MD09G1292700.v1.1

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002178
Physical Location & Seq
Reverse (-)
37519354 .. 37521908
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1292700.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGCGTAGAATGGAGACTTTCCTATTCACATCTGAATCCGTGAACGAGGGTCACCCCGACAAGCTGTGCGACCAGATCTCTGATGCAGTGCTAGATGCCTGCCTTGCCCAGGACCCTGACAGCAAGGTTGCCTGTGAGACATGCACCAAAACCAACATGGTCATGGTGTTCGGAGAGATCACTACCAAGGCCAATGTTGACTATGAGAAGATCGTCCGTGACACTTGCCGCGCCATTGGATTTGTTTCTGATGATGTGGGCCTTGATGCCGATAACTGCAAGGTCCTGGTTAATATTGAGCAGCAGAGCCCTGATATTGCCCAGGGTGTCCATGGTCACTTTACCAAGCGACCTGAGGAGATTGGTGCTGGTGACCAGGGTCACATGTTTGGTTATGCCACTGATGAAACCCCTGAATTGATGCCCCTTAGCCATGTGCTTTCCACCAAGCTTGGTGCTCGTCTCACCGAGGTTAGGAAGAATGGTACCTGCCCTTGGTTGAGACCCGACGGTAAGACCCAAGTCACCGTGGAGTACTACAACGAAAACGGAGCTATGGTTCCAGTCCGTGTCCACACTGTTCTCATCTCCACCCAGCATGATGAGACTGTCACAAACGATGAGATTGCCGCTGACCTCAAGGAGCATGTCATTAAGCCCGTTGTCCCTGAGAAGTACCTTGACGAGAAGACAATCTTCCACCTTAACCCCTCAGGCCGCTTTGTGATTGGTGGTCCTCATGGTGATGCAGGTCTTACTGGACGCAAGATTATAATTGACACTTATGGTGGATGGGGAGCCCATGGTGGAGGTGCTTTCTCTGGAAAGGACCCAACCAAGGTCGACAGGAGTGGCGCCTACATTGTGAGGCAGGCTGCCAAGAGCATTGTAGCAAATGGGCTTGCTCGCAGGGCCATTGTGCAGGTGTCTTATGCCATCGGTGTGCCGGAGCCCTTGTCAGTTTTCGTTGACACATATGGAACTGGAAAGATCCCCGACAGGGAAATCCTTAAAATTGTGAAGGAGACCTTCGACTTCAGGCCTGGCATGATCACTATCAACCTGGACCTCAAGAGGGGTGGTGGGGGAAGGTTCTTGAAGACAGCTGCCTATGGACACTTTGGAAGGGACGATCCAGATTTCACCTGGGAGGTTGTGAAGCCCCTCAAATGGGAGAAGCCTCAATCTTGA

Protein Analysis

397

Amino Acids

43.46

Weight (kDa)

5.77

Isoelectric Point (pI)

