MD12G1201100.v1.1

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002178
Physical Location & Seq
Reverse (-)
28171257 .. 28173526
2270 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1201100.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGCTTGTAAGAATGGAAGACACCTTCCTGTTTACTTCGGAGTCCGTGAACGAGGGACACCCCGATAAACTCTGTGATCAGATTTCTGATGCGGTTCTCGACGCCTGCCTCGAACAAGACCCCGACAGCAAAGTTGCCTGCGAGACTTGCACCAAGACGAACATGGTCATGGTCTTCGGAGAGATTACAACCAAAGCCAAAGTGGACTACGAGAAGATTGTGCGCGATACCTGCCGTACAATTGGATTTGTTTCAGACAATGTTGGTCTTGATGCTGACAACTGCAAGGTCTTGGTTAACATCGAGCAACAGAGCCCTGATATCGCGCAGGGCGTCCATGGCCACTTGACCAAGCGGCCTGAGGAGATTGGTGCGGGTGACCAGGGTCACATGTTTGGTTATGCAACTGATGAGACCCCTGAGCTTATGCCTCTGAGCCATGTTCTTGCCACCAAGCTTGGTGCCCGTCTCACTGAAGTTCGCAAGAATGGAACCTTGCCGTGGCTGAGACCCGATGGCAAGACGCAAGTCACTGTTGAATACTACAATGACCATGGCGCTATGGTTCCAATCCGTGTCCACACGGTTCTCATTTCAACTCAGCATGACGAAACTGTTACCAACGACGAGATTGCTGCTGACCTCAAAGAGCATGTTATCAAGCCTGTTGTTCCTGAGAAGTACCTAGACGAGAAGACTATTTTCCATCTTAACCCATCAGGTCGATTTGTCATTGGTGGACCTCACGGTGATGCTGGCCTCACTGGCCGAAAGATCATCATTGACACCTACGGTGGTTGGGGAGCTCATGGTGGTGGTGCCTTCTCCGGCAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTCGCCAATGGCCTTGCCCGCAGGTGCATAGTGCAGGTCTCGTATGCCATTGGTGTTCCCGAGCCACTATCAGTGTTCGTTGACAGTTATGGCACCGGAAAGATTCCTGACAAGGAGATTCTTAAACTAGTAAAGGAGAACTTTGATTTCAGGCCTGGGATGATTGCGATTAACCTCGATCTCAAGCGGGGCAGGTACTTGAAAACGGCTGCGTATGGACACTTCGGTAGGGATGACCCTGACTTCTCATGGGAAGTGGTGAAGCCATTGAAGTGGGAAAAGCCCCAAGAGTAG

Protein Analysis

396

Amino Acids

43.56

Weight (kDa)

5.67

Isoelectric Point (pI)

