Rh3CG307700

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002178
Physical Location & Seq
Reverse (-)
32183358 .. 32185617
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG307700.1

Sequence Viewer

Length: 1182 bp
ATGGAGACTTTCCTATTCACATCTGAATCTGTGAACGAGGGACACCCCGACAAGCTGTGCGACCAGATCTCTGATGCGGTGCTTGATGCTTGCCTTGAGCAGGACCCTGACAGCAAGGTTGCCTGTGAGACATGCACCAAAACAAACATGGTAATGGTGTTCGGAGAGATCACCACCAAGGCCACTGTTGACTACGAGAAGATTGTCCGTGACACATGCCGCACCATTGGGTTTGTTTCTGATGATGTCGGTCTTGATGCTGATAACTGCAAGGTCCTAGTCAACATTGAGCAGCAGAGCCCAGATATTGCCCAGGGTGTCCATGGTCACTTTACCAAGCGCCCGGAGGAGATTGGTGCTGGTGACCAGGGTCACATGTTTGGCTATGCCACTGATGAAACCCCTGAGTTGATGCCCCTTAGCCATGTCCTTGCAACCAAACTTGGTGCTCGCCTCACTGAAGTTAGAAAGAATGGTACTTGCGCATGGCTGAGACCTGATGGCAAGACCCAGGTGACAGTTGAATACCAGAATGAAGGCGGTGCTATGGTTCCACTACGTGTCCACACTGTTCTTATCTCCACCCAGCATGACGAGACTGTCACAAACGACGAGATTGCTGCTGACCTCAAGGAGCATGTTATTAAGCCTGTCGTCCCCGAGAAGTACCTGGATGAGAAGACAATCTTCCATCTTAACCCCTCTGGTCGCTTTGTGATTGGTGGCCCTCATGGTGATGCAGGTCTCACTGGACGCAAGATCATTATTGATACCTATGGTGGTTGGGGAGCCCATGGTGGTGGTGCTTTCTCTGGAAAGGACCCTACCAAGGTGGACAGGAGTGGTGCTTACATTGTCAGGCAGGCTGCCAAGAGCATTGTAGCAAATGGTCTTGCTCGCAGGGCCATCGTGCAGGTCTCCTATGCCATTGGTGTCCCTGAGCCCTTGTCGGTCTTCGTCGAAACATACGGAACTGGAAAGATCCCTGACAAGGAGATCCTTAAAATTGTGAAGGAGAACTTTGACTTCAGGCCGGGTATGATGACCATCAACCTGGACCTCAAGAGGGGTGGGAATGGAAGATTCTTGAAGACAGCCGCCTATGGGCACTTCGGAAGGGATGACCCTGACTTCACCTGGGAGGTTGTGAAGCCCCTCAAGTGGGAGAAGCCTCAGTCTTGA

Protein Analysis

393

Amino Acids

43.05

Weight (kDa)

