Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Forward (+)

67239813 .. 67252630

Gene Structure 12818 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG470600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6843 bp

ATGCAAGTATCGAAACGCCGCCCAAGTATAACCAGGATGTCAGAAGCTCAAAGGAGATTGTTGACAACTGTTAGTCTTCCTCGTAATAATAGTTACGTAAATGGGGGACTATCAATCAGGTCCCCTGCTGCAGCACCTGCAGTTGAGGAGTTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATAGCAAACAATGGAATGGCAGCAGTCAAGTTTATACGTAGTGTGAGGACGTGGGCTTATGAAACATTTGGTACAGAAAAGGCTGTTTTGTTGGTGGCCATGGCCACACCAGAAGACATGAGAATCAATGCAGAACACATTAGAATTGCTGATCAGTTTGTGGAAGTTCCTGGTGGAACTAACAATAATAACTATGCCAATGTGCAACTCATTGTAGAGATGGCAGAAATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCATCTGAGATCCCTGAGCTTCCAGATGCATTGACTGCAAAGGGCATCATATTTCTCGGTCCACCAGCTGTATCTATGGCAGCATTAGGAGATAAAATTGGTTCATCATTAATTGCTCAAGCTGCTGAAGTACCCACCCTTCCATGGAGTGGATCACATGTAAAAATTGCTTCAGACGGCTGCCTGGTTACGATCCCAGATGAAATATATCGGGAAGCATGTGTTTACACAACAGAGGAAGCGATTGCAAGTTGTCAGGTTGTTGGTTACCCAGCCATGATTAAGGCATCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAAGTTAGGGCATTGTTCAAGCAAGTCCAGGGTGAAGTTCCTGGATCTCCCATATTCATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAAGTCCAGTTACTATGTGATCAGCATGGAAATGTTGCAGCTTTGCACAGCCGTGATTGCAGTGTTCAAAGGAGGCACCAGAAGATTATTGAGGAGGGTCCAATAACTGTAGCTCCACCAGAGACAATTAAAAAGTTGGAGCAGGCGGCTCGAAGGTTGGCTAAAAGTGTTAACTATGTTGGAGCAGCTACTGTTGAGTATCTGTACAGTATGGACACTGGCGAGTACTATTTCTTAGAGTTGAACCCTCGGTTGCAGGTGGAGCACCCAGTCACCGAGTGGATAGCAGAAATAAATCTGCCAGCAGCCCAAGTTGCAGTTGGGATGGGCATTCCCCTATGGCAAATTCCTGAGATAAGGCGGTTCTATGGAATGGAACATGGTAGCGGATATGATGCTTGGAGAAAGACGTCAGTTGTTGCCACCCCATTTGACTTTGACAAGGCAGAATCTACAAGGCCAAAAGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAACCTACTAGCGGGAAAGTTCAGGAGCTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCGTACTTTTCTGTTAAGTCTGGAGGGGGGATTCATGAGTACTCAGATTCTCAATTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTGGATTATTCAATAGATCTTTTACATGCTTCAGATTACAGAGAAAATAAAATCCACACAGGTTGGTTGGATAGTAGAATTGCTATGCGAGTTAGAACAGAAAGGCCTCCTTGGTATTTGTCTGTAATTGGAGGGACTCTTTTTAAAGCATCTGCTAGTAGTGCGGCGATGGTTTCAGATTATATCGGCTATCTTGAGAAAGGGCAAATCCCACCTAAGCATATATCATTTGTCCATTCACAAGTGTCTTTGAACATTGAGGGGAGCAAATACACGATTGACATGGTGAGAGGGGGTCCTGGGAGCTATAGATTGCGGATGAATGAGTCAGAGGTTGAAGCAGAGATACATACTTTACGCGATGGAGGATTACTGATGCAGTTGGATGGAAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGTCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGATCACGATCCATCCAAGTTAATAGCAGAAACACCATGCAAGCTGTTGAGGTTCTTGGTTGCTGATGATAGTCATATCGATGCTGATACACCATATGCAGAGGTTGAGGTTATGAAGATGTGTATGCCTCTACTTTCACCTGCTTCTGGAGTTATCCACTTTCAATTGTCTGAAGGCCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCAGCTGTGAGAAAGACAGAACCTTTCCGTGGAAGCTTCCCTGTCCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTTCATCAAAGATGTGCTGCAAGCTTAAACGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCCATTGCCTAGATAGTCCTGAACTCCCTTTCCTTCAATGGCAAGAATGCTTGGCTGTTTTGGCAACCCGCCTACCCAAGAATCTCAAAAATGAACTGGAATCAAAGTTTAAGGATTTTGAGTTGATTTCTAGCTCTCAGAATGTTGATTTCCCTGCCAAATTGTTGCGTGGTGTTCTTGAGGCTCATCTATTCTCCTGTCCTGATAAAGAAAAAGGAGCCCAAGAAAGGCTTGTTGAACCTTTGATGAGCCTTGTGACGTCTTATGAAGGTGGAAGAGAAAGTCATGCCCGAGTTATTGTTCAATCCCTCTTTGAAGAGTATCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAGCGCCTTCGACTTCAATATAAGAAAGATCTGTTGAAGGTTGTGGAAATTGTGCTCTCTCATCAGGGTGTAAAGAATAAAAATAAGTTGATTCTACGACTCATGGAACAACTGGTGTATCCCAACCCTGCTGCATACAGGGAGAAGCTAATTCGGTTTTCTCAACTTAACCATACAAACTATTCTCAGTTGGCACTAAAAGCAAGTCAGCTTATGGAGCAAACCAAATTGAGTGAACTTCGCTCTAGCATTGCTAGAAGTCTTTCTGAGTTGGAGATGTTTACTGAGGATGGTGAAACCATGGATACTCCAAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCACCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTCTTTGATCATGGTGATCACACCCTTCAGAGGCGGGTTGTCGAGAGTTATGTACGCCGGTTATACCAGCCCTACCTTGTAAAGGGGAGTGTCAGGATGCAGTGGCACAGATCTGGTCTAATAGCTTCATGGGAGTTCTCGGAAGAACCTGTTGAGAGGAAACATGAGATTGAAGGTCAATCAGTTGAGAAAACATTCCTTCAGAAACTCGAGGAGAGGAAATGGGGAGTCATGGTTATAATAAAATCTCTTCAGTTTCTTCCTGCAATTATTAGTGGCGCACTGAAGGAAATGTCTCATAACCTTCATGAAGCAACTCCAAATGGATCTACTGAACCAAGTGGCTTTGGTAATATGATGCATATTGCTTTGGTTGGCATCAACAATCAGATGAGTCTACTTCAGGATAGTGGTGACGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATACTTAAAGAGCAAGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCACAGTAATTAGTTGTATCATACAAAGGGATGAAGGGCGGGCCCCTATGAGGCACTCCTTCCACTGGTCATCAGAGAAGCTCTATTATGAAGAAGAACCCCTATTGCGTCACCTAGAACCTCCTCTATCTATTTACCTTGAATTGGACAAGCTTAAAGGCTATGAGAATATACAGTATACCCCATCTCGTGACCGTCAATGGCACTTGTACACCGTTGTAGACAAGCCACTGCCAATCCAAAGGATGTTCCTCAGAACACTTGTCAGGCAGCCCACAACAAATGAAGGGTTCTCTGGATTTCAACGGCTAGACATTGCAACAGCTAGTAACCAATTTTCTTTATCATTTACTTCGAGGAGCATCTTGAGGTCCTTATCAACAGCAATGGAAGAGTTGGAACTGAATGCGCACAATGCCACTGTCAAATCTGACCACACCCATATGTATCTTTATATCTTACGTGAGCAACAAATTAATGATCTGCTGCCTTACCACAAGAGAGTTGATTTAGATGCTCAACAAGAAGAAACTGTTGTGGAGGCCATCTTGGAAGAACTTGCACGTGAAATCCACACATCAGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGCTGTGGATGGCATCCTCTGGAGAGGCAAACGTTGCTTGGAGGGTTGTCGTGACAAATGTGACTGGTCATACCTGCACTGTACATATATATCGGGAACTAGAAGGTACCAGCAAACAGGGAGCGGTATACCATTCAGTCTCTGTACAGGGTCCCCTGCATGGTGTACCAGTGAATGCGCACTATCAGCCTTTGGGAATTATAGACCGAAAACGTCTGTTGGCCAGGAGAACCAACACCACTTACTGCTATGATTTTCCACTGGCATTTGAGACGGCCTTGGAACAGTCATGGGCATCCCAGTCACCAGGTGTTGACAAACCGAAGGGTAAAATTCTCAAGGTCACAGAGCTCAAATTCGCTGACCAAAAAGGCAGCTGGGGTACTCCTCTCAATACCGTAGAGCGTCCACCTGGACTCAATGATGTTGGTATGATAGCCTGGTTTATGGAGATGTCTACCCCTGAGTTCCCTTCTGGAAGGAGGATATTGGTTGTTGCGAATGATGTCACCTACAAAGCTGGATCTTTTGGCCCAAGGGAGGATGCATTCTTCTTTGCAGTAACTGAGCTTGCTTGTGCTGAGAAATTGCCTCTAATATACTTGGCAGCAAACTCAGGTGCCCGTATTGGGGTTGCTGAAGAAGTCAAATCCTGTTTTAAAGTGGGCTGGTCTGATGAATCAAGCCCCGAACGTGGTTTTCAATATGTGTATTTAACCTCTAAGGATTATGCTCGGATTAAATCATCTGTGATAGCGCATGAATTAAACCTAGCAAATGGCGAAAGCAGATGGGTTATAGATACCATTGTTGGAAAGGAGGACGGCTTGGGGGTCGAGAGCTTGACTGGAAGTGGGGCAATTGCTGGTGCATATTCGAGGGCATATATGGAAACCTTTACCTTAACATATGTGACGGGCAGAACTGTAGGGATAGGTGCTTATCTTGCAAGGCTTGGGATGCGGTGCATCCAGAGGCTTGATCAGCCCATCATTTTAACTGGTTTCTCTGCGTTAAACAAACTACTTGGCCGGGAGGTTTACAGCTCCCATATGCAACTTGGAGGACCTAAAATTATGGGAACTAATGGGGTTGTACATCTAACGGTAGCTGATGATCTTGAAGGAATATCTGCTATTCTAAAGTGGCTGAGCTATGTTCCTCCCCATGTTGGTGGTCCACTCCCCATTTCAATTCCCTTGGATCCTCCAGAAAGGCGTGTGGAGTACTGCCCCGAGAATTCATGTGATCCTCGTGCTGCGATTTCTGGTGCTCTGAATGGTAATGGGAACTGGATGGGGGGTATATTTGACAAAGACAGCTTTGTTGAGACGCTAGAAGGCTGGGCAAGGACGGTTGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTGGGAATTGTTGCTGTTGAGACACAGACGGTGATGCAAATTATTCCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGACAAGTATGGTTTCCTGATTCTGCAACTAAGACTACCCAAGCATTATTAGATTTCAACAGAGAAGGACTCCCACTTTTCATTCTTGCCAACTGGAGAGGCTTTTCTGGTGGGCAGAGAGACCTTTTCGAAGGGATCCTTCAGGCTGGATCAACCATTGTGGAGAACCTAAGGACTTACAAACAGCCCATTTTTGTATTCATCCCAATGATGGGTGAGCTCCGCGGTGGAGCATGGGTGGTCGTTGATAGCCGGATAAATCCGGACCATATTGAAATGTATGCTGATCGAACAGCTAGAGGAAATGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTTAGGAACAAGGAATTGCTGGAGTGCATGGGCAGGCTTGACCAACAGCTGATCCAACTAAAGACTAAGCTTCAGGAAGCCAAGATCTGTGGGGCGAATGAGAAGGTTGAATCCCTACAGTTTCAGATACAATCCCGAGAAAAACAGCTTTTGCCCGTCTACACTCAGATAGCTACCAAATTTGCAGAACTTCATGATACGTCTCTGAGGATGGCTGCAAAAGGGGTAATCAGAGGAGTTTTGGAGTGGGCCAGCTCTCGATCTTTCTTCTACAAGAGATTGCGTCGGAGAATTGCCGATGACTCGCTCATCAAGACAGTGATAGATGCTGCTGGTGAACAGCTGTCACATAGATCTGCAATGGATTTGATCAAGAACTGGTTTTTGAGTTCTGATATTTCAAGAGGCAGGGAAAATGCTTGGGAGGATGATGAAACTTTCTTTAGATGGAAGGATGATCAAACTAATTATGAGGGTAAGCTAAAAGAATTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAACCATTGGCAACTCAGCTTCTGATTTGCAAGCCCTACCTCAAGGTCTTGCTGCCCTTCTAAGCAAGGTTGAGCCATCAAGCAGATCATTGTTGGTTGAGGAACTCCGGAAGGTGCTTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2280

Amino Acids

254.23

Weight (kDa)

6.13

Isoelectric Point (pI)

42.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	61 - 180	5.1e-28	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	228 - 410	5.4e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	229 - 380	2.8e-07	ATP-grasp domain
ATP-grasp_3	PF02655	235 - 382	2.4e-07	ATP-grasp domain
Biotin_carb_C	PF02785	457 - 563	1.1e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	579 - 694	3.9e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	705 - 767	1e-08	Biotin-requiring enzyme
