Rw3G013300

protease binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002212
Physical Location & Seq
Reverse (-)
13166701 .. 13168281
1581 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013300.1

Sequence Viewer

Length: 1455 bp
ATGGTGTCAACTCGACGATTGTACTGTGCTGATTTGCTGTTAGGACCTAGGATATTGGAGAGTAAGGTAGAGAAAAGTAATTGCCTGGAAGAAGAAATCATGTTAGCAGAGCAATTAAAGCATGAGGAGAGATCAATGGAAGCATCATCATGTCAAATGGCTATGGAATTGAAATGTCAGCAGAGAGATATAGTTGCTGCAAATATTTTGGTCCAAATGGCCAATGAAGATTCTGCTTTGCTTCATCACAGATTAAGTTTGCATGGAGTGAAGCGTAGCATGAAAGGGAAGCCGAGAATGAGGTGGGAAACGAAAGGTGATAGAATAGGAGTGAGTGATAATGATTCCAAAGGGGCTAGGAGAGGCGCCAAGTTTATTGCAAAGTGTCAGCAAGCCGCGTTTGGAGGTCAGACGAATGATGCAGATAGTGTAGTCTTAGCTTTATCAGCAGTGCTACGTTCTAGAAGGAGAAAGAATAGAAAGATGATCATCGGTATGAAACTGTCTTCAATCCAACCAAATTATATAAATACTAATGTCAGTGATAACAAGGATGACAATTTATGTGAAGCGTGTGGAGTTTTGGATGGTGATGAGCTAACTAGTAGATGGGGAAACGATCATCGCAAGTCATTTAAAGCTTGTAAAAAAAATGTGGATGTTGCAGGCTCAGTAAACATACCAAGACATGTGAAATATACTGTCAGTGAGGAGGAGGACGATAATTTGTATGAGGCACGACAGTTTTTCGATGTTGAAGCTGGTCTTGAGGACTGTCATGATAGATTAGGCATGTTAAGTATAAAGAAGAGGAAAAGAGTGGCAGGATCCTACTTTAAAAAAGATGCCGAGGATGTTGCAGTACCAGGGAAAATACCATTGAAAATGCCCTTGAAATTAGCAGTTCCTATTGTTGAGAGACTTCATCCAGTTGGAAGCCAGACTGCACCACCTCTGGTTTTGATGAGACCAACATTAAACAAAAGGAAAAGGTTTAGGGGTGTGGGCCGTAAGAGGAGCATCGCAACTATTAGCCCAAGCTCTGAAGAAGTAAGAGCTTACTTTCATAACGAGGAAGCTTTGAGGTATTTAGTCCCTGATAGGGAATTTTGTTACACAGCACTGGATGGTAGAAAGTTTACAGTCGCTCCTCTACGGAAGGTGAGCAAAATACACCGAGACCATGTTATGCTTAAAGTTGATAGGCCGCGTGTTGTTTCTGTCTGCTATCTTGTGCGGGATGCAGCTGCTAGATTGCTTGGAGGTATGGGTAGCAGAACAGATATATGTACTCTCATGCGGGACTCACACAAAGGACTAGACAGGCTGCACTATGAAGTTGATCCCTGCGTCAAGTATGATGGGAAGCTGTGGGTTTATTTACATCGAAATAGAGGGGAGGAGGACTTTGAGGATGATGCTACGTTATGTTCTATCTACTTAAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

55.03

Weight (kDa)

