RchiOBHm_Chr3g0486231

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002178
Physical Location & Seq
Reverse (-)
33138301 .. 33140828
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45075

Sequence Viewer

Length: 1131 bp
ATGGAGACTTTCCTATTCACATCTGAATCTGTGAACGAGGGACACCCCGACAAGCTGTGCGACCAGATCTCTGACGCAGTGCTTGATGCTTGCCTTGAGCAGGATCCTGACAGCAAGGTTGCCTGTGAGACATGTACCAAAACAAACATGGTAATGGTGTTCGGAGAGATCACCACCAAGGCCACTGTTGACTACGAGAAGATTGTCCGTGACACATGCCGCAGCATTGGGTTTGTTTCTGATGATGTCGGTCTTGATGCTGATAACTGCAAGGTCCTAGTCAACATTGAGCAGCAGAGCCCAGATATTGCCCAGGGTGGTCACATGTTTGGCTATGCCACTGATGAAACCCCTGAGTTGATGCCCCTTAGCCATGTCCTTGCAACCAAGCTTGGTGCTCGCCTCACTGAAGTTAGAAAGAATGGTACTTGCGCATGGCTGAGACCTGATGGCAAGACCCAGGTGACAGTTGAATACCAGAATGAAGCCGGTGCTATGGTTCCACTACGTGTCCACACTGTTCTTATCTCCACCCAGCATGACGAGACTGTCACAAATGATGAGATTGCTGCTGACCTCAAGGAGCATGTTATTAAGCCTGTCATCCCCGAGAAGTACCTGGATGAGAAGACAATCTTCCATCTTAACCCCTCTGGTCGCTTTGTGATTGGTGGTCCTCATGGTGATGCAGGTCTCACCGGACGCAAGATCATTATTGATACCTATGGTGGTTGGGGAGCCCATGGTGGTGGTGCTTTCTCTGGAAAGGACCCTACCAAGGTGGACAGGAGTGGTGCTTACATTGTCAGGCAGGCTGCCAAGAGCATTGTAGCAAATGGTCTTGCTCGCAGGGCCATCGTGCAGGTCTCCTATGCCATTGGTGTCCCTGAGCCCTTGTCGGTCTTCGTCGACACATACGGAACTGGAAAGATCCCTGACAAGGAGATCCTTAAAATTGTGAAGGAGAACTTTGACTTCAGGCCGGGTATGATGACCATCAACCTGGACCTCAAGAGGGGTGGCAATGGAAGATTCTTGAAGACAGCCGCCTATGGGCACTTCGGAAGGGATGACCCTGACTTCACCTGGGAGGTTGTGAAGCCCCTCAAGTGGGAGAAGCCTCAGTCTTGA

Protein Analysis

376

Amino Acids

41.19

Weight (kDa)

5.44

Isoelectric Point (pI)

