RLG00000024453

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002212
Physical Location & Seq
Forward (+)
35965759 .. 35967081
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024453

Sequence Viewer

Length: 1323 bp
ATGGAGGCCTTTGACTCTGTGATTGAAGAAGCAAGGAAACCGGAGAGTAGGGTTGAACAAAGTCACGCCCTGGAAGCAGAAAGCATGTTGGCCAAGCAATTGAAGCATGAGGAGAGTTCAATGGAAGCATCATCATGTCAAATGGCTACTCGATGGAAATGTCATCAGAGGGAAATAATTGCTGCACATATTTTGGTACAGATGGCTAATGAAGATTCTGGTATGCTTTGTAACGGGTTAAATTCACATGGAGTGAAGCATAGCATGGAAGGGATGCTGAGAATGAGGTGGGAAACGAGAGGTGGTAGAATAGCAGGTGATAGTCATTCTGAAGCGGATGGGAGAAAGGCCAAGTTTCTGGCGAAGGGCCAGCAAGCTGCCTTGGGAGGTGAGATGAATGATGGAGATAGTGTAGTCTTGGCTTTATCAGCAATGCCATGTCCTGGAAGGAGAAAGCATAGAAAGATGAACATCGGTATGGAATTCTCTTCTCTTCGACCAAAGTACATGAACTGTAATGTCAGTGAGGACAAGGATGACAATTTGTCTGAAGAGTGTGGAGTTTGTGATGGTGATGAGCTGACTAGTAGATGGGGAAACAATAATCAGAAATCGATTGAAGCGGGTCTAGAGGATCGTCATGATAGATTAGGCATGTCCAGGATAAAGAAGCGGAAAACAGTGGCAATATCCTGCTTTAGTAGAGAAACTGAGGATGGTCAAGAGAGATTTCTTCATGCTGGGAGCCATCCTGCTGCACCACCTCTGGTTTTGATGGAACCAACATTAAACAAAAGGGAGAGGTTTAGGGGTATGAAACGTAAGATGAACATCGCCACTATCAGCCCAAGCTCTGAAGAAGTAAGAGCTCACTTTCGCAAGGAGGAAGCATTGAGGTATTTAGTCTCGGATAGGGGATTTTGTTACACTGCTCTGGATGGTAGAAAGTCTGCAGTCGCTCCTCTAACAAAGATGAAAAAGATTGCCCGATATCATGTTATACTCAAACCTGATAGGCCGCGTCATATTTACACCCTGTGCCTGGTGCGGGATGCAGCTGCTAGATTGCCTGGAGGTGTGGGCACCAGAGCAGATATTTGTACACTCATGCGCGACTCACAGTATCTAGTTGAAGACATCTCCGATGAACAACTTAATCAAGTTGCTAGCAAAGGACTGGACAGGCTGCACTATGAAGTTGATCCCTGCGTCAAGTATGATGGGAAGTTGTGGGTTTATTTACATCGAAATAGAGGGGAGGACGACTTTAAGGACGATGCTACATCATCTGCCTATGTAAGAAGAAGGTACCGCGGAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

441

Amino Acids

49.78

Weight (kDa)

8.71

Isoelectric Point (pI)

