Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0002070

Physical Location & Seq

Genomic Coordinates Forward (+)

6498499 .. 6499607

Gene Structure 1109 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g10830.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGCTATTCCTACTTCAGACTTCCTTCAGATGAATACTAGGATACCTGAGAACCCATTTCATCATCTTCCTTTTGCATCACAAAGAGCATTAACCTTTTCAGTCAAGAAACTAGCTTGGCCAAATCCAAGGGCGGCTCTCACCCTCACTCGTGCCCCTAGTCCTACCATAAACATCTCACAAACTTTCACAAAATTGAAATCACAAGGGAAAGTGGCCTTCATTCCCTACATCACTGCCGGCGATCCCGATCTCACGACAACCATTCAAGCCCTGAAGGTGCTCGATTCTTGTGGATCCGATGTCATCGAGCTAGGCATACCGTGCTCCGATCCCTCACTAGACGGTCCGGTCATACGTGCTTCAGCGTCACGTTCCTTGGCAGGAGGGACAAAGCTCAACAATATCATCTCAATGTTAGAAGATGTTATTCCCCAGCTGTCGTGCCCTATCACTCTATTATCCTACTCTAAACCTATTATCAGATATGGCATCGGAAACTTCATGTCTACCATTAGCCAAATAGGAGTCCGTGGGCTTGTGGTTCCGGATGTGCCTTTTGAAAACACACAAAGTTTGAGAAATGAAGCGGCGAAGAACAACGTTGAGTTGGTGCTGCTAACTACACCTACTACTACAACATCTCAAATGAAAAACATTGTCCGAGCCTCGGAAGGTTTTGTTTACCTGGTGAGTGCGCTGGGGGTCACCGGAGCTCGTCCGCGTGTGAACCAACAAGTTCCGAATCTCTTGAGGGAGATTAAGGAGGTGACAAGCAAGGCGGTGGCGGTTGGGTTCGGGTTGTCGAAGCCGGAGCATGTGAAGCAGGTGGCGGAGTGGGGAGCGGATGGTGCGATTGTCGGTAGTGCTATTGTGAAGGTACTGGGCGAAGCTAGATCTCCATTGCAAGGGTTGAGAGACCTTGAATCTTTCGCCAAATCTTTCAAATCTGCTCTCCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000162 GO:0003674 GO:0003824 GO:0004834 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006568 GO:0006576 GO:0006586 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009072 GO:0009073 GO:0009308 GO:0009309 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009606 GO:0009607 GO:0009617 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0016043 GO:0016053 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0019752 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042401 GO:0042430 GO:0042435 GO:0042545 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044106 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045229 GO:0046219 GO:0046394 GO:0046483 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0098542 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00260 ko00400 ko00402 ko01100 ko01110 ko01130 ko01230 map00260 map00400 map00402 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

Trp_syntA

Protein Analysis

321

Amino Acids

34.32

Weight (kDa)

9.46

Isoelectric Point (pI)

