pycom09g19680

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002178
Physical Location & Seq
Forward (+)
20532661 .. 20533698
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19680.1

Sequence Viewer

Length: 1038 bp
ATGGTCATGGTGTTCGGAGAGATCACTACCAAGGCCAATGTTGACTATGAGAAGATAGTCCGTGACACTTGCCGCGCCATTGGATTTGTTTCTGATGATGTGGGTCTTGATGCCGATAACTGCAAGGTCCTGGTTAATATTGAGCAACAGAGCCCTGATATTGCCCAGGGTGTCCATGGTCACTTTACCAAGCGACCTGAGGAGATTGGTGCTGGTGACCAGGGTCACATGTTTGGTTATGCCACTGATGAAACCCCTGAATTGATGCCCCTTAGCCATGTGCTTTCCACCAAGCTTGGTGCTCGTCTCACTGAGGTTAGGAAGAATGGTACCTGCCCTTGGTTGAGACCTGATGGCAAGACCCAAGTCACCGTGGAGTACTACAACGAAAATGGAGCTATGGTTCCAGTCCGTGTCCACACTGTTCTCATCTCCACCCAGCATGATGAGACTGTCACAAACGATGAGATTGCTGCTGACCTTAAGGAGCATGTCATTAAGCCTGTTGTCCCGGAGAAGTACCTTGACGAGAAGACAATCTTCCACCTTAACCCCTCAGGCCGCTTTGTGATTGGTGGTCCTCATGGTGATGCAGGTCTTACCGGACGCAAGATTATTATTGACACTTATGGTGGATGGGGAGCCCATGGTGGAGGTGCTTTCTCTGGAAAGGACCCAACCAAGGTCGACAGGAGTGGCGCCTACATTGTGAGGCAGGCTGCCAAGAGCATTGTAGCAAATGGGCTTGCTCGCAGGGCCATTGTGCAGGTGTCTTATGCCATCGGTGTGCCGGAGCCCTTGTCAGTTTTCGTTGACACATATGGAACTGGAAAGATCGATCCTGACAAGGAAATCCTTAAAATTGTGAAGGAGACCTTCGACTTCAGGCCTGGCATGATCACTATCAACCTGGACCTCAAGAGGGGTGGTGGCGGAAGGTTCTTGAAGACAGCTGCCTATGGACACTTTGGAAGGGACGATCCAGATTTCACCTGGGAGGTTGTGAAGCCCCTCAAGTGGGAGAAGCCTCAATCTTGA

Protein Analysis

346

Amino Acids

37.8

Weight (kDa)

6.14

Isoelectric Point (pI)

