s)	2625, 6054
BstXI	CCANNNNNNTGG	2 cut(s)	1431, 5516
BstZ17I	GTATAC	3 cut(s)	3894, 4471, 5383
Bsu36I	CCTNAGG	2 cut(s)	5823, 5999
BsuI	GTATCC	2 cut(s)	3124, 4441
BtgI	CCRYGG	3 cut(s)	578, 2317, 3126
BtrI	CACGTC	1 cut(s)	4849
BtsI	GCAGTG	3 cut(s)	928, 3323, 6262
BveI	ACCTGC	4 cut(s)	2209, 3701, 4424, 5788
CaiI	CAGNNNCTG	4 cut(s)	874, 1052, 1136, 4907
CciI	TCATGA	1 cut(s)	1468
CfoI	GCGC	4 cut(s)	936, 2625, 3185, 6778
Cfr10I	RCCGGY	2 cut(s)	5207, 5342
Cfr9I	CCCGGG	1 cut(s)	3903
CseI	GACGC	4 cut(s)	3260, 3812, 5683, 6440
CspAI	ACCGGT	1 cut(s)	5342
CspCI	CAANNNNNGTGG	8 cut(s)	1634, 1669, 5970, 6005, 6238, 6273, 6331, 6366
DinI	GGCGCC	1 cut(s)	935
DraI	TTTAAA	5 cut(s)	1414, 1735, 3297, 5002, 5619
DraIII	CACNNNGTG	1 cut(s)	1140
EaeI	YGGCCR	3 cut(s)	270, 4563, 5371
Eam1104I	CTCTTC	8 cut(s)	579, 2196, 2725, 2766, 4101, 5889, 6288, 6462
EarI	CTCTTC	8 cut(s)	579, 2196, 2725, 2766, 4101, 5889, 6288, 6462
EciI	GGCGGA	1 cut(s)	1263
Ecl136II	GAGCTC	4 cut(s)	1523, 3758, 4693, 5899
Eco105I	TACGTA	1 cut(s)	76
Eco130I	CCWWGG	7 cut(s)	136, 578, 1700, 2317, 3126, 4212, 4620
Eco147I	AGGCCT	2 cut(s)	1696, 5560
Eco24I	GRGCYC	7 cut(s)	1525, 2343, 2677, 3760, 4695, 4761, 5901
Eco31I	GGTCTC	1 cut(s)	5120
Eco47I	GGWCC	9 cut(s)	757, 959, 1886, 3907, 4086, 4874, 5408, 5950, 6664
Eco47III	AGCGCT	2 cut(s)	3184, 6777
Eco53kI	GAGCTC	4 cut(s)	1523, 3758, 4693, 5899
Eco72I	CACGTG	2 cut(s)	4280, 6305
Eco81I	CCTNAGG	2 cut(s)	5823, 5999
Eco88I	CYCGRG	4 cut(s)	1518, 2745, 3903, 4805
Eco91I	GGTNACC	1 cut(s)	701
EcoICRI	GAGCTC	4 cut(s)	1523, 3758, 4693, 5899
EcoO109I	RGGNCCY	7 cut(s)	1886, 2339, 2340, 4086, 4492, 5408, 5950
EcoO65I	GGTNACC	1 cut(s)	701
EcoRI	GAATTC	3 cut(s)	122, 1472, 5581
EcoT14I	CCWWGG	7 cut(s)	136, 578, 1700, 2317, 3126, 4212, 4620
EcoT22I	ATGCAT	5 cut(s)	466, 3576, 4294, 4314, 4891
EcoT38I	GRGCYC	7 cut(s)	1525, 2343, 2677, 3760, 4695, 4761, 5901
EgeI	GGCGCC	1 cut(s)	935
EheI	GGCGCC	1 cut(s)	935
ErhI	CCWWGG	7 cut(s)	136, 578, 1700, 2317, 3126, 4212, 4620
Esp3I	CGTCTC	2 cut(s)	977, 5666
FalI	AAGNNNNNCTT	8 cut(s)	1684, 1716, 2670, 2702, 3192, 3224, 5952, 5984
FaqI	GGGAC	4 cut(s)	1738, 3920, 5523, 5963
FauI	CCCGC	3 cut(s)	2531, 3243, 6654
FauNDI	CATATG	3 cut(s)	2155, 4158, 5251
FbaI	TGATCA	6 cut(s)	880, 3223, 3232, 5323, 6115, 6396
FblI	GTMKAC	8 cut(s)	2943, 3700, 3893, 3936, 4470, 4799, 5382, 6327
