RchiOBHm_Chr7g0239931

Acetyl-CoA Carboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001986
Physical Location & Seq
Forward (+)
65727424 .. 65741215
13792 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21506

Sequence Viewer

Length: 6816 bp
ATGTCGGAAGCTCAAAGGAGATTGTTGACAACTGTTAGTCTTCCTCGTAATAATGGTTACGTAAATGGGGGACTATCAATCAGGTCCCCTGCTGCAGCACCTGCAGTTGAGGAGTTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATAGCAAACAATGGAATGGCAGCAGTCAAGTTTATACGTAGTGTGAGGACGTGGGCTTATGAAACATTTGGTACAGAAAAGGCTGTTTTGTTGGTGGCCATGGCCACACCAGAAGACATGAGAATCAATGCAGAACACATTAGAATTGCTGATCAGTTTGTGGAAGTTCCTGGTGGAACTAACAATAATAACTATGCCAATGTGCAACTCATTGTAGAGATGGCAGAGATAACACATGTTGATGCAGTTTGGCCTGGTTGGGGCCATGCATCTGAGATCCCTGAGCTTCCAGATGCATTGACTGCAAAGGGCATCATATTTCTCGGGCCACCAGCTGTATCTATGGCAGCATTAGGAGATAAAATTGGTTCATCATTAATTGCTCAAGCTGCTGAAGTACCCACCCTTCCATGGAGTGGATCACATGTGAAAATTGCTTCAGACAGCTGCCTGGTTACAATCCCAGATGAAATGTATCGAGAAGCATGTGTTTACACAACAGAGGAAGCGATTGCAAGTTGTCAGGTTGTTGGTTACCCAGCCATGATTAAGGCATCATGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAAGTTAGGGCATTGTTCAAGCAAGTCCAGGGTGAAGTTCCTGGATCTCCCATATTCATAATGAAAGTCGCATCCCAGAGCCGACATTTAGAAGTCCAGTTACTATGTGATCAGCATGGAAATGTTGCAGCTTTGCACAGCCGTGATTGCAGTGTTCAAAGGAGGCACCAGAAGATTATTGAGGAGGGTCCAATAACTGTAGCTCCACCAGAGACAATTAAAAAGTTGGAGCAGGCGGCTCGAAGGTTGGCTAAAAGTGTTAACTATGTTGGAGCAGCTACTGTTGAGTATCTGTACAGTATGGACACTGGCGAGTACTATTTCTTAGAGTTGAACCCTCGGTTGCAGGTGGAGCACCCAGTCACTGAGTGGATAGCAGAAATAAATCTGCCAGCAGCCCAAGTTGCAGTTGGGATGGGCATTCCTCTCTGGCAAATTCCTGAGATAAGGCGGTTCTATGGAATGGAACATGGTAGTGGATATGATGCTTGGAGAAAGACGTCAGCTGTTGCCACCCCATTTGACTTTGACAAGGCAGAATCTACAAGGCCAAAAGGTCATTGTGTTGCTGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAACCTACTAGTGGGAAAGTTCAGGAGCTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCGTACTTTTCTGTTAAGTCTGGAGGGGGGATTCATGAGTACTCAGATTCCCAGTTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTCGAGGAGAAATTCGTACAAATGTGGATTATTCAATAGATCTTTTACATGCTTCAGATTACAGAGAAAATAAAATCCACACAGGTTGGTTGGATAGTAGAATTGCTATGCGAGTTAGAACAGAAAGGCCTCCTTGGTATTTGTCTGTAATTGGAGGGACTCTTTTTAAAGCATCTGCTAGTAGTGCGGCGATGGTTTCAGATTATATCGGCTATCTTGAGAAAGGGCAAATCCCACCTAAGCATATATCATTTGTCCATTCACAAGTGTCTTTGAACATTGAGGGGAGCAAATACACGATTGACATGGTGAGAGGGGGTCCTGGGAGCTATAGATTGCGGATGAATGAGTCAGAGGTTGAAGCAGAGATACATACTTTACGCGATGGAGGATTACTGATGCAGTTGGATGGAAATAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGTCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAACGATCACGATCCATCCAAGTTAATAGCAGAGACACCATGCAAGCTGTTGAGGTTCTTGGTTGCTGATGATAGTCATATCGATGCTGATACACCATATGCAGAGGTTGAGGTTATGAAGATGTGTATGCCTCTACTTTCACCTGCTTCTGGAGTTATCCACTTTCAATTGTCTGAAGGCCAAGCAATGCAGGCTGGTGAACTTATTGCAAGGCTTGATTTAGATGATCCTTCAGCTGTGAGAAAGACAGAACCTTTCCATGGAAGCTTCCCCGTCCTGGGGCCTCCAACTGCAATTTCTGGTAAAATTCATCAAAGATGTGCTGCAAGCTTAAACGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCAATTGCCTAGATAGTCCTGAACTCCCTTTCCTTCAATGGCAAGAATGCTTGGCTGTTTTGGCAACCCGCCTACCCAAGAATCTCAAAAATGAACTGGAATCAAAATTTAAGGATTTTGAGTTGATTTCTAGCTCTCAGAGTGTTGATTTTCCTGCCAAATTGTTGCGTGGTGTTCTTGAGGCCCATCTATCCTCCTGTCCTGATAAAGATAAAGGAGCCCAAGAAAGGCTTGTTGAACCTTTGATGAGCCTTGTGACGTCTTATGAAGGTGGAAGAGAAAGTCATGCCCGAGTTATTGTTCAATCCCTCTTTGAAGAGTATCTGTCAGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAGCGCCTTCGACTTCAATATAAGAAAGATCTGTTGAAGGTTGTGGAAATTGTGCTCTCTCATCAGGGTGTAAAGAATAAAAATAAGTTGATTCTACGACTCATGGAACAACTGGTGTACCCCAACCCTGCTGCATACAGGGAGAAGCTAATTCGGTTTTCTCAACTTAACCATACAAACTATTCTCAGTTGGCACTAAAAGCAAGTCAGCTTATGGAGCAAACCAAGTTGAGTGAACTTCGTTCTAGCATTGCTAGAAGTCTTTCTGAGTTGGAGATGTTTACTGAGGATGGTGAAACCATGGATACTCCAAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCACCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTCTTTGATCATGGTGATCACACCCTTCAGAGGCGGGTTGTTGAGAGTTATGTACGCCGGTTATACCAGCCCTACCTTGTAAAGGGGAGTGTCAGGATGCAGTGGCACAGATCTGGTCTAATAGCTTCATGGGAGTTCTCAGAAGAACCTGTTGAGAGGAAACATGAGATTGAAGGTCAATCAGTTGATAAAACATTCCTTCAGAAACACGAGGAGAGGAAATGGGGAGTCATGGTTATAATAAAATCTCTTCAGTTTCTTCCAGCAATTATTAGTGGTGCACTGAAGGAAATGTCTCATAACCTTCATGAAGCAACTCCAAATGGATCTACTGAATCAAGTGGCTTTGGTAATATGATGCATATTGCTTTGGTTGGCATCAACAATCAAATGAGTCTACTTCAGGATAGTGGTGACGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCCAAAATACTTAAAGAGCAAGGAGTAGCCTCCAGTCTACACAGTGCAGGTGTCACAGTAATTAGTTGTATCATACAAAGGGATGAAGGGCGGGCCCCTATGAGGCACTCCTTCCACTGGTCATCAGAGAAGCTCTATTATGAAGAAGAACCCCTATTGCGTCACCTAGAACCTCCTCTATCTATTTACCTTGAATTGGACAAGCTTAAAGGCTATGAGAATATACAGTATACCCCATCTCGTGACCGTCAATGGCACTTATACACCGTTGTAGACAAGACAGTGAAGCCACCACCAATCCAAAGGATGTTCCTCAGAACACTTGTCAGGCAGCCCACAACAAATGAAGGGTTCTCTGGATTTCAACAGCTAGACATTGCAACAGCTAGTAACCAATTTTCTTTATCATTTACTTCAAGGAGCATCTTGAGGTCCTTATCAACAGCAATGGAAGAGTTGGAACTGAATGCGCACAATGCCACTGTCAAATCTGATCACACCCATATGTATCTTTATATCTTACGTGAGCAACAAATTAATGATCTGCTGCCTTACCACAAGAGAGTTGATTTAGATGCTCAACAAGAAGAAACTGTTGTGGAGGCCATCTTGGACGAACTTGCACGTGAAATCCACACATCAGTTGGTGTAAGGATGCATAGGTTGGGTGTTTGTGAGTGGGAAGTGAAGCTGTGGATGGCATCCTCTGGAGAGGAAAACGTTGCTTGGAGGGTTGTCGTGACAAATGTGACTGGTCATACCTGCACTGTACATATATATCGGGAACTAGAAGATACCAGCAAACAGGGAGCGGTATACCATTCAGTCTCTGTACAGGGTCCCCTGCATGGTGTACCGGTGAATGCGCACTATCAGCCTTTGGGAATTATAGACCGAAAACGTCTGTTGGCCAGGAGAACCAACACCACTTACTGCTATGATTTTCCACTGGCATTTGAGACGGCCTTGGAACAGTCATGGGCATCCCAGTCACCAGGTGTTGACAAACCGAAGGGTAAAATTCTCAAGGTCACAGAGCTCAAATTCGCTGACCAAAAAGGCAGCTGGGGTACTCCTCTCAATACCGTAGAGCGTCCACCTGGACTCAATGATGTTGGTATGATAGCCTGGTTTATGGAGATGTCTACCCCTGAGTTCCCTTCTGGAAGGAGGATATTGGTTGTTGCGAATGATGTCACCTACAAAGCTGGATCTTTTGGCCCAAGGGAGGATGCATTCTTCTTTGCAGTAACTGAGCTTGCTTGTGCTGAGAAATTGCCTCTAATATACTTGGCTGCAAACTCAGGTGCCCGTATTGGGGTTGCTGAAGAAGTCAAATCCTGTTTTAAAGTGGGCTGGTCTGATGAATCAAGCCCCGAACGTGGTTTTCAATATGTGTATTTAACCTCTGAGGATTATGCTCGGATTGGATCATCTGTGATAGCGCATGAATTAAACCTAGCAAATGGCGAAACCAGATGGGTGATAGATACCATTGTTGGAAAGGAGGACGGCTTGGGGGTCGAGAGCTTGACTGGAAGTGGGGCAATTGCTGGTGCATATTCGAGGGCATATATGGAAACCTTTACCTTAACATATGTGACTGGCAGAACTGTAGGGATAGGTGCTTATCTTGCAAGGCTTGGGATGCGGTGCATCCAGAGGCTTGATCAGCCCATCATTTTAACTGGTTTCTCTGCATTAAACAAACTACTTGGCCGGGAGGTTTACAGCTCCCAGATGCAACTTGGAGGACCTAAAATTATGGGAACTAATGGGGTTGTACATCTAACGGTAGCTGATGATCTTGAAGGAATATCTGCTATTCTAAAGTGGCTGAGCTATGTTCCTCCTCATATTGGTGGCCCACTCCCCATTTCAATTCCCTTGGATCCTCCAGAAAGGCGTGTGGAGTACTGCCCCGAGAATTCATGTGATCCTCGCGCTGCGATTTCTGGTGCTCTGAATGGTAATGGGAACTGGATGGGGGGTATATTTGACAAAGACAGCTTTGTTGAGACACTAGAAGGCTGGGCAAGGACGGTTGTTACTGGAAGGGCGAAGCTTGGAGGAATCCCTGTGGGAATTGTTGCTGTTGAGACACAGACGGTGATGCAAATTATTCCTGCTGATCCAGGCCAGCTTGATTCCCATGAGAGGGTTGTTCCTCAGGCTGGACAAGTATGGTTTCCTGATTCTGCAACTAAGACTGCCCAAGCATTATTAGATTTCAACAGAGAAGGACTCCCACTTTTCATTCTTGCCAACTGGAGAGGCTTTTCTGGTGGGCAGAGAGACCTTTTCGAAGGGATCCTTCAGGCTGGATCAACCATTGTGGAGAACCTAAGGACTTACAAACAGCCCGTTTTTGTATTCATCCCAATGATGGGTGAGCTCCGCGGTGGAGCATGGGTGGTCGTTGATAGCCGGATAAATCCGGACCATATTGAAATGTATGCTGATCGAACGGCTAGAGGAAATGTGCTTGAGCCAGAGGGGATGATCGAGATCAAGTTTAGGAACAAGGAATTGCTGGAGTGCATGGGCAGGCTTGACCAACAGCTGATCCAACTAAAGACTAAGCTTCAGGAAGCCAGGAGCTGTGGGGCGAATGAGAAGGTTGAATCCCTGCAGTTTCAGATACGATCCCGAGAAAAACAGCTTTTGCCCGTCTACACTCAGATAGCTACCAAATTTGCAGAACTTCATGATACGTCTCTGAGGATGGCTGCAAAAGGGGTAATCAGAGGAGTTTTGGAGTGGGCCAGCTCTCGATCTTTCTTCTACAAGAGATTGCGTCGGAGAATTGCCGATGACTCGCTCATCAAGATAGTGAGAGATGCTGCTGGTGAACAGCTGTCACATAGATCTGCAATGGATTTGATCAAGAGCTGGTTTTTGAGTTCTGATATTTCAAGAGGCAGGGAAAATGCTTGGGAGGATGATGAAACCTTCTTTAGATGGAAGGATGATCAAACTAATTATGAGGGTAAGCTAAAAGAATTGCGGGTCCAGAAGGTATTGCTTCAATTGGCAACCATTGGCAACTCAGTATCTGATTTGCAAGCCCTACCTCAAGGTCTTGCTGCCCTTCTAAGCAAGGTTGAGCCATCAAGCAGATCATTTTTGGTTGAGGAACTCCGGAAGGTGCTTGGTTGA

Protein Analysis

2271

Amino Acids

253.06

Weight (kDa)

5.96

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 49 - 168 5.1e-28 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 216 - 398 5.1e-46 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 217 - 368 2.7e-07 ATP-grasp domain
ATP-grasp_3 PF02655 223 - 370 2.3e-07 ATP-grasp domain
Biotin_carb_C PF02785 445 - 551 1.1e-23 Biotin carboxylase C-terminal domain
ACCA_BT PF21385 567 - 682 3.9e-29 Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl PF00364 693 - 755 1e-08 Biotin-requiring enzyme
ACC_central PF08326 756 - 1502 2.5e-179 Acetyl-CoA carboxylase, central region
Carboxyl_trans PF01039 1604 - 2155 4.5e-149 Carboxyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3446
