FvH4_6g08370

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002178
Physical Location & Seq
Forward (+)
4946457 .. 4949245
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08370.t2

Sequence Viewer

Length: 1161 bp
ATGGACACCTTCCTCTTTACCTCCGAGTCTGTGAATGAGGGACACCCTGATAAGCTCTGTGATCAGATTTCCGATGCAGTGCTTGATGCCTGCCTTGAACAAGATCCTGACAGCAAAGTTGCCTGTGAGACTTGCACCAAGACCAACATGGTCATGGTTTTCGGAGAGATCACAACCAAAGCCAAAGTGGACTATGAGAAGATAGTGCGCGACACCTGCCGCAGCATTGGATTTGTTTCGGATGCTGTAGGCCTTGATGCTGACAACTGCAAGGTCCTGGTGAACATTGAGCAACAAAGCCCTGATATAGCTCAGGGTGTGCATGGCCACCTCACCAAGCGGCCCGAGGAAATTGGTGCAGGAGACCAGGGTCACATGTTTGGTTATGCCACTGATGAGACTCCTGAGCTTATGCCTCTCAGCCATGTTCTTGCTACACAGCTTGGTGCACGTCTCACTGATGTTCGAAAAAATGGCACCTGCCCTTGGCTGAGACCTGATGGCAAGACTCAAGTCACTGTGGAGTACTACAATGACCATGGTGCTATGGTTCCACTCCGTGTCCACACGGTTCTCATTTCTACTCAGCATGATGAGACTGTTACCAATGATGAAATTGCAGCTGACCTCAAGAAACATGTCATCAAGCCAGTTGTTCCTGAGAAGTACCTAGATGAGAAGACTATTTTCCATCTCAACCCTTCAGGTCGATTTGTGATTGGTGGACCGCACGGTGATGCAGGTCTAACTGGCCGAAAGATCATCATCGACACCTATGGTGGTTGGGGAGCTCATGGTGGTGGTGCCTTCTCCGGCAAGGACCCTACCAAGGTAGACAGGAGCGGTGCATACATTGTTAGGCAGGCTGCTAAGAGCATTGTAGCCAATGGCCTTGCTAGAAGGTGCATAGTGCAGGTCTCATATGCAATTGGTGTACCTGAGCCACTATCAGTGTTTGTTGACACTTATAAGACTGGAAAGATTCCAGACAAGGAGATTCTGAAGTTAGTGAAGGAGGCCTTTGATTTTAGGCCTGGAATGATTGCCATTAACTTGGACCTCAAGAGGGGAAGGTATTTGAAGACAGCTGCTTATGGACACTTTGGTAGAGATGATCCTGACTTTACATGGGAAGTGGTGAAGACCTTGAAGTGGGAATAG

Protein Analysis

387

Amino Acids

42.43

Weight (kDa)

5.83

Isoelectric Point (pI)