ACC_central	PF08326	768 - 1511	1.1e-176	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1613 - 2164	6.5e-149	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3482
AarI	CACCTGC	4 cut(s)	145, 1135, 2236, 3731
AatII	GACGTC	2 cut(s)	1301, 2744
Acc16I	TGCGCA	2 cut(s)	4155, 4551
Acc36I	ACCTGC	5 cut(s)	145, 1135, 2236, 3731, 4454
Acc65I	GGTACC	1 cut(s)	4478
AccB1I	GGYRCC	3 cut(s)	963, 4478, 4991
AccB7I	CCANNNNNTGG	2 cut(s)	611, 1397
AccBSI	CCGCTC	1 cut(s)	4496
AccI	GTMKAC	7 cut(s)	3640, 3730, 3923, 3966, 4500, 4829, 6357
AccII	CGCG	2 cut(s)	1977, 6084
AccIII	TCCGGA	2 cut(s)	6121, 6825
AclI	AACGTT	1 cut(s)	4404
AcoI	YGGCCR	4 cut(s)	291, 297, 4593, 5401
AcsI	RAATTY	8 cut(s)	1233, 1587, 1593, 1605, 4704, 4727, 5611, 6377
AcvI	CACGTG	1 cut(s)	4310
AcyI	GRCGYC	2 cut(s)	1298, 2741
AdeI	CACNNNGTG	2 cut(s)	1167, 4682
AflIII	ACRYGT	3 cut(s)	430, 619, 1522
AjiI	CACGTC	1 cut(s)	246
AjuI	GAANNNNNNNTTGG	2 cut(s)	2365, 2397
AleI	CACNNNNGTG	1 cut(s)	939
AloI	GAACNNNNNNTCC	2 cut(s)	2496, 2528
Alw21I	GWGCWC	7 cut(s)	1155, 1552, 2910, 3214, 4725, 5647, 6081
Alw44I	GTGCAC	1 cut(s)	3210
AlwNI	CAGNNNCTG	6 cut(s)	137, 1079, 1432, 4514, 4937, 6740
Ama87I	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
Aor13HI	TCCGGA	2 cut(s)	6121, 6825
ApaI	GGGCCC	1 cut(s)	3790
ApaLI	GTGCAC	1 cut(s)	3210
ApoI	RAATTY	8 cut(s)	1233, 1587, 1593, 1605, 4704, 4727, 5611, 6377
AseI	ATTAAT	2 cut(s)	572, 4221
Asp700I	GAANNNNTTC	2 cut(s)	3115, 3437
Asp718I	GGTACC	1 cut(s)	4478
AspLEI	GCGC	5 cut(s)	2859, 3524, 4156, 4552, 5131
AsuC2I	CCSGG	1 cut(s)	5405
AsuII	TTCGAA	1 cut(s)	5988
AvaI	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
AxyI	CCTNAGG	2 cut(s)	5853, 6029
BaeGI	GKGCMC	3 cut(s)	3214, 3790, 4996
BaeI	ACNNNNGTAYC	4 cut(s)	2954, 2987, 5166, 5199
BalI	TGGCCA	3 cut(s)	293, 299, 4595
BamHI	GGATCC	3 cut(s)	1387, 5575, 5994
BanI	GGYRCC	3 cut(s)	963, 4478, 4991
BanII	GRGCYC	5 cut(s)	1552, 2704, 3790, 4725, 6081
BarI	GAAGNNNNNNTAC	6 cut(s)	585, 617, 882, 914, 1947, 1979
BauI	CACGAG	2 cut(s)	3933, 5625
BbrPI	CACGTG	1 cut(s)	4310
BbsI	GAAGAC	3 cut(s)	68, 315, 2045
Bbv12I	GWGCWC	7 cut(s)	1155, 1552, 2910, 3214, 4725, 5647, 6081
BceAI	ACGGC	5 cut(s)	655, 924, 4067, 4662, 5212
BcgI	CGANNNNNNTGC	2 cut(s)	1019, 1053
BciVI	GTATCC	2 cut(s)	2982, 3151
BclI	TGATCA	7 cut(s)	346, 907, 3250, 3259, 5353, 6567, 6655
BcnI	CCSGG	1 cut(s)	5405
BfmI	CTRYAG	6 cut(s)	129, 138, 996, 1924, 5298, 6314
BfoI	RGCGCY	1 cut(s)	2860
BfuAI	ACCTGC	5 cut(s)	145, 1135, 2236, 3731, 4454
BfuI	GTATCC	2 cut(s)	2982, 3151
BglII	AGATCT	5 cut(s)	1633, 2881, 3353, 6282, 6551
BlpI	GCTNAGC	1 cut(s)	5522
BmcAI	AGTACT	3 cut(s)	1115, 1502, 5600
