MD04G1187700.v1.1

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002178
Physical Location & Seq
Reverse (-)
27825911 .. 27828063
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1187700.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGCTTGTAAGAATGGCAGAGCCAGCAGACACCTTCCTGTTTACTTCCGAGTCTGTGAACGAGGGGCACCCTGATAAGCTCTGTGATCAGATTTCTGATGCAGTGCTAGATGCATGCCTCGAGCAAGATCCCGACAGCAAAGTTGCCTGTGAGTCTTGCACCAAGACGAACATGGTCATGATCTTCGGAGAGATTACAACCAAAGCCAACTTGGATTACGAAAAGATTGTGCGCGACACCTGCCGTACAATTGGATATGTTTCCGACAGTGTTGGTCTTGATGCTGACAACTGCAAGGTCTTGGTTAACATTGAGCAACAGAGCCCTGATATCGCCCAGGGTGTCCACGGTCACCTGACCAAGCGGCCGGAGGAAATTGGCGCAGGTGACCAGGGTCACATGTTTGGTTATGCCACTGATGAGACCACTGAGCTTATGCCTCTGAGCCATGTTCTTGCTACCAACCTTGGTGCCCGTCTCACTGAAGTTCGCAAGAATGGAACCTTGTCGTGGTTGAGACCTGATGGAAAAACACAAGTCACTGTTGAGTACTACAATGACCATGGCGCTATGGTTCCAATCCGCATCCACACGGTTCTCATTTCTACTCAGCATGACGAAACTGTTACCAATGATCAGATTGCTGCTGACCTCAAAGAGCATGTTATCAAGCCTGTTGTTCCTGAGAAGTACCTAGACGACAAGACTATTTTCCATCTTAACCCATCAGGTCGGTTTGTCATTGGTGGACCTCACGGTGATGCTGGCCTCACTGGCCGCAAAATCATCGTTGACACCTACGGTGGCTGGGGAGCCCATGGTGGTGGTGCCTTCTCCGGCAAGGACCCCACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGACAGGCTGCTAAGAGCATTGTGGCCAACGGTCTTGCTCGCAGGTGCATAGTGCAGGTCTCGTATGCCATTGGTGTTCCCGAGCCACTATCAGTGTTCGTTGACAGTTATGGCACTGGAAAGATTCCTGACAAGGAGATTCTTAAACTAGTAAAGGAGAGCTTCGATTTCAGGCCGGGAATGATTGCGATTAACCTTGATCTCAAGCGGGGCAGGTATTTGAAGACGGCTGCTTATGGACACTTCGGTAGGGATGACCCTGACTTCTCATGGGAAGTGGTGAAGCCGTTGAAGTGGGAAAAGCCCCAAGGGTAG

Protein Analysis

399

Amino Acids

43.66

Weight (kDa)

5.64

Isoelectric Point (pI)

