Rroxscaffold_6G00421180

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002178
Physical Location & Seq
Reverse (-)
42417098 .. 42419757
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421180.1

Sequence Viewer

Length: 1167 bp
ATGGACACCTTCCTCTTCACCTCTGAGTCTGTGAATGAGGGACACCCCGATAAGCTCTGTGATCAAATTTCCGATGCAGTGCTTGATGCCTGCCTTGAACAAGATCCTGACAGCAAAGTTGCCTGTGAGACCTGCACCAAGACCAACATGGTCATGGTCTTCGGAGAGATCACAACTAAAGCCGAAGTGGACTATGAGAAGATAGTGCGCAACACCTGCCGCAGCATTGGATTTGTTTCGGATGATGTAGGCCTTGATGCTGAAAACTGTAAGGTCCTCGTGAACATTGAGCAACAAAGCCCTGATATAGCTCAGGGTGTGCATGGCCACCTCACCAAGAGGCCTGAGGAGATTGGTGCAGGAGACCAGGGTCACATGTTTGGTTATGCTACTGATGAGACCCCCGAGCTTATGCCTCTAAGCCATGTTCTTGCTACCAAGCTTGGTGCCCGTCTCACTGATGTTCGAAAGAATGGCACTTGCCCTTGGCTGAGACCCGATGGCAAGACTCAAGTCACTGTTGAGTACTACAATGACCATGGTGCTATGGTTCCAATCCGTGTCCACACTGTTCTCATTTCTACTCAGCATGATGAGACTGTTACCAATGATGAAATTGCCGCTGACCTCAAGAAACATGTCATTAAGCCAGTTGTTCCTGAGAAGTACCTAGATGAGAAGACTATCTTCCATCTCAACCCTTCGGGTCGATTTGTGATTGGTGGACCGCACGGTGATGCAGGTCTAACTGGCCGGAAGATCATCATTGACACCTATGGTGGTTGGGGAGCACATGGTGGTGGTGCCTTCTCCGGCAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTAGCCAATGGACTTGCTAGAAGGTGCATAGTGCAGGTCTCATATGCAATTGGTGTACCTGAGCCACTATCGGTGTTTGTTGACACTTATGGGACTGGAAAGATTCCAGACAAGGAGATTCTAAAGTTAGTTAAGGAGGCCTTTGATTTTAGGCCTGGAATGATTACCATTAACTTGGATCTCAAGAGAGGAAGGTATTTGAAAACGGCTGCTTATGGACACTTTGGTAGAGATGACCCTGACTTTTCGTGGGAAGTGGTGAAGTCCTTGAAGTGGGAAAAGGCGTAA

Protein Analysis

388

Amino Acids

42.62

Weight (kDa)

5.74

Isoelectric Point (pI)

