Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

44046963 .. 44060556

Gene Structure 13594 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1363500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6711 bp

ATGGCATCTAAAGTTGATGAATTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATTGCAAACAATGGAATGGCTGCAGTCAAGTTTATTCGTAGTGTGAGGACATGGTCTTATGAGACATTTGGTACAGAGAAGGCTATTTTATTGGTGGCAATGGCCACACCAGAGGACATGAGAATCAATGCAGAACACATCAGAATCGCCGATCAGTTTGTGGAGGTTCCTGGTGGAACTAACAATAATAATTATGCCAATGTGCAACTCATTACAGAGATGGCAGAGAGAACACATGTTAATGCAGTTTGGCCCGGTTGGGGTCATGCCTCTGAGAACCCTGAGCTTCCAGATGCATTGAATGCAAAGGGAATTGTATTTCTTGGGCCACCATCTATATCTATGGCAGCATTAGGAGATAAAATTGGTTCATCATTAATTGCTCAGGCTGCCAACGTACCTACTCTTCCATGGAGCGGATCACATGTGAAAATTCCTTCAGATAGCTGCTTGGTTACAATCCCAGAGGAAGTATATAGGGAAGCGTGTGTTTATACAACAGAGGAAGCTGTTGCAAGTTGTCAGGTTGTAGGTTACCCAGCAATGATTAAGGCTTCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAGGTAAGGGCATTGTTCAAGCAAGTTCAGGGTGAAGTTCCTGGATCTCCAATATTTATAATGAAACTCGCAGCCCAGAGCCGACATTTAGAGGTACAGTTACTGTGTGATCAGTATGGAAATGTTTCAGCTTTGCATAGCCGTGATTGCAGTGTTCAAAGGCGGCACCAGAAGATTATTGAGGAGGGTCCAATTACTGTAGCTCCTATAGAGACTGTAAAAAAGTTGGAGCAATCAGCTCGAAGGTTGGCTAAATCTGTTAATTATGTTGGAGCAGCTACTGTTGAGTACCTCTACGGTATGGAAACTGGCGAGTACTATTTCTTGGAGCTGAACCCTCGATTACAGGTGGAGCACCCAGTCACTGAGTGGATAGCTGAAATAAATCTTCCAGCTGCCCAAGTTGTTGTTGGGATGGGCATACCTCTCTGGCAAATCCCTGAGATAAGGCGATTTTATGGAATGGAACATGGTGGTGGGTATGATGCTTGGAGAAAGACCTCAGCCATTGCTACCCCATTCGATTTTGACGAGGCAGAATCTACAAGGCCAAAGGGTCATTGTGTTGCCGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAGCCTACCAGTGGGAAAGTACAGGAGTTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCCTACTTTTCTGTTAAGTCTGGAGGGGGCATTCATGAATTCTCAGACTCTCAGTTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTAGAGGAGAAGTTCGTACAAATGTTGATTATTCAATAGATCTTCTACATGCCTCAGACTATAGAGAGAATAAAATCCACACGGGTTGGTTGGATAGTAGAATTGCCATGCGTGTTAGAGCAGAAAGGCCTCCTTGGTATCTTTCTGTAGTTGGAGGGACTTTGTTTAAAGCATCTGCTAGTAGTGCAGCAATGGTGTCAGATTATGTGGGTTATCTTGAGAAAGGGCAAATTCCACCCAAGCACATATCACATGTCCATGCACAAGTATCGTTGAACATTGAAGGGAGCAAATACACGATTGACATGGTGAGGGGGGGTCCAGGAAGCTACAGATTGAGGATGAATGAGTCAGAGATTGAAGCAGAGATACATACTTTACGCGATGGGGGTTTGTTGATGCAGTTGGATGGGAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGCCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGACCATGATCCGTCCAAGTTAATAGCAGAGACACCATGCAAGCTGCTGAGGTACTTGGTTACTGATGGTAGTCATGTCGAAGGTGACACACCATATGCCGAGGTTGAGGTTATGAAGATGTGTATGCCTCTTCTTTCACCTGCTTCTGGAGCTATCCACTTTAGAATGTCTGAAGGTCAAGCAATGCAGGCTGGTGAACTTATTGCAAAGCTTGATTTAGATGATCCTTCAGCTGTAAGAAAGGCAGAACCTTTCCATGGAAGCTTCCCAGTTCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTTCACCAGAGATGTGCTGCTAGCATAATTGCGGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCACTTGCCTAGACAGTCCTGAACTCCCTTTCCTTCAATGGCAAGAGTGCTTTGCTGTTCTAGCAACCCGCCTACCCAAAGATCTTAAAAATGAATTGGAATCAAAATTTAAGGAGTTTGAGGTGATTTCTAGTTCTCAGAATGTTGAGTTCCCCGCTAAGTTGTTGCGCGGTGTTCTTGAGGCCCATCTATTCTCCTGTCCTGATAAAGAGAAAGGAGCCCAAGAAAGGCTTGTTGAGCCTTTGATGAGCCTTGTGAAGTCTTATGAGGGTGGACGAGAAAGTCATGCCCGAGTTATCGTCCAATCCCTTTTTGAAGAGTACCTGTCTGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAACGCCTTCGACTTCAATATAAAAAAGATCTATTGAAGATTGTGGACATTGTGCTTTCTCACCAGGGTGTCAAGAATAAAAATAAGTTGATACTACGACTCATGGAACAACTGGTCTACCCCAACCCTGCTGCGTACAGGGAAAAGCTAATTCGGTTTTCTCAACTTAACCATACAAGCTATTCTGAGTTGGCACTAAAGGCAAGTCAACTGATGGAGCAAACCAAGTTGAGTGAACTTCGTTCCAGCATTGCCCGAAGTCTTTCTGAGTTAGAGATGTTTACCGAGGATGGTGAAACCATGGATACTCCCAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCTCCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTATTTGATCACAGTGATCACACACTTCAGAGGCGGGTTGTGGAGAGCTATGTGCGTCGGTTATACCAGCCATATCTTGTAAAGGGGAGTGTGAGGATGCAGTGGCAAAGATCTGGTCTTATGGCTTCCTGGGAGTTCTTGGACGAACATACAGAGAGAAAAAATGCAAATGAAGATCAATCTTCTGGTAAATCAATTGAGAAATACAGCGAGAGAAAATGGGGAGTCATGATTATTATCAAATCCCTTCAATTTCTTCCAGCAATTATTAGTGCTGCATTGAAGGAAATGTCTCATCAACTTCATGAATCAATTCCGAATGGATCTAGTGATCCAAGCAGCTTTGGAAATATGATGCATATTGCTTTAGTTGGCATCAACAATCAGATGAGTTTACTTCAGGATAGTGGCGATGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCGAAAATACTAAAAGAGCAAGGAGTAGCGTCCAGTCTGCACAGTGCAGGTGTCAGGGTAATTAGTTGCATCATACAAAGAGATGAAGGGCGAGCTCCTATGAGGCATTCCTTCCACTGGTCGTCAGAGAAACTCTATTACAAGGAAGAGCCATTATTGCGTCATCTGGAACCACCTCTATCCATTTACCTTGAGTTGGACAAGCTTAAAGGTTACGAGAATGTACAGTATACCCCATCCCGGGATCGTCAGTGGCACTTGTACAGCGTTGTAGATAAGCCACAACCAACCAAAAGGATGTTCCTCAGAACACTTGTAAGGCAGCCCACTTCAAATGATGGGTTTGCTGGATTTCAAAGGCTAGACATGGAAGCAGCTAATAAGCAATGGACTTTGTCATTTACTTCAAGGAGTATTTTAAGGTCCTTATTGACAGCAATGGAAGAGTTGGAACTTAATGCACACAATGCCAGTGTCAAATCTGACCATACTCATATGTACCTTTATATCTTGCGTGAGCAACAAATTGATGATCTACTGCCTTACCCCAAGAGAGTTGATTTAGATTCCGGACAAGAAGAAACTGTGGTGGAGGCCATCTTAGAAGAACTTGCACGGGAAATCCATGCGTCTGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGCTGTGGATTGCATCCTCTCGGCAGGCAAATGTTGCTTGGAGGGTTGTCGTCACGAACGTGACTGGTCATACCTGTACTGTACATATATATCGGGAACTAGAAGATACCAGCAAACAGGGGGTGGTATACCATTCAGCCTCTACACAGCCCCCCCTGCATGGTTTACCAGTGAATGCACAGTACCAGCCTCTGGGAGCTATTGACCGCAGACGTCTGTTGGCCAGGAGAACCAGCACCACTTACTGCTATGATTTTCCACTGGCATTTCAGATGGCCTTGGAACAGGCATGGGCATCCCAGTTGCCAGGTAGTAAAAAACCAAAAGATAAAATTCTTAAAGTCACTGAGCTCAAGTTTTCTGACCAGCATGGCACCTGGGGCACTCCTCTTGTTGAAGTGGTTCGTCCACCTGGGCTCAATGATGTTGGTATGGTAGCCTGGTTTATGGAGATGTCTACCCCTGAGTTCCCTTCAGGAAGGAAGATATTGATTGTTTCAAATGATGTGACCTTCAAAGCTGGATCTTTTGGTCCAAGAGAGGATGCATTCTTCTTTGCAGTAACTGAACTTGCTTGTGCTAAGAAACTGCCCTTAATATACTTGGCAGCAAACTCAGGTGCCCGTATTGGGGTAGCTGAAGAAGTCAAATCCTGCTTTAAAGTTGGTTGGTCTGATGAATCGAGTCCTGAGCGTGGCTTTCAGTATGTATATTTAACCTCTGAGGATTATGCTCGGATTGGATCATCTGTGATAGCACATGAATTAAAGCTGGCAAGTGGAGAAAGCAGATGGGTTATAGATACCATTGTTGGAAAGGAGGATGGCTTGGGAGTTGAAAGCTTGACTGGCAGTGGAGCAATTGCTGGTGCATATTCAAGGGCATACAGGGAAACCTTTACCTTAACATACGTGACCGGCAGAACTGTAGGGATAGGGGCTTATCTTGCTCGGCTTGGGATGCGGTGCATACAGAGGCTTGATCAGCCCATTATTTTAACTGGTTTCTCTGCATTGAACAAACTTCTTGGCCGGGAGGTGTACAGCTCTCATATGCAACTTGGAGGACCTAAAATTATGGGAACAAATGGGGTTGTCCATCTAACGGTTGCTGATGATCTTGAAGGAATATCTGCTATTGTGAAGTGGTTAAGTTATGTTCCTGCCCATGCTGGTGGACCACTCCCCATTTTAAGTCCCTCAGATCCTCCAGAAAGGCCTGTTGAGTACTGCCCTGAGAATTCATGTGATCCTCGTGCTGCTATTAGCGGCACTTTAAATGGTGATGGAAAGTGGATGGGGGGTATTTTTGACAAAGACAGCTTTATTGAAACACTAGAAGGTTGGGCGAGGACGGTTGTTACTGGAAGGGCAAAGCTTGGAGGAATCCCTGTAGGAATAGTTGCTGTCGAGACCCAGACGGTGATGCAAGTTATACCTGCTGATCCAGGCCAGCTTGATTCGCATGAGAGGGTTGTTCCTCAGGCTGGGCAAGTATGGTTTCCTGATTCTGCTAGTAAGACAGCCCAAGCGTTATTAGATTTCAACAGAGAGGAGCTCCCACTTTTCATTCTTGCCAACTGGAGAGGATTTTCTGGTGGGCAGAGGGACCTTTTCGAAGGGATCCTTCAGGCTGGATCAACCATTGTGGAGAACCTTAGGACTTACAAACAGCCCATATTTGTGTTCATCCCCATGATGGGTGAACTCCGCGGTGGAGCGTGGGTGGTCGTGGATAGTCGAATAAACCCAGACCATATTGAAATGTATGCTGATCGAACTGCTAGAGGTAACGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTCAGGAACAAGGAGCTGCTGGAGAGCATGGGCAGGCTTGACCAACAGCTGATCCAATTGAAGGCAAAACTTCAGGAAGCCAAGAGCAGTGGGGCACATGAGATGGTTGAAACGCTACAGCATCAGATACGATCACGTGAAAAACAGCTTTTGCCTGTCTACACTCAGATAGCCACTAGATTTGCGGAACTGCACGATACTTCTCTCAGGATGGCTGCAAAGGGGGTAATCAGAGAAGTTTTGGACTGGAACAGTTCTCGATCTTTCTTCTATAAAAGATTGCGTCGGAGAATTTCCGAAGAGTCACTCATCAAGACAGTGAGAGATGCTGCTGGTGAACAGCTGTCGCATAAATCTGCTTCAGATTTGATCAAGAACTGGTTTTTGAGTTCTGATATTCCAGGATGCAGAGAAGATGCTTGGGTGGATGACGAAATTTTCTTTAGATGGAAGGAAAATCCAAAAAATTACGAGGATAAACTAAAGGAGTTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAAATATTGGTGACTCAGTTTCTGATTTGCAAGCCTTACCCCAAGGTCTTGCTGCCCTTCTAAGCAAGGTCGAGCCATCAAGCAGAGCGCTGTTGATTGATGAACTCCGAAGGGTGCTCGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2237

Amino Acids

249.69

Weight (kDa)

6.11

Isoelectric Point (pI)

42.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	18 - 136	4.6e-29	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	185 - 367	2.2e-44	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	186 - 337	5.2e-06	ATP-grasp domain
ATP-grasp_3	PF02655	193 - 339	9.8e-08	ATP-grasp domain
