RLG00000025170

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002178
Physical Location & Seq
Reverse (-)
42697021 .. 42699373
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025170

Sequence Viewer

Length: 1218 bp
ATGGACAAAACCTCAAAACTAAATCGGAGGCAAGTAAGCACTTCTAAGAAAATGGACACCTTCCTCTTCACCTCCGAGTCTGTGAATGAGGGACACCCCGATAAGCTCTGTGATCAGATTTCCGATGCAGTGCTTGATGCCTGCCTTGAACAAGATCCTGACAGCAAAGTTGCCTGTGAGACCTGCACCAAGACCAACATGGTCATGGTCTTCGGAGAGATCACAACTAAAGCCGAAGTGGACTATGAGAAGATAGTGCGCAACACCTGCCGCAGCATTGGATTTGTTTCGGATGATGTGGGCCTTGATGCTGAAAACTGCAAGGTCCTCGTGAACATTGAGCAACAAAGCCCTGATATAGCTCAGGGTGTGCATGGCCACCTCACCAAGAGGCCTGAGGAGATTGGTGCAGGAGACCAGGGTCACATGTTTGGTTATGCTACTGATGAGACCCCTGAGCTTATGCCTCTAAGCCATGTTCTTGCTACCAAGCTTGGTGCTCGTCTCACTGATGTTCGAAAGAATGGCACCTGCCCTTGGCTGAGACCTGATGGCAAGACTCAAGTCACTGTTGAGTACTACAATGACCATGGTGCTATGGTTCCAATCCGTGTCCACACTGTTCTCATTTCTACTCAGCATGATGAGACTGTTACCAATGACGAAATTGCAGCTGACCTCAAGAAACATGTCATTAAGCCTGTTGTTCCAGAGAAGTACCTAGATGAGAAGACTATTTTCCATCTTAACCCTTCAGGTCGATTTGTGATTGGTGGACCACATGGTGATGCAGGTCTAACTGGCCGAAAGATCATCATTGACACCTATGGTGGTTGGGGAGCACATGGCGGTGGTGCCTTCTCCGGTAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTAGCCAATGGACTTGCTAGAAGGTGCATAGTGCAGGTCTCATATGCAATTGGTGTACCTGAGCCACTATCGGTGTTTGTTGACACTTATGGGACTGGAAAGATTCCAGACAAGGAGATCCTGAAGTTAGTTAAGGAGGCCTTTGATTTTAGGCCTGGAATGATTGCCATTAACTTGGATCTCAAGAGAGGAAGGTATTTGAAAACGGCTGCTTATGGACACTTTGGTAGAGATGACCCTGACTTTTCGTGGGAAGTGGTGAAGTCCTTGAAGTGGGAAAAGGCGTAA

Protein Analysis

406

Amino Acids

44.58

Weight (kDa)

6.24

Isoelectric Point (pI)