21.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 1 - 52 4.6e-12 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 68 - 189 7.4e-48 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 191 - 333 2.7e-59 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 757
Acc36I ACCTGC 3 cut(s) 341, 584, 757
Acc65I GGTACC 1 cut(s) 329
AccB1I GGYRCC 2 cut(s) 329, 698
AccI GTMKAC 1 cut(s) 687
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 73, 562, 933
AclWI GGATC 2 cut(s) 833, 974
AcuI CTGAAG 1 cut(s) 868
AcyI GRCGYC 1 cut(s) 699
AfaI GTAC 3 cut(s) 331, 380, 521
AfiI CCNNNNNNNGG 5 cut(s) 339, 682, 922, 1017, 1018
AflII CTTAAG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 1 cut(s) 946
AjnI CCWGG 6 cut(s) 129, 165, 219, 889, 909, 992
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 3 cut(s) 295, 398, 953
AluI AGCT 3 cut(s) 295, 398, 953
Alw21I GWGCWC 1 cut(s) 304
Alw26I GTCTC 4 cut(s) 311, 340, 443, 866
AlwI GGATC 2 cut(s) 833, 974
AoxI GGCC 4 cut(s) 33, 559, 756, 887
ApeKI GCWGC 3 cut(s) 473, 719, 953
Asp718I GGTACC 1 cut(s) 329
AspLEI GCGC 2 cut(s) 77, 701
AspS9I GGNCC 5 cut(s) 127, 578, 673, 756, 913
AsuC2I CCSGG 1 cut(s) 512
AsuHPI GGTGA 4 cut(s) 227, 361, 599, 982
AvaII GGWCC 4 cut(s) 127, 578, 673, 913
AxyI CCTNAGG 2 cut(s) 198, 556
BanI GGYRCC 2 cut(s) 329, 698
BanII GRGCYC 3 cut(s) 155, 646, 798
BbsI GAAGAC 2 cut(s) 539, 953
Bbv12I GWGCWC 1 cut(s) 304
BbvI GCAGC 3 cut(s) 460, 706, 940
BccI CCATC 3 cut(s) 347, 630, 788
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 6 cut(s) 131, 167, 221, 891, 911, 994
BclI TGATCA 1 cut(s) 897
BcnI CCSGG 1 cut(s) 512
BcoDI GTCTC 4 cut(s) 311, 340, 443, 866
BfoI RGCGCY 1 cut(s) 702
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 3 cut(s) 341, 584, 757
BisI GCNGC 5 cut(s) 73, 474, 562, 720, 954
BlsI GCNGC 5 cut(s) 74, 475, 563, 721, 955
BmcAI AGTACT 1 cut(s) 380
Bme1390I CCNGG 7 cut(s) 131, 167, 221, 512, 891, 911, 994
Bme18I GGWCC 4 cut(s) 127, 578, 673, 913
BmgT120I GGNCC 5 cut(s) 127, 578, 673, 756, 913
BmiI GGNNCC 6 cut(s) 331, 405, 643, 675, 700, 795
BmrFI CCNGG 7 cut(s) 131, 167, 221, 512, 891, 911, 994
BmsI GCATC 3 cut(s) 100, 255, 580
BoxI GACNNNNGTC 2 cut(s) 222, 365
BpiI GAAGAC 2 cut(s) 539, 953
Bpu10I CCTNAGC 1 cut(s) 272
BpuEI CTTGAG 2 cut(s) 902, 998
BpuMI CCSGG 1 cut(s) 512
Bsa29I ATCGAT 1 cut(s) 837
BsaBI GATNNNNATC 1 cut(s) 902
BsaHI GRCGYC 1 cut(s) 699
BsaI GGTCTC 2 cut(s) 340, 866
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 4 cut(s) 387, 417, 785, 815
Bsc4I CCNNNNNNNGG 5 cut(s) 339, 682, 922, 1017, 1018
Bse1I ACTGG 2 cut(s) 407, 832
Bse21I CCTNAGG 2 cut(s) 198, 556
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 6 cut(s) 131, 167, 221, 891, 911, 994
BseCI ATCGAT 1 cut(s) 837
BseGI GGATG 1 cut(s) 641
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 5 cut(s) 339, 682, 922, 1017, 1018
BseMII CTCAG 3 cut(s) 189, 303, 570