5.51

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 3.1e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 1.9e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 240 - 381 5.2e-60 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 599
Acc16I TGCGCA 1 cut(s) 484
Acc36I ACCTGC 2 cut(s) 731, 904
AciI CCGC 4 cut(s) 77, 220, 540, 1098
AclWI GGATC 2 cut(s) 976, 991
AcuI CTGAAG 2 cut(s) 480, 1012
AdeI CACNNNGTG 1 cut(s) 560
AfaI GTAC 2 cut(s) 478, 668
AfiI CCNNNNNNNGG 7 cut(s) 100, 829, 991, 1066, 1104, 1161, 1162
AflIII ACRYGT 2 cut(s) 375, 559
AgsI TTSAA 2 cut(s) 524, 1090
AjnI CCWGG 6 cut(s) 312, 366, 510, 669, 1053, 1136
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 5 cut(s) 122, 487, 590, 749, 922
AlwI GGATC 2 cut(s) 976, 991
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 4 cut(s) 180, 724, 903, 1031
ApeKI GCWGC 3 cut(s) 292, 620, 866
AspLEI GCGC 2 cut(s) 342, 485
AspS9I GGNCC 6 cut(s) 103, 274, 725, 820, 903, 1057
AsuC2I CCSGG 2 cut(s) 344, 1035
AsuHPI GGTGA 5 cut(s) 163, 374, 526, 746, 1126
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 4 cut(s) 103, 274, 820, 1057
BaeGI GKGCMC 1 cut(s) 1110
BanII GRGCYC 3 cut(s) 302, 793, 945
BbsI GAAGAC 3 cut(s) 686, 946, 1097
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 3 cut(s) 304, 607, 853
BccI CCATC 4 cut(s) 494, 699, 914, 1055
BcgI CGANNNNNNTGC 6 cut(s) 599, 602, 633, 636, 889, 923
BciT130I CCWGG 6 cut(s) 314, 368, 512, 671, 1055, 1138
BcnI CCSGG 2 cut(s) 344, 1035
BcoDI GTCTC 5 cut(s) 122, 487, 590, 749, 922
BfaI CTAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 343
BfuAI ACCTGC 2 cut(s) 731, 904
BglII AGATCT 1 cut(s) 66
BisI GCNGC 5 cut(s) 220, 293, 621, 867, 1098
BlsI GCNGC 5 cut(s) 221, 294, 622, 868, 1099
Bme1390I CCNGG 8 cut(s) 314, 344, 368, 512, 671, 1035, 1055, 1138
Bme18I GGWCC 4 cut(s) 103, 274, 820, 1057
BmeT110I CYCGRG 1 cut(s) 659
BmgT120I GGNCC 6 cut(s) 103, 274, 725, 820, 903, 1057
BmiI GGNNCC 4 cut(s) 105, 552, 790, 822
BmrFI CCNGG 8 cut(s) 314, 344, 368, 512, 671, 1035, 1055, 1138
BmsI GCATC 5 cut(s) 64, 76, 247, 402, 727
BoxI GACNNNNGTC 1 cut(s) 369
BpiI GAAGAC 3 cut(s) 686, 946, 1097
Bpu10I CCTNAGC 2 cut(s) 419, 939
BpuEI CTTGAG 4 cut(s) 116, 614, 1046, 1142
BpuMI CCSGG 2 cut(s) 344, 1035
BsaAI YACGTR 1 cut(s) 560
BsaBI GATNNNNATC 1 cut(s) 1046
BsaI GGTCTC 3 cut(s) 487, 749, 922
BsaJI CCNNGG 9 cut(s) 177, 312, 313, 322, 367, 510, 793, 828, 1137
Bsc4I CCNNNNNNNGG 7 cut(s) 100, 829, 991, 1066, 1104, 1161, 1162
Bse1I ACTGG 2 cut(s) 754, 979
Bse8I GATNNNNATC 1 cut(s) 1046
BseBI CCWGG 6 cut(s) 314, 368, 512, 671, 1055, 1138
BseDI CCNNGG 9 cut(s) 177, 312, 313, 322, 367, 510, 793, 828, 1137
BseGI GGATG 2 cut(s) 679, 1126