24.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 2.2e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 3.3e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 240 - 378 7.3e-63 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
Acc16I TGCGCA 1 cut(s) 209
Acc36I ACCTGC 4 cut(s) 140, 224, 731, 904
AccB1I GGYRCC 3 cut(s) 446, 803, 845
AccI GTMKAC 1 cut(s) 834
AciI CCGC 3 cut(s) 220, 621, 728
AclWI GGATC 2 cut(s) 98, 1065
AcoI YGGCCR 2 cut(s) 325, 751
AcsI RAATTY 1 cut(s) 66
AdeI CACNNNGTG 1 cut(s) 797
AfaI GTAC 3 cut(s) 527, 668, 936
AfiI CCNNNNNNNGG 2 cut(s) 829, 1152
AflIII ACRYGT 2 cut(s) 375, 637
AgsI TTSAA 3 cut(s) 98, 1081, 1150
AjnI CCWGG 2 cut(s) 366, 1033
AluBI AGCT 4 cut(s) 55, 311, 409, 442
AluI AGCT 4 cut(s) 55, 311, 409, 442
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
AlwI GGATC 2 cut(s) 98, 1065
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 6 cut(s) 250, 325, 341, 751, 1017, 1031
ApeKI GCWGC 3 cut(s) 222, 866, 1088
ApoI RAATTY 1 cut(s) 66
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 3 cut(s) 274, 725, 820
AsuHPI GGTGA 4 cut(s) 10, 325, 746, 1150
AsuII TTCGAA 1 cut(s) 466
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 3 cut(s) 274, 725, 820
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 3 cut(s) 446, 803, 845
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 2 cut(s) 151, 686
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 3 cut(s) 234, 853, 1075
BccI CCATC 2 cut(s) 494, 699
BceAI ACGGC 1 cut(s) 1101
BciT130I CCWGG 2 cut(s) 368, 1035
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BfaI CTAG 2 cut(s) 671, 897
BfuAI ACCTGC 4 cut(s) 140, 224, 731, 904
BisI GCNGC 5 cut(s) 220, 223, 621, 867, 1089
BlsI GCNGC 5 cut(s) 221, 224, 622, 868, 1090
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 368, 1035
Bme18I GGWCC 3 cut(s) 274, 725, 820
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 274, 725, 820
BmiI GGNNCC 5 cut(s) 448, 552, 805, 822, 847
BmrFI CCNGG 2 cut(s) 368, 1035
BmsI GCATC 4 cut(s) 64, 76, 247, 727
BoxI GACNNNNGTC 2 cut(s) 369, 512
BpiI GAAGAC 2 cut(s) 151, 686
Bpu10I CCTNAGC 2 cut(s) 312, 939
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 3 cut(s) 495, 614, 1046
BsaI GGTCTC 5 cut(s) 122, 357, 392, 487, 922
BsaJI CCNNGG 4 cut(s) 367, 485, 538, 828
Bsc4I CCNNNNNNNGG 2 cut(s) 829, 1152
Bse1I ACTGG 3 cut(s) 650, 754, 979
Bse21I CCTNAGG 1 cut(s) 345
BseBI CCWGG 2 cut(s) 368, 1035
BseDI CCNNGG 4 cut(s) 367, 485, 538, 828
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 829, 1152
BseMII CTCAG 7 cut(s) 15, 326, 336, 482, 599, 651, 930
BseNI ACTGG 3 cut(s) 650, 754, 979
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 3 cut(s) 234, 853, 1075
BsgI GTGCAG 3 cut(s) 118, 378, 932
BshFI GGCC 6 cut(s) 252, 327, 343, 753, 1019, 1033
BshNI GGYRCC 3 cut(s) 446, 803, 845
BsiHKAI GWGCWC 1 cut(s) 793
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 2 cut(s) 754, 813
BslFI GGGAC 2 cut(s) 54, 985
BslI CCNNNNNNNGG 2 cut(s) 829, 1152
BsmAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 2 cut(s) 54, 985
BsnI GGCC 6 cut(s) 252, 327, 343, 753, 1019, 1033
Bso31I GGTCTC 5 cut(s) 122, 357, 392, 487, 922
BsoBI CYCGRG 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 451, 793
Bsp143I GATC 5 cut(s) 61, 103, 168, 759, 1057
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 3 cut(s) 220, 621, 728
BspANI GGCC 6 cut(s) 252, 327, 343, 753, 1019, 1033
BspCNI CTCAG 7 cut(s) 16, 325, 337, 483, 598, 652, 931
BspLI GGNNCC 5 cut(s) 448, 552, 805, 822, 847
BspMI ACCTGC 4 cut(s) 140, 224, 731, 904
BspPI GGATC 2 cut(s) 98, 1065