22.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 2.5e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 107 - 221 7.6e-43 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 223 - 364 3.9e-60 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
Acc16I TGCGCA 1 cut(s) 433
Acc36I ACCTGC 2 cut(s) 680, 853
AccI GTMKAC 1 cut(s) 909
AciI CCGC 2 cut(s) 220, 1047
AclWI GGATC 4 cut(s) 98, 111, 925, 940
AcuI CTGAAG 2 cut(s) 429, 961
AdeI CACNNNGTG 1 cut(s) 509
AfaI GTAC 3 cut(s) 136, 427, 617
AfiI CCNNNNNNNGG 7 cut(s) 100, 778, 940, 1015, 1053, 1110, 1111
AflIII ACRYGT 3 cut(s) 131, 324, 508
AgsI TTSAA 2 cut(s) 473, 1039
AjnI CCWGG 5 cut(s) 312, 459, 618, 1002, 1085
AluBI AGCT 2 cut(s) 55, 391
AluI AGCT 2 cut(s) 55, 391
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 5 cut(s) 122, 436, 539, 698, 871
AlwI GGATC 4 cut(s) 98, 111, 925, 940
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 3 cut(s) 180, 852, 980
ApeKI GCWGC 4 cut(s) 222, 292, 569, 815
AspLEI GCGC 1 cut(s) 434
AspS9I GGNCC 5 cut(s) 274, 674, 769, 852, 1006
AsuC2I CCSGG 1 cut(s) 984
AsuHPI GGTGA 5 cut(s) 163, 475, 688, 695, 1075
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 4 cut(s) 274, 674, 769, 1006
BaeGI GKGCMC 1 cut(s) 1059
BamHI GGATCC 1 cut(s) 103
BanII GRGCYC 3 cut(s) 302, 742, 894
BbsI GAAGAC 3 cut(s) 635, 895, 1046
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 4 cut(s) 234, 304, 556, 802
BccI CCATC 4 cut(s) 443, 648, 863, 1004
BcgI CGANNNNNNTGC 2 cut(s) 838, 872
BciT130I CCWGG 5 cut(s) 314, 461, 620, 1004, 1087
BcnI CCSGG 1 cut(s) 984
BcoDI GTCTC 5 cut(s) 122, 436, 539, 698, 871
BfaI CTAG 1 cut(s) 278
BfuAI ACCTGC 2 cut(s) 680, 853
BglII AGATCT 1 cut(s) 66
BisI GCNGC 6 cut(s) 220, 223, 293, 570, 816, 1047
BlsI GCNGC 6 cut(s) 221, 224, 294, 571, 817, 1048
Bme1390I CCNGG 6 cut(s) 314, 461, 620, 984, 1004, 1087
Bme18I GGWCC 4 cut(s) 274, 674, 769, 1006
BmeT110I CYCGRG 1 cut(s) 608
BmgT120I GGNCC 5 cut(s) 274, 674, 769, 852, 1006
BmiI GGNNCC 4 cut(s) 105, 501, 739, 771
BmrFI CCNGG 6 cut(s) 314, 461, 620, 984, 1004, 1087
BmsI GCATC 4 cut(s) 76, 247, 351, 676
BpiI GAAGAC 3 cut(s) 635, 895, 1046
Bpu10I CCTNAGC 2 cut(s) 368, 888
BpuEI CTTGAG 4 cut(s) 116, 563, 995, 1091
BpuMI CCSGG 1 cut(s) 984
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 995
BsaI GGTCTC 3 cut(s) 436, 698, 871
BsaJI CCNNGG 7 cut(s) 177, 312, 313, 459, 742, 777, 1086
BsaWI WCCGGW 1 cut(s) 698
Bsc4I CCNNNNNNNGG 7 cut(s) 100, 778, 940, 1015, 1053, 1110, 1111
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 1 cut(s) 928
Bse3DI GCAATG 1 cut(s) 1030
Bse8I GATNNNNATC 1 cut(s) 995
BseBI CCWGG 5 cut(s) 314, 461, 620, 1004, 1087
