FvH4_6g14860

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002212
Physical Location & Seq
Reverse (-)
9214201 .. 9217159
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g14860.t2

Sequence Viewer

Length: 1500 bp
ATGATTTCCTTTGACTCCGAGATTGAAGAACAGGTGAAACTGGAATCTATGGTAGAGAAAAGTAGTTCCCTGGAAAAAGCAATCATGTCCGCGGAGCAATTGAAGCATGAGGAGAGTTCAATGGAAGCATCTTTGTCCCAAATGGCTACTGAATTGAAATATCATCAGAGAGATATAGTTGCTGCACATATTTTGGCCCAGATAGCCAATGAAGATTCTGGTTTGCTTTGTCACAGGTCAAGTTCCCATGGAGTGAAGTACAGCATGAAAGGGAAGCCGAGAATGAGGTGGGAAACGAAAGGTGATAGAATAGGAGGGGGTGATAGTCATTCTGAAGTGGCTAGGAAAAAGGCCAAGTTTGTTGCAAAGCGCCAGCAAGAGGCGGTTGGAGGTCAGATGAATGCTGCAGATAGTGTAGTCTTAGCTTTATCAGCAATGCCATGTTCTGGAAAGAGAAAACATAAAAAGATGAATGATTTATATGAAGTGGGTGGTTTTTTGGATGGTCATGAGCTGACTAATAGATGGGGAAACAAATCAAACAATCATCAGGAGTCATTTGAAGCTTGCATTGCGGATCATCATGGTAGATTAGGCGTGTTAAGAGAAGAAAATGTGCATGTTGCAGGCCCAGTAAACATACCAAGACATATGGAGAATGATGCCAGAGAGGAGAATGGTGACGATTTGCATGAGATGTGGCAGGTTTCGGATAGTCATGAGATCGCTGACAGATGGGGAAACAATGATCAGAAAGCAGTTGGAGCTGGTCTTGAGGATCCTCATGATAGATTAGGCATGTTAAGTATTAGGAAGCGGAAAAGTGTCGCAGGATCCTACGTCAAAAGAGATGCCAAGGATGTTGCAGTACCAGTGAAAATGCCCTTCAAATTGGCAGTTCCTACTGTTGAGAGACTTCATCCAGTTGGAAGCCCTCCTGCACCACCTCTAGTTTTGATGACACCAACATTATACAAAAGCAAAAGGTTTATAGGTGTGGGTTGTAGGAGGAGCATCGCAACTATCAACCCAAGTTCTGAAGAAGTAAGAGCTCACTTTCATAAGGAGGAAGCTTTGAGGTACTTATCCCCGGATAGGGGATTTTGTTACACAGCACTGGATGGTAGAAAGTCTGTTGTTGCTCCTATAAAAATAAGGAGCAAGGTATGCCTAGACCATCTTAAGCTTAAAGCTGATAGGCCGTGGGTTGTCTCTATCAACTGTCTTGTGCGAGATGCAGCTGCCAGATTGCCTGGAGGTATGGGTACCAGAGCAGATATATGTACTCTCATGCGGGACTCGCAGTATCTACTGGAAGATGTCACTGATGAACAACTTAACCATTATGCTAGCAAAGGACTGAACCGGCTCCACTGTGAAGTTGACCCATGCGTTAAGTATGATGGGAAGGTGTGGGTTTATTTACATCGAAATAGAGGGGTGGAAGACTTTGAGGAAGATGCTACCTTTCATACCGACTACTTAACGAGAAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

55.97

Weight (kDa)

7.98

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 334 - 464 3.8e-48 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 694
Acc65I GGTACC 1 cut(s) 1265
AccB1I GGYRCC 1 cut(s) 1265
AccB7I CCANNNNNTGG 1 cut(s) 446
AccII CGCG 1 cut(s) 92
AciI CCGC 6 cut(s) 90, 92, 383, 575, 817, 1294
AclWI GGATC 5 cut(s) 585, 773, 786, 828, 841
AcuI CTGAAG 2 cut(s) 354, 1059
AfaI GTAC 5 cut(s) 260, 870, 1082, 1267, 1285
AfiI CCNNNNNNNGG 5 cut(s) 379, 446, 1095, 1096, 1097
AflII CTTAAG 1 cut(s) 1181
AgsI TTSAA 6 cut(s) 26, 103, 120, 157, 563, 889
AjnI CCWGG 2 cut(s) 69, 1252
AluBI AGCT 9 cut(s) 425, 514, 566, 767, 1052, 1073, 1186, 1193, 1241
