RLG00000023092

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002178
Physical Location & Seq
Forward (+)
17997194 .. 18000228
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023092

Sequence Viewer

Length: 1398 bp
ATGCCTCGTGCATTCATGCTCCATTCTTGTTCCACTTCCCATTTAAATATTCCCCTTCCTTTTTTTGGTTCTGAGTCAAATGGAAATAAAAAGATTACAAATCTAACCGAAGCGGTTTGTCGTTTCCAACCAAAGAGGCAAAAAAGCGGGGTCTGGTTCGAAAACCACAGCCTCCGTCTTCTTCCCTCCAAGTTTCAGATCTACGCTCTACAGAAAATGGAGACTTTCCTATTTACATCTGAATCTGTGAACGAGGGACACCCCGACAAGCTGTGCGACCAGATCTCTGATGCAGTGCTTGATGCTTGCCTTGAGCAGGACCCTGACAGCAAGGTTGCCTGTGAGACATGCACCAAAACAAACATGGTAATGGTGTTCGGAGAGATCACCACCAAGGCCACTGTTGACTACGAGAAGATTGTCCGTGACACATGCCGCAGCATTGGGTTTGTTTCTGATGATGTCGGTCTTGATGCCGATAACTGCAAGGTCCTAGTCAACATTGAGCAGCAGAGCCCAGATATTGCTCAGGGTGTCCATGGTCACTTTACCAAGCGCCCAGAGGAGATTGGTGCTGGTGACCAGGGTCACATGTTTGGTTATGCCACTGATGAAACCCCTGAGTTGATGCCCCTTAGCCATGTCCTTGCAACCAAGCTTGGTGCTCGCCTCACTGAAGTTAGAAAGAATGGTACTTGCGCATGGCTGAGACCTGATGGCAAGACCCAGGTGACAGTTGAATACCAGAATGAAGCCGGTGCTATGGTTCCACTACGTGTCCACACTGTTCTTATCTCCACCCAGCATGACGAGACTGTCACAAACGATGAGATTGCTGCTGACCTCAAGGAGCATGTTATTAAGCCTGTCATCCCCGAGAAGTACCTGGATGAGAAGACAATCTTCCATCTTAACCCCTCTGGTCGCTTTGTGATTGGTGGCCCTCATGGTGATGCAGGTCTCACCGGACGCAAGATCATTATTGATACCTATGGTGGTTGGGGAGCCCATGGTGGTGGTGCTTTCTCTGGAAAGGACCCTACCAAGGTGGACAGGAGTGGTGCTTACATTGTCAGGCAGGCTGCCAAGAGCATTGTAGCAAATGGTCTTGCTCGCAGGGCCCTCGTTCAGGTCTCCTATGCCATTGGTGTCCCTGAGCCCTTGTCGGTCTTTGTCGAAACATACGGAACTGGAAAGATCCCTGACAAGGAGATCCTTAAAATTGTGAAGGAGAACTTTGACTTCAGGCCGGGTATGATGACCATCAACCTGGACCTCAAGAGGGGTGGCAATGGAAGATTCTTGAAGACAGCCGCCTATGGGCACTTCGGAAGGGATGACCCTGACTTCACCTGGGAGGTTGTGAAGCCCCTCAAGTGGGAGAAGCCTCAGTCTTGA

Protein Analysis

466

Amino Acids

51.35

Weight (kDa)