FriOI	GRGCYC	7 cut(s)	1525, 2343, 2677, 3760, 4695, 4761, 5901
GlaI	GCGC	4 cut(s)	935, 2624, 3184, 6777
GsaI	CCCAGC	4 cut(s)	710, 1166, 5689, 5832
GsuI	CTGGAG	7 cut(s)	1476, 2229, 3679, 3851, 5535, 5944, 6209
HaeII	RGCGCY	3 cut(s)	937, 3186, 6779
HapII	CCGG	8 cut(s)	2330, 3904, 4649, 5208, 5293, 5343, 5374, 6569
HgaI	GACGC	4 cut(s)	3260, 3812, 5683, 6440
HhaI	GCGC	4 cut(s)	936, 2625, 3185, 6778
Hin1I	GRCGYC	1 cut(s)	934
Hin6I	GCGC	4 cut(s)	934, 2623, 3183, 6776
HinP1I	GCGC	4 cut(s)	934, 2623, 3183, 6776
HincII	GTYRAC	1 cut(s)	3035
HindII	GTYRAC	1 cut(s)	3035
HindIII	AAGCTT	3 cut(s)	3866, 5182, 5717
HpaII	CCGG	8 cut(s)	2330, 3904, 4649, 5208, 5293, 5343, 5374, 6569
Hpy99I	CGWCG	3 cut(s)	3276, 6456, 6602
Hsp92I	GRCGYC	1 cut(s)	934
HspAI	GCGC	4 cut(s)	934, 2623, 3183, 6776
KasI	GGCGCC	1 cut(s)	933
Ksp22I	TGATCA	6 cut(s)	880, 3223, 3232, 5323, 6115, 6396
LguI	GCTCTTC	1 cut(s)	5889
MbiI	CCGCTC	1 cut(s)	5524
MfeI	CAATTG	3 cut(s)	3411, 5202, 6683
MlsI	TGGCCA	2 cut(s)	272, 4565
MluNI	TGGCCA	2 cut(s)	272, 4565
Mly113I	GGCGCC	1 cut(s)	934
MmeI	TCCRAC	9 cut(s)	978, 1021, 1638, 1699, 1953, 4092, 5134, 6247, 6434
Mox20I	TGGCCA	2 cut(s)	272, 4565
Mph1103I	ATGCAT	5 cut(s)	466, 3576, 4294, 4314, 4891
MroXI	GAANNNNTTC	6 cut(s)	1270, 2832, 3088, 3528, 4743, 6282
MscI	TGGCCA	2 cut(s)	272, 4565
Msp20I	TGGCCA	2 cut(s)	272, 4565
MspA1I	CMGCKG	5 cut(s)	191, 2015, 6217, 6423, 6511
MspCI	CTTAAG	1 cut(s)	3023
MspI	CCGG	8 cut(s)	2330, 3904, 4649, 5208, 5293, 5343, 5374, 6569
MunI	CAATTG	3 cut(s)	3411, 5202, 6683
Mva1269I	GAATGC	3 cut(s)	1464, 3769, 4889
MvnI	CGCG	2 cut(s)	2625, 6054
NarI	GGCGCC	1 cut(s)	934
NciI	CCSGG	7 cut(s)	2330, 3904, 3905, 4650, 5293, 5375, 6570
NcoI	CCATGG	3 cut(s)	578, 2317, 3126
NdeI	CATATG	3 cut(s)	2155, 4158, 5251
NheI	GCTAGC	1 cut(s)	2384
NsiI	ATGCAT	5 cut(s)	466, 3576, 4294, 4314, 4891
NspI	RCATGY	8 cut(s)	19, 407, 596, 657, 1499, 1619, 4430, 5398
NspV	TTCGAA	1 cut(s)	5958
OliI	CACNNNNGTG	2 cut(s)	2892, 4400
PaeR7I	CTCGAG	1 cut(s)	1518
PagI	TCATGA	1 cut(s)	1468
PaqCI	CACCTGC	2 cut(s)	2209, 3701
PceI	AGGCCT	2 cut(s)	1696, 5560
PciI	ACATGT	4 cut(s)	403, 592, 1495, 4426
PciSI	GCTCTTC	1 cut(s)	5889
PcsI	WCGNNNNNNNCGW	1 cut(s)	4398
PctI	GAATGC	3 cut(s)	1464, 3769, 4889
PdmI	GAANNNNTTC	6 cut(s)	1270, 2832, 3088, 3528, 4743, 6282
PflFI	GACNNNGTC	1 cut(s)	220
PflMI	CCANNNNNTGG	2 cut(s)	584, 1370