AluI AGCT 9 cut(s) 425, 514, 566, 767, 1052, 1073, 1186, 1193, 1241
Alw21I GWGCWC 1 cut(s) 1054
Alw26I GTCTC 2 cut(s) 907, 1216
AlwI GGATC 5 cut(s) 585, 773, 786, 828, 841
AoxI GGCC 4 cut(s) 195, 351, 628, 1199
ApeKI GCWGC 4 cut(s) 182, 404, 1238, 1241
Asp718I GGTACC 1 cut(s) 1265
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 196, 629
AsuC2I CCSGG 1 cut(s) 1091
AsuHPI GGTGA 4 cut(s) 46, 314, 332, 692
AsuNHI GCTAGC 1 cut(s) 1349
BamHI GGATCC 2 cut(s) 778, 833
BanI GGYRCC 1 cut(s) 1265
BanII GRGCYC 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 1452
Bbv12I GWGCWC 1 cut(s) 1054
BbvI GCAGC 4 cut(s) 169, 391, 1228, 1250
BccI CCATC 6 cut(s) 497, 519, 729, 1115, 1185, 1397
BceAI ACGGC 1 cut(s) 1186
BciT130I CCWGG 2 cut(s) 71, 1254
BclI TGATCA 1 cut(s) 748
BcnI CCSGG 1 cut(s) 1091
BcoDI GTCTC 2 cut(s) 907, 1216
BfaI CTAG 4 cut(s) 342, 950, 1172, 1350
BfmI CTRYAG 1 cut(s) 405
BfoI RGCGCY 1 cut(s) 373
BfrI CTTAAG 1 cut(s) 1181
BfuAI ACCTGC 1 cut(s) 694
BisI GCNGC 4 cut(s) 183, 405, 1239, 1242
BlsI GCNGC 4 cut(s) 184, 406, 1240, 1243
Bme1390I CCNGG 3 cut(s) 71, 1091, 1254
BmgT120I GGNCC 2 cut(s) 196, 629
BmiI GGNNCC 4 cut(s) 780, 835, 1267, 1370
BmrFI CCNGG 3 cut(s) 71, 1091, 1254
BmrI ACTGGG 1 cut(s) 626
BmsI GCATC 6 cut(s) 137, 652, 841, 1023, 1225, 1450
BmtI GCTAGC 1 cut(s) 1353
BmuI ACTGGG 1 cut(s) 626
BpiI GAAGAC 1 cut(s) 1452
BpmI CTGGAG 1 cut(s) 1275
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 1091
BsaJI CCNNGG 6 cut(s) 69, 90, 247, 855, 1089, 1202
Bsc4I CCNNNNNNNGG 5 cut(s) 379, 446, 1095, 1096, 1097
Bse118I RCCGGY 1 cut(s) 1365
Bse1I ACTGG 6 cut(s) 45, 632, 872, 923, 1122, 1317
Bse3DI GCAATG 2 cut(s) 441, 570
BseBI CCWGG 2 cut(s) 71, 1254
BseDI CCNNGG 6 cut(s) 69, 90, 247, 855, 1089, 1202
BseGI GGATG 4 cut(s) 508, 865, 919, 1126
BseLI CCNNNNNNNGG 5 cut(s) 379, 446, 1095, 1096, 1097
BseMI GCAATG 2 cut(s) 441, 570
BseNI ACTGG 6 cut(s) 45, 632, 872, 923, 1122, 1317
BseRI GAGGAG 3 cut(s) 125, 686, 1024
BseXI GCAGC 4 cut(s) 169, 391, 1228, 1250
BsgI GTGCAG 2 cut(s) 168, 924
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 4 cut(s) 197, 353, 630, 1201
BshNI GGYRCC 1 cut(s) 1265
BsiHKAI GWGCWC 1 cut(s) 1054
BsiSI CCGG 2 cut(s) 1091, 1366
BslFI GGGAC 2 cut(s) 121, 1310
BslI CCNNNNNNNGG 5 cut(s) 379, 446, 1095, 1096, 1097
BsmAI GTCTC 2 cut(s) 907, 1216
BsmFI GGGAC 2 cut(s) 121, 1310
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 4 cut(s) 197, 353, 630, 1201
