Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Forward (+)

54270415 .. 54284478

Gene Structure 14064 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG458000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6807 bp

ATGTCGGAAGCTCAAAGGAGATTGTTGACAACTGTTAGTCTTCCTCGTAATAATGGTTACGTAAATGGGGGACTATCAATCAGGTCCCCTGCTGCAGCACCTGCAGTTGAGGAGTTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATAGCAAACAATGGAATGGCAGCAGTCAAGTTTATACGTAGTGTGAGGACGTGGGCTTATGAAACATTTGGTACAGAAAAGGCTGTTTTGTTGGTGGCCATGGCCACACCAGAAGACATGAGAATCAATGCAGAACACATTAGAATTGCTGATCAGTTTGTGGAAGTTCCTGGTGGAACTAACAATAATAACTATGCCAATGTGCAACTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCATCTGAGATCCCTGAGCTTCCAGATGCATTGACTGCAAAGGGCATCATATTTCTCGGTCCACCAGCTGTATCTATGGCAGCATTAGGAGATAAAATTGGTTCATCATTAATTGCTCAAGCTGCTGAAGTACCCACCCTTCCATGGAGTGGATCACATGTGAAAATTGCTTCAGACAGCTGCCTGGTTACGATCCCAGATGAAATGTATCGGGAAGCATGTGTTTACACAACAGAGGAAGCGATTGCAAGTTGTCAGGTTGTTGGTTACCCAGCCATGATTAAGGCATCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAAGTTAGGGCATTGTTCAAGCAAGTCCAGGGTGAAGTTCCTGGATCTCCCATATTCATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAAGTCCAGTTACTATGTGATCAGCATGGAAATGTTGCAGCTTTGCACAGCCGTGATTGCAGTGTTCAAAGGAGGCACCAGAAGATTATTGAGGAGGGTCCAATAACTGTAGCTCCACCAGAGACAATTAAAAAGTTGGAGCAGGCGGCTCGAAGGTTGGCTAAAAGTGTTAACTATGTTGGAGCAGCTACTGTTGAGTATCTGTACAGTATGGACACTGGCGAGTACTATTTCTTAGAGTTGAACCCTCGGTTGCAGGTGGAGCACCCAGTCACTGAGTGGATAGCAGAAATAAATCTGCCAGCAGCCCAAGTTGCAGTTGGGATGGGCATTCCCCTCTGGCAAATTCCTGAGATAAGGCGGTTCTATGGAATGGAACATGGTAGCGGATATGATGCTTGGAGAAAGACGTCAGTTGTTGCCACCCCATTTGACTTTGACAAGGCAGAATCTACAAGGCCAAAAGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAACCTACTAGTGGGAAAGTTCAGGAGCTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCGTACTTTTCTGTTAAGTCTGGAGGGGGGATTCATGAGTACTCAGATTCTCAATTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTGGATTATTCAATAGATCTTTTACATGCTTCAGATTACAGAGAAAATAAAATCCACACAGGTTGGTTGGATAGTAGAATTGCTATGCGAGTTAGAACAGAAAGGCCTCCTTGGTATTTGTCTGTAATTGGAGGGACTCTTTCTAAAGCATCTGCTAGTAGTGCGGCAATGGTTTCAGATTATATCGGCTATCTTGAGAAAGGGCAAATCCCACCTAAGCATATATCATTTGTCCATTCACAAGTGTCTTTGAACATTGAGGGGAGCAAATACACGATTGACATGGTGAGAGGGGGTCCTGGGAGCTATAGATTGCGGATGAATGAGTCAGAGGTTGAAGCAGAGATACATACTTTACGCGATGGAGGATTACTGATGCAGTTGGATGGAAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGTCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGATCACGATCCATCCAAGTTAATAGCAGAGACACCATGCAAGCTGTTGAGGTTCTTGGTTGCTGATGATAGTCATATCGATGCTGATACACCATATGCAGAGGTTGAGGTTATGAAGATGTGTATGCCTCTACTTTCACCTGCTTCTGGAGTTATCCACTTTCAATTGTCTGAAGGCCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCAGCTGTGAGAAAGACAGAACCTTTCCGTGGAAGCTTCCCTGTCCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTTCATCAAAGATGTGCTGCAAGCTTAAACGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCAATTGCCTAGATAGTCCTGAACTCCCTTTCCTTCAATGGCAAGAATGCTTGGCTGTTTTGGCAACCCGCCTACCCAAGAATCTCAAAAATGAACTGGAATCAAAGTTTAAGGATTTTGAGTTGATTTCTAGCTCTCAGAATGTTGATTTCCCTGCCAAATTGTTGCGTGGTGTTCTTGAGGCCCATCTATTCTCCTGTCCTGATAAAGAAAAAGGAGCCCAAGAAAGGCTTGTTGAACCTTTGATGAGCCTTGTGACGTCTTATGAAGGTGGAAGAGAAAGTCATGCCCGAGTTATTGTTCAATCCCTCTTTGAAGAGTATCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAGCGCCTTCGACTTCAATATAAGAAAGATCTGTTGAAGGTTGTGGAAATTGTGCTCTCTCATCAGGGTGTAAAGAATAAAAATAAGTTGATTCTACGACTCATGGAACAACTGGTGTACCCCAACCCTGCTGCATACAGGGAGAAGCTAATTCGGTTTTCTCAACTTAACCATACAAACTATTCTCAGTTGGCACTAAAAGCAAGTCAGCTTATGGAGCAAACCAAATTGAGTGAACTTCGTTCTAGCATTGCTAGAAGTCTTTCTGAGTTGGAGATGTTTACTGAGGATGGTGAAACCATGGATACTCCAAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCACCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTCTTTGATCATGGTGATCACACCCTTCAGAGGCGGGTTGTCGAGAGTTATGTACGCCGGTTATACCAGCCCTACCTTGTAAAGGGGAGTGTCAGGATGCAGTGGCACAGATCTGGTCTAATAGCTTCATGGGAGTTCTCGGAAGAACCTGTTGAGAGGAAACATGAGATTGAAGGTCAATCAGTTGATAAAACATTCCTTCAGAAACACGAGGAGAGGAAATGGGGAGTCATGGTTATAATAAAATCTCTTCAGTTTCTTCCAGCAATTATTAGTGGCGCACTGAAGGAAATGTCTCATAACCTTCATGAAGCAACTCCAAATGGATCTACTGAACCAAGTGGCTTTGGTAATATGATGCATATTGCTTTGGTTGGCATCAACAATCAGATGAGTCTACTTCAGGATAGTGGTGACGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATACTTAAAGAGCAAGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCACAGTAATTAGTTGTATCATACAAAGGGATGAAGGGCGGGCCCCTATGAGGCACTCCTTCCACTGGTCATCAGAGAAGCTCTATTATGAAGAAGAACCCCTATTGCGTCACCTAGAACCTCCTCTATCTATTTACCTTGAATTGGACAAGCTTAAAGGCTATGAGAATATACAGTATACCCCATCTCGTGACCGTCAATGGCACTTGTACACCGTTGTAGACAAGCCACTACCAATCCAAAGGATGTTCCTCAGAACACTTGTCAGGCAGCCCACAACAAATGAAGGGTTCTCTGGATTTCAACGGCTAGACATTGCAACAGCTAGTAACCAATTTTCTTTATCATTTACTTCGAGGAGCATCTTGAGGTCCTTATCAACAGCAATGGAAGAGTTGGAACTGAATTCGCACAATGCCACTGTCAAATCTGACCACACCCATATGTATCTTTATATCTTACGTGAGCAACAAATTAATGATCTGCTGCCTTACCACAAGAGAGTTGATTTAGATGCTCAACAAGAAGAAACTGTTGTGGAGGCCATCTTGGAAGAACTTGCACGTGAAATCCATACATCAGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGCTGTGGATGGCATCCTCTGGAGAGGCAAACGTTGCTTGGAGGGTTGTCGTGACAAATGTGACTGGTCATACCTGCACTGTACATATATATCGGGAACTAGAAGATACCAGCAAACAGGGAGCGGTATACCATTCAGTCTCTGTACAGGGTCCCCTGCATGGTGTACCAGTGAATGCGCACTATCAGCCTTTGGGAATTATAGACCGAAAACGTCTGTTGGCCAGGAGAACCAACACCACTTACTGCTATGATTTTCCACTGGCATTTGAGACGGCCTTGGAACAGTCATGGGCATCCCAGTCACCAGGTGTTGACAAACCGAAGGGTAAAATTCTCAAGGTCACAGAGCTCAAATTCGCTGACCAAAAAGGCAGCTGGGGTACTCCTCTCAATACCGTAGAGCGTCCACCTGGACTCAATGATGTTGGTATGATAGCCTGGTTTATGGAGATGTCTACCCCTGAGTTCCCTTCTGGAAGGAGGATATTTGTTGTTGCAAATGATGTCACCTACAAAGCTGGATCTTTTGGCCCAAGGGAGGATGCATTCTTCTTTGCAGTAACTGAGCTTGCTTGTGCTGAGAAATTGCCTCTAATATACTTGGCAGCAAACTCAGGTGCCCGTATTGGGGTTGCTGAAGAAGTCAAATCCTGTTTTAAAGTGGGCTGGTCTGATGAATCAAGCCCCGAACGTGGTTTTCAATATGTGTATTTAACCTCTGAGGATTATGCTCGGATTGGATCATCTGTGATAGCGCATGAATTAAACCTAGCAAATGGCGAAACCAGATGGGTGATAGATACCATTGTTGGAAAGGAGGACGGCTTGGGGGTCGAGAGCTTGACTGGAAGTGGGGCAATTGCTGGTGCATATTCGAGGGCATATATGGAAACCTTTACCTTAACATATGTGACGGGCAGAACTGTAGGGATAGGTGCTTATCTTGCAAGACTTGGGATGCGGTGCATCCAGAGGCTTGATCAGCCCATCATTTTAACTGGTTTCTCTGCATTAAACAAACTACTTGGCCGGGAGGTTTACAGCTCCCATATGCAGCTTGGAGGACCTAAAATTATGGGAACTAATGGGGTTGTACATCTAACGGTAGCTGATGATCTTGAAGGAATATCTGCTATTCTAAAGTGGCTGAGCTATGTTCCTCCCCATGTTGGTGGTCCACTCCCCATTTCAATTCCCTTGGATCCTCCAGAAAGACGTGTGGAGTACTGCCCCGAGAATTCATGTGATCCTCGTGCTGCGATTTCTGGTGCTCTGAATGGTAATGGGAACTGGATGGGGGGTATATTTGACAAAGACAGCTTTGTTGAGACGCTAGAAGGCTGGGCAAGGACGGTTGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTGGGAATTGTTGCTGTTGAGACACAGACGGTGATGCAAATTATTCCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGACAAGTATGGTTTCCTGATTCTGCAACTAAGACTGCCCAAGCATTATTAGATTTCAACAGAGAAGGACTCCCACTTTTCATTCTTGCCAACTGGAGAGGCTTTTCTGGTGGGCAGAGAGACCTTTTCGAAGGGATCCTTCAGGCTGGATCAACCATTGTGGAGAACCTAAGGACTTACAAACAGCCCATTTTTGTATTCATCCCAATGATGGGTGAGCTCCGCGGTGGAGCATGGGTGGTCGTTGATAGCCGGATAAATCCGGACCATATTGAAATGTATGCTGATCGAACGGCTAGAGGAAATGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTTAGGAACAAGGAATTGCTGGAGTGCATGGGTAGGCTTGACCAACAGCTGATCCAACTAAAGACTAGGCTTCAGGAAGCCAGGAGCTGTCGGGTGAATGAGAATGTTGAATCCCTACAGTTTCAGATACGATCCCGAGAAAAACAGCTTTTGCCCGTCTACACTCAGATAGCTACCAAATTTGCAGAACTTCATGATACGTCTCTGAGGATGGCTGCAAAAGGGGTAATCAGAGGAGTTTTGGAGTGGGCCAGCTCTCGATCTTTCTTCTACAAGAGATTGTGTCGGAGAATTGCCGATGACTCACTCATCAAGATAGTGAGAGATGCTGCTGGTGAACAGCTGTCACATAGATCTGCAATGGATTTGATCAAGGGCTGGTTTTTGAGTTCTGATATTTCAAGAGGCAGGGAAAATGCTTGGGAGGATGATGAAACTTTCTTTAGATGGAAGGATGATCAAACTAATTATGAGGGTAAACTAAAAGAATTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAACCATTGGCAACTCAGCATCTGATTTGCAAGCCCTACCTCAAGGTCTTGCTGCCCTTCTAAGCAAGGTTGAGCCATCAAGCAGATCATTGTTGGTTGAGGAACTCCGGAAGGTGCTTGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

2268

Amino Acids

252.87

Weight (kDa)

5.98

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	49 - 168	5.1e-28	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	216 - 398	5.1e-46	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	217 - 368	2.6e-07	ATP-grasp domain
ATP-grasp_3	PF02655	223 - 370	2.3e-07	ATP-grasp domain
Biotin_carb_C	PF02785	445 - 551	1.1e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	567 - 682	3.3e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	693 - 755	1e-08	Biotin-requiring enzyme
