pycom12g18740

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002178
Physical Location & Seq
Reverse (-)
20852514 .. 20853539
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18740.1

Sequence Viewer

Length: 1026 bp
ATGGTCATGGTCTTCGGAGAGATTACAACCAAAGCCAAAGTGGACTACGAGAAGATTGTGCGAGATACCTGCCGTACAATTGGATTTGTTTCAGATGATGTTGGTCTTGATGCTGACAACTGCAAGGTCTTGGTTAACATCGAGCAACAGAGCCCTGATATTGCCCAGGGCGTTCACGGTCACTTGACCAAGCGGCCTGAGGACATTGGTGCGGGTGACCAGGGTCACATGTTTGGTTATGCCACTGATGAGACCCCTGAGCTTATGCCTCTGAGCCATGTTCTTGCCACCAAGCTTGGTGCCCGTCTCACTGAAGTTCGCAAGAATGGAACCTTGCCGTGGCTGAGACCTGATGGCAAGACGCAAGTCACTGTTGAATACTACAATGACCATGGCGCTATGGTTCCAATCCGTGTCCACACGGTTCTCATTTCAACTCAGCATGACGAAACTGTTACCAACGACGAGATTGCTGCTGACCTCAAAGAGCATGTTATCAAGCCTGTTGTTCCTGAGAAGTACCTAGACGAGAAGACTATTTTCCATCTCAACCCATCAGGTCGATTTGTCATTGGTGGACCGCACGGTGATGCTGGCCTCACTGGCCGCAAAATCATCATTGACACCTACGGTGGTTGGGGAGCTCATGGTGGTGGTGCCTTCTCCGGCAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTCGCCAATGGTCTTGCTCGCAGGTGCATAGTGCAGGTCTCCTATGCCATTGGTGTTCCCGAGCCACTATCAGTGTTTGTTGACAGTTATGGCACCGGAAAGATTCCTGACAAGGAGATTCTTAAACTAGTAAAGGAGAACTTTGATTTCAGGCCCGGGATGATTGCGATTAACCTCGATCTCAAGCGGGGCAGGTACTTGAAAACGGCTGCTTACGGACACTTCGGTAGGGATGACCCTGACTTCTCATGGGAAGTGGTGAAGCCATTGAAGTGGGAAAAGCCCCAAGAGTAG

Protein Analysis

342

Amino Acids

37.57

Weight (kDa)

6.32

Isoelectric Point (pI)