26.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 7 - 104 3.1e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 120 - 241 9.8e-48 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 243 - 384 5.5e-60 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AarI CACCTGC 1 cut(s) 913
Acc36I ACCTGC 3 cut(s) 497, 740, 913
Acc65I GGTACC 1 cut(s) 485
AccB1I GGYRCC 2 cut(s) 485, 854
AccI GTMKAC 1 cut(s) 843
AccII CGCG 1 cut(s) 231
AciI CCGC 3 cut(s) 229, 630, 718
AclWI GGATC 2 cut(s) 985, 1127
AcuI CTGAAG 1 cut(s) 1021
AcyI GRCGYC 1 cut(s) 855
AfaI GTAC 3 cut(s) 487, 536, 677
AfiI CCNNNNNNNGG 8 cut(s) 109, 474, 495, 838, 1000, 1075, 1170, 1171
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 1 cut(s) 1099
AjnI CCWGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
AluBI AGCT 4 cut(s) 64, 451, 554, 1106
AluI AGCT 4 cut(s) 64, 451, 554, 1106
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 6 cut(s) 8, 131, 467, 496, 599, 1019
AlwI GGATC 2 cut(s) 985, 1127
AoxI GGCC 5 cut(s) 189, 259, 715, 912, 1040
ApeKI GCWGC 3 cut(s) 301, 875, 1106
Asp718I GGTACC 1 cut(s) 485
AspLEI GCGC 2 cut(s) 233, 857
AspS9I GGNCC 7 cut(s) 112, 259, 283, 734, 829, 912, 1066
AsuHPI GGTGA 6 cut(s) 44, 383, 457, 517, 755, 1135
AvaII GGWCC 5 cut(s) 112, 283, 734, 829, 1066
AxyI CCTNAGG 2 cut(s) 354, 712
BanI GGYRCC 2 cut(s) 485, 854
BanII GRGCYC 3 cut(s) 311, 802, 954
BbsI GAAGAC 2 cut(s) 695, 1106
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 313, 862, 1093
BccI CCATC 2 cut(s) 786, 944
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
BclI TGATCA 1 cut(s) 1050
BcoDI GTCTC 6 cut(s) 8, 131, 467, 496, 599, 1019
BfaI CTAG 1 cut(s) 92
BfoI RGCGCY 1 cut(s) 858
BfuAI ACCTGC 3 cut(s) 497, 740, 913
BglII AGATCT 1 cut(s) 75
BisI GCNGC 6 cut(s) 229, 302, 630, 718, 876, 1107
BlsI GCNGC 6 cut(s) 230, 303, 631, 719, 877, 1108
BmcAI AGTACT 1 cut(s) 536
Bme1390I CCNGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
Bme18I GGWCC 5 cut(s) 112, 283, 734, 829, 1066
BmgT120I GGNCC 7 cut(s) 112, 259, 283, 734, 829, 912, 1066
BmiI GGNNCC 7 cut(s) 114, 487, 561, 799, 831, 856, 951
BmrFI CCNGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
BmsI GCATC 5 cut(s) 73, 85, 256, 411, 736
BoxI GACNNNNGTC 2 cut(s) 378, 521
BpiI GAAGAC 2 cut(s) 695, 1106
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 2 cut(s) 623, 1055
BsaBI GATNNNNATC 1 cut(s) 1055
BsaHI GRCGYC 1 cut(s) 855
BsaI GGTCTC 2 cut(s) 496, 1019
BsaXI ACNNNNNCTCC 4 cut(s) 543, 573, 941, 971
Bsc4I CCNNNNNNNGG 8 cut(s) 109, 474, 495, 838, 1000, 1075, 1170, 1171
Bse1I ACTGG 3 cut(s) 563, 763, 988
Bse21I CCTNAGG 2 cut(s) 354, 712
Bse8I GATNNNNATC 1 cut(s) 1055
BseBI CCWGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
BseGI GGATG 1 cut(s) 797
BseJI GATNNNNATC 1 cut(s) 1055
BseLI CCNNNNNNNGG 8 cut(s) 109, 474, 495, 838, 1000, 1075, 1170, 1171
BseMII CTCAG 3 cut(s) 345, 660, 726
BseNI ACTGG 3 cut(s) 563, 763, 988