9.38

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_2nd_NFRKB PF25793 352 - 468 1.9e-34 NFRKB second winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 2 cut(s) 398, 1210
AciI CCGC 4 cut(s) 396, 1208, 1237, 1300
AclWI GGATC 3 cut(s) 822, 835, 1337
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 1 cut(s) 1106
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 1 cut(s) 366
AfaI GTAC 3 cut(s) 23, 864, 1291
AfiI CCNNNNNNNGG 1 cut(s) 1102
AflII CTTAAG 1 cut(s) 1441
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 5 cut(s) 172, 510, 758, 883, 895
AhlI ACTAGT 1 cut(s) 602
AjnI CCWGG 2 cut(s) 84, 865
AluBI AGCT 9 cut(s) 440, 598, 641, 761, 1041, 1058, 1079, 1247, 1369
AluI AGCT 9 cut(s) 440, 598, 641, 761, 1041, 1058, 1079, 1247, 1369
Alw26I GTCTC 3 cut(s) 913, 961, 1173
AlwI GGATC 3 cut(s) 822, 835, 1337
AoxI GGCC 3 cut(s) 219, 1006, 1205
ApeKI GCWGC 4 cut(s) 197, 1244, 1247, 1327
ApoI RAATTY 1 cut(s) 1106
AspA2I CCTAGG 1 cut(s) 47
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 3 cut(s) 44, 211, 1006
AsuHPI GGTGA 3 cut(s) 329, 602, 1174
AvaII GGWCC 2 cut(s) 44, 211
AvrII CCTAGG 1 cut(s) 47
BalI TGGCCA 1 cut(s) 221
BamHI GGATCC 1 cut(s) 827
BanI GGYRCC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 498
BbvI GCAGC 4 cut(s) 184, 1234, 1256, 1314
BccI CCATC 4 cut(s) 581, 603, 1121, 1355
BceAI ACGGC 1 cut(s) 993
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 2 cut(s) 86, 867
BclI TGATCA 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 913, 961, 1173
BcuI ACTAGT 1 cut(s) 602
BfaI CTAG 6 cut(s) 48, 357, 462, 603, 1251, 1319
BfoI RGCGCY 1 cut(s) 369
BfrI CTTAAG 1 cut(s) 1441
BisI GCNGC 6 cut(s) 198, 396, 1208, 1245, 1248, 1328
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 6 cut(s) 199, 397, 1209, 1246, 1249, 1329
Bme1390I CCNGG 2 cut(s) 86, 867
Bme18I GGWCC 2 cut(s) 44, 211
BmgT120I GGNCC 3 cut(s) 44, 211, 1006
BmiI GGNNCC 2 cut(s) 367, 829
BmrFI CCNGG 2 cut(s) 86, 867
BmsI GCATC 6 cut(s) 152, 409, 835, 1029, 1231, 1408
BpiI GAAGAC 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 788
BsaBI GATNNNNATC 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 366
BsaI GGTCTC 2 cut(s) 961, 1173
BsaJI CCNNGG 3 cut(s) 47, 849, 866
BsaXI ACNNNNNCTCC 2 cut(s) 1132, 1162
Bsc4I CCNNNNNNNGG 1 cut(s) 1102
Bse1I ACTGG 2 cut(s) 929, 1128
Bse8I GATNNNNATC 1 cut(s) 488
BseBI CCWGG 2 cut(s) 86, 867
BseDI CCNNGG 3 cut(s) 47, 849, 866
BseGI GGATG 8 cut(s) 559, 592, 664, 859, 925, 1132, 1246, 1420
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 1 cut(s) 1102
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 2 cut(s) 929, 1128
BseRI GAGGAG 6 cut(s) 140, 725, 728, 1030, 1140, 1415
BseXI GCAGC 4 cut(s) 184, 1234, 1256, 1314
BsgI GTGCAG 2 cut(s) 930, 1313
Bsh1236I CGCG 2 cut(s) 398, 1210
BshFI GGCC 3 cut(s) 221, 1008, 1207
BshNI GGYRCC 1 cut(s) 365
BslFI GGGAC 2 cut(s) 1079, 1316
BslI CCNNNNNNNGG 1 cut(s) 1102
BsmAI GTCTC 3 cut(s) 913, 961, 1173
BsmFI GGGAC 2 cut(s) 1079, 1316
BsnI GGCC 3 cut(s) 221, 1008, 1207
Bso31I GGTCTC 2 cut(s) 961, 1173
Bsp143I GATC 5 cut(s) 131, 486, 619, 827, 1342
BspACI CCGC 4 cut(s) 396, 1208, 1237, 1300
BspANI GGCC 3 cut(s) 221, 1008, 1207
BspCNI CTCAG 1 cut(s) 683
BspFNI CGCG 2 cut(s) 398, 1210
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 2 cut(s) 367, 829
BspPI GGATC 3 cut(s) 822, 835, 1337
BspT107I GGYRCC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 1441