BseNI ACTGG 2 cut(s) 407, 832
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 3 cut(s) 460, 706, 940
BseYI CCCAGC 1 cut(s) 438
BsgI GTGCAG 1 cut(s) 785
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 4 cut(s) 35, 561, 758, 889
BshNI GGYRCC 2 cut(s) 329, 698
BshVI ATCGAT 1 cut(s) 837
BsiHKAI GWGCWC 1 cut(s) 304
BsiSI CCGG 3 cut(s) 512, 603, 791
BslFI GGGAC 2 cut(s) 494, 989
BslI CCNNNNNNNGG 5 cut(s) 339, 682, 922, 1017, 1018
BsmAI GTCTC 4 cut(s) 311, 340, 443, 866
BsmBI CGTCTC 1 cut(s) 311
BsmFI GGGAC 2 cut(s) 494, 989
BsnI GGCC 4 cut(s) 35, 561, 758, 889
Bso31I GGTCTC 2 cut(s) 340, 866
Bsp1286I GDGCHC 4 cut(s) 155, 304, 646, 798
Bsp143I GATC 5 cut(s) 21, 834, 838, 897, 979
Bsp19I CCATGG 2 cut(s) 175, 646
BspACI CCGC 3 cut(s) 73, 562, 933
BspANI GGCC 4 cut(s) 35, 561, 758, 889
BspCNI CTCAG 3 cut(s) 190, 304, 569
BspDI ATCGAT 1 cut(s) 837
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 6 cut(s) 331, 405, 643, 675, 700, 795
BspMI ACCTGC 3 cut(s) 341, 584, 757
BspPI GGATC 2 cut(s) 833, 974
BspT107I GGYRCC 2 cut(s) 329, 698
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 2 cut(s) 340, 866
BsrI ACTGG 2 cut(s) 407, 832
BssMI GATC 5 cut(s) 21, 834, 838, 897, 979
BssNI GRCGYC 1 cut(s) 699
BssT1I CCWWGG 5 cut(s) 30, 175, 338, 646, 681
Bst2UI CCWGG 6 cut(s) 131, 167, 221, 891, 911, 994
Bst4CI ACNGT 3 cut(s) 373, 424, 454
BstACI GRCGYC 1 cut(s) 699
BstAFI CTTAAG 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 717, 747, 751
BstDEI CTNAG 4 cut(s) 198, 272, 312, 556
BstDSI CCRYGG 3 cut(s) 175, 372, 646
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 1 cut(s) 641
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 2 cut(s) 77, 701
BstKTI GATC 5 cut(s) 24, 837, 841, 900, 982
BstMAI GTCTC 4 cut(s) 311, 340, 443, 866
BstMBI GATC 5 cut(s) 21, 834, 838, 897, 979
BstMWI GCNNNNNNNGC 1 cut(s) 755
BstNI CCWGG 6 cut(s) 131, 167, 221, 891, 911, 994
BstNSI RCATGY 2 cut(s) 232, 494
BstPAI GACNNNNGTC 2 cut(s) 222, 365
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 7 cut(s) 129, 165, 219, 510, 889, 909, 992
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 460, 706, 940
BstV2I GAAGAC 2 cut(s) 539, 953
Bsu15I ATCGAT 1 cut(s) 837
Bsu36I CCTNAGG 2 cut(s) 198, 556
BsuRI GGCC 4 cut(s) 35, 561, 758, 889
BsuTUI ATCGAT 1 cut(s) 837
BtgI CCRYGG 3 cut(s) 175, 372, 646
BtsCI GGATG 1 cut(s) 641
BtsIMutI CAGTG 3 cut(s) 243, 309, 420
BveI ACCTGC 3 cut(s) 341, 584, 757
Cac8I GCNNGC 3 cut(s) 717, 747, 751
CfoI GCGC 2 cut(s) 77, 701
Cfr13I GGNCC 5 cut(s) 127, 578, 673, 756, 913
ClaI ATCGAT 1 cut(s) 837
CseI GACGC 1 cut(s) 615
Csp6I GTAC 3 cut(s) 330, 379, 520
CspCI CAANNNNNGTGG 4 cut(s) 277, 312, 907, 942
CviAII CATG 9 cut(s) 7, 176, 229, 278, 443, 491, 584, 647, 895
CviQI GTAC 3 cut(s) 330, 379, 520
DdeI CTNAG 4 cut(s) 198, 272, 312, 556
DinI GGCGCC 1 cut(s) 700
DpnI GATC 5 cut(s) 23, 836, 840, 899, 981
DpnII GATC 5 cut(s) 21, 834, 838, 897, 979