19.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 1 - 52 3.6e-12 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 68 - 189 6.2e-48 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 191 - 329 7.8e-63 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 744
Acc36I ACCTGC 4 cut(s) 77, 744, 757, 915
AccB1I GGYRCC 4 cut(s) 299, 656, 698, 824
AccI GTMKAC 1 cut(s) 687
AciI CCGC 5 cut(s) 193, 212, 581, 607, 919
AcoI YGGCCR 1 cut(s) 604
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 3 cut(s) 76, 521, 929
AfiI CCNNNNNNNGG 2 cut(s) 339, 682
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 4 cut(s) 377, 435, 934, 1003
AhlI ACTAGT 1 cut(s) 859
AjnI CCWGG 2 cut(s) 165, 219
AluBI AGCT 3 cut(s) 262, 295, 644
AluI AGCT 3 cut(s) 262, 295, 644
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 4 cut(s) 245, 311, 340, 775
Ama87I CYCGRG 2 cut(s) 791, 887
AoxI GGCC 4 cut(s) 194, 595, 604, 884
ApeKI GCWGC 3 cut(s) 473, 719, 941
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 578, 673, 885
AsuC2I CCSGG 2 cut(s) 888, 889
AsuHPI GGTGA 3 cut(s) 227, 599, 1003
AvaI CYCGRG 2 cut(s) 791, 887
AvaII GGWCC 2 cut(s) 578, 673
AxyI CCTNAGG 1 cut(s) 198
BaeGI GKGCMC 1 cut(s) 304
BanI GGYRCC 4 cut(s) 299, 656, 698, 824
BanII GRGCYC 2 cut(s) 155, 646
BbsI GAAGAC 2 cut(s) 4, 539
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 3 cut(s) 460, 706, 928
BccI CCATC 3 cut(s) 347, 552, 562
BceAI ACGGC 3 cut(s) 57, 322, 954
BcgI CGANNNNNNTGC 6 cut(s) 51, 85, 452, 455, 486, 489
BciT130I CCWGG 2 cut(s) 167, 221
BcnI CCSGG 2 cut(s) 888, 889
BcoDI GTCTC 4 cut(s) 245, 311, 340, 775
BcuI ACTAGT 1 cut(s) 859
BfaI CTAG 2 cut(s) 524, 860
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 4 cut(s) 77, 744, 757, 915
BglI GCCNNNNNGGC 1 cut(s) 603
BisI GCNGC 5 cut(s) 194, 474, 607, 720, 942
BlsI GCNGC 5 cut(s) 195, 475, 608, 721, 943
Bme1390I CCNGG 4 cut(s) 167, 221, 888, 889
Bme18I GGWCC 2 cut(s) 578, 673
BmeT110I CYCGRG 2 cut(s) 791, 887
BmgT120I GGNCC 3 cut(s) 578, 673, 885
BmiI GGNNCC 7 cut(s) 301, 331, 405, 658, 675, 700, 826
BmrFI CCNGG 4 cut(s) 167, 221, 888, 889
BmsI GCATC 2 cut(s) 100, 580
BoxI GACNNNNGTC 2 cut(s) 222, 365
BpiI GAAGAC 2 cut(s) 4, 539
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 2 cut(s) 888, 889
BsaI GGTCTC 3 cut(s) 245, 340, 775
BsaJI CCNNGG 7 cut(s) 165, 166, 220, 338, 391, 681, 887
BsaWI WCCGGW 1 cut(s) 827
Bsc4I CCNNNNNNNGG 2 cut(s) 339, 682
Bse1I ACTGG 1 cut(s) 607
Bse21I CCTNAGG 1 cut(s) 198
BseBI CCWGG 2 cut(s) 167, 221
BseDI CCNNGG 7 cut(s) 165, 166, 220, 338, 391, 681, 887
BseGI GGATG 2 cut(s) 897, 970
BseLI CCNNNNNNNGG 2 cut(s) 339, 682
BseMII CTCAG 6 cut(s) 189, 249, 263, 335, 452, 504
BseNI ACTGG 1 cut(s) 607
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 3 cut(s) 460, 706, 928
BsgI GTGCAG 1 cut(s) 785
BshFI GGCC 4 cut(s) 196, 597, 606, 886
BshNI GGYRCC 4 cut(s) 299, 656, 698, 824
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 2 cut(s) 791, 887
BsiSI CCGG 3 cut(s) 666, 828, 888
BslI CCNNNNNNNGG 2 cut(s) 339, 682
BsmAI GTCTC 4 cut(s) 245, 311, 340, 775
BsmBI CGTCTC 1 cut(s) 311
BsnI GGCC 4 cut(s) 196, 597, 606, 886
Bso31I GGTCTC 3 cut(s) 245, 340, 775
BsoBI CYCGRG 2 cut(s) 791, 887
Bsp1286I GDGCHC 3 cut(s) 155, 304, 646
Bsp143I GATC 1 cut(s) 910
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 5 cut(s) 193, 212, 581, 607, 919
BspANI GGCC 4 cut(s) 196, 597, 606, 886