25.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 12 - 109 6.6e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 125 - 246 5.6e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 248 - 386 1.1e-62 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 248, 374, 915
Acc36I ACCTGC 5 cut(s) 248, 374, 915, 928, 1086
AccB1I GGYRCC 4 cut(s) 66, 470, 827, 869
AccI GTMKAC 1 cut(s) 858
AccII CGCG 1 cut(s) 234
AciI CCGC 4 cut(s) 364, 583, 778, 1090
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 3 cut(s) 365, 775, 906
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 3 cut(s) 247, 551, 692
AfiI CCNNNNNNNGG 2 cut(s) 510, 853
AflIII ACRYGT 1 cut(s) 399
AgsI TTSAA 2 cut(s) 1105, 1174
AhlI ACTAGT 1 cut(s) 1030
AjnI CCWGG 2 cut(s) 336, 390
AluBI AGCT 3 cut(s) 79, 433, 1044
AluI AGCT 3 cut(s) 79, 433, 1044
Alw26I GTCTC 4 cut(s) 416, 482, 511, 946
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 2 cut(s) 119, 962
AoxI GGCC 5 cut(s) 365, 766, 775, 906, 1055
ApeKI GCWGC 3 cut(s) 644, 890, 1112
AspLEI GCGC 3 cut(s) 234, 383, 569
AspS9I GGNCC 2 cut(s) 749, 844
AsuC2I CCSGG 1 cut(s) 1059
AsuHPI GGTGA 4 cut(s) 344, 398, 770, 1174
AvaI CYCGRG 2 cut(s) 119, 962
AvaII GGWCC 2 cut(s) 749, 844
BaeGI GKGCMC 2 cut(s) 69, 475
BalI TGGCCA 1 cut(s) 908
BanI GGYRCC 4 cut(s) 66, 470, 827, 869
BanII GRGCYC 2 cut(s) 326, 817
BbsI GAAGAC 1 cut(s) 1112
BbvI GCAGC 3 cut(s) 631, 877, 1099
BccI CCATC 3 cut(s) 518, 723, 733
BceAI ACGGC 3 cut(s) 228, 1125, 1153
BcgI CGANNNNNNTGC 2 cut(s) 769, 803
BciT130I CCWGG 2 cut(s) 338, 392
BclI TGATCA 2 cut(s) 85, 634
BcnI CCSGG 1 cut(s) 1059
BcoDI GTCTC 4 cut(s) 416, 482, 511, 946
BcuI ACTAGT 1 cut(s) 1030
BfaI CTAG 3 cut(s) 107, 695, 1031
BfoI RGCGCY 1 cut(s) 570
BfuAI ACCTGC 5 cut(s) 248, 374, 915, 928, 1086
BglI GCCNNNNNGGC 1 cut(s) 774
BisI GCNGC 5 cut(s) 365, 645, 778, 891, 1113
BlsI GCNGC 5 cut(s) 366, 646, 779, 892, 1114
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 3 cut(s) 338, 392, 1059
Bme18I GGWCC 2 cut(s) 749, 844
BmeT110I CYCGRG 2 cut(s) 119, 962
BmgT120I GGNCC 2 cut(s) 749, 844
BmiI GGNNCC 8 cut(s) 68, 472, 502, 576, 814, 829, 846, 871
BmrFI CCNGG 3 cut(s) 338, 392, 1059
BmsI GCATC 5 cut(s) 88, 100, 271, 594, 751
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 1112
BpuEI CTTGAG 1 cut(s) 1070
BpuMI CCSGG 1 cut(s) 1059
BsaI GGTCTC 3 cut(s) 416, 511, 946
BsaJI CCNNGG 9 cut(s) 336, 337, 346, 391, 466, 562, 817, 852, 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 853
Bse1I ACTGG 2 cut(s) 778, 1003
BseBI CCWGG 2 cut(s) 338, 392
BseDI CCNNGG 9 cut(s) 336, 337, 346, 391, 466, 562, 817, 852, 1189
BseGI GGATG 2 cut(s) 585, 1141
BseLI CCNNNNNNNGG 2 cut(s) 510, 853
BseMII CTCAG 4 cut(s) 420, 434, 623, 675
BseNI ACTGG 2 cut(s) 778, 1003
BseSI GKGCMC 2 cut(s) 69, 475
BseX3I CGGCCG 1 cut(s) 365
BseXI GCAGC 3 cut(s) 631, 877, 1099
BseYI CCCAGC 1 cut(s) 807
BsgI GTGCAG 1 cut(s) 956