24.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 2.9e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 5.5e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 240 - 378 1.7e-62 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 969
AarI CACCTGC 2 cut(s) 224, 488
Acc36I ACCTGC 4 cut(s) 224, 488, 731, 904
AccB1I GGYRCC 2 cut(s) 476, 803
AccBSI CCGCTC 1 cut(s) 843
AccI GTMKAC 1 cut(s) 834
AccII CGCG 1 cut(s) 210
AciI CCGC 4 cut(s) 220, 340, 728, 843
AclWI GGATC 2 cut(s) 98, 1109
AcoI YGGCCR 2 cut(s) 325, 751
AcuI CTGAAG 2 cut(s) 687, 1022
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 3 cut(s) 527, 668, 936
AfiI CCNNNNNNNGG 4 cut(s) 486, 829, 1066, 1152
AflIII ACRYGT 2 cut(s) 375, 637
AgsI TTSAA 3 cut(s) 98, 1081, 1150
AjiI CACGTC 1 cut(s) 452
AjnI CCWGG 3 cut(s) 276, 366, 1033
AluBI AGCT 7 cut(s) 55, 311, 409, 442, 623, 791, 1088
AluI AGCT 7 cut(s) 55, 311, 409, 442, 623, 791, 1088
Alw21I GWGCWC 2 cut(s) 451, 793
Alw26I GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 2 cut(s) 98, 1109
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 7 cut(s) 250, 325, 341, 751, 889, 1017, 1031
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 4 cut(s) 222, 620, 866, 1088
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 5 cut(s) 274, 342, 725, 820, 1057
AsuHPI GGTGA 4 cut(s) 292, 325, 746, 1150
AsuII TTCGAA 1 cut(s) 466
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 4 cut(s) 274, 725, 820, 1057
BaeGI GKGCMC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 2 cut(s) 476, 803
BanII GRGCYC 1 cut(s) 793
BbsI GAAGAC 3 cut(s) 686, 1088, 1148
Bbv12I GWGCWC 2 cut(s) 451, 793
BbvI GCAGC 4 cut(s) 234, 632, 853, 1075
BccI CCATC 2 cut(s) 494, 699
BciT130I CCWGG 3 cut(s) 278, 368, 1035
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BfaI CTAG 2 cut(s) 671, 897
BfmI CTRYAG 1 cut(s) 246
BfuAI ACCTGC 4 cut(s) 224, 488, 731, 904
BisI GCNGC 6 cut(s) 220, 223, 341, 621, 867, 1089
BlsI GCNGC 6 cut(s) 221, 224, 342, 622, 868, 1090
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 3 cut(s) 278, 368, 1035
Bme18I GGWCC 4 cut(s) 274, 725, 820, 1057
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 5 cut(s) 274, 342, 725, 820, 1057
BmiI GGNNCC 4 cut(s) 478, 552, 805, 822
BmrFI CCNGG 3 cut(s) 278, 368, 1035
BmsI GCATC 5 cut(s) 64, 76, 232, 247, 727
BoxI GACNNNNGTC 2 cut(s) 369, 512
BpiI GAAGAC 3 cut(s) 686, 1088, 1148
Bpu10I CCTNAGC 3 cut(s) 312, 405, 939
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 3 cut(s) 495, 614, 1046
BsaBI GATNNNNATC 1 cut(s) 764
BsaI GGTCTC 3 cut(s) 357, 487, 922
BsaJI CCNNGG 5 cut(s) 345, 367, 485, 538, 828
Bsc4I CCNNNNNNNGG 4 cut(s) 486, 829, 1066, 1152
Bse1I ACTGG 3 cut(s) 650, 754, 979
Bse8I GATNNNNATC 1 cut(s) 764
BseBI CCWGG 3 cut(s) 278, 368, 1035
BseDI CCNNGG 5 cut(s) 345, 367, 485, 538, 828
BseGI GGATG 1 cut(s) 247
BseJI GATNNNNATC 1 cut(s) 764
BseLI CCNNNNNNNGG 4 cut(s) 486, 829, 1066, 1152
BseMII CTCAG 7 cut(s) 326, 396, 433, 482, 599, 651, 930
BseNI ACTGG 3 cut(s) 650, 754, 979
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 4 cut(s) 234, 632, 853, 1075
BsgI GTGCAG 2 cut(s) 378, 932
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 7 cut(s) 252, 327, 343, 753, 891, 1019, 1033
BshNI GGYRCC 2 cut(s) 476, 803
BsiHKAI GWGCWC 2 cut(s) 451, 793
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 1 cut(s) 813
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 4 cut(s) 486, 829, 1066, 1152
BsmAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 7 cut(s) 252, 327, 343, 753, 891, 1019, 1033
Bso31I GGTCTC 3 cut(s) 357, 487, 922
BsoBI CYCGRG 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 451, 793