PfoI	TCCNGGA	3 cut(s)	811, 1888, 6568
PinAI	ACCGGT	1 cut(s)	5342
PluTI	GGCGCC	1 cut(s)	937
PmaCI	CACGTG	2 cut(s)	4280, 6305
PmlI	CACGTG	2 cut(s)	4280, 6305
Ppu21I	YACGTR	5 cut(s)	76, 1949, 4178, 4280, 6305
PpuMI	RGGWCCY	4 cut(s)	1886, 4086, 5408, 5950
PscI	ACATGT	4 cut(s)	403, 592, 1495, 4426
PshBI	ATTAAT	2 cut(s)	545, 3486
PsiI	TTATAA	2 cut(s)	830, 3455
Psp124BI	GAGCTC	4 cut(s)	1525, 3760, 4695, 5901
Psp5II	RGGWCCY	4 cut(s)	1886, 4086, 5408, 5950
PspCI	CACGTG	2 cut(s)	4280, 6305
PspEI	GGTNACC	1 cut(s)	701
PspFI	CCCAGC	4 cut(s)	706, 1162, 5685, 5828
PspOMI	GGGCCC	1 cut(s)	2339
PspPPI	RGGWCCY	4 cut(s)	1886, 4086, 5408, 5950
PstI	CTGCAG	1 cut(s)	923
PstNI	CAGNNNCTG	4 cut(s)	874, 1052, 1136, 4907
PsyI	GACNNNGTC	1 cut(s)	220
PvuII	CAGCTG	5 cut(s)	191, 2015, 6217, 6423, 6511
SacI	GAGCTC	4 cut(s)	1525, 3760, 4695, 5901
SapI	GCTCTTC	1 cut(s)	5889
ScaI	AGTACT	2 cut(s)	1088, 5570
SfoI	GGCGCC	1 cut(s)	935
Sfr274I	CTCGAG	1 cut(s)	1518
SfuI	TTCGAA	1 cut(s)	5958
SinI	GGWCC	9 cut(s)	757, 959, 1886, 3907, 4086, 4874, 5408, 5950, 6664
SlaI	CTCGAG	1 cut(s)	1518
SmaI	CCCGGG	1 cut(s)	3905
SmlI	CTYRAG	7 cut(s)	1518, 1784, 2633, 3023, 3642, 6140, 6729
SmoI	CTYRAG	7 cut(s)	1518, 1784, 2633, 3023, 3642, 6140, 6729
SnaBI	TACGTA	1 cut(s)	76
SseBI	AGGCCT	2 cut(s)	1696, 5560
SsiI	CCGC	9 cut(s)	1248, 2394, 2524, 3250, 5305, 5522, 6054, 6353, 6661
SspDI	GGCGCC	1 cut(s)	933
SspI	AATATT	1 cut(s)	6694
SstI	GAGCTC	4 cut(s)	1525, 3760, 4695, 5901
StuI	AGGCCT	2 cut(s)	1696, 5560
StyI	CCWWGG	7 cut(s)	136, 578, 1700, 2317, 3126, 4212, 4620
TaqII	GACCGA	1 cut(s)	4563
TatI	WGTACW	7 cut(s)	370, 1086, 1396, 3886, 3924, 4423, 5568
TauI	GCSGC	1 cut(s)	5524
TspGWI	ACGGA	1 cut(s)	1992
TspMI	CCCGGG	1 cut(s)	3903
Tth111I	GACNNNGTC	1 cut(s)	220
Van91I	CCANNNNNTGG	2 cut(s)	584, 1370
Vha464I	CTTAAG	1 cut(s)	3023
VpaK11BI	GGWCC	9 cut(s)	757, 959, 1886, 3907, 4086, 4874, 5408, 5950, 6664
VspI	ATTAAT	2 cut(s)	545, 3486
XceI	RCATGY	8 cut(s)	19, 407, 596, 657, 1499, 1619, 4430, 5398
XcmI	CCANNNNNNNNNTGG	4 cut(s)	65, 1137, 1178, 4295
XhoI	CTCGAG	1 cut(s)	1518
XmaI	CCCGGG	1 cut(s)	3903
XmiI	GTMKAC	8 cut(s)	2943, 3700, 3893, 3936, 4470, 4799, 5382, 6327
XmnI	GAANNNNTTC	6 cut(s)	1270, 2832, 3088, 3528, 4743, 6282
ZrmI	AGTACT	2 cut(s)	1088, 5570
Zsp2I	ATGCAT	5 cut(s)	466, 3576, 4294, 4314, 4891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.