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 3 cut(s) 446, 803, 845
BspTNI GGTCTC 5 cut(s) 122, 357, 392, 487, 922
BsrI ACTGG 3 cut(s) 650, 754, 979
BssECI CCNNGG 4 cut(s) 367, 485, 538, 828
BssMI GATC 5 cut(s) 61, 103, 168, 759, 1057
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 3 cut(s) 485, 538, 828
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 368, 1035
Bst4CI ACNGT 5 cut(s) 269, 520, 571, 601, 734
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 91, 864
BstDEI CTNAG 9 cut(s) 24, 312, 345, 419, 491, 585, 660, 870, 939
BstDSI CCRYGG 1 cut(s) 538
BstF5I GGATG 1 cut(s) 247
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 5 cut(s) 64, 106, 171, 762, 1060
BstMAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 922
BstMBI GATC 5 cut(s) 61, 103, 168, 759, 1057
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 2 cut(s) 368, 1035
BstNSI RCATGY 2 cut(s) 379, 641
BstPAI GACNNNNGTC 2 cut(s) 369, 512
BstSCI CCNGG 2 cut(s) 366, 1033
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 3 cut(s) 234, 853, 1075
BstV2I GAAGAC 2 cut(s) 151, 686
BstX2I RGATCY 2 cut(s) 103, 1057
BstXI CCANNNNNNTGG 1 cut(s) 1054
BstYI RGATCY 2 cut(s) 103, 1057
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 6 cut(s) 252, 327, 343, 753, 1019, 1033
BtgI CCRYGG 1 cut(s) 538
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 4 cut(s) 84, 456, 516, 567
BveI ACCTGC 4 cut(s) 140, 224, 731, 904
Cac8I GCNNGC 2 cut(s) 91, 864
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 274, 725, 820
Csp6I GTAC 3 cut(s) 526, 667, 935
CviAII CATG 9 cut(s) 148, 154, 323, 376, 425, 539, 590, 638, 794
CviQI GTAC 3 cut(s) 526, 667, 935
DdeI CTNAG 9 cut(s) 24, 312, 345, 419, 491, 585, 660, 870, 939
DpnI GATC 5 cut(s) 63, 105, 170, 761, 1059
DpnII GATC 5 cut(s) 61, 103, 168, 759, 1057
DraIII CACNNNGTG 1 cut(s) 797
EaeI YGGCCR 2 cut(s) 325, 751
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 485, 538, 828
Eco147I AGGCCT 4 cut(s) 252, 343, 1019, 1033
Eco31I GGTCTC 5 cut(s) 122, 357, 392, 487, 922
Eco47I GGWCC 3 cut(s) 274, 725, 820
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 274, 820
EcoRII CCWGG 2 cut(s) 366, 1033
EcoT14I CCWWGG 3 cut(s) 485, 538, 828
ErhI CCWWGG 3 cut(s) 485, 538, 828
Esp3I CGTCTC 1 cut(s) 458
FaeI CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
FalI AAGNNNNNCTT 4 cut(s) 671, 703, 1004, 1036
FaqI GGGAC 2 cut(s) 54, 985
FatI CATG 9 cut(s) 147, 153, 322, 375, 424, 538, 589, 637, 793
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 5 cut(s) 220, 223, 621, 867, 1089
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 5 cut(s) 220, 223, 621, 867, 1089
FspBI CTAG 2 cut(s) 671, 897
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 1 cut(s) 209
GluI GCNGC 5 cut(s) 220, 223, 621, 867, 1089
HaeIII GGCC 6 cut(s) 252, 327, 343, 753, 1019, 1033
HapII CCGG 2 cut(s) 754, 813
HhaI GCGC 1 cut(s) 210
Hin1II CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 961
HindII GTYRAC 1 cut(s) 961
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 26, 508, 982, 997
HpaII CCGG 2 cut(s) 754, 813
HphI GGTGA 4 cut(s) 10, 325, 746, 1150
Hpy166II GTNNAC 7 cut(s) 190, 283, 565, 725, 835, 935, 961
Hpy188I TCNGA 4 cut(s) 25, 73, 164, 241
Hpy188III TCNNGA 6 cut(s) 107, 280, 631, 659, 986, 1063
Hpy8I GTNNAC 7 cut(s) 190, 283, 565, 725, 835, 935, 961
HpyAV CCTTC 5 cut(s) 19, 711, 817, 894, 1065
HpyCH4III ACNGT 5 cut(s) 269, 520, 571, 601, 734
HpyCH4V TGCA 8 cut(s) 77, 135, 322, 359, 740, 906, 913, 926
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 9 cut(s) 24, 312, 345, 419, 491, 585, 660, 870, 939