Bsp1286I GDGCHC 1 cut(s) 1054
Bsp143I GATC 5 cut(s) 577, 723, 748, 778, 833
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 6 cut(s) 90, 92, 383, 575, 817, 1294
BspANI GGCC 4 cut(s) 197, 353, 630, 1201
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 3 cut(s) 508, 718, 784
BspLI GGNNCC 4 cut(s) 780, 835, 1267, 1370
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 694
BspOI GCTAGC 1 cut(s) 1353
BspPI GGATC 5 cut(s) 585, 773, 786, 828, 841
BspT107I GGYRCC 1 cut(s) 1265
BspTI CTTAAG 1 cut(s) 1181
BsrDI GCAATG 2 cut(s) 441, 570
BsrFI RCCGGY 1 cut(s) 1365
BsrI ACTGG 6 cut(s) 45, 632, 872, 923, 1122, 1317
BssAI RCCGGY 1 cut(s) 1365
BssECI CCNNGG 6 cut(s) 69, 90, 247, 855, 1089, 1202
BssMI GATC 5 cut(s) 577, 723, 748, 778, 833
BssT1I CCWWGG 2 cut(s) 247, 855
Bst2UI CCWGG 2 cut(s) 71, 1254
Bst4CI ACNGT 3 cut(s) 907, 1223, 1376
BstAFI CTTAAG 1 cut(s) 1181
BstAPI GCANNNNNTGC 1 cut(s) 1167
BstC8I GCNNGC 4 cut(s) 374, 568, 628, 1351
BstDEI CTNAG 1 cut(s) 421
BstDSI CCRYGG 3 cut(s) 90, 247, 1202
BstF5I GGATG 4 cut(s) 508, 865, 919, 1126
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 373
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 5 cut(s) 580, 726, 751, 781, 836
BstMAI GTCTC 2 cut(s) 907, 1216
BstMBI GATC 5 cut(s) 577, 723, 748, 778, 833
BstMWI GCNNNNNNNGC 7 cut(s) 103, 203, 431, 572, 764, 1167, 1300
BstNI CCWGG 2 cut(s) 71, 1254
BstNSI RCATGY 2 cut(s) 623, 802
BstSCI CCNGG 3 cut(s) 69, 1089, 1252
BstSFI CTRYAG 1 cut(s) 405
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 4 cut(s) 169, 391, 1228, 1250
BstV2I GAAGAC 1 cut(s) 1452
BstX2I RGATCY 2 cut(s) 778, 833
BstYI RGATCY 2 cut(s) 778, 833
BsuRI GGCC 4 cut(s) 197, 353, 630, 1201
BtgI CCRYGG 3 cut(s) 90, 247, 1202
BtgZI GCGATG 1 cut(s) 1000
BtsCI GGATG 4 cut(s) 508, 865, 919, 1126
BtsIMutI CAGTG 4 cut(s) 879, 1115, 1323, 1372
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 4 cut(s) 374, 568, 628, 1351
CciI TCATGA 3 cut(s) 508, 718, 784
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 1 cut(s) 1365
Cfr13I GGNCC 2 cut(s) 196, 629
Cfr42I CCGCGG 1 cut(s) 93
Csp6I GTAC 5 cut(s) 259, 869, 1081, 1266, 1284
CviQI GTAC 5 cut(s) 259, 869, 1081, 1266, 1284
DdeI CTNAG 1 cut(s) 421
DpnI GATC 5 cut(s) 579, 725, 750, 780, 835
DpnII GATC 5 cut(s) 577, 723, 748, 778, 833
Ecl136II GAGCTC 1 cut(s) 1052
Eco130I CCWWGG 2 cut(s) 247, 855
Eco24I GRGCYC 1 cut(s) 1054
Eco53kI GAGCTC 1 cut(s) 1052
Eco57I CTGAAG 2 cut(s) 354, 1059