BseJI GATNNNNATC 1 cut(s) 1046
BseLI CCNNNNNNNGG 7 cut(s) 100, 829, 991, 1066, 1104, 1161, 1162
BseMII CTCAG 3 cut(s) 396, 482, 930
BseNI ACTGG 2 cut(s) 754, 979
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 1110
BseXI GCAGC 3 cut(s) 304, 607, 853
BseYI CCCAGC 1 cut(s) 585
BsgI GTGCAG 1 cut(s) 932
BshFI GGCC 4 cut(s) 182, 726, 905, 1033
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 2 cut(s) 344, 1034
BslFI GGGAC 3 cut(s) 54, 641, 920
BslI CCNNNNNNNGG 7 cut(s) 100, 829, 991, 1066, 1104, 1161, 1162
BsmAI GTCTC 5 cut(s) 122, 487, 590, 749, 922
BsmFI GGGAC 3 cut(s) 54, 641, 920
BsnI GGCC 4 cut(s) 182, 726, 905, 1033
Bso31I GGTCTC 3 cut(s) 487, 749, 922
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 5 cut(s) 302, 451, 793, 945, 1110
Bsp143I GATC 5 cut(s) 66, 168, 759, 981, 996
Bsp19I CCATGG 2 cut(s) 322, 793
BspACI CCGC 4 cut(s) 77, 220, 540, 1098
BspANI GGCC 4 cut(s) 182, 726, 905, 1033
BspCNI CTCAG 3 cut(s) 397, 483, 931
BspLI GGNNCC 4 cut(s) 105, 552, 790, 822
BspMI ACCTGC 2 cut(s) 731, 904
BspPI GGATC 2 cut(s) 976, 991
BspTNI GGTCTC 3 cut(s) 487, 749, 922
BsrI ACTGG 2 cut(s) 754, 979
BssECI CCNNGG 9 cut(s) 177, 312, 313, 322, 367, 510, 793, 828, 1137
BssMI GATC 5 cut(s) 66, 168, 759, 981, 996
BssT1I CCWWGG 4 cut(s) 177, 322, 793, 828
Bst2UI CCWGG 6 cut(s) 314, 368, 512, 671, 1055, 1138
Bst4CI ACNGT 4 cut(s) 187, 520, 571, 601
BstBAI YACGTR 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 91, 451, 864, 898
BstDEI CTNAG 5 cut(s) 405, 419, 491, 939, 1173
BstDSI CCRYGG 2 cut(s) 322, 793
BstEII GGTNACC 1 cut(s) 362
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 679, 1126
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 2 cut(s) 342, 485
BstKTI GATC 5 cut(s) 69, 171, 762, 984, 999
BstMAI GTCTC 5 cut(s) 122, 487, 590, 749, 922
BstMBI GATC 5 cut(s) 66, 168, 759, 981, 996
BstMWI GCNNNNNNNGC 1 cut(s) 902
BstNI CCWGG 6 cut(s) 314, 368, 512, 671, 1055, 1138
BstNSI RCATGY 4 cut(s) 135, 219, 379, 641
BstPAI GACNNNNGTC 1 cut(s) 369
BstPI GGTNACC 1 cut(s) 362
BstSCI CCNGG 8 cut(s) 312, 342, 366, 510, 669, 1033, 1053, 1136
BstSLI GKGCMC 1 cut(s) 1110
BstV1I GCAGC 3 cut(s) 304, 607, 853
BstV2I GAAGAC 3 cut(s) 686, 946, 1097
BstX2I RGATCY 3 cut(s) 66, 981, 996
BstXI CCANNNNNNTGG 2 cut(s) 800, 1054
BstYI RGATCY 3 cut(s) 66, 981, 996
BsuRI GGCC 4 cut(s) 182, 726, 905, 1033
BtgI CCRYGG 2 cut(s) 322, 793
BtsCI GGATG 2 cut(s) 679, 1126
BtsIMutI CAGTG 5 cut(s) 183, 390, 456, 567, 747
BveI ACCTGC 2 cut(s) 731, 904
Cac8I GCNNGC 4 cut(s) 91, 451, 864, 898
CfoI GCGC 2 cut(s) 342, 485
Cfr13I GGNCC 6 cut(s) 103, 274, 725, 820, 903, 1057
CseI GACGC 1 cut(s) 762
Csp6I GTAC 2 cut(s) 477, 667
