Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

4337212 .. 4350727

Gene Structure 13516 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000000716

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6816 bp

ATGATATACCAATTTGAAGCACTTCGATCAAGGTTGAAGCTCCTGCTTCATCTTTCTTCACCAAGGATGTCGGAAGCTCAAAGGAGATTGGTGGCAACTGTTAGTCTTCCTCGTAATAATGGTTACGTAAATGGGGGACTATCAATCAGGTCCCCTGCTGCAGCACCTGCAGTTGAGGAGTTCTGCCATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATAGCAAACAACGGAATGGCAGCAGTCAAGTTTATACGTAGTGTGAGGACATGGGCTTATGAAACATTTGGTACAGAAAAGGCTGTTTTGTTGGTGGCCATGGCCACACCAGAAGACATGAGAATCAATGCAGAACACATTAGAATTGCTGATCAGTTTGTGGAAGTTCCTGGTGGAACCAACAATAATAACTATGCCAATGTGCAACTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCATCTGAGATCCCTGAGCTTCCAGATGCATTGACTGCTAAGGGCATCATATTTCTCGGGCCACCAGCTGTATCTATGGCAGCATTAGGAGATAAAATCGGTTCATCATTAATTGCTCAAGCTGCTGAAGTACCCACCCTTCCATGGAGTGGATCACATGTGAAAATTGCTTCAGACAGCTGCCTGGTTACGATCCCAGATGAAATATATCGGGAAGCATGTGTTTACACAACAGAGGAAGCGATTGCAAGTTGTCAGGTTGTTGGTTACCCAGCCATGATTAAGGCATCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAAGTTAGGGCATTGTTCAAGCAAGTCCAGGGTGAAGTTCCTGGATCTCCCATATTCATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAAGTCCAGTTACTATGTGATCAGCATGGAAATGTTGCAGCTTTGCACAGCCGTGATTGCAGTGTTCAAAGGAGGCACCAGAAGATTATTGAGGAGGGTCCAATAACTGTAGCTCCACCAGAGACAATTAAAAAGTTGGAGCAGGCGGCTCGAAGGTTGGCTAAAAGTGTTAACTATGTTGGAGCAGCTACTGTTGAGTATCTGTACAGTATGGACACTGGCGAGTACTATTTCTTAGAGTTGAACCCTCGGTTGCAGGTGGAGCACCCAGTCACTGAGTGGATAGCAGAAATAAATCTGCCAGCAGCCCAAGTTGCAGTTGGGATGGGCATTCCCCTCTGGCAAATTCCTGAGATAAGGCGGTTCTATGGAATGGAACATGGTAGCGGATATGATGCTTGGAGAAAGACGTCAGTTGTTGCCACCCCATTTGACTTTGACAAGGCAGAATCTACAAGGCCAAAAGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAACCTACTAGTGGGAAATCTGGAGGGGGGATTCATGAGTACTCAGATTCTCAGTTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAACATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTGGATTATTCAATAGATCTTTTACATGCTACAGATTACAGAGAAAATAAAATTCACACAGGTTGGTTGGATAGTAGAATTGCTATGCGAGTTAGAACAGAAAGGCCTCCTTGGTATTTGTCTGTAATTGGAGGGACTCTTTTTAAAGCATCTGCTAGTAGTGCGGCGATGGTTTCAGATTATATTGGCTATCTTGAGAAAGGGCAAATCCCACCTAAGCATATATCATTTGTCCATTCACAAGTGTCTTTGAACATTGAGGGGAGCAAATACACGATTGACTTGGTGAGAGGGGGTCCTGGGAGCTATAGATTGCGGATGAATGAGTTAGAGGTTGAAGCAGAGATACATACTTTACGCGATGGAGGATTACTGATGCAGTTGGATGGAAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGTCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAATGATCACGATCCATCCAAGTTAATAGCAGAGACACCATGCAAGCTGTTGAGGTTCTTGGTTGCTGATGATAGTCATCTCGATGCTGATACACCATATGCAGAGGTTGAGGTTATGAAGATGTGTATGCCTCTACTTTCACCTGCTTCTGGAGTTATCCACTTTCAATTGTCTGAAGGCCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCAGCTGTGAGAAAGACAGAACCTTTCCATGGAAACTTCCCCGTCCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTACATCAAAGATGTGCTGCAAGCTTAAACGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCAATTGCCTAGATAGTCCTGAACTCCCTTTCCTTCAATGGCAAGAATGCTTGGCTGTTTTGGCAACCCGCCTACCCAAGAATCTCAAAAATGAACTGGAATCAAAATTTAAGGATTTTGAGTTGATTTCTAGCTCTCAGACTGTTGATTTCCCTGCGAAATTGTTGCGTGGTGTTCTTGAGGCCCATCTATCCTCCTGTCCTGATAAAGAAAAAGGAGCCCAAGAAAGGCTTGTTGAACCTTTGATGAGCCTTGTGACATCTTATGAAGGTGGAAGAGAAAGTCATGCCCGAGTTATTGTTCAATCCCTCTTTGAAGAGTATCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAGCGCCTTCGACTTCAATATAAGAAAGATCTGTTGAAGGTTGTGGAAATTGTGCTCTCTCATCAGGGTGTAAAGAATAAAAATAAGTTGATTCTACGACTCATGGAACAACTGGTCTACCCCAACCCTGCTGCATACAGGGAGAAGCTAATTCGGTTTTCTCAACTTAACCATACAAACTATTCTCAGTTGGCACTAAAAGCAAGTCAGCTTATGGAGCAAACCAAGTTGAGTGAACTTCGTTCTAGCATTGCTAGAAGTCTTTCTGAGTTGGAGATGTTTACTGAGGATGGTGAAACCATGGATACTCCAAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCACCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTCTTTGATCATGGTGATCACACCCTTCAGAGGCGGGTTGTTGAGAGTTATGTACGCCGGTTATACCAGCCCTACCTTGTAAAGGGGAGTGTCAGGATGCAGTGGCACAGATCTGGTCTAATAGCTTCATGGGAGTTCTCGGAAGAACCTGTTGAGAGGAAACATGAGATTGAAGGTCAATCAGTTGATAAAACATTCCTTCAGAAACACGAGGAGAGGAAATGGGGAGTCATGGTTATAATAAAATCTCTTCAGTTTCTTCCAGCAATTATTAGTGGTGCACTGAAGGAAATGTCTCATAACCTTCATGAAGCAACTCCAGATGGATCTACTGAACCAACTGGCTTTGGTAATATGATGCATATTGCTTTGGTTGGCATCAACAATCAGATGAGTCTACTTCAGGATAGTGGTGACGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATACTTAAAGAGCAAGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCACAGTAATTAGTTGTATCATTCAAAGGGATGAAGGGCGGGCCCCTATGAGGCACTCCTTCCACTGGTCATCAGAGAAGCTCTATTATGAAGAAGAACCCCTATTGCGTCACCTAGAACCTCCTCTATCTATTTACCTTGAATTGGACAAGCTAAAAGGCTATGAGAATATACAGTATACCCCATCTCGTGACCGTCAATGGCACTTGTACACTGTTGTAGACAAGCCAGTACCAATCCAAAGGATGTTCCTCAGAACACTTGTCAGGCAGCCCACAACAAATGAAGGGTTCTCTGGATTTCAACGGCTAGACATTGCAACAGCTAGTAACCAATTTTCTTTATCATTTACTTCGAGGAGCATCTTGAGGTCCTTATCAACAGCAATGGAAGAGTTGGAACTGAATGCTCACAATGCCACTGTCAAATCTGACCACACCCATATGTATCTTTATATCTTACGTGAGCAACAAATTAATGATCTGCTGCCTTACCACAAGAGAGTTGATTTAGATGCTCAACAAGAAGAAACTGTTGTGGAGGCCATCTTGGAAGAACTTGCACGTGAAATCCACACATCAGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGCTGTGGATGGCATCCTCTGGAGAGGCAAACGTTGCTTGGAGGGTTGTCGTGACAAATGTGACTGGTCATACCTGCACTGTACATATATATCGGGAACTAGAAGATACCAGCAAACAGGGAGCGGTATACCATTCAGTCTCTGTACAGGGTCCCCTGCATGGTGTACCAGTGAGTGCGCACTATCAGCCTTTGGGAATTATAGACCGAAAACGTCTGTTGGCTAGGAGAACCAACACCACTTACTGCTATGATTTTCCACTGGCATTTGAGACGGCCTTGGAACAGTCATGGGCATCCCAGTCACCAGGTGTTGACAAACCGAAGGGTAAAATTCTCAAGGTCACAGAGCTCAAATTCGCTGACCAAAAAGGCAGCTGGGGTACTCCTCTCAATACCGTAGAGCGTCCACCTGGACTCAATGATGTTGGTATGATAGCCTGGTTTATGGAGATGTCTACCCCTGAGTTCCCTTCTGGAAGGAGGATATTGGTTGTTGCGAATGATGTCACCTACAAAGCTGGATCTTTTGGCCCAAGGGAGGATGCATTCTTCTTTGCAGTAACTGAGCTTGCTTGTGCTGAGAAATTGCCTCTAATATACTTGGCAGCAAACTCAGGTGCCCGTATTGGGGTTGCTGAAGAAGTCAAATCCTGTTTTAAAGTGGGCTGGTCTGATGAATCAAGCCCCGAACGTGGTTTTCAATATGTGTATTTAACCTCTGAGGATTATGCTCGGATTGGATCATCTGTGATAGCGCATGAATTAAACCTGGCAAATGGTGAAACCAGATGGGTGATAGATACCATTGTTGGAAAGGAGAACGGCTTGGGGGTCGAGAGCTTGACTGGAAGTGGGGCAATTGCTGGTGCATATTCGAGGGCATATATGGAAACCTTTACCTTAACATATGTGACGGGCAGAACTGTAGGGATAGGTGCTTATCTTGCAAGGCTTGGGATGCGGTGCATCCAGAGGCTTGATCAGCCCATCATTTTAACTGGTTTCTCTGCATTAAACAAACTACTTGGCCGGGAGGTTTACAGCTCCCATATGCAACTTGGAGGACCTAAAATAATGGGAACTAATGGGGTTGTACATCTAACGGTAGCTGATGATCTTGAAGGAATATCTGCTATTCTAAAGTGGCTGAGCTATGTTCCTCCCCATGTTGGTGGTCCACTTCCCATTTCAATTCCCTTGGATCCTCCAGAAAGGCGTGTGGAGTACTGCCCCGAGAATTCATGTGATCCTCGTGCTGCGATTTCTGGTGCTCTGAATGGTAATGGGAACTGGATGGGGGGTATATTTGACAAAGACAGCTTTGTTGAGACGCTAGAAGGCTGGGCAAGGACGGTTGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTGGGAATTGTTGCTGTTGAGACACAGACGGTGATGCAAATTATTCCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGACAAGTATGGTTTCCTGATTCTGCAACTAAGACTGCCCAAGCATTATTAGATTTCAACAGAGAAGGACTCCCACTTTTCATTCTTGCCAACTGGAGAGGCTTTTCTGGTGGGCAGAGAGACCTTTTCGAAGGGATCCTTCAGGCTGGATCAACCATTGTGGAGAACCTAAGGACTTACAAACAGCCCATTTTTGTATTCATCCCAATGATGGGTGAGCTCCGCGGTGGAGCATGGGTGGTCGTTGATAGCCGGATAAATCCGGACCATATTGAAATGTATGCTGATCGAACGGCTAGAGGAAATGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTTAGGAACAAGGAATTGCTGGAGTGCATGGGCAGGCTTGACCAACAGCTGATCCAACTAAAGACTAAGCTTCAGGAAGCCAGGAGCTGTGGGGTGAATGAGAAGGTTGAATCCCTACAGTTTCAGATACGATCCCGAGAAAAACAGCTTTTGCCCGTCTACACTCAGATAGCTACCAAATTTGCAGAACTTCATGATACGTCTCTGAGGATGGCTGCAAAAGGGGTAATCAGAGGAGTTTTGGAGTGGGCCAGCTCTCGATCTTTCTTCTACAAGAGATTGCGTCGGAGAATTGCCGATGACTCACTCATCAAGACAGTGATAGATGCTGCTGGCGAACAGCTGTCACATAGATCTGCAATGGATTTGATCAAGAGCTGGTTTTTGAGTTCTGATATTTCAAGAGGCAGGGAAGACGCTTGGGAGGATGATGAAACTTTCTTTAGATGGAAGGATGATCAAACTAATTATGAGGGTAAGCTAAAAGAATTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAACCATTGGCAACTCAGCTTCTGATTTGCAAGCCCTACCTCAAGGTCTTGCTGCCCTTCTAAGCAAGGTTGAGCCATCAAGCAGATCATTTTTGGTTGAGGAACTCCGGAAGGTGCTTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2272

Amino Acids

253.09

Weight (kDa)

5.99

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	71 - 190	5.1e-28	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	238 - 420	5e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	239 - 390	2.7e-07	ATP-grasp domain
ATP-grasp_3	PF02655	245 - 392	2.4e-07	ATP-grasp domain