AarI CACCTGC 4 cut(s) 109, 1099, 2200, 3695
AatII GACGTC 2 cut(s) 1265, 2708
Acc16I TGCGCA 2 cut(s) 4128, 4524
Acc36I ACCTGC 5 cut(s) 109, 1099, 2200, 3695, 4427
AccB1I GGYRCC 2 cut(s) 927, 4964
AccB7I CCANNNNNTGG 2 cut(s) 575, 1361
AccBSI CCGCTC 1 cut(s) 4469
AccI GTMKAC 7 cut(s) 3604, 3694, 3887, 3930, 4473, 4802, 6330
AccII CGCG 3 cut(s) 1941, 5601, 6057
AccIII TCCGGA 2 cut(s) 6094, 6798
AclI AACGTT 1 cut(s) 4377
AcoI YGGCCR 4 cut(s) 255, 261, 4566, 5374
AcvI CACGTG 1 cut(s) 4283
AcyI GRCGYC 2 cut(s) 1262, 2705
AdeI CACNNNGTG 2 cut(s) 1131, 4655
AflIII ACRYGT 3 cut(s) 394, 583, 1486
AgeI ACCGGT 1 cut(s) 4513
AhlI ACTAGT 1 cut(s) 1376
AjiI CACGTC 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 2329, 2361
AleI CACNNNNGTG 1 cut(s) 903
AloI GAACNNNNNNTCC 2 cut(s) 2460, 2492
Alw21I GWGCWC 8 cut(s) 1119, 1516, 2874, 3178, 3490, 4698, 5620, 6054
Alw44I GTGCAC 2 cut(s) 3174, 3486
AlwNI CAGNNNCTG 8 cut(s) 101, 1043, 1127, 1396, 4487, 4910, 6258, 6713
Ama87I CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
Aor13HI TCCGGA 2 cut(s) 6094, 6798
ApaI GGGCCC 1 cut(s) 3754
ApaLI GTGCAC 2 cut(s) 3174, 3486
AseI ATTAAT 2 cut(s) 536, 4194
AsiGI ACCGGT 1 cut(s) 4513
Asp700I GAANNNNTTC 1 cut(s) 3079
AspLEI GCGC 5 cut(s) 2823, 4129, 4525, 5104, 5603
AsuC2I CCSGG 1 cut(s) 5378
AsuII TTCGAA 1 cut(s) 5961
AvaI CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
AvaII GGWCC 9 cut(s) 84, 748, 950, 1877, 4089, 4496, 5411, 6097, 6667
AxyI CCTNAGG 2 cut(s) 5826, 6002
BaeGI GKGCMC 4 cut(s) 3178, 3490, 3754, 4969
BaeI ACNNNNGTAYC 4 cut(s) 2918, 2951, 5139, 5172
BalI TGGCCA 3 cut(s) 257, 263, 4568
BamHI GGATCC 3 cut(s) 1351, 5548, 5967
BanI GGYRCC 2 cut(s) 927, 4964
BanII GRGCYC 5 cut(s) 1516, 2668, 3754, 4698, 6054
BarI GAAGNNNNNNTAC 6 cut(s) 549, 581, 846, 878, 1911, 1943
BauI CACGAG 2 cut(s) 3416, 3897
BbrPI CACGTG 1 cut(s) 4283
BbsI GAAGAC 3 cut(s) 32, 279, 2009
Bbv12I GWGCWC 8 cut(s) 1119, 1516, 2874, 3178, 3490, 4698, 5620, 6054
BceAI ACGGC 4 cut(s) 888, 4635, 5185, 6141
BcgI CGANNNNNNTGC 2 cut(s) 983, 1017
BciVI GTATCC 1 cut(s) 3115
BclI TGATCA 8 cut(s) 310, 871, 3214, 3223, 4150, 5326, 6540, 6628
BcnI CCSGG 1 cut(s) 5378
BcuI ACTAGT 1 cut(s) 1376
BfmI CTRYAG 6 cut(s) 93, 102, 960, 1888, 5271, 6287
BfoI RGCGCY 1 cut(s) 2824
BfuAI ACCTGC 5 cut(s) 109, 1099, 2200, 3695, 4427
BfuI GTATCC 1 cut(s) 3115
BglII AGATCT 4 cut(s) 1597, 2845, 3317, 6524
BlpI GCTNAGC 1 cut(s) 5495
BmcAI AGTACT 3 cut(s) 1079, 1466, 5573
Bme18I GGWCC 9 cut(s) 84, 748, 950, 1877, 4089, 4496, 5411, 6097, 6667
BmeT110I CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
BmgBI CACGTC 1 cut(s) 210
BmrI ACTGGG 3 cut(s) 1115, 1471, 4639