24.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 9 - 106 2.5e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 122 - 243 7.3e-48 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 245 - 383 1.1e-62 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 906
Acc36I ACCTGC 4 cut(s) 239, 906, 919, 1077
AccB1I GGYRCC 4 cut(s) 461, 818, 860, 986
AccI GTMKAC 1 cut(s) 849
AccII CGCG 2 cut(s) 225, 326
AciI CCGC 5 cut(s) 92, 355, 374, 913, 1081
AcoI YGGCCR 2 cut(s) 340, 766
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 102, 333
AfaI GTAC 3 cut(s) 238, 683, 1091
AfiI CCNNNNNNNGG 2 cut(s) 501, 844
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 4 cut(s) 539, 597, 1096, 1165
AhlI ACTAGT 1 cut(s) 1021
AjnI CCWGG 2 cut(s) 381, 1048
AluBI AGCT 3 cut(s) 424, 457, 806
AluI AGCT 3 cut(s) 424, 457, 806
Alw21I GWGCWC 1 cut(s) 808
Alw26I GTCTC 5 cut(s) 137, 407, 473, 502, 937
Ama87I CYCGRG 1 cut(s) 953
AoxI GGCC 6 cut(s) 340, 356, 757, 766, 904, 1046
ApeKI GCWGC 3 cut(s) 635, 881, 1103
AspLEI GCGC 3 cut(s) 225, 328, 560
AspS9I GGNCC 2 cut(s) 740, 835
AsuHPI GGTGA 3 cut(s) 389, 761, 1165
AvaI CYCGRG 1 cut(s) 953
AvaII GGWCC 2 cut(s) 740, 835
AxyI CCTNAGG 1 cut(s) 360
BaeGI GKGCMC 1 cut(s) 466
BalI TGGCCA 1 cut(s) 342
BanI GGYRCC 4 cut(s) 461, 818, 860, 986
BanII GRGCYC 2 cut(s) 317, 808
BarI GAAGNNNNNNTAC 2 cut(s) 1100, 1132
BbsI GAAGAC 3 cut(s) 24, 166, 701
Bbv12I GWGCWC 1 cut(s) 808
BbvI GCAGC 3 cut(s) 622, 868, 1090
BccI CCATC 3 cut(s) 509, 714, 724
BceAI ACGGC 3 cut(s) 219, 484, 1116
BcgI CGANNNNNNTGC 4 cut(s) 614, 617, 648, 651
BciT130I CCWGG 2 cut(s) 383, 1050
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 5 cut(s) 137, 407, 473, 502, 937
BcuI ACTAGT 1 cut(s) 1021
BfaI CTAG 2 cut(s) 686, 1022
BfoI RGCGCY 1 cut(s) 561
BfuAI ACCTGC 4 cut(s) 239, 906, 919, 1077
BglI GCCNNNNNGGC 1 cut(s) 765
BisI GCNGC 4 cut(s) 356, 636, 882, 1104
BlsI GCNGC 4 cut(s) 357, 637, 883, 1105
Bme1390I CCNGG 2 cut(s) 383, 1050
Bme18I GGWCC 2 cut(s) 740, 835
BmeT110I CYCGRG 1 cut(s) 953
BmgT120I GGNCC 2 cut(s) 740, 835
BmiI GGNNCC 7 cut(s) 463, 493, 567, 820, 837, 862, 988
BmrFI CCNGG 2 cut(s) 383, 1050
BmsI GCATC 3 cut(s) 79, 262, 742
BoxI GACNNNNGTC 2 cut(s) 384, 527
BpiI GAAGAC 3 cut(s) 24, 166, 701
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 1061
BsaHI GRCGYC 2 cut(s) 102, 333
BsaI GGTCTC 3 cut(s) 407, 502, 937
BsaJI CCNNGG 6 cut(s) 337, 382, 500, 553, 843, 1049
BsaWI WCCGGW 1 cut(s) 989
Bsc4I CCNNNNNNNGG 2 cut(s) 501, 844
Bse1I ACTGG 1 cut(s) 769
Bse21I CCTNAGG 1 cut(s) 360
BseBI CCWGG 2 cut(s) 383, 1050
BseDI CCNNGG 6 cut(s) 337, 382, 500, 553, 843, 1049
BseGI GGATG 2 cut(s) 1059, 1132
BseLI CCNNNNNNNGG 2 cut(s) 501, 844
BseMII CTCAG 6 cut(s) 351, 411, 425, 497, 614, 666
BseNI ACTGG 1 cut(s) 769
BseRI GAGGAG 1 cut(s) 377