Biotin_carb_C	PF02785	485 - 554	3.1e-10	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	570 - 685	1.1e-27	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	697 - 758	8.3e-08	Biotin-requiring enzyme
ACC_central	PF08326	759 - 1502	7.6e-179	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1604 - 2155	4.6e-149	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3455
AarI	CACCTGC	4 cut(s)	175, 1165, 2209, 3704
AatII	GACGTC	1 cut(s)	1331
Acc16I	TGCGCA	1 cut(s)	4524
Acc36I	ACCTGC	5 cut(s)	175, 1165, 2209, 3704, 4427
AccB1I	GGYRCC	2 cut(s)	993, 4964
AccB7I	CCANNNNNTGG	2 cut(s)	641, 1427
AccBSI	CCGCTC	1 cut(s)	4469
AccI	GTMKAC	8 cut(s)	2943, 3613, 3703, 3896, 3939, 4473, 4802, 6330
AccII	CGCG	2 cut(s)	1950, 6057
AccIII	TCCGGA	2 cut(s)	6094, 6798
AclI	AACGTT	1 cut(s)	4377
AcoI	YGGCCR	3 cut(s)	321, 327, 5374
AcvI	CACGTG	1 cut(s)	4283
AcyI	GRCGYC	1 cut(s)	1328
AdeI	CACNNNGTG	2 cut(s)	1197, 4655
AflIII	ACRYGT	3 cut(s)	460, 649, 1495
AhlI	ACTAGT	1 cut(s)	1442
AjuI	GAANNNNNNNTTGG	2 cut(s)	2338, 2370
AleI	CACNNNNGTG	1 cut(s)	969
AloI	GAACNNNNNNTCC	2 cut(s)	2469, 2501
Alw21I	GWGCWC	8 cut(s)	1185, 1525, 2883, 3187, 3499, 4698, 5620, 6054
Alw44I	GTGCAC	2 cut(s)	3183, 3495
AlwNI	CAGNNNCTG	7 cut(s)	167, 1109, 1193, 4487, 4910, 6258, 6713
Ama87I	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
Aor13HI	TCCGGA	2 cut(s)	6094, 6798
ApaI	GGGCCC	1 cut(s)	3763
ApaLI	GTGCAC	2 cut(s)	3183, 3495
AseI	ATTAAT	2 cut(s)	602, 4194
Asp700I	GAANNNNTTC	2 cut(s)	21, 3088
AspLEI	GCGC	3 cut(s)	2832, 4525, 5104
AsuC2I	CCSGG	1 cut(s)	5378
AsuII	TTCGAA	1 cut(s)	5961
AvaI	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
AxyI	CCTNAGG	2 cut(s)	5826, 6002
BaeGI	GKGCMC	4 cut(s)	3187, 3499, 3763, 4969
BaeI	ACNNNNGTAYC	2 cut(s)	5139, 5172
BalI	TGGCCA	2 cut(s)	323, 329
BamHI	GGATCC	3 cut(s)	1417, 5548, 5967
BanI	GGYRCC	2 cut(s)	993, 4964
BanII	GRGCYC	5 cut(s)	1525, 2677, 3763, 4698, 6054
BarI	GAAGNNNNNNTAC	6 cut(s)	615, 647, 912, 944, 1920, 1952
BauI	CACGAG	3 cut(s)	3425, 3906, 5598
BbrPI	CACGTG	1 cut(s)	4283
BbsI	GAAGAC	4 cut(s)	98, 345, 2018, 6591
Bbv12I	GWGCWC	8 cut(s)	1185, 1525, 2883, 3187, 3499, 4698, 5620, 6054
BceAI	ACGGC	5 cut(s)	954, 4040, 4635, 5185, 6141
BcgI	CGANNNNNNTGC	4 cut(s)	1049, 1083, 2602, 2636
BciVI	GTATCC	1 cut(s)	3124
BclI	TGATCA	8 cut(s)	376, 937, 2062, 3223, 3232, 5326, 6540, 6628
BcnI	CCSGG	1 cut(s)	5378
BcuI	ACTAGT	1 cut(s)	1442
BfmI	CTRYAG	7 cut(s)	159, 168, 1026, 1620, 1897, 5271, 6287
BfoI	RGCGCY	1 cut(s)	2833
BfuAI	ACCTGC	5 cut(s)	175, 1165, 2209, 3704, 4427
BfuI	GTATCC	1 cut(s)	3124
