Rw3G007340

Catalyzes the formation of S-adenosylmethionine from methionine and ATP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002178
Physical Location & Seq
Forward (+)
6785032 .. 6787711
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007340.1

Sequence Viewer

Length: 1119 bp
ATGGACACCTTCCTCTTCACCTCCGAGTCTGTGAATGAGGGACACCCCGATAAACTCTGTGATCAGATTTCCGATGCAGTGCTTGATGCCTGCCTTGAACAAGATCCTGACAGCAAAGTTGCCTGTGAGACCTGCACCAAGACCAACATGGTCATGGTCTTCGGAGAGATCACAACTAAAGCCGAAGTGGACTATGAGAAGATAGTGCGCAACACCTGCCGCAGCATTGGATTTGTTTCAGGTGATGTAGGCCTTGATGCTGATAACTGCAAGGTCCTCGTGAACATTGAGCAACAAAGCCCTGATATAGCTCAGGGTGTGCATGGCCACCTCACCAAGAGGCCTGAGGAGATTGGTGCAGGAGACCAGGGTCACATGTTTGGTTATGCTACTGATGAGACCCCTGAGCTTATGCCTCTAAGCCATGTTCTTGCTACCAAGCTTGGTGCTCGTCTCACTGATGTTCGAAAGAATGGCACCTGCCCTTGGCTGAGACCTGATGGCAAGACTCAAGTCACTGTTGAGTACTACAATGACCATGGTGCTATGGTTCCAATCCGTGTCCACACTGTTCTCATTTCTACCCAGCATGATGAGACTGTTACCAATGATGAAATTGCAGCTGACCTCAAGAAACATGTCATTAAGCCAGTTGTTCCTGAGAAGTACCTAGATGAGAAGACTATCTTCCATCTCAACCCTTCAGGTCGATTTGTGATTGGTGGACCGCATGGTGATGCAGCACATGGCGGTGGTGCCTTCTCCGGCAAGGACCCTACCAAGGTAGACAGGAGTGGTGCCTACATTGTTAGGCAGGCTGCTAAGAGCATTGTAGCCAATGGACTTGCTAGAAGGTGCATAGTGCAGGTCTCATATGCAATTGGTGTCCCTGAGCCACTATCGGTGTTTGTTGACACTTATGGGACTGGAAAGATTCCGGACAAGGAGATTCTGAAGTTAGTTAAGGAGGCCTTTGATTTTAGGCCTGGAATGATTGCCATTAACTTGGATCTCAAGAGAGGAAGGTATTTGAAAACGGCTGCTTATGGACACTTTGGTAGAGATGACCCTGACTTTTCGTGGGAAGTGGTGAAGTCCTTGAAGTGGGAAAAGGCGTAA

Protein Analysis

372

Amino Acids

40.82

Weight (kDa)

5.73

Isoelectric Point (pI)