6.33

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 76 - 173 3.6e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 189 - 310 1.8e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 312 - 453 5e-60 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 815
Acc16I TGCGCA 1 cut(s) 700
Acc36I ACCTGC 1 cut(s) 947
AciI CCGC 4 cut(s) 113, 147, 436, 1314
AclWI GGATC 2 cut(s) 1192, 1207
AcuI CTGAAG 2 cut(s) 696, 1228
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 2 cut(s) 694, 884
AfiI CCNNNNNNNGG 9 cut(s) 65, 316, 1045, 1129, 1207, 1282, 1320, 1377, 1378
AflIII ACRYGT 2 cut(s) 591, 775
AgsI TTSAA 2 cut(s) 740, 1306
AjnI CCWGG 5 cut(s) 582, 726, 885, 1269, 1352
AluBI AGCT 2 cut(s) 271, 658
AluI AGCT 2 cut(s) 271, 658
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 6 cut(s) 215, 338, 703, 806, 965, 1138
AlwI GGATC 2 cut(s) 1192, 1207
Ama87I CYCGRG 1 cut(s) 875
AoxI GGCC 4 cut(s) 396, 940, 1119, 1247
ApaI GGGCCC 1 cut(s) 1123
ApeKI GCWGC 4 cut(s) 438, 508, 836, 1082
AspLEI GCGC 2 cut(s) 558, 701
AspS9I GGNCC 7 cut(s) 319, 490, 941, 1036, 1119, 1120, 1273
AsuC2I CCSGG 1 cut(s) 1251
AsuHPI GGTGA 6 cut(s) 379, 590, 742, 955, 962, 1342
AsuII TTCGAA 1 cut(s) 159
AvaI CYCGRG 1 cut(s) 875
AvaII GGWCC 4 cut(s) 319, 490, 1036, 1273
BaeGI GKGCMC 2 cut(s) 1123, 1326
BanII GRGCYC 4 cut(s) 518, 1009, 1123, 1161
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 3 cut(s) 170, 902, 1313
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 4 cut(s) 450, 520, 823, 1069
BccI CCATC 3 cut(s) 710, 915, 1271
BcgI CGANNNNNNTGC 4 cut(s) 815, 849, 1105, 1139
BciT130I CCWGG 5 cut(s) 584, 728, 887, 1271, 1354
BcnI CCSGG 1 cut(s) 1251
BcoDI GTCTC 6 cut(s) 215, 338, 703, 806, 965, 1138
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 209
BfoI RGCGCY 1 cut(s) 559
BfuAI ACCTGC 1 cut(s) 947
BglII AGATCT 2 cut(s) 198, 282
BisI GCNGC 6 cut(s) 436, 439, 509, 837, 1083, 1314
BlsI GCNGC 6 cut(s) 437, 440, 510, 838, 1084, 1315
Bme1390I CCNGG 6 cut(s) 584, 728, 887, 1251, 1271, 1354
Bme18I GGWCC 4 cut(s) 319, 490, 1036, 1273
BmeT110I CYCGRG 1 cut(s) 875
BmgT120I GGNCC 7 cut(s) 319, 490, 941, 1036, 1119, 1120, 1273
BmiI GGNNCC 5 cut(s) 321, 768, 1006, 1038, 1121
BmrFI CCNGG 6 cut(s) 584, 728, 887, 1251, 1271, 1354
BmsI GCATC 5 cut(s) 280, 292, 463, 618, 943
BoxI GACNNNNGTC 1 cut(s) 585
BpiI GAAGAC 3 cut(s) 170, 902, 1313
Bpu10I CCTNAGC 3 cut(s) 528, 635, 1155
Bpu14I TTCGAA 1 cut(s) 159
BpuEI CTTGAG 4 cut(s) 332, 830, 1262, 1358
BpuMI CCSGG 1 cut(s) 1251
BsaAI YACGTR 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 1262
BsaI GGTCTC 3 cut(s) 703, 965, 1138
BsaJI CCNNGG 7 cut(s) 393, 538, 583, 726, 1009, 1044, 1353
BsaWI WCCGGW 1 cut(s) 965
Bsc4I CCNNNNNNNGG 9 cut(s) 65, 316, 1045, 1129, 1207, 1282, 1320, 1377, 1378
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 1195
Bse3DI GCAATG 1 cut(s) 1297
Bse8I GATNNNNATC 1 cut(s) 1262
BseBI CCWGG 5 cut(s) 584, 728, 887, 1271, 1354
BseDI CCNNGG 7 cut(s) 393, 538, 583, 726, 1009, 1044, 1353
BseGI GGATG 3 cut(s) 870, 895, 1342
BseJI GATNNNNATC 1 cut(s) 1262