EcoICRI GAGCTC 1 cut(s) 1052
EcoRII CCWGG 2 cut(s) 69, 1252
EcoT14I CCWWGG 2 cut(s) 247, 855
EcoT38I GRGCYC 1 cut(s) 1054
ErhI CCWWGG 2 cut(s) 247, 855
FaqI GGGAC 2 cut(s) 121, 1310
FauI CCCGC 1 cut(s) 1287
FauNDI CATATG 1 cut(s) 651
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 4 cut(s) 183, 405, 1239, 1242
FokI GGATG 4 cut(s) 515, 872, 906, 1133
FriOI GRGCYC 1 cut(s) 1054
Fsp4HI GCNGC 4 cut(s) 183, 405, 1239, 1242
FspBI CTAG 4 cut(s) 342, 950, 1172, 1350
GlaI GCGC 1 cut(s) 371
GluI GCNGC 4 cut(s) 183, 405, 1239, 1242
GsuI CTGGAG 1 cut(s) 1275
HaeII RGCGCY 1 cut(s) 373
HaeIII GGCC 4 cut(s) 197, 353, 630, 1201
HapII CCGG 2 cut(s) 1091, 1366
HhaI GCGC 1 cut(s) 372
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 1 cut(s) 1384
HindII GTYRAC 1 cut(s) 1384
HindIII AAGCTT 3 cut(s) 564, 1071, 1184
HinfI GANTC 5 cut(s) 14, 44, 215, 554, 1298
HpaII CCGG 2 cut(s) 1091, 1366
HphI GGTGA 4 cut(s) 46, 314, 332, 692
Hpy166II GTNNAC 2 cut(s) 637, 1384
Hpy188I TCNGA 7 cut(s) 19, 168, 334, 396, 712, 753, 1039
Hpy188III TCNNGA 6 cut(s) 447, 509, 551, 719, 773, 785
Hpy8I GTNNAC 2 cut(s) 637, 1384
HpyAV CCTTC 2 cut(s) 895, 1402
HpyCH4III ACNGT 3 cut(s) 907, 1223, 1376
HpyCH4IV ACGT 1 cut(s) 840
HpyF10VI GCNNNNNNNGC 7 cut(s) 103, 203, 431, 572, 764, 1167, 1300
HpyF3I CTNAG 1 cut(s) 421
HpySE526I ACGT 1 cut(s) 840
HspAI GCGC 1 cut(s) 370
KpnI GGTACC 1 cut(s) 1269
Ksp22I TGATCA 1 cut(s) 748
KspI CCGCGG 1 cut(s) 93
Kzo9I GATC 5 cut(s) 577, 723, 748, 778, 833
LmnI GCTCC 6 cut(s) 94, 764, 1011, 1147, 1158, 1374
Lsp1109I GCAGC 4 cut(s) 169, 391, 1228, 1250
LweI GCATC 6 cut(s) 137, 652, 841, 1023, 1225, 1450
MaeI CTAG 4 cut(s) 342, 950, 1172, 1350
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 4 cut(s) 230, 680, 1106, 1321
MalI GATC 5 cut(s) 579, 725, 750, 780, 835
MboI GATC 5 cut(s) 577, 723, 748, 778, 833
MboII GAAGA 7 cut(s) 38, 224, 620, 1052, 1328, 1457, 1469
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 2 cut(s) 778, 833
MhlI GDGCHC 1 cut(s) 1054
MluCI AATT 3 cut(s) 98, 152, 890
MlyI GAGTC 3 cut(s) 8, 563, 1292
MmeI TCCRAC 3 cut(s) 367, 742, 907
MseI TTAA 7 cut(s) 602, 803, 1182, 1188, 1338, 1395, 1484
MspA1I CMGCKG 2 cut(s) 92, 1241
MspCI CTTAAG 1 cut(s) 1181
MspI CCGG 2 cut(s) 1091, 1366
MspR9I CCNGG 3 cut(s) 71, 1091, 1254
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 406
MvaI CCWGG 2 cut(s) 71, 1254
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 103, 203, 431, 572, 764, 1167, 1300
