Rroxscaffold_6G00413080

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002212
Physical Location & Seq
Forward (+)
35499255 .. 35502371
3117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00413080.1

Sequence Viewer

Length: 1569 bp
ATGATTGCCTTTGACTCCGAGATTGAAGAACCAATGAAACTGGAGAGTAAGGTAGAGAAAAGTAATTGCCTGGAAGAAGAAATCATGTCAGCAGAGCAATTGAAGCATGAGGAGAGATCAATGGAAGCATCATCATGTCAAATGGCTACTGAATTGAAATGTCAGCGGAGAGATATAGTTGCTGCAAATATTTTGGTCCAGATGGCCAATGAAGATTCTGCTTTGCTTCATCACAGATTAAGTTTGCATGGAGTGAAGCATAGCATGAAAGGGAAGCCGAGAATGAGGTGGGAAAAGAAAGGTGATAGAATAGGAGTGGGTGATAATGATTCCAAAGTGGCTAGGAGAGGGGCCAAGTTTATTGCAAAGCGTCAGCAAGCTGCGTTTGGAGGTCAGACGAATGATGCAGATAGTGTAGTCTTAGCTTTATCAGCAGTGCCACGTTCTGGAAGGAGAAAGAATAGAAAGATGAACATCGGTATGGAACTTTCTTCAGTCCAACCAAATTATATAAATACTAATGTCAGTGATAACAAGGATAAAACTTTATGTGAAGCGTGTGGAGTTTTGGATGGTGATGAGCTGACTAGTAGATGGGGAAACGATCATCGCAAGTCATTTAAAGCTTGTGTTGAGGATTGTTATGATAGATTAGGTGTGTTGAGAGAAAAAAATGTGGATGTTGCAGGCTCAGTAAACATACCAAGACATGTGAAATATACTGTCAGTGAGGAGAAGGACGACAATTTGTACGAGGCACGGCAGTTTTGGGATGGTCATGAGCTGACTGAGAAGTGGGGAAACAATGATCAGAAATCAGTTGAAGCTGGTCTTGAGGATCGTCATGATAGATTAGGCATGTTAAGTATAAAGAAGAGGAAAAGAGTGGCAGGATCCCACTTTAAAAGAGATGCCGAGCATGTTGCAGTACCGGGGAAAATACCATTGAAAATGCCCTTGAAATTGGCAGTTCCTATTGTTGAGAGACTTCATCCAGTTGGAAGCCCTTCTGCACCACCTCTGGTTTTGATGAGACCAACATTAAACAAAAGGAAAAGGTTTAGGGGTGTGGGCCGTAAGAGGAGCATCGCAACTATCAGCCCAAGCTCTGAAGAATTAAGAGCTTACTTTCATAATGAGGAGGCTTTGAGGTACTTAGTCCCCGATAGGGGATTTTGTTACACAGCACTGGATGGTAGAAAGTCTGCAGTCGCTCCTCTACGGAAGGTGAGCAACATACACCGAGACCATGTTATGCTTAAAGCTGATAGGCCGCATGTTGTTTCCGTCTGCTGTCTTGTGCGGGATGCAGCTGCTAGATTGCCTGGAGGTATGGGTACCAGAACAGATATATGTACTCTCATGCGGGACTCACAGTATCTAGTCGAAGATGTCACTGATCAACAACTTCATCATTTTGCAAGCAAAGGACTAGACAGGCTGCACTATGAAGTTGATCCCTGCGTCAAGTATGATGGGAAGTTGTGGGTTTATTTACATCGACATAGAGGGGAGGAGGACTTTGAGGATGATGCTACGTTATGTTCTGTCTACTTAAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

522

Amino Acids

59.24

Weight (kDa)

