Rh3AG140800

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0002212
Physical Location & Seq
Reverse (-)
12365983 .. 12369840
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG140800.1

Sequence Viewer

Length: 765 bp
ATGATTCAAAAGGGGCTAGGAGAGGGGGCCAAGTTTATTGCAAAGCGTCAGCAAGCTGCGTTTGGAGGTCAGACGAATGATGCAGATAGTGTAGTCTTAGCTTTATCAGCAGTGCTATGTTCTGGAAGGAGAAAGAATAGACAGATGAACATCGGTATGAAACTGTCTTCAATCCAACCAAATTATATAAATACTAGTGTCAGTGATAACAAGGATAACAATTTATGTGAAGCGTGTGGAGTTTTGGATGGTGATGAGCTAACTAGTAGATGGGGAAACGATCATCGCAAGTCATTTAAAGCTTGTGTTGAGGGTTGTCATGATAGATTAGTTCCTATTGTTGAGAGAGTTCATCCAGTTGGAAGCCCTACTGCACCACCTCTGGTTTTGATGAGACCAACATTAAACAAAAGGAAAAGGTTTAGGGGTGTGGGCCGTAGGAGGAGCATCGCAACTATCAGCCCAAGCTCTGAAAAAGTAAGAGCTTACTTTCATAACGAGGAAGCTTTGAGGTACTTAGTCCCTGATAGGGGATTTTGTTACACAGCACTGGATGGTATAAAGTCTGCAGTCGCTCCTCTACGAAAGGTGACCAACATACACCGAGACCATGTTATGCTTAAAGCTGATAGGCCGCATGTTGTTTCTGTCTGCTGTCTTGTGCGGGATGCAGCTACTAGATTGCCTGGAGCCCAAAGATTCTTGGAGTGGCTATCTAGGGATACAATTTTGGAGGTCGTCGTGCCTTGGTGGATTATGATGTAG

Protein Analysis

254

Amino Acids

28.39

Weight (kDa)