ACC_central	PF08326	756 - 1499	3.6e-178	Acetyl-CoA carboxylase, central region
Carboxyl_trans	PF01039	1601 - 2151	4.1e-148	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3446
AarI	CACCTGC	4 cut(s)	109, 1099, 2200, 3695
AatII	GACGTC	2 cut(s)	1265, 2708
Acc16I	TGCGCA	1 cut(s)	4515
Acc36I	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
AccB1I	GGYRCC	2 cut(s)	927, 4955
AccB7I	CCANNNNNTGG	2 cut(s)	575, 1361
AccBSI	CCGCTC	1 cut(s)	4460
AccI	GTMKAC	7 cut(s)	3604, 3694, 3887, 3930, 4464, 4793, 6321
AccII	CGCG	2 cut(s)	1941, 6048
AccIII	TCCGGA	2 cut(s)	6085, 6789
AclI	AACGTT	1 cut(s)	4368
AcoI	YGGCCR	4 cut(s)	255, 261, 4557, 5365
AcsI	RAATTY	9 cut(s)	1197, 1551, 1557, 1569, 4114, 4668, 4691, 5575, 6341
AcvI	CACGTG	1 cut(s)	4274
AcyI	GRCGYC	2 cut(s)	1262, 2705
AdeI	CACNNNGTG	2 cut(s)	1131, 4646
AflIII	ACRYGT	4 cut(s)	394, 583, 1486, 5554
AhlI	ACTAGT	1 cut(s)	1376
AjiI	CACGTC	2 cut(s)	210, 5555
AjuI	GAANNNNNNNTTGG	2 cut(s)	2329, 2361
AleI	CACNNNNGTG	1 cut(s)	903
AloI	GAACNNNNNNTCC	2 cut(s)	2460, 2492
Alw21I	GWGCWC	7 cut(s)	1119, 1516, 2874, 3178, 4689, 5611, 6045
Alw44I	GTGCAC	1 cut(s)	3174
AlwNI	CAGNNNCTG	8 cut(s)	101, 1043, 1127, 1396, 4478, 4901, 6249, 6704
Ama87I	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
Aor13HI	TCCGGA	2 cut(s)	6085, 6789
ApaI	GGGCCC	1 cut(s)	3754
ApaLI	GTGCAC	1 cut(s)	3174
ApoI	RAATTY	9 cut(s)	1197, 1551, 1557, 1569, 4114, 4668, 4691, 5575, 6341
AseI	ATTAAT	2 cut(s)	536, 4185
Asp700I	GAANNNNTTC	1 cut(s)	3079
AspLEI	GCGC	4 cut(s)	2823, 3488, 4516, 5095
AsuC2I	CCSGG	1 cut(s)	5369
AsuII	TTCGAA	1 cut(s)	5952
AvaI	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
AxyI	CCTNAGG	2 cut(s)	5817, 5993
BaeGI	GKGCMC	3 cut(s)	3178, 3754, 4960
BaeI	ACNNNNGTAYC	4 cut(s)	2918, 2951, 5130, 5163
BalI	TGGCCA	3 cut(s)	257, 263, 4559
BamHI	GGATCC	3 cut(s)	1351, 5539, 5958
BanI	GGYRCC	2 cut(s)	927, 4955
BanII	GRGCYC	5 cut(s)	1516, 2668, 3754, 4689, 6045
BarI	GAAGNNNNNNTAC	6 cut(s)	549, 581, 846, 878, 1911, 1943
BauI	CACGAG	3 cut(s)	3416, 3897, 5589
BbrPI	CACGTG	1 cut(s)	4274
BbsI	GAAGAC	3 cut(s)	32, 279, 2009
Bbv12I	GWGCWC	7 cut(s)	1119, 1516, 2874, 3178, 4689, 5611, 6045
BceAI	ACGGC	5 cut(s)	888, 4031, 4626, 5176, 6132
BcgI	CGANNNNNNTGC	2 cut(s)	983, 1017
BciVI	GTATCC	1 cut(s)	3115
BclI	TGATCA	7 cut(s)	310, 871, 3214, 3223, 5317, 6531, 6619
BcnI	CCSGG	1 cut(s)	5369
BcuI	ACTAGT	1 cut(s)	1376
BfmI	CTRYAG	6 cut(s)	93, 102, 960, 1888, 5262, 6278
BfoI	RGCGCY	1 cut(s)	2824
BfuAI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
BfuI	GTATCC	1 cut(s)	3115
BglII	AGATCT	4 cut(s)	1597, 2845, 3317, 6515
BlpI	GCTNAGC	1 cut(s)	5486
BmcAI	AGTACT	3 cut(s)	1079, 1466, 5564
BmeT110I	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