9.04

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 374 - 506 6.1e-53 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1337
AccB1I GGYRCC 1 cut(s) 1337
AccB7I CCANNNNNTGG 1 cut(s) 446
AccI GTMKAC 1 cut(s) 1551
AciI CCGC 4 cut(s) 166, 1274, 1303, 1366
AclWI GGATC 4 cut(s) 846, 888, 901, 1451
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 2 cut(s) 477, 1131
AfaI GTAC 5 cut(s) 752, 930, 1154, 1339, 1357
AfiI CCNNNNNNNGG 3 cut(s) 446, 1168, 1169
AflII CTTAAG 1 cut(s) 1555
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 6 cut(s) 26, 103, 157, 824, 949, 961
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 2 cut(s) 69, 1324
Alw26I GTCTC 3 cut(s) 979, 1027, 1239
AlwI GGATC 4 cut(s) 846, 888, 901, 1451
AoxI GGCC 4 cut(s) 204, 351, 1072, 1271
ApeKI GCWGC 5 cut(s) 182, 380, 1310, 1313, 1441
Asp700I GAANNNNTTC 1 cut(s) 1006
Asp718I GGTACC 1 cut(s) 1337
AspS9I GGNCC 3 cut(s) 196, 351, 1072
AsuC2I CCSGG 1 cut(s) 933
AsuHPI GGTGA 4 cut(s) 314, 332, 587, 1240
AvaII GGWCC 1 cut(s) 196
BalI TGGCCA 1 cut(s) 206
BamHI GGATCC 1 cut(s) 893
BanI GGYRCC 1 cut(s) 1337
BbvI GCAGC 5 cut(s) 169, 367, 1300, 1322, 1428
BccI CCATC 6 cut(s) 196, 566, 588, 767, 1187, 1469
BceAI ACGGC 2 cut(s) 776, 1059
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciT130I CCWGG 2 cut(s) 71, 1326
BclI TGATCA 2 cut(s) 808, 1399
BcnI CCSGG 1 cut(s) 933
BcoDI GTCTC 3 cut(s) 979, 1027, 1239
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 5 cut(s) 342, 588, 1317, 1382, 1433
BfmI CTRYAG 1 cut(s) 1206
BfrI CTTAAG 1 cut(s) 1555
BisI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
BlsI GCNGC 6 cut(s) 184, 382, 1275, 1312, 1315, 1443
Bme1390I CCNGG 3 cut(s) 71, 933, 1326
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 196, 351, 1072
BmiI GGNNCC 3 cut(s) 352, 895, 1339
BmrFI CCNGG 3 cut(s) 71, 933, 1326
BmsI GCATC 6 cut(s) 137, 394, 901, 1095, 1297, 1522
BpmI CTGGAG 2 cut(s) 62, 1347
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 1 cut(s) 933
BsaBI GATNNNNATC 1 cut(s) 473
BsaI GGTCTC 2 cut(s) 1027, 1239
BsaJI CCNNGG 1 cut(s) 932
Bsc4I CCNNNNNNNGG 3 cut(s) 446, 1168, 1169
Bse1I ACTGG 3 cut(s) 45, 995, 1194
Bse8I GATNNNNATC 1 cut(s) 473
BseBI CCWGG 2 cut(s) 71, 1326
BseDI CCNNGG 1 cut(s) 932
BseGI GGATG 7 cut(s) 577, 685, 778, 991, 1198, 1312, 1534
BseJI GATNNNNATC 1 cut(s) 473
BseLI CCNNNNNNNGG 3 cut(s) 446, 1168, 1169
BseMII CTCAG 2 cut(s) 705, 780
BseNI ACTGG 3 cut(s) 45, 995, 1194
BseRI GAGGAG 6 cut(s) 125, 746, 1096, 1154, 1206, 1529
BseXI GCAGC 5 cut(s) 169, 367, 1300, 1322, 1428
BsgI GTGCAG 2 cut(s) 996, 1427
BshFI GGCC 4 cut(s) 206, 353, 1074, 1273
BshNI GGYRCC 1 cut(s) 1337
BsiSI CCGG 1 cut(s) 932
BslFI GGGAC 2 cut(s) 1145, 1382
BslI CCNNNNNNNGG 3 cut(s) 446, 1168, 1169
BsmAI GTCTC 3 cut(s) 979, 1027, 1239
BsmFI GGGAC 2 cut(s) 1145, 1382
BsnI GGCC 4 cut(s) 206, 353, 1074, 1273
Bso31I GGTCTC 2 cut(s) 1027, 1239
Bsp143I GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
BspACI CCGC 4 cut(s) 166, 1274, 1303, 1366
BspANI GGCC 4 cut(s) 206, 353, 1074, 1273
BspCNI CTCAG 2 cut(s) 704, 781
BspHI TCATGA 2 cut(s) 778, 844
BspLI GGNNCC 3 cut(s) 352, 895, 1339
BspMAI CTGCAG 1 cut(s) 1210
BspPI GGATC 4 cut(s) 846, 888, 901, 1451
BspT107I GGYRCC 1 cut(s) 1337
BspTI CTTAAG 1 cut(s) 1555