26.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 21 - 118 2.4e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 134 - 255 3.6e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 257 - 395 8.8e-63 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 275, 539
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 5 cut(s) 191, 275, 539, 782, 955
AccB1I GGYRCC 3 cut(s) 527, 854, 896
AccI GTMKAC 1 cut(s) 885
AciI CCGC 2 cut(s) 271, 849
AclWI GGATC 3 cut(s) 149, 1042, 1116
AcoI YGGCCR 2 cut(s) 376, 802
AcuI CTGAAG 2 cut(s) 738, 1073
AdeI CACNNNGTG 1 cut(s) 785
AfaI GTAC 3 cut(s) 578, 719, 987
AfiI CCNNNNNNNGG 3 cut(s) 537, 880, 1203
AflIII ACRYGT 2 cut(s) 426, 688
AgsI TTSAA 3 cut(s) 149, 1132, 1201
AjnI CCWGG 2 cut(s) 417, 1084
AluBI AGCT 5 cut(s) 106, 362, 460, 493, 674
AluI AGCT 5 cut(s) 106, 362, 460, 493, 674
Alw21I GWGCWC 2 cut(s) 502, 844
Alw26I GTCTC 7 cut(s) 173, 408, 443, 509, 538, 641, 973
AlwI GGATC 3 cut(s) 149, 1042, 1116
AoxI GGCC 6 cut(s) 301, 376, 392, 802, 1068, 1082
ApeKI GCWGC 4 cut(s) 273, 671, 917, 1139
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 4 cut(s) 301, 325, 776, 871
AsuHPI GGTGA 4 cut(s) 61, 376, 797, 1201
AsuII TTCGAA 1 cut(s) 517
AvaII GGWCC 3 cut(s) 325, 776, 871
AxyI CCTNAGG 1 cut(s) 396
BalI TGGCCA 1 cut(s) 378
BanI GGYRCC 3 cut(s) 527, 854, 896
BauI CACGAG 1 cut(s) 329
BbsI GAAGAC 2 cut(s) 202, 737
Bbv12I GWGCWC 2 cut(s) 502, 844
BbvI GCAGC 4 cut(s) 285, 683, 904, 1126
BccI CCATC 2 cut(s) 545, 750
BceAI ACGGC 1 cut(s) 1152
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 2 cut(s) 419, 1086
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 7 cut(s) 173, 408, 443, 509, 538, 641, 973
BfaI CTAG 2 cut(s) 722, 948
BfuAI ACCTGC 5 cut(s) 191, 275, 539, 782, 955
BisI GCNGC 5 cut(s) 271, 274, 672, 918, 1140
BlsI GCNGC 5 cut(s) 272, 275, 673, 919, 1141
BmcAI AGTACT 1 cut(s) 578
Bme1390I CCNGG 2 cut(s) 419, 1086
Bme18I GGWCC 3 cut(s) 325, 776, 871
BmgT120I GGNCC 4 cut(s) 301, 325, 776, 871
BmiI GGNNCC 5 cut(s) 529, 603, 856, 873, 898
BmrFI CCNGG 2 cut(s) 419, 1086
BmsI GCATC 4 cut(s) 115, 127, 298, 778
BoxI GACNNNNGTC 2 cut(s) 420, 563
BpiI GAAGAC 2 cut(s) 202, 737
Bpu10I CCTNAGC 3 cut(s) 363, 456, 990
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 3 cut(s) 546, 665, 1097
BsaI GGTCTC 5 cut(s) 173, 408, 443, 538, 973
BsaJI CCNNGG 4 cut(s) 418, 536, 589, 879
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 3 cut(s) 537, 880, 1203
Bse1I ACTGG 2 cut(s) 805, 1030
Bse21I CCTNAGG 1 cut(s) 396
BseBI CCWGG 2 cut(s) 419, 1086
BseDI CCNNGG 4 cut(s) 418, 536, 589, 879
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 3 cut(s) 537, 880, 1203
BseMII CTCAG 6 cut(s) 377, 387, 447, 533, 650, 981
BseNI ACTGG 2 cut(s) 805, 1030
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 4 cut(s) 285, 683, 904, 1126
BsgI GTGCAG 3 cut(s) 169, 429, 983
BshFI GGCC 6 cut(s) 303, 378, 394, 804, 1070, 1084
BshNI GGYRCC 3 cut(s) 527, 854, 896
BsiHKAI GWGCWC 2 cut(s) 502, 844
BsiSI CCGG 1 cut(s) 864
BslFI GGGAC 2 cut(s) 105, 1036
BslI CCNNNNNNNGG 3 cut(s) 537, 880, 1203
BsmAI GTCTC 7 cut(s) 173, 408, 443, 509, 538, 641, 973
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 2 cut(s) 105, 1036
BsnI GGCC 6 cut(s) 303, 378, 394, 804, 1070, 1084
Bso31I GGTCTC 5 cut(s) 173, 408, 443, 538, 973
Bsp119I TTCGAA 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 502, 844
Bsp143I GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
Bsp19I CCATGG 1 cut(s) 589