BmgBI	CACGTC	2 cut(s)	210, 5555
BmrI	ACTGGG	2 cut(s)	1115, 4630
BmuI	ACTGGG	2 cut(s)	1115, 4630
BpiI	GAAGAC	3 cut(s)	32, 279, 2009
BplI	GAGNNNNNCTC	2 cut(s)	5877, 5909
BpmI	CTGGAG	7 cut(s)	1467, 2220, 3673, 4377, 5529, 5938, 6203
Bpu10I	CCTNAGC	2 cut(s)	441, 1797
Bpu1102I	GCTNAGC	1 cut(s)	5486
Bpu14I	TTCGAA	1 cut(s)	5952
BpuEI	CTTGAG	8 cut(s)	528, 1796, 2645, 3621, 4096, 4658, 6155, 6708
BpuMI	CCSGG	1 cut(s)	5369
Bsa29I	ATCGAT	1 cut(s)	2130
BsaAI	YACGTR	4 cut(s)	61, 197, 4172, 4274
BsaBI	GATNNNNATC	2 cut(s)	2058, 6155
BsaHI	GRCGYC	2 cut(s)	1262, 2705
BsaI	GGTCTC	2 cut(s)	3212, 5937
BsaWI	WCCGGW	2 cut(s)	6085, 6789
BsaXI	ACNNNNNCTCC	6 cut(s)	949, 979, 3426, 3456, 6045, 6075
Bse118I	RCCGGY	1 cut(s)	3264
Bse21I	CCTNAGG	2 cut(s)	5817, 5993
Bse3DI	GCAATG	6 cut(s)	1755, 2241, 3066, 4023, 4101, 6528
Bse8I	GATNNNNATC	2 cut(s)	2058, 6155
BseAI	TCCGGA	2 cut(s)	6085, 6789
BseCI	ATCGAT	1 cut(s)	2130
BseJI	GATNNNNATC	2 cut(s)	2058, 6155
BseMI	GCAATG	6 cut(s)	1755, 2241, 3066, 4023, 4101, 6528
BseRI	GAGGAG	8 cut(s)	125, 959, 1578, 3434, 3822, 4081, 4713, 6411
BseSI	GKGCMC	3 cut(s)	3178, 3754, 4960
BseYI	CCCAGC	3 cut(s)	697, 4713, 5679
BsgI	GTGCAG	2 cut(s)	3723, 4396
Bsh1236I	CGCG	2 cut(s)	1941, 6048
BshNI	GGYRCC	2 cut(s)	927, 4955
BshVI	ATCGAT	1 cut(s)	2130
BsiHKAI	GWGCWC	7 cut(s)	1119, 1516, 2874, 3178, 4689, 5611, 6045
BsiHKCI	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
BsiSI	CCGG	5 cut(s)	3265, 5368, 6076, 6086, 6790
BslFI	GGGAC	4 cut(s)	70, 84, 1729, 4473
BsmBI	CGTCTC	3 cut(s)	4601, 5660, 6369
BsmFI	GGGAC	4 cut(s)	70, 84, 1729, 4473
BsmI	GAATGC	4 cut(s)	1182, 2498, 4516, 4883
Bso31I	GGTCTC	2 cut(s)	3212, 5937
BsoBI	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
Bsp119I	TTCGAA	1 cut(s)	5952
Bsp120I	GGGCCC	1 cut(s)	3750
Bsp13I	TCCGGA	2 cut(s)	6085, 6789
Bsp1407I	TGTACA	5 cut(s)	1056, 3918, 4417, 4480, 5431
Bsp1720I	GCTNAGC	1 cut(s)	5486
Bsp19I	CCATGG	3 cut(s)	258, 569, 3117
BspDI	ATCGAT	1 cut(s)	2130
BspEI	TCCGGA	2 cut(s)	6085, 6789
BspFNI	CGCG	2 cut(s)	1941, 6048
BspHI	TCATGA	3 cut(s)	1459, 3514, 6355
BspMAI	CTGCAG	2 cut(s)	97, 106
BspMI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
BspT104I	TTCGAA	1 cut(s)	5952
BspT107I	GGYRCC	2 cut(s)	927, 4955
BspTNI	GGTCTC	2 cut(s)	3212, 5937
BsrBI	CCGCTC	1 cut(s)	4460
BsrDI	GCAATG	6 cut(s)	1755, 2241, 3066, 4023, 4101, 6528
BsrFI	RCCGGY	1 cut(s)	3264
BsrGI	TGTACA	5 cut(s)	1056, 3918, 4417, 4480, 5431
BssAI	RCCGGY	1 cut(s)	3264
BssNAI	GTATAC	2 cut(s)	3888, 4465
BssNI	GRCGYC	2 cut(s)	1262, 2705
BssSI	CACGAG	3 cut(s)	3416, 3897, 5589
BssT1I	CCWWGG	8 cut(s)	127, 258, 569, 1691, 3117, 4614, 4871, 5535