CspCI CAANNNNNGTGG 2 cut(s) 1051, 1086
CviQI GTAC 2 cut(s) 477, 667
DdeI CTNAG 5 cut(s) 405, 419, 491, 939, 1173
DpnI GATC 5 cut(s) 68, 170, 761, 983, 998
DpnII GATC 5 cut(s) 66, 168, 759, 981, 996
DraIII CACNNNGTG 1 cut(s) 560
DrdI GACNNNNNNGTC 1 cut(s) 599
DseDI GACNNNNNNGTC 1 cut(s) 599
Eco130I CCWWGG 4 cut(s) 177, 322, 793, 828
Eco24I GRGCYC 3 cut(s) 302, 793, 945
Eco31I GGTCTC 3 cut(s) 487, 749, 922
Eco47I GGWCC 4 cut(s) 103, 274, 820, 1057
Eco57I CTGAAG 2 cut(s) 480, 1012
Eco88I CYCGRG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 362
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO109I RGGNCCY 3 cut(s) 103, 274, 820
EcoO65I GGTNACC 1 cut(s) 362
EcoRII CCWGG 6 cut(s) 312, 366, 510, 669, 1053, 1136
EcoT14I CCWWGG 4 cut(s) 177, 322, 793, 828
EcoT38I GRGCYC 3 cut(s) 302, 793, 945
ErhI CCWWGG 4 cut(s) 177, 322, 793, 828
FalI AAGNNNNNCTT 4 cut(s) 671, 703, 1004, 1036
FaqI GGGAC 3 cut(s) 54, 641, 920
Fnu4HI GCNGC 5 cut(s) 220, 293, 621, 867, 1098
FokI GGATG 2 cut(s) 686, 1133
FriOI GRGCYC 3 cut(s) 302, 793, 945
Fsp4HI GCNGC 5 cut(s) 220, 293, 621, 867, 1098
FspBI CTAG 1 cut(s) 278
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 2 cut(s) 341, 484
GluI GCNGC 5 cut(s) 220, 293, 621, 867, 1098
GsaI CCCAGC 1 cut(s) 589
HaeII RGCGCY 1 cut(s) 343
HaeIII GGCC 4 cut(s) 182, 726, 905, 1033
HapII CCGG 2 cut(s) 344, 1034
HgaI GACGC 1 cut(s) 762
HhaI GCGC 2 cut(s) 342, 485
Hin6I GCGC 2 cut(s) 340, 483
HinP1I GCGC 2 cut(s) 340, 483
HincII GTYRAC 2 cut(s) 190, 283
HindII GTYRAC 2 cut(s) 190, 283
HinfI GANTC 2 cut(s) 26, 1083
HpaII CCGG 2 cut(s) 344, 1034
HphI GGTGA 5 cut(s) 163, 374, 526, 746, 1126
Hpy166II GTNNAC 5 cut(s) 34, 190, 283, 565, 835
Hpy188I TCNGA 5 cut(s) 25, 73, 164, 241, 1115
Hpy188III TCNNGA 5 cut(s) 254, 813, 1063, 1087, 1179
Hpy8I GTNNAC 5 cut(s) 34, 190, 283, 565, 835
Hpy99I CGWCG 2 cut(s) 614, 962
HpyAV CCTTC 3 cut(s) 530, 1006, 1110
HpyCH4III ACNGT 4 cut(s) 187, 520, 571, 601
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 5 cut(s) 135, 270, 434, 740, 913
HpyF10VI GCNNNNNNNGC 1 cut(s) 902
HpyF3I CTNAG 5 cut(s) 405, 419, 491, 939, 1173
HpySE526I ACGT 1 cut(s) 559
HspAI GCGC 2 cut(s) 340, 483
Kzo9I GATC 5 cut(s) 66, 168, 759, 981, 996
LmnI GCTCC 2 cut(s) 634, 788
Lsp1109I GCAGC 3 cut(s) 304, 607, 853
LweI GCATC 5 cut(s) 64, 76, 247, 402, 727
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 6 cut(s) 209, 326, 362, 371, 514, 601
MalI GATC 5 cut(s) 68, 170, 761, 983, 998
MboI GATC 5 cut(s) 66, 168, 759, 981, 996
MboII GAAGA 6 cut(s) 211, 679, 691, 946, 1092, 1102
MflI RGATCY 3 cut(s) 66, 981, 996
MhlI GDGCHC 5 cut(s) 302, 451, 793, 945, 1110
MluCI AATT 1 cut(s) 1005
MseI TTAA 3 cut(s) 645, 696, 1002