BspTNI GGTCTC 2 cut(s) 1027, 1239
BsrI ACTGG 3 cut(s) 45, 995, 1194
BssECI CCNNGG 1 cut(s) 932
BssMI GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
Bst2UI CCWGG 2 cut(s) 71, 1326
Bst4CI ACNGT 2 cut(s) 724, 1377
Bst6I CTCTTC 1 cut(s) 869
BstAFI CTTAAG 1 cut(s) 1555
BstC8I GCNNGC 3 cut(s) 378, 688, 1423
BstDEI CTNAG 4 cut(s) 421, 691, 789, 1156
BstF5I GGATG 7 cut(s) 577, 685, 778, 991, 1198, 1312, 1534
BstKTI GATC 7 cut(s) 119, 607, 811, 841, 896, 1402, 1459
BstMAI GTCTC 3 cut(s) 979, 1027, 1239
BstMBI GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
BstMWI GCNNNNNNNGC 2 cut(s) 103, 431
BstNI CCWGG 2 cut(s) 71, 1326
BstNSI RCATGY 4 cut(s) 713, 862, 923, 1280
BstSCI CCNGG 3 cut(s) 69, 931, 1324
BstSFI CTRYAG 1 cut(s) 1206
BstV1I GCAGC 5 cut(s) 169, 367, 1300, 1322, 1428
BstX2I RGATCY 1 cut(s) 893
BstYI RGATCY 1 cut(s) 893
BsuRI GGCC 4 cut(s) 206, 353, 1074, 1273
BtgZI GCGATG 2 cut(s) 593, 1072
BtsCI GGATG 7 cut(s) 577, 685, 778, 991, 1198, 1312, 1534
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 5 cut(s) 441, 532, 733, 1187, 1395
Cac8I GCNNGC 3 cut(s) 378, 688, 1423
CciI TCATGA 2 cut(s) 778, 844
Cfr13I GGNCC 3 cut(s) 196, 351, 1072
CseI GACGC 2 cut(s) 359, 1453
Csp6I GTAC 5 cut(s) 751, 929, 1153, 1338, 1356
CviQI GTAC 5 cut(s) 751, 929, 1153, 1338, 1356
DdeI CTNAG 4 cut(s) 421, 691, 789, 1156
DpnI GATC 7 cut(s) 118, 606, 810, 840, 895, 1401, 1458
DpnII GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
DraI TTTAAA 2 cut(s) 622, 904
EaeI YGGCCR 1 cut(s) 204
Eam1104I CTCTTC 1 cut(s) 869
EarI CTCTTC 1 cut(s) 869
Eco31I GGTCTC 2 cut(s) 1027, 1239
Eco47I GGWCC 1 cut(s) 196
Eco57I CTGAAG 2 cut(s) 477, 1131
EcoRII CCWGG 2 cut(s) 69, 1324
FalI AAGNNNNNCTT 2 cut(s) 816, 848
FaqI GGGAC 2 cut(s) 1145, 1382
FauI CCCGC 2 cut(s) 1296, 1359
FbaI TGATCA 2 cut(s) 808, 1399
FblI GTMKAC 1 cut(s) 1551
Fnu4HI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
FokI GGATG 7 cut(s) 584, 692, 785, 978, 1205, 1319, 1541
Fsp4HI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
FspBI CTAG 5 cut(s) 342, 588, 1317, 1382, 1433
GluI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
GsuI CTGGAG 2 cut(s) 62, 1347
HaeIII GGCC 4 cut(s) 206, 353, 1074, 1273
HapII CCGG 1 cut(s) 932
HgaI GACGC 2 cut(s) 359, 1453
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 4 cut(s) 14, 215, 329, 1370
HpaII CCGG 1 cut(s) 932
HphI GGTGA 4 cut(s) 314, 332, 587, 1240
Hpy166II GTNNAC 2 cut(s) 697, 1552
Hpy188I TCNGA 4 cut(s) 19, 396, 813, 1111
Hpy188III TCNNGA 5 cut(s) 199, 447, 779, 833, 845
Hpy8I GTNNAC 2 cut(s) 697, 1552
HpyAV CCTTC 4 cut(s) 444, 730, 1017, 1219
HpyCH4III ACNGT 2 cut(s) 724, 1377
HpyCH4IV ACGT 2 cut(s) 442, 1538
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 431
HpyF3I CTNAG 4 cut(s) 421, 691, 789, 1156
HpySE526I ACGT 2 cut(s) 442, 1538
KpnI GGTACC 1 cut(s) 1341
Ksp22I TGATCA 2 cut(s) 808, 1399
Kzo9I GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
LmnI GCTCC 2 cut(s) 1083, 1219
Lsp1109I GCAGC 5 cut(s) 169, 367, 1300, 1322, 1428
LweI GCATC 6 cut(s) 137, 394, 901, 1095, 1297, 1522
MaeI CTAG 5 cut(s) 342, 588, 1317, 1382, 1433
MaeII ACGT 2 cut(s) 442, 1538
MaeIII GTNAC 2 cut(s) 1178, 1393
MalI GATC 7 cut(s) 118, 606, 810, 840, 895, 1401, 1458
MboI GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