58.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 289 - 421 1.6e-52 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 1308
AccB1I GGYRCC 2 cut(s) 1082, 1308
AccB7I CCANNNNNTGG 1 cut(s) 443
AccII CGCG 3 cut(s) 1021, 1113, 1314
AciI CCGC 7 cut(s) 335, 623, 673, 1019, 1048, 1312, 1314
AclWI GGATC 2 cut(s) 642, 1196
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 2 cut(s) 241, 482
AcuI CTGAAG 3 cut(s) 351, 570, 876
AdeI CACNNNGTG 1 cut(s) 1038
AfaI GTAC 4 cut(s) 198, 506, 1102, 1310
AfiI CCNNNNNNNGG 3 cut(s) 443, 1042, 1048
AgsI TTSAA 6 cut(s) 26, 56, 103, 120, 620, 1133
AhdI GACNNNNNGTC 1 cut(s) 544
AhlI ACTAGT 1 cut(s) 584
AjnI CCWGG 5 cut(s) 69, 442, 659, 1041, 1069
AluBI AGCT 5 cut(s) 377, 580, 852, 869, 1058
AluI AGCT 5 cut(s) 377, 580, 852, 869, 1058
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 1 cut(s) 910
AlwI GGATC 2 cut(s) 642, 1196
AoxI GGCC 5 cut(s) 6, 90, 348, 367, 1016
ApeKI GCWGC 6 cut(s) 182, 377, 755, 1055, 1058, 1186
ApoI RAATTY 2 cut(s) 241, 482
Asp718I GGTACC 1 cut(s) 1308
AspLEI GCGC 1 cut(s) 1113
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 3 cut(s) 329, 401, 584
AsuNHI GCTAGC 1 cut(s) 1166
BaeGI GKGCMC 1 cut(s) 1085
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 2 cut(s) 1082, 1308
BanII GRGCYC 1 cut(s) 871
BbsI GAAGAC 1 cut(s) 1140
Bbv12I GWGCWC 1 cut(s) 871
BbvI GCAGC 6 cut(s) 169, 364, 742, 1045, 1067, 1173
BciT130I CCWGG 5 cut(s) 71, 444, 661, 1043, 1071
BcoDI GTCTC 1 cut(s) 910
BcuI ACTAGT 1 cut(s) 584
BfaI CTAG 5 cut(s) 585, 629, 1062, 1127, 1167
BfmI CTRYAG 1 cut(s) 951
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 7 cut(s) 183, 378, 756, 1019, 1056, 1059, 1187
BlsI GCNGC 7 cut(s) 184, 379, 757, 1020, 1057, 1060, 1188
Bme1390I CCNGG 5 cut(s) 71, 444, 661, 1043, 1071
BmeRI GACNNNNNGTC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 4 cut(s) 746, 780, 1084, 1310
BmrFI CCNGG 5 cut(s) 71, 444, 661, 1043, 1071
BmsI GCATC 4 cut(s) 137, 264, 1042, 1267
BmtI GCTAGC 1 cut(s) 1170
BpiI GAAGAC 1 cut(s) 1140
BpmI CTGGAG 1 cut(s) 1092
Bsa29I ATCGAT 1 cut(s) 614
BsaBI GATNNNNATC 2 cut(s) 470, 830
BsaJI CCNNGG 3 cut(s) 69, 381, 1312
BsaWI WCCGGW 1 cut(s) 40
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 1042, 1048
Bse1I ACTGG 1 cut(s) 1182
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 2 cut(s) 470, 830
BseBI CCWGG 5 cut(s) 71, 444, 661, 1043, 1071
BseCI ATCGAT 1 cut(s) 614
BseDI CCNNGG 3 cut(s) 69, 381, 1312
BseGI GGATG 7 cut(s) 279, 343, 541, 721, 748, 943, 1057
BseJI GATNNNNATC 2 cut(s) 470, 830
BseLI CCNNNNNNNGG 3 cut(s) 443, 1042, 1048
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 2 cut(s) 269, 702
BseNI ACTGG 1 cut(s) 1182