BspACI CCGC 2 cut(s) 271, 849
BspANI GGCC 6 cut(s) 303, 378, 394, 804, 1070, 1084
BspCNI CTCAG 6 cut(s) 376, 388, 448, 534, 649, 982
BspLI GGNNCC 5 cut(s) 529, 603, 856, 873, 898
BspMI ACCTGC 5 cut(s) 191, 275, 539, 782, 955
BspPI GGATC 3 cut(s) 149, 1042, 1116
BspT104I TTCGAA 1 cut(s) 517
BspT107I GGYRCC 3 cut(s) 527, 854, 896
BspTNI GGTCTC 5 cut(s) 173, 408, 443, 538, 973
BsrI ACTGG 2 cut(s) 805, 1030
BssECI CCNNGG 4 cut(s) 418, 536, 589, 879
BssMI GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
BssSI CACGAG 1 cut(s) 329
BssT1I CCWWGG 3 cut(s) 536, 589, 879
Bst2BI CACGAG 1 cut(s) 329
Bst2UI CCWGG 2 cut(s) 419, 1086
Bst4CI ACNGT 3 cut(s) 571, 622, 652
Bst6I CTCTTC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 2 cut(s) 142, 915
BstDEI CTNAG 9 cut(s) 45, 363, 396, 456, 470, 542, 636, 921, 990
BstDSI CCRYGG 1 cut(s) 589
BstF5I GGATG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 6 cut(s) 115, 157, 222, 813, 1050, 1111
BstMAI GTCTC 7 cut(s) 173, 408, 443, 509, 538, 641, 973
BstMBI GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
BstMWI GCNNNNNNNGC 1 cut(s) 267
BstNI CCWGG 2 cut(s) 419, 1086
BstNSI RCATGY 2 cut(s) 430, 692
BstPAI GACNNNNGTC 2 cut(s) 420, 563
BstSCI CCNGG 2 cut(s) 417, 1084
BstV1I GCAGC 4 cut(s) 285, 683, 904, 1126
BstV2I GAAGAC 2 cut(s) 202, 737
BstX2I RGATCY 3 cut(s) 154, 1047, 1108
BstXI CCANNNNNNTGG 1 cut(s) 1105
BstYI RGATCY 3 cut(s) 154, 1047, 1108
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 6 cut(s) 303, 378, 394, 804, 1070, 1084
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 1 cut(s) 298
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 4 cut(s) 135, 507, 567, 618
BveI ACCTGC 5 cut(s) 191, 275, 539, 782, 955
Cac8I GCNNGC 2 cut(s) 142, 915
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 4 cut(s) 301, 325, 776, 871
Csp6I GTAC 3 cut(s) 577, 718, 986
CviQI GTAC 3 cut(s) 577, 718, 986
DdeI CTNAG 9 cut(s) 45, 363, 396, 456, 470, 542, 636, 921, 990
DpnI GATC 6 cut(s) 114, 156, 221, 812, 1049, 1110
DpnII GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
DraIII CACNNNGTG 1 cut(s) 785
EaeI YGGCCR 2 cut(s) 376, 802
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 3 cut(s) 536, 589, 879
Eco147I AGGCCT 3 cut(s) 394, 1070, 1084
Eco31I GGTCTC 5 cut(s) 173, 408, 443, 538, 973
Eco47I GGWCC 3 cut(s) 325, 776, 871
Eco57I CTGAAG 2 cut(s) 738, 1073
Eco81I CCTNAGG 1 cut(s) 396
EcoO109I RGGNCCY 2 cut(s) 325, 871
EcoRII CCWGG 2 cut(s) 417, 1084
EcoT14I CCWWGG 3 cut(s) 536, 589, 879
ErhI CCWWGG 3 cut(s) 536, 589, 879
Esp3I CGTCTC 1 cut(s) 509
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
FaqI GGGAC 2 cut(s) 105, 1036
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 5 cut(s) 271, 274, 672, 918, 1140
FokI GGATG 1 cut(s) 305
Fsp4HI GCNGC 5 cut(s) 271, 274, 672, 918, 1140
FspBI CTAG 2 cut(s) 722, 948
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 1 cut(s) 260
GluI GCNGC 5 cut(s) 271, 274, 672, 918, 1140
HaeIII GGCC 6 cut(s) 303, 378, 394, 804, 1070, 1084
HapII CCGG 1 cut(s) 864
HhaI GCGC 1 cut(s) 261
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 3 cut(s) 77, 559, 1033
HpaII CCGG 1 cut(s) 864
HphI GGTGA 4 cut(s) 61, 376, 797, 1201
Hpy166II GTNNAC 7 cut(s) 241, 334, 616, 776, 886, 986, 1012
Hpy188I TCNGA 6 cut(s) 27, 76, 117, 124, 215, 292
Hpy188III TCNNGA 7 cut(s) 158, 331, 682, 710, 1037, 1051, 1114
Hpy8I GTNNAC 7 cut(s) 241, 334, 616, 776, 886, 986, 1012
HpyAV CCTTC 5 cut(s) 70, 762, 868, 945, 1116