BglII	AGATCT	4 cut(s)	1606, 2854, 3326, 6524
BlpI	GCTNAGC	1 cut(s)	5495
BmcAI	AGTACT	3 cut(s)	1145, 1475, 5573
BmeT110I	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
BmrI	ACTGGG	2 cut(s)	1181, 4639
BmuI	ACTGGG	2 cut(s)	1181, 4639
BpiI	GAAGAC	4 cut(s)	98, 345, 2018, 6591
BplI	GAGNNNNNCTC	2 cut(s)	5886, 5918
BpmI	CTGGAG	8 cut(s)	1476, 2229, 3519, 3682, 4386, 5538, 5947, 6212
Bpu10I	CCTNAGC	3 cut(s)	507, 531, 1806
Bpu1102I	GCTNAGC	1 cut(s)	5495
Bpu14I	TTCGAA	1 cut(s)	5961
BpuEI	CTTGAG	8 cut(s)	594, 1805, 2654, 3630, 4105, 4667, 6164, 6717
BpuMI	CCSGG	1 cut(s)	5378
BsaAI	YACGTR	4 cut(s)	127, 263, 4181, 4283
BsaBI	GATNNNNATC	3 cut(s)	2067, 2133, 6164
BsaHI	GRCGYC	1 cut(s)	1328
BsaI	GGTCTC	2 cut(s)	3221, 5946
BsaWI	WCCGGW	2 cut(s)	6094, 6798
BsaXI	ACNNNNNCTCC	6 cut(s)	1015, 1045, 3435, 3465, 6054, 6084
Bse118I	RCCGGY	1 cut(s)	3273
Bse21I	CCTNAGG	2 cut(s)	5826, 6002
Bse3DI	GCAATG	5 cut(s)	2250, 3075, 4032, 4110, 6537
Bse8I	GATNNNNATC	3 cut(s)	2067, 2133, 6164
BseAI	TCCGGA	2 cut(s)	6094, 6798
BseJI	GATNNNNATC	3 cut(s)	2067, 2133, 6164
BseMI	GCAATG	5 cut(s)	2250, 3075, 4032, 4110, 6537
BseRI	GAGGAG	8 cut(s)	191, 1025, 1587, 3443, 3831, 4090, 4722, 6420
BseSI	GKGCMC	4 cut(s)	3187, 3499, 3763, 4969
BseYI	CCCAGC	3 cut(s)	763, 4722, 5688
BsgI	GTGCAG	2 cut(s)	3732, 4405
Bsh1236I	CGCG	2 cut(s)	1950, 6057
BshNI	GGYRCC	2 cut(s)	993, 4964
BsiHKAI	GWGCWC	8 cut(s)	1185, 1525, 2883, 3187, 3499, 4698, 5620, 6054
BsiHKCI	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
BsiSI	CCGG	5 cut(s)	3274, 5377, 6085, 6095, 6799
BslFI	GGGAC	4 cut(s)	136, 150, 1738, 4482
BsmBI	CGTCTC	3 cut(s)	4610, 5669, 6378
BsmFI	GGGAC	4 cut(s)	136, 150, 1738, 4482
BsmI	GAATGC	4 cut(s)	1248, 2507, 4129, 4892
Bso31I	GGTCTC	2 cut(s)	3221, 5946
BsoBI	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
Bsp119I	TTCGAA	1 cut(s)	5961
Bsp120I	GGGCCC	1 cut(s)	3759
Bsp13I	TCCGGA	2 cut(s)	6094, 6798
Bsp1407I	TGTACA	5 cut(s)	1122, 3927, 4426, 4489, 5440
Bsp1720I	GCTNAGC	1 cut(s)	5495
Bsp19I	CCATGG	4 cut(s)	324, 635, 2317, 3126
BspEI	TCCGGA	2 cut(s)	6094, 6798
BspFNI	CGCG	2 cut(s)	1950, 6057
BspHI	TCATGA	3 cut(s)	1468, 3523, 6364
BspMAI	CTGCAG	2 cut(s)	163, 172
BspMI	ACCTGC	5 cut(s)	175, 1165, 2209, 3704, 4427
BspT104I	TTCGAA	1 cut(s)	5961
BspT107I	GGYRCC	2 cut(s)	993, 4964
BspTNI	GGTCTC	2 cut(s)	3221, 5946
BsrBI	CCGCTC	1 cut(s)	4469
BsrDI	GCAATG	5 cut(s)	2250, 3075, 4032, 4110, 6537
BsrFI	RCCGGY	1 cut(s)	3273
BsrGI	TGTACA	5 cut(s)	1122, 3927, 4426, 4489, 5440
BssAI	RCCGGY	1 cut(s)	3273
BssNAI	GTATAC	2 cut(s)	3897, 4474
BssNI	GRCGYC	1 cut(s)	1328
BssSI	CACGAG	3 cut(s)	3425, 3906, 5598