BseRI GAGGAG 2 cut(s) 125, 951
BseSI GKGCMC 1 cut(s) 1085
BseXI GCAGC 6 cut(s) 169, 364, 742, 1045, 1067, 1173
BseYI CCCAGC 1 cut(s) 740
BsgI GTGCAG 3 cut(s) 168, 741, 1172
Bsh1236I CGCG 3 cut(s) 1021, 1113, 1314
BshFI GGCC 5 cut(s) 8, 92, 350, 369, 1018
BshNI GGYRCC 2 cut(s) 1082, 1308
BshVI ATCGAT 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 871
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 3 cut(s) 443, 1042, 1048
BsmAI GTCTC 1 cut(s) 910
BsnI GGCC 5 cut(s) 8, 92, 350, 369, 1018
Bsp1286I GDGCHC 2 cut(s) 871, 1085
Bsp1407I TGTACA 1 cut(s) 1100
Bsp143I GATC 2 cut(s) 634, 1201
BspACI CCGC 7 cut(s) 335, 623, 673, 1019, 1048, 1312, 1314
BspANI GGCC 5 cut(s) 8, 92, 350, 369, 1018
BspCNI CTCAG 2 cut(s) 270, 703
BspDI ATCGAT 1 cut(s) 614
BspFNI CGCG 3 cut(s) 1021, 1113, 1314
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 4 cut(s) 746, 780, 1084, 1310
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 1 cut(s) 305
BspOI GCTAGC 1 cut(s) 1170
BspPI GGATC 2 cut(s) 642, 1196
BspT107I GGYRCC 2 cut(s) 1082, 1308
BsrDI GCAATG 1 cut(s) 438
BsrGI TGTACA 1 cut(s) 1100
BsrI ACTGG 1 cut(s) 1182
BssECI CCNNGG 3 cut(s) 69, 381, 1312
BssMI GATC 2 cut(s) 634, 1201
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 5 cut(s) 71, 444, 661, 1043, 1071
Bst4CI ACNGT 3 cut(s) 515, 682, 1122
Bst6I CTCTTC 3 cut(s) 493, 498, 546
BstAUI TGTACA 1 cut(s) 1100
BstC8I GCNNGC 3 cut(s) 371, 375, 1168
BstDEI CTNAG 2 cut(s) 278, 711
BstDSI CCRYGG 1 cut(s) 1312
BstF5I GGATG 7 cut(s) 279, 343, 541, 721, 748, 943, 1057
BstFNI CGCG 3 cut(s) 1021, 1113, 1314
BstHHI GCGC 1 cut(s) 1113
BstKTI GATC 2 cut(s) 637, 1204
BstMAI GTCTC 1 cut(s) 910
BstMBI GATC 2 cut(s) 634, 1201
BstMWI GCNNNNNNNGC 3 cut(s) 74, 103, 428
BstNI CCWGG 5 cut(s) 71, 444, 661, 1043, 1071
BstNSI RCATGY 2 cut(s) 88, 658
BstSCI CCNGG 5 cut(s) 69, 442, 659, 1041, 1069
BstSFI CTRYAG 1 cut(s) 951
BstSLI GKGCMC 1 cut(s) 1085
BstUI CGCG 3 cut(s) 1021, 1113, 1314
BstV1I GCAGC 6 cut(s) 169, 364, 742, 1045, 1067, 1173
BstV2I GAAGAC 1 cut(s) 1140
BstXI CCANNNNNNTGG 1 cut(s) 358
Bsu15I ATCGAT 1 cut(s) 614
BsuRI GGCC 5 cut(s) 8, 92, 350, 369, 1018
BsuTUI ATCGAT 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 1312
BtgZI GCGATG 1 cut(s) 817
BtsCI GGATG 7 cut(s) 279, 343, 541, 721, 748, 943, 1057
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 529, 687, 927
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 3 cut(s) 371, 375, 1168
CciI TCATGA 1 cut(s) 640
CfoI GCGC 1 cut(s) 1113
Cfr13I GGNCC 1 cut(s) 367
Cfr42I CCGCGG 1 cut(s) 1315
ClaI ATCGAT 1 cut(s) 614
CseI GACGC 2 cut(s) 1010, 1198
Csp6I GTAC 4 cut(s) 197, 505, 1101, 1309
CviQI GTAC 4 cut(s) 197, 505, 1101, 1309