Biotin_carb_C	PF02785	414 - 520	1.8e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	536 - 651	1.4e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	661 - 724	6.6e-10	Biotin-requiring enzyme
ACC_central	PF08326	725 - 1467	2e-180	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1569 - 2120	2e-152	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	728
AarI	CACCTGC	2 cut(s)	2107, 3599
AasI	GACNNNNNNGTC	1 cut(s)	2634
AatII	GACGTC	1 cut(s)	4456
Acc36I	ACCTGC	3 cut(s)	2107, 3599, 5686
AccB1I	GGYRCC	3 cut(s)	834, 4613, 4859
AccB7I	CCANNNNNTGG	2 cut(s)	1268, 4432
AccBSI	CCGCTC	1 cut(s)	483
AccI	GTMKAC	5 cut(s)	2841, 3791, 4368, 4697, 6225
AccII	CGCG	3 cut(s)	1848, 2523, 5952
AccIII	TCCGGA	1 cut(s)	4130
AcoI	YGGCCR	3 cut(s)	168, 4461, 5269
AcvI	CACGTG	1 cut(s)	6203
AcyI	GRCGYC	1 cut(s)	4453
AdeI	CACNNNGTG	1 cut(s)	1038
AfeI	AGCGCT	1 cut(s)	6675
AflIII	ACRYGT	4 cut(s)	301, 490, 1393, 1717
AjuI	GAANNNNNNNTTGG	4 cut(s)	2236, 2268, 3845, 3877
AleI	CACNNNNGTG	2 cut(s)	2790, 4298
AloI	GAACNNNNNNTCC	2 cut(s)	2367, 2399
Alw21I	GWGCWC	7 cut(s)	1026, 1423, 3085, 3658, 4593, 5799, 6705
Alw26I	GTCTC	6 cut(s)	122, 875, 1419, 1982, 3411, 5645
AlwNI	CAGNNNCTG	6 cut(s)	772, 950, 1034, 4432, 4805, 6608
Ama87I	CYCGRG	3 cut(s)	1416, 2643, 3801
Aor13HI	TCCGGA	1 cut(s)	4130
Aor51HI	AGCGCT	1 cut(s)	6675
ArsI	GACNNNNNNTTYG	4 cut(s)	1370, 1402, 3214, 3246
AseI	ATTAAT	1 cut(s)	443
Asp700I	GAANNNNTTC	5 cut(s)	793, 2730, 2986, 3426, 4641
AspLEI	GCGC	2 cut(s)	2523, 6676
AsuC2I	CCSGG	4 cut(s)	321, 3802, 3803, 5273
AsuII	TTCGAA	1 cut(s)	5856
AsuNHI	GCTAGC	1 cut(s)	2282
AvaI	CYCGRG	3 cut(s)	1416, 2643, 3801
AvaII	GGWCC	9 cut(s)	655, 857, 1784, 3984, 4772, 5306, 5417, 5848, 6562
AxyI	CCTNAGG	2 cut(s)	5721, 5897
BaeGI	GKGCMC	3 cut(s)	4625, 4864, 6163
BaeI	ACNNNNGTAYC	2 cut(s)	5034, 5067
BalI	TGGCCA	2 cut(s)	170, 4463
BamHI	GGATCC	2 cut(s)	1258, 5862
BanI	GGYRCC	3 cut(s)	834, 4613, 4859
BanII	GRGCYC	6 cut(s)	1423, 2575, 3658, 4593, 4659, 5799
BarI	GAAGNNNNNNTAC	4 cut(s)	456, 488, 1818, 1850
BauI	CACGAG	1 cut(s)	5493
BbrPI	CACGTG	1 cut(s)	6203
Bbv12I	GWGCWC	7 cut(s)	1026, 1423, 3085, 3658, 4593, 5799, 6705
BbvCI	CCTCAGC	2 cut(s)	1171, 2006
BceAI	ACGGC	2 cut(s)	795, 1223
BcgI	CGANNNNNNTGC	4 cut(s)	890, 924, 1716, 1750
BciVI	GTATCC	1 cut(s)	3022
BclI	TGATCA	5 cut(s)	778, 3121, 3130, 5221, 6435
BcnI	CCSGG	4 cut(s)	321, 3802, 3803, 5273
BcoDI	GTCTC	6 cut(s)	122, 875, 1419, 1982, 3411, 5645
BfmI	CTRYAG	9 cut(s)	87, 867, 876, 1525, 1611, 1795, 5166, 5631, 6182
BfoI	RGCGCY	1 cut(s)	6677
BfuAI	ACCTGC	3 cut(s)	2107, 3599, 5686
BfuI	GTATCC	1 cut(s)	3022
BglII	AGATCT	4 cut(s)	1504, 2434, 2752, 3224