Bsh1236I CGCG 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 368
BshFI GGCC 5 cut(s) 367, 768, 777, 908, 1057
BshNI GGYRCC 4 cut(s) 66, 470, 827, 869
BsiEI CGRYCG 1 cut(s) 368
BsiHKCI CYCGRG 2 cut(s) 119, 962
BsiSI CCGG 3 cut(s) 368, 837, 1058
BslI CCNNNNNNNGG 2 cut(s) 510, 853
BsmAI GTCTC 4 cut(s) 416, 482, 511, 946
BsmBI CGTCTC 1 cut(s) 482
BsnI GGCC 5 cut(s) 367, 768, 777, 908, 1057
Bso31I GGTCTC 3 cut(s) 416, 511, 946
BsoBI CYCGRG 2 cut(s) 119, 962
Bsp1286I GDGCHC 4 cut(s) 69, 326, 475, 817
Bsp143I GATC 5 cut(s) 85, 127, 180, 634, 1081
Bsp19I CCATGG 2 cut(s) 562, 817
BspACI CCGC 4 cut(s) 364, 583, 778, 1090
BspANI GGCC 5 cut(s) 367, 768, 777, 908, 1057
BspCNI CTCAG 4 cut(s) 421, 435, 622, 676
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 8 cut(s) 68, 472, 502, 576, 814, 829, 846, 871
BspMI ACCTGC 5 cut(s) 248, 374, 915, 928, 1086
BspPI GGATC 1 cut(s) 122
BspT107I GGYRCC 4 cut(s) 66, 470, 827, 869
BspTNI GGTCTC 3 cut(s) 416, 511, 946
BsrI ACTGG 2 cut(s) 778, 1003
BssECI CCNNGG 9 cut(s) 336, 337, 346, 391, 466, 562, 817, 852, 1189
BssMI GATC 5 cut(s) 85, 127, 180, 634, 1081
BssT1I CCWWGG 5 cut(s) 466, 562, 817, 852, 1189
Bst2UI CCWGG 2 cut(s) 338, 392
Bst4CI ACNGT 9 cut(s) 269, 350, 544, 595, 625, 758, 803, 914, 989
BstC8I GCNNGC 4 cut(s) 24, 115, 766, 922
BstDEI CTNAG 5 cut(s) 429, 443, 609, 684, 894
BstDSI CCRYGG 3 cut(s) 346, 562, 817
BstEII GGTNACC 2 cut(s) 350, 386
BstF5I GGATG 2 cut(s) 585, 1141
BstFNI CGCG 1 cut(s) 234
BstH2I RGCGCY 1 cut(s) 570
BstHHI GCGC 3 cut(s) 234, 383, 569
BstKTI GATC 5 cut(s) 88, 130, 183, 637, 1084
BstMAI GTCTC 4 cut(s) 416, 482, 511, 946
BstMBI GATC 5 cut(s) 85, 127, 180, 634, 1081
BstMCI CGRYCG 1 cut(s) 368
BstMWI GCNNNNNNNGC 3 cut(s) 23, 240, 774
BstNI CCWGG 2 cut(s) 338, 392
BstNSI RCATGY 3 cut(s) 117, 403, 665
BstPAI GACNNNNGTC 1 cut(s) 393
BstPI GGTNACC 2 cut(s) 350, 386
BstSCI CCNGG 3 cut(s) 336, 390, 1057
BstSLI GKGCMC 2 cut(s) 69, 475
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 631, 877, 1099
BstV2I GAAGAC 1 cut(s) 1112
BstX2I RGATCY 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 824
BstYI RGATCY 1 cut(s) 127
BstZI CGGCCG 1 cut(s) 365
BsuRI GGCC 5 cut(s) 367, 768, 777, 908, 1057
BtgI CCRYGG 3 cut(s) 346, 562, 817
BtsCI GGATG 2 cut(s) 585, 1141
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 9 cut(s) 108, 274, 414, 426, 480, 540, 771, 981, 996
BveI ACCTGC 5 cut(s) 248, 374, 915, 928, 1086
Cac8I GCNNGC 4 cut(s) 24, 115, 766, 922
CciI TCATGA 1 cut(s) 177
CfoI GCGC 3 cut(s) 234, 383, 569
Cfr13I GGNCC 2 cut(s) 749, 844
Csp6I GTAC 3 cut(s) 246, 550, 691
CviQI GTAC 3 cut(s) 246, 550, 691
DdeI CTNAG 5 cut(s) 429, 443, 609, 684, 894
DpnI GATC 5 cut(s) 87, 129, 182, 636, 1083
DpnII GATC 5 cut(s) 85, 127, 180, 634, 1081
EaeI YGGCCR 3 cut(s) 365, 775, 906
EagI CGGCCG 1 cut(s) 365
EclXI CGGCCG 1 cut(s) 365
Eco130I CCWWGG 5 cut(s) 466, 562, 817, 852, 1189
Eco24I GRGCYC 2 cut(s) 326, 817