Bst1107I	GTATAC	2 cut(s)	3888, 4465
Bst2BI	CACGAG	3 cut(s)	3416, 3897, 5589
Bst6I	CTCTTC	4 cut(s)	2716, 2757, 3462, 4095
BstACI	GRCGYC	2 cut(s)	1262, 2705
BstAPI	GCANNNNNTGC	4 cut(s)	101, 461, 2443, 4370
BstAUI	TGTACA	5 cut(s)	1056, 3918, 4417, 4480, 5431
BstBAI	YACGTR	4 cut(s)	61, 197, 4172, 4274
BstBI	TTCGAA	1 cut(s)	5952
BstDSI	CCRYGG	5 cut(s)	258, 569, 2308, 3117, 6046
BstEII	GGTNACC	1 cut(s)	692
BstENI	CCTNNNNNAGG	1 cut(s)	3287
BstFNI	CGCG	2 cut(s)	1941, 6048
BstH2I	RGCGCY	1 cut(s)	2824
BstHHI	GCGC	4 cut(s)	2823, 3488, 4516, 5095
BstNSI	RCATGY	5 cut(s)	398, 587, 648, 1490, 1610
BstPI	GGTNACC	1 cut(s)	692
BstSFI	CTRYAG	6 cut(s)	93, 102, 960, 1888, 5262, 6278
BstSLI	GKGCMC	3 cut(s)	3178, 3754, 4960
BstSNI	TACGTA	2 cut(s)	61, 197
BstUI	CGCG	2 cut(s)	1941, 6048
BstV2I	GAAGAC	3 cut(s)	32, 279, 2009
BstXI	CCANNNNNNTGG	2 cut(s)	1422, 5510
BstZ17I	GTATAC	2 cut(s)	3888, 4465
Bsu15I	ATCGAT	1 cut(s)	2130
Bsu36I	CCTNAGG	2 cut(s)	5817, 5993
BsuI	GTATCC	1 cut(s)	3115
BsuTUI	ATCGAT	1 cut(s)	2130
BtgI	CCRYGG	5 cut(s)	258, 569, 2308, 3117, 6046
BtgZI	GCGATG	1 cut(s)	1956
BtrI	CACGTC	2 cut(s)	210, 5555
BtsI	GCAGTG	2 cut(s)	919, 3314
BveI	ACCTGC	5 cut(s)	109, 1099, 2200, 3695, 4418
CaiI	CAGNNNCTG	8 cut(s)	101, 1043, 1127, 1396, 4478, 4901, 6249, 6704
CciI	TCATGA	3 cut(s)	1459, 3514, 6355
CfoI	GCGC	4 cut(s)	2823, 3488, 4516, 5095
Cfr10I	RCCGGY	1 cut(s)	3264
Cfr42I	CCGCGG	1 cut(s)	6049
ClaI	ATCGAT	1 cut(s)	2130
CseI	GACGC	3 cut(s)	3806, 4730, 5677
CsiI	ACCWGGT	1 cut(s)	4642
CspCI	CAANNNNNGTGG	6 cut(s)	1264, 1299, 1625, 1660, 5964, 5999
DraI	TTTAAA	3 cut(s)	1369, 1405, 4996
DraIII	CACNNNGTG	2 cut(s)	1131, 4646
EaeI	YGGCCR	4 cut(s)	255, 261, 4557, 5365
Eam1104I	CTCTTC	4 cut(s)	2716, 2757, 3462, 4095
EarI	CTCTTC	4 cut(s)	2716, 2757, 3462, 4095
Ecl136II	GAGCTC	3 cut(s)	1514, 4687, 6043
Eco105I	TACGTA	2 cut(s)	61, 197
Eco130I	CCWWGG	8 cut(s)	127, 258, 569, 1691, 3117, 4614, 4871, 5535
Eco147I	AGGCCT	1 cut(s)	1687
Eco24I	GRGCYC	5 cut(s)	1516, 2668, 3754, 4689, 6045
Eco31I	GGTCTC	2 cut(s)	3212, 5937
Eco53kI	GAGCTC	3 cut(s)	1514, 4687, 6043
Eco72I	CACGTG	1 cut(s)	4274
Eco81I	CCTNAGG	2 cut(s)	5817, 5993
Eco88I	CYCGRG	4 cut(s)	1509, 2736, 5570, 6297
Eco91I	GGTNACC	1 cut(s)	692
EcoICRI	GAGCTC	3 cut(s)	1514, 4687, 6043
EcoNI	CCTNNNNNAGG	1 cut(s)	3287
EcoO109I	RGGNCCY	7 cut(s)	84, 1877, 2330, 3751, 4080, 4487, 5402
EcoO65I	GGTNACC	1 cut(s)	692
EcoRI	GAATTC	2 cut(s)	4114, 5575
EcoT14I	CCWWGG	8 cut(s)	127, 258, 569, 1691, 3117, 4614, 4871, 5535
EcoT22I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
EcoT38I	GRGCYC	5 cut(s)	1516, 2668, 3754, 4689, 6045
ErhI	CCWWGG	8 cut(s)	127, 258, 569, 1691, 3117, 4614, 4871, 5535