EciI GGCGGA 1 cut(s) 948
Eco130I CCWWGG 5 cut(s) 30, 175, 338, 646, 681
Eco147I AGGCCT 1 cut(s) 889
Eco24I GRGCYC 3 cut(s) 155, 646, 798
Eco31I GGTCTC 2 cut(s) 340, 866
Eco47I GGWCC 4 cut(s) 127, 578, 673, 913
Eco57I CTGAAG 1 cut(s) 868
Eco81I CCTNAGG 2 cut(s) 198, 556
Eco91I GGTNACC 1 cut(s) 215
EcoO109I RGGNCCY 2 cut(s) 127, 673
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 6 cut(s) 129, 165, 219, 889, 909, 992
EcoT14I CCWWGG 5 cut(s) 30, 175, 338, 646, 681
EcoT38I GRGCYC 3 cut(s) 155, 646, 798
EgeI GGCGCC 1 cut(s) 700
EheI GGCGCC 1 cut(s) 700
ErhI CCWWGG 5 cut(s) 30, 175, 338, 646, 681
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 9 cut(s) 10, 179, 232, 281, 446, 494, 587, 650, 898
FalI AAGNNNNNCTT 4 cut(s) 524, 556, 860, 892
FaqI GGGAC 2 cut(s) 494, 989
FatI CATG 9 cut(s) 6, 175, 228, 277, 442, 490, 583, 646, 894
FauNDI CATATG 1 cut(s) 820
FbaI TGATCA 1 cut(s) 897
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 5 cut(s) 73, 474, 562, 720, 954
FokI GGATG 1 cut(s) 648
FriOI GRGCYC 3 cut(s) 155, 646, 798
Fsp4HI GCNGC 5 cut(s) 73, 474, 562, 720, 954
GlaI GCGC 2 cut(s) 76, 700
GluI GCNGC 5 cut(s) 73, 474, 562, 720, 954
GsaI CCCAGC 1 cut(s) 442
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 4 cut(s) 35, 561, 758, 889
HapII CCGG 3 cut(s) 512, 603, 791
HgaI GACGC 1 cut(s) 615
HhaI GCGC 2 cut(s) 77, 701
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 9 cut(s) 10, 179, 232, 281, 446, 494, 587, 650, 898
Hin6I GCGC 2 cut(s) 75, 699
HinP1I GCGC 2 cut(s) 75, 699
HincII GTYRAC 3 cut(s) 43, 688, 814
HindII GTYRAC 3 cut(s) 43, 688, 814
HindIII AAGCTT 1 cut(s) 293
HpaII CCGG 3 cut(s) 512, 603, 791
HphI GGTGA 4 cut(s) 227, 361, 599, 982
Hpy166II GTNNAC 4 cut(s) 43, 418, 688, 814
Hpy188I TCNGA 2 cut(s) 17, 94
Hpy188III TCNNGA 7 cut(s) 107, 666, 842, 919, 943, 983, 1035
Hpy8I GTNNAC 4 cut(s) 43, 418, 688, 814
HpyAV CCTTC 4 cut(s) 862, 886, 930, 966
HpyCH4III ACNGT 3 cut(s) 373, 424, 454
HpyCH4V TGCA 3 cut(s) 123, 593, 766
HpyF10VI GCNNNNNNNGC 1 cut(s) 755
HpyF3I CTNAG 4 cut(s) 198, 272, 312, 556
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 9 cut(s) 10, 179, 232, 281, 446, 494, 587, 650, 898
HspAI GCGC 2 cut(s) 75, 699
KasI GGCGCC 1 cut(s) 698
KpnI GGTACC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 897
Kzo9I GATC 5 cut(s) 21, 834, 838, 897, 979
LmnI GCTCC 4 cut(s) 395, 487, 641, 793
Lsp1109I GCAGC 3 cut(s) 460, 706, 940
LweI GCATC 3 cut(s) 100, 255, 580
MaeIII GTNAC 6 cut(s) 62, 179, 215, 224, 367, 454
MalI GATC 5 cut(s) 23, 836, 840, 899, 981
MboI GATC 5 cut(s) 21, 834, 838, 897, 979
MboII GAAGA 5 cut(s) 64, 334, 532, 544, 958
MhlI GDGCHC 4 cut(s) 155, 304, 646, 798
MluCI AATT 2 cut(s) 260, 861
Mly113I GGCGCC 1 cut(s) 699
MseI TTAA 5 cut(s) 135, 483, 498, 549, 858
MslI CAYNNNNRTG 2 cut(s) 588, 785
MspA1I CMGCKG 1 cut(s) 953
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 3 cut(s) 512, 603, 791