Eco31I GGTCTC 3 cut(s) 416, 511, 946
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 2 cut(s) 749, 844
Eco52I CGGCCG 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 2 cut(s) 119, 962
Eco91I GGTNACC 2 cut(s) 350, 386
EcoO109I RGGNCCY 1 cut(s) 844
EcoO65I GGTNACC 2 cut(s) 350, 386
EcoRII CCWGG 2 cut(s) 336, 390
EcoRV GATATC 1 cut(s) 331
EcoT14I CCWWGG 5 cut(s) 466, 562, 817, 852, 1189
EcoT22I ATGCAT 1 cut(s) 115
EcoT38I GRGCYC 2 cut(s) 326, 817
ErhI CCWWGG 5 cut(s) 466, 562, 817, 852, 1189
Esp3I CGTCTC 1 cut(s) 482
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FauI CCCGC 1 cut(s) 1083
FbaI TGATCA 2 cut(s) 85, 634
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 5 cut(s) 365, 645, 778, 891, 1113
FokI GGATG 2 cut(s) 572, 1148
FriOI GRGCYC 2 cut(s) 326, 817
Fsp4HI GCNGC 5 cut(s) 365, 645, 778, 891, 1113
FspBI CTAG 3 cut(s) 107, 695, 1031
GlaI GCGC 3 cut(s) 233, 382, 568
GluI GCNGC 5 cut(s) 365, 645, 778, 891, 1113
GsaI CCCAGC 1 cut(s) 811
HaeII RGCGCY 1 cut(s) 570
HaeIII GGCC 5 cut(s) 367, 768, 777, 908, 1057
HapII CCGG 3 cut(s) 368, 837, 1058
HhaI GCGC 3 cut(s) 234, 383, 569
Hin6I GCGC 3 cut(s) 232, 381, 567
HinP1I GCGC 3 cut(s) 232, 381, 567
HincII GTYRAC 3 cut(s) 307, 793, 985
HindII GTYRAC 3 cut(s) 307, 793, 985
HinfI GANTC 4 cut(s) 50, 152, 1006, 1021
HpaI GTTAAC 1 cut(s) 307
HpaII CCGG 3 cut(s) 368, 837, 1058
HphI GGTGA 4 cut(s) 344, 398, 770, 1174
Hpy166II GTNNAC 8 cut(s) 42, 58, 307, 346, 749, 793, 859, 985
Hpy188I TCNGA 7 cut(s) 49, 90, 97, 188, 265, 444, 639
Hpy188III TCNNGA 6 cut(s) 131, 178, 278, 683, 962, 1010
Hpy8I GTNNAC 8 cut(s) 42, 58, 307, 346, 749, 793, 859, 985
HpyAV CCTTC 2 cut(s) 43, 841
HpyCH4III ACNGT 9 cut(s) 269, 350, 544, 595, 625, 758, 803, 914, 989
HpyCH4V TGCA 6 cut(s) 101, 113, 159, 294, 930, 937
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 240, 774
HpyF3I CTNAG 5 cut(s) 429, 443, 609, 684, 894
HspAI GCGC 3 cut(s) 232, 381, 567
Ksp22I TGATCA 2 cut(s) 85, 634
KspAI GTTAAC 1 cut(s) 307
Kzo9I GATC 5 cut(s) 85, 127, 180, 634, 1081
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 3 cut(s) 631, 877, 1099
LweI GCATC 5 cut(s) 88, 100, 271, 594, 751
MaeI CTAG 3 cut(s) 107, 695, 1031
MaeIII GTNAC 5 cut(s) 350, 386, 395, 538, 625
MalI GATC 5 cut(s) 87, 129, 182, 636, 1083
MboI GATC 5 cut(s) 85, 127, 180, 634, 1081
MboII GAAGA 2 cut(s) 175, 1117
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 4 cut(s) 69, 326, 475, 817
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 2 cut(s) 249, 375
MluNI TGGCCA 1 cut(s) 908
MlyI GAGTC 2 cut(s) 59, 161
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 7 cut(s) 55, 128, 364, 450, 662, 762, 779
Mox20I TGGCCA 1 cut(s) 908
Mph1103I ATGCAT 1 cut(s) 115
MscI TGGCCA 1 cut(s) 908
MseI TTAA 4 cut(s) 306, 720, 1026, 1074
MslI CAYNNNNRTG 3 cut(s) 176, 759, 822
Msp20I TGGCCA 1 cut(s) 908
MspI CCGG 3 cut(s) 368, 837, 1058
MspR9I CCNGG 3 cut(s) 338, 392, 1059