BmeT110I	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
BmgBI	CACGTC	1 cut(s)	246
BmrI	ACTGGG	2 cut(s)	1151, 4666
BmuI	ACTGGG	2 cut(s)	1151, 4666
BpiI	GAAGAC	3 cut(s)	68, 315, 2045
BplI	GAGNNNNNCTC	2 cut(s)	5913, 5945
BpmI	CTGGAG	7 cut(s)	1503, 2256, 3709, 4413, 5565, 5974, 6239
Bpu10I	CCTNAGC	2 cut(s)	477, 1833
Bpu1102I	GCTNAGC	1 cut(s)	5522
Bpu14I	TTCGAA	1 cut(s)	5988
BpuEI	CTTGAG	8 cut(s)	564, 1832, 2681, 3657, 4132, 4694, 6191, 6744
BpuMI	CCSGG	1 cut(s)	5405
Bsa29I	ATCGAT	1 cut(s)	2166
BsaAI	YACGTR	4 cut(s)	97, 233, 4208, 4310
BsaBI	GATNNNNATC	2 cut(s)	2094, 6191
BsaHI	GRCGYC	2 cut(s)	1298, 2741
BsaI	GGTCTC	2 cut(s)	3248, 5973
BsaWI	WCCGGW	2 cut(s)	6121, 6825
BsaXI	ACNNNNNCTCC	6 cut(s)	985, 1015, 3462, 3492, 6081, 6111
Bse118I	RCCGGY	1 cut(s)	3300
Bse21I	CCTNAGG	2 cut(s)	5853, 6029
Bse3DI	GCAATG	6 cut(s)	2277, 2485, 3102, 4059, 4137, 6564
Bse8I	GATNNNNATC	2 cut(s)	2094, 6191
BseAI	TCCGGA	2 cut(s)	6121, 6825
BseCI	ATCGAT	1 cut(s)	2166
BseJI	GATNNNNATC	2 cut(s)	2094, 6191
BseMI	GCAATG	6 cut(s)	2277, 2485, 3102, 4059, 4137, 6564
BseRI	GAGGAG	8 cut(s)	161, 995, 1614, 3470, 3858, 4117, 4749, 6447
BseSI	GKGCMC	3 cut(s)	3214, 3790, 4996
BseYI	CCCAGC	3 cut(s)	733, 4749, 5715
BsgI	GTGCAG	2 cut(s)	3759, 4432
Bsh1236I	CGCG	2 cut(s)	1977, 6084
BshNI	GGYRCC	3 cut(s)	963, 4478, 4991
BshVI	ATCGAT	1 cut(s)	2166
BsiHKAI	GWGCWC	7 cut(s)	1155, 1552, 2910, 3214, 4725, 5647, 6081
BsiHKCI	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
BsiSI	CCGG	5 cut(s)	3301, 5404, 6112, 6122, 6826
BslFI	GGGAC	4 cut(s)	106, 120, 1765, 4509
BsmBI	CGTCTC	3 cut(s)	4637, 5696, 6405
BsmFI	GGGAC	4 cut(s)	106, 120, 1765, 4509
BsmI	GAATGC	5 cut(s)	1218, 2534, 4156, 4552, 4919
Bso31I	GGTCTC	2 cut(s)	3248, 5973
BsoBI	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
Bsp119I	TTCGAA	1 cut(s)	5988
Bsp120I	GGGCCC	1 cut(s)	3786
Bsp13I	TCCGGA	2 cut(s)	6121, 6825
Bsp1407I	TGTACA	5 cut(s)	1092, 3954, 4453, 4516, 5467
Bsp1720I	GCTNAGC	1 cut(s)	5522
Bsp19I	CCATGG	3 cut(s)	294, 605, 3153
BspDI	ATCGAT	1 cut(s)	2166
BspEI	TCCGGA	2 cut(s)	6121, 6825
BspFNI	CGCG	2 cut(s)	1977, 6084
BspHI	TCATGA	3 cut(s)	1495, 3550, 6391
BspMAI	CTGCAG	2 cut(s)	133, 142
BspMI	ACCTGC	5 cut(s)	145, 1135, 2236, 3731, 4454
BspT104I	TTCGAA	1 cut(s)	5988
BspT107I	GGYRCC	3 cut(s)	963, 4478, 4991
BspTNI	GGTCTC	2 cut(s)	3248, 5973
BsrBI	CCGCTC	1 cut(s)	4496
BsrDI	GCAATG	6 cut(s)	2277, 2485, 3102, 4059, 4137, 6564
BsrFI	RCCGGY	1 cut(s)	3300
BsrGI	TGTACA	5 cut(s)	1092, 3954, 4453, 4516, 5467
BssAI	RCCGGY	1 cut(s)	3300
BssNAI	GTATAC	2 cut(s)	3924, 4501
BssNI	GRCGYC	2 cut(s)	1298, 2741
BssSI	CACGAG	2 cut(s)	3933, 5625
BssT1I	CCWWGG	8 cut(s)	163, 294, 605, 1727, 3153, 4650, 4907, 5571