Bst1107I	GTATAC	2 cut(s)	3897, 4474
Bst2BI	CACGAG	3 cut(s)	3425, 3906, 5598
Bst6I	CTCTTC	4 cut(s)	2725, 2766, 3471, 4104
BstACI	GRCGYC	1 cut(s)	1328
BstAPI	GCANNNNNTGC	4 cut(s)	167, 527, 2452, 4379
BstAUI	TGTACA	5 cut(s)	1122, 3927, 4426, 4489, 5440
BstBAI	YACGTR	4 cut(s)	127, 263, 4181, 4283
BstBI	TTCGAA	1 cut(s)	5961
BstDSI	CCRYGG	5 cut(s)	324, 635, 2317, 3126, 6055
BstEII	GGTNACC	1 cut(s)	758
BstENI	CCTNNNNNAGG	1 cut(s)	3296
BstFNI	CGCG	2 cut(s)	1950, 6057
BstH2I	RGCGCY	1 cut(s)	2833
BstHHI	GCGC	3 cut(s)	2832, 4525, 5104
BstNSI	RCATGY	5 cut(s)	464, 653, 714, 1499, 1619
BstPI	GGTNACC	1 cut(s)	758
BstSFI	CTRYAG	7 cut(s)	159, 168, 1026, 1620, 1897, 5271, 6287
BstSLI	GKGCMC	4 cut(s)	3187, 3499, 3763, 4969
BstSNI	TACGTA	2 cut(s)	127, 263
BstUI	CGCG	2 cut(s)	1950, 6057
BstV2I	GAAGAC	4 cut(s)	98, 345, 2018, 6591
BstXI	CCANNNNNNTGG	2 cut(s)	194, 5519
BstZ17I	GTATAC	2 cut(s)	3897, 4474
Bsu36I	CCTNAGG	2 cut(s)	5826, 6002
BsuI	GTATCC	1 cut(s)	3124
BtgI	CCRYGG	5 cut(s)	324, 635, 2317, 3126, 6055
BtgZI	GCGATG	2 cut(s)	1772, 1965
BtsI	GCAGTG	2 cut(s)	985, 3323
BveI	ACCTGC	5 cut(s)	175, 1165, 2209, 3704, 4427
CaiI	CAGNNNCTG	7 cut(s)	167, 1109, 1193, 4487, 4910, 6258, 6713
CciI	TCATGA	3 cut(s)	1468, 3523, 6364
CfoI	GCGC	3 cut(s)	2832, 4525, 5104
Cfr10I	RCCGGY	1 cut(s)	3273
Cfr42I	CCGCGG	1 cut(s)	6058
CseI	GACGC	5 cut(s)	3815, 4739, 5686, 6443, 6596
CsiI	ACCWGGT	1 cut(s)	4651
CspCI	CAANNNNNGTGG	4 cut(s)	1330, 1365, 5973, 6008
DraI	TTTAAA	3 cut(s)	1435, 1735, 5005
DraIII	CACNNNGTG	2 cut(s)	1197, 4655
EaeI	YGGCCR	3 cut(s)	321, 327, 5374
Eam1104I	CTCTTC	4 cut(s)	2725, 2766, 3471, 4104
EarI	CTCTTC	4 cut(s)	2725, 2766, 3471, 4104
Ecl136II	GAGCTC	3 cut(s)	1523, 4696, 6052
Eco105I	TACGTA	2 cut(s)	127, 263
Eco147I	AGGCCT	1 cut(s)	1696
Eco24I	GRGCYC	5 cut(s)	1525, 2677, 3763, 4698, 6054
Eco31I	GGTCTC	2 cut(s)	3221, 5946
Eco53kI	GAGCTC	3 cut(s)	1523, 4696, 6052
Eco72I	CACGTG	1 cut(s)	4283
Eco81I	CCTNAGG	2 cut(s)	5826, 6002
Eco88I	CYCGRG	5 cut(s)	548, 1518, 2745, 5579, 6306
Eco91I	GGTNACC	1 cut(s)	758
EcoICRI	GAGCTC	3 cut(s)	1523, 4696, 6052
EcoNI	CCTNNNNNAGG	1 cut(s)	3296
EcoO109I	RGGNCCY	7 cut(s)	150, 1886, 2339, 3760, 4089, 4496, 5411
EcoO65I	GGTNACC	1 cut(s)	758
EcoRI	GAATTC	1 cut(s)	5584
EcoT22I	ATGCAT	5 cut(s)	496, 523, 3579, 4317, 4894
EcoT38I	GRGCYC	5 cut(s)	1525, 2677, 3763, 4698, 6054
Esp3I	CGTCTC	3 cut(s)	4610, 5669, 6378
FalI	AAGNNNNNCTT	8 cut(s)	1684, 1716, 2670, 2702, 3192, 3224, 5955, 5987
FaqI	GGGAC	4 cut(s)	136, 150, 1738, 4482
FauI	CCCGC	4 cut(s)	2531, 3243, 3750, 6657
FauNDI	CATATG	4 cut(s)	2155, 4161, 5254, 5397