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 3 cut(s) 622, 868, 1090
BsgI GTGCAG 1 cut(s) 947
Bsh1236I CGCG 2 cut(s) 225, 326
BshFI GGCC 6 cut(s) 342, 358, 759, 768, 906, 1048
BshNI GGYRCC 4 cut(s) 461, 818, 860, 986
BsiHKAI GWGCWC 1 cut(s) 808
BsiHKCI CYCGRG 1 cut(s) 953
BsiSI CCGG 2 cut(s) 828, 990
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 501, 844
BsmAI GTCTC 5 cut(s) 137, 407, 473, 502, 937
BsmBI CGTCTC 1 cut(s) 473
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 6 cut(s) 342, 358, 759, 768, 906, 1048
Bso31I GGTCTC 3 cut(s) 407, 502, 937
BsoBI CYCGRG 1 cut(s) 953
Bsp1286I GDGCHC 3 cut(s) 317, 466, 808
Bsp143I GATC 3 cut(s) 76, 774, 1072
Bsp19I CCATGG 2 cut(s) 337, 553
BspACI CCGC 5 cut(s) 92, 355, 374, 913, 1081
BspANI GGCC 6 cut(s) 342, 358, 759, 768, 906, 1048
BspCNI CTCAG 6 cut(s) 352, 412, 426, 498, 613, 667
BspFNI CGCG 2 cut(s) 225, 326
BspLI GGNNCC 7 cut(s) 463, 493, 567, 820, 837, 862, 988
BspMI ACCTGC 4 cut(s) 239, 906, 919, 1077
BspT107I GGYRCC 4 cut(s) 461, 818, 860, 986
BspTNI GGTCTC 3 cut(s) 407, 502, 937
BsrI ACTGG 1 cut(s) 769
BssECI CCNNGG 6 cut(s) 337, 382, 500, 553, 843, 1049
BssMI GATC 3 cut(s) 76, 774, 1072
BssNI GRCGYC 2 cut(s) 102, 333
BssT1I CCWWGG 3 cut(s) 337, 553, 843
Bst2UI CCWGG 2 cut(s) 383, 1050
Bst4CI ACNGT 6 cut(s) 535, 586, 616, 749, 794, 980
BstACI GRCGYC 2 cut(s) 102, 333
BstC8I GCNNGC 5 cut(s) 106, 139, 757, 879, 913
BstDEI CTNAG 7 cut(s) 360, 420, 434, 506, 600, 675, 885
BstDSI CCRYGG 3 cut(s) 337, 500, 553
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 2 cut(s) 1059, 1132
BstFNI CGCG 2 cut(s) 225, 326
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 3 cut(s) 225, 328, 560
BstKTI GATC 3 cut(s) 79, 777, 1075
BstMAI GTCTC 5 cut(s) 137, 407, 473, 502, 937
BstMBI GATC 3 cut(s) 76, 774, 1072
BstMWI GCNNNNNNNGC 5 cut(s) 147, 231, 339, 765, 912
BstNI CCWGG 2 cut(s) 383, 1050
BstNSI RCATGY 2 cut(s) 394, 656
BstPAI GACNNNNGTC 2 cut(s) 384, 527
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 381, 1048
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 2 cut(s) 225, 326
BstV1I GCAGC 3 cut(s) 622, 868, 1090
BstV2I GAAGAC 3 cut(s) 24, 166, 701
BstXI CCANNNNNNTGG 1 cut(s) 1167
Bsu36I CCTNAGG 1 cut(s) 360
BsuRI GGCC 6 cut(s) 342, 358, 759, 768, 906, 1048
BtgI CCRYGG 3 cut(s) 337, 500, 553
BtsCI GGATG 2 cut(s) 1059, 1132
BtsIMutI CAGTG 4 cut(s) 471, 531, 762, 972
BveI ACCTGC 4 cut(s) 239, 906, 919, 1077
Cac8I GCNNGC 5 cut(s) 106, 139, 757, 879, 913
CfoI GCGC 3 cut(s) 225, 328, 560
Cfr13I GGNCC 2 cut(s) 740, 835
CseI GACGC 3 cut(s) 110, 322, 532
Csp6I GTAC 3 cut(s) 237, 682, 1090
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviQI GTAC 3 cut(s) 237, 682, 1090
DdeI CTNAG 7 cut(s) 360, 420, 434, 506, 600, 675, 885
DpnI GATC 3 cut(s) 78, 776, 1074