26.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-AdoMet_synt_N PF00438 4 - 101 3.8e-43 S-adenosylmethionine synthetase, N-terminal domain
S-AdoMet_synt_M PF02772 117 - 238 3e-47 S-adenosylmethionine synthetase, central domain
S-AdoMet_synt_C PF02773 245 - 362 3.3e-47 S-adenosylmethionine synthetase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 224, 488
Acc16I TGCGCA 1 cut(s) 209
Acc36I ACCTGC 4 cut(s) 140, 224, 488, 856
AccB1I GGYRCC 3 cut(s) 476, 755, 797
AccI GTMKAC 1 cut(s) 786
AccIII TCCGGA 1 cut(s) 937
AciI CCGC 3 cut(s) 220, 728, 750
AclWI GGATC 2 cut(s) 98, 1017
AcoI YGGCCR 1 cut(s) 325
AcuI CTGAAG 2 cut(s) 687, 974
AfaI GTAC 2 cut(s) 527, 668
AfiI CCNNNNNNNGG 3 cut(s) 486, 781, 1104
AflIII ACRYGT 2 cut(s) 375, 637
AgsI TTSAA 3 cut(s) 98, 1033, 1102
AjnI CCWGG 2 cut(s) 366, 985
AluBI AGCT 4 cut(s) 311, 409, 442, 623
AluI AGCT 4 cut(s) 311, 409, 442, 623
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 874
AlwI GGATC 2 cut(s) 98, 1017
Aor13HI TCCGGA 1 cut(s) 937
AoxI GGCC 5 cut(s) 250, 325, 341, 969, 983
ApeKI GCWGC 5 cut(s) 222, 620, 740, 818, 1040
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 3 cut(s) 274, 725, 772
AsuHPI GGTGA 5 cut(s) 10, 254, 325, 746, 1102
AsuII TTCGAA 1 cut(s) 466
AvaII GGWCC 3 cut(s) 274, 725, 772
AxyI CCTNAGG 1 cut(s) 345
BalI TGGCCA 1 cut(s) 327
BanI GGYRCC 3 cut(s) 476, 755, 797
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 2 cut(s) 151, 686
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 5 cut(s) 234, 632, 752, 805, 1027
BccI CCATC 2 cut(s) 494, 699
BceAI ACGGC 1 cut(s) 1053
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 2 cut(s) 368, 987
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 874
BfaI CTAG 2 cut(s) 671, 849
BfuAI ACCTGC 4 cut(s) 140, 224, 488, 856
BisI GCNGC 6 cut(s) 220, 223, 621, 741, 819, 1041
BlsI GCNGC 6 cut(s) 221, 224, 622, 742, 820, 1042
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 368, 987
Bme18I GGWCC 3 cut(s) 274, 725, 772
BmgT120I GGNCC 3 cut(s) 274, 725, 772
BmiI GGNNCC 5 cut(s) 478, 552, 757, 774, 799
BmrFI CCNGG 2 cut(s) 368, 987
BmsI GCATC 4 cut(s) 64, 76, 247, 727
BoxI GACNNNNGTC 2 cut(s) 369, 512
BpiI GAAGAC 2 cut(s) 151, 686
Bpu10I CCTNAGC 3 cut(s) 312, 405, 891
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 3 cut(s) 495, 614, 998
BsaI GGTCTC 5 cut(s) 122, 357, 392, 487, 874
BsaJI CCNNGG 4 cut(s) 367, 485, 538, 780
BsaWI WCCGGW 1 cut(s) 937
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 781, 1104
Bse1I ACTGG 2 cut(s) 650, 931
Bse21I CCTNAGG 1 cut(s) 345
BseAI TCCGGA 1 cut(s) 937
BseBI CCWGG 2 cut(s) 368, 987
BseDI CCNNGG 4 cut(s) 367, 485, 538, 780
BseLI CCNNNNNNNGG 3 cut(s) 486, 781, 1104
BseMII CTCAG 6 cut(s) 326, 336, 396, 482, 651, 882
BseNI ACTGG 2 cut(s) 650, 931
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 5 cut(s) 234, 632, 752, 805, 1027
BseYI CCCAGC 1 cut(s) 585
BsgI GTGCAG 3 cut(s) 118, 378, 884
BshFI GGCC 5 cut(s) 252, 327, 343, 971, 985
BshNI GGYRCC 3 cut(s) 476, 755, 797
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 2 cut(s) 765, 938
BslFI GGGAC 3 cut(s) 54, 872, 937
BslI CCNNNNNNNGG 3 cut(s) 486, 781, 1104
BsmAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 874
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 3 cut(s) 54, 872, 937
BsnI GGCC 5 cut(s) 252, 327, 343, 971, 985
Bso31I GGTCTC 5 cut(s) 122, 357, 392, 487, 874
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 1 cut(s) 451
Bsp13I TCCGGA 1 cut(s) 937