Bsp143I GATC 5 cut(s) 61, 103, 168, 759, 1114
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 4 cut(s) 220, 340, 728, 843
BspANI GGCC 7 cut(s) 252, 327, 343, 753, 891, 1019, 1033
BspCNI CTCAG 7 cut(s) 325, 397, 432, 483, 598, 652, 931
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 4 cut(s) 478, 552, 805, 822
BspMI ACCTGC 4 cut(s) 224, 488, 731, 904
BspPI GGATC 2 cut(s) 98, 1109
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 2 cut(s) 476, 803
BspTNI GGTCTC 3 cut(s) 357, 487, 922
BsrBI CCGCTC 1 cut(s) 843
BsrI ACTGG 3 cut(s) 650, 754, 979
BssECI CCNNGG 5 cut(s) 345, 367, 485, 538, 828
BssMI GATC 5 cut(s) 61, 103, 168, 759, 1114
BssT1I CCWWGG 3 cut(s) 485, 538, 828
Bst2UI CCWGG 3 cut(s) 278, 368, 1035
Bst4CI ACNGT 4 cut(s) 520, 571, 601, 734
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 91, 864
BstDEI CTNAG 8 cut(s) 312, 405, 419, 491, 585, 660, 870, 939
BstDSI CCRYGG 1 cut(s) 538
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 5 cut(s) 64, 106, 171, 762, 1117
BstMAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BstMBI GATC 5 cut(s) 61, 103, 168, 759, 1114
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 3 cut(s) 278, 368, 1035
BstNSI RCATGY 2 cut(s) 379, 641
BstPAI GACNNNNGTC 2 cut(s) 369, 512
BstSCI CCNGG 3 cut(s) 276, 366, 1033
BstSFI CTRYAG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 451
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 234, 632, 853, 1075
BstV2I GAAGAC 3 cut(s) 686, 1088, 1148
BstX2I RGATCY 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 7 cut(s) 252, 327, 343, 753, 891, 1019, 1033
BtgI CCRYGG 1 cut(s) 538
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 5 cut(s) 84, 390, 456, 516, 957
BveI ACCTGC 4 cut(s) 224, 488, 731, 904
Cac8I GCNNGC 2 cut(s) 91, 864
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 5 cut(s) 274, 342, 725, 820, 1057
Csp6I GTAC 3 cut(s) 526, 667, 935
CviQI GTAC 3 cut(s) 526, 667, 935
DdeI CTNAG 8 cut(s) 312, 405, 419, 491, 585, 660, 870, 939
DpnI GATC 5 cut(s) 63, 105, 170, 761, 1116
DpnII GATC 5 cut(s) 61, 103, 168, 759, 1114
DraIII CACNNNGTG 1 cut(s) 560
EaeI YGGCCR 2 cut(s) 325, 751
Ecl136II GAGCTC 1 cut(s) 791
Eco130I CCWWGG 3 cut(s) 485, 538, 828
Eco147I AGGCCT 3 cut(s) 252, 1019, 1033
Eco24I GRGCYC 1 cut(s) 793
Eco31I GGTCTC 3 cut(s) 357, 487, 922
Eco47I GGWCC 4 cut(s) 274, 725, 820, 1057
Eco53kI GAGCTC 1 cut(s) 791
Eco57I CTGAAG 2 cut(s) 687, 1022
Eco88I CYCGRG 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 791
EcoO109I RGGNCCY 2 cut(s) 274, 820
EcoRII CCWGG 3 cut(s) 276, 366, 1033
EcoT14I CCWWGG 3 cut(s) 485, 538, 828
EcoT38I GRGCYC 1 cut(s) 793
ErhI CCWWGG 3 cut(s) 485, 538, 828
Esp3I CGTCTC 1 cut(s) 458
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 6 cut(s) 220, 223, 341, 621, 867, 1089
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 6 cut(s) 220, 223, 341, 621, 867, 1089
FspBI CTAG 2 cut(s) 671, 897
GlaI GCGC 1 cut(s) 209
GluI GCNGC 6 cut(s) 220, 223, 341, 621, 867, 1089
HaeIII GGCC 7 cut(s) 252, 327, 343, 753, 891, 1019, 1033
HapII CCGG 1 cut(s) 813
HhaI GCGC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HinfI GANTC 5 cut(s) 26, 400, 508, 982, 997
HpaII CCGG 1 cut(s) 813
HphI GGTGA 4 cut(s) 292, 325, 746, 1150
Hpy166II GTNNAC 8 cut(s) 190, 283, 449, 565, 725, 835, 935, 961
Hpy188I TCNGA 6 cut(s) 25, 66, 73, 164, 241, 1002
Hpy188III TCNNGA 7 cut(s) 107, 404, 631, 659, 986, 1063, 1118
Hpy8I GTNNAC 8 cut(s) 190, 283, 449, 565, 725, 835, 935, 961
HpyAV CCTTC 6 cut(s) 19, 711, 817, 894, 1006, 1065
HpyCH4III ACNGT 4 cut(s) 520, 571, 601, 734
HpyCH4IV ACGT 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 8 cut(s) 312, 405, 419, 491, 585, 660, 870, 939