FbaI	TGATCA	8 cut(s)	376, 937, 2062, 3223, 3232, 5326, 6540, 6628
FblI	GTMKAC	8 cut(s)	2943, 3613, 3703, 3896, 3939, 4473, 4802, 6330
FriOI	GRGCYC	5 cut(s)	1525, 2677, 3763, 4698, 6054
FspAI	RTGCGCAY	1 cut(s)	4524
FspI	TGCGCA	1 cut(s)	4524
GlaI	GCGC	3 cut(s)	2831, 4524, 5103
GsaI	CCCAGC	3 cut(s)	767, 4726, 5692
GsuI	CTGGAG	8 cut(s)	1476, 2229, 3519, 3682, 4386, 5538, 5947, 6212
HaeII	RGCGCY	1 cut(s)	2833
HapII	CCGG	5 cut(s)	3274, 5377, 6085, 6095, 6799
HgaI	GACGC	5 cut(s)	3815, 4739, 5686, 6443, 6596
HhaI	GCGC	3 cut(s)	2832, 4525, 5104
Hin1I	GRCGYC	1 cut(s)	1328
Hin6I	GCGC	3 cut(s)	2830, 4523, 5102
HinP1I	GCGC	3 cut(s)	2830, 4523, 5102
HincII	GTYRAC	2 cut(s)	1090, 4660
HindII	GTYRAC	2 cut(s)	1090, 4660
HindIII	AAGCTT	3 cut(s)	2386, 5720, 6239
HpaI	GTTAAC	1 cut(s)	1090
HpaII	CCGG	5 cut(s)	3274, 5377, 6085, 6095, 6799
Hpy99I	CGWCG	1 cut(s)	6459
HpyCH4IV	ACGT	9 cut(s)	126, 262, 1328, 4180, 4282, 4377, 4558, 5038, 6371
HpySE526I	ACGT	9 cut(s)	126, 262, 1328, 4180, 4282, 4377, 4558, 5038, 6371
Hsp92I	GRCGYC	1 cut(s)	1328
HspAI	GCGC	3 cut(s)	2830, 4523, 5102
KflI	GGGWCCC	1 cut(s)	4496
Kpn2I	TCCGGA	2 cut(s)	6094, 6798
Ksp22I	TGATCA	8 cut(s)	376, 937, 2062, 3223, 3232, 5326, 6540, 6628
KspAI	GTTAAC	1 cut(s)	1090
KspI	CCGCGG	1 cut(s)	6058
MabI	ACCWGGT	1 cut(s)	4651
MaeII	ACGT	9 cut(s)	126, 262, 1328, 4180, 4282, 4377, 4558, 5038, 6371
MbiI	CCGCTC	1 cut(s)	4469
MfeI	CAATTG	4 cut(s)	2225, 2458, 5205, 6686
MlsI	TGGCCA	2 cut(s)	323, 329
MluNI	TGGCCA	2 cut(s)	323, 329
MlyI	GAGTC	8 cut(s)	1523, 1720, 2919, 3453, 3619, 4755, 5895, 6467
Mox20I	TGGCCA	2 cut(s)	323, 329
Mph1103I	ATGCAT	5 cut(s)	496, 523, 3579, 4317, 4894
MroI	TCCGGA	2 cut(s)	6094, 6798
MroXI	GAANNNNTTC	2 cut(s)	21, 3088
MscI	TGGCCA	2 cut(s)	323, 329
MslI	CAYNNNNRTG	9 cut(s)	465, 822, 948, 969, 2139, 2892, 4160, 5517, 6038
Msp20I	TGGCCA	2 cut(s)	323, 329
MspA1I	CMGCKG	8 cut(s)	560, 672, 2015, 2294, 4722, 6057, 6220, 6514
MspI	CCGG	5 cut(s)	3274, 5377, 6085, 6095, 6799
MunI	CAATTG	4 cut(s)	2225, 2458, 5205, 6686
Mva1269I	GAATGC	4 cut(s)	1248, 2507, 4129, 4892
MvnI	CGCG	2 cut(s)	1950, 6057
NciI	CCSGG	1 cut(s)	5378
NcoI	CCATGG	4 cut(s)	324, 635, 2317, 3126
NdeI	CATATG	4 cut(s)	2155, 4161, 5254, 5397
NsbI	TGCGCA	1 cut(s)	4524
NsiI	ATGCAT	5 cut(s)	496, 523, 3579, 4317, 4894
NspI	RCATGY	5 cut(s)	464, 653, 714, 1499, 1619
NspV	TTCGAA	1 cut(s)	5961
OliI	CACNNNNGTG	1 cut(s)	969
PaeR7I	CTCGAG	1 cut(s)	1518
PagI	TCATGA	3 cut(s)	1468, 3523, 6364
PaqCI	CACCTGC	4 cut(s)	175, 1165, 2209, 3704
PceI	AGGCCT	1 cut(s)	1696
PciI	ACATGT	3 cut(s)	460, 649, 1495