MspR9I CCNGG 7 cut(s) 131, 167, 221, 512, 891, 911, 994
MvaI CCWGG 6 cut(s) 131, 167, 221, 891, 911, 994
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 1 cut(s) 755
NarI GGCGCC 1 cut(s) 699
NciI CCSGG 1 cut(s) 512
NcoI CCATGG 2 cut(s) 175, 646
NdeI CATATG 1 cut(s) 820
NdeII GATC 5 cut(s) 21, 834, 838, 897, 979
NlaIII CATG 9 cut(s) 10, 179, 232, 281, 446, 494, 587, 650, 898
NlaIV GGNNCC 6 cut(s) 331, 405, 643, 675, 700, 795
NmuCI GTSAC 6 cut(s) 62, 179, 215, 224, 367, 454
NspI RCATGY 2 cut(s) 232, 494
PaqCI CACCTGC 1 cut(s) 757
PasI CCCWGGG 1 cut(s) 166
PceI AGGCCT 1 cut(s) 889
PciI ACATGT 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 510
PkrI GCNGC 5 cut(s) 74, 475, 563, 721, 955
PluTI GGCGCC 1 cut(s) 702
PpuMI RGGWCCY 2 cut(s) 127, 673
PscI ACATGT 1 cut(s) 228
PshAI GACNNNNGTC 2 cut(s) 222, 365
Psp5II RGGWCCY 2 cut(s) 127, 673
Psp6I CCWGG 6 cut(s) 129, 165, 219, 889, 909, 992
PspEI GGTNACC 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 6 cut(s) 129, 165, 219, 889, 909, 992
PspN4I GGNNCC 6 cut(s) 331, 405, 643, 675, 700, 795
PspPI GGNCC 5 cut(s) 127, 578, 673, 756, 913
PspPPI RGGWCCY 2 cut(s) 127, 673
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 3 cut(s) 331, 380, 521
RsaNI GTAC 3 cut(s) 330, 379, 520
RseI CAYNNNNRTG 2 cut(s) 588, 785
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 5 cut(s) 135, 483, 498, 549, 858
SatI GCNGC 5 cut(s) 73, 474, 562, 720, 954
Sau3AI GATC 5 cut(s) 21, 834, 838, 897, 979
Sau96I GGNCC 5 cut(s) 127, 578, 673, 756, 913
ScaI AGTACT 1 cut(s) 380
ScrFI CCNGG 7 cut(s) 131, 167, 221, 512, 891, 911, 994
SduI GDGCHC 4 cut(s) 155, 304, 646, 798
SfaNI GCATC 3 cut(s) 100, 255, 580
SfoI GGCGCC 1 cut(s) 700
SinI GGWCC 4 cut(s) 127, 578, 673, 913
SmiMI CAYNNNNRTG 2 cut(s) 588, 785
SmlI CTYRAG 3 cut(s) 482, 917, 1013
SmoI CTYRAG 3 cut(s) 482, 917, 1013
Sse9I AATT 2 cut(s) 260, 861
SseBI AGGCCT 1 cut(s) 889
SsiI CCGC 3 cut(s) 73, 562, 933
SspDI GGCGCC 1 cut(s) 698
SspI AATATT 1 cut(s) 139
StuI AGGCCT 1 cut(s) 889
StyD4I CCNGG 7 cut(s) 129, 165, 219, 510, 889, 909, 992
StyI CCWWGG 5 cut(s) 30, 175, 338, 646, 681
TaaI ACNGT 3 cut(s) 373, 424, 454
TaqI TCGA 3 cut(s) 687, 837, 879
TasI AATT 2 cut(s) 260, 861
TatI WGTACW 1 cut(s) 378
TauI GCSGC 2 cut(s) 75, 564
Tru1I TTAA 5 cut(s) 135, 483, 498, 549, 858
Tru9I TTAA 5 cut(s) 135, 483, 498, 549, 858
TscAI CASTG 3 cut(s) 250, 316, 427
TseFI GTSAC 6 cut(s) 62, 179, 215, 224, 367, 454
TseI GCWGC 3 cut(s) 473, 719, 953
Tsp45I GTSAC 6 cut(s) 62, 179, 215, 224, 367, 454
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 2 cut(s) 50, 401
TspRI CASTG 3 cut(s) 250, 316, 427
Vha464I CTTAAG 1 cut(s) 482
VpaK11BI GGWCC 4 cut(s) 127, 578, 673, 913
XceI RCATGY 2 cut(s) 232, 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 173, 990
XmiI GTMKAC 1 cut(s) 687
ZrmI AGTACT 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.