Esp3I	CGTCTC	3 cut(s)	4601, 5660, 6369
FalI	AAGNNNNNCTT	8 cut(s)	1675, 1707, 2661, 2693, 3183, 3215, 5946, 5978
FaqI	GGGAC	4 cut(s)	70, 84, 1729, 4473
FauI	CCCGC	4 cut(s)	2522, 3234, 3741, 6648
FauNDI	CATATG	4 cut(s)	2146, 4152, 5245, 5388
FbaI	TGATCA	7 cut(s)	310, 871, 3214, 3223, 5317, 6531, 6619
FblI	GTMKAC	7 cut(s)	3604, 3694, 3887, 3930, 4464, 4793, 6321
FriOI	GRGCYC	5 cut(s)	1516, 2668, 3754, 4689, 6045
FspAI	RTGCGCAY	1 cut(s)	4515
FspI	TGCGCA	1 cut(s)	4515
GlaI	GCGC	4 cut(s)	2822, 3487, 4515, 5094
GsaI	CCCAGC	3 cut(s)	701, 4717, 5683
GsuI	CTGGAG	7 cut(s)	1467, 2220, 3673, 4377, 5529, 5938, 6203
HaeII	RGCGCY	1 cut(s)	2824
HapII	CCGG	5 cut(s)	3265, 5368, 6076, 6086, 6790
HgaI	GACGC	3 cut(s)	3806, 4730, 5677
HhaI	GCGC	4 cut(s)	2823, 3488, 4516, 5095
Hin1I	GRCGYC	2 cut(s)	1262, 2705
Hin6I	GCGC	4 cut(s)	2821, 3486, 4514, 5093
HinP1I	GCGC	4 cut(s)	2821, 3486, 4514, 5093
HincII	GTYRAC	3 cut(s)	27, 1024, 4651
HindII	GTYRAC	3 cut(s)	27, 1024, 4651
HindIII	AAGCTT	4 cut(s)	2314, 2377, 3860, 5711
HpaI	GTTAAC	1 cut(s)	1024
HpaII	CCGG	5 cut(s)	3265, 5368, 6076, 6086, 6790
Hsp92I	GRCGYC	2 cut(s)	1262, 2705
HspAI	GCGC	4 cut(s)	2821, 3486, 4514, 5093
KflI	GGGWCCC	1 cut(s)	4487
Kpn2I	TCCGGA	2 cut(s)	6085, 6789
Ksp22I	TGATCA	7 cut(s)	310, 871, 3214, 3223, 5317, 6531, 6619
KspAI	GTTAAC	1 cut(s)	1024
KspI	CCGCGG	1 cut(s)	6049
MabI	ACCWGGT	1 cut(s)	4642
MbiI	CCGCTC	1 cut(s)	4460
MfeI	CAATTG	4 cut(s)	2216, 2449, 5196, 6677
MlsI	TGGCCA	3 cut(s)	257, 263, 4559
MluNI	TGGCCA	3 cut(s)	257, 263, 4559
MlyI	GAGTC	9 cut(s)	1514, 1711, 1916, 2910, 3444, 3610, 4746, 5886, 6458
Mox20I	TGGCCA	3 cut(s)	257, 263, 4559
Mph1103I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
MroI	TCCGGA	2 cut(s)	6085, 6789
MroXI	GAANNNNTTC	1 cut(s)	3079
MscI	TGGCCA	3 cut(s)	257, 263, 4559
MslI	CAYNNNNRTG	9 cut(s)	399, 756, 882, 903, 2130, 2883, 4151, 5508, 6029
Msp20I	TGGCCA	3 cut(s)	257, 263, 4559
MspA1I	CMGCKG	8 cut(s)	494, 606, 2006, 2285, 4713, 6048, 6211, 6505
MspI	CCGG	5 cut(s)	3265, 5368, 6076, 6086, 6790
MunI	CAATTG	4 cut(s)	2216, 2449, 5196, 6677
Mva1269I	GAATGC	4 cut(s)	1182, 2498, 4516, 4883
MvnI	CGCG	2 cut(s)	1941, 6048
NciI	CCSGG	1 cut(s)	5369
NcoI	CCATGG	3 cut(s)	258, 569, 3117
NdeI	CATATG	4 cut(s)	2146, 4152, 5245, 5388
NsbI	TGCGCA	1 cut(s)	4515
NsiI	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885
NspI	RCATGY	5 cut(s)	398, 587, 648, 1490, 1610
NspV	TTCGAA	1 cut(s)	5952
OliI	CACNNNNGTG	1 cut(s)	903
PaeR7I	CTCGAG	1 cut(s)	1509
PagI	TCATGA	3 cut(s)	1459, 3514, 6355
PaqCI	CACCTGC	4 cut(s)	109, 1099, 2200, 3695
PceI	AGGCCT	1 cut(s)	1687
PciI	ACATGT	3 cut(s)	394, 583, 1486