BspTNI GGTCTC 2 cut(s) 961, 1173
BsrI ACTGG 2 cut(s) 929, 1128
BssECI CCNNGG 3 cut(s) 47, 849, 866
BssMI GATC 5 cut(s) 131, 486, 619, 827, 1342
BssNI GRCGYC 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 86, 867
Bst4CI ACNGT 6 cut(s) 26, 504, 703, 744, 776, 1144
Bst6I CTCTTC 1 cut(s) 803
BstACI GRCGYC 1 cut(s) 366
BstAFI CTTAAG 1 cut(s) 1441
BstC8I GCNNGC 2 cut(s) 393, 667
BstDEI CTNAG 2 cut(s) 436, 670
BstF5I GGATG 8 cut(s) 559, 592, 664, 859, 925, 1132, 1246, 1420
BstFNI CGCG 2 cut(s) 398, 1210
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 5 cut(s) 134, 489, 622, 830, 1345
BstMAI GTCTC 3 cut(s) 913, 961, 1173
BstMBI GATC 5 cut(s) 131, 486, 619, 827, 1342
BstMWI GCNNNNNNNGC 2 cut(s) 118, 446
BstNI CCWGG 2 cut(s) 86, 867
BstNSI RCATGY 2 cut(s) 692, 796
BstSCI CCNGG 2 cut(s) 84, 865
BstUI CGCG 2 cut(s) 398, 1210
BstV1I GCAGC 4 cut(s) 184, 1234, 1256, 1314
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
BsuRI GGCC 3 cut(s) 221, 1008, 1207
BtgZI GCGATG 2 cut(s) 608, 1006
BtsCI GGATG 8 cut(s) 559, 592, 664, 859, 925, 1132, 1246, 1420
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 4 cut(s) 456, 547, 712, 1121
Cac8I GCNNGC 2 cut(s) 393, 667
CciI TCATGA 1 cut(s) 778
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 3 cut(s) 44, 211, 1006
CseI GACGC 1 cut(s) 1339
Csp6I GTAC 3 cut(s) 22, 863, 1290
CviQI GTAC 3 cut(s) 22, 863, 1290
DdeI CTNAG 2 cut(s) 436, 670
DinI GGCGCC 1 cut(s) 367
DpnI GATC 5 cut(s) 133, 488, 621, 829, 1344
DpnII GATC 5 cut(s) 131, 486, 619, 827, 1342
DraI TTTAAA 2 cut(s) 637, 838
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 1 cut(s) 803
EarI CTCTTC 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 47
Eco31I GGTCTC 2 cut(s) 961, 1173
Eco47I GGWCC 2 cut(s) 44, 211
Eco57I CTGAAG 1 cut(s) 1065
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 84, 865
EcoT14I CCWWGG 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 367
EheI GGCGCC 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 47
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 2 cut(s) 1079, 1316
FauI CCCGC 2 cut(s) 1230, 1293
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 6 cut(s) 198, 396, 1208, 1245, 1248, 1328
FokI GGATG 8 cut(s) 566, 599, 671, 866, 912, 1139, 1253, 1427
Fsp4HI GCNGC 6 cut(s) 198, 396, 1208, 1245, 1248, 1328
FspBI CTAG 6 cut(s) 48, 357, 462, 603, 1251, 1319
GlaI GCGC 1 cut(s) 367
GluI GCNGC 6 cut(s) 198, 396, 1208, 1245, 1248, 1328
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 3 cut(s) 221, 1008, 1207
HgaI GACGC 1 cut(s) 1339
HhaI GCGC 1 cut(s) 368
Hin1I GRCGYC 1 cut(s) 366
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HindIII AAGCTT 2 cut(s) 639, 1077
HinfI GANTC 3 cut(s) 230, 344, 1304
HphI GGTGA 3 cut(s) 329, 602, 1174
Hpy166II GTNNAC 3 cut(s) 9, 676, 1140
Hpy188I TCNGA 2 cut(s) 411, 1045
Hpy188III TCNNGA 3 cut(s) 462, 767, 779
Hpy8I GTNNAC 3 cut(s) 9, 676, 1140
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 2 cut(s) 459, 1153
HpyCH4III ACNGT 6 cut(s) 26, 504, 703, 744, 776, 1144
HpyCH4IV ACGT 2 cut(s) 457, 1424
HpyCH4V TGCA 9 cut(s) 200, 262, 380, 422, 665, 860, 947, 1244, 1330
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 446
HpyF3I CTNAG 2 cut(s) 436, 670
HpySE526I ACGT 2 cut(s) 457, 1424
Hsp92I GRCGYC 1 cut(s) 366
HspAI GCGC 1 cut(s) 366
KasI GGCGCC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 5 cut(s) 131, 486, 619, 827, 1342