MslI CAYNNNNRTG 3 cut(s) 152, 735, 798
MspI CCGG 2 cut(s) 344, 1034
MspR9I CCNGG 8 cut(s) 314, 344, 368, 512, 671, 1035, 1055, 1138
MvaI CCWGG 6 cut(s) 314, 368, 512, 671, 1055, 1138
MwoI GCNNNNNNNGC 1 cut(s) 902
NciI CCSGG 2 cut(s) 344, 1035
NcoI CCATGG 2 cut(s) 322, 793
NdeII GATC 5 cut(s) 66, 168, 759, 981, 996
NlaIV GGNNCC 4 cut(s) 105, 552, 790, 822
NmuCI GTSAC 6 cut(s) 209, 326, 362, 371, 514, 601
NsbI TGCGCA 1 cut(s) 484
NspI RCATGY 4 cut(s) 135, 219, 379, 641
PasI CCCWGGG 1 cut(s) 313
PciI ACATGT 1 cut(s) 375
PfeI GAWTC 2 cut(s) 26, 1083
PkrI GCNGC 5 cut(s) 221, 294, 622, 868, 1099
Ppu21I YACGTR 1 cut(s) 560
PpuMI RGGWCCY 3 cut(s) 103, 274, 820
PscI ACATGT 1 cut(s) 375
PshAI GACNNNNGTC 1 cut(s) 369
Psp5II RGGWCCY 3 cut(s) 103, 274, 820
Psp6I CCWGG 6 cut(s) 312, 366, 510, 669, 1053, 1136
PspEI GGTNACC 1 cut(s) 362
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 6 cut(s) 312, 366, 510, 669, 1053, 1136
PspN4I GGNNCC 4 cut(s) 105, 552, 790, 822
PspPI GGNCC 6 cut(s) 103, 274, 725, 820, 903, 1057
PspPPI RGGWCCY 3 cut(s) 103, 274, 820
PsuI RGATCY 3 cut(s) 66, 981, 996
RsaI GTAC 2 cut(s) 478, 668
RsaNI GTAC 2 cut(s) 477, 667
RseI CAYNNNNRTG 3 cut(s) 152, 735, 798
SaqAI TTAA 3 cut(s) 645, 696, 1002
SatI GCNGC 5 cut(s) 220, 293, 621, 867, 1098
Sau3AI GATC 5 cut(s) 66, 168, 759, 981, 996
Sau96I GGNCC 6 cut(s) 103, 274, 725, 820, 903, 1057
ScrFI CCNGG 8 cut(s) 314, 344, 368, 512, 671, 1035, 1055, 1138
SduI GDGCHC 5 cut(s) 302, 451, 793, 945, 1110
SfaNI GCATC 5 cut(s) 64, 76, 247, 402, 727
SinI GGWCC 4 cut(s) 103, 274, 820, 1057
SmiMI CAYNNNNRTG 3 cut(s) 152, 735, 798
SmlI CTYRAG 4 cut(s) 95, 629, 1061, 1157
SmoI CTYRAG 4 cut(s) 95, 629, 1061, 1157
Sse9I AATT 1 cut(s) 1005
SsiI CCGC 4 cut(s) 77, 220, 540, 1098
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 8 cut(s) 312, 342, 366, 510, 669, 1033, 1053, 1136
StyI CCWWGG 4 cut(s) 177, 322, 793, 828
TaaI ACNGT 4 cut(s) 187, 520, 571, 601
TaiI ACGT 1 cut(s) 562
TaqI TCGA 1 cut(s) 960
TaqII GACCGA 2 cut(s) 239, 940
TasI AATT 1 cut(s) 1005
TauI GCSGC 2 cut(s) 222, 1100
TfiI GAWTC 2 cut(s) 26, 1083
Tru1I TTAA 3 cut(s) 645, 696, 1002
Tru9I TTAA 3 cut(s) 645, 696, 1002
TscAI CASTG 5 cut(s) 190, 397, 463, 574, 754
TseFI GTSAC 6 cut(s) 209, 326, 362, 371, 514, 601
TseI GCWGC 3 cut(s) 292, 620, 866
Tsp45I GTSAC 6 cut(s) 209, 326, 362, 371, 514, 601
TspDTI ATGAA 2 cut(s) 411, 549
TspGWI ACGGA 2 cut(s) 197, 984
TspRI CASTG 5 cut(s) 190, 397, 463, 574, 754
VpaK11BI GGWCC 4 cut(s) 103, 274, 820, 1057
XagI CCTNNNNNAGG 1 cut(s) 98
XceI RCATGY 4 cut(s) 135, 219, 379, 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 320
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.