35.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trp_syntA	PF00290	63 - 318	6.7e-87	Tryptophan synthase alpha chain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02610
fragaria_vesca	FvH4_6g10820 FvH4_6g10830
malus_domestica	MD17G1246800.v1.1
prunus_persica	Prupe.3G144200_v2.0.a1 Prupe.3G144300_v2.0.a1 Prupe.3G144400_v2.0.a1 Prupe.3G144500_v2.0.a1
pyrus_communis	pycom17g24810
rosa_chinensis	RchiOBHm_Chr1g0317261 RchiOBHm_Chr1g0325481 RchiOBHm_Chr3g0462061 RchiOBHm_Chr3g0462091 RchiOBHm_Chr5g0067051
rosa_laevigata	RLG00000013482 RLG00000024883 RLG00000030122
rosa_multiflora	Rmu_sc0007627.1_g000011 Rmu_sc0009268.1_g000017
rosa_roxburghii	Rroxscaffold_1G00014060 Rroxscaffold_6G00417740 Rroxscaffold_6G00417750
rosa_rugosa	Rorug03G0054600
rosa_samantha	Rh1AG067300 Rh1BG055800 Rh1CG068700 Rh1DG073600 Rh3AG113000 Rh3AG113100 Rh3BG116000 Rh3CG118700 Rh3CG118800 Rh3DG117800 Rh3DG117900 Rh5AG440900 Rh5BG458200 Rh5CG480000 Rh5DG473200
rosa_wichuraiana	Rw0G007850 Rw0G020470 Rw3G009530 Rw3G009540 Rw4G030480 Rw5G041210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	817
AasI	GACNNNNNNGTC	1 cut(s)	350
Acc36I	ACCTGC	1 cut(s)	817
AccBSI	CCGCTC	1 cut(s)	845
AccI	GTMKAC	1 cut(s)	509
AccII	CGCG	1 cut(s)	724
AccIII	TCCGGA	1 cut(s)	547
AciI	CCGC	7 cut(s)	134, 590, 722, 782, 788, 833, 845
AclI	AACGTT	1 cut(s)	603
AclWI	GGATC	4 cut(s)	239, 291, 304, 326
AcoI	YGGCCR	1 cut(s)	119
AcuI	CTGAAG	3 cut(s)	11, 296, 348
AfaI	GTAC	1 cut(s)	882
AfiI	CCNNNNNNNGG	2 cut(s)	670, 908
AgsI	TTSAA	5 cut(s)	199, 269, 563, 926, 946
AjnI	CCWGG	1 cut(s)	687
AluBI	AGCT	6 cut(s)	116, 313, 397, 439, 716, 893
AluI	AGCT	6 cut(s)	116, 313, 397, 439, 716, 893
Alw21I	GWGCWC	3 cut(s)	285, 329, 718
Alw26I	GTCTC	1 cut(s)	912
AlwI	GGATC	4 cut(s)	239, 291, 304, 326
Aor13HI	TCCGGA	1 cut(s)	547
AoxI	GGCC	2 cut(s)	119, 216
ApeKI	GCWGC	1 cut(s)	616
AspLEI	GCGC	1 cut(s)	700
AspS9I	GGNCC	1 cut(s)	347
AsuHPI	GGTGA	4 cut(s)	133, 700, 703, 781
AvaII	GGWCC	1 cut(s)	347
BaeGI	GKGCMC	2 cut(s)	157, 449
BalI	TGGCCA	1 cut(s)	121
BamHI	GGATCC	1 cut(s)	296
BanII	GRGCYC	1 cut(s)	718
BauI	CACGAG	1 cut(s)	150
Bbv12I	GWGCWC	3 cut(s)	285, 329, 718