PctI	GAATGC	4 cut(s)	1248, 2507, 4129, 4892
PdmI	GAANNNNTTC	2 cut(s)	21, 3088
PflMI	CCANNNNNTGG	2 cut(s)	641, 1427
PfoI	TCCNGGA	1 cut(s)	868
PleI	GAGTC	8 cut(s)	1522, 1720, 2919, 3452, 3618, 4755, 5895, 6467
PmaCI	CACGTG	1 cut(s)	4283
PmlI	CACGTG	1 cut(s)	4283
PpsI	GAGTC	8 cut(s)	1522, 1720, 2919, 3452, 3618, 4755, 5895, 6467
Ppu21I	YACGTR	4 cut(s)	127, 263, 4181, 4283
PpuMI	RGGWCCY	5 cut(s)	150, 1886, 4089, 4496, 5411
PscI	ACATGT	3 cut(s)	460, 649, 1495
PshBI	ATTAAT	2 cut(s)	602, 4194
PsiI	TTATAA	1 cut(s)	3455
Psp124BI	GAGCTC	3 cut(s)	1525, 4698, 6054
Psp1406I	AACGTT	1 cut(s)	4377
Psp5II	RGGWCCY	5 cut(s)	150, 1886, 4089, 4496, 5411
PspCI	CACGTG	1 cut(s)	4283
PspEI	GGTNACC	1 cut(s)	758
PspFI	CCCAGC	3 cut(s)	763, 4722, 5688
PspOMI	GGGCCC	1 cut(s)	3759
PspPPI	RGGWCCY	5 cut(s)	150, 1886, 4089, 4496, 5411
PstI	CTGCAG	2 cut(s)	163, 172
PstNI	CAGNNNCTG	7 cut(s)	167, 1109, 1193, 4487, 4910, 6258, 6713
PvuII	CAGCTG	7 cut(s)	560, 672, 2015, 2294, 4722, 6220, 6514
RseI	CAYNNNNRTG	9 cut(s)	465, 822, 948, 969, 2139, 2892, 4160, 5517, 6038
SacI	GAGCTC	3 cut(s)	1525, 4698, 6054
SacII	CCGCGG	1 cut(s)	6058
ScaI	AGTACT	3 cut(s)	1145, 1475, 5573
SchI	GAGTC	8 cut(s)	1523, 1720, 2919, 3453, 3619, 4755, 5895, 6467
SexAI	ACCWGGT	1 cut(s)	4651
SfcI	CTRYAG	7 cut(s)	159, 168, 1026, 1620, 1897, 5271, 6287
Sfr274I	CTCGAG	1 cut(s)	1518
Sfr303I	CCGCGG	1 cut(s)	6058
SfuI	TTCGAA	1 cut(s)	5961
SgrBI	CCGCGG	1 cut(s)	6058
SlaI	CTCGAG	1 cut(s)	1518
SmiMI	CAYNNNNRTG	9 cut(s)	465, 822, 948, 969, 2139, 2892, 4160, 5517, 6038
SmlI	CTYRAG	9 cut(s)	609, 1518, 1784, 2633, 3645, 4084, 4682, 6143, 6732
SmoI	CTYRAG	9 cut(s)	609, 1518, 1784, 2633, 3645, 4084, 4682, 6143, 6732
SnaBI	TACGTA	2 cut(s)	127, 263
SpeI	ACTAGT	1 cut(s)	1442
SseBI	AGGCCT	1 cut(s)	1696
SstI	GAGCTC	3 cut(s)	1525, 4698, 6054
StuI	AGGCCT	1 cut(s)	1696
TaiI	ACGT	9 cut(s)	129, 265, 1331, 4183, 4285, 4380, 4561, 5041, 6374
TaqII	GACCGA	1 cut(s)	4566
TatI	WGTACW	9 cut(s)	1122, 1143, 1473, 2365, 3927, 4426, 4489, 5440, 5571
TauI	GCSGC	2 cut(s)	1067, 1757
TspGWI	ACGGA	1 cut(s)	252
Van91I	CCANNNNNTGG	2 cut(s)	641, 1427
VneI	GTGCAC	2 cut(s)	3183, 3495
VspI	ATTAAT	2 cut(s)	602, 4194
XagI	CCTNNNNNAGG	1 cut(s)	3296
XceI	RCATGY	5 cut(s)	464, 653, 714, 1499, 1619
XcmI	CCANNNNNNNNNTGG	5 cut(s)	194, 1194, 1235, 4298, 5402
XhoI	CTCGAG	1 cut(s)	1518
XmiI	GTMKAC	8 cut(s)	2943, 3613, 3703, 3896, 3939, 4473, 4802, 6330
XmnI	GAANNNNTTC	2 cut(s)	21, 3088
ZraI	GACGTC	1 cut(s)	1329
ZrmI	AGTACT	3 cut(s)	1145, 1475, 5573
Zsp2I	ATGCAT	5 cut(s)	496, 523, 3579, 4317, 4894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.