HpyCH4III ACNGT 3 cut(s) 571, 622, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 267
HpyF3I CTNAG 9 cut(s) 45, 363, 396, 456, 470, 542, 636, 921, 990
HspAI GCGC 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
LmnI GCTCC 1 cut(s) 839
Lsp1109I GCAGC 4 cut(s) 285, 683, 904, 1126
LweI GCATC 4 cut(s) 115, 127, 298, 778
MaeI CTAG 2 cut(s) 722, 948
MaeIII GTNAC 3 cut(s) 422, 565, 652
MalI GATC 6 cut(s) 114, 156, 221, 812, 1049, 1110
MboI GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
MboII GAAGA 4 cut(s) 58, 202, 262, 742
MfeI CAATTG 1 cut(s) 978
MflI RGATCY 3 cut(s) 154, 1047, 1108
MhlI GDGCHC 2 cut(s) 502, 844
MlsI TGGCCA 1 cut(s) 378
MluCI AATT 2 cut(s) 666, 978
MluNI TGGCCA 1 cut(s) 378
MlyI GAGTC 2 cut(s) 86, 553
Mox20I TGGCCA 1 cut(s) 378
MscI TGGCCA 1 cut(s) 378
MseI TTAA 4 cut(s) 696, 747, 1062, 1101
MslI CAYNNNNRTG 3 cut(s) 203, 786, 849
Msp20I TGGCCA 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 1 cut(s) 864
MspR9I CCNGG 2 cut(s) 419, 1086
MunI CAATTG 1 cut(s) 978
MvaI CCWGG 2 cut(s) 419, 1086
MwoI GCNNNNNNNGC 1 cut(s) 267
NcoI CCATGG 1 cut(s) 589
NdeI CATATG 1 cut(s) 973
NdeII GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
NlaIV GGNNCC 5 cut(s) 529, 603, 856, 873, 898
NmuCI GTSAC 2 cut(s) 422, 565
NsbI TGCGCA 1 cut(s) 260
NspI RCATGY 2 cut(s) 430, 692
NspV TTCGAA 1 cut(s) 517
PaqCI CACCTGC 2 cut(s) 275, 539
PceI AGGCCT 3 cut(s) 394, 1070, 1084
PciI ACATGT 2 cut(s) 426, 688
PfeI GAWTC 1 cut(s) 1033
PkrI GCNGC 5 cut(s) 272, 275, 673, 919, 1141
PleI GAGTC 2 cut(s) 85, 553
PpsI GAGTC 2 cut(s) 85, 553
PpuMI RGGWCCY 2 cut(s) 325, 871
PscI ACATGT 2 cut(s) 426, 688
PshAI GACNNNNGTC 2 cut(s) 420, 563
Psp5II RGGWCCY 2 cut(s) 325, 871
Psp6I CCWGG 2 cut(s) 417, 1084
PspGI CCWGG 2 cut(s) 417, 1084
PspN4I GGNNCC 5 cut(s) 529, 603, 856, 873, 898
PspPI GGNCC 4 cut(s) 301, 325, 776, 871
PspPPI RGGWCCY 2 cut(s) 325, 871
PsuI RGATCY 3 cut(s) 154, 1047, 1108
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 3 cut(s) 578, 719, 987
RsaNI GTAC 3 cut(s) 577, 718, 986
RseI CAYNNNNRTG 3 cut(s) 203, 786, 849
SaqAI TTAA 4 cut(s) 696, 747, 1062, 1101
SatI GCNGC 5 cut(s) 271, 274, 672, 918, 1140
Sau3AI GATC 6 cut(s) 112, 154, 219, 810, 1047, 1108
Sau96I GGNCC 4 cut(s) 301, 325, 776, 871
ScaI AGTACT 1 cut(s) 578
SchI GAGTC 2 cut(s) 86, 553
ScrFI CCNGG 2 cut(s) 419, 1086
SduI GDGCHC 2 cut(s) 502, 844
SfaNI GCATC 4 cut(s) 115, 127, 298, 778
SfuI TTCGAA 1 cut(s) 517
SinI GGWCC 3 cut(s) 325, 776, 871
SmiMI CAYNNNNRTG 3 cut(s) 203, 786, 849
SmlI CTYRAG 3 cut(s) 561, 680, 1112
SmoI CTYRAG 3 cut(s) 561, 680, 1112
Sse9I AATT 2 cut(s) 666, 978
SseBI AGGCCT 3 cut(s) 394, 1070, 1084
SsiI CCGC 2 cut(s) 271, 849
SspMI CTAG 2 cut(s) 722, 948
StuI AGGCCT 3 cut(s) 394, 1070, 1084
StyD4I CCNGG 2 cut(s) 417, 1084
StyI CCWWGG 3 cut(s) 536, 589, 879
TaaI ACNGT 3 cut(s) 571, 622, 652
TaqI TCGA 2 cut(s) 517, 760
TasI AATT 2 cut(s) 666, 978
TatI WGTACW 1 cut(s) 576
TauI GCSGC 1 cut(s) 273
TfiI GAWTC 1 cut(s) 1033
Tru1I TTAA 4 cut(s) 696, 747, 1062, 1101
Tru9I TTAA 4 cut(s) 696, 747, 1062, 1101
TscAI CASTG 4 cut(s) 135, 514, 574, 625
TseFI GTSAC 2 cut(s) 422, 565
TseI GCWGC 4 cut(s) 273, 671, 917, 1139
Tsp45I GTSAC 2 cut(s) 422, 565
TspGWI ACGGA 1 cut(s) 599
TspRI CASTG 4 cut(s) 135, 514, 574, 625
VpaK11BI GGWCC 3 cut(s) 325, 776, 871
XceI RCATGY 2 cut(s) 430, 692
XcmI CCANNNNNNNNNTGG 2 cut(s) 196, 202
XmiI GTMKAC 1 cut(s) 885
XspI CTAG 2 cut(s) 722, 948
ZrmI AGTACT 1 cut(s) 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.