BseLI CCNNNNNNNGG 9 cut(s) 65, 316, 1045, 1129, 1207, 1282, 1320, 1377, 1378
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 5 cut(s) 63, 542, 612, 698, 1146
BseNI ACTGG 1 cut(s) 1195
BseRI GAGGAG 1 cut(s) 578
BseSI GKGCMC 2 cut(s) 1123, 1326
BseXI GCAGC 4 cut(s) 450, 520, 823, 1069
BseYI CCCAGC 1 cut(s) 801
BshFI GGCC 4 cut(s) 398, 942, 1121, 1249
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 1 cut(s) 875
BsiSI CCGG 3 cut(s) 756, 966, 1250
BslFI GGGAC 2 cut(s) 270, 1136
BslI CCNNNNNNNGG 9 cut(s) 65, 316, 1045, 1129, 1207, 1282, 1320, 1377, 1378
BsmAI GTCTC 6 cut(s) 215, 338, 703, 806, 965, 1138
BsmFI GGGAC 2 cut(s) 270, 1136
BsmI GAATGC 1 cut(s) 11
BsnI GGCC 4 cut(s) 398, 942, 1121, 1249
Bso31I GGTCTC 3 cut(s) 703, 965, 1138
BsoBI CYCGRG 1 cut(s) 875
Bsp119I TTCGAA 1 cut(s) 159
Bsp120I GGGCCC 1 cut(s) 1119
Bsp1286I GDGCHC 6 cut(s) 518, 667, 1009, 1123, 1161, 1326
Bsp143I GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
Bsp19I CCATGG 2 cut(s) 538, 1009
BspACI CCGC 4 cut(s) 113, 147, 436, 1314
BspANI GGCC 4 cut(s) 398, 942, 1121, 1249
BspCNI CTCAG 5 cut(s) 64, 541, 613, 699, 1147
BspLI GGNNCC 5 cut(s) 321, 768, 1006, 1038, 1121
BspMI ACCTGC 1 cut(s) 947
BspPI GGATC 2 cut(s) 1192, 1207
BspT104I TTCGAA 1 cut(s) 159
BspTNI GGTCTC 3 cut(s) 703, 965, 1138
BsrDI GCAATG 1 cut(s) 1297
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 1 cut(s) 1195
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 7 cut(s) 393, 538, 583, 726, 1009, 1044, 1353
BssMI GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 4 cut(s) 393, 538, 1009, 1044
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 5 cut(s) 584, 728, 887, 1271, 1354
Bst4CI ACNGT 4 cut(s) 403, 736, 787, 817
BstBAI YACGTR 1 cut(s) 776
BstBI TTCGAA 1 cut(s) 159
BstC8I GCNNGC 4 cut(s) 307, 667, 1080, 1114
BstDEI CTNAG 7 cut(s) 72, 528, 621, 635, 707, 1155, 1389
BstDSI CCRYGG 2 cut(s) 538, 1009
BstEII GGTNACC 1 cut(s) 578
BstENI CCTNNNNNAGG 2 cut(s) 314, 1127
BstF5I GGATG 3 cut(s) 870, 895, 1342
BstH2I RGCGCY 1 cut(s) 559
BstHHI GCGC 2 cut(s) 558, 701
BstKTI GATC 6 cut(s) 201, 285, 387, 978, 1200, 1215
BstMAI GTCTC 6 cut(s) 215, 338, 703, 806, 965, 1138
BstMBI GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
BstMWI GCNNNNNNNGC 1 cut(s) 1118
BstNI CCWGG 5 cut(s) 584, 728, 887, 1271, 1354
BstNSI RCATGY 4 cut(s) 351, 435, 595, 857
BstPAI GACNNNNGTC 1 cut(s) 585
BstPI GGTNACC 1 cut(s) 578
BstSCI CCNGG 6 cut(s) 582, 726, 885, 1249, 1269, 1352
BstSFI CTRYAG 1 cut(s) 209
BstSLI GKGCMC 2 cut(s) 1123, 1326
BstV1I GCAGC 4 cut(s) 450, 520, 823, 1069
BstV2I GAAGAC 3 cut(s) 170, 902, 1313
BstX2I RGATCY 4 cut(s) 198, 282, 1197, 1212
BstXI CCANNNNNNTGG 2 cut(s) 1016, 1270
BstYI RGATCY 4 cut(s) 198, 282, 1197, 1212
BsuRI GGCC 4 cut(s) 398, 942, 1121, 1249
BtgI CCRYGG 2 cut(s) 538, 1009
BtsCI GGATG 3 cut(s) 870, 895, 1342
BtsI GCAGTG 1 cut(s) 300
BtsIMutI CAGTG 5 cut(s) 300, 399, 606, 672, 783
BveI ACCTGC 1 cut(s) 947
Cac8I GCNNGC 4 cut(s) 307, 667, 1080, 1114
CfoI GCGC 2 cut(s) 558, 701
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 7 cut(s) 319, 490, 941, 1036, 1119, 1120, 1273