HpySE526I ACGT 1 cut(s) 451
HspAI GCGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 103, 168, 759, 1114
LmnI GCTCC 2 cut(s) 788, 840
Lsp1109I GCAGC 4 cut(s) 234, 632, 853, 1075
LweI GCATC 5 cut(s) 64, 76, 232, 247, 727
MaeI CTAG 2 cut(s) 671, 897
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 3 cut(s) 371, 514, 601
MalI GATC 5 cut(s) 63, 105, 170, 761, 1116
MbiI CCGCTC 1 cut(s) 843
MboI GATC 5 cut(s) 61, 103, 168, 759, 1114
MboII GAAGA 4 cut(s) 211, 691, 1093, 1153
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 451, 793
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 3 cut(s) 351, 615, 927
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 3 cut(s) 35, 394, 502
Mox20I TGGCCA 1 cut(s) 327
MscI TGGCCA 1 cut(s) 327
MseI TTAA 1 cut(s) 1050
MslI CAYNNNNRTG 3 cut(s) 152, 735, 798
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 2 cut(s) 623, 1088
MspI CCGG 1 cut(s) 813
MspR9I CCNGG 3 cut(s) 278, 368, 1035
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 3 cut(s) 278, 368, 1035
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 61, 103, 168, 759, 1114
NlaIV GGNNCC 4 cut(s) 478, 552, 805, 822
NmuCI GTSAC 2 cut(s) 371, 514
NspI RCATGY 2 cut(s) 379, 641
NspV TTCGAA 1 cut(s) 466
PaqCI CACCTGC 2 cut(s) 224, 488
PceI AGGCCT 3 cut(s) 252, 1019, 1033
PciI ACATGT 2 cut(s) 375, 637
PfeI GAWTC 2 cut(s) 982, 997
PkrI GCNGC 6 cut(s) 221, 224, 342, 622, 868, 1090
PleI GAGTC 3 cut(s) 34, 394, 502
PpsI GAGTC 3 cut(s) 34, 394, 502
PpuMI RGGWCCY 2 cut(s) 274, 820
PscI ACATGT 2 cut(s) 375, 637
PshAI GACNNNNGTC 2 cut(s) 369, 512
PsiI TTATAA 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 793
Psp5II RGGWCCY 2 cut(s) 274, 820
Psp6I CCWGG 3 cut(s) 276, 366, 1033
PspGI CCWGG 3 cut(s) 276, 366, 1033
PspN4I GGNNCC 4 cut(s) 478, 552, 805, 822
PspPI GGNCC 5 cut(s) 274, 342, 725, 820, 1057
PspPPI RGGWCCY 2 cut(s) 274, 820
PsuI RGATCY 1 cut(s) 103
PvuII CAGCTG 2 cut(s) 623, 1088
RsaI GTAC 3 cut(s) 527, 668, 936
RsaNI GTAC 3 cut(s) 526, 667, 935
RseI CAYNNNNRTG 3 cut(s) 152, 735, 798
SacI GAGCTC 1 cut(s) 793
SaqAI TTAA 1 cut(s) 1050
SatI GCNGC 6 cut(s) 220, 223, 341, 621, 867, 1089
Sau3AI GATC 5 cut(s) 61, 103, 168, 759, 1114
Sau96I GGNCC 5 cut(s) 274, 342, 725, 820, 1057
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 3 cut(s) 35, 394, 502
ScrFI CCNGG 3 cut(s) 278, 368, 1035
SduI GDGCHC 2 cut(s) 451, 793
SfaNI GCATC 5 cut(s) 64, 76, 232, 247, 727
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 4 cut(s) 274, 725, 820, 1057
SmiMI CAYNNNNRTG 3 cut(s) 152, 735, 798
SmlI CTYRAG 3 cut(s) 510, 629, 1061
SmoI CTYRAG 3 cut(s) 510, 629, 1061
Sse9I AATT 3 cut(s) 351, 615, 927
SseBI AGGCCT 3 cut(s) 252, 1019, 1033
SsiI CCGC 4 cut(s) 220, 340, 728, 843
SspMI CTAG 2 cut(s) 671, 897
SstI GAGCTC 1 cut(s) 793
StuI AGGCCT 3 cut(s) 252, 1019, 1033
StyD4I CCNGG 3 cut(s) 276, 366, 1033
StyI CCWWGG 3 cut(s) 485, 538, 828
TaaI ACNGT 4 cut(s) 520, 571, 601, 734
TaiI ACGT 1 cut(s) 454
TaqI TCGA 3 cut(s) 466, 709, 768
TasI AATT 3 cut(s) 351, 615, 927
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 222, 343
TfiI GAWTC 2 cut(s) 982, 997
Tru1I TTAA 1 cut(s) 1050
Tru9I TTAA 1 cut(s) 1050
TscAI CASTG 5 cut(s) 84, 397, 463, 523, 957
TseFI GTSAC 2 cut(s) 371, 514
TseI GCWGC 4 cut(s) 222, 620, 866, 1088
Tsp45I GTSAC 2 cut(s) 371, 514
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 5 cut(s) 84, 397, 463, 523, 957
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 4 cut(s) 274, 725, 820, 1057
XceI RCATGY 2 cut(s) 379, 641
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 151, 184
XmiI GTMKAC 1 cut(s) 834
XspI CTAG 2 cut(s) 671, 897
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.