Hsp92II CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
HspAI GCGC 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 5 cut(s) 61, 103, 168, 759, 1057
LmnI GCTCC 1 cut(s) 788
Lsp1109I GCAGC 3 cut(s) 234, 853, 1075
LweI GCATC 4 cut(s) 64, 76, 247, 727
MaeI CTAG 2 cut(s) 671, 897
MaeIII GTNAC 3 cut(s) 371, 514, 601
MalI GATC 5 cut(s) 63, 105, 170, 761, 1059
MboI GATC 5 cut(s) 61, 103, 168, 759, 1057
MboII GAAGA 6 cut(s) 7, 151, 211, 679, 691, 769
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 2 cut(s) 103, 1057
MhlI GDGCHC 2 cut(s) 451, 793
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 3 cut(s) 66, 615, 927
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 2 cut(s) 35, 502
Mox20I TGGCCA 1 cut(s) 327
MscI TGGCCA 1 cut(s) 327
MseI TTAA 3 cut(s) 645, 1011, 1050
MslI CAYNNNNRTG 3 cut(s) 152, 735, 798
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 2 cut(s) 754, 813
MspR9I CCNGG 2 cut(s) 368, 1035
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 2 cut(s) 368, 1035
MwoI GCNNNNNNNGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 1 cut(s) 922
NdeII GATC 5 cut(s) 61, 103, 168, 759, 1057
NlaIII CATG 9 cut(s) 151, 157, 326, 379, 428, 542, 593, 641, 797
NlaIV GGNNCC 5 cut(s) 448, 552, 805, 822, 847
NmuCI GTSAC 2 cut(s) 371, 514
NsbI TGCGCA 1 cut(s) 209
NspI RCATGY 2 cut(s) 379, 641
NspV TTCGAA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 224
PceI AGGCCT 4 cut(s) 252, 343, 1019, 1033
PciI ACATGT 2 cut(s) 375, 637
PfeI GAWTC 2 cut(s) 982, 997
PkrI GCNGC 5 cut(s) 221, 224, 622, 868, 1090
PleI GAGTC 2 cut(s) 34, 502
PpsI GAGTC 2 cut(s) 34, 502
PpuMI RGGWCCY 2 cut(s) 274, 820
PscI ACATGT 2 cut(s) 375, 637
PshAI GACNNNNGTC 2 cut(s) 369, 512
Psp5II RGGWCCY 2 cut(s) 274, 820
Psp6I CCWGG 2 cut(s) 366, 1033
PspGI CCWGG 2 cut(s) 366, 1033
PspN4I GGNNCC 5 cut(s) 448, 552, 805, 822, 847
PspPI GGNCC 3 cut(s) 274, 725, 820
PspPPI RGGWCCY 2 cut(s) 274, 820
PsuI RGATCY 2 cut(s) 103, 1057
RsaI GTAC 3 cut(s) 527, 668, 936
RsaNI GTAC 3 cut(s) 526, 667, 935
RseI CAYNNNNRTG 3 cut(s) 152, 735, 798
SaqAI TTAA 3 cut(s) 645, 1011, 1050
SatI GCNGC 5 cut(s) 220, 223, 621, 867, 1089
Sau3AI GATC 5 cut(s) 61, 103, 168, 759, 1057
Sau96I GGNCC 3 cut(s) 274, 725, 820
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 35, 502
ScrFI CCNGG 2 cut(s) 368, 1035
SduI GDGCHC 2 cut(s) 451, 793
SfaNI GCATC 4 cut(s) 64, 76, 247, 727
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 3 cut(s) 274, 725, 820
SmiMI CAYNNNNRTG 3 cut(s) 152, 735, 798
SmlI CTYRAG 3 cut(s) 510, 629, 1061
SmoI CTYRAG 3 cut(s) 510, 629, 1061
Sse9I AATT 3 cut(s) 66, 615, 927
SseBI AGGCCT 4 cut(s) 252, 343, 1019, 1033
SsiI CCGC 3 cut(s) 220, 621, 728
SspMI CTAG 2 cut(s) 671, 897
StuI AGGCCT 4 cut(s) 252, 343, 1019, 1033
StyD4I CCNGG 2 cut(s) 366, 1033
StyI CCWWGG 3 cut(s) 485, 538, 828
TaaI ACNGT 5 cut(s) 269, 520, 571, 601, 734
TaqI TCGA 2 cut(s) 466, 709
TasI AATT 3 cut(s) 66, 615, 927
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 222, 623
TfiI GAWTC 2 cut(s) 982, 997
Tru1I TTAA 3 cut(s) 645, 1011, 1050
Tru9I TTAA 3 cut(s) 645, 1011, 1050
TscAI CASTG 4 cut(s) 84, 463, 523, 574
TseFI GTSAC 2 cut(s) 371, 514
TseI GCWGC 3 cut(s) 222, 866, 1088
Tsp45I GTSAC 2 cut(s) 371, 514
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 4 cut(s) 84, 463, 523, 574
VpaK11BI GGWCC 3 cut(s) 274, 725, 820
XapI RAATTY 1 cut(s) 66
XceI RCATGY 2 cut(s) 379, 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 151
XmiI GTMKAC 1 cut(s) 834
XspI CTAG 2 cut(s) 671, 897
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.