BmuI ACTGGG 3 cut(s) 1115, 1471, 4639
BpiI GAAGAC 3 cut(s) 32, 279, 2009
BplI GAGNNNNNCTC 2 cut(s) 5886, 5918
BpmI CTGGAG 7 cut(s) 1467, 2220, 3673, 4386, 5538, 5947, 6212
Bpu10I CCTNAGC 2 cut(s) 441, 1797
Bpu1102I GCTNAGC 1 cut(s) 5495
Bpu14I TTCGAA 1 cut(s) 5961
BpuEI CTTGAG 8 cut(s) 528, 1796, 2645, 3621, 4105, 4667, 6164, 6717
BpuMI CCSGG 1 cut(s) 5378
Bsa29I ATCGAT 1 cut(s) 2130
BsaAI YACGTR 4 cut(s) 61, 197, 4181, 4283
BsaBI GATNNNNATC 2 cut(s) 2058, 6164
BsaHI GRCGYC 2 cut(s) 1262, 2705
BsaI GGTCTC 2 cut(s) 3212, 5946
BsaWI WCCGGW 3 cut(s) 4513, 6094, 6798
BsaXI ACNNNNNCTCC 6 cut(s) 949, 979, 3426, 3456, 6054, 6084
Bse118I RCCGGY 2 cut(s) 3264, 4513
Bse21I CCTNAGG 2 cut(s) 5826, 6002
Bse3DI GCAATG 5 cut(s) 2241, 3066, 4032, 4110, 6537
Bse8I GATNNNNATC 2 cut(s) 2058, 6164
BseAI TCCGGA 2 cut(s) 6094, 6798
BseCI ATCGAT 1 cut(s) 2130
BseJI GATNNNNATC 2 cut(s) 2058, 6164
BseMI GCAATG 5 cut(s) 2241, 3066, 4032, 4110, 6537
BseRI GAGGAG 8 cut(s) 125, 959, 1578, 3434, 3822, 4722, 5499, 6420
BseSI GKGCMC 4 cut(s) 3178, 3490, 3754, 4969
BseYI CCCAGC 3 cut(s) 697, 4722, 5688
BsgI GTGCAG 2 cut(s) 3723, 4405
Bsh1236I CGCG 3 cut(s) 1941, 5601, 6057
BshNI GGYRCC 2 cut(s) 927, 4964
BshTI ACCGGT 1 cut(s) 4513
BshVI ATCGAT 1 cut(s) 2130
BsiHKAI GWGCWC 8 cut(s) 1119, 1516, 2874, 3178, 3490, 4698, 5620, 6054
BsiHKCI CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
BsiSI CCGG 6 cut(s) 3265, 4514, 5377, 6085, 6095, 6799
BslFI GGGAC 4 cut(s) 70, 84, 1729, 4482
BsmBI CGTCTC 2 cut(s) 4610, 6378
BsmFI GGGAC 4 cut(s) 70, 84, 1729, 4482
BsmI GAATGC 5 cut(s) 1182, 2498, 4129, 4525, 4892
Bso31I GGTCTC 2 cut(s) 3212, 5946
BsoBI CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
Bsp119I TTCGAA 1 cut(s) 5961
Bsp120I GGGCCC 1 cut(s) 3750
Bsp13I TCCGGA 2 cut(s) 6094, 6798
Bsp1407I TGTACA 4 cut(s) 1056, 4426, 4489, 5440
Bsp1720I GCTNAGC 1 cut(s) 5495
Bsp19I CCATGG 4 cut(s) 258, 569, 2308, 3117
BspDI ATCGAT 1 cut(s) 2130
BspEI TCCGGA 2 cut(s) 6094, 6798
BspFNI CGCG 3 cut(s) 1941, 5601, 6057
BspHI TCATGA 3 cut(s) 1459, 3514, 6364
BspMAI CTGCAG 3 cut(s) 97, 106, 6291
BspMI ACCTGC 5 cut(s) 109, 1099, 2200, 3695, 4427
BspT104I TTCGAA 1 cut(s) 5961
BspT107I GGYRCC 2 cut(s) 927, 4964
BspTNI GGTCTC 2 cut(s) 3212, 5946
BsrBI CCGCTC 1 cut(s) 4469
BsrDI GCAATG 5 cut(s) 2241, 3066, 4032, 4110, 6537
BsrFI RCCGGY 2 cut(s) 3264, 4513
BsrGI TGTACA 4 cut(s) 1056, 4426, 4489, 5440
BssAI RCCGGY 2 cut(s) 3264, 4513
BssNAI GTATAC 2 cut(s) 3888, 4474
BssNI GRCGYC 2 cut(s) 1262, 2705
BssSI CACGAG 2 cut(s) 3416, 3897
BssT1I CCWWGG 9 cut(s) 127, 258, 569, 1691, 2308, 3117, 4623, 4880, 5544
Bst1107I GTATAC 2 cut(s) 3888, 4474