BmcAI	AGTACT	2 cut(s)	986, 5468
Bme18I	GGWCC	9 cut(s)	655, 857, 1784, 3984, 4772, 5306, 5417, 5848, 6562
BmeT110I	CYCGRG	3 cut(s)	1416, 2643, 3801
BmrI	ACTGGG	3 cut(s)	1022, 2222, 4534
BmtI	GCTAGC	1 cut(s)	2286
BmuI	ACTGGG	3 cut(s)	1022, 2222, 4534
BplI	GAGNNNNNCTC	2 cut(s)	5781, 5813
BpmI	CTGGAG	5 cut(s)	1374, 2127, 5433, 5842, 6107
Bpu10I	CCTNAGC	5 cut(s)	348, 450, 1171, 2006, 4929
Bpu14I	TTCGAA	1 cut(s)	5856
BpuEI	CTTGAG	6 cut(s)	1703, 2552, 3525, 3773, 4577, 6059
BpuMI	CCSGG	4 cut(s)	321, 3802, 3803, 5273
BsaAI	YACGTR	2 cut(s)	5152, 6203
BsaBI	GATNNNNATC	3 cut(s)	3350, 3531, 6059
BsaHI	GRCGYC	1 cut(s)	4453
BsaI	GGTCTC	1 cut(s)	5645
BsaWI	WCCGGW	1 cut(s)	4130
BsaXI	ACNNNNNCTCC	4 cut(s)	856, 886, 5949, 5979
Bse118I	RCCGGY	1 cut(s)	5156
Bse21I	CCTNAGG	2 cut(s)	5721, 5897
Bse3DI	GCAATG	8 cut(s)	171, 615, 1176, 1662, 2148, 2973, 3953, 4005
Bse8I	GATNNNNATC	3 cut(s)	3350, 3531, 6059
BseAI	TCCGGA	1 cut(s)	4130
BseJI	GATNNNNATC	3 cut(s)	3350, 3531, 6059
BseMI	GCAATG	8 cut(s)	171, 615, 1176, 1662, 2148, 2973, 3953, 4005
BseRI	GAGGAG	4 cut(s)	866, 1485, 4617, 5807
BseSI	GKGCMC	3 cut(s)	4625, 4864, 6163
BseYI	CCCAGC	2 cut(s)	604, 5726
BsgI	GTGCAG	4 cut(s)	1671, 3584, 3627, 6242
Bsh1236I	CGCG	3 cut(s)	1848, 2523, 5952
BshNI	GGYRCC	3 cut(s)	834, 4613, 4859
BsiHKAI	GWGCWC	7 cut(s)	1026, 1423, 3085, 3658, 4593, 5799, 6705
BsiHKCI	CYCGRG	3 cut(s)	1416, 2643, 3801
BsiSI	CCGG	5 cut(s)	321, 3802, 4131, 5157, 5272
BslFI	GGGAC	3 cut(s)	1636, 5421, 5861
BsmAI	GTCTC	6 cut(s)	122, 875, 1419, 1982, 3411, 5645
BsmFI	GGGAC	3 cut(s)	1636, 5421, 5861
BsmI	GAATGC	5 cut(s)	373, 1362, 3667, 4420, 4787
Bso31I	GGTCTC	1 cut(s)	5645
BsoBI	CYCGRG	3 cut(s)	1416, 2643, 3801
Bsp119I	TTCGAA	1 cut(s)	5856
Bsp13I	TCCGGA	1 cut(s)	4130
Bsp1407I	TGTACA	4 cut(s)	3784, 3822, 4321, 5280
Bsp19I	CCATGG	3 cut(s)	476, 2215, 3024
BspEI	TCCGGA	1 cut(s)	4130
BspFNI	CGCG	3 cut(s)	1848, 2523, 5952
BspHI	TCATGA	3 cut(s)	1366, 3342, 3418
BspMAI	CTGCAG	1 cut(s)	91
BspMI	ACCTGC	3 cut(s)	2107, 3599, 5686
BspOI	GCTAGC	1 cut(s)	2286
BspQI	GCTCTTC	1 cut(s)	3702
BspT104I	TTCGAA	1 cut(s)	5856
BspT107I	GGYRCC	3 cut(s)	834, 4613, 4859
BspTNI	GGTCTC	1 cut(s)	5645
BsrBI	CCGCTC	1 cut(s)	483
BsrDI	GCAATG	8 cut(s)	171, 615, 1176, 1662, 2148, 2973, 3953, 4005
BsrFI	RCCGGY	1 cut(s)	5156
BsrGI	TGTACA	4 cut(s)	3784, 3822, 4321, 5280
BssAI	RCCGGY	1 cut(s)	5156
BssNAI	GTATAC	2 cut(s)	3792, 4369
BssNI	GRCGYC	1 cut(s)	4453
BssSI	CACGAG	1 cut(s)	5493
BssT1I	CCWWGG	7 cut(s)	34, 476, 1598, 2215, 3024, 4518, 6628
Bst1107I	GTATAC	2 cut(s)	3792, 4369
Bst2BI	CACGAG	1 cut(s)	5493