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 3 cut(s) 313, 862, 1093
BseYI CCCAGC 1 cut(s) 594
BsgI GTGCAG 1 cut(s) 941
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 191, 261, 717, 914, 1042
BshNI GGYRCC 2 cut(s) 485, 854
BsiHKAI GWGCWC 1 cut(s) 460
BsiSI CCGG 1 cut(s) 947
BslFI GGGAC 2 cut(s) 650, 1142
BslI CCNNNNNNNGG 8 cut(s) 109, 474, 495, 838, 1000, 1075, 1170, 1171
BsmAI GTCTC 6 cut(s) 8, 131, 467, 496, 599, 1019
BsmBI CGTCTC 1 cut(s) 467
BsmFI GGGAC 2 cut(s) 650, 1142
BsnI GGCC 5 cut(s) 191, 261, 717, 914, 1042
Bso31I GGTCTC 2 cut(s) 496, 1019
Bsp1286I GDGCHC 4 cut(s) 311, 460, 802, 954
Bsp143I GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
Bsp19I CCATGG 2 cut(s) 331, 802
BspACI CCGC 3 cut(s) 229, 630, 718
BspANI GGCC 5 cut(s) 191, 261, 717, 914, 1042
BspCNI CTCAG 3 cut(s) 346, 661, 725
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 7 cut(s) 114, 487, 561, 799, 831, 856, 951
BspMI ACCTGC 3 cut(s) 497, 740, 913
BspPI GGATC 2 cut(s) 985, 1127
BspT107I GGYRCC 2 cut(s) 485, 854
BspTNI GGTCTC 2 cut(s) 496, 1019
BsrI ACTGG 3 cut(s) 563, 763, 988
BssMI GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
BssNI GRCGYC 1 cut(s) 855
BssT1I CCWWGG 5 cut(s) 186, 331, 494, 802, 837
Bst2UI CCWGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
Bst4CI ACNGT 4 cut(s) 512, 529, 580, 610
BstACI GRCGYC 1 cut(s) 855
BstC8I GCNNGC 4 cut(s) 100, 873, 903, 907
BstDEI CTNAG 4 cut(s) 354, 428, 669, 712
BstDSI CCRYGG 3 cut(s) 331, 528, 802
BstEII GGTNACC 2 cut(s) 50, 371
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 797
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 858
BstHHI GCGC 2 cut(s) 233, 857
BstKTI GATC 6 cut(s) 78, 180, 213, 993, 1053, 1135
BstMAI GTCTC 6 cut(s) 8, 131, 467, 496, 599, 1019
BstMBI GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
BstMWI GCNNNNNNNGC 2 cut(s) 104, 911
BstNI CCWGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
BstNSI RCATGY 3 cut(s) 144, 388, 650
BstPAI GACNNNNGTC 2 cut(s) 378, 521
BstPI GGTNACC 2 cut(s) 50, 371
BstSCI CCNGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 3 cut(s) 313, 862, 1093
BstV2I GAAGAC 2 cut(s) 695, 1106
BstX2I RGATCY 2 cut(s) 75, 990
BstYI RGATCY 2 cut(s) 75, 990
Bsu36I CCTNAGG 2 cut(s) 354, 712
BsuRI GGCC 5 cut(s) 191, 261, 717, 914, 1042
BtgI CCRYGG 3 cut(s) 331, 528, 802
BtsCI GGATG 1 cut(s) 797
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 3 cut(s) 93, 399, 576
BveI ACCTGC 3 cut(s) 497, 740, 913
Cac8I GCNNGC 4 cut(s) 100, 873, 903, 907
CfoI GCGC 2 cut(s) 233, 857
Cfr13I GGNCC 7 cut(s) 112, 259, 283, 734, 829, 912, 1066
CseI GACGC 1 cut(s) 771
Csp6I GTAC 3 cut(s) 486, 535, 676
CspCI CAANNNNNGTGG 4 cut(s) 433, 468, 1060, 1095
CviQI GTAC 3 cut(s) 486, 535, 676
DdeI CTNAG 4 cut(s) 354, 428, 669, 712
DinI GGCGCC 1 cut(s) 856
DpnI GATC 6 cut(s) 77, 179, 212, 992, 1052, 1134
DpnII GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