BseDI CCNNGG 7 cut(s) 177, 312, 313, 459, 742, 777, 1086
BseGI GGATG 3 cut(s) 603, 628, 1075
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 7 cut(s) 100, 778, 940, 1015, 1053, 1110, 1111
BseMI GCAATG 1 cut(s) 1030
BseMII CTCAG 3 cut(s) 345, 431, 879
BseNI ACTGG 1 cut(s) 928
BseSI GKGCMC 1 cut(s) 1059
BseXI GCAGC 4 cut(s) 234, 304, 556, 802
BseYI CCCAGC 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 881
BshFI GGCC 3 cut(s) 182, 854, 982
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 3 cut(s) 489, 699, 983
BslFI GGGAC 2 cut(s) 54, 869
BslI CCNNNNNNNGG 7 cut(s) 100, 778, 940, 1015, 1053, 1110, 1111
BsmAI GTCTC 5 cut(s) 122, 436, 539, 698, 871
BsmFI GGGAC 2 cut(s) 54, 869
BsnI GGCC 3 cut(s) 182, 854, 982
Bso31I GGTCTC 3 cut(s) 436, 698, 871
BsoBI CYCGRG 1 cut(s) 608
Bsp1286I GDGCHC 5 cut(s) 302, 400, 742, 894, 1059
Bsp143I GATC 6 cut(s) 66, 103, 168, 708, 930, 945
Bsp19I CCATGG 1 cut(s) 742
BspACI CCGC 2 cut(s) 220, 1047
BspANI GGCC 3 cut(s) 182, 854, 982
BspCNI CTCAG 3 cut(s) 346, 432, 880
BspLI GGNNCC 4 cut(s) 105, 501, 739, 771
BspMI ACCTGC 2 cut(s) 680, 853
BspPI GGATC 4 cut(s) 98, 111, 925, 940
BspTNI GGTCTC 3 cut(s) 436, 698, 871
BsrDI GCAATG 1 cut(s) 1030
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 1 cut(s) 928
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 7 cut(s) 177, 312, 313, 459, 742, 777, 1086
BssMI GATC 6 cut(s) 66, 103, 168, 708, 930, 945
BssT1I CCWWGG 3 cut(s) 177, 742, 777
Bst2UI CCWGG 5 cut(s) 314, 461, 620, 1004, 1087
Bst4CI ACNGT 4 cut(s) 187, 469, 520, 550
BstBAI YACGTR 1 cut(s) 509
BstC8I GCNNGC 4 cut(s) 91, 400, 813, 847
BstDEI CTNAG 5 cut(s) 354, 368, 440, 888, 1122
BstDSI CCRYGG 1 cut(s) 742
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 3 cut(s) 603, 628, 1075
BstHHI GCGC 1 cut(s) 434
BstKTI GATC 6 cut(s) 69, 106, 171, 711, 933, 948
BstMAI GTCTC 5 cut(s) 122, 436, 539, 698, 871
BstMBI GATC 6 cut(s) 66, 103, 168, 708, 930, 945
BstMWI GCNNNNNNNGC 1 cut(s) 851
BstNI CCWGG 5 cut(s) 314, 461, 620, 1004, 1087
BstNSI RCATGY 4 cut(s) 135, 219, 328, 590
BstSCI CCNGG 6 cut(s) 312, 459, 618, 982, 1002, 1085
BstSLI GKGCMC 1 cut(s) 1059
BstV1I GCAGC 4 cut(s) 234, 304, 556, 802
BstV2I GAAGAC 3 cut(s) 635, 895, 1046
BstX2I RGATCY 4 cut(s) 66, 103, 930, 945
BstXI CCANNNNNNTGG 2 cut(s) 749, 1003
BstYI RGATCY 4 cut(s) 66, 103, 930, 945
BsuRI GGCC 3 cut(s) 182, 854, 982
BtgI CCRYGG 1 cut(s) 742
BtsCI GGATG 3 cut(s) 603, 628, 1075
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 5 cut(s) 84, 183, 339, 405, 516
BveI ACCTGC 2 cut(s) 680, 853
Cac8I GCNNGC 4 cut(s) 91, 400, 813, 847
CfoI GCGC 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 5 cut(s) 274, 674, 769, 852, 1006