BbvI	GCAGC	1 cut(s)	603
BccI	CCATC	1 cut(s)	842
BciT130I	CCWGG	1 cut(s)	689
BciVI	GTATCC	1 cut(s)	36
BcoDI	GTCTC	1 cut(s)	912
BfaI	CTAG	6 cut(s)	39, 113, 159, 314, 341, 894
BfuAI	ACCTGC	1 cut(s)	817
BfuI	GTATCC	1 cut(s)	36
BglII	AGATCT	1 cut(s)	896
BisI	GCNGC	3 cut(s)	135, 591, 617
BlsI	GCNGC	3 cut(s)	136, 592, 618
Bme1390I	CCNGG	1 cut(s)	689
Bme18I	GGWCC	1 cut(s)	347
BmgT120I	GGNCC	1 cut(s)	347
BmiI	GGNNCC	2 cut(s)	298, 546
BmrFI	CCNGG	1 cut(s)	689
BmrI	ACTGGG	1 cut(s)	893
BmsI	GCATC	2 cut(s)	86, 501
BmuI	ACTGGG	1 cut(s)	893
BpuEI	CTTGAG	1 cut(s)	772
BsaAI	YACGTR	1 cut(s)	359
BsaBI	GATNNNNATC	1 cut(s)	249
BsaI	GGTCTC	1 cut(s)	912
BsaJI	CCNNGG	4 cut(s)	128, 378, 532, 669
BsaWI	WCCGGW	3 cut(s)	349, 547, 710
Bsc4I	CCNNNNNNNGG	2 cut(s)	670, 908
Bse118I	RCCGGY	1 cut(s)	239
Bse1I	ACTGG	1 cut(s)	888
Bse3DI	GCAATG	1 cut(s)	902
Bse8I	GATNNNNATC	1 cut(s)	249
BseAI	TCCGGA	1 cut(s)	547
BseBI	CCWGG	1 cut(s)	689
BseDI	CCNNGG	4 cut(s)	128, 378, 532, 669
BseGI	GGATG	2 cut(s)	556, 853
BseJI	GATNNNNATC	1 cut(s)	249
BseLI	CCNNNNNNNGG	2 cut(s)	670, 908
BseMI	GCAATG	1 cut(s)	902
BseMII	CTCAG	1 cut(s)	39
BseNI	ACTGG	1 cut(s)	888
BseSI	GKGCMC	2 cut(s)	157, 449
BseXI	GCAGC	1 cut(s)	603
BseYI	CCCAGC	2 cut(s)	435, 700
Bsh1236I	CGCG	1 cut(s)	724
BshFI	GGCC	2 cut(s)	121, 218
BsiHKAI	GWGCWC	3 cut(s)	285, 329, 718
BsiSI	CCGG	5 cut(s)	240, 350, 548, 711, 812
BslFI	GGGAC	1 cut(s)	403
BslI	CCNNNNNNNGG	2 cut(s)	670, 908
BsmAI	GTCTC	1 cut(s)	912
BsmFI	GGGAC	1 cut(s)	403
BsnI	GGCC	2 cut(s)	121, 218
Bso31I	GGTCTC	1 cut(s)	912
Bsp1286I	GDGCHC	5 cut(s)	157, 285, 329, 449, 718
Bsp13I	TCCGGA	1 cut(s)	547
Bsp143I	GATC	5 cut(s)	244, 250, 296, 331, 896
BspACI	CCGC	7 cut(s)	134, 590, 722, 782, 788, 833, 845
BspANI	GGCC	2 cut(s)	121, 218
BspCNI	CTCAG	1 cut(s)	40
BspEI	TCCGGA	1 cut(s)	547
BspFNI	CGCG	1 cut(s)	724
BspLI	GGNNCC	2 cut(s)	298, 546
BspMI	ACCTGC	1 cut(s)	817
BspPI	GGATC	4 cut(s)	239, 291, 304, 326
BspTNI	GGTCTC	1 cut(s)	912
BsrBI	CCGCTC	1 cut(s)	845
BsrDI	GCAATG	1 cut(s)	902
BsrFI	RCCGGY	1 cut(s)	239
BsrI	ACTGG	1 cut(s)	888
BssAI	RCCGGY	1 cut(s)	239
BssECI	CCNNGG	4 cut(s)	128, 378, 532, 669