MboII GAAGA 8 cut(s) 38, 86, 89, 224, 483, 886, 1124, 1400
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 1 cut(s) 893
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 7 cut(s) 64, 98, 152, 505, 745, 962, 1115
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 2 cut(s) 8, 1364
MmeI TCCRAC 2 cut(s) 523, 979
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 206
MseI TTAA 8 cut(s) 239, 621, 863, 903, 1043, 1118, 1260, 1556
MslI CAYNNNNRTG 2 cut(s) 133, 479
Msp20I TGGCCA 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 166, 1313
MspCI CTTAAG 1 cut(s) 1555
MspI CCGG 1 cut(s) 932
MspR9I CCNGG 3 cut(s) 71, 933, 1326
MunI CAATTG 1 cut(s) 98
MvaI CCWGG 2 cut(s) 71, 1326
MwoI GCNNNNNNNGC 2 cut(s) 103, 431
NciI CCSGG 1 cut(s) 933
NdeII GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
NlaIV GGNNCC 3 cut(s) 352, 895, 1339
NmeAIII GCCGAG 2 cut(s) 303, 940
NmuCI GTSAC 1 cut(s) 1393
NspI RCATGY 4 cut(s) 713, 862, 923, 1280
PagI TCATGA 2 cut(s) 778, 844
PciI ACATGT 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 1006
PfeI GAWTC 2 cut(s) 215, 329
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 6 cut(s) 184, 382, 1275, 1312, 1315, 1443
PleI GAGTC 2 cut(s) 8, 1364
PpsI GAGTC 2 cut(s) 8, 1364
PscI ACATGT 1 cut(s) 709
Psp6I CCWGG 2 cut(s) 69, 1324
PspGI CCWGG 2 cut(s) 69, 1324
PspN4I GGNNCC 3 cut(s) 352, 895, 1339
PspPI GGNCC 3 cut(s) 196, 351, 1072
PstI CTGCAG 1 cut(s) 1210
PsuI RGATCY 1 cut(s) 893
PvuII CAGCTG 1 cut(s) 1313
RsaI GTAC 5 cut(s) 752, 930, 1154, 1339, 1357
RsaNI GTAC 5 cut(s) 751, 929, 1153, 1338, 1356
RseI CAYNNNNRTG 2 cut(s) 133, 479
SaqAI TTAA 8 cut(s) 239, 621, 863, 903, 1043, 1118, 1260, 1556
SatI GCNGC 6 cut(s) 183, 381, 1274, 1311, 1314, 1442
Sau3AI GATC 7 cut(s) 116, 604, 808, 838, 893, 1399, 1456
Sau96I GGNCC 3 cut(s) 196, 351, 1072
SchI GAGTC 2 cut(s) 8, 1364
ScrFI CCNGG 3 cut(s) 71, 933, 1326
SfaNI GCATC 6 cut(s) 137, 394, 901, 1095, 1297, 1522
SfcI CTRYAG 1 cut(s) 1206
SinI GGWCC 1 cut(s) 196
SmiMI CAYNNNNRTG 2 cut(s) 133, 479
SmlI CTYRAG 2 cut(s) 833, 1555
SmoI CTYRAG 2 cut(s) 833, 1555
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 7 cut(s) 64, 98, 152, 505, 745, 962, 1115
SsiI CCGC 4 cut(s) 166, 1274, 1303, 1366
SspI AATATT 1 cut(s) 190
SspMI CTAG 5 cut(s) 342, 588, 1317, 1382, 1433
StyD4I CCNGG 3 cut(s) 69, 931, 1324
TaaI ACNGT 2 cut(s) 724, 1377
TaiI ACGT 2 cut(s) 445, 1541
TaqI TCGA 2 cut(s) 1386, 1501
TasI AATT 7 cut(s) 64, 98, 152, 505, 745, 962, 1115
TatI WGTACW 1 cut(s) 1355
TauI GCSGC 1 cut(s) 1276
TfiI GAWTC 2 cut(s) 215, 329
Tru1I TTAA 8 cut(s) 239, 621, 863, 903, 1043, 1118, 1260, 1556
Tru9I TTAA 8 cut(s) 239, 621, 863, 903, 1043, 1118, 1260, 1556
TscAI CASTG 5 cut(s) 441, 532, 733, 1194, 1402
TseFI GTSAC 1 cut(s) 1393
TseI GCWGC 5 cut(s) 182, 380, 1310, 1313, 1441
Tsp45I GTSAC 1 cut(s) 1393
TspDTI ATGAA 9 cut(s) 50, 218, 225, 281, 485, 980, 1121, 1400, 1464
TspGWI ACGGA 2 cut(s) 1237, 1276
TspRI CASTG 5 cut(s) 441, 532, 733, 1194, 1402
Van91I CCANNNNNTGG 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 1555
VpaK11BI GGWCC 1 cut(s) 196
XceI RCATGY 4 cut(s) 713, 862, 923, 1280
XmiI GTMKAC 1 cut(s) 1551
XmnI GAANNNNTTC 1 cut(s) 1006
XspI CTAG 5 cut(s) 342, 588, 1317, 1382, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.