PctI	GAATGC	4 cut(s)	1182, 2498, 4516, 4883
PdmI	GAANNNNTTC	1 cut(s)	3079
PflMI	CCANNNNNTGG	2 cut(s)	575, 1361
PfoI	TCCNGGA	1 cut(s)	802
PleI	GAGTC	9 cut(s)	1513, 1711, 1915, 2910, 3443, 3609, 4746, 5886, 6458
PmaCI	CACGTG	1 cut(s)	4274
PmlI	CACGTG	1 cut(s)	4274
PpsI	GAGTC	9 cut(s)	1513, 1711, 1915, 2910, 3443, 3609, 4746, 5886, 6458
Ppu21I	YACGTR	4 cut(s)	61, 197, 4172, 4274
PpuMI	RGGWCCY	5 cut(s)	84, 1877, 4080, 4487, 5402
PscI	ACATGT	3 cut(s)	394, 583, 1486
PshBI	ATTAAT	2 cut(s)	536, 4185
PsiI	TTATAA	1 cut(s)	3446
Psp124BI	GAGCTC	3 cut(s)	1516, 4689, 6045
Psp1406I	AACGTT	1 cut(s)	4368
Psp5II	RGGWCCY	5 cut(s)	84, 1877, 4080, 4487, 5402
PspCI	CACGTG	1 cut(s)	4274
PspEI	GGTNACC	1 cut(s)	692
PspFI	CCCAGC	3 cut(s)	697, 4713, 5679
PspOMI	GGGCCC	1 cut(s)	3750
PspPPI	RGGWCCY	5 cut(s)	84, 1877, 4080, 4487, 5402
PstI	CTGCAG	2 cut(s)	97, 106
PstNI	CAGNNNCTG	8 cut(s)	101, 1043, 1127, 1396, 4478, 4901, 6249, 6704
PvuII	CAGCTG	7 cut(s)	494, 606, 2006, 2285, 4713, 6211, 6505
RseI	CAYNNNNRTG	9 cut(s)	399, 756, 882, 903, 2130, 2883, 4151, 5508, 6029
SacI	GAGCTC	3 cut(s)	1516, 4689, 6045
SacII	CCGCGG	1 cut(s)	6049
ScaI	AGTACT	3 cut(s)	1079, 1466, 5564
SchI	GAGTC	9 cut(s)	1514, 1711, 1916, 2910, 3444, 3610, 4746, 5886, 6458
SexAI	ACCWGGT	1 cut(s)	4642
SfcI	CTRYAG	6 cut(s)	93, 102, 960, 1888, 5262, 6278
Sfr274I	CTCGAG	1 cut(s)	1509
Sfr303I	CCGCGG	1 cut(s)	6049
SfuI	TTCGAA	1 cut(s)	5952
SgrBI	CCGCGG	1 cut(s)	6049
SlaI	CTCGAG	1 cut(s)	1509
SmiMI	CAYNNNNRTG	9 cut(s)	399, 756, 882, 903, 2130, 2883, 4151, 5508, 6029
SmlI	CTYRAG	9 cut(s)	543, 1509, 1775, 2624, 3636, 4075, 4673, 6134, 6723
SmoI	CTYRAG	9 cut(s)	543, 1509, 1775, 2624, 3636, 4075, 4673, 6134, 6723
SnaBI	TACGTA	2 cut(s)	61, 197
SpeI	ACTAGT	1 cut(s)	1376
SseBI	AGGCCT	1 cut(s)	1687
SstI	GAGCTC	3 cut(s)	1516, 4689, 6045
StuI	AGGCCT	1 cut(s)	1687
StyI	CCWWGG	8 cut(s)	127, 258, 569, 1691, 3117, 4614, 4871, 5535
TaqII	GACCGA	2 cut(s)	473, 4557
TatI	WGTACW	8 cut(s)	1056, 1077, 1464, 3918, 4417, 4480, 5431, 5562
TauI	GCSGC	2 cut(s)	1001, 1748
TspGWI	ACGGA	1 cut(s)	2297
Van91I	CCANNNNNTGG	2 cut(s)	575, 1361
VneI	GTGCAC	1 cut(s)	3174
VspI	ATTAAT	2 cut(s)	536, 4185
XagI	CCTNNNNNAGG	1 cut(s)	3287
XapI	RAATTY	9 cut(s)	1197, 1551, 1557, 1569, 4114, 4668, 4691, 5575, 6341
XceI	RCATGY	5 cut(s)	398, 587, 648, 1490, 1610
XcmI	CCANNNNNNNNNTGG	4 cut(s)	1128, 1169, 4289, 5393
XhoI	CTCGAG	1 cut(s)	1509
XmiI	GTMKAC	7 cut(s)	3604, 3694, 3887, 3930, 4464, 4793, 6321
XmnI	GAANNNNTTC	1 cut(s)	3079
ZraI	GACGTC	2 cut(s)	1263, 2706
ZrmI	AGTACT	3 cut(s)	1079, 1466, 5564
Zsp2I	ATGCAT	5 cut(s)	430, 457, 3570, 4308, 4885

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.