BspCNI CTCAG 6 cut(s) 190, 250, 264, 336, 451, 505
BspLI GGNNCC 7 cut(s) 301, 331, 405, 658, 675, 700, 826
BspMI ACCTGC 4 cut(s) 77, 744, 757, 915
BspT107I GGYRCC 4 cut(s) 299, 656, 698, 824
BspTNI GGTCTC 3 cut(s) 245, 340, 775
BsrI ACTGG 1 cut(s) 607
BssECI CCNNGG 7 cut(s) 165, 166, 220, 338, 391, 681, 887
BssMI GATC 1 cut(s) 910
BssT1I CCWWGG 2 cut(s) 391, 681
Bst2UI CCWGG 2 cut(s) 167, 221
Bst4CI ACNGT 7 cut(s) 179, 373, 424, 454, 587, 632, 818
BstC8I GCNNGC 3 cut(s) 595, 717, 751
BstDEI CTNAG 7 cut(s) 198, 258, 272, 344, 438, 513, 723
BstDSI CCRYGG 2 cut(s) 338, 391
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 2 cut(s) 897, 970
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 1 cut(s) 913
BstMAI GTCTC 4 cut(s) 245, 311, 340, 775
BstMBI GATC 1 cut(s) 910
BstMWI GCNNNNNNNGC 1 cut(s) 603
BstNI CCWGG 2 cut(s) 167, 221
BstNSI RCATGY 2 cut(s) 232, 494
BstPAI GACNNNNGTC 2 cut(s) 222, 365
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 4 cut(s) 165, 219, 886, 887
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 3 cut(s) 460, 706, 928
BstV2I GAAGAC 2 cut(s) 4, 539
BstXI CCANNNNNNTGG 1 cut(s) 1005
Bsu36I CCTNAGG 1 cut(s) 198
BsuRI GGCC 4 cut(s) 196, 597, 606, 886
BtgI CCRYGG 2 cut(s) 338, 391
BtsCI GGATG 2 cut(s) 897, 970
BtsIMutI CAGTG 5 cut(s) 243, 309, 369, 600, 810
BveI ACCTGC 4 cut(s) 77, 744, 757, 915
Cac8I GCNNGC 3 cut(s) 595, 717, 751
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 3 cut(s) 578, 673, 885
Cfr9I CCCGGG 1 cut(s) 887
CseI GACGC 1 cut(s) 370
Csp6I GTAC 3 cut(s) 75, 520, 928
CspCI CAANNNNNGTGG 2 cut(s) 277, 312
CviAII CATG 8 cut(s) 7, 229, 278, 392, 443, 491, 647, 981
CviQI GTAC 3 cut(s) 75, 520, 928
DdeI CTNAG 7 cut(s) 198, 258, 272, 344, 438, 513, 723
DpnI GATC 1 cut(s) 912
DpnII GATC 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 604
Ecl136II GAGCTC 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 391, 681
Eco24I GRGCYC 2 cut(s) 155, 646
Eco31I GGTCTC 3 cut(s) 245, 340, 775
Eco47I GGWCC 2 cut(s) 578, 673
Eco53kI GAGCTC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 333
Eco81I CCTNAGG 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 791, 887
Eco91I GGTNACC 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 644
EcoO109I RGGNCCY 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 2 cut(s) 165, 219
EcoT14I CCWWGG 2 cut(s) 391, 681
EcoT38I GRGCYC 2 cut(s) 155, 646
ErhI CCWWGG 2 cut(s) 391, 681
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 8 cut(s) 10, 232, 281, 395, 446, 494, 650, 984
FalI AAGNNNNNCTT 2 cut(s) 857, 889
FatI CATG 8 cut(s) 6, 228, 277, 391, 442, 490, 646, 980
FauI CCCGC 2 cut(s) 205, 912
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 5 cut(s) 194, 474, 607, 720, 942
FokI GGATG 2 cut(s) 904, 977
FriOI GRGCYC 2 cut(s) 155, 646
Fsp4HI GCNGC 5 cut(s) 194, 474, 607, 720, 942
FspBI CTAG 2 cut(s) 524, 860
GlaI GCGC 1 cut(s) 397
GluI GCNGC 5 cut(s) 194, 474, 607, 720, 942
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 4 cut(s) 196, 597, 606, 886
HapII CCGG 3 cut(s) 666, 828, 888
HgaI GACGC 1 cut(s) 370
HhaI GCGC 1 cut(s) 398
Hin1II CATG 8 cut(s) 10, 232, 281, 395, 446, 494, 650, 984
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 2 cut(s) 136, 814
HindII GTYRAC 2 cut(s) 136, 814
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 2 cut(s) 835, 850
HpaI GTTAAC 1 cut(s) 136
HpaII CCGG 3 cut(s) 666, 828, 888
HphI GGTGA 3 cut(s) 227, 599, 1003