Eco130I CCWWGG 5 cut(s) 186, 331, 494, 802, 837
Eco147I AGGCCT 1 cut(s) 1042
Eco24I GRGCYC 3 cut(s) 311, 802, 954
Eco31I GGTCTC 2 cut(s) 496, 1019
Eco47I GGWCC 5 cut(s) 112, 283, 734, 829, 1066
Eco57I CTGAAG 1 cut(s) 1021
Eco81I CCTNAGG 2 cut(s) 354, 712
Eco91I GGTNACC 2 cut(s) 50, 371
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 3 cut(s) 112, 283, 829
EcoO65I GGTNACC 2 cut(s) 50, 371
EcoRII CCWGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
EcoT14I CCWWGG 5 cut(s) 186, 331, 494, 802, 837
EcoT38I GRGCYC 3 cut(s) 311, 802, 954
EgeI GGCGCC 1 cut(s) 856
EheI GGCGCC 1 cut(s) 856
ErhI CCWWGG 5 cut(s) 186, 331, 494, 802, 837
Esp3I CGTCTC 1 cut(s) 467
FalI AAGNNNNNCTT 4 cut(s) 680, 712, 1013, 1045
FaqI GGGAC 2 cut(s) 650, 1142
FauNDI CATATG 1 cut(s) 976
FbaI TGATCA 1 cut(s) 1050
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 6 cut(s) 229, 302, 630, 718, 876, 1107
FokI GGATG 1 cut(s) 804
FriOI GRGCYC 3 cut(s) 311, 802, 954
Fsp4HI GCNGC 6 cut(s) 229, 302, 630, 718, 876, 1107
FspBI CTAG 1 cut(s) 92
GlaI GCGC 2 cut(s) 232, 856
GluI GCNGC 6 cut(s) 229, 302, 630, 718, 876, 1107
GsaI CCCAGC 1 cut(s) 598
HaeII RGCGCY 1 cut(s) 858
HaeIII GGCC 5 cut(s) 191, 261, 717, 914, 1042
HapII CCGG 1 cut(s) 947
HgaI GACGC 1 cut(s) 771
HhaI GCGC 2 cut(s) 233, 857
Hin1I GRCGYC 1 cut(s) 855
Hin6I GCGC 2 cut(s) 231, 855
HinP1I GCGC 2 cut(s) 231, 855
HincII GTYRAC 3 cut(s) 199, 844, 970
HindII GTYRAC 3 cut(s) 199, 844, 970
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 1 cut(s) 35
HpaII CCGG 1 cut(s) 947
HphI GGTGA 6 cut(s) 44, 383, 457, 517, 755, 1135
Hpy166II GTNNAC 5 cut(s) 43, 199, 574, 844, 970
Hpy188I TCNGA 4 cut(s) 34, 82, 173, 250
Hpy188III TCNNGA 5 cut(s) 822, 1072, 1096, 1136, 1188
Hpy8I GTNNAC 5 cut(s) 43, 199, 574, 844, 970
Hpy99I CGWCG 1 cut(s) 512
HpyAV CCTTC 4 cut(s) 1015, 1039, 1083, 1119
HpyCH4III ACNGT 4 cut(s) 512, 529, 580, 610
HpyCH4V TGCA 5 cut(s) 86, 144, 279, 749, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 911
HpyF3I CTNAG 4 cut(s) 354, 428, 669, 712
Hsp92I GRCGYC 1 cut(s) 855
HspAI GCGC 2 cut(s) 231, 855
KasI GGCGCC 1 cut(s) 854
KpnI GGTACC 1 cut(s) 489
Ksp22I TGATCA 1 cut(s) 1050
Kzo9I GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
LmnI GCTCC 4 cut(s) 551, 643, 797, 949
Lsp1109I GCAGC 3 cut(s) 313, 862, 1093
LweI GCATC 5 cut(s) 73, 85, 256, 411, 736
MaeI CTAG 1 cut(s) 92
MaeIII GTNAC 7 cut(s) 50, 218, 335, 371, 380, 523, 610
MalI GATC 6 cut(s) 77, 179, 212, 992, 1052, 1134
MboI GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
MboII GAAGA 5 cut(s) 220, 490, 688, 700, 1111
MflI RGATCY 2 cut(s) 75, 990
MhlI GDGCHC 4 cut(s) 311, 460, 802, 954
MluCI AATT 3 cut(s) 416, 774, 1014
Mly113I GGCGCC 1 cut(s) 855
MseI TTAA 4 cut(s) 291, 654, 705, 1011
MslI CAYNNNNRTG 3 cut(s) 161, 744, 941
MspA1I CMGCKG 2 cut(s) 632, 1106
MspI CCGG 1 cut(s) 947
MspR9I CCNGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
MvaI CCWGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 104, 911