CseI GACGC 1 cut(s) 978
Csp6I GTAC 2 cut(s) 693, 883
CspCI CAANNNNNGTGG 2 cut(s) 1267, 1302
CviQI GTAC 2 cut(s) 693, 883
DdeI CTNAG 7 cut(s) 72, 528, 621, 635, 707, 1155, 1389
DpnI GATC 6 cut(s) 200, 284, 386, 977, 1199, 1214
DpnII GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
DraI TTTAAA 1 cut(s) 45
DraIII CACNNNGTG 1 cut(s) 776
DrdI GACNNNNNNGTC 1 cut(s) 815
DseDI GACNNNNNNGTC 1 cut(s) 815
Eco130I CCWWGG 4 cut(s) 393, 538, 1009, 1044
Eco24I GRGCYC 4 cut(s) 518, 1009, 1123, 1161
Eco31I GGTCTC 3 cut(s) 703, 965, 1138
Eco47I GGWCC 4 cut(s) 319, 490, 1036, 1273
Eco57I CTGAAG 2 cut(s) 696, 1228
Eco88I CYCGRG 1 cut(s) 875
Eco91I GGTNACC 1 cut(s) 578
EcoNI CCTNNNNNAGG 2 cut(s) 314, 1127
EcoO109I RGGNCCY 5 cut(s) 319, 490, 1036, 1119, 1120
EcoO65I GGTNACC 1 cut(s) 578
EcoRII CCWGG 5 cut(s) 582, 726, 885, 1269, 1352
EcoT14I CCWWGG 4 cut(s) 393, 538, 1009, 1044
EcoT38I GRGCYC 4 cut(s) 518, 1009, 1123, 1161
ErhI CCWWGG 4 cut(s) 393, 538, 1009, 1044
FalI AAGNNNNNCTT 4 cut(s) 887, 919, 1220, 1252
FaqI GGGAC 2 cut(s) 270, 1136
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 6 cut(s) 436, 439, 509, 837, 1083, 1314
FokI GGATG 3 cut(s) 857, 902, 1349
FriOI GRGCYC 4 cut(s) 518, 1009, 1123, 1161
Fsp4HI GCNGC 6 cut(s) 436, 439, 509, 837, 1083, 1314
FspBI CTAG 1 cut(s) 494
FspI TGCGCA 1 cut(s) 700
GlaI GCGC 2 cut(s) 557, 700
GluI GCNGC 6 cut(s) 436, 439, 509, 837, 1083, 1314
GsaI CCCAGC 1 cut(s) 805
HaeII RGCGCY 1 cut(s) 559
HaeIII GGCC 4 cut(s) 398, 942, 1121, 1249
HapII CCGG 3 cut(s) 756, 966, 1250
HgaI GACGC 1 cut(s) 978
HhaI GCGC 2 cut(s) 558, 701
Hin6I GCGC 2 cut(s) 556, 699
HinP1I GCGC 2 cut(s) 556, 699
HincII GTYRAC 2 cut(s) 406, 499
HindII GTYRAC 2 cut(s) 406, 499
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 3 cut(s) 74, 242, 1299
HpaII CCGG 3 cut(s) 756, 966, 1250
HphI GGTGA 6 cut(s) 379, 590, 742, 955, 962, 1342
Hpy166II GTNNAC 5 cut(s) 250, 406, 499, 781, 1051
Hpy188I TCNGA 7 cut(s) 73, 198, 241, 289, 380, 457, 1331
Hpy188III TCNNGA 5 cut(s) 470, 1029, 1279, 1303, 1395
Hpy8I GTNNAC 5 cut(s) 250, 406, 499, 781, 1051
HpyAV CCTTC 3 cut(s) 65, 1222, 1326
HpyCH4III ACNGT 4 cut(s) 403, 736, 787, 817
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 6 cut(s) 11, 293, 351, 486, 650, 956
HpyF10VI GCNNNNNNNGC 1 cut(s) 1118
HpyF3I CTNAG 7 cut(s) 72, 528, 621, 635, 707, 1155, 1389
HpySE526I ACGT 1 cut(s) 775
HspAI GCGC 2 cut(s) 556, 699
Kzo9I GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
LmnI GCTCC 3 cut(s) 24, 850, 1004
Lsp1109I GCAGC 4 cut(s) 450, 520, 823, 1069
LweI GCATC 5 cut(s) 280, 292, 463, 618, 943
MaeI CTAG 1 cut(s) 494
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 6 cut(s) 425, 542, 578, 587, 730, 817
MalI GATC 6 cut(s) 200, 284, 386, 977, 1199, 1214
MboI GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
MboII GAAGA 7 cut(s) 170, 173, 427, 895, 907, 1308, 1318
MflI RGATCY 4 cut(s) 198, 282, 1197, 1212
MhlI GDGCHC 6 cut(s) 518, 667, 1009, 1123, 1161, 1326
MluCI AATT 1 cut(s) 1221
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 151
MseI TTAA 4 cut(s) 44, 861, 912, 1218