Bst6I	CTCTTC	6 cut(s)	477, 2094, 2664, 3702, 3999, 6360
BstACI	GRCGYC	1 cut(s)	4453
BstAPI	GCANNNNNTGC	4 cut(s)	368, 2350, 4028, 4274
BstAUI	TGTACA	4 cut(s)	3784, 3822, 4321, 5280
BstBAI	YACGTR	2 cut(s)	5152, 6203
BstBI	TTCGAA	1 cut(s)	5856
BstDSI	CCRYGG	4 cut(s)	476, 2215, 3024, 5950
BstEII	GGTNACC	1 cut(s)	599
BstFNI	CGCG	3 cut(s)	1848, 2523, 5952
BstH2I	RGCGCY	1 cut(s)	6677
BstHHI	GCGC	2 cut(s)	2523, 6676
BstMAI	GTCTC	6 cut(s)	122, 875, 1419, 1982, 3411, 5645
BstNSI	RCATGY	5 cut(s)	305, 494, 1397, 1517, 1721
BstPI	GGTNACC	1 cut(s)	599
BstSFI	CTRYAG	9 cut(s)	87, 867, 876, 1525, 1611, 1795, 5166, 5631, 6182
BstSLI	GKGCMC	3 cut(s)	4625, 4864, 6163
BstUI	CGCG	3 cut(s)	1848, 2523, 5952
BstXI	CCANNNNNNTGG	2 cut(s)	1329, 5414
BstZ17I	GTATAC	2 cut(s)	3792, 4369
Bsu36I	CCTNAGG	2 cut(s)	5721, 5897
BsuI	GTATCC	1 cut(s)	3022
BtgI	CCRYGG	4 cut(s)	476, 2215, 3024, 5950
BtgZI	GCGATG	2 cut(s)	1863, 3540
BtsI	GCAGTG	4 cut(s)	826, 3221, 5098, 6160
BveI	ACCTGC	3 cut(s)	2107, 3599, 5686
CaiI	CAGNNNCTG	6 cut(s)	772, 950, 1034, 4432, 4805, 6608
CciI	TCATGA	3 cut(s)	1366, 3342, 3418
CfoI	GCGC	2 cut(s)	2523, 6676
Cfr10I	RCCGGY	1 cut(s)	5156
Cfr42I	CCGCGG	1 cut(s)	5953
Cfr9I	CCCGGG	1 cut(s)	3801
CseI	GACGC	5 cut(s)	3158, 3579, 3710, 4179, 6338
CspCI	CAANNNNNGTGG	8 cut(s)	1532, 1567, 5868, 5903, 6136, 6171, 6229, 6264
DraI	TTTAAA	4 cut(s)	1312, 1633, 4900, 5517
DraIII	CACNNNGTG	1 cut(s)	1038
DrdI	GACNNNNNNGTC	1 cut(s)	2634
DseDI	GACNNNNNNGTC	1 cut(s)	2634
EaeI	YGGCCR	3 cut(s)	168, 4461, 5269
Eam1104I	CTCTTC	6 cut(s)	477, 2094, 2664, 3702, 3999, 6360
EarI	CTCTTC	6 cut(s)	477, 2094, 2664, 3702, 3999, 6360
Ecl136II	GAGCTC	4 cut(s)	1421, 3656, 4591, 5797
Eco130I	CCWWGG	7 cut(s)	34, 476, 1598, 2215, 3024, 4518, 6628
Eco147I	AGGCCT	2 cut(s)	1594, 5458
Eco24I	GRGCYC	6 cut(s)	1423, 2575, 3658, 4593, 4659, 5799
Eco31I	GGTCTC	1 cut(s)	5645
Eco47I	GGWCC	9 cut(s)	655, 857, 1784, 3984, 4772, 5306, 5417, 5848, 6562
Eco47III	AGCGCT	1 cut(s)	6675
Eco53kI	GAGCTC	4 cut(s)	1421, 3656, 4591, 5797
Eco72I	CACGTG	1 cut(s)	6203
Eco81I	CCTNAGG	2 cut(s)	5721, 5897
Eco88I	CYCGRG	3 cut(s)	1416, 2643, 3801
Eco91I	GGTNACC	1 cut(s)	599
EcoICRI	GAGCTC	4 cut(s)	1421, 3656, 4591, 5797
EcoO109I	RGGNCCY	4 cut(s)	2237, 3984, 5306, 5848
EcoO65I	GGTNACC	1 cut(s)	599
EcoRI	GAATTC	3 cut(s)	20, 1370, 5479
EcoT14I	CCWWGG	7 cut(s)	34, 476, 1598, 2215, 3024, 4518, 6628
EcoT22I	ATGCAT	4 cut(s)	364, 3474, 4212, 4789
EcoT38I	GRGCYC	6 cut(s)	1423, 2575, 3658, 4593, 4659, 5799
ErhI	CCWWGG	7 cut(s)	34, 476, 1598, 2215, 3024, 4518, 6628