BssMI	GATC	5 cut(s)	244, 250, 296, 331, 896
BssSI	CACGAG	1 cut(s)	150
BssT1I	CCWWGG	2 cut(s)	128, 378
Bst2BI	CACGAG	1 cut(s)	150
Bst2UI	CCWGG	1 cut(s)	689
Bst4CI	ACNGT	2 cut(s)	324, 347
BstAPI	GCANNNNNTGC	1 cut(s)	324
BstBAI	YACGTR	1 cut(s)	359
BstC8I	GCNNGC	1 cut(s)	241
BstDEI	CTNAG	1 cut(s)	48
BstDSI	CCRYGG	1 cut(s)	532
BstEII	GGTNACC	1 cut(s)	706
BstF5I	GGATG	2 cut(s)	556, 853
BstFNI	CGCG	1 cut(s)	724
BstHHI	GCGC	1 cut(s)	700
BstKTI	GATC	5 cut(s)	247, 253, 299, 334, 899
BstMAI	GTCTC	1 cut(s)	912
BstMBI	GATC	5 cut(s)	244, 250, 296, 331, 896
BstMWI	GCNNNNNNNGC	3 cut(s)	324, 823, 851
BstNI	CCWGG	1 cut(s)	689
BstNSI	RCATGY	1 cut(s)	821
BstPI	GGTNACC	1 cut(s)	706
BstSCI	CCNGG	1 cut(s)	687
BstSLI	GKGCMC	2 cut(s)	157, 449
BstUI	CGCG	1 cut(s)	724
BstV1I	GCAGC	1 cut(s)	603
BstX2I	RGATCY	2 cut(s)	296, 896
BstYI	RGATCY	2 cut(s)	296, 896
BsuI	GTATCC	1 cut(s)	36
BsuRI	GGCC	2 cut(s)	121, 218
BtgI	CCRYGG	1 cut(s)	532
BtsCI	GGATG	2 cut(s)	556, 853
BtsI	GCAGTG	1 cut(s)	234
BtsIMutI	CAGTG	1 cut(s)	234
BveI	ACCTGC	1 cut(s)	817
Cac8I	GCNNGC	1 cut(s)	241
CfoI	GCGC	1 cut(s)	700
Cfr10I	RCCGGY	1 cut(s)	239
Cfr13I	GGNCC	1 cut(s)	347
CpoI	CGGWCCG	1 cut(s)	347
CseI	GACGC	1 cut(s)	357
CsiI	ACCWGGT	1 cut(s)	687
Csp6I	GTAC	1 cut(s)	881
CspI	CGGWCCG	1 cut(s)	347
CviAII	CATG	2 cut(s)	505, 818
CviQI	GTAC	1 cut(s)	881
DdeI	CTNAG	1 cut(s)	48
DpnI	GATC	5 cut(s)	246, 252, 298, 333, 898
DpnII	GATC	5 cut(s)	244, 250, 296, 331, 896
DrdI	GACNNNNNNGTC	1 cut(s)	350
DseDI	GACNNNNNNGTC	1 cut(s)	350
EaeI	YGGCCR	1 cut(s)	119
EciI	GGCGGA	1 cut(s)	848
Ecl136II	GAGCTC	1 cut(s)	716
Eco130I	CCWWGG	2 cut(s)	128, 378
Eco24I	GRGCYC	1 cut(s)	718
Eco31I	GGTCTC	1 cut(s)	912
Eco47I	GGWCC	1 cut(s)	347
Eco53kI	GAGCTC	1 cut(s)	716
Eco57I	CTGAAG	3 cut(s)	11, 296, 348
Eco91I	GGTNACC	1 cut(s)	706
EcoICRI	GAGCTC	1 cut(s)	716
EcoO65I	GGTNACC	1 cut(s)	706
EcoRII	CCWGG	1 cut(s)	687
EcoT14I	CCWWGG	2 cut(s)	128, 378
EcoT38I	GRGCYC	1 cut(s)	718
ErhI	CCWWGG	2 cut(s)	128, 378
FaeI	CATG	2 cut(s)	508, 821
FaiI	YATR	6 cut(s)	170, 320, 356, 489, 506, 819
FaqI	GGGAC	1 cut(s)	403
FatI	CATG	2 cut(s)	504, 817
FblI	GTMKAC	1 cut(s)	509
Fnu4HI	GCNGC	3 cut(s)	135, 591, 617