MslI CAYNNNNRTG 3 cut(s) 368, 951, 1014
MspI CCGG 3 cut(s) 756, 966, 1250
MspR9I CCNGG 6 cut(s) 584, 728, 887, 1251, 1271, 1354
Mva1269I GAATGC 1 cut(s) 11
MvaI CCWGG 5 cut(s) 584, 728, 887, 1271, 1354
MwoI GCNNNNNNNGC 1 cut(s) 1118
NciI CCSGG 1 cut(s) 1251
NcoI CCATGG 2 cut(s) 538, 1009
NdeII GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
NlaIV GGNNCC 5 cut(s) 321, 768, 1006, 1038, 1121
NmuCI GTSAC 6 cut(s) 425, 542, 578, 587, 730, 817
NsbI TGCGCA 1 cut(s) 700
NspI RCATGY 4 cut(s) 351, 435, 595, 857
NspV TTCGAA 1 cut(s) 159
PciI ACATGT 1 cut(s) 591
PctI GAATGC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 242, 1299
PkrI GCNGC 6 cut(s) 437, 440, 510, 838, 1084, 1315
PleI GAGTC 1 cut(s) 82
PpsI GAGTC 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 776
PpuMI RGGWCCY 3 cut(s) 319, 490, 1036
PscI ACATGT 1 cut(s) 591
PshAI GACNNNNGTC 1 cut(s) 585
Psp5II RGGWCCY 3 cut(s) 319, 490, 1036
Psp6I CCWGG 5 cut(s) 582, 726, 885, 1269, 1352
PspEI GGTNACC 1 cut(s) 578
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 5 cut(s) 582, 726, 885, 1269, 1352
PspN4I GGNNCC 5 cut(s) 321, 768, 1006, 1038, 1121
PspOMI GGGCCC 1 cut(s) 1119
PspPI GGNCC 7 cut(s) 319, 490, 941, 1036, 1119, 1120, 1273
PspPPI RGGWCCY 3 cut(s) 319, 490, 1036
PsuI RGATCY 4 cut(s) 198, 282, 1197, 1212
RsaI GTAC 2 cut(s) 694, 884
RsaNI GTAC 2 cut(s) 693, 883
RseI CAYNNNNRTG 3 cut(s) 368, 951, 1014
SaqAI TTAA 4 cut(s) 44, 861, 912, 1218
SatI GCNGC 6 cut(s) 436, 439, 509, 837, 1083, 1314
Sau3AI GATC 6 cut(s) 198, 282, 384, 975, 1197, 1212
Sau96I GGNCC 7 cut(s) 319, 490, 941, 1036, 1119, 1120, 1273
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 6 cut(s) 584, 728, 887, 1251, 1271, 1354
SduI GDGCHC 6 cut(s) 518, 667, 1009, 1123, 1161, 1326
SfaNI GCATC 5 cut(s) 280, 292, 463, 618, 943
SfcI CTRYAG 1 cut(s) 209
SfuI TTCGAA 1 cut(s) 159
SinI GGWCC 4 cut(s) 319, 490, 1036, 1273
SmiI ATTTAAAT 1 cut(s) 45
SmiMI CAYNNNNRTG 3 cut(s) 368, 951, 1014
SmlI CTYRAG 4 cut(s) 311, 845, 1277, 1373
SmoI CTYRAG 4 cut(s) 311, 845, 1277, 1373
Sse9I AATT 1 cut(s) 1221
SsiI CCGC 4 cut(s) 113, 147, 436, 1314
SspI AATATT 1 cut(s) 49
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 6 cut(s) 582, 726, 885, 1249, 1269, 1352
StyI CCWWGG 4 cut(s) 393, 538, 1009, 1044
SwaI ATTTAAAT 1 cut(s) 45
TaaI ACNGT 4 cut(s) 403, 736, 787, 817
TaiI ACGT 1 cut(s) 778
TaqI TCGA 2 cut(s) 159, 1176
TaqII GACCGA 2 cut(s) 455, 1156
TasI AATT 1 cut(s) 1221
TauI GCSGC 2 cut(s) 438, 1316
TfiI GAWTC 2 cut(s) 242, 1299
Tru1I TTAA 4 cut(s) 44, 861, 912, 1218
Tru9I TTAA 4 cut(s) 44, 861, 912, 1218
TscAI CASTG 5 cut(s) 300, 406, 613, 679, 790
TseFI GTSAC 6 cut(s) 425, 542, 578, 587, 730, 817
TseI GCWGC 4 cut(s) 438, 508, 836, 1082
Tsp45I GTSAC 6 cut(s) 425, 542, 578, 587, 730, 817
TspDTI ATGAA 3 cut(s) 4, 627, 765
TspGWI ACGGA 3 cut(s) 164, 413, 1200
TspRI CASTG 5 cut(s) 300, 406, 613, 679, 790
VpaK11BI GGWCC 4 cut(s) 319, 490, 1036, 1273
XagI CCTNNNNNAGG 2 cut(s) 314, 1127
XceI RCATGY 4 cut(s) 351, 435, 595, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 361
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.