Hpy166II GTNNAC 7 cut(s) 43, 136, 175, 418, 578, 688, 814
Hpy188I TCNGA 3 cut(s) 17, 94, 273
Hpy188III TCNNGA 4 cut(s) 107, 512, 791, 839
Hpy8I GTNNAC 7 cut(s) 43, 136, 175, 418, 578, 688, 814
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 7 cut(s) 179, 373, 424, 454, 587, 632, 818
HpyCH4V TGCA 3 cut(s) 123, 759, 766
HpyF10VI GCNNNNNNNGC 1 cut(s) 603
HpyF3I CTNAG 7 cut(s) 198, 258, 272, 344, 438, 513, 723
Hsp92II CATG 8 cut(s) 10, 232, 281, 395, 446, 494, 650, 984
HspAI GCGC 1 cut(s) 396
KspAI GTTAAC 1 cut(s) 136
Kzo9I GATC 1 cut(s) 910
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 3 cut(s) 460, 706, 928
LweI GCATC 2 cut(s) 100, 580
MaeI CTAG 2 cut(s) 524, 860
MaeIII GTNAC 5 cut(s) 179, 215, 224, 367, 454
MalI GATC 1 cut(s) 912
MboI GATC 1 cut(s) 910
MboII GAAGA 3 cut(s) 4, 64, 544
MfeI CAATTG 1 cut(s) 78
MhlI GDGCHC 3 cut(s) 155, 304, 646
MluCI AATT 1 cut(s) 78
MnlI CCTC 5 cut(s) 193, 279, 491, 608, 917
MseI TTAA 3 cut(s) 135, 855, 903
MslI CAYNNNNRTG 3 cut(s) 588, 651, 1003
MspI CCGG 3 cut(s) 666, 828, 888
MspR9I CCNGG 4 cut(s) 167, 221, 888, 889
MunI CAATTG 1 cut(s) 78
MvaI CCWGG 2 cut(s) 167, 221
MwoI GCNNNNNNNGC 1 cut(s) 603
NciI CCSGG 2 cut(s) 888, 889
NcoI CCATGG 1 cut(s) 391
NdeII GATC 1 cut(s) 910
NlaIII CATG 8 cut(s) 10, 232, 281, 395, 446, 494, 650, 984
NlaIV GGNNCC 7 cut(s) 301, 331, 405, 658, 675, 700, 826
NmuCI GTSAC 4 cut(s) 179, 215, 224, 367
NspI RCATGY 2 cut(s) 232, 494
PaqCI CACCTGC 1 cut(s) 744
PasI CCCWGGG 1 cut(s) 166
PciI ACATGT 1 cut(s) 228
PfeI GAWTC 2 cut(s) 835, 850
PkrI GCNGC 5 cut(s) 195, 475, 608, 721, 943
PpuMI RGGWCCY 1 cut(s) 673
PscI ACATGT 1 cut(s) 228
PshAI GACNNNNGTC 2 cut(s) 222, 365
Psp124BI GAGCTC 1 cut(s) 646
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 165, 219
PspEI GGTNACC 1 cut(s) 215
PspGI CCWGG 2 cut(s) 165, 219
PspN4I GGNNCC 7 cut(s) 301, 331, 405, 658, 675, 700, 826
PspPI GGNCC 3 cut(s) 578, 673, 885
PspPPI RGGWCCY 1 cut(s) 673
RsaI GTAC 3 cut(s) 76, 521, 929
RsaNI GTAC 3 cut(s) 75, 520, 928
RseI CAYNNNNRTG 3 cut(s) 588, 651, 1003
SacI GAGCTC 1 cut(s) 646
SaqAI TTAA 3 cut(s) 135, 855, 903
SatI GCNGC 5 cut(s) 194, 474, 607, 720, 942
Sau3AI GATC 1 cut(s) 910
Sau96I GGNCC 3 cut(s) 578, 673, 885
ScrFI CCNGG 4 cut(s) 167, 221, 888, 889
SduI GDGCHC 3 cut(s) 155, 304, 646
SfaNI GCATC 2 cut(s) 100, 580
SfiI GGCCNNNNNGGCC 1 cut(s) 603
SinI GGWCC 2 cut(s) 578, 673
SmaI CCCGGG 1 cut(s) 889
SmiMI CAYNNNNRTG 3 cut(s) 588, 651, 1003
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SpeI ACTAGT 1 cut(s) 859
Sse9I AATT 1 cut(s) 78
SsiI CCGC 5 cut(s) 193, 212, 581, 607, 919
SspMI CTAG 2 cut(s) 524, 860
SstI GAGCTC 1 cut(s) 646
StyD4I CCNGG 4 cut(s) 165, 219, 886, 887
StyI CCWWGG 2 cut(s) 391, 681
TaaI ACNGT 7 cut(s) 179, 373, 424, 454, 587, 632, 818
TaqI TCGA 3 cut(s) 141, 562, 909
TasI AATT 1 cut(s) 78
TauI GCSGC 2 cut(s) 196, 609
TfiI GAWTC 2 cut(s) 835, 850
Tru1I TTAA 3 cut(s) 135, 855, 903
Tru9I TTAA 3 cut(s) 135, 855, 903
TscAI CASTG 5 cut(s) 250, 316, 376, 607, 810
TseFI GTSAC 4 cut(s) 179, 215, 224, 367
TseI GCWGC 3 cut(s) 473, 719, 941
Tsp45I GTSAC 4 cut(s) 179, 215, 224, 367
TspGWI ACGGA 2 cut(s) 401, 963
TspMI CCCGGG 1 cut(s) 887
TspRI CASTG 5 cut(s) 250, 316, 376, 607, 810
VpaK11BI GGWCC 2 cut(s) 578, 673
XceI RCATGY 2 cut(s) 232, 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 887
XmiI GTMKAC 1 cut(s) 687
XspI CTAG 2 cut(s) 524, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.