DpnII GATC 3 cut(s) 76, 774, 1072
EaeI YGGCCR 2 cut(s) 340, 766
Ecl136II GAGCTC 1 cut(s) 806
Eco130I CCWWGG 3 cut(s) 337, 553, 843
Eco147I AGGCCT 1 cut(s) 1048
Eco24I GRGCYC 2 cut(s) 317, 808
Eco31I GGTCTC 3 cut(s) 407, 502, 937
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 740, 835
Eco53kI GAGCTC 1 cut(s) 806
Eco57I CTGAAG 1 cut(s) 495
Eco81I CCTNAGG 1 cut(s) 360
Eco88I CYCGRG 1 cut(s) 953
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 806
EcoO109I RGGNCCY 1 cut(s) 835
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 2 cut(s) 381, 1048
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 3 cut(s) 337, 553, 843
EcoT38I GRGCYC 2 cut(s) 317, 808
ErhI CCWWGG 3 cut(s) 337, 553, 843
Esp3I CGTCTC 1 cut(s) 473
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 1 cut(s) 69
FauI CCCGC 3 cut(s) 367, 920, 1074
FbaI TGATCA 1 cut(s) 76
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 4 cut(s) 356, 636, 882, 1104
FokI GGATG 2 cut(s) 1066, 1139
FriOI GRGCYC 2 cut(s) 317, 808
Fsp4HI GCNGC 4 cut(s) 356, 636, 882, 1104
FspBI CTAG 2 cut(s) 686, 1022
GlaI GCGC 3 cut(s) 224, 327, 559
GluI GCNGC 4 cut(s) 356, 636, 882, 1104
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 6 cut(s) 342, 358, 759, 768, 906, 1048
HapII CCGG 2 cut(s) 828, 990
HgaI GACGC 3 cut(s) 110, 322, 532
HhaI GCGC 3 cut(s) 225, 328, 560
Hin1I GRCGYC 2 cut(s) 102, 333
Hin6I GCGC 3 cut(s) 223, 326, 558
HinP1I GCGC 3 cut(s) 223, 326, 558
HincII GTYRAC 2 cut(s) 298, 976
HindII GTYRAC 2 cut(s) 298, 976
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 3 cut(s) 41, 997, 1012
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 2 cut(s) 828, 990
HphI GGTGA 3 cut(s) 389, 761, 1165
Hpy166II GTNNAC 8 cut(s) 33, 49, 205, 298, 580, 740, 850, 976
Hpy188I TCNGA 6 cut(s) 40, 81, 88, 179, 256, 435
Hpy188III TCNNGA 5 cut(s) 98, 269, 674, 953, 1001
Hpy8I GTNNAC 8 cut(s) 33, 49, 205, 298, 580, 740, 850, 976
Hpy99I CGWCG 2 cut(s) 104, 629
HpyAV CCTTC 2 cut(s) 34, 832
HpyCH4III ACNGT 6 cut(s) 535, 586, 616, 749, 794, 980
HpyCH4V TGCA 5 cut(s) 150, 285, 404, 921, 928
HpyF10VI GCNNNNNNNGC 5 cut(s) 147, 231, 339, 765, 912
HpyF3I CTNAG 7 cut(s) 360, 420, 434, 506, 600, 675, 885
Hsp92I GRCGYC 2 cut(s) 102, 333
HspAI GCGC 3 cut(s) 223, 326, 558
Ksp22I TGATCA 1 cut(s) 76
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 76, 774, 1072
LmnI GCTCC 1 cut(s) 803
Lsp1109I GCAGC 3 cut(s) 622, 868, 1090
LweI GCATC 3 cut(s) 79, 262, 742
MaeI CTAG 2 cut(s) 686, 1022
MaeIII GTNAC 4 cut(s) 377, 386, 529, 616
MalI GATC 3 cut(s) 78, 776, 1074
MboI GATC 3 cut(s) 76, 774, 1072
MboII GAAGA 4 cut(s) 29, 166, 226, 706
MfeI CAATTG 1 cut(s) 240
MhlI GDGCHC 3 cut(s) 317, 466, 808
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 1 cut(s) 240