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 2 cut(s) 338, 392
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 3 cut(s) 23, 240, 774
NciI CCSGG 1 cut(s) 1059
NcoI CCATGG 2 cut(s) 562, 817
NdeII GATC 5 cut(s) 85, 127, 180, 634, 1081
NlaIV GGNNCC 8 cut(s) 68, 472, 502, 576, 814, 829, 846, 871
NmuCI GTSAC 4 cut(s) 350, 386, 395, 538
NsiI ATGCAT 1 cut(s) 115
NspI RCATGY 3 cut(s) 117, 403, 665
PaeI GCATGC 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 119
PagI TCATGA 1 cut(s) 177
PaqCI CACCTGC 3 cut(s) 248, 374, 915
PasI CCCWGGG 1 cut(s) 337
PciI ACATGT 1 cut(s) 399
PfeI GAWTC 2 cut(s) 1006, 1021
PkrI GCNGC 5 cut(s) 366, 646, 779, 892, 1114
PleI GAGTC 2 cut(s) 58, 160
PpsI GAGTC 2 cut(s) 58, 160
PpuMI RGGWCCY 1 cut(s) 844
PscI ACATGT 1 cut(s) 399
PshAI GACNNNNGTC 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 844
Psp6I CCWGG 2 cut(s) 336, 390
PspEI GGTNACC 2 cut(s) 350, 386
PspFI CCCAGC 1 cut(s) 807
PspGI CCWGG 2 cut(s) 336, 390
PspN4I GGNNCC 8 cut(s) 68, 472, 502, 576, 814, 829, 846, 871
PspPI GGNCC 2 cut(s) 749, 844
PspPPI RGGWCCY 1 cut(s) 844
PspXI VCTCGAGB 1 cut(s) 119
PsuI RGATCY 1 cut(s) 127
RsaI GTAC 3 cut(s) 247, 551, 692
RsaNI GTAC 3 cut(s) 246, 550, 691
RseI CAYNNNNRTG 3 cut(s) 176, 759, 822
SaqAI TTAA 4 cut(s) 306, 720, 1026, 1074
SatI GCNGC 5 cut(s) 365, 645, 778, 891, 1113
Sau3AI GATC 5 cut(s) 85, 127, 180, 634, 1081
Sau96I GGNCC 2 cut(s) 749, 844
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 2 cut(s) 59, 161
ScrFI CCNGG 3 cut(s) 338, 392, 1059
SduI GDGCHC 4 cut(s) 69, 326, 475, 817
SfaNI GCATC 5 cut(s) 88, 100, 271, 594, 751
SfiI GGCCNNNNNGGCC 1 cut(s) 774
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 2 cut(s) 749, 844
SlaI CTCGAG 1 cut(s) 119
SmiMI CAYNNNNRTG 3 cut(s) 176, 759, 822
SmlI CTYRAG 2 cut(s) 119, 1085
SmoI CTYRAG 2 cut(s) 119, 1085
SpeI ACTAGT 1 cut(s) 1030
SphI GCATGC 1 cut(s) 117
Sse9I AATT 2 cut(s) 249, 375
SsiI CCGC 4 cut(s) 364, 583, 778, 1090
SspMI CTAG 3 cut(s) 107, 695, 1031
StyD4I CCNGG 3 cut(s) 336, 390, 1057
StyI CCWWGG 5 cut(s) 466, 562, 817, 852, 1189
TaaI ACNGT 9 cut(s) 269, 350, 544, 595, 625, 758, 803, 914, 989
TaqI TCGA 2 cut(s) 120, 1047
TasI AATT 2 cut(s) 249, 375
TatI WGTACW 1 cut(s) 549
TauI GCSGC 2 cut(s) 367, 780
TfiI GAWTC 2 cut(s) 1006, 1021
Tru1I TTAA 4 cut(s) 306, 720, 1026, 1074
Tru9I TTAA 4 cut(s) 306, 720, 1026, 1074
TscAI CASTG 9 cut(s) 108, 274, 421, 433, 487, 547, 778, 981, 1003
TseFI GTSAC 4 cut(s) 350, 386, 395, 538
TseI GCWGC 3 cut(s) 644, 890, 1112
Tsp45I GTSAC 4 cut(s) 350, 386, 395, 538
TspRI CASTG 9 cut(s) 108, 274, 421, 433, 487, 547, 778, 981, 1003
VpaK11BI GGWCC 2 cut(s) 749, 844
XceI RCATGY 3 cut(s) 117, 403, 665
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 208
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 858
XspI CTAG 3 cut(s) 107, 695, 1031
ZrmI AGTACT 1 cut(s) 551
Zsp2I ATGCAT 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.