FalI	AAGNNNNNCTT	8 cut(s)	1582, 1614, 2568, 2600, 3090, 3122, 5850, 5882
FaqI	GGGAC	3 cut(s)	1636, 5421, 5861
FauI	CCCGC	4 cut(s)	2429, 2515, 3141, 6552
FauNDI	CATATG	3 cut(s)	2053, 4056, 5292
FbaI	TGATCA	5 cut(s)	778, 3121, 3130, 5221, 6435
FblI	GTMKAC	5 cut(s)	2841, 3791, 4368, 4697, 6225
FriOI	GRGCYC	6 cut(s)	1423, 2575, 3658, 4593, 4659, 5799
GlaI	GCGC	2 cut(s)	2522, 6675
GsaI	CCCAGC	2 cut(s)	608, 5730
GsuI	CTGGAG	5 cut(s)	1374, 2127, 5433, 5842, 6107
HaeII	RGCGCY	1 cut(s)	6677
HapII	CCGG	5 cut(s)	321, 3802, 4131, 5157, 5272
HgaI	GACGC	5 cut(s)	3158, 3579, 3710, 4179, 6338
HhaI	GCGC	2 cut(s)	2523, 6676
Hin1I	GRCGYC	1 cut(s)	4453
Hin6I	GCGC	2 cut(s)	2521, 6674
HinP1I	GCGC	2 cut(s)	2521, 6674
HincII	GTYRAC	1 cut(s)	2933
HindII	GTYRAC	1 cut(s)	2933
HindIII	AAGCTT	5 cut(s)	2168, 2221, 3764, 5080, 5615
HpaII	CCGG	5 cut(s)	321, 3802, 4131, 5157, 5272
Hpy99I	CGWCG	2 cut(s)	3174, 6354
HpyCH4IV	ACGT	6 cut(s)	462, 4299, 4453, 5151, 6033, 6202
HpySE526I	ACGT	6 cut(s)	462, 4299, 4453, 5151, 6033, 6202
Hsp92I	GRCGYC	1 cut(s)	4453
HspAI	GCGC	2 cut(s)	2521, 6674
Kpn2I	TCCGGA	1 cut(s)	4130
Ksp22I	TGATCA	5 cut(s)	778, 3121, 3130, 5221, 6435
KspI	CCGCGG	1 cut(s)	5953
LguI	GCTCTTC	1 cut(s)	3702
MaeII	ACGT	6 cut(s)	462, 4299, 4453, 5151, 6033, 6202
MbiI	CCGCTC	1 cut(s)	483
MfeI	CAATTG	4 cut(s)	3309, 5100, 6122, 6581
MlsI	TGGCCA	2 cut(s)	170, 4463
MluNI	TGGCCA	2 cut(s)	170, 4463
MlyI	GAGTC	8 cut(s)	1373, 1421, 1823, 2817, 3348, 4933, 6377, 6593
Mox20I	TGGCCA	2 cut(s)	170, 4463
Mph1103I	ATGCAT	4 cut(s)	364, 3474, 4212, 4789
MroI	TCCGGA	1 cut(s)	4130
MroXI	GAANNNNTTC	5 cut(s)	793, 2730, 2986, 3426, 4641
MscI	TGGCCA	2 cut(s)	170, 4463
Msp20I	TGGCCA	2 cut(s)	170, 4463
MspA1I	CMGCKG	6 cut(s)	1064, 1913, 2192, 5952, 6115, 6409
MspI	CCGG	5 cut(s)	321, 3802, 4131, 5157, 5272
MunI	CAATTG	4 cut(s)	3309, 5100, 6122, 6581
Mva1269I	GAATGC	5 cut(s)	373, 1362, 3667, 4420, 4787
MvnI	CGCG	3 cut(s)	1848, 2523, 5952
NciI	CCSGG	4 cut(s)	321, 3802, 3803, 5273
NcoI	CCATGG	3 cut(s)	476, 2215, 3024
NdeI	CATATG	3 cut(s)	2053, 4056, 5292
NheI	GCTAGC	1 cut(s)	2282
NmeAIII	GCCGAG	3 cut(s)	2083, 4240, 5170
NsiI	ATGCAT	4 cut(s)	364, 3474, 4212, 4789
NspI	RCATGY	5 cut(s)	305, 494, 1397, 1517, 1721
NspV	TTCGAA	1 cut(s)	5856
OliI	CACNNNNGTG	2 cut(s)	2790, 4298
PaeR7I	CTCGAG	1 cut(s)	1416
PagI	TCATGA	3 cut(s)	1366, 3342, 3418
PaqCI	CACCTGC	2 cut(s)	2107, 3599
PceI	AGGCCT	2 cut(s)	1594, 5458
PciI	ACATGT	4 cut(s)	301, 490, 1393, 1717
PciSI	GCTCTTC	1 cut(s)	3702
PcsI	WCGNNNNNNNCGW	2 cut(s)	1197, 4296
PctI	GAATGC	5 cut(s)	373, 1362, 3667, 4420, 4787