FokI	GGATG	2 cut(s)	563, 860
FriOI	GRGCYC	1 cut(s)	718
Fsp4HI	GCNGC	3 cut(s)	135, 591, 617
FspBI	CTAG	6 cut(s)	39, 113, 159, 314, 341, 894
GlaI	GCGC	1 cut(s)	699
GluI	GCNGC	3 cut(s)	135, 591, 617
GsaI	CCCAGC	2 cut(s)	439, 704
HaeIII	GGCC	2 cut(s)	121, 218
HapII	CCGG	5 cut(s)	240, 350, 548, 711, 812
HgaI	GACGC	1 cut(s)	357
HhaI	GCGC	1 cut(s)	700
Hin1II	CATG	2 cut(s)	508, 821
Hin6I	GCGC	1 cut(s)	698
HinP1I	GCGC	1 cut(s)	698
HinfI	GANTC	4 cut(s)	287, 528, 745, 926
HpaII	CCGG	5 cut(s)	240, 350, 548, 711, 812
HphI	GGTGA	4 cut(s)	133, 700, 703, 781
Hpy166II	GTNNAC	3 cut(s)	510, 685, 730
Hpy188I	TCNGA	9 cut(s)	19, 30, 301, 331, 485, 497, 665, 673, 744
Hpy188III	TCNNGA	5 cut(s)	106, 248, 256, 548, 751
Hpy8I	GTNNAC	3 cut(s)	510, 685, 730
HpyAV	CCTTC	5 cut(s)	35, 229, 271, 668, 871
HpyCH4III	ACNGT	2 cut(s)	324, 347
HpyCH4IV	ACGT	3 cut(s)	358, 373, 603
HpyCH4V	TGCA	2 cut(s)	77, 907
HpyF10VI	GCNNNNNNNGC	3 cut(s)	324, 823, 851
HpyF3I	CTNAG	1 cut(s)	48
HpySE526I	ACGT	3 cut(s)	358, 373, 603
Hsp92II	CATG	2 cut(s)	508, 821
HspAI	GCGC	1 cut(s)	698
Kpn2I	TCCGGA	1 cut(s)	547
KroI	GCCGGC	1 cut(s)	239
KroNI	GCCGGC	1 cut(s)	241
Kzo9I	GATC	5 cut(s)	244, 250, 296, 331, 896
LmnI	GCTCC	4 cut(s)	332, 713, 814, 842
Lsp1109I	GCAGC	1 cut(s)	603
LweI	GCATC	2 cut(s)	86, 501
MabI	ACCWGGT	1 cut(s)	687
MaeI	CTAG	6 cut(s)	39, 113, 159, 314, 341, 894
MaeII	ACGT	3 cut(s)	358, 373, 603
MaeIII	GTNAC	3 cut(s)	369, 706, 769
MalI	GATC	5 cut(s)	246, 252, 298, 333, 898
MbiI	CCGCTC	1 cut(s)	845
MboI	GATC	5 cut(s)	244, 250, 296, 331, 896
MboII	GAAGA	3 cut(s)	59, 434, 607
MflI	RGATCY	2 cut(s)	296, 896
MhlI	GDGCHC	5 cut(s)	157, 285, 329, 449, 718
MlsI	TGGCCA	1 cut(s)	121
MluCI	AATT	1 cut(s)	194
MluNI	TGGCCA	1 cut(s)	121
MlyI	GAGTC	1 cut(s)	537
MnlI	CCTC	6 cut(s)	155, 346, 380, 679, 747, 760
Mox20I	TGGCCA	1 cut(s)	121
MroI	TCCGGA	1 cut(s)	547
MroNI	GCCGGC	1 cut(s)	239
MscI	TGGCCA	1 cut(s)	121
MseI	TTAA	2 cut(s)	92, 762
MslI	CAYNNNNRTG	1 cut(s)	413
Msp20I	TGGCCA	1 cut(s)	121
MspA1I	CMGCKG	1 cut(s)	439
MspI	CCGG	5 cut(s)	240, 350, 548, 711, 812
MspR9I	CCNGG	1 cut(s)	689
MvaI	CCWGG	1 cut(s)	689
MvnI	CGCG	1 cut(s)	724