DdeI CTNAG 2 cut(s) 278, 711
DpnI GATC 2 cut(s) 636, 1203
DpnII GATC 2 cut(s) 634, 1201
DraIII CACNNNGTG 1 cut(s) 1038
DriI GACNNNNNGTC 1 cut(s) 544
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 3 cut(s) 493, 498, 546
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 3 cut(s) 493, 498, 546
Ecl136II GAGCTC 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 1 cut(s) 871
Eco32I GATATC 1 cut(s) 992
Eco53kI GAGCTC 1 cut(s) 869
Eco57I CTGAAG 3 cut(s) 351, 570, 876
EcoICRI GAGCTC 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 5 cut(s) 69, 442, 659, 1041, 1069
EcoRV GATATC 1 cut(s) 992
EcoT14I CCWWGG 1 cut(s) 381
EcoT38I GRGCYC 1 cut(s) 871
ErhI CCWWGG 1 cut(s) 381
FauI CCCGC 2 cut(s) 616, 1041
Fnu4HI GCNGC 7 cut(s) 183, 378, 756, 1019, 1056, 1059, 1187
FokI GGATG 7 cut(s) 286, 350, 548, 728, 735, 950, 1064
FriOI GRGCYC 1 cut(s) 871
Fsp4HI GCNGC 7 cut(s) 183, 378, 756, 1019, 1056, 1059, 1187
FspBI CTAG 5 cut(s) 585, 629, 1062, 1127, 1167
GlaI GCGC 1 cut(s) 1112
GluI GCNGC 7 cut(s) 183, 378, 756, 1019, 1056, 1059, 1187
GsaI CCCAGC 1 cut(s) 744
GsuI CTGGAG 1 cut(s) 1092
HaeIII GGCC 5 cut(s) 8, 92, 350, 369, 1018
HapII CCGG 1 cut(s) 41
HgaI GACGC 2 cut(s) 1010, 1198
HhaI GCGC 1 cut(s) 1113
Hin6I GCGC 1 cut(s) 1111
HinP1I GCGC 1 cut(s) 1111
HinfI GANTC 3 cut(s) 14, 215, 1115
HpaII CCGG 1 cut(s) 41
HphI GGTGA 3 cut(s) 329, 401, 584
Hpy166II GTNNAC 1 cut(s) 1103
Hpy188I TCNGA 7 cut(s) 168, 331, 550, 609, 856, 910, 1144
Hpy188III TCNNGA 4 cut(s) 629, 641, 722, 935
Hpy8I GTNNAC 1 cut(s) 1103
HpyAV CCTTC 4 cut(s) 263, 358, 441, 1299
HpyCH4III ACNGT 3 cut(s) 515, 682, 1122
HpyCH4IV ACGT 1 cut(s) 820
HpyCH4V TGCA 5 cut(s) 185, 758, 953, 1055, 1189
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 103, 428
HpyF3I CTNAG 2 cut(s) 278, 711
HpySE526I ACGT 1 cut(s) 820
HspAI GCGC 1 cut(s) 1111
KpnI GGTACC 1 cut(s) 1312
KspI CCGCGG 1 cut(s) 1315
Kzo9I GATC 2 cut(s) 634, 1201
LmnI GCTCC 2 cut(s) 744, 964
Lsp1109I GCAGC 6 cut(s) 169, 364, 742, 1045, 1067, 1173
LweI GCATC 4 cut(s) 137, 264, 1042, 1267
MaeI CTAG 5 cut(s) 585, 629, 1062, 1127, 1167
MaeII ACGT 1 cut(s) 820
MaeIII GTNAC 3 cut(s) 62, 230, 923
MalI GATC 2 cut(s) 636, 1203
MboI GATC 2 cut(s) 634, 1201
MboII GAAGA 9 cut(s) 38, 224, 480, 485, 563, 725, 869, 1145, 1314
MfeI CAATTG 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 871, 1085
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 5 cut(s) 98, 177, 241, 482, 541
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 2 cut(s) 8, 1109
Mox20I TGGCCA 1 cut(s) 92
MscI TGGCCA 1 cut(s) 92
MseI TTAA 4 cut(s) 239, 788, 1155, 1269
MslI CAYNNNNRTG 2 cut(s) 133, 476
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 2 cut(s) 1058, 1314