Bst2BI CACGAG 2 cut(s) 3416, 3897
Bst6I CTCTTC 4 cut(s) 2716, 2757, 3462, 4104
BstACI GRCGYC 2 cut(s) 1262, 2705
BstAPI GCANNNNNTGC 3 cut(s) 101, 461, 2443
BstAUI TGTACA 4 cut(s) 1056, 4426, 4489, 5440
BstBAI YACGTR 4 cut(s) 61, 197, 4181, 4283
BstBI TTCGAA 1 cut(s) 5961
BstDSI CCRYGG 5 cut(s) 258, 569, 2308, 3117, 6055
BstEII GGTNACC 1 cut(s) 692
BstENI CCTNNNNNAGG 1 cut(s) 3287
BstFNI CGCG 3 cut(s) 1941, 5601, 6057
BstH2I RGCGCY 1 cut(s) 2824
BstHHI GCGC 5 cut(s) 2823, 4129, 4525, 5104, 5603
BstNSI RCATGY 5 cut(s) 398, 587, 648, 1490, 1610
BstPI GGTNACC 1 cut(s) 692
BstSFI CTRYAG 6 cut(s) 93, 102, 960, 1888, 5271, 6287
BstSLI GKGCMC 4 cut(s) 3178, 3490, 3754, 4969
BstSNI TACGTA 2 cut(s) 61, 197
BstUI CGCG 3 cut(s) 1941, 5601, 6057
BstV2I GAAGAC 3 cut(s) 32, 279, 2009
BstXI CCANNNNNNTGG 1 cut(s) 1422
BstZ17I GTATAC 2 cut(s) 3888, 4474
Bsu15I ATCGAT 1 cut(s) 2130
Bsu36I CCTNAGG 2 cut(s) 5826, 6002
BsuI GTATCC 1 cut(s) 3115
BsuTUI ATCGAT 1 cut(s) 2130
BtgI CCRYGG 5 cut(s) 258, 569, 2308, 3117, 6055
BtgZI GCGATG 2 cut(s) 1763, 1956
BtrI CACGTC 1 cut(s) 210
BtsI GCAGTG 2 cut(s) 919, 3314
BveI ACCTGC 5 cut(s) 109, 1099, 2200, 3695, 4427
CaiI CAGNNNCTG 8 cut(s) 101, 1043, 1127, 1396, 4487, 4910, 6258, 6713
CciI TCATGA 3 cut(s) 1459, 3514, 6364
CfoI GCGC 5 cut(s) 2823, 4129, 4525, 5104, 5603
Cfr10I RCCGGY 2 cut(s) 3264, 4513
Cfr42I CCGCGG 1 cut(s) 6058
ClaI ATCGAT 1 cut(s) 2130
CseI GACGC 3 cut(s) 3806, 4739, 6443
CsiI ACCWGGT 1 cut(s) 4651
CspAI ACCGGT 1 cut(s) 4513
CspCI CAANNNNNGTGG 6 cut(s) 1264, 1299, 1625, 1660, 5973, 6008
DraI TTTAAA 4 cut(s) 1369, 1405, 1726, 5005
DraIII CACNNNGTG 2 cut(s) 1131, 4655
EaeI YGGCCR 4 cut(s) 255, 261, 4566, 5374
Eam1104I CTCTTC 4 cut(s) 2716, 2757, 3462, 4104
EarI CTCTTC 4 cut(s) 2716, 2757, 3462, 4104
Ecl136II GAGCTC 3 cut(s) 1514, 4696, 6052
Eco105I TACGTA 2 cut(s) 61, 197
Eco130I CCWWGG 9 cut(s) 127, 258, 569, 1691, 2308, 3117, 4623, 4880, 5544
Eco147I AGGCCT 1 cut(s) 1687
Eco24I GRGCYC 5 cut(s) 1516, 2668, 3754, 4698, 6054
Eco31I GGTCTC 2 cut(s) 3212, 5946
Eco47I GGWCC 9 cut(s) 84, 748, 950, 1877, 4089, 4496, 5411, 6097, 6667
Eco53kI GAGCTC 3 cut(s) 1514, 4696, 6052
Eco72I CACGTG 1 cut(s) 4283
Eco81I CCTNAGG 2 cut(s) 5826, 6002
Eco88I CYCGRG 5 cut(s) 482, 1509, 2736, 5579, 6306
Eco91I GGTNACC 1 cut(s) 692
EcoICRI GAGCTC 3 cut(s) 1514, 4696, 6052
EcoNI CCTNNNNNAGG 1 cut(s) 3287
EcoO109I RGGNCCY 7 cut(s) 84, 1877, 2330, 3751, 4089, 4496, 5411
EcoO65I GGTNACC 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 5584
EcoT14I CCWWGG 9 cut(s) 127, 258, 569, 1691, 2308, 3117, 4623, 4880, 5544
EcoT22I ATGCAT 5 cut(s) 430, 457, 3570, 4317, 4894
EcoT38I GRGCYC 5 cut(s) 1516, 2668, 3754, 4698, 6054