MwoI	GCNNNNNNNGC	3 cut(s)	324, 823, 851
NaeI	GCCGGC	1 cut(s)	241
NdeII	GATC	5 cut(s)	244, 250, 296, 331, 896
NgoMIV	GCCGGC	1 cut(s)	239
NlaIII	CATG	2 cut(s)	508, 821
NlaIV	GGNNCC	2 cut(s)	298, 546
NmuCI	GTSAC	3 cut(s)	369, 706, 769
NspI	RCATGY	1 cut(s)	821
PaqCI	CACCTGC	1 cut(s)	817
PdiI	GCCGGC	1 cut(s)	241
PfeI	GAWTC	3 cut(s)	287, 745, 926
PkrI	GCNGC	3 cut(s)	136, 592, 618
PleI	GAGTC	1 cut(s)	536
PpsI	GAGTC	1 cut(s)	536
Ppu21I	YACGTR	1 cut(s)	359
Psp124BI	GAGCTC	1 cut(s)	718
Psp1406I	AACGTT	1 cut(s)	603
Psp6I	CCWGG	1 cut(s)	687
PspEI	GGTNACC	1 cut(s)	706
PspFI	CCCAGC	2 cut(s)	435, 700
PspGI	CCWGG	1 cut(s)	687
PspN4I	GGNNCC	2 cut(s)	298, 546
PspPI	GGNCC	1 cut(s)	347
PsuI	RGATCY	2 cut(s)	296, 896
PvuII	CAGCTG	1 cut(s)	439
RsaI	GTAC	1 cut(s)	882
RsaNI	GTAC	1 cut(s)	881
RseI	CAYNNNNRTG	1 cut(s)	413
Rsr2I	CGGWCCG	1 cut(s)	347
RsrII	CGGWCCG	1 cut(s)	347
SacI	GAGCTC	1 cut(s)	718
SaqAI	TTAA	2 cut(s)	92, 762
SatI	GCNGC	3 cut(s)	135, 591, 617
Sau3AI	GATC	5 cut(s)	244, 250, 296, 331, 896
Sau96I	GGNCC	1 cut(s)	347
SchI	GAGTC	1 cut(s)	537
ScrFI	CCNGG	1 cut(s)	689
SduI	GDGCHC	5 cut(s)	157, 285, 329, 449, 718
SexAI	ACCWGGT	1 cut(s)	687
SfaNI	GCATC	2 cut(s)	86, 501
SinI	GGWCC	1 cut(s)	347
SmiMI	CAYNNNNRTG	1 cut(s)	413
SmlI	CTYRAG	1 cut(s)	751
SmoI	CTYRAG	1 cut(s)	751
Sse9I	AATT	1 cut(s)	194
SsiI	CCGC	7 cut(s)	134, 590, 722, 782, 788, 833, 845
SspMI	CTAG	6 cut(s)	39, 113, 159, 314, 341, 894
SstI	GAGCTC	1 cut(s)	718
StyD4I	CCNGG	1 cut(s)	687
StyI	CCWWGG	2 cut(s)	128, 378
TaaI	ACNGT	2 cut(s)	324, 347
TaiI	ACGT	3 cut(s)	361, 376, 606
TaqI	TCGA	3 cut(s)	285, 309, 806
TasI	AATT	1 cut(s)	194
TauI	GCSGC	2 cut(s)	137, 593
TfiI	GAWTC	3 cut(s)	287, 745, 926
Tru1I	TTAA	2 cut(s)	92, 762
Tru9I	TTAA	2 cut(s)	92, 762
TscAI	CASTG	1 cut(s)	241
TseFI	GTSAC	3 cut(s)	369, 706, 769
TseI	GCWGC	1 cut(s)	616
Tsp45I	GTSAC	3 cut(s)	369, 706, 769
TspDTI	ATGAA	6 cut(s)	47, 50, 211, 493, 600, 665
TspGWI	ACGGA	1 cut(s)	521
TspRI	CASTG	1 cut(s)	241
VpaK11BI	GGWCC	1 cut(s)	347
XceI	RCATGY	1 cut(s)	821
XmiI	GTMKAC	1 cut(s)	509
XspI	CTAG	6 cut(s)	39, 113, 159, 314, 341, 894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.