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 5 cut(s) 71, 444, 661, 1043, 1071
MunI CAATTG 1 cut(s) 98
MvaI CCWGG 5 cut(s) 71, 444, 661, 1043, 1071
MvnI CGCG 3 cut(s) 1021, 1113, 1314
MwoI GCNNNNNNNGC 3 cut(s) 74, 103, 428
NdeII GATC 2 cut(s) 634, 1201
NheI GCTAGC 1 cut(s) 1166
NlaIV GGNNCC 4 cut(s) 746, 780, 1084, 1310
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 2 cut(s) 88, 658
PagI TCATGA 1 cut(s) 640
PaqCI CACCTGC 1 cut(s) 305
PceI AGGCCT 1 cut(s) 8
PfeI GAWTC 1 cut(s) 215
PflMI CCANNNNNTGG 1 cut(s) 443
PfoI TCCNGGA 2 cut(s) 442, 659
PkrI GCNGC 7 cut(s) 184, 379, 757, 1020, 1057, 1060, 1188
PleI GAGTC 2 cut(s) 8, 1109
PpsI GAGTC 2 cut(s) 8, 1109
Psp124BI GAGCTC 1 cut(s) 871
Psp6I CCWGG 5 cut(s) 69, 442, 659, 1041, 1069
PspFI CCCAGC 1 cut(s) 740
PspGI CCWGG 5 cut(s) 69, 442, 659, 1041, 1069
PspN4I GGNNCC 4 cut(s) 746, 780, 1084, 1310
PspPI GGNCC 1 cut(s) 367
PstI CTGCAG 1 cut(s) 955
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 4 cut(s) 198, 506, 1102, 1310
RsaNI GTAC 4 cut(s) 197, 505, 1101, 1309
RseI CAYNNNNRTG 2 cut(s) 133, 476
SacI GAGCTC 1 cut(s) 871
SacII CCGCGG 1 cut(s) 1315
SaqAI TTAA 4 cut(s) 239, 788, 1155, 1269
SatI GCNGC 7 cut(s) 183, 378, 756, 1019, 1056, 1059, 1187
Sau3AI GATC 2 cut(s) 634, 1201
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 2 cut(s) 8, 1109
ScrFI CCNGG 5 cut(s) 71, 444, 661, 1043, 1071
SduI GDGCHC 2 cut(s) 871, 1085
SfaNI GCATC 4 cut(s) 137, 264, 1042, 1267
SfcI CTRYAG 1 cut(s) 951
Sfr303I CCGCGG 1 cut(s) 1315
SgrBI CCGCGG 1 cut(s) 1315
SmiMI CAYNNNNRTG 2 cut(s) 133, 476
SpeI ACTAGT 1 cut(s) 584
Sse9I AATT 5 cut(s) 98, 177, 241, 482, 541
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 7 cut(s) 335, 623, 673, 1019, 1048, 1312, 1314
SspMI CTAG 5 cut(s) 585, 629, 1062, 1127, 1167
SstI GAGCTC 1 cut(s) 871
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 5 cut(s) 69, 442, 659, 1041, 1069
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 3 cut(s) 515, 682, 1122
TaiI ACGT 1 cut(s) 823
TaqI TCGA 4 cut(s) 151, 496, 614, 1246
TasI AATT 5 cut(s) 98, 177, 241, 482, 541
TatI WGTACW 2 cut(s) 504, 1100
TauI GCSGC 1 cut(s) 1021
TfiI GAWTC 1 cut(s) 215
Tru1I TTAA 4 cut(s) 239, 788, 1155, 1269
Tru9I TTAA 4 cut(s) 239, 788, 1155, 1269
TscAI CASTG 3 cut(s) 529, 687, 934
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 6 cut(s) 182, 377, 755, 1055, 1058, 1186
Tsp45I GTSAC 1 cut(s) 62
TspRI CASTG 3 cut(s) 529, 687, 934
Van91I CCANNNNNTGG 1 cut(s) 443
XapI RAATTY 2 cut(s) 241, 482
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 2 cut(s) 88, 658
XspI CTAG 5 cut(s) 585, 629, 1062, 1127, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.