Bsp143I GATC 4 cut(s) 61, 103, 168, 1009
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 3 cut(s) 220, 728, 750
BspANI GGCC 5 cut(s) 252, 327, 343, 971, 985
BspCNI CTCAG 6 cut(s) 325, 337, 397, 483, 652, 883
BspEI TCCGGA 1 cut(s) 937
BspLI GGNNCC 5 cut(s) 478, 552, 757, 774, 799
BspMI ACCTGC 4 cut(s) 140, 224, 488, 856
BspPI GGATC 2 cut(s) 98, 1017
BspT104I TTCGAA 1 cut(s) 466
BspT107I GGYRCC 3 cut(s) 476, 755, 797
BspTNI GGTCTC 5 cut(s) 122, 357, 392, 487, 874
BsrI ACTGG 2 cut(s) 650, 931
BssECI CCNNGG 4 cut(s) 367, 485, 538, 780
BssMI GATC 4 cut(s) 61, 103, 168, 1009
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 3 cut(s) 485, 538, 780
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 368, 987
Bst4CI ACNGT 3 cut(s) 520, 571, 601
Bst6I CTCTTC 1 cut(s) 20
BstAPI GCANNNNNTGC 1 cut(s) 216
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 2 cut(s) 91, 816
BstDEI CTNAG 8 cut(s) 312, 345, 405, 419, 491, 660, 822, 891
BstDSI CCRYGG 1 cut(s) 538
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 4 cut(s) 64, 106, 171, 1012
BstMAI GTCTC 7 cut(s) 122, 357, 392, 458, 487, 590, 874
BstMBI GATC 4 cut(s) 61, 103, 168, 1009
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 2 cut(s) 368, 987
BstNSI RCATGY 2 cut(s) 379, 641
BstPAI GACNNNNGTC 2 cut(s) 369, 512
BstSCI CCNGG 2 cut(s) 366, 985
BstV1I GCAGC 5 cut(s) 234, 632, 752, 805, 1027
BstV2I GAAGAC 2 cut(s) 151, 686
BstX2I RGATCY 2 cut(s) 103, 1009
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstYI RGATCY 2 cut(s) 103, 1009
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 5 cut(s) 252, 327, 343, 971, 985
BtgI CCRYGG 1 cut(s) 538
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 4 cut(s) 84, 456, 516, 567
BveI ACCTGC 4 cut(s) 140, 224, 488, 856
Cac8I GCNNGC 2 cut(s) 91, 816
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 3 cut(s) 274, 725, 772
Csp6I GTAC 2 cut(s) 526, 667
CviQI GTAC 2 cut(s) 526, 667
DdeI CTNAG 8 cut(s) 312, 345, 405, 419, 491, 660, 822, 891
DpnI GATC 4 cut(s) 63, 105, 170, 1011
DpnII GATC 4 cut(s) 61, 103, 168, 1009
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 485, 538, 780
Eco147I AGGCCT 4 cut(s) 252, 343, 971, 985
Eco31I GGTCTC 5 cut(s) 122, 357, 392, 487, 874
Eco47I GGWCC 3 cut(s) 274, 725, 772
Eco57I CTGAAG 2 cut(s) 687, 974
Eco81I CCTNAGG 1 cut(s) 345
EcoO109I RGGNCCY 2 cut(s) 274, 772
EcoRII CCWGG 2 cut(s) 366, 985
EcoT14I CCWWGG 3 cut(s) 485, 538, 780
ErhI CCWWGG 3 cut(s) 485, 538, 780
Esp3I CGTCTC 1 cut(s) 458
FalI AAGNNNNNCTT 4 cut(s) 671, 703, 956, 988
FaqI GGGAC 3 cut(s) 54, 872, 937
FauNDI CATATG 1 cut(s) 874
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 6 cut(s) 220, 223, 621, 741, 819, 1041
Fsp4HI GCNGC 6 cut(s) 220, 223, 621, 741, 819, 1041
FspBI CTAG 2 cut(s) 671, 849
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 1 cut(s) 209
GluI GCNGC 6 cut(s) 220, 223, 621, 741, 819, 1041
GsaI CCCAGC 1 cut(s) 589
HaeIII GGCC 5 cut(s) 252, 327, 343, 971, 985
HapII CCGG 2 cut(s) 765, 938
HhaI GCGC 1 cut(s) 210
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 4 cut(s) 26, 508, 934, 949
HpaII CCGG 2 cut(s) 765, 938
HphI GGTGA 5 cut(s) 10, 254, 325, 746, 1102
Hpy166II GTNNAC 6 cut(s) 190, 283, 565, 725, 787, 913
Hpy188I TCNGA 5 cut(s) 25, 66, 73, 164, 954
Hpy188III TCNNGA 6 cut(s) 107, 280, 631, 659, 938, 1015
Hpy8I GTNNAC 6 cut(s) 190, 283, 565, 725, 787, 913
HpyAV CCTTC 5 cut(s) 19, 711, 769, 846, 1017
HpyCH4III ACNGT 3 cut(s) 520, 571, 601
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