Bst1107I	GTATAC	2 cut(s)	3924, 4501
Bst2BI	CACGAG	2 cut(s)	3933, 5625
Bst6I	CTCTTC	4 cut(s)	2752, 2793, 3498, 4131
BstACI	GRCGYC	2 cut(s)	1298, 2741
BstAPI	GCANNNNNTGC	4 cut(s)	137, 497, 2479, 4406
BstAUI	TGTACA	5 cut(s)	1092, 3954, 4453, 4516, 5467
BstBAI	YACGTR	4 cut(s)	97, 233, 4208, 4310
BstBI	TTCGAA	1 cut(s)	5988
BstDSI	CCRYGG	5 cut(s)	294, 605, 2344, 3153, 6082
BstEII	GGTNACC	1 cut(s)	728
BstENI	CCTNNNNNAGG	1 cut(s)	3323
BstFNI	CGCG	2 cut(s)	1977, 6084
BstH2I	RGCGCY	1 cut(s)	2860
BstHHI	GCGC	5 cut(s)	2859, 3524, 4156, 4552, 5131
BstNSI	RCATGY	5 cut(s)	434, 623, 684, 1526, 1646
BstPI	GGTNACC	1 cut(s)	728
BstSFI	CTRYAG	6 cut(s)	129, 138, 996, 1924, 5298, 6314
BstSLI	GKGCMC	3 cut(s)	3214, 3790, 4996
BstSNI	TACGTA	2 cut(s)	97, 233
BstUI	CGCG	2 cut(s)	1977, 6084
BstV2I	GAAGAC	3 cut(s)	68, 315, 2045
BstXI	CCANNNNNNTGG	2 cut(s)	1458, 5546
BstZ17I	GTATAC	2 cut(s)	3924, 4501
Bsu15I	ATCGAT	1 cut(s)	2166
Bsu36I	CCTNAGG	2 cut(s)	5853, 6029
BsuI	GTATCC	2 cut(s)	2982, 3151
BsuTUI	ATCGAT	1 cut(s)	2166
BtgI	CCRYGG	5 cut(s)	294, 605, 2344, 3153, 6082
BtgZI	GCGATG	2 cut(s)	1799, 1992
BtrI	CACGTC	1 cut(s)	246
BtsI	GCAGTG	3 cut(s)	955, 3350, 3974
BveI	ACCTGC	5 cut(s)	145, 1135, 2236, 3731, 4454
CaiI	CAGNNNCTG	6 cut(s)	137, 1079, 1432, 4514, 4937, 6740
CciI	TCATGA	3 cut(s)	1495, 3550, 6391
CfoI	GCGC	5 cut(s)	2859, 3524, 4156, 4552, 5131
Cfr10I	RCCGGY	1 cut(s)	3300
Cfr42I	CCGCGG	1 cut(s)	6085
ClaI	ATCGAT	1 cut(s)	2166
CseI	GACGC	4 cut(s)	3842, 4766, 5713, 6470
CsiI	ACCWGGT	1 cut(s)	4678
CspCI	CAANNNNNGTGG	8 cut(s)	1300, 1335, 1661, 1696, 6000, 6035, 6268, 6303
DraI	TTTAAA	4 cut(s)	1405, 1441, 1762, 5032
DraIII	CACNNNGTG	2 cut(s)	1167, 4682
EaeI	YGGCCR	4 cut(s)	291, 297, 4593, 5401
Eam1104I	CTCTTC	4 cut(s)	2752, 2793, 3498, 4131
EarI	CTCTTC	4 cut(s)	2752, 2793, 3498, 4131
Ecl136II	GAGCTC	3 cut(s)	1550, 4723, 6079
Eco105I	TACGTA	2 cut(s)	97, 233
Eco130I	CCWWGG	8 cut(s)	163, 294, 605, 1727, 3153, 4650, 4907, 5571
Eco147I	AGGCCT	1 cut(s)	1723
Eco24I	GRGCYC	5 cut(s)	1552, 2704, 3790, 4725, 6081
Eco31I	GGTCTC	2 cut(s)	3248, 5973
Eco53kI	GAGCTC	3 cut(s)	1550, 4723, 6079
Eco72I	CACGTG	1 cut(s)	4310
Eco81I	CCTNAGG	2 cut(s)	5853, 6029
Eco88I	CYCGRG	5 cut(s)	1545, 2772, 3452, 5606, 6333
Eco91I	GGTNACC	1 cut(s)	728
EcoICRI	GAGCTC	3 cut(s)	1550, 4723, 6079
EcoNI	CCTNNNNNAGG	1 cut(s)	3323
EcoO109I	RGGNCCY	7 cut(s)	120, 1913, 2366, 3787, 4116, 4523, 5438
EcoO65I	GGTNACC	1 cut(s)	728
EcoRI	GAATTC	1 cut(s)	5611
EcoT14I	CCWWGG	8 cut(s)	163, 294, 605, 1727, 3153, 4650, 4907, 5571
EcoT22I	ATGCAT	5 cut(s)	466, 493, 3606, 4344, 4921
EcoT38I	GRGCYC	5 cut(s)	1552, 2704, 3790, 4725, 6081