CseI GACGC 2 cut(s) 83, 711
Csp6I GTAC 3 cut(s) 135, 426, 616
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviQI GTAC 3 cut(s) 135, 426, 616
DdeI CTNAG 5 cut(s) 354, 368, 440, 888, 1122
DpnI GATC 6 cut(s) 68, 105, 170, 710, 932, 947
DpnII GATC 6 cut(s) 66, 103, 168, 708, 930, 945
DraIII CACNNNGTG 1 cut(s) 509
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 177, 742, 777
Eco24I GRGCYC 3 cut(s) 302, 742, 894
Eco31I GGTCTC 3 cut(s) 436, 698, 871
Eco47I GGWCC 4 cut(s) 274, 674, 769, 1006
Eco57I CTGAAG 2 cut(s) 429, 961
Eco88I CYCGRG 1 cut(s) 608
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO109I RGGNCCY 2 cut(s) 274, 769
EcoRII CCWGG 5 cut(s) 312, 459, 618, 1002, 1085
EcoT14I CCWWGG 3 cut(s) 177, 742, 777
EcoT38I GRGCYC 3 cut(s) 302, 742, 894
ErhI CCWWGG 3 cut(s) 177, 742, 777
FalI AAGNNNNNCTT 4 cut(s) 620, 652, 953, 985
FaqI GGGAC 2 cut(s) 54, 869
FblI GTMKAC 1 cut(s) 909
Fnu4HI GCNGC 6 cut(s) 220, 223, 293, 570, 816, 1047
FokI GGATG 3 cut(s) 590, 635, 1082
FriOI GRGCYC 3 cut(s) 302, 742, 894
Fsp4HI GCNGC 6 cut(s) 220, 223, 293, 570, 816, 1047
FspBI CTAG 1 cut(s) 278
FspI TGCGCA 1 cut(s) 433
GlaI GCGC 1 cut(s) 433
GluI GCNGC 6 cut(s) 220, 223, 293, 570, 816, 1047
GsaI CCCAGC 1 cut(s) 538
HaeIII GGCC 3 cut(s) 182, 854, 982
HapII CCGG 3 cut(s) 489, 699, 983
HgaI GACGC 2 cut(s) 83, 711
HhaI GCGC 1 cut(s) 434
Hin6I GCGC 1 cut(s) 432
HinP1I GCGC 1 cut(s) 432
HincII GTYRAC 3 cut(s) 190, 283, 910
HindII GTYRAC 3 cut(s) 190, 283, 910
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 2 cut(s) 26, 1032
HpaII CCGG 3 cut(s) 489, 699, 983
HphI GGTGA 5 cut(s) 163, 475, 688, 695, 1075
Hpy166II GTNNAC 6 cut(s) 34, 190, 283, 514, 784, 910
Hpy188I TCNGA 5 cut(s) 25, 73, 164, 241, 1064
Hpy188III TCNNGA 6 cut(s) 107, 254, 762, 1012, 1036, 1128
Hpy8I GTNNAC 6 cut(s) 34, 190, 283, 514, 784, 910
Hpy99I CGWCG 1 cut(s) 911
HpyAV CCTTC 2 cut(s) 955, 1059
HpyCH4III ACNGT 4 cut(s) 187, 469, 520, 550
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 4 cut(s) 270, 383, 689, 862
HpyF10VI GCNNNNNNNGC 1 cut(s) 851
HpyF3I CTNAG 5 cut(s) 354, 368, 440, 888, 1122
HpySE526I ACGT 1 cut(s) 508
HspAI GCGC 1 cut(s) 432
Kzo9I GATC 6 cut(s) 66, 103, 168, 708, 930, 945
LmnI GCTCC 2 cut(s) 583, 737
Lsp1109I GCAGC 4 cut(s) 234, 304, 556, 802
LweI GCATC 4 cut(s) 76, 247, 351, 676
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 508
MaeIII GTNAC 4 cut(s) 209, 320, 463, 550
MalI GATC 6 cut(s) 68, 105, 170, 710, 932, 947
MboI GATC 6 cut(s) 66, 103, 168, 708, 930, 945
MboII GAAGA 6 cut(s) 211, 628, 640, 895, 1041, 1051
MflI RGATCY 4 cut(s) 66, 103, 930, 945