HpyF3I CTNAG 8 cut(s) 312, 345, 405, 419, 491, 660, 822, 891
HspAI GCGC 1 cut(s) 208
Kpn2I TCCGGA 1 cut(s) 937
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 4 cut(s) 61, 103, 168, 1009
Lsp1109I GCAGC 5 cut(s) 234, 632, 752, 805, 1027
LweI GCATC 4 cut(s) 64, 76, 247, 727
MaeI CTAG 2 cut(s) 671, 849
MaeIII GTNAC 3 cut(s) 371, 514, 601
MalI GATC 4 cut(s) 63, 105, 170, 1011
MboI GATC 4 cut(s) 61, 103, 168, 1009
MboII GAAGA 5 cut(s) 7, 151, 211, 679, 691
MfeI CAATTG 1 cut(s) 879
MflI RGATCY 2 cut(s) 103, 1009
MhlI GDGCHC 1 cut(s) 451
MlsI TGGCCA 1 cut(s) 327
MluCI AATT 2 cut(s) 615, 879
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 2 cut(s) 35, 502
Mox20I TGGCCA 1 cut(s) 327
MroI TCCGGA 1 cut(s) 937
MscI TGGCCA 1 cut(s) 327
MseI TTAA 3 cut(s) 645, 963, 1002
MslI CAYNNNNRTG 3 cut(s) 152, 735, 750
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 2 cut(s) 765, 938
MspR9I CCNGG 2 cut(s) 368, 987
MunI CAATTG 1 cut(s) 879
MvaI CCWGG 2 cut(s) 368, 987
MwoI GCNNNNNNNGC 1 cut(s) 216
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 1 cut(s) 874
NdeII GATC 4 cut(s) 61, 103, 168, 1009
NlaIV GGNNCC 5 cut(s) 478, 552, 757, 774, 799
NmuCI GTSAC 2 cut(s) 371, 514
NsbI TGCGCA 1 cut(s) 209
NspI RCATGY 2 cut(s) 379, 641
NspV TTCGAA 1 cut(s) 466
PaqCI CACCTGC 2 cut(s) 224, 488
PceI AGGCCT 4 cut(s) 252, 343, 971, 985
PciI ACATGT 2 cut(s) 375, 637
PfeI GAWTC 2 cut(s) 934, 949
PkrI GCNGC 6 cut(s) 221, 224, 622, 742, 820, 1042
PleI GAGTC 2 cut(s) 34, 502
PpsI GAGTC 2 cut(s) 34, 502
PpuMI RGGWCCY 2 cut(s) 274, 772
PscI ACATGT 2 cut(s) 375, 637
PshAI GACNNNNGTC 2 cut(s) 369, 512
Psp5II RGGWCCY 2 cut(s) 274, 772
Psp6I CCWGG 2 cut(s) 366, 985
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 2 cut(s) 366, 985
PspN4I GGNNCC 5 cut(s) 478, 552, 757, 774, 799
PspPI GGNCC 3 cut(s) 274, 725, 772
PspPPI RGGWCCY 2 cut(s) 274, 772
PsuI RGATCY 2 cut(s) 103, 1009
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 527, 668
RsaNI GTAC 2 cut(s) 526, 667
RseI CAYNNNNRTG 3 cut(s) 152, 735, 750
SaqAI TTAA 3 cut(s) 645, 963, 1002
SatI GCNGC 6 cut(s) 220, 223, 621, 741, 819, 1041
Sau3AI GATC 4 cut(s) 61, 103, 168, 1009
Sau96I GGNCC 3 cut(s) 274, 725, 772
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 35, 502
ScrFI CCNGG 2 cut(s) 368, 987
SduI GDGCHC 1 cut(s) 451
SfaNI GCATC 4 cut(s) 64, 76, 247, 727
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 3 cut(s) 274, 725, 772
SmiMI CAYNNNNRTG 3 cut(s) 152, 735, 750
SmlI CTYRAG 3 cut(s) 510, 629, 1013
SmoI CTYRAG 3 cut(s) 510, 629, 1013
Sse9I AATT 2 cut(s) 615, 879
SseBI AGGCCT 4 cut(s) 252, 343, 971, 985
SsiI CCGC 3 cut(s) 220, 728, 750
SspMI CTAG 2 cut(s) 671, 849
StuI AGGCCT 4 cut(s) 252, 343, 971, 985
StyD4I CCNGG 2 cut(s) 366, 985
StyI CCWWGG 3 cut(s) 485, 538, 780
TaaI ACNGT 3 cut(s) 520, 571, 601
TaqI TCGA 2 cut(s) 466, 709
TasI AATT 2 cut(s) 615, 879
TatI WGTACW 1 cut(s) 525
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 2 cut(s) 934, 949
Tru1I TTAA 3 cut(s) 645, 963, 1002
Tru9I TTAA 3 cut(s) 645, 963, 1002
TscAI CASTG 4 cut(s) 84, 463, 523, 574
TseFI GTSAC 2 cut(s) 371, 514
TseI GCWGC 5 cut(s) 222, 620, 740, 818, 1040
Tsp45I GTSAC 2 cut(s) 371, 514
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 4 cut(s) 84, 463, 523, 574
VpaK11BI GGWCC 3 cut(s) 274, 725, 772
XceI RCATGY 2 cut(s) 379, 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 145, 151
XmiI GTMKAC 1 cut(s) 786
XspI CTAG 2 cut(s) 671, 849
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.