9.8

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 161 - 231 6.8e-20 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 635, 664
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 529, 530
AgsI TTSAA 2 cut(s) 8, 171
AhlI ACTAGT 2 cut(s) 194, 263
AjnI CCWGG 1 cut(s) 685
AluBI AGCT 9 cut(s) 56, 101, 259, 302, 468, 485, 506, 626, 674
AluI AGCT 9 cut(s) 56, 101, 259, 302, 468, 485, 506, 626, 674
Alw26I GTCTC 2 cut(s) 388, 600
AoxI GGCC 3 cut(s) 27, 433, 632
ApeKI GCWGC 2 cut(s) 56, 671
AspS9I GGNCC 2 cut(s) 27, 433
AsuHPI GGTGA 2 cut(s) 263, 601
BanII GRGCYC 1 cut(s) 694
BbsI GAAGAC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 43, 683
BccI CCATC 3 cut(s) 242, 264, 548
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 1 cut(s) 687
BciVI GTATCC 1 cut(s) 715
BcoDI GTCTC 2 cut(s) 388, 600
BcuI ACTAGT 2 cut(s) 194, 263
BfaI CTAG 5 cut(s) 17, 195, 264, 678, 717
BfmI CTRYAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 715
BisI GCNGC 3 cut(s) 57, 635, 672
BlsI GCNGC 3 cut(s) 58, 636, 673
Bme1390I CCNGG 1 cut(s) 687
BmgT120I GGNCC 2 cut(s) 27, 433
BmiI GGNNCC 2 cut(s) 28, 691
BmrFI CCNGG 1 cut(s) 687
BmsI GCATC 3 cut(s) 70, 456, 658
BpiI GAAGAC 1 cut(s) 159
BpmI CTGGAG 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 149
BsaI GGTCTC 2 cut(s) 388, 600
BsaJI CCNNGG 1 cut(s) 746
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 530
Bse1I ACTGG 2 cut(s) 356, 555
Bse8I GATNNNNATC 1 cut(s) 149
BseBI CCWGG 1 cut(s) 687
BseDI CCNNGG 1 cut(s) 746
BseGI GGATG 4 cut(s) 253, 352, 559, 673
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 529, 530
BseNI ACTGG 2 cut(s) 356, 555
BseRI GAGGAG 2 cut(s) 457, 567
BseXI GCAGC 2 cut(s) 43, 683
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 3 cut(s) 29, 435, 634
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 2 cut(s) 529, 530
BsmAI GTCTC 2 cut(s) 388, 600
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 3 cut(s) 29, 435, 634
Bso31I GGTCTC 2 cut(s) 388, 600
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 1 cut(s) 280
BspACI CCGC 2 cut(s) 635, 664
BspANI GGCC 3 cut(s) 29, 435, 634
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 28, 691
BspMAI CTGCAG 1 cut(s) 571
BspTNI GGTCTC 2 cut(s) 388, 600
BsrI ACTGG 2 cut(s) 356, 555
BssECI CCNNGG 1 cut(s) 746
BssMI GATC 1 cut(s) 280
BssT1I CCWWGG 1 cut(s) 746
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 2 cut(s) 97, 517
BstEII GGTNACC 1 cut(s) 589
BstF5I GGATG 4 cut(s) 253, 352, 559, 673
BstKTI GATC 1 cut(s) 283
BstMAI GTCTC 2 cut(s) 388, 600
BstMBI GATC 1 cut(s) 280
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 1 cut(s) 687
BstNSI RCATGY 1 cut(s) 641
BstPI GGTNACC 1 cut(s) 589
BstSCI CCNGG 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 43, 683
BstV2I GAAGAC 1 cut(s) 159
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 3 cut(s) 29, 435, 634
BtgZI GCGATG 2 cut(s) 269, 433
BtsCI GGATG 4 cut(s) 253, 352, 559, 673
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 117, 208, 548
Cac8I GCNNGC 1 cut(s) 54
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 27, 433
CseI GACGC 1 cut(s) 35
Csp6I GTAC 1 cut(s) 514
CviAII CATG 3 cut(s) 320, 611, 638
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 2 cut(s) 97, 517
DpnI GATC 1 cut(s) 282
DpnII GATC 1 cut(s) 280
DraI TTTAAA 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 746
Eco24I GRGCYC 1 cut(s) 694
Eco31I GGTCTC 2 cut(s) 388, 600
Eco91I GGTNACC 1 cut(s) 589
EcoO65I GGTNACC 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 746
EcoT38I GRGCYC 1 cut(s) 694
ErhI CCWWGG 1 cut(s) 746
FaeI CATG 3 cut(s) 323, 614, 641
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 319, 610, 637
FauI CCCGC 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 57, 635, 672
FokI GGATG 4 cut(s) 260, 339, 566, 680
FriOI GRGCYC 1 cut(s) 694
Fsp4HI GCNGC 3 cut(s) 57, 635, 672
FspBI CTAG 5 cut(s) 17, 195, 264, 678, 717
GluI GCNGC 3 cut(s) 57, 635, 672
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 3 cut(s) 29, 435, 634
HgaI GACGC 1 cut(s) 35
Hin1II CATG 3 cut(s) 323, 614, 641
HindIII AAGCTT 2 cut(s) 300, 504
HinfI GANTC 2 cut(s) 4, 699
HphI GGTGA 2 cut(s) 263, 601
Hpy188I TCNGA 2 cut(s) 72, 472
Hpy188III TCNNGA 2 cut(s) 123, 320
Hpy99I CGWCG 1 cut(s) 743
HpyAV CCTTC 1 cut(s) 120
HpyCH4III ACNGT 1 cut(s) 165
HpyCH4V TGCA 5 cut(s) 41, 83, 374, 569, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 97, 517
Hsp92II CATG 3 cut(s) 323, 614, 641
Kzo9I GATC 1 cut(s) 280
LmnI GCTCC 3 cut(s) 444, 580, 689
LpnPI CCDG 7 cut(s) 108, 368, 369, 536, 537, 672, 699
Lsp1109I GCAGC 2 cut(s) 43, 683
LweI GCATC 3 cut(s) 70, 456, 658
MaeI CTAG 5 cut(s) 17, 195, 264, 678, 717
MaeIII GTNAC 2 cut(s) 539, 589
MalI GATC 1 cut(s) 282
MboI GATC 1 cut(s) 280
MboII GAAGA 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 3 cut(s) 181, 220, 726
MmeI TCCRAC 2 cut(s) 199, 340
MnlI CCTC 9 cut(s) 16, 59, 304, 390, 435, 493, 504, 588, 727
MseI TTAA 3 cut(s) 297, 404, 621
MslI CAYNNNNRTG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 687
MvaI CCWGG 1 cut(s) 687
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 1 cut(s) 280
NlaIII CATG 3 cut(s) 323, 614, 641
NlaIV GGNNCC 2 cut(s) 28, 691
NmuCI GTSAC 1 cut(s) 589
NspI RCATGY 1 cut(s) 641
PagI TCATGA 1 cut(s) 319
PfeI GAWTC 2 cut(s) 4, 699
PkrI GCNGC 3 cut(s) 58, 636, 673
Psp6I CCWGG 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 589
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 28, 691
PspPI GGNCC 2 cut(s) 27, 433
PstI CTGCAG 1 cut(s) 571
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 3 cut(s) 297, 404, 621
SatI GCNGC 3 cut(s) 57, 635, 672
Sau3AI GATC 1 cut(s) 280
Sau96I GGNCC 2 cut(s) 27, 433
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 1 cut(s) 694
SfaNI GCATC 3 cut(s) 70, 456, 658
SfcI CTRYAG 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 155
SpeI ACTAGT 2 cut(s) 194, 263
Sse9I AATT 3 cut(s) 181, 220, 726
SsiI CCGC 2 cut(s) 635, 664
SspMI CTAG 5 cut(s) 17, 195, 264, 678, 717
StyD4I CCNGG 1 cut(s) 685
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 1 cut(s) 165
TasI AATT 3 cut(s) 181, 220, 726
TauI GCSGC 1 cut(s) 637
TfiI GAWTC 2 cut(s) 4, 699
Tru1I TTAA 3 cut(s) 297, 404, 621
Tru9I TTAA 3 cut(s) 297, 404, 621
TscAI CASTG 3 cut(s) 117, 208, 555
TseFI GTSAC 1 cut(s) 589
TseI GCWGC 2 cut(s) 56, 671
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 4 cut(s) 161, 173, 341, 482
TspRI CASTG 3 cut(s) 117, 208, 555
XceI RCATGY 1 cut(s) 641
XspI CTAG 5 cut(s) 17, 195, 264, 678, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.