NciI CCSGG 1 cut(s) 1091
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 1 cut(s) 651
NdeII GATC 5 cut(s) 577, 723, 748, 778, 833
NheI GCTAGC 1 cut(s) 1349
NlaIV GGNNCC 4 cut(s) 780, 835, 1267, 1370
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 3 cut(s) 230, 680, 1321
NspI RCATGY 2 cut(s) 623, 802
PagI TCATGA 3 cut(s) 508, 718, 784
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 2 cut(s) 44, 215
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 4 cut(s) 184, 406, 1240, 1243
PleI GAGTC 3 cut(s) 8, 562, 1292
PpsI GAGTC 3 cut(s) 8, 562, 1292
Psp124BI GAGCTC 1 cut(s) 1054
Psp6I CCWGG 2 cut(s) 69, 1252
PspGI CCWGG 2 cut(s) 69, 1252
PspN4I GGNNCC 4 cut(s) 780, 835, 1267, 1370
PspPI GGNCC 2 cut(s) 196, 629
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 2 cut(s) 778, 833
PvuII CAGCTG 1 cut(s) 1241
RsaI GTAC 5 cut(s) 260, 870, 1082, 1267, 1285
RsaNI GTAC 5 cut(s) 259, 869, 1081, 1266, 1284
SacI GAGCTC 1 cut(s) 1054
SacII CCGCGG 1 cut(s) 93
SaqAI TTAA 7 cut(s) 602, 803, 1182, 1188, 1338, 1395, 1484
SatI GCNGC 4 cut(s) 183, 405, 1239, 1242
Sau3AI GATC 5 cut(s) 577, 723, 748, 778, 833
Sau96I GGNCC 2 cut(s) 196, 629
SchI GAGTC 3 cut(s) 8, 563, 1292
ScrFI CCNGG 3 cut(s) 71, 1091, 1254
SduI GDGCHC 1 cut(s) 1054
SfaNI GCATC 6 cut(s) 137, 652, 841, 1023, 1225, 1450
SfcI CTRYAG 1 cut(s) 405
Sfr303I CCGCGG 1 cut(s) 93
SgrBI CCGCGG 1 cut(s) 93
SmlI CTYRAG 2 cut(s) 773, 1181
SmoI CTYRAG 2 cut(s) 773, 1181
Sse9I AATT 3 cut(s) 98, 152, 890
SsiI CCGC 6 cut(s) 90, 92, 383, 575, 817, 1294
SspMI CTAG 4 cut(s) 342, 950, 1172, 1350
SstI GAGCTC 1 cut(s) 1054
StyD4I CCNGG 3 cut(s) 69, 1089, 1252
StyI CCWWGG 2 cut(s) 247, 855
TaaI ACNGT 3 cut(s) 907, 1223, 1376
TaiI ACGT 1 cut(s) 843
TaqI TCGA 1 cut(s) 1429
TasI AATT 3 cut(s) 98, 152, 890
TatI WGTACW 2 cut(s) 258, 1283
TfiI GAWTC 2 cut(s) 44, 215
Tru1I TTAA 7 cut(s) 602, 803, 1182, 1188, 1338, 1395, 1484
Tru9I TTAA 7 cut(s) 602, 803, 1182, 1188, 1338, 1395, 1484
TscAI CASTG 4 cut(s) 879, 1122, 1330, 1379
TseFI GTSAC 3 cut(s) 230, 680, 1321
TseI GCWGC 4 cut(s) 182, 404, 1238, 1241
Tsp45I GTSAC 3 cut(s) 230, 680, 1321
TspDTI ATGAA 9 cut(s) 225, 281, 413, 485, 498, 908, 1049, 1344, 1460
TspRI CASTG 4 cut(s) 879, 1122, 1330, 1379
Van91I CCANNNNNTGG 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 1181
XceI RCATGY 2 cut(s) 623, 802
XspI CTAG 4 cut(s) 342, 950, 1172, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.