MhlI GDGCHC 5 cut(s) 302, 400, 742, 894, 1059
MluCI AATT 1 cut(s) 954
MseI TTAA 3 cut(s) 594, 645, 951
MslI CAYNNNNRTG 3 cut(s) 152, 684, 747
MspI CCGG 3 cut(s) 489, 699, 983
MspR9I CCNGG 6 cut(s) 314, 461, 620, 984, 1004, 1087
MvaI CCWGG 5 cut(s) 314, 461, 620, 1004, 1087
MwoI GCNNNNNNNGC 1 cut(s) 851
NciI CCSGG 1 cut(s) 984
NcoI CCATGG 1 cut(s) 742
NdeII GATC 6 cut(s) 66, 103, 168, 708, 930, 945
NlaIV GGNNCC 4 cut(s) 105, 501, 739, 771
NmuCI GTSAC 4 cut(s) 209, 320, 463, 550
NsbI TGCGCA 1 cut(s) 433
NspI RCATGY 4 cut(s) 135, 219, 328, 590
PasI CCCWGGG 1 cut(s) 313
PciI ACATGT 2 cut(s) 131, 324
PfeI GAWTC 2 cut(s) 26, 1032
PkrI GCNGC 6 cut(s) 221, 224, 294, 571, 817, 1048
Ppu21I YACGTR 1 cut(s) 509
PpuMI RGGWCCY 2 cut(s) 274, 769
PscI ACATGT 2 cut(s) 131, 324
Psp5II RGGWCCY 2 cut(s) 274, 769
Psp6I CCWGG 5 cut(s) 312, 459, 618, 1002, 1085
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 5 cut(s) 312, 459, 618, 1002, 1085
PspN4I GGNNCC 4 cut(s) 105, 501, 739, 771
PspPI GGNCC 5 cut(s) 274, 674, 769, 852, 1006
PspPPI RGGWCCY 2 cut(s) 274, 769
PsuI RGATCY 4 cut(s) 66, 103, 930, 945
RsaI GTAC 3 cut(s) 136, 427, 617
RsaNI GTAC 3 cut(s) 135, 426, 616
RseI CAYNNNNRTG 3 cut(s) 152, 684, 747
SalI GTCGAC 1 cut(s) 908
SaqAI TTAA 3 cut(s) 594, 645, 951
SatI GCNGC 6 cut(s) 220, 223, 293, 570, 816, 1047
Sau3AI GATC 6 cut(s) 66, 103, 168, 708, 930, 945
Sau96I GGNCC 5 cut(s) 274, 674, 769, 852, 1006
ScrFI CCNGG 6 cut(s) 314, 461, 620, 984, 1004, 1087
SduI GDGCHC 5 cut(s) 302, 400, 742, 894, 1059
SfaNI GCATC 4 cut(s) 76, 247, 351, 676
SinI GGWCC 4 cut(s) 274, 674, 769, 1006
SmiMI CAYNNNNRTG 3 cut(s) 152, 684, 747
SmlI CTYRAG 4 cut(s) 95, 578, 1010, 1106
SmoI CTYRAG 4 cut(s) 95, 578, 1010, 1106
Sse9I AATT 1 cut(s) 954
SsiI CCGC 2 cut(s) 220, 1047
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 6 cut(s) 312, 459, 618, 982, 1002, 1085
StyI CCWWGG 3 cut(s) 177, 742, 777
TaaI ACNGT 4 cut(s) 187, 469, 520, 550
TaiI ACGT 1 cut(s) 511
TaqI TCGA 1 cut(s) 909
TaqII GACCGA 2 cut(s) 239, 889
TasI AATT 1 cut(s) 954
TauI GCSGC 2 cut(s) 222, 1049
TfiI GAWTC 2 cut(s) 26, 1032
Tru1I TTAA 3 cut(s) 594, 645, 951
Tru9I TTAA 3 cut(s) 594, 645, 951
TscAI CASTG 5 cut(s) 84, 190, 346, 412, 523
TseFI GTSAC 4 cut(s) 209, 320, 463, 550
TseI GCWGC 4 cut(s) 222, 292, 569, 815
Tsp45I GTSAC 4 cut(s) 209, 320, 463, 550
TspDTI ATGAA 2 cut(s) 360, 498
TspGWI ACGGA 2 cut(s) 197, 933
TspRI CASTG 5 cut(s) 84, 190, 346, 412, 523
VpaK11BI GGWCC 4 cut(s) 274, 674, 769, 1006
XagI CCTNNNNNAGG 1 cut(s) 98
XceI RCATGY 4 cut(s) 135, 219, 328, 590
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 909
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.