PdmI	GAANNNNTTC	5 cut(s)	793, 2730, 2986, 3426, 4641
PflFI	GACNNNGTC	2 cut(s)	118, 3955
PflMI	CCANNNNNTGG	2 cut(s)	1268, 4432
PfoI	TCCNGGA	3 cut(s)	709, 1786, 6466
PleI	GAGTC	8 cut(s)	1373, 1420, 1822, 2817, 3347, 4932, 6376, 6593
PmaCI	CACGTG	1 cut(s)	6203
PmlI	CACGTG	1 cut(s)	6203
PpsI	GAGTC	8 cut(s)	1373, 1420, 1822, 2817, 3347, 4932, 6376, 6593
Ppu21I	YACGTR	2 cut(s)	5152, 6203
PpuMI	RGGWCCY	3 cut(s)	3984, 5306, 5848
PscI	ACATGT	4 cut(s)	301, 490, 1393, 1717
PshBI	ATTAAT	1 cut(s)	443
PsiI	TTATAA	1 cut(s)	728
Psp124BI	GAGCTC	4 cut(s)	1423, 3658, 4593, 5799
Psp5II	RGGWCCY	3 cut(s)	3984, 5306, 5848
PspCI	CACGTG	1 cut(s)	6203
PspEI	GGTNACC	1 cut(s)	599
PspFI	CCCAGC	2 cut(s)	604, 5726
PspPPI	RGGWCCY	3 cut(s)	3984, 5306, 5848
PstI	CTGCAG	1 cut(s)	91
PstNI	CAGNNNCTG	6 cut(s)	772, 950, 1034, 4432, 4805, 6608
PsyI	GACNNNGTC	2 cut(s)	118, 3955
PvuII	CAGCTG	5 cut(s)	1064, 1913, 2192, 6115, 6409
SacI	GAGCTC	4 cut(s)	1423, 3658, 4593, 5799
SacII	CCGCGG	1 cut(s)	5953
SapI	GCTCTTC	1 cut(s)	3702
ScaI	AGTACT	2 cut(s)	986, 5468
SchI	GAGTC	8 cut(s)	1373, 1421, 1823, 2817, 3348, 4933, 6377, 6593
SfcI	CTRYAG	9 cut(s)	87, 867, 876, 1525, 1611, 1795, 5166, 5631, 6182
Sfr274I	CTCGAG	1 cut(s)	1416
Sfr303I	CCGCGG	1 cut(s)	5953
SfuI	TTCGAA	1 cut(s)	5856
SgrBI	CCGCGG	1 cut(s)	5953
SinI	GGWCC	9 cut(s)	655, 857, 1784, 3984, 4772, 5306, 5417, 5848, 6562
SlaI	CTCGAG	1 cut(s)	1416
SmaI	CCCGGG	1 cut(s)	3803
SmlI	CTYRAG	7 cut(s)	1416, 1682, 2531, 3540, 3752, 4592, 6038
SmoI	CTYRAG	7 cut(s)	1416, 1682, 2531, 3540, 3752, 4592, 6038
SseBI	AGGCCT	2 cut(s)	1594, 5458
SspI	AATATT	2 cut(s)	723, 6592
SstI	GAGCTC	4 cut(s)	1423, 3658, 4593, 5799
StuI	AGGCCT	2 cut(s)	1594, 5458
StyI	CCWWGG	7 cut(s)	34, 476, 1598, 2215, 3024, 4518, 6628
TaiI	ACGT	6 cut(s)	465, 4302, 4456, 5154, 6036, 6205
TatI	WGTACW	8 cut(s)	984, 1294, 3784, 3822, 4316, 4321, 5280, 5466
TauI	GCSGC	3 cut(s)	835, 2297, 5511
TspGWI	ACGGA	1 cut(s)	1959
TspMI	CCCGGG	1 cut(s)	3801
Tth111I	GACNNNGTC	2 cut(s)	118, 3955
Van91I	CCANNNNNTGG	2 cut(s)	1268, 4432
VpaK11BI	GGWCC	9 cut(s)	655, 857, 1784, 3984, 4772, 5306, 5417, 5848, 6562
VspI	ATTAAT	1 cut(s)	443
XceI	RCATGY	5 cut(s)	305, 494, 1397, 1517, 1721
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1035, 1076, 4193
XhoI	CTCGAG	1 cut(s)	1416
XmaI	CCCGGG	1 cut(s)	3801
XmiI	GTMKAC	5 cut(s)	2841, 3791, 4368, 4697, 6225
XmnI	GAANNNNTTC	5 cut(s)	793, 2730, 2986, 3426, 4641
ZraI	GACGTC	1 cut(s)	4454
ZrmI	AGTACT	2 cut(s)	986, 5468
Zsp2I	ATGCAT	4 cut(s)	364, 3474, 4212, 4789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.