ErhI	CCWWGG	8 cut(s)	163, 294, 605, 1727, 3153, 4650, 4907, 5571
Esp3I	CGTCTC	3 cut(s)	4637, 5696, 6405
FalI	AAGNNNNNCTT	8 cut(s)	1711, 1743, 2697, 2729, 3219, 3251, 5982, 6014
FaqI	GGGAC	4 cut(s)	106, 120, 1765, 4509
FauI	CCCGC	5 cut(s)	1409, 2558, 3270, 3777, 6684
FauNDI	CATATG	4 cut(s)	2182, 4188, 5281, 5424
FbaI	TGATCA	7 cut(s)	346, 907, 3250, 3259, 5353, 6567, 6655
FblI	GTMKAC	7 cut(s)	3640, 3730, 3923, 3966, 4500, 4829, 6357
FriOI	GRGCYC	5 cut(s)	1552, 2704, 3790, 4725, 6081
FspAI	RTGCGCAY	2 cut(s)	4155, 4551
FspI	TGCGCA	2 cut(s)	4155, 4551
GlaI	GCGC	5 cut(s)	2858, 3523, 4155, 4551, 5130
GsaI	CCCAGC	3 cut(s)	737, 4753, 5719
GsuI	CTGGAG	7 cut(s)	1503, 2256, 3709, 4413, 5565, 5974, 6239
HaeII	RGCGCY	1 cut(s)	2860
HapII	CCGG	5 cut(s)	3301, 5404, 6112, 6122, 6826
HgaI	GACGC	4 cut(s)	3842, 4766, 5713, 6470
HhaI	GCGC	5 cut(s)	2859, 3524, 4156, 4552, 5131
Hin1I	GRCGYC	2 cut(s)	1298, 2741
Hin6I	GCGC	5 cut(s)	2857, 3522, 4154, 4550, 5129
HinP1I	GCGC	5 cut(s)	2857, 3522, 4154, 4550, 5129
HincII	GTYRAC	3 cut(s)	63, 1060, 4687
HindII	GTYRAC	3 cut(s)	63, 1060, 4687
HindIII	AAGCTT	5 cut(s)	2350, 2413, 3896, 5747, 6266
HpaI	GTTAAC	1 cut(s)	1060
HpaII	CCGG	5 cut(s)	3301, 5404, 6112, 6122, 6826
Hpy99I	CGWCG	1 cut(s)	6486
Hsp92I	GRCGYC	2 cut(s)	1298, 2741
HspAI	GCGC	5 cut(s)	2857, 3522, 4154, 4550, 5129
KflI	GGGWCCC	1 cut(s)	4523
Kpn2I	TCCGGA	2 cut(s)	6121, 6825
KpnI	GGTACC	1 cut(s)	4482
Ksp22I	TGATCA	7 cut(s)	346, 907, 3250, 3259, 5353, 6567, 6655
KspAI	GTTAAC	1 cut(s)	1060
KspI	CCGCGG	1 cut(s)	6085
MabI	ACCWGGT	1 cut(s)	4678
MbiI	CCGCTC	1 cut(s)	4496
MfeI	CAATTG	3 cut(s)	2252, 5232, 6713
MlsI	TGGCCA	3 cut(s)	293, 299, 4595
MluNI	TGGCCA	3 cut(s)	293, 299, 4595
MlyI	GAGTC	9 cut(s)	1550, 1747, 1952, 2946, 3480, 3646, 4782, 5922, 6494
Mox20I	TGGCCA	3 cut(s)	293, 299, 4595
Mph1103I	ATGCAT	5 cut(s)	466, 493, 3606, 4344, 4921
MroI	TCCGGA	2 cut(s)	6121, 6825
MroXI	GAANNNNTTC	2 cut(s)	3115, 3437
MscI	TGGCCA	3 cut(s)	293, 299, 4595
MslI	CAYNNNNRTG	9 cut(s)	435, 792, 918, 939, 2166, 2919, 4187, 5544, 6065
Msp20I	TGGCCA	3 cut(s)	293, 299, 4595
MspA1I	CMGCKG	7 cut(s)	530, 2042, 2321, 4749, 6084, 6247, 6541
MspI	CCGG	5 cut(s)	3301, 5404, 6112, 6122, 6826
MunI	CAATTG	3 cut(s)	2252, 5232, 6713
Mva1269I	GAATGC	5 cut(s)	1218, 2534, 4156, 4552, 4919
MvnI	CGCG	2 cut(s)	1977, 6084
NciI	CCSGG	1 cut(s)	5405
NcoI	CCATGG	3 cut(s)	294, 605, 3153
NdeI	CATATG	4 cut(s)	2182, 4188, 5281, 5424
NsbI	TGCGCA	2 cut(s)	4155, 4551
NsiI	ATGCAT	5 cut(s)	466, 493, 3606, 4344, 4921
NspI	RCATGY	5 cut(s)	434, 623, 684, 1526, 1646
NspV	TTCGAA	1 cut(s)	5988
OliI	CACNNNNGTG	1 cut(s)	939
PaeR7I	CTCGAG	2 cut(s)	1545, 3452
PagI	TCATGA	3 cut(s)	1495, 3550, 6391