NarI GGCGCC 1 cut(s) 855
NcoI CCATGG 2 cut(s) 331, 802
NdeI CATATG 1 cut(s) 976
NdeII GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
NlaIV GGNNCC 7 cut(s) 114, 487, 561, 799, 831, 856, 951
NmuCI GTSAC 7 cut(s) 50, 218, 335, 371, 380, 523, 610
NspI RCATGY 3 cut(s) 144, 388, 650
PaqCI CACCTGC 1 cut(s) 913
PasI CCCWGGG 1 cut(s) 322
PceI AGGCCT 1 cut(s) 1042
PciI ACATGT 1 cut(s) 384
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 6 cut(s) 230, 303, 631, 719, 877, 1108
PluTI GGCGCC 1 cut(s) 858
PpuMI RGGWCCY 3 cut(s) 112, 283, 829
PscI ACATGT 1 cut(s) 384
PshAI GACNNNNGTC 2 cut(s) 378, 521
PsiI TTATAA 1 cut(s) 773
Psp5II RGGWCCY 3 cut(s) 112, 283, 829
Psp6I CCWGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
PspEI GGTNACC 2 cut(s) 50, 371
PspFI CCCAGC 1 cut(s) 594
PspGI CCWGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
PspN4I GGNNCC 7 cut(s) 114, 487, 561, 799, 831, 856, 951
PspPI GGNCC 7 cut(s) 112, 259, 283, 734, 829, 912, 1066
PspPPI RGGWCCY 3 cut(s) 112, 283, 829
PsuI RGATCY 2 cut(s) 75, 990
PvuII CAGCTG 1 cut(s) 1106
RsaI GTAC 3 cut(s) 487, 536, 677
RsaNI GTAC 3 cut(s) 486, 535, 676
RseI CAYNNNNRTG 3 cut(s) 161, 744, 941
SalI GTCGAC 1 cut(s) 842
SaqAI TTAA 4 cut(s) 291, 654, 705, 1011
SatI GCNGC 6 cut(s) 229, 302, 630, 718, 876, 1107
Sau3AI GATC 6 cut(s) 75, 177, 210, 990, 1050, 1132
Sau96I GGNCC 7 cut(s) 112, 259, 283, 734, 829, 912, 1066
ScaI AGTACT 1 cut(s) 536
ScrFI CCNGG 7 cut(s) 110, 287, 323, 377, 1044, 1064, 1147
SduI GDGCHC 4 cut(s) 311, 460, 802, 954
SfaNI GCATC 5 cut(s) 73, 85, 256, 411, 736
SfoI GGCGCC 1 cut(s) 856
SinI GGWCC 5 cut(s) 112, 283, 734, 829, 1066
SmiMI CAYNNNNRTG 3 cut(s) 161, 744, 941
SmlI CTYRAG 2 cut(s) 638, 1070
SmoI CTYRAG 2 cut(s) 638, 1070
Sse9I AATT 3 cut(s) 416, 774, 1014
SseBI AGGCCT 1 cut(s) 1042
SsiI CCGC 3 cut(s) 229, 630, 718
SspDI GGCGCC 1 cut(s) 854
SspI AATATT 1 cut(s) 295
SspMI CTAG 1 cut(s) 92
StuI AGGCCT 1 cut(s) 1042
StyD4I CCNGG 7 cut(s) 108, 285, 321, 375, 1042, 1062, 1145
StyI CCWWGG 5 cut(s) 186, 331, 494, 802, 837
TaaI ACNGT 4 cut(s) 512, 529, 580, 610
TaqI TCGA 2 cut(s) 843, 1032
TasI AATT 3 cut(s) 416, 774, 1014
TatI WGTACW 1 cut(s) 534
TauI GCSGC 3 cut(s) 231, 632, 720
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 4 cut(s) 291, 654, 705, 1011
Tru9I TTAA 4 cut(s) 291, 654, 705, 1011
TscAI CASTG 3 cut(s) 93, 406, 583
TseFI GTSAC 7 cut(s) 50, 218, 335, 371, 380, 523, 610
TseI GCWGC 3 cut(s) 301, 875, 1106
Tsp45I GTSAC 7 cut(s) 50, 218, 335, 371, 380, 523, 610
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 4 cut(s) 28, 206, 557, 564
TspRI CASTG 3 cut(s) 93, 406, 583
VpaK11BI GGWCC 5 cut(s) 112, 283, 734, 829, 1066
XagI CCTNNNNNAGG 1 cut(s) 107
XceI RCATGY 3 cut(s) 144, 388, 650
XcmI CCANNNNNNNNNTGG 4 cut(s) 154, 160, 329, 1143
XmiI GTMKAC 1 cut(s) 843
XspI CTAG 1 cut(s) 92
ZrmI AGTACT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.