LmnI GCTCC 2 cut(s) 1017, 1153
Lsp1109I GCAGC 4 cut(s) 184, 1234, 1256, 1314
LweI GCATC 6 cut(s) 152, 409, 835, 1029, 1231, 1408
MaeI CTAG 6 cut(s) 48, 357, 462, 603, 1251, 1319
MaeII ACGT 2 cut(s) 457, 1424
MaeIII GTNAC 1 cut(s) 1112
MalI GATC 5 cut(s) 133, 488, 621, 829, 1344
MboI GATC 5 cut(s) 131, 486, 619, 827, 1342
MboII GAAGA 6 cut(s) 101, 104, 239, 498, 820, 1058
MflI RGATCY 1 cut(s) 827
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 8 cut(s) 79, 113, 167, 520, 559, 724, 896, 1106
MluNI TGGCCA 1 cut(s) 221
Mly113I GGCGCC 1 cut(s) 366
MlyI GAGTC 1 cut(s) 1298
MmeI TCCRAC 2 cut(s) 538, 913
Mox20I TGGCCA 1 cut(s) 221
MscI TGGCCA 1 cut(s) 221
MseI TTAA 8 cut(s) 116, 254, 636, 797, 837, 977, 1194, 1442
MslI CAYNNNNRTG 2 cut(s) 148, 494
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 1247
MspCI CTTAAG 1 cut(s) 1441
MspR9I CCNGG 2 cut(s) 86, 867
MvaI CCWGG 2 cut(s) 86, 867
MvnI CGCG 2 cut(s) 398, 1210
MwoI GCNNNNNNNGC 2 cut(s) 118, 446
NarI GGCGCC 1 cut(s) 366
NdeII GATC 5 cut(s) 131, 486, 619, 827, 1342
NlaIV GGNNCC 2 cut(s) 367, 829
NmeAIII GCCGAG 2 cut(s) 318, 874
NspI RCATGY 2 cut(s) 692, 796
PagI TCATGA 1 cut(s) 778
PciI ACATGT 1 cut(s) 688
PfeI GAWTC 2 cut(s) 230, 344
PkrI GCNGC 6 cut(s) 199, 397, 1209, 1246, 1249, 1329
PleI GAGTC 1 cut(s) 1298
PluTI GGCGCC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 1298
PpuMI RGGWCCY 1 cut(s) 44
PscI ACATGT 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 84, 865
PspGI CCWGG 2 cut(s) 84, 865
PspN4I GGNNCC 2 cut(s) 367, 829
PspPI GGNCC 3 cut(s) 44, 211, 1006
PspPPI RGGWCCY 1 cut(s) 44
PsuI RGATCY 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 1247
RsaI GTAC 3 cut(s) 23, 864, 1291
RsaNI GTAC 3 cut(s) 22, 863, 1290
RseI CAYNNNNRTG 2 cut(s) 148, 494
SaqAI TTAA 8 cut(s) 116, 254, 636, 797, 837, 977, 1194, 1442
SatI GCNGC 6 cut(s) 198, 396, 1208, 1245, 1248, 1328
Sau3AI GATC 5 cut(s) 131, 486, 619, 827, 1342
Sau96I GGNCC 3 cut(s) 44, 211, 1006
SchI GAGTC 1 cut(s) 1298
ScrFI CCNGG 2 cut(s) 86, 867
SfaNI GCATC 6 cut(s) 152, 409, 835, 1029, 1231, 1408
SfoI GGCGCC 1 cut(s) 367
SinI GGWCC 2 cut(s) 44, 211
SmiMI CAYNNNNRTG 2 cut(s) 148, 494
SmlI CTYRAG 2 cut(s) 767, 1441
SmoI CTYRAG 2 cut(s) 767, 1441
SpeI ACTAGT 1 cut(s) 602
Sse9I AATT 8 cut(s) 79, 113, 167, 520, 559, 724, 896, 1106
SsiI CCGC 4 cut(s) 396, 1208, 1237, 1300
SspDI GGCGCC 1 cut(s) 365
SspI AATATT 1 cut(s) 205
SspMI CTAG 6 cut(s) 48, 357, 462, 603, 1251, 1319
StyD4I CCNGG 2 cut(s) 84, 865
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 6 cut(s) 26, 504, 703, 744, 776, 1144
TaiI ACGT 2 cut(s) 460, 1427
TaqI TCGA 3 cut(s) 13, 750, 1387
TasI AATT 8 cut(s) 79, 113, 167, 520, 559, 724, 896, 1106
TatI WGTACW 2 cut(s) 21, 1289
TauI GCSGC 2 cut(s) 398, 1210
TfiI GAWTC 2 cut(s) 230, 344
Tru1I TTAA 8 cut(s) 116, 254, 636, 797, 837, 977, 1194, 1442
Tru9I TTAA 8 cut(s) 116, 254, 636, 797, 837, 977, 1194, 1442
TscAI CASTG 4 cut(s) 456, 547, 712, 1128
TseI GCWGC 4 cut(s) 197, 1244, 1247, 1327
TspDTI ATGAA 7 cut(s) 233, 240, 296, 512, 914, 1055, 1350
TspGWI ACGGA 1 cut(s) 1171
TspRI CASTG 4 cut(s) 456, 547, 712, 1128
Vha464I CTTAAG 1 cut(s) 1441
VpaK11BI GGWCC 2 cut(s) 44, 211
XapI RAATTY 1 cut(s) 1106
XbaI TCTAGA 1 cut(s) 461
XceI RCATGY 2 cut(s) 692, 796
XmaJI CCTAGG 1 cut(s) 47
XspI CTAG 6 cut(s) 48, 357, 462, 603, 1251, 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.