ErhI CCWWGG 9 cut(s) 127, 258, 569, 1691, 2308, 3117, 4623, 4880, 5544
Esp3I CGTCTC 2 cut(s) 4610, 6378
FalI AAGNNNNNCTT 8 cut(s) 1675, 1707, 2661, 2693, 3183, 3215, 5955, 5987
FaqI GGGAC 4 cut(s) 70, 84, 1729, 4482
FauI CCCGC 4 cut(s) 2522, 3234, 3741, 6657
FauNDI CATATG 3 cut(s) 2146, 4161, 5254
FbaI TGATCA 8 cut(s) 310, 871, 3214, 3223, 4150, 5326, 6540, 6628
FblI GTMKAC 7 cut(s) 3604, 3694, 3887, 3930, 4473, 4802, 6330
FriOI GRGCYC 5 cut(s) 1516, 2668, 3754, 4698, 6054
FspAI RTGCGCAY 2 cut(s) 4128, 4524
FspI TGCGCA 2 cut(s) 4128, 4524
GlaI GCGC 5 cut(s) 2822, 4128, 4524, 5103, 5602
GsaI CCCAGC 3 cut(s) 701, 4726, 5692
GsuI CTGGAG 7 cut(s) 1467, 2220, 3673, 4386, 5538, 5947, 6212
HaeII RGCGCY 1 cut(s) 2824
HapII CCGG 6 cut(s) 3265, 4514, 5377, 6085, 6095, 6799
HgaI GACGC 3 cut(s) 3806, 4739, 6443
HhaI GCGC 5 cut(s) 2823, 4129, 4525, 5104, 5603
Hin1I GRCGYC 2 cut(s) 1262, 2705
Hin6I GCGC 5 cut(s) 2821, 4127, 4523, 5102, 5601
HinP1I GCGC 5 cut(s) 2821, 4127, 4523, 5102, 5601
HincII GTYRAC 3 cut(s) 27, 1024, 4660
HindII GTYRAC 3 cut(s) 27, 1024, 4660
HindIII AAGCTT 5 cut(s) 2314, 2377, 3860, 5720, 6239
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 6 cut(s) 3265, 4514, 5377, 6085, 6095, 6799
Hpy99I CGWCG 1 cut(s) 6459
Hsp92I GRCGYC 2 cut(s) 1262, 2705
HspAI GCGC 5 cut(s) 2821, 4127, 4523, 5102, 5601
KflI GGGWCCC 1 cut(s) 4496
Kpn2I TCCGGA 2 cut(s) 6094, 6798
Ksp22I TGATCA 8 cut(s) 310, 871, 3214, 3223, 4150, 5326, 6540, 6628
KspAI GTTAAC 1 cut(s) 1024
KspI CCGCGG 1 cut(s) 6058
MabI ACCWGGT 1 cut(s) 4651
MbiI CCGCTC 1 cut(s) 4469
MfeI CAATTG 4 cut(s) 2216, 2449, 5205, 6686
MlsI TGGCCA 3 cut(s) 257, 263, 4568
MluNI TGGCCA 3 cut(s) 257, 263, 4568
MlyI GAGTC 9 cut(s) 1514, 1711, 1916, 2910, 3444, 3610, 4755, 5895, 6467
Mox20I TGGCCA 3 cut(s) 257, 263, 4568
Mph1103I ATGCAT 5 cut(s) 430, 457, 3570, 4317, 4894
MroI TCCGGA 2 cut(s) 6094, 6798
MroXI GAANNNNTTC 1 cut(s) 3079
MscI TGGCCA 3 cut(s) 257, 263, 4568
Msp20I TGGCCA 3 cut(s) 257, 263, 4568
MspA1I CMGCKG 9 cut(s) 494, 606, 1268, 2006, 2285, 4722, 6057, 6220, 6514
MspI CCGG 6 cut(s) 3265, 4514, 5377, 6085, 6095, 6799
MunI CAATTG 4 cut(s) 2216, 2449, 5205, 6686
Mva1269I GAATGC 5 cut(s) 1182, 2498, 4129, 4525, 4892
MvnI CGCG 3 cut(s) 1941, 5601, 6057
NciI CCSGG 1 cut(s) 5378
NcoI CCATGG 4 cut(s) 258, 569, 2308, 3117
NdeI CATATG 3 cut(s) 2146, 4161, 5254
NsbI TGCGCA 2 cut(s) 4128, 4524
NsiI ATGCAT 5 cut(s) 430, 457, 3570, 4317, 4894
NspI RCATGY 5 cut(s) 398, 587, 648, 1490, 1610
NspV TTCGAA 1 cut(s) 5961
OliI CACNNNNGTG 1 cut(s) 903
PaeR7I CTCGAG 1 cut(s) 1509
PagI TCATGA 3 cut(s) 1459, 3514, 6364
PaqCI CACCTGC 4 cut(s) 109, 1099, 2200, 3695