PaqCI	CACCTGC	4 cut(s)	145, 1135, 2236, 3731
PceI	AGGCCT	1 cut(s)	1723
PciI	ACATGT	3 cut(s)	430, 619, 1522
PctI	GAATGC	5 cut(s)	1218, 2534, 4156, 4552, 4919
PdmI	GAANNNNTTC	2 cut(s)	3115, 3437
PflMI	CCANNNNNTGG	2 cut(s)	611, 1397
PfoI	TCCNGGA	1 cut(s)	838
PleI	GAGTC	9 cut(s)	1549, 1747, 1951, 2946, 3479, 3645, 4782, 5922, 6494
PmaCI	CACGTG	1 cut(s)	4310
PmlI	CACGTG	1 cut(s)	4310
PpsI	GAGTC	9 cut(s)	1549, 1747, 1951, 2946, 3479, 3645, 4782, 5922, 6494
Ppu21I	YACGTR	4 cut(s)	97, 233, 4208, 4310
PpuMI	RGGWCCY	5 cut(s)	120, 1913, 4116, 4523, 5438
PscI	ACATGT	3 cut(s)	430, 619, 1522
PshBI	ATTAAT	2 cut(s)	572, 4221
PsiI	TTATAA	1 cut(s)	3482
Psp124BI	GAGCTC	3 cut(s)	1552, 4725, 6081
Psp1406I	AACGTT	1 cut(s)	4404
Psp5II	RGGWCCY	5 cut(s)	120, 1913, 4116, 4523, 5438
PspCI	CACGTG	1 cut(s)	4310
PspEI	GGTNACC	1 cut(s)	728
PspFI	CCCAGC	3 cut(s)	733, 4749, 5715
PspOMI	GGGCCC	1 cut(s)	3786
PspPPI	RGGWCCY	5 cut(s)	120, 1913, 4116, 4523, 5438
PspXI	VCTCGAGB	1 cut(s)	3452
PstI	CTGCAG	2 cut(s)	133, 142
PstNI	CAGNNNCTG	6 cut(s)	137, 1079, 1432, 4514, 4937, 6740
PvuII	CAGCTG	6 cut(s)	530, 2042, 2321, 4749, 6247, 6541
RseI	CAYNNNNRTG	9 cut(s)	435, 792, 918, 939, 2166, 2919, 4187, 5544, 6065
SacI	GAGCTC	3 cut(s)	1552, 4725, 6081
SacII	CCGCGG	1 cut(s)	6085
ScaI	AGTACT	3 cut(s)	1115, 1502, 5600
SchI	GAGTC	9 cut(s)	1550, 1747, 1952, 2946, 3480, 3646, 4782, 5922, 6494
SexAI	ACCWGGT	1 cut(s)	4678
SfcI	CTRYAG	6 cut(s)	129, 138, 996, 1924, 5298, 6314
Sfr274I	CTCGAG	2 cut(s)	1545, 3452
Sfr303I	CCGCGG	1 cut(s)	6085
SfuI	TTCGAA	1 cut(s)	5988
SgrBI	CCGCGG	1 cut(s)	6085
SlaI	CTCGAG	2 cut(s)	1545, 3452
SmiMI	CAYNNNNRTG	9 cut(s)	435, 792, 918, 939, 2166, 2919, 4187, 5544, 6065
SnaBI	TACGTA	2 cut(s)	97, 233
SseBI	AGGCCT	1 cut(s)	1723
SstI	GAGCTC	3 cut(s)	1552, 4725, 6081
StuI	AGGCCT	1 cut(s)	1723
StyI	CCWWGG	8 cut(s)	163, 294, 605, 1727, 3153, 4650, 4907, 5571
TaqII	GACCGA	2 cut(s)	509, 4593
TatI	WGTACW	8 cut(s)	1092, 1113, 1500, 3954, 4453, 4516, 5467, 5598
TauI	GCSGC	3 cut(s)	21, 1037, 1784
TspGWI	ACGGA	1 cut(s)	2333
Van91I	CCANNNNNTGG	2 cut(s)	611, 1397
VneI	GTGCAC	1 cut(s)	3210
VspI	ATTAAT	2 cut(s)	572, 4221
XagI	CCTNNNNNAGG	1 cut(s)	3323
XapI	RAATTY	8 cut(s)	1233, 1587, 1593, 1605, 4704, 4727, 5611, 6377
XceI	RCATGY	5 cut(s)	434, 623, 684, 1526, 1646
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1164, 1205, 4325, 5429
XhoI	CTCGAG	2 cut(s)	1545, 3452
XmiI	GTMKAC	7 cut(s)	3640, 3730, 3923, 3966, 4500, 4829, 6357
XmnI	GAANNNNTTC	2 cut(s)	3115, 3437
ZraI	GACGTC	2 cut(s)	1299, 2742
ZrmI	AGTACT	3 cut(s)	1115, 1502, 5600
Zsp2I	ATGCAT	5 cut(s)	466, 493, 3606, 4344, 4921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.