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 8 cut(s) 46, 119, 355, 441, 653, 753, 770, 1079
Mox20I TGGCCA 1 cut(s) 342
MscI TGGCCA 1 cut(s) 342
MseI TTAA 4 cut(s) 297, 711, 1017, 1065
MslI CAYNNNNRTG 4 cut(s) 167, 750, 813, 1165
Msp20I TGGCCA 1 cut(s) 342
MspI CCGG 2 cut(s) 828, 990
MspR9I CCNGG 2 cut(s) 383, 1050
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 2 cut(s) 383, 1050
MvnI CGCG 2 cut(s) 225, 326
MwoI GCNNNNNNNGC 5 cut(s) 147, 231, 339, 765, 912
NcoI CCATGG 2 cut(s) 337, 553
NdeII GATC 3 cut(s) 76, 774, 1072
NlaIV GGNNCC 7 cut(s) 463, 493, 567, 820, 837, 862, 988
NmuCI GTSAC 3 cut(s) 377, 386, 529
NspI RCATGY 2 cut(s) 394, 656
PaqCI CACCTGC 1 cut(s) 906
PceI AGGCCT 1 cut(s) 1048
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 330
PfeI GAWTC 2 cut(s) 997, 1012
PkrI GCNGC 4 cut(s) 357, 637, 883, 1105
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PpuMI RGGWCCY 1 cut(s) 835
PscI ACATGT 1 cut(s) 390
PshAI GACNNNNGTC 2 cut(s) 384, 527
Psp124BI GAGCTC 1 cut(s) 808
Psp5II RGGWCCY 1 cut(s) 835
Psp6I CCWGG 2 cut(s) 381, 1048
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 2 cut(s) 381, 1048
PspN4I GGNNCC 7 cut(s) 463, 493, 567, 820, 837, 862, 988
PspPI GGNCC 2 cut(s) 740, 835
PspPPI RGGWCCY 1 cut(s) 835
RsaI GTAC 3 cut(s) 238, 683, 1091
RsaNI GTAC 3 cut(s) 237, 682, 1090
RseI CAYNNNNRTG 4 cut(s) 167, 750, 813, 1165
SacI GAGCTC 1 cut(s) 808
SaqAI TTAA 4 cut(s) 297, 711, 1017, 1065
SatI GCNGC 4 cut(s) 356, 636, 882, 1104
Sau3AI GATC 3 cut(s) 76, 774, 1072
Sau96I GGNCC 2 cut(s) 740, 835
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 2 cut(s) 383, 1050
SduI GDGCHC 3 cut(s) 317, 466, 808
SfaNI GCATC 3 cut(s) 79, 262, 742
SfiI GGCCNNNNNGGCC 1 cut(s) 765
SinI GGWCC 2 cut(s) 740, 835
SmiMI CAYNNNNRTG 4 cut(s) 167, 750, 813, 1165
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
SpeI ACTAGT 1 cut(s) 1021
Sse9I AATT 1 cut(s) 240
SseBI AGGCCT 1 cut(s) 1048
SsiI CCGC 5 cut(s) 92, 355, 374, 913, 1081
SspMI CTAG 2 cut(s) 686, 1022
SstI GAGCTC 1 cut(s) 808
StuI AGGCCT 1 cut(s) 1048
StyD4I CCNGG 2 cut(s) 381, 1048
StyI CCWWGG 3 cut(s) 337, 553, 843
TaaI ACNGT 6 cut(s) 535, 586, 616, 749, 794, 980
TaqI TCGA 5 cut(s) 99, 111, 303, 724, 1071
TasI AATT 1 cut(s) 240
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 2 cut(s) 997, 1012
Tru1I TTAA 4 cut(s) 297, 711, 1017, 1065
Tru9I TTAA 4 cut(s) 297, 711, 1017, 1065
TscAI CASTG 4 cut(s) 478, 538, 769, 972
TseFI GTSAC 3 cut(s) 377, 386, 529
TseI GCWGC 3 cut(s) 635, 881, 1103
Tsp45I GTSAC 3 cut(s) 377, 386, 529
TspGWI ACGGA 2 cut(s) 34, 563
TspRI CASTG 4 cut(s) 478, 538, 769, 972
VpaK11BI GGWCC 2 cut(s) 740, 835
XceI RCATGY 2 cut(s) 394, 656
XcmI CCANNNNNNNNNTGG 2 cut(s) 160, 199
XmiI GTMKAC 1 cut(s) 849
XspI CTAG 2 cut(s) 686, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.