PceI AGGCCT 1 cut(s) 1687
PciI ACATGT 3 cut(s) 394, 583, 1486
PctI GAATGC 5 cut(s) 1182, 2498, 4129, 4525, 4892
PdmI GAANNNNTTC 1 cut(s) 3079
PflMI CCANNNNNTGG 2 cut(s) 575, 1361
PfoI TCCNGGA 1 cut(s) 802
PinAI ACCGGT 1 cut(s) 4513
PleI GAGTC 9 cut(s) 1513, 1711, 1915, 2910, 3443, 3609, 4755, 5895, 6467
PmaCI CACGTG 1 cut(s) 4283
PmlI CACGTG 1 cut(s) 4283
PpsI GAGTC 9 cut(s) 1513, 1711, 1915, 2910, 3443, 3609, 4755, 5895, 6467
Ppu21I YACGTR 4 cut(s) 61, 197, 4181, 4283
PpuMI RGGWCCY 5 cut(s) 84, 1877, 4089, 4496, 5411
PscI ACATGT 3 cut(s) 394, 583, 1486
PshBI ATTAAT 2 cut(s) 536, 4194
PsiI TTATAA 1 cut(s) 3446
Psp124BI GAGCTC 3 cut(s) 1516, 4698, 6054
Psp1406I AACGTT 1 cut(s) 4377
Psp5II RGGWCCY 5 cut(s) 84, 1877, 4089, 4496, 5411
PspCI CACGTG 1 cut(s) 4283
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 3 cut(s) 697, 4722, 5688
PspOMI GGGCCC 1 cut(s) 3750
PspPPI RGGWCCY 5 cut(s) 84, 1877, 4089, 4496, 5411
PstI CTGCAG 3 cut(s) 97, 106, 6291
PstNI CAGNNNCTG 8 cut(s) 101, 1043, 1127, 1396, 4487, 4910, 6258, 6713
PvuII CAGCTG 8 cut(s) 494, 606, 1268, 2006, 2285, 4722, 6220, 6514
SacI GAGCTC 3 cut(s) 1516, 4698, 6054
SacII CCGCGG 1 cut(s) 6058
ScaI AGTACT 3 cut(s) 1079, 1466, 5573
SchI GAGTC 9 cut(s) 1514, 1711, 1916, 2910, 3444, 3610, 4755, 5895, 6467
SexAI ACCWGGT 1 cut(s) 4651
SfcI CTRYAG 6 cut(s) 93, 102, 960, 1888, 5271, 6287
Sfr274I CTCGAG 1 cut(s) 1509
Sfr303I CCGCGG 1 cut(s) 6058
SfuI TTCGAA 1 cut(s) 5961
SgrBI CCGCGG 1 cut(s) 6058
SinI GGWCC 9 cut(s) 84, 748, 950, 1877, 4089, 4496, 5411, 6097, 6667
SlaI CTCGAG 1 cut(s) 1509
SmlI CTYRAG 9 cut(s) 543, 1509, 1775, 2624, 3636, 4084, 4682, 6143, 6732
SmoI CTYRAG 9 cut(s) 543, 1509, 1775, 2624, 3636, 4084, 4682, 6143, 6732
SnaBI TACGTA 2 cut(s) 61, 197
SpeI ACTAGT 1 cut(s) 1376
SseBI AGGCCT 1 cut(s) 1687
SstI GAGCTC 3 cut(s) 1516, 4698, 6054
StuI AGGCCT 1 cut(s) 1687
StyI CCWWGG 9 cut(s) 127, 258, 569, 1691, 2308, 3117, 4623, 4880, 5544
TaqII GACCGA 1 cut(s) 4566
TatI WGTACW 7 cut(s) 1056, 1077, 1464, 4426, 4489, 5440, 5571
TauI GCSGC 2 cut(s) 1001, 1748
Van91I CCANNNNNTGG 2 cut(s) 575, 1361
VneI GTGCAC 2 cut(s) 3174, 3486
VpaK11BI GGWCC 9 cut(s) 84, 748, 950, 1877, 4089, 4496, 5411, 6097, 6667
VspI ATTAAT 2 cut(s) 536, 4194
XagI CCTNNNNNAGG 1 cut(s) 3287
XceI RCATGY 5 cut(s) 398, 587, 648, 1490, 1610
XcmI CCANNNNNNNNNTGG 4 cut(s) 1128, 1169, 4298, 5402
XhoI CTCGAG 1 cut(s) 1509
XmiI GTMKAC 7 cut(s) 3604, 3694, 3887, 3930, 4473, 4802, 6330
XmnI GAANNNNTTC 1 cut(s) 3079
ZraI GACGTC 2 cut(s) 1263, 2706
ZrmI AGTACT 3 cut(s) 1079, 1466, 5573
Zsp2I ATGCAT 5 cut(s) 430, 457, 3570, 4317, 4894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.